Muscle-targeting complexes and formulations for treating myotonic dystrophy - Patent Application 20070233334

Abstract

Aspects of the disclosure relate to conjugates, and other aspects relate to formulations (e.g., aqueous, lyophilized forms) comprising such conjugates comprising an oligonucleotide (e.g., one useful for targeting DMPK) covalently linked to an antibody (e.g., an anti-TfR1 antibody).

Description

[Technical field]

[0001] Related Applications This application claims the benefit under 35 U.S.C. § 119(e) of the filing date of U.S. Provisional Application No. 63 / 331,727, entitled "MUSCLE TARGETING COMPLEXES AND FORMULATIONS FOR TREATING MYOTONIC DYSTROPHY," filed April 15, 2022; the contents of which are incorporated herein by reference in their entirety.

[0002] FIELD OF THE PRESENT APPLICATION The present application relates to targeted complexes for delivering oligonucleotide molecular payloads to cells, formulations containing such complexes, and uses thereof, particularly for the treatment of disease.

[0003] Electronic Sequence Listing Reference The contents of the electronic sequence listing (D082470076WO00-SEQ-COB.xml; size: 56,346 bytes; and creation date: March 22, 2023) are incorporated herein by reference in their entirety. [Background technology]

[0004] background Myotonic dystrophy (DM) is a dominant genetic disease characterized by muscle tone, muscle loss or degeneration, reduced muscle function, insulin resistance, cardiac arrhythmias, smooth muscle dysfunction, and neurological abnormalities. DM is the most common form of adult-onset muscular dystrophy, with a global incidence of approximately 1 in 8000 people worldwide. Two types of the disease have been described: myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2). DM1, the more common form of the disease, results from a repeat expansion of a CTG trinucleotide repeat in the 3' noncoding region of DMPK on chromosome 19; DM2 results from a repeat expansion of a CCTG tetranucleotide repeat in the first intron of ZNF9 on chromosome 3. Repeat expansions of CTG trinucleotide repeats in DM1 patients, which may contain more than about 50 to about 3,000 or more total repeats, lead to the generation of toxic RNA repeats that can form hairpin structures that bind with high affinity to essential intracellular proteins (e.g., muscleblind-like protein), resulting in protein sequestration and the loss-of-function phenotype characteristic of the disease. Apart from supportive care and treatments to address the symptoms of the disease, no effective therapeutic for DM1 is currently available. Summary of the Invention

[0005] overview According to some aspects, the present disclosure provides conjugates and formulations comprising such conjugates.

[0006] According to some aspects, the formulations provided herein include a conjugate comprising an oligonucleotide covalently linked to an anti-transferrin receptor 1 (TfR1) antibody, wherein the anti-TfR1 antibody comprises: a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12; a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13; a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15; a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11; and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16; wherein the oligonucleotide comprises a 5'-XYZ-3' configuration, where X and Z are flanking regions comprising one or more modified nucleosides, and Y is a gap region comprising one or more 2'-deoxyribonucleosides; and where the complex is formulated with tris(hydroxymethyl)aminomethane and sucrose.

[0007] According to some aspects, the formulations provided herein comprise a compound having a structure represented by formula (I): 1 ] n1 -R 2 wherein each R 1 are independently represented by formula (Ia): [ka] In the formula R 2 comprises an antibody, and R 3 comprises an oligonucleotide comprising a 5'-XYZ-3' configuration, where X and Z are flanking regions comprising one or more modified nucleosides and Y is a gap region comprising one or more 2'-deoxyribonucleosides; R in the formula 1 is R 2 is covalently linked to at attachment point A; and In the formula, n1 is R 1where R is an integer representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of the antibody; wherein the conjugate is formulated with tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, each different amino acid residue of the antibody is lysine. In some embodiments, the antibody is an anti-TfR1 antibody.

[0008] In some embodiments, the antibody comprises heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14, light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16.

[0009] In some embodiments, the formulation is in a lyophilized or frozen solid form.

[0010] In some embodiments, the formulation is in an aqueous solution.

[0011] In some embodiments, tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration ranging from 5 mM to 50 mM.

[0012] In some embodiments, sucrose is present in the aqueous solution at a concentration ranging from 5% to 15% weight per volume (w / v %).

[0013] In some embodiments, the aqueous solution has a pH in the range of 6.5 to 8.5.

[0014] In some embodiments, tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration of 25 mM, and / or sucrose is present in the aqueous solution at a concentration of 10 w / v %, and / or the aqueous solution is at a pH of 7.5.

[0015] In some embodiments, the antibody is a Fab fragment, a full length IgG, a Fab' fragment, a F(ab')2 fragment, an scFv, or an Fv.

[0016] In some embodiments, the antibody is a Fab fragment.

[0017] In some embodiments, the antibody comprises a heavy chain variable region (VH) comprising an amino acid sequence at least 85% identical to SEQ ID NO: 17; and / or wherein the antibody comprises a light chain variable region (VL) comprising an amino acid sequence at least 85% identical to SEQ ID NO: 18. In some embodiments, the antibody comprises a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18.

[0018] In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence at least 85% identical to SEQ ID NO: 19; and / or wherein the antibody comprises a light chain comprising an amino acid sequence at least 85% identical to SEQ ID NO: 20. In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20.

[0019] In some embodiments, the oligonucleotide is 10-30 nucleotides in length. In some embodiments, the oligonucleotide comprises a nucleotide sequence having a region of complementarity to SEQ ID NO:22 that is at least 8 consecutive nucleotides in length.

[0020] In some embodiments, the oligonucleotide comprises at least 8 contiguous nucleotides of the nucleotide sequence as set forth in SEQ ID NO: 21. In some embodiments, the oligonucleotide comprises the nucleotide sequence of SEQ ID NO: 21.

[0021] In some embodiments, each R 1 is represented by formula (Ib): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage, such that the oligonucleotide comprises the nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21).

[0022] In some embodiments, each R 1 is represented by the formula (Ic): [ka] The group represented by the formula:

[0023] In some embodiments, the complex is present in the formulation at a concentration ranging from 10 mg / mL to 50 mg / mL.

[0024] In some embodiments, the formulation further comprises one or more antibodies that are not covalently linked to the oligonucleotide.

[0025] In some embodiments, the average value of n1 of the complexes in the formulation is in the range of 0.5-5.

[0026] According to some aspects, provided herein is a method of reducing DMPK expression and / or treating myotonic dystrophy in a subject, the method comprising administering to the subject an effective amount of a formulation provided herein.

[0027] In some embodiments, the subject has a disease-associated repeat expansion of a DMPK allele associated with myotonic dystrophy. In some embodiments, the disease-associated repeat comprises repeating units of the CTG trinucleotide sequence.

[0028] In some embodiments, the complex reduces DMPK expression in a subject. [Brief description of the drawings]

[0029] BRIEF DESCRIPTION OF THE DRAWINGS [Figure 1] Figures 1A-1D show the amount of DMPK-targeted oligonucleotide (ASO) in the heart (Figure 1A), diaphragm (Figure 1B), gastrocnemius muscle (Figure 1C), or tibialis anterior muscle (Figure 1D), respectively, after administration of a conjugate containing an anti-TfR1 Fab covalently linked to the ASO.

[0030] [Diagram 2] Figures 2A-2D show the ability of a conjugate containing an anti-TfR1 Fab conjugated to a DMPK-targeting oligonucleotide (ASO) to knockdown human DMPK RNA in the heart (Figure 2A), diaphragm (Figure 2B), tibialis anterior (Figure 2C), and gastrocnemius (Figure 2D) muscles of mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat.

[0031] [Diagram 3]Figures 3A-3B show reduced DMPK foci in the nuclei of cardiac muscle fibers in mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat and treated with anti-TfR1 Fab conjugated to a DMPK-targeting oligonucleotide (ASO). Figure 3A shows a representative image of a sample after in situ hybridization staining for DMPK foci and fluorescent staining of myofibrils (insert panel). In the microscopic image shown in Figure 3A, the bright round shapes indicate cell nuclei and the bright puncta within the nucleus indicate DMPK foci. Figure 3B shows the quantification of DMPK foci.

[0032] [Figure 4] 4 shows the splicing correction activity of a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) in the hearts of mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat (hTfR1 / DMSXL mice). A composite splicing index based on splicing of Ldb3 exon 11, Mbnl2 exon 6, and Nfix exon 7 is shown for control mice treated with vehicle control ("hTfR1-PBS"), hTfR1 / DMSXL mice treated with vehicle control ("hTfR1 / DMSXL-PBS"), and hTfR1 / DMSXL mice treated with anti-TfR1 Fab-ASO conjugate ("hTfR1 / DMSXL-conjugate").

[0033] [Diagram 5]5 shows the splicing correction activity of a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) in the diaphragm of mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat (hTfR1 / DMSXL mice). A composite splicing index based on the splicing of Bin1 exon 11, Insr exon 11, Ldb3 exon 11, and Nfix exon 7 is shown for control mice treated with vehicle control ("hTfR1-PBS"), hTfR1 / DMSXL mice treated with vehicle control ("hTfR1 / DMSXL-PBS"), and hTfR1 / DMSXL mice treated with anti-TfR1 Fab-ASO conjugate ("hTfR1 / DMSXL-conjugate").

[0034] [Figure 6] 6 shows the splicing correction activity of a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) in the tibialis anterior muscle of mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat (hTfR1 / DMSXL mice). A composite splicing index based on the splicing of Bin1 exon 11, Ldb3 exon 11, Mbnl2 exon 6, and Nfix exon 7 is shown for control mice treated with vehicle control ("hTfR1-PBS"), hTfR1 / DMSXL mice treated with vehicle control ("hTfR1 / DMSXL-PBS"), and hTfR1 / DMSXL mice treated with anti-TfR1 Fab-ASO conjugate ("hTfR1 / DMSXL-conjugate").

[0035] [Figure 7]7 shows the splicing correction activity of a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) in the gastrocnemius muscle of mice expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CTG repeat (hTfR1 / DMSXL mice). A composite splicing index based on the splicing of Mbnl2 exon 6, Nfix exon 7, and Ttn exon 313 is shown for control mice treated with vehicle control ("hTfR1-PBS"), hTfR1 / DMSXL mice treated with vehicle control ("hTfR1 / DMSXL-PBS"), and hTfR1 / DMSXL mice treated with anti-TfR1 Fab-ASO conjugate ("hTfR1 / DMSXL-conjugate").

[0036] [Figure 8] Figure 8 shows DMPK knockdown in DM1 patient myotubes and wild-type non-human primate (NHP) myotubes following incubation with a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO). Results are shown normalized to expression in DM1 patient myotubes or NHP myotubes treated with vehicle alone. Data are shown as mean + standard deviation for n=4 replicates per condition. Statistics were calculated by one-way ANOVA (*, P<0.05; **, P<0.01).

[0037] [Figure 9] FIG. 9 shows the X-ray diffraction (XRD) pattern of a lyophilized preparation of an anti-TfR1 Fab-ASO conjugate.

[0038] [Figure 10-1]Figures 10A-10C show protein concentrations calculated from absorbance data of lyophilized formulations of anti-TfR1 Fab-ASO conjugates after reconstitution at 0 weeks (T0), 4 weeks (T4w), and 8 weeks (T8w) at 2-8°C. Figure 10A shows the concentration of the Fab of the conjugate in mg / ml. Figure 10B shows the concentration of the Fab of the conjugate in μM. [Figure 10-2] FIG. 10C shows the concentration of the oligonucleotides in the conjugates in μM.

[0039] [Figure 11] Figures 11A-11B show the mean percent recovery (Figure 11A) and drug-antibody ratio (DAR; Figure 11B) for lyophilized preparations of anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T4w, and T8w at 2-8°C.

[0040] [Figure 12-1] Figures 12A-12C show protein concentrations calculated from absorbance data of lyophilized formulations of anti-TfR1 Fab-ASO conjugates after reconstitution at 0 weeks (T0), 2 weeks (T2w), 4 weeks (T4w), and 8 weeks (T8w) at 25°C. Figure 12A shows the concentration of the Fab of the conjugate in mg / ml. Figure 12B shows the concentration of the Fab of the conjugate in μM. [Figure 12-2] FIG. 12C shows the concentration of the oligonucleotides in the conjugates in μM.

[0041] [Figure 13] Figures 13A-13B show the mean percent recovery (Figure 13A) and drug-antibody ratio (DAR; Figure 13B) for lyophilized preparations of anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T2w, T4w, and T8w at 25°C.

[0042] [Figure 14-1]Figures 14A-14C show protein concentrations calculated from absorbance data of lyophilized formulations of anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T2w, T4w, and T8w at 40°C. Figure 14A shows the concentration of the Fab of the conjugate in mg / ml. Figure 14B shows the concentration of the Fab of the conjugate in μM. [Figure 14-2] FIG. 14C shows the concentration of the oligonucleotides in the conjugates in μM.

[0043] [Figure 15] Figures 15A-15B show the mean percent recovery (Figure 15A) and drug-antibody ratio (DAR; Figure 15B) for lyophilized anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T2w, T4w, and T8w at 40°C.

[0044] [Figure 16] Figures 16A-16B show the relative area sums of peaks 1 and 2 (Figure 16A) and peaks 3 and 4 (Figure 16B) measured using size exclusion chromatography (SEC) of lyophilized anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T4w, and T8w at 2-8°C.

[0045] [Figure 17] Figures 17A-17B show the relative area sums of peaks 1 and 2 (Figure 17A) and peaks 3 and 4 (Figure 17A) measured using size exclusion chromatography (SEC) of lyophilized anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T2w, T4w, and T8w at 25°C.

[0046] [Figure 18] Figures 18A-18B show the relative area sums of peaks 1 and 2 (Figure 18A) and peaks 3 and 4 (Figure 18A) measured using size exclusion chromatography (SEC) of lyophilized anti-TfR1 Fab-ASO conjugates after reconstitution at T0, T2w, T4w, and T8w at 40°C.

[0047] [Figure 19] FIG. 19 shows the percent purity of the main peak of lyophilized anti-TfR1 Fab-ASO conjugate after reconstitution measured using capillary electrophoresis (CE-SDS) at 2-8° C. and at T0, T4w, and T8w.

[0048] [Figure 20] FIG. 20 shows the percent purity of the main peak of lyophilized anti-TfR1 Fab-ASO conjugate after reconstitution measured using CE-SDS at T0, T2w, T4w, and T8w at 25° C.

[0049] [Figure 21] FIG. 21 shows the percent purity of the main peak of lyophilized anti-TfR1 Fab-ASO conjugate after reconstitution measured using CE-SDS at T0, T2w, T4w, and T8w at 40° C. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0050] Detailed Description of the Invention According to some aspects, the disclosure provides a complex and a formulation comprising such a complex. In some embodiments, the complex is formulated with tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the complex is formulated as an aqueous or lyophilized (e.g., lyophilized powder) form. In some embodiments, the complex comprises an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration) covalently linked to an antibody. In some embodiments, the complex comprises a muscle-targeting complex comprising an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration) covalently linked to an anti-transferrin receptor 1 (TfR1) antibody. In some embodiments, the complex comprises a muscle-targeting complex comprising an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration) covalently linked to an anti-transferrin receptor 1 (TfR1) antibody provided in Table 2. Also provided are methods of using the complexes and formulations described herein to treat a subject having myotonic dystrophy (e.g., DM1) and / or methods of reducing expression or activity of DMPK (e.g., DMPK RNA) in a cell.

[0051] Further aspects of the disclosure, including descriptions of defined terms, are provided below.

[0052] definition Administering: As used herein, the term "administering" or "administration" means providing a conjugate to a subject in a manner that is physiologically and / or (for example and) pharmacologically (for example, to treat a disease in a subject) useful.

[0053] Approximately: As used herein, the term "approximately" or "about" when applied to one or more values ​​of interest refers to a value similar to the stated reference value. In some embodiments, the term "approximately" or "about" refers to a broad range of values ​​that fall within plus or minus (greater or less than) 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, or less of the stated reference value, unless otherwise stated or clear from the context (except where such number would exceed 100% of a feasible value).

[0054] Antibody: As used herein, the term "antibody" refers to a polypeptide that includes at least one immunoglobulin variable domain or at least one antigenic determinant, e.g., a paratope, that specifically binds to an antigen. In some embodiments, the antibody is a full-length antibody. In some embodiments, the antibody is a chimeric antibody. In some embodiments, the antibody is a humanized antibody. However, in some embodiments, the antibody is a Fab fragment, a Fab' fragment, a F(ab')2 fragment, an Fv fragment, or an scFv fragment. In some embodiments, the antibody is a nanobody derived from a camelid antibody, or a nanobody derived from a shark antibody. In some embodiments, the antibody is a diabody. In some embodiments, the antibody comprises a framework with human germline sequences. In another embodiment, the antibody comprises a heavy chain constant region selected from the group consisting of the constant regions of IgG, IgG1, IgG2, IgG2A, IgG2B, IgG2C, IgG3, IgG4, IgA1, IgA2, IgD, IgM, and IgE. In some embodiments, the antibody comprises a heavy (H) chain variable region (abbreviated herein as VH) and / or (for example and) a light (L) chain variable region (abbreviated herein as VL). In some embodiments, the antibody comprises a constant region, for example an Fc region. An immunoglobulin constant region refers to the constant region of a heavy or light chain. The constant region amino acid sequences of human IgG heavy and light chains and their functional variations are known. With respect to the heavy chain, in some embodiments, the heavy chain of the antibody described herein can be an alpha (α), delta (Δ), epsilon (ε), gamma (γ), or mu (μ) heavy chain. In some embodiments, the heavy chain of the antibody described herein can comprise a human alpha (α), delta (Δ), epsilon (ε), gamma (γ), or mu (μ) heavy chain. In specific embodiments, the antibody described herein comprises a human gamma 1 CH1 domain, CH2 domain, and / or (for example and) CH3 domain. In some embodiments, the amino acid sequence of the VH domain comprises the amino acid sequence of a human gamma (γ) heavy chain constant region, such as any known in the art.Non-limiting examples of human constant region sequences are described in the art, see, for example, U.S. Patent No. 5,693,780 and Kabat EA et al., (1991), supra. In some embodiments, the VH domain comprises an amino acid sequence that is at least 70%, 75%, 80%, 85%, 90%, 95%, 98% or at least 99% identical to any of the variable chain constant regions provided herein. In some embodiments, the antibody is modified, for example, via glycosylation, phosphorylation, sumoylation, and / or (for example and) methylation. In some embodiments, the antibody is a glycosylated antibody conjugated to one or more sugar or carbohydrate molecules. In some embodiments, one or more sugar or carbohydrate molecules are conjugated to the antibody via N-glycosylation, O-glycosylation, C-glycosylation, glypiation (GPI anchor attachment), and / or (for example and) phosphoglycosylation. In some embodiments, the one or more sugar or carbohydrate molecules are monosaccharides, disaccharides, oligosaccharides, or glycans. In some embodiments, the one or more sugar or carbohydrate molecules are branched oligosaccharides or branched glycans. In some embodiments, the one or more sugar or carbohydrate molecules include mannose units, glucose units, N-acetylglucosamine units, N-acetylgalactosamine units, galactose units, fucose units, or phospholipid units. In some embodiments, the antibody is a construct comprising a linker polypeptide or a polypeptide comprising one or more antigen-binding fragments of the present disclosure linked to an immunoglobulin constant region. The linker polypeptide comprises two or more amino acid residues linked together by peptide bonds and is used to link one or more antigen-binding moieties. Examples of linker polypeptides have been reported (see, for example, Holliger, P., et al. (1993) Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak, RJ, et al. (1994) Structure 2:1121-1123).Still further, an antibody may be part of a larger immunoadhesion molecule formed by covalent or non-covalent association of the antibody or antibody portion with one or more other proteins or peptides. Examples of such immunoadhesion molecules include the use of streptavidin core regions to generate tetrameric scFv molecules (Kipriyanov, SM, et al. (1995) Human Antibodies and Hybridomas 6:93-101), and the use of cysteine ​​residues, marker peptides, and C-terminal polyhistidine tags to generate bivalent and biotinylated scFv molecules (Kipriyanov, SM, et al. (1994) Mol. Immunol. 31:1047-1058).

