Immediate Post-Treatment Recovery Formulations and Methods

Abstract

A skin care composition and method for improving a patient's temporary post-treatment discomfort after a skin rejuvenation treatment and creating a healing-promoting environment to increase the likelihood that the patient will return for multiple treatments. The skin care composition includes an emulsion formed from active ingredients in a cosmetically acceptable carrier. The active ingredients include an effective amount of one or more neurobeautifying ingredients selected to increase the concentration of β-endorphin, decrease the concentration of CGRP, and block TRPV1 activity, and an effective amount of a prebiotic selected to nourish the native microbiome in human skin.

Description

[Technical field]

[0001] [CROSS REFERENCE TO RELATED APPLICATIONS] This application claims priority to U.S. Application No. 17 / 697,779, filed March 17, 2022, entitled "IMMEDIATE POST-PROCEDURE RECOVERY FORMULATION AND METHOD," which is incorporated by reference in its entirety herein as an example.

[0002] Novel aspects of the present disclosure relate to the field of skin care, and more particularly to compositions and associated methods for ameliorating pain and discomfort following skin rejuvenation procedures that injure the skin and creating an environment that promotes healing. [Background technology]

[0003] Skin aging, caused by intrinsic factors like genetics, and extrinsic factors like environmental aggressors, can lead to visible changes in the skin surface, including fine lines, wrinkles, pigmentation, uneven skin tone, and tactile roughness. Skin rejuvenation procedures are performed to treat such aging skin concerns. Three common types of skin rejuvenation procedures include those that injure the skin through mechanical means, chemical attacks, and / or the use of energy-based devices. These common skin rejuvenation procedures vary in their strength, depth of penetration into the skin, and downtime after the procedure. Mechanical methods include rejuvenation techniques with microneedle devices, and / or resurfacing techniques that utilize dermabrasion and dermaplaning. Chemical methods generally involve the application of various acidic solutions to remove layers of the skin. The depth of treatment depends on factors such as the acidity of the solution, the type of chemical used, and the treatment time. Acids commonly used in chemical peels include alpha hydroxy acids and beta hydroxy acids. Energy-based device techniques commonly use lasers such as carbon dioxide (CO2), erbium (Er)-doped yttrium aluminum garnet (YAG), or radio frequency (RF) emitters. By controlling the wavelength and amount of energy used, energy-based devices can penetrate one or more layers of the skin. Each of these skin rejuvenation techniques injures the skin, which triggers the body to initiate the healing process, increases blood flow, and upregulates extracellular matrix proteins, including collagen and elastin, resulting in improved skin health and appearance. With most procedures, the skin responds and improves after a single treatment, but optimal results occur after a series of prescribed treatments spaced several weeks apart.

[0004] The previously mentioned skin rejuvenation procedures can be classified based on the amount of trauma caused to the skin. Non-ablative procedures are fairly mild, treating the epidermis and not destroying the skin. Ablative procedures are more aggressive, destroying the skin and can damage both the epidermis and dermis. Semi-ablative procedures, which can be performed using hybrid fractional lasers, target both the epidermis and dermis but are less traumatic than fully ablative procedures. One exemplary hybrid fractional laser combines non-ablative and ablative energy wavelengths, with sequential ablation of the epidermis followed by dermal coagulation to denature collagen in the dermis layer.

[0005] These skin rejuvenation procedures require multiple treatments over a period of time to achieve full benefit and satisfaction. However, these procedures collectively lead to patient discomfort due to the nature of their mechanism of action. These procedures can leave patients with skin that is irritated and painful. The skin appears very red with erythema, is warm to the touch, and is itchy. Even if the results are noticeable, a negative experience with these procedures decreases the likelihood that a patient will return to complete an optimal course of treatment.

[0006] [Means for solving the problem] A novel aspect of the present disclosure is directed to a method for improving discomfort and creating a healing-promoting environment after a skincare rejuvenation procedure that injures the skin, comprising increasing the concentration of beta-endorphin in the skin to reduce pain signal transmission, decreasing the concentration of calcitonin gene-related peptide (CGRP) to reduce pain sensitivity, blocking transient receptor potential cation channel subfamily V member (TRPV1) activity to reduce nociceptor sensitivity, and nourishing the native microbiome in the human skin to promote barrier repair of the skin.

[0007] The novel aspect of the present disclosure is also directed to a skin care composition for improving discomfort and creating a healing-promoting environment after a skin care rejuvenation procedure that damages the skin.The skin care composition is formed from an active ingredient mixed with a cosmetically acceptable carrier.The active ingredient comprises an effective amount of one or more neurobeautifying ingredients selected to increase the concentration of beta-endorphins to reduce the transmission of pain signals, decrease the concentration of CGRP to reduce pain perception, and block TRPV1 activity to reduce nociceptor sensitivity, and an effective amount of prebiotics selected to nourish the native microbiome in the human skin to promote barrier repair of the skin.

