Anti-gray hair composition, melanin production-promoting composition, and tyrosinase activity-enhancing composition

Abstract

To provide a new anti-gray hair composition, a melanin production-promoting composition and a tyrosinase activity-enhancing composition.SOLUTION: To provide an anti-gray hair composition, a melanin production-promoting composition and a tyrosinase activity-enhancing composition each containing a hamamelis extract or a eucalyptus extract. The anti-gray hair composition, the melanin production-promoting composition and the tyrosinase activity-enhancing composition are preferably further used in combination with a paulownia leaf extract.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to an anti-hair graying composition, more specifically to a topical composition that prevents and / or improves hair graying based on its tyrosinase activity enhancing effect and melanin production promoting effect. [Background technology]

[0002] Hair turns gray when the supply of melanin, a black pigment component, is depleted during hair production. Melanin is produced by tyrosinase in melanocytes (pigment cells) using tyrosine as a starting material. Therefore, it is widely recognized that increasing tyrosinase activity and promoting melanin production most directly contributes to preventing and improving gray hair. As examples of anti-graying compositions based on this mechanism of action, Patent Document 1 discloses a tyrosinase activity promoter, a melanin production promoter, and a cosmetic composition for preventing and improving gray hair, each containing an extract of Asiatic lily (Lilium asiatica), a member of the Liliaceae family. Patent Document 2 also discloses a tyrosinase activity promoter containing one or more active ingredients selected from the group consisting of birch extract containing a water-containing lower alcohol, cinchona extract, linden extract containing a water-containing lower alcohol, aceca extract, and yeast extract.

[0003] On the other hand, witch hazel extract has astringent and soothing effects and is therefore widely used in skin care products. Eucalyptus extract has soothing and hair-growth effects and is therefore used in skin care products and hair growth agents. For example, Patent Document 3 discloses a hair growth agent containing eucalyptus young leaf extract as an active ingredient. Furthermore, paulownia leaf extract is known to have hair-growth effects, and Patent Document 4, for example, discloses a hair growth agent containing paulownia leaf extract as a hair growth and hair-growth promoting ingredient. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2016-121113 [Patent Document 2] Japanese Patent Application Laid-Open No. 2010-31056 [Patent Document 3] Japanese Patent Application Laid-Open No. 2011-132182 [Patent Document 4] Patent No. 3357746 specification Summary of the Invention [Problem to be solved by the invention]

[0005] The present disclosure aims to solve the above problems and provide a novel anti-hair graying composition, a novel melanin production-promoting composition, and a novel tyrosinase activity-enhancing composition. [Means for solving the problem]

[0006] The present disclosure provides an anti-hair graying composition comprising witch hazel extract or eucalyptus extract as an active ingredient. The present disclosure also provides a melanin production-promoting composition containing witch hazel extract or eucalyptus extract as an active ingredient. The present disclosure further provides a tyrosinase activity enhancing composition comprising a witch hazel extract or a eucalyptus extract as an active ingredient. [Effects of the Invention]

[0007] The present disclosure provides novel anti-hair-graying compositions, melanin production-promoting compositions, and tyrosinase activity-enhancing compositions. [Brief explanation of the drawings]

[0008] [Figure 1] FIG. 1 shows the tyrosinase activity enhancing effect of witch hazel extract (A) and eucalyptus extract (B). [Figure 2A] FIG. 1 is a graph showing the effect of enhancing tyrosinase activity when paulownia leaf extract and witch hazel extract are used in combination. [Figure 2B] FIG. 1 is a graph showing the effect of enhancing tyrosinase activity when paulownia leaf extract and eucalyptus extract are used in combination. DETAILED DESCRIPTION OF THE INVENTION

[0009] Hereinafter, embodiments of the present disclosure will be described in detail. The tyrosinase activity enhancing composition according to this embodiment contains witch hazel extract or eucalyptus extract as an active ingredient. The melanin production-promoting composition according to this embodiment contains witch hazel extract or eucalyptus extract as an active ingredient.