[0055] CDR: As used herein, the term "CDR" refers to a complementarity determining region within an antibody variable sequence. A typical antibody molecule contains a heavy chain variable region (VH) and a light chain variable region (VL), which are mostly involved in antigen binding. The VH and VL regions can be further subdivided into regions of hypervariability, also known as "complementarity determining regions" ("CDRs"), interspersed with more conserved regions known as "framework regions" ("FRs"). Each VH and VL is typically composed of three CDRs and four FRs arranged from amino terminus to carboxy terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The extent of the framework regions and CDRs can be precisely identified using methodologies known in the art, for example, by the Kabat definition, the IMGT definition, the Chothia definition, the AbM definition, and / or (for example) the contact definition, all of which are well known in the art.For example, Kabat, EA, et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, USDepartment of Health and Human Services, NIH Publication No. 91-3242; IMGT (registered trademark), the international ImMunoGeneTics information system (registered trademark) http: / / www.imgt.org, Lefranc, M.-P. et al., Nucleic Acids. Res.,27:209-212(1999);Ruiz,M.et al.,Nucleic Acids Res.,28:219-221(2000);Lefranc,M.-P.,Nucleic Acids Res.,29:207-209(2001);Lefranc,M.-P.,Nucleic Acids Res.,31:307-310(2003);Lefranc,M.-P.et al.,In Silico Biol.,5,0006(2004)[Epub],5:45-60(2005);Lefranc,M.-P.et al.,Nucleic Acids Res.,33:D593-597(2005);Lefranc,M.-P.et al.,Nucleic Acids Res.,37:D1006-1012(2009);Lefranc,M.-P.et al.,Nucleic Acids Res.,43:D413-422(2015);Chothia et al.,(1989)Nature 342:877;Chothia,C.et al. (1987) J. Mol. Biol. 196:901-917, Al-lazikani et al. al (1997) J. Molec. Biol. 273:927-948; and Almagro, J. Mol. Recognit. 17:132-143 (2004). See also hgmp.mrc.ac.uk and bioinf.org.uk / abs. As used herein, CDRs may refer to CDRs defined by any method known in the art.Two antibodies having the same CDR means that the two antibodies have the same amino acid sequence of that CDR when determined by the same method, for example, the IMGT definition.

[0056] There are three CDRs in each of the variable regions of heavy and light chains, which are designated as CDR1, CDR2, and CDR3 for each of the variable regions. The term "CDR set" as used herein refers to a group of three CDRs that occur in a single variable region that can bind to antigen. The exact boundaries of these CDRs are defined differently according to various systems. The system described by Kabat (Kabat et al., Sequence of Proteins of Immunological Interest, National Institutes of Health, Bethesda, Md. (1987) and (1991)) not only provides an unambiguous residue numbering system applicable to any variable region of an antibody, but also provides precise residue boundaries defining the three CDRs. These CDRs are sometimes referred to as Kabat CDRs. Sub-portions of the CDRs are sometimes designated L1, L2, and L3, or H1, H2, and H3, where "L" and "H" designate the light chain and heavy chain regions, respectively. These regions are sometimes referred to as Chothia CDRs, which have boundaries that overlap with the Kabat CDRs. Other boundaries defining CDRs that overlap with the Kabat CDRs are described by Padlan (FASEB J. 9:133-139 (1995)) and MacCallum (J Mol Biol 262(5):732-45(1996)). Still other CDR boundary definitions may not strictly adhere to one of the above systems, but may still overlap with the Kabat CDRs, and may be shortened or extended in light of predictions or experimental findings that specific residues or groups of residues, or even entire CDRs, do not significantly affect antigen binding. The methods used herein may utilize CDRs defined according to any of these systems. Examples of CDR definition systems are provided in Table 1. [Table 1]

[0057] Complementary: As used herein, the term "complementary" refers to the ability for precise pairing between two nucleotides or two sets of nucleotides. In particular, complementary is a term that characterizes the degree of hydrogen bond pairing that results in binding between two nucleotides or two sets of nucleotides. For example, if a base at a position of an oligonucleotide can hydrogen bond with a base at a corresponding position of a target nucleic acid (e.g., mRNA), then the bases are considered to be complementary to each other at that position. Base pairing may include both canonical Watson-Crick base pairing and non-Watson-Crick base pairing (e.g., Wobble base pairing and Hoogsteen base pairing). For example, in some embodiments, for complementary base pairing, an adenosine-based base (A) is complementary to a thymidine-based base (T) or a uracil-based base (U), a cytosine-based base (C) is complementary to a guanosine-based base (G), and a universal base such as 3-nitropyrrole or 5-nitroindole can hybridize to and is considered complementary to any A, C, U, or T. Inosine (I) is also considered a universal base in the art and is considered complementary to any A, C, U, or T.

[0058] Covalently linked: As used herein, the term "covalently linked" refers to the characteristic of two or more molecules being linked together through at least one covalent bond. In some embodiments, the two molecules may be covalently linked together by a single bond (e.g., a disulfide bond or a disulfide bridge) that acts as an intermolecular linker. However, in some embodiments, the two or more molecules may be covalently linked together through a molecule that acts as a linker that connects the two or more molecules together through multiple covalent bonds. In some embodiments, the linker may be a cleavable linker. However, in some embodiments, the linker may be a non-cleavable linker.

[0059] Disease-associated repeats: As used herein, the term "disease-associated repeats" refers to repeated nucleotide sequences at genomic locations where the number of units of the repeated nucleotide sequence correlates with and / or (by way of example) directly or indirectly contributes to or causes a genetic disease. Each repeat unit of a disease-associated repeat may be 2, 3, 4, 5, or more nucleotides in length. For example, in some embodiments, the disease-associated repeat is a dinucleotide repeat. In some embodiments, the disease-associated repeat is a trinucleotide repeat. In some embodiments, the disease-associated repeat is a tetranucleotide repeat. In some embodiments, the disease-associated repeat is a pentanucleotide repeat. In some embodiments, the disease-associated repeat comprises a CAG repeat, a CTG repeat, a CUG repeat, a CGG repeat, a CCTG repeat, or the nucleotide complement of any thereof. In some embodiments, the disease-associated repeat is in a non-coding portion of a gene. However, in some embodiments, the disease-associated repeat is in a coding region of a gene. In some embodiments, the disease-associated repeat is extended from a normal state to a length that directly or indirectly contributes to or causes a genetic disease. In some embodiments, the disease-associated repeats are in RNA (e.g., RNA transcripts). In some embodiments, the disease-associated repeats are in DNA (e.g., chromosomes, plasmids). In some embodiments, the disease-associated repeats are expanded in a chromosome of a germline cell. In some embodiments, the disease-associated repeats are expanded in a chromosome of a somatic cell. In some embodiments, the disease-associated repeats are expanded to a number of repeat units associated with congenital onset of the disease. In some embodiments, the disease-associated repeats are expanded to a number of repeat units associated with childhood onset of the disease. In some embodiments, the disease-associated repeats are expanded to a number of repeat units associated with adult onset of the disease. In DM1, the DMPK gene comprises a disease-associated repeat of a CTG unit.

[0060] DMPK: As used herein, the term "DMPK" refers to the gene encoding myotonin protein kinase (also known as myotonic dystrophy protein kinase or myotonic dystrophy protein kinase), a serine / threonine protein kinase. Substrates of this enzyme may include myogenin, beta-subunit of L-type calcium channel, and phospholemman. In some embodiments, DMPK may be a human gene (Gene ID:1760), a non-human primate gene (e.g., Gene ID:456139, Gene ID:715328, Gene ID:102125829), or a rodent gene (e.g., Gene ID:13400). In humans, a CTG repeat expansion in the 3' non-coding untranslated region of DMPK is associated with myotonic dystrophy type I (DM1). In addition, multiple human transcript variants (e.g., as annotated under GenBank RefSeq accession numbers: NM_001081563.2, NM_004409.4, NM_001081560.2, NM_001081562.2, NM_001288764.1, NM_001288765.1, and NM_001288766.1) have been characterized that encode various protein isoforms.

[0061] DMPK allele: As used herein, the term "DMPK allele" refers to any one of the alternative forms (e.g., wild-type or mutant forms) of the DMPK gene. In some embodiments, the DMPK allele may encode a wild-type myotonin protein kinase that retains its normal and typical function. In some embodiments, the DMPK allele may contain one or more disease-associated repeat expansions. In some embodiments, a normal subject has two DMPK alleles with repeat units ranging from 5 to 37. In some embodiments, the number of CTG repeat units in subjects with DM1 ranges from about 50 to about 3,000 or more, with higher repeat numbers being associated with increased disease severity. In some embodiments, a mildly affected DM1 subject has at least one DMPK allele with repeat units ranging from 50 to 150. In some embodiments, a subject with classical DM1 has at least one DMPK allele with repeat units ranging from 100 to 1,000 or more. In some embodiments, subjects who have DM1 and are congenitally affected may have at least one DMPK allele that contains more than 2,000 repeat units.

[0062] Framework: As used herein, the term "framework" or "framework sequence" refers to the remaining sequence of the variable region minus the CDRs. Since the exact definition of the CDR sequence can be determined by different systems, the meaning of the framework sequence is subject to correspondingly different interpretations. The six CDRs (CDR-L1, CDR-L2, and CDR-L3 of the light chain, and CDR-H1, CDR-H2, and CDR-H3 of the heavy chain) also divide the framework region on the light chain and the heavy chain into four subregions (FR1, FR2, FR3, and FR4) on each chain, where CDR1 is located between FR1 and FR2, CDR2 is located between FR2 and FR3, and CDR3 is located between FR3 and FR4. The framework region as referred to by others, without specifying the specific subregion as FR1, FR2, FR3, or FR4, represents the FR(s) combined in the variable region of a naturally occurring single immunoglobulin chain. As used herein, FR refers to one of the four subregions, and FR refers to two or more of the four subregions that contain framework regions. Acceptor sequences for human heavy and light chains are known in the art. In one embodiment, acceptor sequences known in the art may be used in the antibodies disclosed herein.

[0063] Human antibody: The term "human antibody" as used herein is intended to include antibodies having variable and constant regions derived from human germline immunoglobulin sequences. The human antibodies of the present disclosure may include amino acid residues (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo), e.g., in the CDRs, particularly in CDR3, that are not encoded by human germline immunoglobulin sequences. However, the term "human antibody" as used herein is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, are grafted onto human framework sequences.

[0064] Humanized antibody: The term "humanized antibody" refers to an antibody that contains heavy and light chain variable region sequences from a non-human species (e.g., mouse), but in which at least a portion of the VH and / or (e.g., and) VL sequences have been altered to be more "human-like", i.e., more similar to human germline variable sequences. One type of humanized antibody is a CDR-grafted antibody in which human CDR sequences are introduced onto non-human VH and VL sequences, thereby replacing the corresponding non-human CDR sequences. In one embodiment, humanized anti-transferrin receptor antibodies and antigen-binding portions are provided. Such antibodies may be produced by using existing hybridoma technology followed by humanization using in vitro genetic engineering, such as that disclosed in PCT Publication No. WO 2005 / 123126 A2 to Kasaian et al., to obtain a mouse anti-transferrin receptor monoclonal antibody.

[0065] Kabat numbering: The terms "Kabat numbering", "Kabat definition" and "Kabat labeling" are used interchangeably herein. These terms are recognized in the art and refer to a system of numbering amino acid residues that are more variable (i.e., more variable) than other amino acid residues in the variable regions of the heavy and light chains of an antibody or its antigen-binding portion (Kabat et al. (1971) Ann. NY Acad, Sci. 190: 382-391 and Kabat, EA, et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, USDepartment of Health and Human Services, NIH Publication No. 91-3242). For the heavy chain variable region, the hypervariable region extends from amino acid position 31 to 35 for CDR1, from amino acid position 50 to 65 for CDR2, and from amino acid position 95 to 102 for CDR3. For the light chain variable region, the hypervariable region ranges from amino acid positions 24 to 34 for CDR1, from amino acid positions 50 to 56 for CDR2, and from amino acid positions 89 to 97 for CDR3.

[0066] Myotonic dystrophy (DM): As used herein, the term "myotonic dystrophy (DM)" refers to a genetic disease caused by mutations in the DMPK gene or the CNBP (ZNF9) gene, characterized by muscle loss, muscle weakness, and muscle function. Two types of the disease have been described: myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2). DM1 is associated with an expansion of a CTG trinucleotide repeat in the 3' non-coding region of DMPK. DM2 is associated with an expansion of a CCTG tetranucleotide repeat in the first intron of ZNF9. In both DM1 and DM2, the nucleotide expansion leads to a toxic RNA repeat that can form a hairpin structure that binds with high affinity to critical intracellular proteins, such as muscleblind-like protein. Myotonic dystrophy, the genetic basis of the disease and associated symptoms are described in the art (see, e.g., Thornton, CA, "Myotonic Dystrophy" Neurol Clin. (2014), 32(3):705-719.; and Konieczny et al. "Myotonic dystrophy: candidate small molecule therapeutics" Drug Discovery Today (2017), 22:11). In some embodiments, the subject is born with a variation of DM1, called congenital myotonic dystrophy. Symptoms of congenital myotonic dystrophy are present from birth and include all muscle weakness, breathing problems, clubfoot, developmental delay, and intellectual disability. DM1 is associated with Online Mendelian Inheritance in Man (OMIM) Entry #160900. DM2 is associated with OMIM Entry #602668.

[0067] Oligonucleotide: As used herein, the term "oligonucleotide" refers to an oligomeric nucleic acid compound up to 200 nucleotides in length. Examples of oligonucleotides include, but are not limited to, RNAi oligonucleotides (e.g., siRNA, shRNA), microRNA, gapmers, mixmers, phosphorodiamidate morpholinos, peptide nucleic acids, aptamers, guide nucleic acids (e.g., Cas9 guide RNA), and the like. Oligonucleotides may be single-stranded or double-stranded. In some embodiments, oligonucleotides may contain one or more modified nucleosides (e.g., 2'-O-methyl sugar modifications, purine modifications, or pyrimidine modifications). In some embodiments, oligonucleotides may contain one or more modified internucleoside linkages. In some embodiments, oligonucleotides may contain one or more phosphorothioate linkages, which may be in the Rp or Sp stereochemical conformation.

[0068] Region of complementarity: As used herein, the term "region of complementarity" refers to a nucleotide sequence (e.g., of an oligonucleotide) that is sufficiently complementary to a cognate nucleotide sequence (e.g., of a target nucleic acid) so that the two nucleotide sequences can anneal to each other under physiological conditions (e.g., in a cell). In some embodiments, the region of complementarity is fully complementary to the cognate nucleotide sequence of the target nucleic acid. However, in some embodiments, the region of complementarity is partially complementary (e.g., at least 80%, 90%, 95%, or 99% complementary) to the cognate nucleotide sequence of the target nucleic acid. In some embodiments, the region of complementarity contains 1, 2, 3, or 4 mismatches compared to the cognate nucleotide sequence of the target nucleic acid.

[0069] Specific Binding: As used herein, the term "specific binding" refers to the ability of a molecule to bind to a binding partner with a degree of affinity or avidity that allows the molecule to be used to distinguish the binding partner from an appropriate control in a binding assay or other binding context. The term "specific binding" with respect to an antibody refers to the ability of an antibody to bind to a specific antigen with a degree of affinity or avidity that allows the antibody to distinguish the specific antigen from other antigens (e.g., to a degree that allows preferential targeting to certain cells (e.g., muscle cells) through binding to the antigen, as described herein) compared to an appropriate reference antigen(s). In some embodiments, an antibody has at least about 10% affinity for binding to a target. -4 M, 10 -5 M, 10 -6 M, 10 -7 M, 10 -8 M, 10 -9 M, 10 -10 M, 10 -11 M, 10 -12 M, 10 -13 M or less than this K D In some embodiments, the antibody specifically binds to a transferrin receptor, e.g., an epitope in the apical domain of the transferrin receptor.

[0070] Subject: As used herein, the term "subject" refers to a mammal. In some embodiments, the subject is a non-human animal of the primate order or a rodent. In some embodiments, the subject is a human. In some embodiments, the subject is a patient, e.g., a human patient having or suspected of having a disease. In some embodiments, the subject is a human patient having or suspected of having a disease resulting from a disease-associated repeat expansion (e.g., said expansion in a DMPK allele).

[0071] Transferrin receptor: As used herein, the term "transferrin receptor" (also known as TFRC, CD71, p90, TFR, or TFR1) refers to an internalizing cell surface receptor that binds transferrin to facilitate iron uptake by endocytosis. In some embodiments, the transferrin receptor may be of human (NCBI Gene ID 7037) origin, non-human primate (e.g., NCBI Gene ID 711568 or NCBI Gene ID 102136007) origin, or rodent (e.g., NCBI Gene ID 22042) origin. In addition, multiple human transcript variants (e.g., as annotated under GenBank RefSeq accession numbers: NP_001121620.1, NP_003225.2, NP_001300894.1, and NP_001300895.1) that encode different isoforms of the receptor have been characterized.

[0072] 2'-modified nucleoside: As used herein, the terms "2'-modified nucleoside" and "2'-modified ribonucleoside" are used interchangeably to refer to a nucleoside having a sugar moiety modified at the 2' position. In some embodiments, the 2'-modified nucleoside is a 2'-4' bicyclic nucleoside, in which the 2' and 4' positions of the sugar are bridged (e.g., via a methylene, ethylene, or (S)-constrained ethyl bridge). In some embodiments, the 2'-modified nucleoside is a non-bicyclic 2'-modified nucleoside, in which the 2' position of the sugar moiety is substituted (e.g., via a methylene, ethylene, or (S)-constrained ethyl bridge). Non-limiting examples of 2'-modified nucleosides include the following: 2'-deoxy, 2'-fluoro (2'-F), 2'-O-methyl (2'-O-Me), 2'-O-methoxyethyl (2'-MOE), 2'-O-aminopropyl (2'-O-AP), 2'-O-dimethylaminoethyl (2'-O-DMAOE), 2'-O-dimethylaminopropyl (2'-O-DMAP), 2'-O-dimethylaminoethyloxyethyl (2'-O-DMAEOE), 2'-ON-methylacetamide (2'-O-NMA), locked nucleic acid (LNA, methylene bridged nucleic acid), ethylene bridged nucleic acid (ENA), and (S)-constrained ethyl bridged nucleic acid (cEt). In some embodiments, the 2'-modified nucleosides described herein are high affinity modified nucleotides, and oligonucleotides comprising the 2'-modified nucleosides have increased affinity for target sequences compared to unmodified oligonucleotides. Examples of 2'-modified nucleosides structures are provided below: [ka] The examples are shown with a phosphate group, however, any internucleoside linkage is contemplated between 2'-modified nucleosides.

[0073] Ranges: All ranges provided in this disclosure include the endpoints.

[0074] Complex Provided herein is a conjugate comprising a targeting agent, for example an antibody, covalently linked to an oligonucleotide. In some embodiments, the conjugate comprises a muscle-targeting antibody covalently linked to one or more oligonucleotides. In some embodiments, the oligonucleotide is an antisense oligonucleotide that targets DMPK RNA to reduce DMPK expression or activity (for example, reduce the level of mutant or wild-type DMPK RNA, or the activity of DMPK gene product).

[0075] The conjugates described herein generally include a linker that covalently connects an antibody described herein (e.g., an anti-TfR1 antibody) to an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration). The linker includes at least one covalent bond.

[0076] In some embodiments, the conjugates described herein have the formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 independently includes compounds that include an oligonucleotide (e.g., an oligonucleotide that includes a 5'-XYZ-3' configuration), and R 2 includes an antibody (e.g., an anti-TfR1 antibody), and wherein in each complex, n1 independently represents the number of R 1 In some embodiments, each R 1 In some embodiments, each R 1 In some embodiments, R 2 In some embodiments, each R of the complex comprises an antibody (e.g., an anti-TfR1 antibody) that comprises a heavy chain comprising a heavy chain variable region (VH) and a heavy chain constant region, and a light chain comprising a light chain variable region (VL) and a light chain constant region. 1 are independently 2is covalently linked to a different amino acid residue (e.g., lysine or cysteine) of

[0077] In some embodiments, in each complex, n1 is independently an integer (e.g., 1 or more). In some embodiments, the antibody comprises a sequence as described in Table 2. For example, in some embodiments, the antibody comprises a heavy chain complementarity determining region 1 (CDR-H1) comprising a sequence as described in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising a sequence as described in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising a sequence as described in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising a sequence as described in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising a sequence as described in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising a sequence as described in SEQ ID NO: 6 or 16. In some embodiments, the antibody comprises a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 18. In some embodiments, the antibody comprises a VH comprising an amino acid sequence of SEQ ID NO: 17, and / or a VL comprising an amino acid sequence of SEQ ID NO: 18. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 20. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence of SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence of SEQ ID NO: 20. In some embodiments, the antibody is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. In some embodiments, the antibody is a Fab fragment.