[0008] The novel aspect of the present disclosure is also directed to a method for rejuvenating skin.The method includes carrying out a skincare rejuvenation treatment that injures skin, and providing a skincare composition for improving discomfort after the skincare rejuvenation treatment and creating an environment that promotes healing.The skincare composition can be an emulsion formed from an active ingredient in a cosmetically acceptable carrier, the active ingredient includes an effective amount of one or more neurobeautifying ingredients selected to increase the concentration of beta-endorphin, decrease the concentration of CGRP, and block TRPV1 activity, an effective amount of a prebiotic selected to nourish the native microbiome in human skin, an effective amount of a moisturizing agent, and an effective amount of an antioxidant.

[0009] Other aspects, embodiments and features of the present invention will become apparent from the following detailed description of the invention when considered in conjunction with the accompanying figures, in which each identical or substantially similar component that is illustrated in the various figures is represented by a single numeral or designation. For clarity, not every component is shown in every figure. Also, not every component of each embodiment of the present invention is shown unless illustration is necessary for those skilled in the art to understand the invention.

[0010] The novel features believed to be characteristic of the invention are set forth in the appended claims, however the invention itself, together with its preferred modes of use, further objects and advantages, will best be understood by reference to the following detailed description of illustrative embodiments when read in conjunction with the accompanying drawings. [Brief description of the drawings]

[0011] [Figure 1A] 1 is a chart depicting an exemplary wound healing process experienced following a conventional skin rejuvenation treatment utilizing lasers and microneedles. [Figure 1B] 1 is a chart depicting an exemplary wound healing process experienced following a conventional skin rejuvenation treatment utilizing lasers and microneedles. [Diagram 2] 1 is a flow chart of a method for ameliorating discomfort after a traumatic skin rejuvenation procedure according to an exemplary embodiment; and [Diagram 3] 1 is a flowchart of a method for rejuvenating skin according to an exemplary embodiment. Summary of the Invention [Problem to be solved by the invention]

[0012] More invasive skin rejuvenation procedures usually provide the most obvious benefits, especially when repeated over multiple treatment sessions. However, these skin rejuvenation procedures can usually cause a significant level of pain and discomfort, in the form of irritation, burning, redness, itching, etc. Such pain and discomfort can discourage patients from returning for additional treatments and mean less than optimal results for the patient. Traditional post-treatment skin care regimens consist of moisturizers with optional sunscreen, which do not adequately ameliorate the painful side effects of treatment. Other post-treatment skin care regimens are complex and expensive regimens that require the application of multiple different post-treatment products to address different post-treatment symptoms. For example, at least one traditional post-treatment skin care regimen requires four separate products: a cleanser, a repair complex containing growth factor ingredients to stimulate collagen formation, a hydrating cream, and a moisturizer. Adherence to this post-treatment skin care regimen would delay the introduction of soothing ingredients, exposing the patient to a longer period of discomfort and at a time when healing of the skin barrier is well underway. Many other conventional skin care compositions for application following skin rejuvenation procedures are anhydrous and / or occlusive and focus on moisturizing the skin, but do not create an environment that promotes optimal healing and alleviates patient discomfort.

[0013] To the applicant's knowledge, there is no prior art that discloses a post-treatment product that has a unique neuro-cosmetic ingredient that soothes the skin and promotes skin rejuvenation and that can be applied within at least 15 minutes, more particularly within 10 minutes, of the skin rejuvenation treatment. Similarly, the applicant is not aware of a post-treatment product that has a unique neuro-cosmetic ingredient that soothes the skin and promotes skin rejuvenation and that can be applied within at least 5 minutes, more particularly approximately immediately after the skin rejuvenation treatment. Whereas the prior art does not include disclosures regarding the delivery of neuro-cosmetic ingredients, the present invention discloses a skin care formulation that delivers a unique neuro-soothing ingredient to relieve patient discomfort within at least 15 minutes, more particularly within 10 minutes, of the skin rejuvenation treatment.