[0010] [Witch hazel extract] Witch hazel (scientific name: Hamamelis virginiana L.), also known as witch hazel, is a plant belonging to the Hamamelidaceae family. Witch hazel extract is an extract obtained by extracting witch hazel leaves or leaves and bark with various extraction solvents. The extraction solvent may be any solvent commonly used for plant extraction. The extraction solvent may be, for example, water; hydrophilic solvents such as methanol, ethanol, glycerin, monohydric or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol; esters such as acetone and ethyl acetate; hydrophobic solvents such as hexane and octanol; or a mixture of these. Among these, one or a mixture of two or more solvents selected from water, ethanol, propylene glycol, and 1,3-butylene glycol is preferred as the extraction solvent. The extraction method may be any method used to produce plant extracts. For example, witch hazel extract can be produced by immersing leaves or leaves and bark in an extraction solvent, removing the leaves, preferably by filtration, and then concentrating the extract as needed. The extraction conditions (extraction temperature, extraction time, etc.) can be appropriately determined by those skilled in the art. In this embodiment, the extract obtained after extraction may be used as is as the witch hazel extract, or may be concentrated or powdered by an appropriate method, or may be purified by bleaching, deodorizing, desalting, etc., within a range that does not impair the tyrosinase activity enhancing effect or melanin production promoting effect, and then used as the witch hazel extract. In addition, the witch hazel extract may be used without removing the leaves, etc.

[0011] [Eucalyptus extract] Eucalyptus (English name: Southern blue gum, scientific name: Eucalyptus globulus Labill.), also known as eucalyptus tree, is a plant belonging to the genus Eucalyptus in the family Myrtaceae. Furthermore, in this specification, closely related plants belonging to the family Myrtaceae other than the narrowly defined eucalyptus (Eucalyptus globulus Labill.) are also referred to as "eucalyptus." Such closely related plants may be, for example, plants belonging to the genera Eucalyptus, Corymbia, and Angophora, but are not particularly limited to these.

[0012] Eucalyptus extract is an extract obtained by extracting eucalyptus leaves with various extraction solvents. The extraction solvent may be any solvent commonly used for plant extraction. Examples of the extraction solvent include water; monohydric or polyhydric alcohols such as methanol, ethanol, glycerin, propylene glycol, and 1,3-butylene glycol; hydrophilic solvents such as esters such as acetone and ethyl acetate; hydrophobic solvents such as hexane and octanol; and mixtures of these solvents. Among these, one or a mixture of two or more solvents selected from water, ethanol, propylene glycol, and 1,3-butylene glycol is preferred. The extraction method may be any method commonly used for producing plant extracts. For example, eucalyptus extract can be produced by immersing leaves in the extraction solvent, removing the leaves, preferably by filtration, and then concentrating the extract as needed. Extraction conditions (e.g., extraction temperature and extraction time) can be determined appropriately by those skilled in the art. In this embodiment, the extract obtained after extraction may be used as is as the eucalyptus extract, or it may be concentrated or powdered by an appropriate method, or may be purified by bleaching, deodorizing, desalting, or other methods to the extent that the tyrosinase activity enhancing effect or melanin production promoting effect is not impaired, and then used as the eucalyptus extract. The eucalyptus extract may also be used without removing the leaves.

[0013] [Paulownia leaf extract] The tyrosinase activity enhancing composition and melanin production promoting composition according to this embodiment may each further contain paulownia leaf extract as an active ingredient. Paulownia leaves are the leaves of Paulownia tomentosa, a plant belonging to the genus Paulownia in the family Thunbaceae. Paulownia leaf extract is an extract obtained by extracting paulownia leaves with various extraction solvents. The extraction solvent may be any solvent commonly used for plant extraction. Examples of the extraction solvent include hydrophilic solvents such as water, monohydric or polyhydric alcohols such as methanol, ethanol, glycerin, propylene glycol, and 1,3-butylene glycol, and esters such as acetone and ethyl acetate, as well as hydrophobic solvents such as hexane and octanol. The extraction solvent may be one or a mixture of two or more solvents, preferably hydrophilic solvents. The extraction method may be any method used for producing plant extracts. For example, the extract may be produced by immersing leaves in the extraction solvent, removing the leaves, preferably by filtration, and then concentrating the extract as needed. The extraction conditions (extraction temperature, extraction time, etc.) can be determined appropriately by those skilled in the art. In this embodiment, after extraction, the extract may be used as is as the paulownia leaf extract, or may be concentrated or powdered by an appropriate method, or may be purified by bleaching, deodorizing, desalting, or other methods to the extent that the tyrosinase activity enhancing effect or melanin production promoting effect is not impaired.The paulownia leaf extract may also be used without removing the leaves.