[0078] In some embodiments, the value of n1 for each or any of the conjugates (e.g., any of the conjugates in any of the compositions or formulations disclosed herein) is an integer up to the number of amino acid residues (e.g., the number of lysine residues) of the antibody to which conjugation is desired or targeted. In some embodiments, the value of n1 for each of the conjugates is independently selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, and 27. In some embodiments, the value of n1 for each of the conjugates is independently selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, and 26. In some embodiments, the value of n1 in each conjugate is independently in the range of 1 to 27, 1 to 26, 1 to 10, 1 to 5, or 1 to 3. In some embodiments, the average value of n1 for the conjugates of the composition is in the range of 1 to 5 (e.g., 1 to 5, 1 to 4, 1 to 3, 3 to 5, or 1 to 2). In some embodiments, the compositions described herein comprise a compound represented by formula (I): [R 1 ] n1 -R 2 wherein n1 is 0. In some embodiments, the average value of n1 of the complex of the composition is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, n1 in each complex type is independently selected from R 1n1 is an integer equal to or greater than 1 representing the number of instances of each of the conjugate types of the composition, and different conjugate types within each of the conjugate types are characterized by having different n1 values ​​(e.g., n1 values ​​ranging from 1 to 27, 1 to 26, 1 to 25, 1 to 20, 1 to 15, 1 to 10, 1 to 5, or 1 to 3).

[0079] In some embodiments, a composition is provided that includes a plurality of different conjugates (e.g., a formulation that includes tris(hydroxymethyl)aminomethane and / or sucrose as described herein). In some embodiments, the plurality of different conjugates includes a common targeting agent (e.g., an antibody) and a common oligonucleotide (e.g., an oligonucleotide that includes a 5'-XYZ-3' configuration). In such embodiments, the different conjugate types are characterized by having different numbers of oligonucleotides covalently linked to the antibody. For example, in some embodiments, a compound of formula (I): [R 1 ] n1 -R 2 wherein each R 1 independently includes a compound that includes an oligonucleotide (e.g., a DMPK-targeting oligonucleotide), and R 2 includes an antibody (e.g., an anti-TfR1 antibody), and wherein n1 is R 1n1 is an integer representing the number of instances of, and in that respect different conjugates of the composition may have different n1 values ​​(e.g., n1 values ​​ranging from 1 to 27, 1 to 26, 1 to 10, 1 to 5, or 1 to 3). In some embodiments, n1 in the conjugates of the composition is independently an integer. In some embodiments, the average value of n1 of the complexes of the composition is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, the compositions described herein include complexes in which n1 is 0.

[0080] In some embodiments, the compositions described herein include an antibody that is not conjugated to an oligonucleotide (e.g., a trace amount of an antibody) and an antibody that is conjugated to one or more oligonucleotides. In some embodiments, the antibody that is not conjugated to an oligonucleotide has the formula (I): [R 1 ] n1 -R 2 where n1 is zero. Accordingly, in some embodiments, the composition for administration to a subject in the methods described herein may be a compound having a structure represented by formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 independently comprises a group that comprises an oligonucleotide; R 2 includes an antibody, and n1 independently represents R 1 In some embodiments, the compound represented by formula (I): [R 1 ] n1 -R 2The proportion of compounds containing a structure represented by the formula (I) is less than 10%, less than 5%, less than 1%, less than 0.5%, less than 0.1%, less than 0.05%, or less than 0.01%, compared to all compounds represented by that structure in a composition in which n1 is 1 or more. Thus, in some embodiments, the average value of n1 of the complexes in the compositions disclosed herein is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2).

[0081] In some embodiments, R in the complex 1 Each instance of R is covalently linked to a different amino acid residue of the antibody. 1 The amino acid to which R is covalently linked contains an ε-amino group (e.g., lysine, arginine). However, in some embodiments, R 1 In some embodiments, the amino acid to which R is covalently linked is a cysteine. 1 is directly covalently linked to an amino acid residue of the antibody. 1 is indirectly covalently linked to an amino acid of the antibody, e.g., covalently linked to a glycosylation site on the amino acid. 1 is not covalently linked to amino acid residues in the CDR regions of the antibody.

[0082] In some embodiments, the conjugates provided herein (e.g., in the compositions or formulations described herein) have the formula (I): [R 1 ] n1 -R 2 In the formula R 1 Each instance of independently represents the formula (Ia): [ka] In the formula R 3 includes oligonucleotides, e.g., oligonucleotides comprising the 5'-XYZ-3' configuration; and R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2 comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 includes an antibody that is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. 3 comprises an oligonucleotide comprising the nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO: 21). 3 comprises an oligonucleotide comprising the structure represented by +C*+A*oG*oC*dG*dC*dC*dC*dA*dC*dC*dA*oG*oU*+C*+A (SEQ ID NO:21), where +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-MOE modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage.

[0083] In some embodiments, the conjugates provided herein (e.g., in the compositions or formulations described herein) have the formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 is represented by the formula (Ic): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, * represents a phosphorothioate internucleoside linkage, and wherein the oligonucleotide comprises a nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21), wherein n1 represents the R 1 is an integer (e.g., 1 or greater) representing the number of instances of, and each R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2 comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 includes antibodies that are a Fab fragment, a full length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv.

[0084] In some embodiments, the conjugates provided herein (e.g., in the compositions or formulations described herein) have the formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 is represented by the formula (Ic): [ka] In the formula R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 includes antibodies that are a Fab fragment, a full length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv.

[0085] In some embodiments, the conjugates provided herein (e.g., in compositions or formulations described herein) have the formula (Id): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, * represents a phosphorothioate internucleoside linkage, and wherein the oligonucleotide comprises a nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21); 2 includes an antibody comprising a sequence as set forth in Table 2; where n1 is an integer (e.g., 1 or more) representing the number of instances of the group enclosed by the brackets, where each instance of the group enclosed by the brackets is covalently linked to a different amino acid residue of the antibody, optionally where each different amino acid residue is a lysine. For example, in some embodiments, R 2 comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 includes antibodies that are a Fab fragment, a full length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv.

[0086] In some embodiments, the conjugates described herein have the formula (A): [ka] where y is 0-15 (e.g., 3) and z is 0-15 (e.g., 4). In some embodiments, the amide shown adjacent to an antibody (e.g., an anti-TfR1 antibody) in structure (A) results from reaction with an amine of the antibody, such as a lysine epsilon amine. In some embodiments, the conjugates described herein include an anti-TfR1 antibody (e.g., an anti-TfR1 Fab) covalently linked through a lysine of the antibody to the 5' end of an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration), in some embodiments, the antibody comprises a sequence as set forth in Table 2. For example, in some embodiments, the antibody comprises a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. In some embodiments, the antibody comprises a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. In some embodiments, the antibody comprises a VH comprising the amino acid sequence of SEQ ID NO: 17, and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20.In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. In some embodiments, the antibody is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv.

[0087] antibody In some embodiments, the conjugates described herein comprise an antibody that binds to human transferrin receptor 1 (TfR1). An example of a human TfR1 amino acid sequence, corresponding to the NCBI sequence NP_003225.2 (transferrin receptor protein 1 isoform 1, homo sapiens), is as follows: (Sequence number 23).

[0088] Table 2 provides examples of anti-TfR1 antibody sequences useful in the conjugates provided herein. [Table 2]

[0089] In some embodiments, an anti-TfR1 antibody of the disclosure comprises heavy chain complementarity determining region 1 (CDR-H1) of SEQ ID NO: 1 (according to the IMGT definition system), heavy chain complementarity determining region 2 (CDR-H2) of SEQ ID NO: 2 (according to the IMGT definition system), heavy chain complementarity determining region 3 (CDR-H3) of SEQ ID NO: 3 (according to the IMGT definition system), light chain complementarity determining region 1 (CDR-L1) of SEQ ID NO: 4 (according to the IMGT definition system), light chain complementarity determining region 2 (CDR-L2) of SEQ ID NO: 5 (according to the IMGT definition system), and light chain complementarity determining region 3 (CDR-L3) of SEQ ID NO: 6 (according to the IMGT definition system).

[0090] In some embodiments, an anti-TfR1 antibody of the disclosure comprises heavy chain complementarity determining region 1 (CDR-H1) of SEQ ID NO: 7 (according to the Kabat definition system), heavy chain complementarity determining region 2 (CDR-H2) of SEQ ID NO: 8 (according to the Kabat definition system), heavy chain complementarity determining region 3 (CDR-H3) of SEQ ID NO: 9 (according to the Kabat definition system), light chain complementarity determining region 1 (CDR-L1) of SEQ ID NO: 10 (according to the Kabat definition system), light chain complementarity determining region 2 (CDR-L2) of SEQ ID NO: 11 (according to the Kabat definition system), and light chain complementarity determining region 3 (CDR-L3) of SEQ ID NO: 6 (according to the Kabat definition system).

[0091] In some embodiments, an anti-TfR1 antibody of the disclosure comprises heavy chain complementarity determining region 1 (CDR-H1) of SEQ ID NO: 12 (according to the Chothia definition system), heavy chain complementarity determining region 2 (CDR-H2) of SEQ ID NO: 13 (according to the Chothia definition system), heavy chain complementarity determining region 3 (CDR-H3) of SEQ ID NO: 14 (according to the Chothia definition system), light chain complementarity determining region 1 (CDR-L1) of SEQ ID NO: 15 (according to the Chothia definition system), light chain complementarity determining region 2 (CDR-L2) of SEQ ID NO: 5 (according to the Chothia definition system), and light chain complementarity determining region 3 (CDR-L3) of SEQ ID NO: 16 (according to the Chothia definition system).

[0092] In some embodiments, the anti-TfR1 antibodies of the present disclosure comprise a heavy chain variable region (VH) that contains 25 or fewer amino acid variations (e.g., 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 or fewer amino acid variations) in the framework regions compared to a VH that comprises the amino acid sequence of SEQ ID NO: 17. Alternatively or in addition (e.g., in addition), the anti-TfR1 antibodies of the present disclosure comprise a light chain variable region (VL) that contains 25 or fewer amino acid variations (e.g., 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 or fewer amino acid variations) in the framework regions compared to a VL that comprises the amino acid sequence of SEQ ID NO: 18.

[0093] In some embodiments, an anti-TfR1 antibody of the disclosure comprises a VH comprising an amino acid sequence in a framework region that is at least 75% (e.g., 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to a VH comprising the amino acid sequence of SEQ ID NO: 17. Alternatively or additionally (e.g., in addition), in some embodiments, an anti-TfR1 antibody of the disclosure comprises a VL comprising an amino acid sequence in a framework region that is at least 75% (e.g., 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to a VL comprising the amino acid sequence of SEQ ID NO: 18.

[0094] In some embodiments, an anti-TfR1 antibody of the present disclosure comprises a VH comprising the amino acid sequence of SEQ ID NO: 17. Alternatively or additionally (for example, in addition), in some embodiments, an anti-TfR1 antibody of the present disclosure comprises a VL comprising the amino acid sequence of SEQ ID NO: 18.

[0095] In some embodiments, an anti-TfR1 antibody of the present disclosure comprises a heavy chain comprising an amino acid sequence at least 75% (e.g., 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to the amino acid sequence of SEQ ID NO: 19. Alternatively or additionally (e.g., in addition), an anti-TfR1 antibody of the present disclosure comprises a light chain comprising an amino acid sequence at least 75% (e.g., 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to the amino acid sequence of SEQ ID NO: 20. In some embodiments, an anti-TfR1 antibody of the present disclosure is a Fab comprising a heavy chain comprising an amino acid sequence at least 75% (e.g., 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to the amino acid sequence of SEQ ID NO: 19. Alternatively or additionally (for example, in addition), an anti-TfR1 antibody of the disclosure is a Fab comprising a light chain comprising an amino acid sequence at least 75% (for example, 75%, 80%, 85%, 90%, 95%, 98%, or 99%) identical to the amino acid sequence of SEQ ID NO:20.

[0096] In some embodiments, an anti-TfR1 antibody of the present disclosure comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19. Alternatively or additionally (for example, in addition), an anti-TfR1 antibody of the present disclosure comprises a light chain comprising the amino acid sequence of SEQ ID NO: 20. In some embodiments, an anti-TfR1 antibody of the present disclosure is a Fab comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19. Alternatively or additionally (for example, in addition), an anti-TfR1 antibody of the present disclosure is a Fab comprising a light chain comprising the amino acid sequence of SEQ ID NO: 20.

[0097] In some embodiments, the anti-TfR1 antibodies provided herein may have one or more post-translational modifications. In some embodiments, N-terminal cyclization, also referred to as pyroglutamate formation (pyroGlu), may occur at the N-terminal glutamate (Glu) and / or glutamine (Gln) residues during production in antibodies. Therefore, it should be understood that an antibody identified as having a sequence that includes an N-terminal glutamate or glutamine residue encompasses antibodies that have undergone pyroglutamate formation as a result of post-translational modification. In some embodiments, pyroglutamate formation occurs in the heavy chain sequence. In some embodiments, pyroglutamate formation occurs in the light chain sequence.

[0098] Oligonucleotides In some embodiments, the oligonucleotide of the conjugate described herein is a single stranded oligonucleotide. In some embodiments, the oligonucleotide is useful for targeting DMPK (e.g., for reducing the expression or activity of DMPK RNA, such as the level of mutant or wild type DMPK RNA). In some embodiments, the oligonucleotide is useful for targeting DMPK RNA (e.g., for reducing the expression or activity of DMPK RNA, such as the level of mutant or wild type DMPK RNA). In some embodiments, the oligonucleotide comprises a region of complementarity to DMPK RNA. In some embodiments, the oligonucleotide is useful for reducing the level of toxic DMPK with disease-associated repeat expansion, e.g., in a subject having or suspected of having myotonic dystrophy. In some embodiments, the oligonucleotide is designed to direct RNAse H-mediated degradation of the target DMPK RNA in the nucleus of a cell, e.g., a muscle cell (e.g., a myotube) or a cell of the nervous system (e.g., a central nervous system (CNS) cell). In some embodiments, the oligonucleotide is designed to have desired bioavailability and / or serum stability properties. In some embodiments, the oligonucleotide is designed to have desired binding affinity properties. In some embodiments, the oligonucleotides are designed to have a desired toxicity profile, hi some embodiments, the oligonucleotides are designed to have low complement activation and / or cytokine induction properties.

[0099] In some embodiments, the DMPK-targeting oligonucleotides described herein are designed to trigger RNase H-mediated degradation of DMPK mRNA. It should be understood that in some embodiments, an oligonucleotide in one format (e.g., an antisense oligonucleotide) may be suitably adapted to another format (e.g., an siRNA oligonucleotide) by incorporating a functional sequence (e.g., an antisense strand sequence) from one format into the other.

[0100] Examples of oligonucleotides useful for targeting DMPK include those disclosed in U.S. Patent Application Publication No. 20100016215A1, published on January 1, 2010, entitled Compound And Method For Treating Myotonic Dystrophy; U.S. Patent Application Publication No. 20130237585A1, published on July 19, 2010, entitled Modulation Of Dystrophia Myotonica-Protein Kinase (DMPK) Expression; U.S. Patent Application Publication No. 20150064181A1, published on March 5, 2015, entitled "Antisense Conjugates For Decreasing Expression Of Dmpk"; and U.S. Patent Application Publication No. 20150064181A1, published on August 27, 2015, entitled "Peptide-Linked Morpholino Antisense Oligonucleotides For Treatment Of Myotonic Dystrophy." No. 20150238627A1, entitled "Compounds And Methods For Modulation Of Dystrophia Myotonica-Protein Kinase (Dmpk) Expression," published on October 20, 2016, the contents of each of which are incorporated herein in their entirety.

[0101] In some embodiments, the oligonucleotide may comprise a region of complementarity to the sequence set forth as follows, which is an example of a human DMPK gene sequence (Gene ID 1760; NM_001081560.2):

[0102] In some embodiments, the oligonucleotide may comprise a region of complementarity to the sequence set forth as follows, which is an example of a mouse DMPK gene sequence (Gene ID 13400; NM_001190490.1):

[0103] In some embodiments, the oligonucleotide may contain a region of complementarity to a mutant form of DMPK, such as those reported in Botta A. et al. "The CTG repeat expansion size correlates with the splicing defects observed in muscles from myotonic dystrophy type 1 patients" J Med Genet. 2008 Oct; 45(10): 639-46.; and Machuca-Tzili L. et al. "Clinical and molecular aspects of the myotonic dystrophies: a review" Muscle Nerve. 2005 Jul; 32(1): 1-18.; the contents of each of which are incorporated herein by reference in their entirety.

[0104] In some embodiments, the oligonucleotide provided herein is an antisense oligonucleotide targeting DMPK. In some embodiments, the oligonucleotide targeting DMPK is any one of the antisense oligonucleotides targeting DMPK as described in US Patent Application Publication 20160304877A1 entitled "Compounds And Methods For Modulation Of Dystrophia Myotonica-Protein Kinase (DMPK) Expression" published on October 20, 2016, which is incorporated herein by reference. In some embodiments, the DMPK targeting oligonucleotide targets the region of the DMPK gene sequence as described in Genbank Accession No. NM_001081560.2 (SEQ ID NO: 24) or as described in Genbank Accession No. NG_009784.1 (SEQ ID NO: 26).

[0105] In some embodiments, the DMPK-targeting oligonucleotides provided herein comprise a nucleotide sequence that includes a region complementary to a target region that is at least 8 contiguous nucleotides (e.g., at least 8, at least 9, at least 10, at least 12, at least 14, at least 16, at least 18, at least 20, or more contiguous nucleotides) of SEQ ID NO:24.

[0106] In some embodiments, the DMPK-targeting oligonucleotides provided herein are 10-35 (e.g., 10-35, 10-30, 10-25, 10-20, 10-15, 15-35, 15-30, 15-25, 15-20, 20-35, 20-30, 13-18, 14-17, 15-18, 20-30, 15-17, 27-30, 25-35, or 30-35) nucleotides in length. In some embodiments, the DMPK-targeting oligonucleotides provided herein are 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length, optionally 15-30 or 16 nucleotides in length. In some embodiments, the DMPK-targeting oligonucleotides provided herein are 16 nucleotides in length.

[0107] In some embodiments, the DMPK-targeting oligonucleotides provided herein comprise a region of complementarity of at least 8 (e.g., at least 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more) consecutive nucleotides to DMPK RNA.

[0108] In some embodiments, the DMPK-targeting oligonucleotides provided herein comprise a region of complementarity of at least 8 (e.g., at least 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more) consecutive nucleotides to a DMPK sequence as set forth in SEQ ID NO:24 or 25.

[0109] In some embodiments, the DMPK targeting oligonucleotides provided herein comprise a region of complementarity of at least 8 (e.g., at least 8, 9, 10, 11, 12, 13, 14, 15, or 16) consecutive nucleotides to a target sequence as set forth in SEQ ID NO: 22 (TGACTGGTGGGCGCTG). In some embodiments, oligonucleotides useful for targeting DMPK comprise at least 8 (e.g., at least 8, 9, 10, 11, 12, 13, 14, 15, or 16) consecutive nucleotides of a sequence as set forth in SEQ ID NO: 21 (CAGCGCCCACCAGUCA). In some embodiments, oligonucleotides useful for targeting DMPK comprise the nucleotide sequence of SEQ ID NO: 21.

[0110] In some embodiments, the DMPK-targeting oligonucleotide comprises a 5'-XYZ-3' configuration. An oligonucleotide comprising a 5'-XYZ-3' configuration may refer to a chimeric antisense compound in which a gap region having multiple nucleosides that support RNase H cleavage is positioned between flanking regions having one or more nucleotides, where the nucleosides comprising the gap region are chemically distinct from the nucleoside(s) comprising the flanking region. In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) comprise a 5'-XYZ-3' configuration with X and Z as flanking regions around the gap region Y. In some embodiments, the gap region Y comprises one or more 2'-deoxyribonucleosides. In some embodiments, each nucleoside in the gap region Y is a 2'-deoxyribonucleoside, and neither the flanking region X nor the flanking region Z contains any 2'-deoxyribonucleosides.