[0014] The novel aspects of the present disclosure recognize the need for a skin care formulation that can be applied to human skin as a post-treatment skin care formulation that can mitigate various unpleasant side effects of skin rejuvenation treatments by stimulating the body's natural biological response to injury with a single product using selected ingredients. Importantly, the novel skin care formulation can be applied immediately after a skin rejuvenation treatment, potentially while the skin barrier is substantially compromised, thereby promoting optimal delivery and uptake of neurocosmetics that target specific biological responses to reduce pain perception, prebiotics that promote a balanced microbiome, optional moisturizers that soften the skin, and antioxidants to reduce the presence of free radicals. Additionally, the skin care formula is free of topical anesthetics and solvent alcohols that can cause irritation and toxicity complications. Thus, by incorporating specific ingredients that promote a balanced microbiome, moisturize the skin, and reduce free radicals, an environment that promotes healing is created. Additionally, the healing-promoting environment also excludes ingredients that are believed to impede healing, such as anesthetics, solvent alcohols, and occlusive carriers.

[0015] Ablative skin care rejuvenation treatments destroy the skin, causing wounds that penetrate the epidermis and dermis, thereby stimulating collagen production in the deeper layers of the skin.Ablative skin care treatments are therefore ideal for treating deeper wrinkles, discoloration, texture defects, and deeper acne scars.Exemplary devices that can be used to provide ablative skin care treatments include CO2, erbium, and erbium / YAG lasers.

[0016] Non-ablative skin care treatments also stimulate collagen production, but injure the dermis layer without damaging the epidermis.These non-ablative skin care treatments are ideal for treating fine lines, mild scars, mild discoloration, and superficial sunburn.The exemplary devices that can be used to provide non-ablative skin care treatments include non-ablative fractional lasers and non-ablative RF transmitters to induce degeneration and acute inflammation of the dermis layer.

[0017] Semi-ablative rejuvenating skin care treatments that result in some degree of ablation of the epidermis layer and coagulation of the dermis layer can also stimulate collagen production in deeper layers of the skin, but without the long recovery period required by ablative skin care treatments. In some embodiments, semi-ablative skin care treatments are provided by a hybrid fractional laser, which is an energy-based device that includes ablative and non-ablative energy wavelengths working in concert to result in sequential ablation of the epidermis followed by coagulation of the dermis (i.e., denaturation of dermal collagen).

[0018] While the skin rejuvenation treatments described above involve energy-based devices, in other embodiments, the skin rejuvenation treatment can include mechanical rejuvenation and chemical resurfacing, either alone or in combination. Examples of mechanical rejuvenation treatments include dermaplaning, dermabrasion, and microneedling. Chemical resurfacing treatments include acid peels.

[0019] FIG. 1A is a chart depicting an exemplary wound healing process that a patient experiences after a conventional skin rejuvenation procedure. In particular, chart 100a depicts the various symptoms and observable histological changes that a patient experiences throughout a seven-day recovery period after a laser-based skin rejuvenation procedure. In some cases, symptoms and recovery periods may vary based on the extent of damage and skin quality, as well as the age of the patient.

[0020] The skin rejuvenation procedure ends at time = 0, and within the first 24 hours the patient experiences sensations of heat, pain, itching, irritation, swelling, and dryness. Histological changes include ablation of the epidermis with dermal degeneration of collagen and coagulation of the dermis. In addition, trabeculae of epidermal and dermal tissue are removed, and microthermal zones (MTZ) of injury are observed in the epidermis and dermis.

[0021] Between 24 and 72 hours after the skin rejuvenation procedure, the patient experiences sensations of swelling, dryness, and discomfort. Histologically, they experience epidermal regeneration characterized by the replacement of damaged keratinocytes by healthy keratinocytes. Microscopic epidermal necrotic debris (MENDS) is observed in the epidermis, and subepidermal clefts form. Perivascular inflammatory infiltrates begin.

[0022] Between 72 hours and 5 days, patients experience sensations of swelling and dryness. Histologically, the MENDS migrate out of the epidermis and are found between the epidermis and the stratum corneum. Dyskeratinized cells are present in the epidermis and regression of the subepidermal clefts occurs. Distinct cones of homogenized collagen are observed.

[0023] Between 5 and 7 days, patients experience sensations of swelling and dryness, along with peeling and scaling. Histologically, MENDS are observed within the stratum corneum and inflammation of the dermis around lymphatics and blood vessels. Distinct cones of homogenized collagen are maintained.

[0024] After 7 days, the patient continues to experience a sensation of dryness and flaking skin. Histologically, the MENDS are sloughed off and regeneration of the dermis begins. Collagen III production around the MTZ is observed along with regression of the inflammatory infiltrate.

[0025] FIG. 1B is a chart depicting an exemplary wound healing process experienced by a patient after another conventional skin rejuvenation procedure. In particular, chart 100b depicts the various symptoms and observable histological changes experienced by a patient throughout an eight-month recovery period following a microneedle procedure. In some cases, symptoms and recovery periods may vary depending on the extent of injury, the quality of the skin, and the age of the patient.