[0014] [Tyrosinase activity enhancing composition, melanin production promoting composition] The witch hazel extract or eucalyptus extract obtained by the above-described method each exhibits excellent tyrosinase activity enhancing effects, and can therefore be used as is as the tyrosinase activity enhancing composition and melanin production promoting composition according to this embodiment. They can also be mixed with a suitable carrier and used as the tyrosinase activity enhancing composition and melanin production promoting composition. The carrier is a component necessary for formulation, and examples include, but are not limited to, liquids such as water and ethanol, and so-called excipients such as starch and lactose. Furthermore, witch hazel leaves or the leaves and bark themselves, or eucalyptus leaves themselves, can also be used as the tyrosinase activity enhancing composition and melanin production promoting composition.

[0015] The paulownia leaf extract obtained by the above-mentioned method can be mixed with the witch hazel extract or eucalyptus extract to form a tyrosinase activity enhancing composition or a melanin production promoting composition. While the witch hazel extract or eucalyptus extract alone exhibits the effects of enhancing tyrosinase activity and promoting melanin production, their combined use with the paulownia leaf extract produces significant tyrosinase activity enhancing and melanin production promoting effects. The mixing ratio of the witch hazel extract or eucalyptus extract to the paulownia leaf extract can be appropriately determined by those skilled in the art within a range that achieves a synergistic effect of enhancing tyrosinase activity or promoting melanin production. The mixing ratio of the paulownia leaf extract to the witch hazel extract can be, for example, (paulownia leaf extract: witch hazel extract) = 1:1 / 1000 to 100, preferably about 1:1 / 100 to 2, in terms of dry mass ratio. The mixing ratio of paulownia leaf extract to eucalyptus extract can be, for example, (paulownia leaf extract:eucalyptus extract)=1:1 / 1000-100, preferably about 1:1 / 100-2, in terms of dry mass ratio.

[0016] [Anti-graying composition] The anti-hair-graying composition according to this embodiment contains the aforementioned witch hazel extract or eucalyptus extract as an active ingredient. The anti-hair-graying composition according to this embodiment can further contain the aforementioned paulownia leaf extract as an active ingredient. The anti-hair-graying composition promotes melanin production to prevent or improve hair graying. This anti-hair-graying composition is primarily applied to the scalp, but can also be applied to areas where gray hair grows, such as the skin around the mouth where beards grow, arms, and shins.

[0017] The formulation of the anti-hair-graying composition is not particularly limited as long as it is applicable to the scalp or skin other than the scalp, and may be, for example, a liquid, cream, wax, or foam. Furthermore, the anti-hair-graying composition may fall into any of the categories of pharmaceuticals, quasi-drugs, or cosmetics, such as shampoos, rinses, treatments, and hair growth agents. However, the tyrosinase activity-enhancing composition or melanin production-promoting composition according to this embodiment can be applied directly to the scalp or other skin, or mixed with a solvent such as water and applied to the scalp or other skin.

[0018] The content of the witch hazel extract or eucalyptus extract in the anti-hair-graying composition can be an effective amount, and can be appropriately determined by those skilled in the art within a range that can be expected to achieve anti-hair-graying effects. The content varies depending on the formulation and application site, but is, for example, 0.0001 w / w% to 10.0 w / w% and preferably 0.001 w / w% to 2.0 w / w% of the composition as a witch hazel extract dry powder. Furthermore, the content of the eucalyptus extract dry powder is, for example, 0.0001 w / w% to 10.0 w / w% and preferably 0.001 w / w% to 2.0 w / w% of the composition.