[0111] In some embodiments, the gap region Y comprises a contiguous stretch of six or more 2'-deoxyribonucleosides capable of recruiting an RNAse, such as RNAse H. In some embodiments, the oligonucleotide binds to a target nucleic acid at which point an RNAse can be recruited and then cleave the target nucleic acid. In some embodiments, the flanking regions X and Z each comprise one or more modified nucleosides. In some embodiments, the flanking regions X and Z each comprise one or more high affinity modified nucleosides, for example, 1-6 high affinity modified nucleosides. Examples of high affinity modified nucleosides include, but are not limited to, 2'-modified nucleosides (for example, 2'-MOE, 2'-O-Me, 2'-F) or 2'-4' bicyclic nucleosides (for example, LNA, cEt, ENA). In some embodiments, the flanking regions X and Z may be 1-20 nucleotides, 1-8 nucleotides, or 1-5 nucleotides in length. Flanking regions X and Z may be of similar or dissimilar lengths. In some embodiments, gap region Y may comprise a nucleotide sequence between 5 and 20 nucleotides, between 5 and 15 nucleotides, between 5 and 12 nucleotides, or between 6 and 10 nucleotides in length.

[0112] In some embodiments, gap region Y comprises one or more unmodified internucleoside linkages. In some embodiments, one or both of flanking regions X and Z each independently comprises a phosphorothioate internucleoside linkage (e.g., a phosphorothioate internucleoside linkage or other linkage) between at least two, at least three, at least four, at least five, or more nucleotides. In some embodiments, gap region Y and the two flanking regions X and Z each independently comprise a modified internucleoside linkage (e.g., a phosphorothioate internucleoside linkage or other linkage) between at least two, at least three, at least four, at least five, or more nucleotides.

[0113] In some embodiments, the gap region Y in the gapmer is 5-20 nucleosides in length. For example, the gap region Y may be 5-20, 5-15, 5-10, 10-20, 10-15, or 15-20 nucleosides in length. In some embodiments, the gap region Y is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleosides in length. In some embodiments, each nucleoside in the gap region Y is a 2'-deoxyribonucleoside. In some embodiments, all nucleosides in the gap region Y are 2'-deoxyribonucleosides. In some embodiments, one or more of the nucleosides in the gap region Y are modified nucleosides (e.g., 2'-modified nucleosides, such as those described herein). In some embodiments, one or more cytosines in gap region Y are optionally 5-methylcytosines. In some embodiments, each cytosine in gap region Y is a 5-methyl-cytosine.

[0114] In some embodiments, the flanking region X of the oligonucleotide (X in the 5'-XYZ-3' arrangement) and the flanking region Z of the oligonucleotide (Z in the 5'-XYZ-3' arrangement) are independently 1-20 nucleosides in length. For example, the flanking region X of the oligonucleotide and the flanking region Z of the oligonucleotide may be independently 1-20, 1-15, 1-10, 1-7, 1-5, 1-3, 1-2, 2-5, 2-7, 3-5, 3-7, 5-20, 5-15, 5-10, 10-20, 10-15, or 15-20 nucleosides in length. In some embodiments, the flanking region X of the oligonucleotide and the flanking region Z of the oligonucleotide are independently 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleosides in length. In some embodiments, the flanking region X of the oligonucleotide and the flanking region Z of the oligonucleotide are of the same length. In some embodiments, the flanking region X of the oligonucleotide and the flanking region Z of the oligonucleotide are of different lengths. In some embodiments, the flanking region X of the oligonucleotide is longer than the flanking region Z of the oligonucleotide. In some embodiments, the flanking region X of the oligonucleotide is shorter than the flanking region Z of the oligonucleotide.

[0115] In some embodiments, the oligonucleotides described herein (e.g., DMPK-targeting oligonucleotides) are 5-10-5, 4-12-4, 3-14-3, 2-16-2, 1-18-1, 3-10-3, 2-10-2, 1-10-1, 2-8-2, 4-6-4, 3-6-3, 2-6-2, 4-7-4, 3-7-3, 2-7-2, 4-8-4, 3-8-3, 2-8-2, 1-8-1, 2-9-2, 1-9-1, 2-10-2, 1-10-1, 1-12-1, 1-16-1, 2-15-1, 1-15-2, 1-14-3, 3-14-1, 2-14-2 , 1-13-4, 4-13-1, 2-13-3, 3-13-2, 1-12-5, 5-12-1, 2-12-4, 4-12-2, 3-12-3, 1-11-6, 6-11-1, 2-11-5, 5-11-2, 3-11-4, 4-11-3, 1-17-1, 2-16-1, 1-16- 2, 1-15-3, 3-15-1, 2-15-2, 1-14-4, 4-14-1, 2-14-3, 3-14-2, 1-13-5, 5-13-1, 2-13-4, 4-13-2, 3-13-3, 1-12-6, 6-12-1, 2-12-5, 5-12-2, 3-12-4, 4-12 -3, 1-11-7, 7-11-1, 2-11-6, 6-11-2, 3-11-5, 5-11-3, 4-11-4, 1-18-1, 1-17-2, 2-17-1, 1-16-3, 1-16-3, 2-16-2, 1-15-4, 4-15-1, 2-15-3, 3-15-2, 1-1 4-5, 5-14-1, 2-14-4, 4-14-2, 3-14-3, 1-13-6, 6-13-1, 2-13-5, 5-13-2, 3-13-4, 4-13-3, 1-12-7, 7-12-1, 2-12-6, 6-12-2, 3-12-5, 5-12-3, 1-11-8, 8- 11-1, 2-11-7, 7-11-2, 3-11-6, 6-11-3, 4-11-5, 5-11-4, 1-18-1, 1-17-2, 2-17-1, 1-16-3, 3-16-1, 2-16-2, 1-15-4, 4-15-1, 2-15-3, 3-15-2, 1-14-5, 2 -14-4, 4-14-2, 3-14-3, 1-13-6, 6-13-1, 2-13-5, 5-13-2, 3-13-4, 4-13-3, 1-12-7, 7-12-1, 2-12-6, 6-12-2, 3-12-5, 5-12-3, 1-11-8, 8-11-1, 2-11-7,7-11-2、3-11-6、6-11-3、4-11-5、5-11-4、1-19-1、1-18-2、2-18-1、1-17-3、3-17-1、2-17-2、1-16-4、4-16-1、2-16-3、3-16-2、1-15-5、2-15-4、4-15-2、3-15-3、1-14-6、6-14-1、2-14-5、5-14-2、3-14-4、4-14-3、1-13-7、7-13-1、2-13-6、6-13-2、3-13-5、5-13-3、4-13-4、1-12-8、8-12-1、2-12-7、7-12-2、3-12-6、6-12-3、4-12-5、5-12-4、2-11-8、8-11-2、3-11-7、7-11-3、4-11-6、6-11-4、5-11-5、1-20-1、1-19-2、2-19-1、1-18-3、3-18-1、2-18-2、1-17-4、4-17-1、2-17-3、3-17-2、1-16-5、2-16-4、4-16-2、3-16-3、1-15-6、6-15-1、2-15-5、5-15-2、3-15-4、4-15-3、1-14-7、7-14-1、2-14-6、6-14-2、3-14-5、5-14-3、4-14-4、1-13-8、8-13-1、2-13-7、7-13-2、3-13-6、6-13-3、4-13-5、5-13-4、2-12-8、8-12-2、3-12-7、7-12-3、4-12-6、6-12-4、5-12-5、3-11-8、8-11-3、4-11-7、7-11-4、5-11-6、6-11-5、1-21-1、1-20-2、2-20-1、1-20-3、3-19-1、2-19-2、1-18-4、4-18-1、2-18-3、3-18-2、1-17-5、2-17-4、4-17-2、3-17-3、1-16-6、6-16-1、2-16-5、5-16-2、3-16-4、4-16-3、1-15-7、7-15-1、2-15-6、6-15-2、3-15-5、5-15-3、4-15-4、1-14-8、8-14-1、2-14-7、7-14-2、3-14-6、6-14-3、4-14-5、5-14-4、2-13-8、8-13-2、3-13-7、7-13-3、4-13-6、6-13-4、5-13-5、1-12-10、10-12-1、2-12-9、9-12-2、3-12-8、8-12-3, 4-12-7, 7-12-4, 5-12-6, 6-12-5, 4-11-8, 8-11-4, 5-11-7, 7-11-5, 6-11-6, 1-22-1, 1-21-2, 2-21-1, 1-21-3, 3-20-1, 2-20-2, 1-19-4, 4-19-1, 2-19-3, 3-19-2, 1-18-5, 2-18-4, 4-18-2, 3-18-3, 1-17-6, 6-17-1, 2-17-5, 5-17-2, 3-17-4, 4-17-3, 1-16-7, 7-16-1, 2-16-6, 6-16-2, 3-16- and 5-15-4, 2-14-8, 8-14-2, 3-14-7, 7-14-3, 4-14-6, 6-14-4, 5-14-5, 3-13-8, 8-13-3, 4-13-7, 7-13-4, 5-13-6, 6-13-5, 4-12-8, 8-12-4, 5-12-7, 7-12-5, 6-12-6, 5-11-8, 8-11-5, 6-11-7, or 7-11-6. The numbers indicate the number of nucleosides in the X, Y, and Z regions, respectively, in an oligonucleotide comprising the 5'-XYZ-3' configuration.

[0116] In some embodiments, one or more nucleosides in the flanking region X of the oligonucleotide (X in a 5'-XYZ-3' arrangement) or the flanking region Z of the oligonucleotide (Z in a 5'-XYZ-3' arrangement) are modified nucleosides (e.g., high affinity modified nucleosides). In some embodiments, the modified nucleoside (e.g., high affinity modified nucleoside) is a 2'-modified nucleoside. In some embodiments, the 2'-modified nucleoside is a 2'-4' bicyclic nucleoside or a non-bicyclic 2' modified nucleoside. In some embodiments, the high affinity modified nucleoside is a 2'-4' bicyclic nucleoside (e.g., LNA, cEt, or ENA) or a non-bicyclic 2'-modified nucleoside (e.g., 2'-fluoro (2'-F), 2'-O-methyl (2'-O-Me), 2'-O-methoxyethyl (2'-MOE), 2'-O-aminopropyl (2'-O-AP), 2'-O-dimethylaminoethyl (2'-O-DMAOE), 2'-O-dimethylaminopropyl (2'-O-DMAP), 2'-O-dimethylaminoethyloxyethyl (2'-O-DMAEOE), or 2'-ON-methylacetamide (2'-O-NMA)).

[0117] In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) comprise a 5'-XYZ-3' configuration, where X and Z are independently 2-7 (e.g., 2, 3, 4, 5, 6, or 7) nucleosides in length, and Y is 6-10 (e.g., 6, 7, 8, 9, or 10) nucleosides in length, where positions 1, 2, 3, 4, 5, 6, or At least one but not all (e.g., 1, 2, 3, 4, 5, or 6) of 7 (the 5'-most position is position 1) is a non-bicyclic 2'-modified nucleoside (e.g., 2'-MOE or 2'-O-Me), where the remainder of the nucleosides in both X and Z are 2'-4' bicyclic nucleosides (e.g., LNA or cEt), and where each nucleoside in Y is a 2' deoxyribonucleoside. In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) comprise a 5'-XYZ-3' configuration, where X and Z are independently 2-7 (e.g., 2, 3, 4, 5, 6, or 7) nucleosides in length, and Y is 6-10 (e.g., 6, 7, 8, 9, or 10) nucleosides in length, where positions 1, 2, 3, 4, 5, 6, or 7 in Z are 5'-XYZ-3'. At least one but not all (e.g., 1, 2, 3, 4, 5, or 6) of 7 (the 5'-most position is position 1) is a non-bicyclic 2'-modified nucleoside (e.g., 2'-MOE or 2'-O-Me), where the remainder of the nucleosides in both X and Z are 2'-4' bicyclic nucleosides (e.g., LNA or cEt), and where each nucleoside in Y is a 2' deoxyribonucleoside.In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) comprise a 5'-XYZ-3' configuration, where X and Z are independently 2-7 (e.g., 2, 3, 4, 5, 6, or 7) nucleosides in length, and Y is 6-10 (e.g., 6, 7, 8, 9, or 10) nucleosides in length, where at least one, but not all (e.g., 1, 2, 3, 4, 5, 6, or 7) of positions 1, 2, 3, 4, 5, 6, or 7 in X is 5'-XYZ-3'. At least one but not all (e.g., 1, 2, 3, 4, 5, or 6) of positions 1, 2, 3, 4, 5, 6, or 7 (the 5'-most position is position 1) in Z is a non-bicyclic 2'-modified nucleoside (e.g., 2'-MOE or 2'-O-Me), where the remainder of the nucleosides in both X and Z are 2'-4' bicyclic nucleosides (e.g., LNA or cEt), and where each nucleoside in Y is a 2' deoxyribonucleoside.

[0118] In some embodiments, the oligonucleotides described herein (e.g., DMPK-targeting oligonucleotides) are 10-20 nucleosides in length (e.g., 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleosides), contain a region of complementarity to at least 8 consecutive nucleosides (e.g., at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, or at least 16 consecutive nucleosides) of SEQ ID NO: 22 (TGACTGGTGGGCGCTG), and contain a 5'-XYZ-3' configuration, where X is 3 to 5 (e.g., 3, 4, or 5) linked nucleosides. X comprises 6 to 10 (e.g., 6, 7, 8, 9, or 10) linked 2'-deoxyribonucleosides, where at least one of the nucleosides in X is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, LNA, cEt, or ENA); Y comprises 6 to 10 (e.g., 6, 7, 8, 9, or 10) linked 2'-deoxyribonucleosides, where each cytosine in Y is optionally and independently a 5-methyl-cytosine; and Z comprises 3 to 5 (e.g., 3, 4, or 5) linked nucleosides, where at least one of the nucleosides in Z is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, LNA, cEt, or ENA).

[0119] In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) comprise at least 8 contiguous nucleosides (e.g., at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, or at least 16 contiguous nucleosides) of the nucleotide sequence of SEQ ID NO:21 (CAGCGCCCACCAGUCA) and comprise a 5'-XYZ-3' configuration, where X comprises 3 to 5 (e.g., 3, 4, or 5) linked nucleosides, and where at least one of the nucleosides in X is a 5'-XYZ-3' arrangement. is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, LNA, cEt, or ENA); Y comprises 6 to 10 (e.g., 6, 7, 8, 9, or 10) linked 2'-deoxyribonucleosides, where each cytosine in Y is optionally and independently a 5-methyl-cytosine; and Z comprises 3 to 5 (e.g., 3, 4, or 5) linked nucleosides, where at least one of the nucleosides in Z is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, LNA, cEt, or ENA). In some embodiments, each thymine base (T) in the nucleotide sequence of the antisense oligonucleotide may be independently and optionally replaced with a uracil base (U), and each U may be independently and optionally replaced with a T.

[0120] In some embodiments, an oligonucleotide described herein (e.g., a DMPK-targeted oligonucleotide described herein) comprises the nucleotide sequence of SEQ ID NO:21 and comprises a 5'-XYZ-3' configuration, where X comprises 3 to 5 (e.g., 3, 4, or 5) linked nucleosides, and where at least one of the nucleosides in X is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, an LNA, a cEt, or an ENA). ); Y comprises 6 to 10 (e.g., 6, 7, 8, 9, or 10) linked 2'-deoxyribonucleosides, where each cytosine in Y is optionally and independently a 5-methyl-cytosine; and Z comprises 3 to 5 (e.g., 3, 4, or 5) linked nucleosides, where at least one of the nucleosides in Z is a 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside, a 2'-O-Me modified nucleoside, LNA, cEt, or ENA). In some embodiments, each thymine base (T) in the nucleotide sequence of the antisense oligonucleotide may be independently and optionally replaced with a uracil base (U), and each U may be independently and optionally replaced with a T.

[0121] In some embodiments, X comprises at least one 2'-4' bicyclic nucleoside (e.g., LNA, cEt, or ENA) and at least one non-bicyclic 2'-modified nucleoside, e.g., a 2'-MOE modified nucleoside or a 2'-O-Me modified nucleoside, and / or (e.g., and) Z comprises at least one 2'-4' bicyclic nucleoside (e.g., LNA, cEt, or ENA) and at least one non-bicyclic 2'-modified nucleoside (e.g., a 2'-MOE modified nucleoside or a 2'-O-Me modified nucleoside).

[0122] In some embodiments, the 2'-4' bicyclic nucleoside is selected from LNA, cEt, and ENA nucleosides. In some embodiments, the non-bicyclic 2'-modified nucleoside is a 2'-MOE modified nucleoside or a 2'-OMe modified nucleoside.

[0123] In some embodiments, the nucleosides of the oligonucleotide are linked together by phosphorothioate internucleoside linkages, phosphodiester internucleoside linkages, or a combination thereof. In some embodiments, the oligonucleotide contains only phosphorothioate internucleoside linkages linking each nucleoside (i.e., the oligonucleotide contains a complete phosphorothioate backbone). In some embodiments, the oligonucleotide contains at least one phosphorothioate internucleoside linkage. In some embodiments, the oligonucleotide contains a mix of phosphorothioate internucleoside linkages and phosphodiester internucleoside linkages. In some embodiments, the oligonucleotide contains only phosphorothioate internucleoside linkages linking each pair of 2'-deoxyribonucleosides, and a mix of phosphorothioate internucleoside linkages and phosphodiester internucleoside linkages linking the remaining nucleosides.

[0124] In some embodiments, the oligonucleotide comprises a 5'-XYZ-3' configuration of LLEE-(D)8-EELL, where "E" is a 2'-MOE modified ribonucleoside; "L" is LNA; "D" is a 2'-deoxyribonucleoside; and "10" or "8" is the number of 2'-deoxyribonucleosides in Y, and where the oligonucleotide comprises phosphorothioate internucleoside linkages, phosphodiester internucleoside linkages, or a combination thereof.

[0125] In some embodiments, each cytidine (e.g., a 2'-modified cytidine) in oligonucleotide X and / or Z is optionally and independently a 5-methyl-cytidine and / or each uridine (e.g., a 2'-modified uridine) in oligonucleotide X and / or Z is optionally and independently a 5-methyl-uridine.

[0126] In some embodiments, the oligonucleotides described herein (eg, the DMPK-targeting oligonucleotides described herein) comprise a 5'-XYZ-3' configuration and comprise the nucleobase sequence CAGCGCCCACCAGUCA (SEQ ID NO: 21). In some embodiments, oligonucleotides described herein (e.g., DMPK-targeted oligonucleotides described herein) comprise a structure represented by +C*+A*oG*oC*dG*dC*dC*dC*dA*dC*dC*dA*oG*oU*+C*+A (SEQ ID NO: 21), where +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents a 5-methyl-2'-MOE-cytidine, +C represents a 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents a 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage.

[0127] In some embodiments, the oligonucleotides described herein (including, for example, the DMPK-targeting oligonucleotides described herein) have the formula (Ie): [ka] The structure includes:

[0128] In some embodiments, the oligonucleotides described herein (e.g., the DMPK-targeting oligonucleotides described herein) may be in the form of a salt, e.g., as a sodium salt, potassium salt, or magnesium salt.

[0129] In some embodiments, the 5' or 3' nucleoside (e.g., the terminal nucleoside) of the oligonucleotide is conjugated to an amine group, optionally via a spacer. In some embodiments, the spacer comprises an aliphatic moiety. In some embodiments, the spacer comprises a polyethylene glycol moiety. In some embodiments, a phosphodiester linkage is present between the spacer and the 5' or 3' nucleoside of the oligonucleotide. In some embodiments, the 5' or 3' nucleoside (e.g., the terminal nucleoside) of the oligonucleotide described herein is covalently linked to a spacer, said spacer being selected from the group consisting of substituted or unsubstituted aliphatic, substituted or unsubstituted heteroaliphatic, substituted or unsubstituted carbocyclylene, substituted or unsubstituted heterocyclylene, substituted or unsubstituted arylene, substituted or unsubstituted heteroarylene, -O-, -N(R A )-, -S-, -C(=O)-, -C(=O)O-, -C(=O)NR A -, -NR A C(=O)-, -NR A C(=O)R A -, -C(=O)R A -, -NR A C(=O)O-, -NR A C(=O)N(R A )-, -OC(=O)-, -OC(=O)O-, -OC(=O)N(R A )-, -S(O)NR A -, -NR A S(O)2-, or a combination thereof; each R A is independently hydrogen or substituted or unsubstituted alkyl. In some embodiments, the spacer is a substituted or unsubstituted alkylene, a substituted or unsubstituted heterocyclylene, a substituted or unsubstituted heteroarylene, -O-, -N(R A )-, or -C(=O)N(R A )2, or a combination of these.