[0026] The skin rejuvenation procedure is completed at time = 0, and within the first 2-8 hours the patient experiences sensations of pain, swelling, heat and redness. Histologically, the skin is seen to be in an inflammatory phase. Localized damage of superficial blood vessels and collagen bundles is observed along with the release of platelets and neutrophils.

[0027] The patient continues to experience swelling, redness, pain, and a feeling of heat for anywhere from 2-8 hours to up to 5 days. Histologically, rapid healing of the microchannels is observed. In addition, transdermal migration of keratinocytes occurs.

[0028] Over the next 5-7 days, the patient continues to experience swelling and redness. Histological samples show evidence of an inflammatory phase in the skin.

[0029] Between 7 days and 8 months, the patient no longer experiences sensations due to the rejuvenation process, but the histological changes continue. In particular, an infiltration of monocytes / macrophages is observed. Platelet growth factors are released and keratinocytes remodel the basement membrane (i.e. laminin and collagen). Fibroblasts, fibronectin and collagen production are also observed.

[0030] Histologic changes after 8 months included skin remodeling, epidermal thickening, and neoformation of collagen I, III, and IV.

[0031] The wound healing continuum depicted in Figures 1A and 1B is illustrative and not meant to be limiting. Although the sensory and histological changes are generally similar regardless of the type of skin rejuvenation procedure, i.e., ablative, non-ablative, or semi-ablative (whether by hybrid fractional laser or other methods), the actual length of recovery and sensory severity will vary depending on the extent of injury to the skin, the age, and the health of the patient.

[0032] The skin care formulations described herein are formed from a plurality of active ingredients mixed with a cosmetically acceptable carrier. The cosmetically acceptable carrier can be a non-occlusive or semi-occlusive lotion or cream. In certain embodiments, the cosmetically acceptable carrier is a semi-permeable lotion or cream, which helps reduce water loss like occlusive type carriers, but allows the skin to breathe. The breathability of the carrier is a factor in improving the skin's environment by reducing the chance of infection and promoting healing. Again, the cosmetically acceptable carrier is not an occlusive lotion or cream, which prevents the breathability of the skin. Non-limiting examples of said cosmetically acceptable carriers can include conventional non-occlusive oil-in-water emulsions, water-in-oil emulsions, water-in-silicone emulsions, or oil-in-water emulsions in combination with a film former. The carrier base can form about 65-99.6 wt% of the formulation. Non-limiting examples of film formers include synthetic or natural resins, copolymers of acrylic acid or acrylic acid esters, chitosan and its derivatives, cellulose polymers, polyesters, polyurethanes, starch copolymers, styrene copolymers, VA and / or VP polymers, and / or waxes. More specific examples of film formers include C12-22 alkyl methacrylate copolymers, ethyl cellulose, butyl ester of PVM / MA copolymer, VP / eicosene copolymer, trimethylsiloxysilicate, adipic acid / neopentyl glycol / trimellitic anhydride copolymer, and the like.

[0033] The active ingredient includes one or more neurobeautifying ingredients. Neurobeautifying ingredients are topically applicable bioactive ingredients that can affect the innervation sites of human skin by penetrating the skin barrier and / or through signal transduction mechanisms. The neurobeautifying ingredients included in the exemplary skin care formulations described herein are selected to reduce inflammation, itch, pain, and irritability through three main modes of action: increasing the concentration of beta-endorphins, decreasing the concentration of CGRP, and blocking the activity of TRPV1. Blocking the activity of TRPV1 can include blocking the activation of TRPV1 or blocking the reception of TRPV1.

[0034] Beta-endorphin is a peptide that is an agonist of opioid receptors in the human body. Beta-endorphin is involved in nociception, i.e., the perception of pain, in the central and peripheral nervous systems. When beta-endorphin binds to opioid receptors, it inhibits the release of neurotransmitters responsible for pain signaling. In addition, beta-endorphin inhibits the release of GABA neurotransmitters, which inhibit the release of dopamine. Thus, increased concentrations of beta-endorphin lead to the release of more dopamine, which in turn leads to a greater sense of relaxation and well-being.

[0035] The beta-endorphin is produced by keratinocytes and sensory neurons. Increasing the production of beta-endorphin in the skin can be achieved by including in the skin care treatment one or more of the following topical active ingredients: curry plant extract, carrot root extract, French lavender extract, chrysanthemum maritimum extract, and acetyl dipeptide-1 cetyl ester.

