Bicyclononine reagent for cell imaging

JP2025520445A5Pending Publication Date: 2026-06-22THE GENERAL HOSPITAL CORP
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
THE GENERAL HOSPITAL CORP
Filing Date
2023-06-12
Publication Date
2026-06-22

AI Technical Summary

Technical Problem

Existing fluorescence imaging methods for cell samples, particularly from fine needle aspiration, are limited by harsh quenching conditions that damage cells and require lengthy processing times, restricting the number of stains to 4-6, which is insufficient for detailed cancer cell profiling.

Method used

A bicyclononine (BCN)-based clickable fluorophore (FAST probe) enables ultra-fast single-cell cycling by using a fluorophore-based linker with a BCN moiety, allowing rapid quenching and unquenching with tetrazine or azide reagents, facilitating multi-channel imaging of up to 30 markers within an hour.

Benefits of technology

The BCN-based probes provide superior oxidation and photo-stability, accelerating quenching reactions by 1400- to 5000-fold, enabling rapid and stable fluorescence cycling suitable for multiplexed cellular imaging.

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Abstract

The present disclosure provides compounds and methods for preparing antibody conjugates with fluorophores, as well as methods of using these conjugates for cell imaging. In one embodiment, the conjugate can be bound to a quencher to absorb fluorescence from the fluorophore.
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