[0130] In some embodiments, the 5' or 3' nucleoside of the oligonucleotide has the formula -NH2-(CH2) n -, where n is an integer from 1 to 12. In some embodiments, n is 6, 7, 8, 9, 10, 11, or 12. In some embodiments, the phosphodiester linkage is conjugated to a compound of the formula NH2-(CH2) n - is present between the compound represented by the formula NH2-(CH2)6- and the 5' or 3' nucleoside of the oligonucleotide. In some embodiments, the compound represented by the formula NH2-(CH2)6- is conjugated to the oligonucleotide via a reaction between 6-amino-1-hexanol (NH2-(CH2)6-OH) and the 5' phosphate of the oligonucleotide.

[0131] In some embodiments, the oligonucleotide is conjugated to a targeting agent, e.g., a muscle targeting agent such as an anti-TfR1 antibody, e.g., via an amine group of a lysine of the targeting agent.

[0132] It should be understood that in some embodiments, methylation of the nucleobase uracil at the C5 position forms thymine. Thus, in some embodiments, a nucleotide or nucleoside having a C5 methylated uracil (or 5-methyl-uracil) may be equivalently identified as a thymine nucleotide or nucleoside.

[0133] In some embodiments, any one or more of the thymine bases (T) in any one of the oligonucleotides provided herein may independently and optionally be uracil bases (U), and / or any one or more of the U's in the oligonucleotides provided herein (e.g., the oligonucleotide as set forth in SEQ ID NO:21) may independently and optionally be T.

[0134] composition In some embodiments, the compositions described herein include a complex (i.e., a plurality of complexes), each of which includes an antibody (e.g., an anti-TFR1 antibody) covalently linked to one or more oligonucleotides (e.g., a DMPK-targeting oligonucleotide described herein), wherein the antibody includes a heavy chain including a heavy chain variable region (VH) and a heavy chain constant region, and a light chain including a light chain variable region (VL) and a light chain constant region. In some embodiments, the antibody of such a complex includes CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 as described in Table 2. The complex of the compositions described herein may include any structure provided herein, for example, a structure represented by Formula (I) (including, for example, Formula (Ia), Formula (Ib), Formula (Ic), or Formula (Id)) or Formula (A).

[0135] In some embodiments, the compositions described herein comprise a conjugate (i.e., a plurality of conjugates), wherein each conjugate has the formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 independently includes compounds that include an oligonucleotide (e.g., a DMPK-targeting oligonucleotide as described herein), and R 2 wherein R 2 In some embodiments, each R of the complex comprises an antibody (e.g., an anti-TfR1 antibody) that comprises a heavy chain comprising a heavy chain variable region (VH) and a heavy chain constant region, and a light chain comprising a light chain variable region (VL) and a light chain constant region. 1 are independently 2 is covalently linked to a different amino acid residue (e.g., lysine).

[0136] In some embodiments, the value of n1 of the conjugate in the composition is independently and optionally from 1 to at most 100 μg of an antibody (e.g., R 2In some embodiments, the value of n1 of each conjugate in the composition is independently and optionally selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, and 27. In some embodiments, the value of n1 of each conjugate in the composition is independently and optionally selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, and 26. In some embodiments, the value of n1 for each conjugate in the composition is independently and arbitrarily selected from integers ranging from 1 to 27, 1 to 26, 1 to 10, 1 to 5, or 1 to 3. In some embodiments, the average value of n1 for the conjugates of the composition is in the range of 1 to 2, 1 to 3, 1 to 5, 1 to 10, 1 to 26, or 1 to 27. In some embodiments, the compositions described herein include a conjugate having a value of n1 of 0. In some embodiments, the average value of n1 of the complexes of the composition is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2).

[0137] In some embodiments, the compositions described herein include (e.g., a trace amount of) an antibody that is not conjugated to an oligonucleotide and an antibody that is conjugated to one or more oligonucleotides. In some embodiments, the antibody that is not conjugated to an oligonucleotide has the formula (I): [R 1 ] n1 -R 2where n1 is zero. Accordingly, in some embodiments, the composition for administration to a subject in the methods described herein may be a compound having a structure represented by formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 independently comprises a group that comprises an oligonucleotide; R 2 includes an antibody, and n1 independently represents R 1 In some embodiments, the compound represented by formula (I): [R 1 ] n1 -R 2 The proportion of compounds containing a structure represented by the formula (I) is less than 10%, less than 5%, less than 1%, less than 0.5%, less than 0.1%, less than 0.05%, or less than 0.01%, compared to all compounds represented by that structure in a composition in which n1 is 1 or more. Thus, in some embodiments, the average value of n1 of the complexes in the compositions disclosed herein is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2).

[0138] formulation The conjugates provided herein are formulated in a manner suitable for pharmaceutical use. In some embodiments, the conjugates can be delivered to a subject using a formulation that minimizes degradation, facilitates delivery and / or (for example and) uptake, or provides another beneficial property to the conjugate in the formulation. Thus, in some embodiments, formulating the conjugate (for example, a conjugate comprising an oligonucleotide covalently linked to a Fab) with tris(hydroxymethyl)aminomethane (also known as tromethamine or THAM) and / or sucrose has been found to be particularly advantageous for pharmaceutical use, as described herein for example. Thus, in some embodiments, provided herein is a formulation (for example, an aqueous solution, a lyophilized form, or a frozen form) that comprises the conjugate together with tris(hydroxymethyl)aminomethane and / or sucrose. Such formulations can be suitably prepared so that a sufficient amount of the conjugate can enter the target muscle cell when administered either into the environment surrounding the target cell of the subject or into the whole body of the subject.

[0139] In some embodiments, provided herein is a formulation comprising a complex (i.e., a plurality of complexes) comprising an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' arrangement) covalently linked to an antibody. In some embodiments, provided herein is a formulation comprising a complex, each complex of which comprises an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' arrangement) covalently linked to an anti-TfR1 antibody, optionally wherein the antibody of such complex comprises CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 as described in Table 2, and further wherein the complex is formulated with tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the antibody is an anti-TfR1 antibody.

[0140] In some embodiments, the compound of formula (I): [R 1 ]n1 -R 2 wherein each R 1 independently includes compounds that include oligonucleotides (e.g., oligonucleotides that include a 5'-XYZ-3' configuration), and R 2 includes an antibody (e.g., an anti-TfR1 antibody), and n1 is R 1 is an integer representing the number of instances of

[0141] In some embodiments, the formulations described herein comprise a conjugate comprising an antibody comprising CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 as described in Table 2. In some embodiments, the antibody comprises a VH comprising the amino acid sequence of SEQ ID NO: 17, and a VL comprising the amino acid sequence of SEQ ID NO: 18. In some embodiments, the antibody is a Fab and comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO:20.

[0142] In some embodiments, the formulations described herein contain the complexes described herein at a concentration of between 1 mg and 50 mg of complex per mL of formulation. In some embodiments, the formulations described herein contain the complexes at a concentration of 10-50 mg / mL or 20-35 mg / mL (e.g., 15-45 mg / mL, 20-40 mg / mL, 25-35 mg / mL, 25.5-34.5 mg / mL, 26-34 mg / mL, 27-33 mg / mL, 28-32 mg / mL, 29-31 mg / mL, 29.5-30.5 mg / mL, 10-40 mg / mL, 15-35 mg / mL, 20-30 mg / mL, 21-29 mg / mL, 21.2-28.8 mg / mL, 22-28 mg / mL, 23-27 mg / mL, 24-26 mg / mL, or 24.5-25.5 mg / mL). In some embodiments, the formulations described herein contain the complex at a concentration of approximately 25 mg / mL (e.g., 25 mg / mL). In some embodiments, the formulations described herein contain the complex at a concentration of approximately 30 mg / mL (e.g., 30 mg / mL). In some embodiments, the concentration of the complex in the formulation may vary by up to 20% (e.g., up to + / - 20%, up to + / - 15%, up to + / - 10%, or up to + / - 5%) of the set value. For example, in some embodiments, the concentration of the complex in the formulation is up to + / - 15% of 30 mg / mL (e.g., 30 + / - 4.5 mg / mL). For example, in some embodiments, the concentration of the complex in the formulation is up to + / - 15% of 25 mg / mL (e.g., 25 + / - 3.8 mg / mL).

[0143] In some embodiments, any one or more of the complexes described herein are formulated in an aqueous solution with tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration ranging from 5 to 50 mM (e.g., 5 to 40 mM, 5 to 35 mM, 5 to 30 mM, 10 to 50 mM, 15 to 45 mM, 10 to 40 mM, 20 to 40 mM, 20 to 35 mM, 20 to 30 mM, 21 to 29 mM, 22 to 28 mM, 23 to 27 mM, 24 to 26 mM). In some embodiments, the tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration of approximately 25 mM (e.g., 25 mM). In some embodiments, sucrose is present in the aqueous solution at a concentration ranging from 5% to 15% weight per volume (w / v%), e.g., 7-13% w / v%, 8-15% w / v%, 9-15% w / v%, 9-11% w / v%, 9.5-11% w / v%, or, e.g., 9-10 w / v%, 10-11 w / v%, 10-12 w / v%, or 8-12 w / v%. In some embodiments, sucrose is present in the aqueous solution at a concentration ranging from 7-13 w / v%, 8-12 w / v%, 9-11 w / v%. In some embodiments, sucrose is present in the aqueous solution at a concentration of approximately 10 w / v% (e.g., 10 w / v%). In some embodiments, the aqueous solution has a pH in the range of 6.5 to 8.5, e.g., 6.5 to 6.5, 6.7 to 6.9, 6.9 to 7.1, 7.1 to 7.3, 7.2 to 7.8, 7.3 to 7.5, 7.4 to 7.5, 7.4 to 7.6, 7.5 to 7.6; e.g., 7.0 to 8.0, or, e.g., 7.0 to 7.3, 7.2 to 7.8, 7.3 to 7.5, 7.4 to 7.6, 7.5 to 7.6, 7.5 to 7.7, 7.7 to 7.9, 7.9 to 8.0, 8.0 to 8.2, 8.2 to 8.4, 8.3 to 8.4, 8.4 to 8.5, 8.5 to 8.6, or 7.3 to 7.7. In some embodiments, the aqueous solution has a pH in the range of 7.0-8.0 (e.g., 7.0-7.8, 7.1-7.8, 7.2-7.8, 7.3-7.7, 7.3-7.5, 7.3-7.6, 7.4-7.6, or 7.4-7.8).In some embodiments, the aqueous solution has a pH of approximately 7.5 (e.g., 7.5). In some embodiments, the aqueous solution has a pH in the range of 7.4 to 7.7. In some embodiments, the aqueous solution has a pH in the range of 7.4 to 7.6 (e.g., 7.5 or about 7.5).

[0144] In some embodiments, any one of the formulations described herein is in an aqueous solution, wherein tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration of approximately 25 mM (e.g., 25 mM), wherein sucrose is present in the aqueous solution at a concentration of approximately 10 w / v% (e.g., 10 w / v%), and wherein the aqueous solution is at a pH of approximately 7.5 (e.g., 7.5).

[0145] In some embodiments, any one of the formulations described herein is in an aqueous solution, wherein tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration of approximately 25 mM (e.g., 25 mM), wherein sucrose is present in the aqueous solution at a concentration of approximately 10 w / v% (e.g., 10 w / v%), wherein the complex is present in the aqueous solution at a concentration of approximately 20-35 mg / ml (e.g., 25 mg / ml or 30 mg / ml), and wherein the aqueous solution is at a pH of approximately 7.5 (e.g., 7.5).

[0146] In some embodiments, any one or more of the complexes described herein are formulated with tris(hydroxymethyl)aminomethane and sucrose in a lyophilized form (e.g., a lyophilized powder). In some embodiments, the lyophilized form (e.g., a lyophilized powder) is obtained by lyophilization of any one of the aqueous solutions described herein.

[0147] In some embodiments, the lyophilized form is a lyophilized cake. In some embodiments, the lyophilized cake comprises a plurality of conjugates provided herein, tris(hydroxymethyl)aminomethane, and sucrose. In some embodiments, the lyophilized cake comprises 1x10 6 conjugates per gram of cake. -2 mg to 1.5 mg (for example, 1x10 -2 mg~1.5mg, 4.02x10 -2 mg~1.21mg, 6x10 -2 mg~1mg, 8x10 -2 mg-1 mg, 0.1 mg-1 mg, 0.05 mg-0.2 mg, 0.05 mg-0.3 mg, 0.05 mg-0.4 mg, 0.1 mg-0.2 mg, 0.1 mg-0.3 mg, 0.1 mg-0.4 mg, 0.1 mg-0.5 mg, 0.2 mg-0.3 mg, 0.2 mg-0.4 mg, 0.2 mg-0.4 mg, 0.3 mg-0.4 mg, or 0.3 mg-0.5 mg) tris(hydroxymethyl)aminomethane. In some embodiments, the lyophilized cake contains 900 mg to 1100 mg (e.g., 900 mg to 1100 mg, 989 mg to 999 mg, 950 mg to 1000 mg, 980 mg to 1000 mg, 980 mg to 1000 mg, 980 mg to 990 mg, 990 mg to 1000 mg, 995 mg to 1000 mg, 992 mg to 998 mg, 994 mg to 999 mg) of sucrose per gram of cake. In some embodiments, the lyophilized cake contains 0.5 mg to 10 mg (e.g., 0.5 mg to 10 mg, 0.5 mg to 1.5 mg, 1 mg to 2 mg, 1.5 mg to 3 mg, 1 mg to 8 mg, 0.5 mg to 6 mg, 0.5 mg to 5 mg, 1.5 mg to 3.5 mg, 1.5 mg to 4 mg, 1.5 mg to 5 mg, 2 mg to 5 mg, 2.5 mg to 5 mg, 2 mg to 3 mg, 3 mg to 4 mg, 4 mg to 5 mg) of the complex per gram of cake.

[0148] In some embodiments, the lyophilized cake contains 0.303 mg tris(hydroxymethyl)aminomethane per gram of cake, 998.6 mg sucrose per gram of cake, and / or (for example and) 0.999 mg complex per gram of cake. In some embodiments, the lyophilized cake contains 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 997.7 mg sucrose per gram of cake, and / or (for example and) 1.995 mg complex per gram of cake. In some embodiments, the lyophilized cake contains 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 997.2 mg sucrose per gram of cake, and / or (for example and) 2.49 mg complex per gram of cake. In some embodiments, the lyophilized cake contains 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 996.7 mg sucrose per gram of cake, and / or (for example and) 2.99 mg complex per gram of cake. In some embodiments, the lyophilized cake contains 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 995.7 mg sucrose per gram of cake, and / or (for example and) 3.98 mg complex per gram of cake. In some embodiments, the lyophilized cake contains 0.301 mg tris(hydroxymethyl)aminomethane per gram of cake, 994.7 mg sucrose per gram of cake, and / or (for example and) 4.97 mg complex per gram of cake.

[0149] In some embodiments, reconstitution of the lyophilized cake (e.g., with water) results in a solution containing 5-tris(hydroxymethyl)aminomethane at a concentration of 5-50 mM, sucrose at a concentration of 5% to 15% w / v, and / or (e.g., and) the complex at a concentration of 10 mg / mL to 50 mg / mL. In some embodiments, reconstitution of 10.053 g of lyophilized cake with a solvent (e.g., water) to form 1 mL of reconstituted solution results in a solution containing tris(hydroxymethyl)aminomethane at a concentration of 5-50 mM (optionally 25 mM), sucrose at a concentration of 5% to 15% w / v (optionally 10% w / v), and / or (e.g., and) the complex at a concentration of 10 mg / mL to 50 mg / mL. In some embodiments, reconstitution of about 10 g of lyophilized cake with a solvent (e.g., water) to form 1 mL of reconstituted solution results in a solution containing tris(hydroxymethyl)aminomethane at a concentration of 5 to 50 mM (optionally 25 mM), sucrose at a concentration of 5% w / v to 15% w / v (optionally 10% w / v), and / or (by way of example and) complex at a concentration of 10 mg / mL to 50 mg / mL.

[0150] In some embodiments, any one or more of the complexes described herein are formulated with tris(hydroxymethyl)aminomethane and sucrose in a frozen form (e.g., frozen aqueous solid). In some embodiments, the frozen form (e.g., frozen aqueous solid) is obtained by freezing any one of the aqueous solutions described herein. The frozen form may be frozen to a temperature below -20°C (e.g., below -20°C, below -30°C, below -40°C, below -50°C, below -60°C, below -70°C, below -80°C, or lower).

[0151] In some embodiments, the value of n1 for each conjugate in the formulation can be independently and optionally selected from zero to a maximum of 1000 mg of the antibody (R 2In some embodiments, the value of n1 of each conjugate in the formulation is independently and optionally selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, and 27. In some embodiments, the value of n1 of each conjugate in the formulation is independently and optionally selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, and 26. In some embodiments, the value of n1 for each conjugate in the formulation is independently and optionally selected from integers ranging from 1 to 27, 1 to 26, 1 to 10, 1 to 5, or 1 to 3. In some embodiments, the average value of n1 for the conjugates in the formulation is in the range of 1 to 2, 1 to 3, 1 to 5, 1 to 10, 1 to 26, or 1 to 27. In some embodiments, the formulations described herein comprise a compound of formula (I): [R 1 ] n1 -R 2 wherein n1 is 0. In some embodiments, the average value of n1 of the complex of the formulation is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2).

[0152] In some embodiments, R in the conjugates herein (e.g., the conjugates of the formulations provided herein) 1 Each instance of R is co-conjugated to a different amino acid residue of the antibody. In some embodiments, each different amino acid comprises an ε-amino group (e.g., lysine, arginine). However, in some embodiments, R 1In some embodiments, each different amino acid to which R is covalently linked is a cysteine. 1 is directly covalently linked to an amino acid residue of the antibody. 1 is indirectly covalently linked to an amino acid of the antibody, e.g., covalently linked to a glycosylation site on the amino acid. 1 In some embodiments, the R is present in trace amounts, undetectable amounts, or is completely absent. 1 In accordance with another aspect of the present invention, there is provided a preparation in which a conjugate in which is covalently linked to amino acid residues in the CDR regions of an antibody is undetectable in the preparation using standard detection techniques.

[0153] In some embodiments, the formulations provided herein comprise a compound represented by formula (I): [R 1 ] n1 -R 2 In the present invention, a complex containing a structure represented by the formula 1 Each instance of independently represents the formula (Ic): [ka] In the formula R 3 includes oligonucleotides, e.g., oligonucleotides comprising the 5'-XYZ-3' configuration; and R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 includes an antibody that is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. 3 comprises an oligonucleotide comprising the nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO: 21). 3comprises an oligonucleotide comprising the structure represented by +C*+A*oG*oC*dG*dC*dC*dC*dA*dC*dC*dA*oG*oU*+C*+A (SEQ ID NO:21), where +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-MOE modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage. In some embodiments, in each conjugate, n1 is independently an integer (e.g., an integer ranging from 1 to 27, 1 to 26, 1 to 10, 1 to 5, or 1 to 3). In some embodiments, the formulations described herein further comprise a compound represented by formula (I): [R 1 ] n1 -R 2 In some embodiments, the average value of n1 of the complex of the composition is within the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, the formulation further comprises tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the formulation comprises tris(hydroxymethyl)aminomethane at a concentration of 25 mM and / or (for example) sucrose at a concentration of 10 w / v%, optionally wherein the formulation has a pH of 7.5. In some embodiments, the formulation comprises the complex at a concentration of 30 mg / mL.

[0154] In some embodiments, the formulations provided herein comprise a compound represented by formula (I): [R 1 ] n1 -R2 wherein R in the conjugate of the formulation provided herein is 1 Each example has formula (Ib): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, * represents a phosphorothioate internucleoside linkage, and wherein the oligonucleotide comprises a nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21), wherein n1 represents the R 1 is an integer (e.g., 1 or greater) representing the number of instances of, and each R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2 comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 In some embodiments, the formulations described herein further comprise an antibody that is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. 1 ] n1 -R 2In some embodiments, the average value of n1 of the complex of the composition is within the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, the formulation further comprises tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the formulation comprises tris(hydroxymethyl)aminomethane at a concentration of 25 mM and / or (for example) sucrose at a concentration of 10 w / v%, optionally wherein the formulation has a pH of 7.5. In some embodiments, the formulation comprises the complex at a concentration of 30 mg / mL.