[0036] TRPV1 is a polymodal nociceptor, or pain receptor, present in neurons and keratinocytes innervating the skin. Skin irritation or injury, such as that caused by skin rejuvenation procedures, triggers the release of inflammatory mediators that increase the sensitivity of TRPV1 and other nociceptors to stimulation. CGRP is an amino acid peptide that is produced by human neurons upon neuronal activation by specific agonists of TRPV1 during injury. Thus, CGRP is thought to be an inflammatory mediator associated with pain perception.

[0037] Targeting TRPV1 with antagonists reduces the sensation of pain caused by inflammatory and neuropathic pain. Targeting TRPV1 can also reduce the concentration of CGRP. TRPV1 activity can be reduced by competitive or non-competitive antagonists. These antagonists can be provided by one or more of the active ingredients of Ningyotake extract, which blocks TRPV1 receptors, and Prickly Pear cactus Ficus indica callus culture extract, which blocks TRPV1 activation.

[0038] Thus, the one or more neurobeautifying ingredients include one or more of curry plant flower, cinnamon extract, carrot root extract, opuntia ficus indica callus culture extract or acetyl dipeptide-1 cetyl ester. In one embodiment, the one or more neurobeautifying ingredients are present in an effective amount in the cosmetic formulation. As used herein, the term "effective amount" refers to a sufficient amount of a compound that can significantly induce an improvement in the condition being treated, within the sound judgment of the skilled artisan, but low enough to avoid undesirable side effects. The effective amount of the compound may vary depending on the particular condition being treated, the age and condition of the biological subject being treated, the severity of the condition, the duration of the treatment, and other factors within the knowledge and expertise of the skilled artisan.

[0039] The active ingredients of the skin care formulation also include an effective amount of prebiotics that can nourish the natural microbiome of human skin to promote healthy skin. Human skin hosts a variety of microorganisms that form the microbiome, i.e., good bacteria. The presence of good bacteria on the skin can prevent the growth of bad bacteria on the skin. Unhealthy skin due to an imbalance in the microbiome reduces the barrier function and causes hypersensitivity. Non-limiting examples of said prebiotics include plant fibers such as beta-glucan and inulin, Laminaria digitata extract, Chlorella extract, and Cassava extract.

[0040] The active ingredient of the skin care formulation also includes an effective amount of a moisturizing agent. The moisturizing agent can be a lipophilic emollient and / or a moisturizing agent. Examples of the moisturizing agent include at least one of glycerin, saccharide isomerate, sorbitol, sodium hyaluronate, jojoba esters, squalene, cholesterol, natural vegetable oils and their derivatives, phytosphingosine, ceramide, watermelon fruit extract, lentil fruit extract, apple fruit extract, Saccharomyces cerevisiae extract and Bacillus ferment. Bacillus ferment can be obtained from marine microorganisms.

[0041] The active ingredients of the skin care formulation also include an effective amount of an antioxidant, non-limiting examples of which may include one or more of plant polyphenols, including flavonoids and phenolic acids, carotenoids, ubiquinone, tocopherol or a derivative thereof, ascorbic acid or a derivative thereof, including tetrahexyldecyl ascorbic acid, bactiol, and acetylgingerone.

[0042] Thus, an exemplary skin care formulation is an emulsion formed from a cosmetically acceptable carrier and an active ingredient, comprising an effective amount of a neurobeautifying ingredient and an effective amount of one or more prebiotics. In another embodiment, the active ingredient may also comprise an effective amount of one or more moisturizing agents and one or more antioxidants.

[0043] In certain embodiments, the effective amount of one or more neurocosmetic ingredients for ameliorating pain and discomfort following a semi-ablative skin rejuvenation procedure is between 0.0526-2.629 wt% of the skin care formulation, more particularly between 0.213-1.893 wt% of the skin care formulation. In certain embodiments, the effective amount of neurocosmetic ingredients for ameliorating pain and discomfort following a semi-ablative skin rejuvenation procedure is about 1.05 wt% of the skin care formulation.

[0044] In a more specific embodiment, the one or more neuro-beautifying ingredients include an effective amount of one or more ingredients selected to increase the concentration of beta-endorphin. The effective amount of the one or more ingredients selected to increase the concentration of beta-endorphin can be between 0.0158 and 0.791 wt% of the skin care formulation, more specifically between 0.063 and 0.570 wt% of the skin care formulation. In this more specific embodiment, the one or more neuro-beautifying ingredients include an effective amount of one or more ingredients selected to block TRPV1 receptors. The effective amount of the one or more ingredients selected to block TRPV1 receptors can be between 0.037 and 1.838 wt% of the skin care formulation, more specifically between 0.147 and 1.323 wt% of the skin care formulation.