[0155] In some embodiments, the formulations provided herein comprise a compound represented by formula (I): [R 1 ] n1 -R 2 In the formula, each R 1 is represented by the formula (Ic): [ka] In the formula R 1 is R 2 At attachment point A, R 2 includes antibodies comprising a sequence as set forth in Table 2. For example, in some embodiments, R 2comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2 In some embodiments, the formulations described herein further comprise an antibody that is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. 1 ] n1 -R 2In some embodiments, the average value of n1 of the complex of the composition is within the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, the formulation further comprises tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the formulation comprises tris(hydroxymethyl)aminomethane at a concentration of 25 mM and / or (for example) sucrose at a concentration of 10 w / v%, optionally wherein the formulation has a pH of 7.5. In some embodiments, the formulation comprises the complex at a concentration of 30 mg / mL.

[0156] In some embodiments, the formulations provided herein have formula (Id): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, * represents a phosphorothioate internucleoside linkage, and wherein the oligonucleotide comprises a nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21); 2includes an antibody comprising a sequence as set forth in Table 2; where n1 is an integer (e.g., 1 or more) representing the number of instances of the group enclosed by the brackets, where each instance of the group enclosed by the brackets is covalently linked to a different amino acid residue of the antibody, optionally where each different amino acid residue is a lysine. For example, in some embodiments, R 2 comprises an antibody comprising a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 2 In some embodiments, R comprises an antibody comprising a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (e.g., at least 95%) identical to SEQ ID NO: 20. 2 In some embodiments, R comprises an antibody comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20. 2comprises an antibody that is a Fab fragment, a full length IgG, a Fab' fragment, a F(ab')2 fragment, an scFv, or an Fv. In some embodiments, the formulations described herein further comprise a conjugate where n1 is 0. In some embodiments, the average value of n1 of the complex of the composition is in the range of 0.5 to 5 (e.g., 0.5 to 5, 1 to 5, 1 to 4, 1 to 3, 3 to 5, 0.5 to 4, 0.5 to 3, 0.5 to 2, 0.5 to 1.5, 0.5 to 1, 0.7 to 1.5, 1 to 1.6, 1 to 1.5, 1 to 1.4, 1 to 1.3, 1 to 1.2, 1.1 to 1.5, 0.8 to 2, 0.8 to 1.5, 0.8 to 1.3, 0.8 to 1.2, 0.8 to 1.1, 0.9 to 3, 0.9 to 2, 0.9 to 1.8, 0.9 to 1.6, 0.9 to 1.5, 0.9 to 1.4, 0.9 to 1.3, or 0.9 to 1.2). In some embodiments, the formulation further comprises tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the formulation comprises tris(hydroxymethyl)aminomethane at a concentration of 25 mM and / or (by way of example) sucrose at a concentration of 10% w / v, optionally wherein the formulation has a pH of 7.5. In some embodiments, the formulation comprises the complex at a concentration of 30 mg / mL.

[0157] In some embodiments, the conjugate provided in the formulation described herein has the formula (A): [ka] where y is 0-15 (e.g., 3) and z is 0-15 (e.g., 4). In some embodiments, the amide shown adjacent to an antibody (e.g., an anti-TfR1 antibody) in structure (A) results from reaction with an amine of the antibody, such as a lysine epsilon amine. In some embodiments, the conjugates described herein include an anti-TfR1 antibody (e.g., an anti-TfR1 Fab) covalently linked through a lysine of the antibody to the 5' end of an oligonucleotide (e.g., an oligonucleotide comprising a 5'-XYZ-3' configuration), in some embodiments, the antibody comprises a sequence as set forth in Table 2. For example, in some embodiments, the antibody comprises a heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, a heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, a heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; and / or a light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, a light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and a light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. In some embodiments, the antibody comprises a heavy chain variable region (VH) comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 17, and / or a light chain variable region (VL) comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 18. In some embodiments, the antibody comprises a VH comprising the amino acid sequence of SEQ ID NO: 17, and / or a VL comprising the amino acid sequence of SEQ ID NO: 18. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 19, and / or a light chain comprising an amino acid sequence at least 85% (eg, at least 95%) identical to SEQ ID NO: 20. In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19, and / or a light chain comprising the amino acid sequence of SEQ ID NO: 20.In some embodiments, the antibody is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, a scFv, or an Fv. In some embodiments, the antibody is a Fab fragment. In some embodiments, the formulation further comprises tris(hydroxymethyl)aminomethane and sucrose. In some embodiments, the formulation comprises tris(hydroxymethyl)aminomethane at a concentration of 25 mM and / or (by way of example and) sucrose at a concentration of 10 w / v%, optionally wherein the formulation has a pH of 7.5. In some embodiments, the formulation comprises the complex at a concentration of 30 mg / mL.

[0158] As described herein, in some embodiments, the formulations provided herein include sucrose. In some embodiments, the sucrose acts at least in part as a lyoprotectant. In some embodiments, the sucrose is from a plant, e.g., a grass, fruit, or vegetable (e.g., a root vegetable) source (e.g., beet (e.g., sugar beet, e.g., Saccharum spp.), sugar cane (e.g., Beta vulgaris), date palm, sugar maple, sweet sorghum, apple, orange, carrot, molasses, maple syrup, corn sweetener), or an animal product (e.g., honey). In some embodiments, the sucrose is from beet or sugar cane (e.g., beet sucrose, sugar cane bisucrose). In some embodiments, a lyoprotectant other than sucrose may also be used, e.g., trehalose, mannitol, lactose, polyethylene glycol, or polyvinylpyrrolidone. However, in some embodiments, a collapse temperature modifier (eg, dextran, ficoll, or gelatin) may be provided in the formulation.

[0159] In some embodiments, provided is a product (e.g., a lyophilized formulation described herein) produced by a process comprising lyophilizing an aqueous solution of a formulation (e.g., an aqueous form) described herein.

[0160] In some embodiments, a pharmaceutical composition is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, and subcutaneous. Typically, the route of administration is intravenous or subcutaneous.

[0161] Method of Use / Treatment A conjugate comprising an anti-TfR1 antibody (e.g., a Fab) covalently linked to a molecular payload (e.g., a DMPK-targeting oligonucleotide) as described herein is effective in treating a subject with myotonic dystrophy, e.g., DM1. In some embodiments, the conjugate comprises a molecular payload that is an oligonucleotide, e.g., an oligonucleotide that facilitates reduced expression or activity of DMPK (e.g., reduced levels of mutant or wild-type DMPK RNA).

[0162] In some embodiments, the subject may be a human subject, a non-human primate subject, a rodent subject, or any suitable mammalian subject.In some embodiments, the subject may have myotonic dystrophy.In some embodiments, the subject has a DMPK allele that may optionally contain disease-associated repeats, for example CTG trinucleotide repeat expansions. In some embodiments, the subject may have a DMPK allele with an expanded disease-associated repeat comprising about 2-10 repeat units, about 2-50 repeat units, about 2-100 repeat units, about 50-1,000 repeat units, about 50-500 repeat units, about 50-250 repeat units, about 50-100 repeat units, about 500-10,000 repeat units, about 500-5,000 repeat units, about 500-2,500 repeat units, about 500-1,000 repeat units, or about 1,000-10,000 repeat units. In some embodiments, the subject may have a myotonic dystrophy, such as DM1. In some embodiments, the subject suffers from symptoms of DM1, such as muscle atrophy, muscle loss, excessive daytime sleepiness, or retardation. In some embodiments, the subject does not suffer from symptoms of DM1. In some embodiments, the subject has congenital myotonic dystrophy. In some embodiments, the subject is ambulatory. In some embodiments, the subject is non-ambulatory.

[0163] One aspect of the disclosure includes a method involving administering to a subject a formulation comprising an effective amount of a conjugate(s) as described herein. In some embodiments, an effective amount of a pharmaceutical composition comprising a conjugate(s) comprising an antibody (e.g., a Fab) as described herein covalently linked to an oligonucleotide (e.g., a DMPK-targeting oligonucleotide) as described herein may be administered to a subject in need of treatment. In some embodiments, a pharmaceutical composition comprising a conjugate(s) as described herein may be administered by a suitable route, which may include intravenous administration, e.g., as a bolus or by continuous infusion over a period of time. In some embodiments, administration may be performed by intravenous, intramuscular, intraperitoneal, intracerebrospinal, subcutaneous, intraarticular, intrasynovial, or intrathecal routes. In some embodiments, the pharmaceutical composition may be in a solid, aqueous, or liquid form. In some embodiments, the aqueous or liquid form may be sprayed or lyophilized. In some embodiments, the lyophilized form may be reconstituted with an aqueous or liquid solution.

[0164] In some embodiments, provided are methods and / or uses for treating a subject with a DMPK allele, which may optionally contain a disease-associated repeat, comprising administering to the subject a formulation comprising an effective amount of a complex(es) described herein. In some embodiments, provided are methods and / or uses for reducing the expression or activity of DMPK (e.g., reducing the level of mutant or wild-type DMPK RNA, or the activity of a DMPK gene product) in a cell (e.g., a muscle cell), comprising contacting the cell with a formulation comprising an effective amount of a complex(es) described herein. In some embodiments, the method comprises administering a lyophilized form (e.g., a lyophilized powder) from a formulation described herein, reconstituting the lyophilized form of the formulation in an aqueous solution, and administering the aqueous solution of the formulation to a subject in need thereof. For example, in some embodiments, a lyophilized form of the formulation is shipped and / or stored in lyophilized form, reconstituted at a location for administration of an aqueous solution of the formulation (e.g., at a health care provider's location), and administered in the reconstituted form (e.g., as an aqueous solution) by injection or intravenously, e.g., by infusion.

[0165] In some embodiments, the pharmaceutical composition is administered via site-specific or local delivery techniques, examples of which include an implanted depot source of the complex, a local delivery catheter, a site-specific carrier, direct injection, or direct application.

[0166] In some embodiments, a pharmaceutical composition comprising a conjugate comprising an anti-TfR1 antibody (e.g., fab) covalently linked to a molecular payload (e.g., DMPK-targeting oligonucleotide) is administered at an effective concentration that confers a therapeutic effect to the subject. The effective amount will vary depending on the severity of the disease, the specific characteristics of the subject being treated, such as age, physical condition, health, or weight, the duration of the treatment, the nature of any concomitant therapy, the route of administration, and related factors, as will be recognized by those skilled in the art. These related factors are known to those skilled in the art and can be addressed with a little routine experimentation. In some embodiments, the effective concentration is the maximum dose considered safe for the patient. In some embodiments, the effective concentration will be the lowest concentration feasible that provides maximum efficacy.

[0167] Empirical considerations, for example the half-life of the complex(es) in the subject, will generally contribute to the determination of the concentration of pharmaceutical composition used for treatment. The frequency of administration may be empirically determined and adjusted to maximize the efficacy of treatment. The efficacy of treatment may be assessed using any suitable method. In some embodiments, the efficacy of treatment may be assessed by evaluation or observation of symptoms associated with myotonic dystrophy (for example muscle atrophy or muscle weakness), through subject self-reported outcomes, for example mobility, self-care, ability to perform daily activities, pain / discomfort, and anxiety / depression measures, or by quality of life indicators, for example life span. In some embodiments, a pharmaceutical composition comprising a conjugate comprising a muscle targeting agent covalently linked to a molecular payload described herein is administered to a subject at an effective concentration sufficient to modulate the activity or expression of a target gene by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or at least 95% compared to a control (e.g., a baseline level of gene expression prior to treatment).

[0168] example Example 1. In vivo tissue distribution of conjugates containing anti-TfR1 Fab conjugated to DMPK-targeted oligonucleotides in a DM1 mouse model A conjugate comprising an anti-TfR1 Fab conjugated to a DMPK-targeting oligonucleotide (ASO) was tested in a mouse model expressing both human TfR1 and a human DMPK mutant carrying an expanded CUG repeat. The anti-TfR1 Fab used has the VH / VL sequence provided in Table 2. The Fab was covalently linked (through lysine conjugation) via a linker comprising a valine-citrulline sequence to a DMPK-targeting oligonucleotide comprising the nucleobase sequence of SEQ ID NO:21. The conjugate has the formula (Id): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, * represents a phosphorothioate internucleoside linkage, and wherein the oligonucleotide comprises a nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21), and wherein R 2 is an anti-TfR1 Fab provided in Table 2, and where in each conjugate, n1 is independently an integer from 1 to 3.

[0169] The conjugate was intravenously administered to mice on days 0 and 7 at a dose equivalent to 9.7 mg / kg ASO each time. Tissue exposure of ASO was tested by hybridization ELISA (Burki et al., Nucleic Acid Ther. 2015 Oct;25(5):275-84, incorporated herein by reference), and the levels of ASO in tissues were graphed. Figure 1A, Figure 1B, Figure 1C, and Figure 1D show the amount of ASO in the heart, diaphragm, gastrocnemius, or tibialis anterior muscle, respectively, two weeks after the first injection. These results demonstrate that conjugates containing anti-TfR1 antibodies (for example, anti-TfR1 Fab with the VH and VL sequences provided in Table 2) can deliver oligonucleotides (for example, DMPK-targeted ASO) to various muscle tissues after intravenous administration.

[0170] Example 2. Sustained knockdown of toxic human DMPK in hTfR1 / DMSXL homozygous mice 4 weeks after repeated dosing of anti-TfR1 Fab-ASO conjugate Conjugates containing anti-TfR1 Fab covalently linked to DMPK-targeting oligonucleotides (ASOs) as described in Example 1 (labeled as "anti-TfR1 Fab-ASO conjugates" in this example) were tested in a mouse model (hTfR1 / DMSXL mice) expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CUG repeat. Mice were administered either vehicle control (PBS) or a 10 mg / kg ASO-equivalent dose of anti-TfR1 Fab-ASO conjugate on days 0 and 7. Mice were sacrificed on day 28 (4 weeks after administration of the first dose of anti-TfR1 Fab-ASO conjugate) and tissues were collected. RNA was extracted and selected tissue samples were fixed, paraffin-embedded, sectioned, and then subjected to in situ hybridization. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) of RNA samples was performed to measure human DMPK and mouse Ppib (peptidyl prolyl isomerase) as an internal control. DMPK expression is shown in Figures 2A-2D as geometric mean + / - standard deviation (n=6-9). Significance was assessed by Student's t-test (****P<0.0001).

[0171] Figure 2A shows that anti-TfR1 Fab-ASO conjugates knocked down DMPK expression in the heart by 49% compared to PBS-treated mice. Figure 2B shows that anti-TfR1 Fab-ASO conjugates knocked down DMPK expression in the diaphragm by 40% compared to PBS-treated mice. Figure 2C shows that anti-TfR1 Fab-ASO conjugates knocked down DMPK expression in the tibialis anterior muscle by 49% compared to PBS-treated mice. Figure 2D shows that anti-TfR1 Fab-ASO conjugates knocked down DMPK expression in the gastrocnemius muscle by 44% compared to PBS-treated mice.

[0172] Figures 3A and 3B show that anti-TfR1 Fab-ASO conjugates reduced DMPK-ForS in the nuclei of myofibers. Figure 3A shows reduced DMPK-ForS by in situ hybridization, and Figure 3B shows quantification of DMPK-ForS in fluorescent microscopy images, demonstrating that the conjugates reduced ForS area by 49%. Data are presented as mean + / - standard deviation (n=7). Significance was assessed by t-test (*P<0.05).

[0173] These results demonstrate that administration of the anti-TfR1 Fab-ASO conjugate leads to robust and sustained knockdown of human toxic DMPK in cardiac and skeletal muscle.

[0174] Example 3. Correction of splicing defects in hTfR1 / DMSXL homozygous mice by anti-TfR1 Fab-ASO conjugates A conjugate as described in Example 2 (labeled in this example as "anti-TfR1 Fab-ASO conjugate") containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) was tested in a mouse model expressing both human TfR1 and two copies of a mutant human DMPK transgene carrying an expanded CUG repeat ("hTfR1 / DMSXL"). These mice are known to display splicing defects consistent with those observed in patients suffering from DM1 (Huguet, et al. (2012) PLOS Genetics 8(11):e1003043). Mice were administered either a vehicle control ("hTfR1 / DMSXL-PBS") or a 10 mg / kg ASO-equivalent dose of the anti-TfR1 Fab-ASO conjugate ("hTfR1 / DMSXL-conjugate") on days 0 and 7. Mice expressing only human TfR1 but not mutant human DMPK transgene (hTfR1 mice) and mice treated with PBS ("hTfR1-PBS") were used as additional controls to define the extent of the splicing phenotype in hTfR1 / DMSXL mice and to assess the magnitude of the effect of the conjugate on splicing. Mice were sacrificed on day 28 (4 weeks after administration of the first dose of anti-TfR1 Fab-ASO conjugate), tissues were collected, and RNA was extracted. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to measure exon inclusion in a set of RNAs known to be misspliced ​​during DM1 progression in humans and mice (Nakamori, et al. (2013) Ann. Neurol. 74(6):862-872; Huguet, et al. (2012) PLOS Genetics 8(11):e1003043).Exon inclusion was calculated as the normalized percent spliced ​​in (PSI) for each splicing RNA marker, and composite splicing indices were calculated using the normalized PSI values ​​from splicing markers in the heart (Figure 4), diaphragm (Figure 5), tibialis anterior (Figure 6), and gastrocnemius (Figure 7). Composite splicing indices were calculated as previously described (Tanner MK, et al. (2021) Nucleic Acids Res. 49: 2240-2254) and are shown as the mean + / - standard deviation.

[0175] 4 shows that anti-TfR1 Fab-ASO conjugates corrected splicing in cardiac tissue from hTfR1 / DMSXL mice, as demonstrated by composite splicing index data. The normalized PSI values ​​used to generate composite splicing index data showed correction of Mbnl2 exon 6 (E6) and Nfix E7 splicing, but not Ldb3 E11 splicing, in cardiac tissue from hTfR1 / DMSXL mice upon treatment with anti-TfR1 Fab-ASO conjugates. The composite splicing index data shown in Figure 4 was based on splicing data for Ldb3 E11, Mbnl2 E6, and Nfix E7; Bin1 E11, Dtna E12, Insr E11, and Mbnl2 E5 were not included because their normalized PSI values ​​in cardiac tissue were not altered in hTfR1 / DMSXL mice compared to hTfR1 mice under the experimental conditions tested.

[0176] 5 shows that anti-TfR1 Fab-ASO conjugates corrected splicing in diaphragm tissue of hTfR1 / DMSXL mice, as demonstrated by composite splicing index data. The normalized PSI values ​​used to generate composite splicing index data showed correction of splicing of Bin1 E11, Insr E11, Ldb3 E11, and Nfix E7 in diaphragm tissue of hTfR1 / DMSXL mice by treatment with anti-TfR1 Fab-ASO conjugates. The composite splicing index data shown in Figure 7 was based on splicing data for Bin1 E11, Insr E11, Ldb3 E11, and Nfix E7; Dtna E12, Mbnl2 E5, Mbnl2 E6, and Ttn E313 were not included because their normalized PSI values ​​in diaphragm tissue were unchanged in hTfR1 / DMSXL mice compared to hTfR1 mice under the experimental conditions tested.

[0177] 6 shows that anti-TfR1 Fab-ASO conjugates corrected splicing in tibialis anterior muscle tissue of hTfR1 / DMSXL mice, as demonstrated by composite splicing index data. The normalized PSI values ​​used to generate composite splicing index data showed correction of Bin1 E11, Ldb3 E11, and Nfix E7 splicing, but not Mbnl2 E6 splicing, in tibialis anterior muscle tissue of hTfR1 / DMSXL mice by treatment with anti-TfR1 Fab-ASO conjugates. The composite splicing index data shown in Figure 6 was based on splicing data for Bin1 E11, Ldb3 E11, Mbnl2 E6, and Nfix E7; Dtna E12, Insr E11, Mbnl2 E5, and Ttn E313 were not included because their normalized PSI values ​​in tibialis anterior muscle tissue were not altered in hTfR1 / DMSXL mice compared to hTfR1 mice under the experimental conditions tested.

[0178] 7 shows that anti-TfR1 Fab-ASO conjugates did not correct splicing in gastrocnemius tissue from hTfR1 / DMSXL mice, as demonstrated by composite splicing index data. The normalized PSI values ​​used to generate composite splicing index data showed correction of splicing of Mbnl2 E6, Nfix E7, and Ttn E313 in gastrocnemius tissue from hTfR1 / DMSXL mice by treatment with anti-TfR1 Fab-ASO conjugates. The composite splicing index data shown in Figure 7 was based on splicing data for Mbnl2 E6, Nfix E7, and Ttn E313; Bin1 E11, Dtna E12, Insr E11, Ldb3 E11, and Mbnl2 E5 were not included because their normalized PSI values ​​in gastrocnemius muscle tissue were not altered in hTfR1 / DMSXL mice compared to hTfR1 mice under the experimental conditions tested.

[0179] These results demonstrate that administration of the anti-TfR1 Fab-ASO conjugate facilitates the correction of the DM1 splicing defect in cardiac and skeletal muscles.

[0180] Example 4. DMPK knockdown in non-human primates and DM1 patient myotubes A conjugate as described in Example 1 (labeled in this example as "anti-TfR1 Fab-ASO conjugate") containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO) was tested in human DM1 patient myotubes (32F cells) and in non-human primate (NHP) myotubes. The DM1 patient myotubes used express both mutant DMPK mRNA containing 380 CUG repeats and wild-type DMPK mRNA. The NHP myotubes used express only wild-type DMPK.

[0181] DM1 patient cells or NHP cells were seeded in 96-well plates in growth medium at a density of 50,000 cells per well and allowed to recover overnight. The next day, growth medium was replaced with low serum differentiation medium and cells were treated with conjugates at concentrations equivalent to 125 nM, 250 nM, or 500 nM ASO. Cells were incubated for 10 days and then cDNA was synthesized using the Cells-to-Ct kit with crude cell lysate as the source of total RNA.

[0182] cDNA was used to assess total DMPK knockdown using Taqman PCR. Data were normalized to PPIB expression and -ΔΔCt The method was used to determine DMPK knockdown relative to PBS-treated controls ("vehicle"). Data shown in Figure 8 are presented as mean DMPK expression + standard deviation (n=4 replicates per condition) relative to species-matched vehicle controls.

[0183] The results show that the anti-TfR1 Fab-ASO conjugate, when administered at physiologically relevant concentrations (FIG. 8), achieved knockdown of DMPK expression in both normal NHP myotubes and DM1 patient myotubes, with greater knockdown of DMPK expression in DM1 patient cells (expressing both DMPK mRNA containing 380 CUG repeats and wild-type DMPK mRNA) compared to NHP cells (expressing only wild-type DMPK mRNA). At an ASO equivalent concentration of 125 nM, the conjugate achieved approximately 40% DMPK knockdown compared to vehicle-only controls in NHP myotubes and approximately 65% ​​DMPK knockdown in DM1 patient myotubes. At an ASO equivalent concentration of 250 nM, the conjugate achieved approximately 45% DMPK knockdown compared to vehicle-only controls in NHP myotubes and approximately 80% DMPK knockdown in DM1 patient myotubes. The conjugate achieved approximately 60% DMPK knockdown compared to vehicle only controls in NHP myotubes and approximately 90% DMPK knockdown in DM1 patient myotubes at an ASO equivalent concentration of 500 nM.

[0184] These results indicate that a conjugate containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide can achieve greater knockdown of DMPK in human myotubes expressing both wild-type and mutant DMPK mRNA (in which the CUG repeat is expanded) compared to cynomolgus monkey myotubes expressing wild-type DMPK.

[0185] Example 5. Comparison of heat stability of different exemplary formulations Formulations were prepared containing the conjugate as described in Example 1, containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO). The starting material (SM) of the anti-TfR1 Fab-oligonucleotide conjugate was at a concentration of 33 mg / mL in a buffer containing 100 mM sodium phosphate and 100 mM sodium chloride at pH 7.4. For stability studies, the anti-TfR1 Fab-oligonucleotide conjugate was formulated at a concentration of approximately 40 mg / mL in various buffers. The buffer conditions in each of the four formulations were as follows: Formulation Buffer 1: 25 mM succinate, 10% (w / v) sucrose, pH 5.2. Formulation buffer 2: 25 mM succinate, 10% (w / v) sucrose, pH 6.0. Formulation Buffer 3: 25 mM tris(hydroxymethyl)aminomethane, 10% (w / v) sucrose, pH 7.5. Formulation Buffer 4: 100 mM Sodium Phosphate, 100 mM Sodium Chloride, pH 7.4.

[0186] The bulk formulation (formulation with the conjugate described herein) was buffer exchanged into formulation buffers 1-4 as provided above using Vivaspin® 6 centrifugal membrane filters (30 kDa molecular weight cutoff). The anti-TfR1 Fab-oligonucleotide conjugates were concentrated to approximately 40 mg / mL after buffer exchange. Approximately 150 μL aliquots from each of the concentrated samples were used to study the thermal stability of the conjugate in each formulation. Samples were incubated in glass vials at −20° C., 2-8° C., 25° C., or 40° C. for 1 week ("T1"), 2 weeks ("T2"), 4 weeks ("T4"), or 8 weeks ("T8"). No changes in color, appearance, or pH were observed in any of the test samples at any of the temperatures or time points tested.

[0187] The thermal stability of each formulation was first tested by measuring the concentration of the conjugate in various samples at T0 (week 0), T4, and T8 and at the specified temperatures. The concentration was measured using a standard BCA (bicinchoninic acid) assay. The BCA assay was performed using standard procedures. 1) Working Reagent (WR) was prepared by thoroughly mixing 20 mL of BCA Reagent A with 200 μL BCA Reagent B. 2) Standard curves and dilution curves for test samples were prepared. Test samples were first diluted 1:4, then 20 μL of each test sample or standard was thoroughly mixed with 200 μL of WR and incubated at 60° C. for 10 minutes. The resulting solution was added to the first row of a 96-well plate and serially diluted. A 6-point standard curve was prepared. 3) The absorbance at 480 nm was measured on a plate reader. 4) The protein concentration of each test sample was determined using the standard curve. 3) The absorbance at 480 nm was measured on a plate reader.

[0188] The concentration measurements of the thermal stability study are shown in Table 3 below. Table 3. [Table 3]

[0189] The results in Table 3 show that the concentrations of Fab-oligonucleotide conjugates in formulations 2, 3, and 4 were consistent at T0, T4, and T8. The concentrations of Fab-oligonucleotide conjugates at T4 and T8 in formulation 1 showed reduced concentrations at lower storage temperatures compared to the T0 measurements.

[0190] The thermal stability of the Fab-oligonucleotide conjugates was further studied by measuring aggregate formation at a concentration of 40 mg / mL. After dilution to 1 mg / mL, samples of T0, T1, T2, T4, and T8 incubated at the various temperatures specified were analyzed by size exclusion chromatography (SEC-UV) using a Waters ACQUITY UPLC Protein BEH SEC column (125A, 1.7 μm, 4.6×150 mm) with a mobile phase of 0.2 M potassium phosphate (pH 6.8), 0.2 M potassium chloride isocratic elution, 15% isopropyl alcohol. The column temperature was 30° C. and a 10 μg injection was used. The flow rate was 0.35 mL / min and the analysis was performed at a wavelength of 280 nm.

[0191] The percentage of high molecular weight (HMW) species, Fab-oligonucleotide conjugate monomer, and low molecular weight (LMW) species were determined for each sample in a SEC thermal stability assay, and the results are shown in Table 4 below. Values ​​for the starting material (SM) are shown in the top row for comparison. Table 4. [Table 4-1] [Table 4-2]

[0192] The results did not show any significant change in percent monomer with increasing temperature or time, and a slight increase in the LMW molecular species with increasing temperature was observed over time.

[0193] Overall, SEC analysis indicated that formulations 1, 2, and 3 offered slightly improved stability in terms of % monomer at the 8 week time point compared to formulation 4. Formulation 3 showed the best stability in terms of change in % LMW species.

[0194] The thermal stability of the Fab-oligonucleotide conjugates at 40 mg / mL was further studied by a non-reducing protein expression assay using capillary electrophoresis (CE-SDS). For analysis by this method, samples were diluted to 1 mg / mL and then mixed 1:1 with 10 mM N-ethylmaleimide in PBS. 5 μL of each of these samples was then added to 7 μL of sample buffer (Protein Express Reagent Kit, Perkin Elmer CLS960008) in a 96-well plate. Samples were incubated at 70° C. for 10 min and then chilled on ice for 5 min. 32 μL of water was added to each sample well and the plate was centrifuged at 1200 g for 2 min prior to analysis. Samples were analyzed on a HT Protein Express LabChip (Perkin Elmer 760499) using a LabChip GXII Touch HT (Perkin Elmer) using the assay HT High Sensitivity Antibody Analysis 200.

[0195] The results of the non-reduced CE-SDS analysis are shown below in Table 5. For comparison, the starting material exhibited a size of 62.4 kDa with a purity of 100.0%. Table 5. [Table 5]

[0196] The results showed that the percent purity of the conjugate in formulation 1 gradually decreased from T1 to T8 at 25° C. and 40° C., and the formation of new species was observed, suggesting that the conjugate in formulation 1 is prone to instability at high temperatures. The results also showed that the conjugate had good percent purity in formulations 2, 3, and 4 at all temperatures and time points tested. The high percent purity of the conjugate in formulation 3 at high temperatures suggests improved stability of formulation 3 compared to the other formulations.

[0197] The thermal stability of the Fab-oligonucleotide conjugates at 40 mg / mL was further studied by a reduced protein expression assay using capillary electrophoresis (CE-SDS). For analysis by this method, samples were diluted to 0.5 mg / mL in PBS. Samples were then mixed with denaturing solution: 24.5 μL of 14.2 M β-mercaptoethanol (BME) was added to 700 μL of Protein Express Sample Buffer (Protein Express Reagent Kit, Perkin Elmer CLS960008), and then 5 μL of each of the diluted samples was added to 7 μL of sample denaturing solution in a 96-well plate. Samples were incubated at 70° C. for 10 min and then chilled on ice for 5 min. 32 μL of water was added to each sample well and the plate was centrifuged at 1200 g for 2 min prior to analysis. Samples were analyzed on a HT Protein Express LabChip (Perkin Elmer 760499) using a LabChip GXII Touch HT (Perkin Elmer) using the assay HT High Sensitivity Antibody Analysis 200.

[0198] The results of the reduced CE-SDS analysis are shown below in Table 6. Values ​​for the starting material (SM) are shown in the top row for comparison. Table 6. [Table 6-1] [Table 6-2] [Table 6-3]

[0199] Formulation 1 had two additional peaks, including one additional HC peak observed in all samples at T2, T4, and T8, indicating that the conjugate in this formulation was unstable. There were no significant differences in the overall stability of the conjugate in formulations 2, 3, and 4 observed by reduced CE-SDS.

[0200] Overall, the results of the various thermal stability studies presented herein suggest that formulation 3 provided the best stability for the Fab-oligonucleotide conjugates.

[0201] Example 6. Comparison of freeze / thaw stability of different exemplary formulations Formulations were prepared containing the conjugate as described in Example 1, containing an anti-TfR1 Fab covalently linked to a DMPK-targeting oligonucleotide (ASO). The starting material (SM) of the anti-TfR1 Fab-oligonucleotide conjugate was at a concentration of 33 mg / mL in a buffer containing 100 mM sodium phosphate and 100 mM sodium chloride at pH 7.4. For freeze / thaw stability studies, the anti-TfR1 Fab-oligonucleotide conjugate was formulated at a concentration of approximately 40 mg / mL in various buffers. The buffer conditions in each of the four formulations were as follows: Formulation Buffer 1: 25 mM succinate, 10% (w / v) sucrose, pH 5.2. Formulation buffer 2: 25 mM succinate, 10% (w / v) sucrose, pH 6.0. Formulation Buffer 3: 25 mM tris(hydroxymethyl)aminomethane, 10% (w / v) sucrose, pH 7.5. Formulation Buffer 4: 100 mM Sodium Phosphate, 100 mM Sodium Chloride, pH 7.4.

[0202] To test the freeze / thaw stability of the conjugate in each formulation, aliquots were prepared and subjected to 0, 1, 3, or 5 freeze / thaw cycles, after which time points were assessed for visual appearance, protein concentration, and aggregate / fragment formation.

[0203] For each of Formulations 1, 2, 3, and 4, visual appearance assessment showed that the solutions were clear, colorless, and free of visible aggregates after 0, 1, 3, and 5 freeze / thaw cycles.

[0204] BCA analysis of each formulation, performed as described in Example 5, indicated that there was no significant change in the concentration of the complex over five freeze / thaw ("F / T") cycles. The results are shown in Table 7 below. Values ​​for the starting material (SM) are shown in the top row for comparison. Table 7 [Table 7]

[0205] Size exclusion chromatography (SEC) analysis of each formulation, performed as described in Example 5, suggested no significant differences between the four formulations with respect to low molecular weight (LMW), monomeric, and high molecular weight (HMW) species over five freeze / thaw ("F / T") cycles. The results are shown in Table 8 below. Values ​​for the starting material (SM) are shown in the top row for comparison. Table 8 [Table 8]

[0206] Example 7. Evaluation of the stability of lyophilized formulations of conjugates Conjugates containing anti-TfR1 Fab covalently linked to DMPK-targeting oligonucleotides (ASOs) as described in Example 1 (labeled in this example as "anti-TfR1 Fab-ASO conjugates") were prepared, lyophilized in tris(hydroxymethyl)aminomethane (25 mM), sucrose (10% (w / v)) at a pH of 7.5, and tested for stability. Prior to reconstitution, all samples at all time points and temperatures were visually identified as white cakes and were uniform in shape. In addition, samples reconstituted in less than 1 minute and became clear and colorless with fewer than 10 particles. All samples were deemed stable for at least 8 weeks and up to 40°C by visual analysis before and after reconstitution.

[0207] X-ray diffraction analysis was carried out to evaluate the crystalline structure of the lyophilized composition.Approximately 20mg of lyophilized anti-TfR1 Fab-ASO conjugate was placed on a plastic sample holder and flattened with a glass slide to ensure a flat surface.This analysis was carried out using a Proto AXRD® benchtop power X-ray diffractometer with a fine focus Cu-anode metal / ceramic structure 1500 watt X-ray tube.

[0208] The diffraction pattern was measured (Figure 9) and a broad "halo" (circled in Figure 9) was identified, indicating that the material was amorphous at time 0 (T0).

[0209] To test the absorbance of the samples, lyophilized anti-TfR1 Fab-ASO conjugates were first reconstituted with water and then diluted to a concentration of 0.2 mg / mL using formulation buffer (25 mM tris(hydroxymethyl)aminomethane, 10% (w / v) sucrose, pH 7.5). Absorbance was measured at 260 nm and 280 nm using a DeNovix DS11+ spectrophotometer. Readings were performed in triplicate and averaged. These results were used to calculate concentrations (Fab (mg / mL and μM) and oligonucleotide (μM)), percent recovery, and drug-antibody ratios (DAR) at multiple time points. For all protein concentration, percent recovery, and DAR studies, bovine serum albumin (BSA) was used as a positive control, PBS was used as a blank and negative control for BSA, and formulation buffer was used as a blank and negative control for conjugate samples.

[0210] The mean concentrations of replicates for Fab at 2-8°C (Figures 10A and 10B) showed no significant change from time 0 (T0) when compared to 4 weeks (T4w) or 8 weeks (T8w). In addition, there was no significant change when comparing the 4 and 8 week time points. The mean concentrations of replicates for oligonucleotide at 2-8°C (Figure 10C) showed no significant differences when comparing T0, T4w, and T8w. The mean percent recovery at 2-8°C (Figure 11A) was close to 100% for all samples at all time points. The mean DAR at 2-8°C (Figure 11B) was close to 1.0 at all time points.

[0211] The mean concentrations of replicates for Fab at 25° C. (FIGS. 12A and 12B) showed no significant change from time 0 (T0) when compared to 2 weeks (T2w), 4 weeks (T4w), or 8 weeks (T8w), and there were no significant changes when comparing T2w, T4w, and T8w to each other. The mean concentrations of replicates for oligonucleotides at 25° C. (FIG. 12C) showed no significant change over time. The mean percent recovery at 25° C. (FIG. 13A) was close to 100% at all time points. The mean DAR at 25° C. (FIG. 13B) was close to 1.0 at all time points.

[0212] The mean replicate concentrations for Fab at 40° C. (FIG. 14 and FIG. 14B) showed no significant change from TO when compared to T2w, T4w, or T8w. The mean replicate concentrations for oligonucleotide at 40° C. (FIG. 10C) showed no significant difference at any of the time points. The mean percent recovery at 40° C. (FIG. 15A) was close to 100% with all samples at all time points. The mean DAR at 40° C. (FIG. 15B) was close to 1.0 at all time points.

[0213] The anti-TfR1 Fab-ASO conjugates were then analyzed using size exclusion chromatography (SEC). All samples were diluted to a concentration of 1.0 mg / mL using formulation buffer (25 mM tris(hydroxymethyl)aminomethane, 10% (w / v) sucrose, pH 7.5) prior to analysis. For the mobile phase of the chromatography conditions, an isocratic eluent containing potassium phosphate (200 mM), potassium chloride (200 mM), 5% (v / v) isopropanol (IPA) at a pH of 6.8 was used at a flow rate of 0.250 mL / min using a run time of 20 minutes. The wavelength used for the measurement was 280 nm. The high performance liquid chromatography system used was a Dionex Ultimate 3000 UPLC system with a Waters BEH SEC column (200A, 300x4.6mm, 1.7μm, P / N 186005226).

[0214] The sum of the relative areas of the first and second (main) peaks at 2-8° C. (FIG. 16A) shows no significant difference when comparing TO, T4w, and T8w. Furthermore, the sum of the relative areas of the third and fourth (high molecular weight species (HMWS)) peaks (FIG. 16B) also shows no significant difference when comparing TO, T4w, and T8w.

[0215] At 25° C., the sum of the relative areas of the first and second (major) peaks (FIG. 17A) shows no significant difference when comparing TO, T2w, T4w, and T8w, and the sum of the relative areas of the third and fourth (HMWS) peaks (FIG. 17B) also shows no significant difference when comparing TO, T2w, T4w, and T8w.

[0216] At 40° C., the sum of the relative areas of the first and second (main) peaks (FIG. 18A) shows no significant difference when comparing TO, T2w, T4w, and T8w, and the sum of the relative areas of the third and fourth (HMWS) peaks (FIG. 18B) also shows no significant difference when comparing TO, T2w, T4w, and T8w.

[0217] For non-reducing capillary electrophoresis sodium dodecyl sulfate (CE-SDS) analysis, samples were diluted to 1 mg / mL in phosphate buffered saline (PBS). The diluted samples were then further diluted 1:1 with 10 mM N-ethylmaleimide (NEM) in PBS. 5.0 μl of the resulting solution was then added to 7.0 μL sample buffer. The samples were then incubated at 70° C. for 10 minutes. After incubation, 32 μl of water was added and the samples were centrifuged at 1200 g for 2 minutes. The samples were then analyzed using a HT High Sensitivity Antibody Analysis 200 using a HT Protein Express LabChip (Perkin Elmer, 760499) with a Protein Express Reagent Kit (Perkin Elmer, CLS960008). Analysis was performed on a LabChip GXII Touch HT (Perkin Elmer).

[0218] At 2-8°C (Figure 19), the purity of the main peak did not show any significant change between T0, T4w, and T8w. At 25°C (Figure 20) and 40°C (Figure 21), the purity of the main peak did not show any significant change between T0, T2w, T4w, and T8w.

[0219] To perform reduced CE-SDS analysis, samples were diluted to 0.5mg / ml in PBS. 5.0μL of diluted sample was added to 7.0μL of sample denaturing solution (24.5μL of 1M dithiothreitol (DTT) added to 700μL Protein Express Sample Buffer). Samples were then incubated at 70°C for 10 minutes. After incubation, 32μL of water was added and samples were centrifuged at 1200g for 2 minutes. Samples were then analyzed using a HT High Sensitivity Antibody Analysis 200 using a HT Protein Express LabChip (Perkin Elmer, 760499) with a Protein Express Reagent Kit (Perkin Elmer, CLS960008). Analysis was performed on a LabChip GXII Touch HT (Perkin Elmer).

[0220] At 2-8°C (Table 9), the purity of peaks 1, 2, 3, and 4 did not show any significant changes between T0, T4w, and T8w. At 25°C (Table 10) and 40°C (Table 11), the purity of peaks 1, 2, 3, and 4 did not show any significant changes between T0, T2w, T4w, and T8w.