[0045] One or more of the active ingredients may have a secondary mechanism of action that is beneficial for incorporation into skin care compositions to improve discomfort after skin rejuvenation procedures. For example, Prickly Pear Ficus Indica culture extract and Saccharomyces cerevisiae extract also downregulate matrix metalloproteinase-1 (MMP1), an enzyme responsible for the degradation of extracellular matrix, especially types I, II and III interstitial collages. Carrot root extract inhibits the release of CGRP and protects the skin from erythema (redness). Ningyotake extract reduces discomfort and inflammation and protects the skin from heat stress. Acetyl dipeptide-1 cetyl ester also reduces heat sensitivity. Curry plant extract can counter the effects of cortisol and strengthen the skin barrier by improving the production of adhesive proteins.

[0046] Conventional formulations for application after skin rejuvenation procedures contain cytotoxic ingredients that are harmful to cells, such as solvent-based alcohols, including ethanol or isopropyl alcohol. Alcohol strips the skin of lipids and moisturizing factors, which prolongs the wound healing process. Such conventional formulations often also contain local anesthetics, such as benzocaine, to reduce the sensation of pain and irritation. However, the use of local anesthetics is believed to increase the risk of developing local anesthetic system toxicity (LAST). Furthermore, local anesthetics, such as benzocaine, lidocaine, and tetracaine, only produce a temporary numbing effect due to their mechanism of action that targets sodium ion channels on the inner surface of nerve cell membranes. However, once the numbing effect wears off, known irritation reactions may appear. These side effects, such as itching, redness, and scaling of the skin, can cause enough discomfort to deter patients from returning for additional treatment. Thus, in some embodiments, Applicant's novel skin care formulations lack ingredients that retard wound healing, such as alcohol and local anesthetics.

[0047] 2 is a flow chart of a process for improving discomfort after a skin rejuvenation procedure that wounds the skin, according to an exemplary embodiment. In one embodiment, the process depicted in flow chart 200 begins in response to application of a skin care formulation to the skin after the skin has been subjected to a rejuvenation procedure. Non-limiting examples of rejuvenation procedures can include ablative, non-ablative, or semi-ablative procedures, as described above.

[0048] The process begins in step 202 by increasing the concentration of beta-endorphins in the skin. The concentration of beta-endorphins can be increased by an effective amount of one or more neuro-beautic ingredients, examples of which can include, but are not limited to, one or more of curry plant extract, ginseng extract, carrot root extract, prickly pear cactus Ficus indica culture extract, and acetyl dipeptide-1 cetyl ester. In step 204, the concentration of CGRP is reduced by an effective amount of one or more neuro-beautic ingredients. In a non-limiting embodiment, the concentration of CGRP is reduced by carrot root extract. In step 206, TRPV1 activity is blocked by an effective amount of one or more neuro-beautic ingredients. TRPV1 activity can be blocked by blocking TRPV1 activation or by blocking TRPV1 receptor. TRPV1 activation can be blocked by an effective amount of prickly pear cactus Ficus indica culture extract. TRPV1 receptor can be blocked by an effective amount of ginseng extract. In step 208, the native microbiome of the human skin is nourished. The native microbiome can be nourished with an effective amount of a prebiotic, an example of which can be inulin.

[0049] In one or more embodiments, the process depicted in flowchart 200 can include the additional step of increasing the moisture content of the skin with an effective amount of a moisturizing agent to promote barrier repair of the epidermis. The moisturizing agent can be a lipophilic emollient and / or humectant, examples of which can include glycerin, saccharide isomerates, sorbitol, sodium hyaluronate, jojoba esters, squalene, cholesterol, natural vegetable oils and their derivatives, phytosphingosine, ceramides, watermelon fruit extract, lentil fruit extract, apple fruit extract, Saccharomyces cerevisiae extract, and Bacillus ferment.

[0050] Additionally, in one or more embodiments, the process depicted in flowchart 200 can include another step of reducing the presence of free radicals with an effective amount of an antioxidant, non-limiting examples of which can include one or more of plant polyphenols, including flavonoids and phenolic acids, carotenoids, ubiquinones, tocopherol and tocopherol acetate, ascorbic acid or derivatives thereof, including tetrahexyldecyl ascorbic acid, bactiol, and acetyl zingerone.

[0051] The skin care formulation comprises an effective amount of a moisturizer to increase the moisture content of the skin, an effective amount of at least one neurobeautic ingredient selected to increase the concentration of beta-endorphins, decrease the concentration of CGRP, and block TRPV1 receptors, and an effective amount of a prebiotic selected to nourish the native microbiome of the human skin to promote skin barrier repair.