[0221] Overall, these results demonstrate that the lyophilized anti-TfR1 Fab-ASO conjugates are stable at all temperatures and time points tested. Table 9. Capillary electrophoresis sodium dodecyl sulfate (reduced) results at 2-8°C (CE-SDS) [Table 9] Table 10. Capillary Electrophoresis Sodium Dodecyl Sulfate (CE-SDS) Results (reduced) at 25°C [Table 10] Table 11. Capillary Electrophoresis Sodium Dodecyl Sulfate (CE-SDS) Results (reduced) at 40°C [Table 11] Additional Aspects 1. A formulation comprising a complex comprising an oligonucleotide covalently linked to an anti-transferrin receptor 1 (TfR1) antibody, wherein the anti-TfR1 antibody comprises the following: heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12, heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13, heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14, light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15, light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11, and light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16; wherein the oligonucleotide comprises a 5'-XYZ-3' configuration, where X and Z are flanking regions comprising one or more modified nucleosides and Y is a gap region comprising one or more 2'-deoxyribonucleosides; and Here the complex is formulated with tris(hydroxymethyl)aminomethane and sucrose. 2.Formula (I):[R 1 ] n1 -R 2 wherein each R 1 are independently represented by formula (Ia): [ka] In the formula R 2 comprises an antibody, and R 3comprises an oligonucleotide comprising a 5'-XYZ-3' configuration, where X and Z are flanking regions comprising one or more modified nucleosides and Y is a gap region comprising one or more 2'-deoxyribonucleosides; R in the formula 1 is R 2 is covalently linked to at attachment point A; and In the formula, n1 is R 1 is an integer representing the number of instances of 1 each instance of is covalently linked to a different amino acid residue of the antibody, optionally where each different amino acid residue is a lysine; Here, the complex is formulated with tris(hydroxymethyl)aminomethane and sucrose, Optionally wherein the antibody is an anti-TfR1 antibody. 3. The formulation of embodiment 2, wherein the antibody comprises: heavy chain complementarity determining region 1 (CDR-H1) comprising the sequence as set forth in SEQ ID NO: 1, 7, or 12; heavy chain complementarity determining region 2 (CDR-H2) comprising the sequence as set forth in SEQ ID NO: 2, 8, or 13; heavy chain complementarity determining region 3 (CDR-H3) comprising the sequence as set forth in SEQ ID NO: 3, 9, or 14; light chain complementarity determining region 1 (CDR-L1) comprising the sequence as set forth in SEQ ID NO: 4, 10, or 15; light chain complementarity determining region 2 (CDR-L2) comprising the sequence as set forth in SEQ ID NO: 5 or 11; and light chain complementarity determining region 3 (CDR-L3) comprising the sequence as set forth in SEQ ID NO: 6 or 16. 4. The formulation of any one of aspects 1-3, wherein the formulation is in lyophilized form. 5. The formulation of any one of aspects 1-3, wherein the formulation is in a frozen solid form. 6. The formulation of any one of aspects 1-3, wherein the formulation is in an aqueous solution. 7. The formulation of embodiment 6, wherein tris(hydroxymethyl)aminomethane is present in the aqueous solution at a concentration ranging from 5 mM to 50 mM. 8. The formulation of embodiment 6 or 7, wherein the sucrose is present in the aqueous solution at a concentration ranging from 5% to 15% weight per volume (w / v%). 9. The formulation of any one of aspects 6-8, wherein the aqueous solution has a pH in the range of 6.5 to 8.5. 10. The formulation of any one of aspects 6-9, wherein the tris(hydroxymethyl)aminomethane is present in a concentration of 25 mM in the aqueous solution, and / or the sucrose is present in a concentration of 10 w / v % in the aqueous solution, and / or the aqueous solution is at a pH of 7.5. 11. The formulation of any one of aspects 1-10, wherein the antibody is a Fab fragment, a full-length IgG, a Fab' fragment, a F(ab')2 fragment, scFv, or Fv. 12. The formulation of embodiment 11, wherein the antibody is a Fab fragment. 13. The antibody comprises a heavy chain variable region (VH) comprising an amino acid sequence at least 85% identical to SEQ ID NO: 17; and / or wherein the antibody comprises a light chain variable region (VL) comprising an amino acid sequence at least 85% identical to SEQ ID NO: 18; The formulation of any one of embodiments 1-12, optionally wherein the antibody comprises a VH comprising the amino acid sequence of SEQ ID NO:17 and / or a VL comprising the amino acid sequence of SEQ ID NO:18. 14. The formulation of any one of aspects 1-13, wherein the antibody comprises a heavy chain comprising an amino acid sequence at least 85% identical to SEQ ID NO: 19; and / or wherein the antibody comprises a light chain comprising an amino acid sequence at least 85% identical to SEQ ID NO: 20, optionally wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and / or comprises a light chain comprising the amino acid sequence of SEQ ID NO: 20. 15. The formulation of any one of aspects 1-14, wherein the oligonucleotide is 10-30 nucleotides in length. 16. The formulation of any one of aspects 1-15, wherein the oligonucleotide comprises a nucleotide sequence having a region of complementarity of at least 8 consecutive nucleotides in length to SEQ ID NO:22. 17. The formulation of any one of aspects 1-16, wherein the oligonucleotide comprises at least 8 contiguous nucleotides of the nucleotide sequence as set forth in SEQ ID NO:21, and optionally the oligonucleotide comprises the base sequence of SEQ ID NO:21. 18. A formulation of any one of aspects 2-17, wherein each R 1 is represented by formula (Ib): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage, such that the oligonucleotide comprises the nucleobase sequence of CAGCGCCCACCAGUCA (SEQ ID NO:21). 19. A formulation of any one of embodiments 2-17, wherein each R 1 is represented by the formula (Ic): [ka] The group represented by the formula: 20. The formulation of any one of aspects 1-19, wherein the complex is present in the formulation at a concentration ranging from 10 mg / mL to 50 mg / mL. 21. The formulation of any one of aspects 1-20, further comprising one or more antibodies that are not covalently linked to the oligonucleotide. 22. The formulation of embodiment 21, wherein the average value of n1 of the complex in the formulation is in the range of 0.5 to 5. 23. A method of reducing DMPK expression in a subject, the method comprising administering to the subject an effective amount of a formulation of any one of aspects 1-22. 24. A method of treating myotonic dystrophy in a subject, the method comprising administering to the subject an effective amount of the formulation of any one of embodiments 1-22. 25. The method of embodiment 23 or embodiment 24, wherein the subject has a disease-associated repeat expansion of a DMPK allele associated with myotonic dystrophy. 26. The method of embodiment 25, wherein the disease-associated repeat sequence comprises a repeat unit of a CTG trinucleotide sequence. 27. The method of any one of aspects 23 to 26, wherein the complex reduces DMPK expression in the subject. 28.Formula (I):[R 1 ] n1 -R 2 wherein each R 1 is represented by formula (Ia): [ka]

[0043] R 3 comprises an oligonucleotide comprising the structure represented by +C*+A*oG*oC*dG*dC*dC*dC*dA*dC*dC*dA*oG*oU*+C*+A (SEQ ID NO:21), where +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-MOE modified ribonucleoside, oC represents a 5-methyl-2'-MOE-cytidine, +C represents a 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents a 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage; Here, R 2 comprises a Fab, and wherein the Fab comprises a CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 selected from Table 2; Optionally, wherein the Fab comprises a VH comprising the amino acid sequence of SEQ ID NO:17 and a VL comprising the amino acid sequence of SEQ ID NO:18; Further optionally, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:19 and a light chain comprising the amino acid sequence of SEQ ID NO:20; Here, R 1 is R 2at attachment point A; and wherein n1 is R 1 is an integer representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of Fab, optionally where each different amino acid residue is a lysine. 29.Formula (I):[R 1 ] n1 -R 2 A complex comprising a structure represented by the formula Each R 1 is represented by formula (Ib): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents a 5-methyl-2'-MOE-cytidine, +C represents a 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents a 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage, such that the oligonucleotide comprises the nucleobase sequence CAGCGCCCACCAGUCA (SEQ ID NO:21); Here, R 2 comprises a Fab, and wherein the Fab comprises a CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 selected from Table 2; Optionally, wherein the Fab comprises a VH comprising the amino acid sequence of SEQ ID NO:17 and a VL comprising the amino acid sequence of SEQ ID NO:18; Further optionally, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:19 and a light chain comprising the amino acid sequence of SEQ ID NO:20; Here, R 1 is R 2 at attachment point A; and wherein n1 is R 1 is an integer representing the number of instances of 1Each instance of is covalently linked to a different amino acid residue of Fab, optionally where each different amino acid residue is a lysine. 30.Formula (I):[R 1 ] n1 -R 2 A complex comprising a structure represented by the formula R 1 is represented by the formula (Ic): [ka]

[0043] R in the formula 2 comprises a Fab comprising a CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 selected from Table 2; Optionally, R in the formula 2 comprises a Fab comprising a VH comprising the amino acid sequence of SEQ ID NO: 17 and a VL comprising the amino acid sequence of SEQ ID NO: 18; Furthermore, optionally, in the formula R 2 comprises a Fab comprising a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 20; 1 is R 2 is covalently linked to at attachment point A; In the formula, n1 is R 1 is an integer representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of Fab, optionally where each different amino acid residue is a lysine. 31. Formula (Id): [ka] wherein +N represents an LNA (2'-4' methylene bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage; R in the formula 2 comprises a Fab, and wherein the Fab comprises a CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3 selected from Table 2; Optionally, wherein the Fab comprises a VH comprising the amino acid sequence of SEQ ID NO:17 and a VL comprising the amino acid sequence of SEQ ID NO:18; Further optionally, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:19 and a light chain comprising the amino acid sequence of SEQ ID NO:20; where n1 is an integer representing the number of instances of the group enclosed by the brackets, where each instance of the group enclosed by the brackets is covalently linked to a different amino acid residue of Fab, optionally where each different amino acid residue is a lysine. 32. A formulation comprising a plurality of conjugates of any one of embodiments 28-31, tris(hydroxymethyl)aminomethane at a concentration of 5-50 mM, and sucrose at a concentration of 5% w / v to 10% w / v, wherein the formulation is an aqueous solution and has a pH of 6.5-8.5, and optionally wherein the plurality of conjugates is at a concentration of 10 mg / mL to 50 mg / mL. 33. The formulation of embodiment 32 in lyophilized form. 34. A product produced by a process comprising lyophilizing the formulation of embodiment 32. 35. The formulation of embodiment 32 in frozen form. 36. A product produced by a process comprising freezing the formulation of embodiment 32. 37. A formulation comprising a plurality of conjugates of any one of embodiments 28-31, tris(hydroxymethyl)aminomethane at a concentration of 5-50 mM, and sucrose at a concentration of 5% w / v to 15% w / v, wherein the formulation is an aqueous solution and has a pH of 6.5-8.5, and optionally wherein the plurality of conjugates is at a concentration of 10 mg / mL to 50 mg / mL. 38. A formulation comprising a plurality of conjugates of any one of embodiments 28-31, tris(hydroxymethyl)aminomethane at a concentration of 25 mM, and sucrose at a concentration of 10 w / v %, wherein the formulation is an aqueous solution and has a pH of 7.5, and optionally wherein the plurality of conjugates is at a concentration of 10 mg / mL to 50 mg / mL. 39. The formulation of any one of embodiments 32, 37, and 38, further comprising one or more antibodies that are not covalently linked to the oligonucleotide. 40. The formulation of embodiment 39, wherein the average value of n1 of the complex in the formulation is in the range of 0.5 to 5. 41. The formulation of any one of embodiments 37-40 in lyophilized form. 42. A lyophilized cake comprising a plurality of conjugates of any one of embodiments 28-31, tris(hydroxymethyl)aminomethane, and sucrose. 4.02x10 per 43.1g cake -2 43. The lyophilized cake of embodiment 42, comprising: 989 mg to 999 mg sucrose per gram of cake; and / or 0.666 mg to 9.90 mg complex per gram of cake; Optionally, the lyophilized cake herein comprises: (i) 0.303 mg tris(hydroxymethyl)aminomethane per gram of cake, 998.6 mg sucrose per gram of cake, and / or 0.999 mg complex per gram of cake; (ii) 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 997.7 mg sucrose per gram of cake, and / or 1.995 mg complex per gram of cake; (iii) 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 997.2 mg sucrose per gram of cake, and / or 2.49 mg complex per gram of cake; (iv) 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 996.7 mg sucrose per gram of cake, and / or 2.99 mg complex per gram of cake; (v) 0.302 mg tris(hydroxymethyl)aminomethane per gram of cake, 995.7 mg sucrose per gram of cake, and / or 3.98 mg complex per gram of cake; or (vi) 0.301 mg tris(hydroxymethyl)aminomethane per gram of cake, 994.7 mg sucrose per gram of cake, and / or 4.97 mg complex per gram of cake. 44. The lyophilized cake of embodiment 42 or embodiment 43, wherein when about 10 g of the lyophilized cake is reconstituted in water to produce 1 mL of solution, the solution contains tris(hydroxymethyl)aminomethane at a concentration of 5 to 50 mM, sucrose at a concentration of 5% to 15% w / v, and the complex at a concentration of 10 mg / mL to 50 mg / mL, optionally wherein tris(hydroxymethyl)aminomethane is at a concentration of 25 mM and / or sucrose is at a concentration of 10% w / v.

[0222] Equivalents and Terminology The present disclosure as illustrated and described herein may be suitably practiced in the absence of any element(s), limitation(s) not specifically disclosed herein. Thus, for example, in each instance of this application, any of the terms "comprise", "consist essentially of" and "consist of" may be replaced with either of the other two terms. The terms and expressions employed are used as terms of description and not of limitation, and the use of such terms and expressions is not intended to exclude any equivalents or portions thereof of the features shown and described, and it is recognized that various modifications are possible within the scope of the present disclosure. Thus, although the present disclosure has been specifically disclosed by preferred embodiments, it should be understood that any features, modifications and variations of the concepts disclosed herein may be resorted to by those skilled in the art, and such modifications and variations are considered to be within the scope of the present disclosure.

[0223] Additionally, where features or aspects of the disclosure are described as a Markush group or other grouping of alternatives, those skilled in the art will recognize that the disclosure is thereby also described as any individual member or subgroup of members of the Markush group or other group.

[0224] It should be understood that in some embodiments, when describing the structure of an oligonucleotide or other nucleic acid, reference may be made to the sequences presented in the sequence listing. In such embodiments, the actual oligonucleotide or other nucleic acid may have one or more alternative nucleotides (e.g., RNA counterparts of DNA nucleotides, or DNA counterparts of RNA nucleotides) and / or (for example and) one or more modified nucleotides and / or (for example and) one or more modified internucleotide linkages and / or (for example and) one or more other modifications compared to the designated sequence, while retaining essentially the same or similar complementary properties as the designated sequence.

[0225] The use of the terms "a" and "an" and "the" and similar referents in the context of describing the present invention (particularly in the context of the claims below) should be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms "comprise", "have", "include", and "contain" should be construed as open-ended terms (i.e., meaning "including, but not limited to"), unless otherwise indicated herein. The recitation of ranges of values ​​herein is merely intended to serve as a shorthand method of individually referring to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated herein as if it were individually recited herein. Unless otherwise indicated, ranges of values ​​herein include their endpoints (for example, a range from X to Y includes values ​​X and Y). It should be understood that the recitation of values ​​from X to Y herein indicates that the specified value is within the range from X to Y. All methods described herein can be carried out in any suitable order unless otherwise indicated herein or clearly contradicted by context.Unless otherwise claimed, the use of any and all examples or exemplary language (e.g., "etc.") provided herein is intended merely to better elucidate the invention and does not impose limitations on the scope of the invention.No language in this specification should be construed as indicating any non-claimed element as essential to the practice of the invention.

[0226] Embodiments of the invention have been described herein. Variations of those embodiments may become apparent to those of skill in the art upon reading the above description.

[0227] The inventors anticipate that those skilled in the art will adopt such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than as specifically described herein. Accordingly, the present invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the present invention unless otherwise indicated herein or otherwise clearly contradicted by context. Those skilled in the art will recognize, or be able to ascertain, at most with routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

1. Formula (I):[R 1 ] n1 -R 2 A complex comprising a structure represented by R 1 is represented by formula (Ic): 【Chemistry 1】 and a group represented by R 2 comprises a Fab, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 20; R 1 is R 2 at attachment point A; and n1 is R 1 is an integer greater than or equal to 1 representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of the Fab. The complex.

2. 2. The conjugate of claim 1, wherein each different amino acid residue is a lysine.

3. 2. The conjugate of claim 1, wherein each different amino acid residue is a cysteine.

4. 2. The conjugate of claim 1, wherein the heavy chain of the Fab comprises an N-terminal pyroglutamate.

5. 2. The conjugate of claim 1, wherein n1 is 1 to 5.

6. Formula (I):[R 1 ] n1 -R 2 A complex comprising a structure represented by Each R 1 is represented by formula (Ia): 【Chemistry 2】 and a group represented by R 2 comprises a Fab, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 20; and R 3 comprises an oligonucleotide comprising the structure +C*+A*oG*oC*dG*dC*dC*dC*dA*dC*dC*dA*oG*oU*+C*+A (SEQ ID NO: 21), For each nucleoside N of the oligonucleotide, +N represents an LNA (2'-4' methylene-bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine, where the 2' and 4' positions of the sugar are bridged via a methylene, oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage; R 1 is R 2 is covalently linked to at attachment point A; and n1 is R 1 is an integer greater than or equal to 1 representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of the Fab. The complex.

7. 7. The conjugate of claim 6, wherein each different amino acid residue is a lysine.

8. 7. The conjugate of claim 6, wherein each different amino acid residue is a cysteine.

9. 7. The conjugate of claim 6, wherein the heavy chain of the Fab comprises an N-terminal pyroglutamate.

10. The conjugate of claim 6, wherein n1 is 1 to 5.

11. Formula (I):[R 1 ] n1 -R 2 A complex comprising a structure represented by Each R 1 is represented by formula (Ib): 【Transformation 3】 and a group represented by R 2 comprises a Fab, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 20; and for each nucleoside N of the oligonucleotide, +N represents an LNA (2'-4' methylene-bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE) modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene-bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage, such that the oligonucleotide comprises the nucleobase sequence CAGCGCCCACCAGUCA (SEQ ID NO: 21); R 1 is R 2 is covalently linked to at attachment point A; and n1 is R 1 is an integer greater than or equal to 1 representing the number of instances of 1 Each instance of is covalently linked to a different amino acid residue of the Fab. The complex.

12. 12. The conjugate of claim 11, wherein each different amino acid residue is a lysine.

13. 12. The conjugate of claim 11, wherein each different amino acid residue is a cysteine.

14. 12. The conjugate of claim 11, wherein the heavy chain of the Fab comprises an N-terminal pyroglutamate.

15. 12. The conjugate of claim 11, wherein n1 is 1 to 5.

16. Formula (Id): 【Chemistry 4】 A complex comprising a structure represented by For each nucleoside N of the oligonucleotide, +N represents an LNA (2'-4' methylene-bridged) ribonucleoside, dN represents a 2'-deoxyribonucleoside, oN represents a 2'-O-methoxyethyl (MOE)-modified ribonucleoside, oC represents 5-methyl-2'-MOE-cytidine, +C represents 5-methyl-2'-4'-bicyclic-cytidine (2'-4' methylene-bridged), oU represents 5-methyl-2'-MOE-uridine, and * represents a phosphorothioate internucleoside linkage, such that the oligonucleotide comprises the nucleobase sequence CAGCGCCCACCAGUCA (SEQ ID NO: 21); R 2 comprises a Fab, wherein the Fab comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 20; n1 is an integer greater than or equal to 1 representing the number of instances of the group enclosed by the square brackets, where each instance of the group enclosed by the square brackets is covalently linked to a different amino acid residue of the Fab; The complex.

17. 17. The conjugate of claim 16, wherein each different amino acid residue is a lysine.

18. 17. The conjugate of claim 16, wherein each different amino acid residue is a cysteine.

19. 17. The conjugate of claim 16, wherein the heavy chain of the Fab comprises an N-terminal pyroglutamate.

20. 17. The conjugate of claim 16, wherein n1 is 1 to 5.

21. A composition comprising the complex of any one of claims 1 to 20, formulated in a manner suitable for pharmaceutical use.

22. 21. A composition comprising a conjugate according to any one of claims 1 to 20 for use in a method of treating myotonic dystrophy in a subject, said method comprising administering said composition to the subject.

23. 23. The composition for use of claim 22, wherein the subject has a disease-associated repeat expansion of a DMPK allele associated with myotonic dystrophy.

24. 24. The composition for use of claim 23, wherein the disease-associated repeat comprises a repeat unit of a CTG trinucleotide sequence.

25. 23. The composition for use according to claim 22, wherein the subject is a human.

26. 21. A composition comprising a complex of any one of claims 1 to 20 for use in a method for reducing DMPK expression, said method comprising administering said composition to a subject.

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