[0052] The skin care formulation may also include an effective amount of one or more moisturizers and an effective amount of one or more antioxidants. In some embodiments, the application step is performed within 0-48 hours of the skin rejuvenation treatment. In other embodiments, the application step is performed less than 24 hours of the skin rejuvenation treatment. In certain embodiments, the application step is performed immediately, i.e., 0 hours, after the skin rejuvenation treatment.

[0053] Absorption of the skin care formulation into the skin induces the steps of increasing, decreasing, blocking, and replenishing in the skin, i.e., steps 202, 204, 206, and 208 in Figure 2. Absorption of the skin care formulation into the skin can also cause an increase in hydration and a decrease in the presence of free radicals if the skin care formulation contains a moisturizer and an antioxidant, respectively.

[0054] In one or more embodiments, the process depicted in flowchart 200 can include an initial step of applying a skin care formulation to the skin, the skin care formulation configured to ameliorate discomfort after a skin rejuvenation procedure that damages the skin. The skin care formulation can be applied to the skin within at least 15 minutes of the skin rejuvenation procedure, or within at least 10 minutes of the skin rejuvenation procedure. In another embodiment, the skin care formulation can be applied within at least 5 minutes of the skin rejuvenation procedure, or substantially immediately after the skin rejuvenation procedure if the skin barrier is compromised. For best results, the skin care formulation can be applied three times a day for at least three days, but up to seven days for skin rejuvenation procedures with deeper penetration depths.

[0055] FIG. 3 is a flow chart of a method of rejuvenating skin according to an exemplary embodiment. Flow chart 300 begins at step 302 by performing a skin rejuvenation treatment on the skin. The skin rejuvenation treatment can be an ablative treatment, a non-ablative treatment, or a semi-ablative treatment. At step 304, a skin care composition is provided for application to the skin within a predetermined period of time. The skin care composition creates an environment upon application that promotes healing and improves pain and discomfort. In a non-limiting embodiment, the predetermined period of time is between 0 and 48 hours, more specifically less than about 24 hours, to improve discomfort after the skin care rejuvenation treatment. In more specific embodiments, the predetermined period of time is between 0 and 12 hours, or between 0 and 6 hours. In yet another specific example, the predetermined period of time is less than 15 minutes, less than 5 minutes, or substantially immediately after the rejuvenation treatment. The skin care composition is an emulsion formed of active ingredients in a cosmetically acceptable carrier. The active ingredients can include an effective amount of a neurobeautic ingredient, an effective amount of a prebiotic, and optionally effective amounts of a moisturizer and an antioxidant.

[0056] Although the embodiments of the present invention have been described with reference to some elements, any elements described in the embodiments described herein are exemplary and may be omitted, substituted, added, combined, or rearranged as appropriate to form new embodiments. Those skilled in the art will recognize upon reading this specification that such additional embodiments are effectively disclosed herein. For example, if the disclosure describes a property, structure, size, shape, arrangement, or composition of an element, or a process for making or using an element or combination of elements, the property, structure, size, shape, arrangement, or composition may also be incorporated into any other element or combination of elements described herein, or a process for making or using an element or combination of elements, to provide additional embodiments.

[0057] Further, when an embodiment is described herein as consisting of an element or elements, additional embodiments can consist essentially of or consist of that element or elements. Also, although the open-ended term "comprises" is generally used herein, additional embodiments can be formed by substituting the term "consisting essentially of" or "consisting of."

[0058] Although the present invention has been shown and described with reference to particular preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention.For example, the present disclosure describes applying the skin care composition described herein after a rejuvenating skin care treatment, such as non-ablative, semi-ablative, and ablative rejuvenating skin care treatment.However, the claimed method and skin care composition may also be applied after other forms of skin-based treatment that cause similar damage, such as Mohs surgery and photodynamic therapy.The inventors expect that those skilled in the art will adopt such variations as appropriate, and the inventors intend to carry out the invention in other ways than as specifically described herein.Accordingly, the present invention includes all modifications and equivalents of the subject matter described in the claims appended hereto as permitted by applicable law.Furthermore, any combination of the above-described elements in all possible variations thereof is encompassed by the present invention unless otherwise indicated herein or otherwise clearly contradicted by context.

Claims

1. 1. A method for improving discomfort and creating an environment that promotes healing after a skin rejuvenation procedure that injures the skin, comprising: increasing a concentration of beta-endorphin in the skin to reduce the transmission of pain signals; Reducing the concentration of CGRP to reduce pain sensitivity; Blocking TRPV1 activity to reduce nociceptor sensitivity; and nourishing the native microbiome of the human skin to promote barrier repair of the skin; A method comprising:

2. 10. The method of claim 1 further comprising:

20. A method comprising increasing the moisture content of the skin with an effective amount of a moisturizing agent to promote barrier repair of the skin, the moisturizing agent being at least one selected from glycerin, saccharide isomerates, sorbitol, sodium hyaluronate, jojoba esters, squalene, cholesterol, natural vegetable oils and derivatives thereof, phytosphingosine, ceramide, watermelon fruit extract, lentil fruit extract, apple fruit extract, Saccharomyces cerevisiae extract, and Bacillus ferment.

3. 10. The method of claim 1 further comprising:

16. A method comprising reducing the presence of free radicals with an effective amount of an antioxidant, said antioxidant being at least one of plant polyphenols, carotenoids, ubiquinone, tocopherol, tocopherol acetate, ascorbic acid, derivatives of ascorbic acid, bactiol, and acetylgingerone.

4. 3. The method of claim 2 further comprising: applying a skin care formulation to the skin, the skin care formulation comprising the effective amount of a moisturizer to increase the water content of the skin, an effective amount of at least one neurobeautifying ingredient selected to increase a concentration of beta-endorphins, decrease a concentration of CGRP, and block a TRPV1 receptor, and an effective amount of a prebiotic selected to nourish the native microbiome of the human skin; The method, wherein the absorption of the skin care formulation induces the steps of increase, decrease, blockage, and replenishment in the skin.

5. 5. The method of claim 4, wherein the applying step is performed within at least 15 minutes after the skin rejuvenation treatment.

6. 6. The method of claim 5, wherein the at least one neurobeautifying ingredient comprises at least one of curry plant extract, ginseng extract, carrot root extract, prickly pear cactus Ficus indica culture extract, or acetyl dipeptide-1 cetyl ester.

7. 5. The method of claim 4, wherein the prebiotic comprises one or more of beta-glucan, inulin, Laminaria digitata extract, Chlorella extract, and Cassava extract.

8. 10. The method of claim 1, wherein the skin rejuvenation treatment is either a non-ablative treatment, a semi-ablative treatment, or an ablative treatment.

9. 10. The method of claim 1, which is carried out in the absence of local anesthetics and solvent alcohols.

10. 2. The method of claim 1, wherein increasing the concentration of beta-endorphin comprises stimulating the production of beta-endorphin by keratinocytes and sensory neurons of the skin.

11. 10. The method of claim 1, wherein the concentration of CGRP is decreased by blocking the TRPV1 receptor with an antagonist provided in the skin care formulation.

12. A skin care composition for improving discomfort after a skin rejuvenation procedure and creating a healing-promoting environment, comprising: an active ingredient mixed with a cosmetically acceptable carrier, an effective amount of one or more neuro-cosmetic ingredients selected to increase the concentration of beta-endorphin, decrease the concentration of CGRP, and block TRPV1 activity; and an effective amount of a prebiotic selected to nourish the native microbiome of the human skin to promote barrier repair of the skin; It includes, A skin care composition comprising the active ingredient.

13. 13. The skin care composition of claim 12, wherein the active ingredient further comprises an effective amount of a moisturizer.

14. 14. The skin care composition of claim 13, wherein the moisturizing agent comprises at least one of jojoba esters, cholesterol, phytosphingosine, ceramide, watermelon fruit extract, lentil fruit extract, and apple fruit extract.

15. 13. The skin care composition of claim 12, wherein the one or more neurobeautifying ingredients comprise an effective amount of at least one of curry plant extract, amaranth extract, carrot root extract, prickly pear cactus Ficus indica culture extract, or acetyl dipeptide-1 cetyl ester.

16. 13. The skin care composition of claim 12, wherein the prebiotic comprises an effective amount of at least one of beta-glucan, inulin, Laminaria digitata extract, Chlorella extract, and Cassava extract.

17. 13. The skin care composition of claim 12, wherein the skin rejuvenation treatment is one of a non-ablative treatment, a semi-ablative treatment, or an ablative treatment.

18. 13. The skin care composition of claim 12, wherein the cosmetically acceptable carrier is either a water-in-oil emulsion, a water-in-silicone emulsion, or an oil-in-water emulsion.

19. 13. The skin care composition of claim 12, wherein the active ingredient further comprises an effective amount of an antioxidant, the antioxidant being at least one of ubiquinone, tocopherol, tocopherol acetate, tetrahexyldecyl ascorbate, and acetylgingerone.

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