[0019] The anti-hair-graying composition according to this embodiment may contain paulownia leaf extract as an active ingredient in addition to the witch hazel extract or eucalyptus extract. The addition of paulownia leaf extract enhances the anti-hair-graying effect, resulting in a more effective anti-hair-graying effect. The mixing ratio of the witch hazel extract or eucalyptus extract to the paulownia leaf extract in the anti-hair-graying composition can be appropriately determined by those skilled in the art within a range that provides a synergistic anti-hair-graying effect. The mixing ratio of the paulownia leaf extract to the witch hazel extract, in terms of dry mass ratio, can be, for example, (paulownia leaf extract: witch hazel extract) = 1:1 / 1000 to 100, preferably about 1:1 / 100 to 2. The mixing ratio of the paulownia leaf extract to the eucalyptus extract can be, for example, (paulownia leaf extract: eucalyptus extract) = 1:1 / 1000 to 100, preferably about 1:1 / 100 to 2.

[0020] In addition to the active ingredients described above, the anti-hair-graying composition according to this embodiment may contain various additives used in typical anti-hair-graying compositions and scalp and hair cosmetics, as well as other active ingredients such as plant extracts, depending on the purpose, within the scope of the effects of the present disclosure. For example, water, excipients, moisturizers, anti-inflammatory agents, antibacterial agents, local irritants, antiseborrheic agents, antioxidants, sequestering agents, surfactants, coenzymes, pH adjusters, thickeners, gelling agents, keratolytic agents, fragrances, pigments, UV absorbers, oily components, vitamins, hormones, and preservatives may be blended. [Example]

[0021] The present disclosure will be explained below using test examples, but the scope of the present disclosure is not limited to these.

[0022] <Test Example 1> Tyrosinase activity enhancing effect of witch hazel extract and eucalyptus extract The witch hazel extract used was a commercially available product (extracted from dried witch hazel (Hamamelis virginiana L.) leaves with an aqueous 1,3-butylene glycol solution). The eucalyptus extract used was also a commercially available product (extracted from dried eucalyptus (Eucalyptus globulus Labill.) or related plant (Myrtaceae) leaves with an aqueous ethanol solution). The tyrosinase activity, which is responsible for the melanin production reaction, was measured for these extracts.

[0023] That is, 2.5 × 10 normal human melanocytes 4 Cells were seeded at 1000 cells / well (96-well plate) and cultured in growth medium. The medium was then replaced with supplement-free medium and cultured for 6 hours. The medium was then replaced with supplement-free medium supplemented with either witch hazel extract or eucalyptus extract at final extract solids concentrations of 0.94 μg / mL, 1.88 μg / mL, 3.75 μg / mL, 7.50 μg / mL, or 15.00 μg / mL, and cultured for 72 hours. Thirty minutes before the end of culture, a nuclear staining dye (Hoechst 33342) was added, and after culture, fluorescence intensity was measured (excitation wavelength 365 nm / emission wavelength 450 nm) to assess the cell number in each well.

[0024] After incubation, 20 μL of lysis buffer was added to each well of the 96-well plate, and the medium was removed. The cells were lysed using a plate mixer to obtain a crude enzyme solution. Next, 100 μL of tyrosinase activity assay reagent (a 2:1:1 mixture of 100 mM sodium phosphate buffer (pH 6.8), 5 mM L-dopa solution, and 20.7 mM 3-methyl-2-benzothiazolinone hydrazone solution) was applied to the crude enzyme solution at 100 μL / well. The reaction was incubated at 37°C for 30 minutes, and the absorbance (492 nm) was measured. The tyrosinase activity was calculated by dividing the absorbance (apparent tyrosinase activity) by the fluorescence intensity (correlated with cell number) measured after nuclear staining.

[0025] The measurement results are shown in Figure 1. In Figure 1, A shows the relative tyrosinase activity values ​​(relative values ​​with the tyrosinase activity value in the control (no extract added) set to 1) at each concentration of witch hazel extract and B shows the relative tyrosinase activity values ​​(relative values ​​with the tyrosinase activity value in the control (no extract added) set to 1) at each concentration of eucalyptus extract. Values ​​are mean ± SD, and ** indicates a significant difference from the control (p<0.01, One-way ANOVA Tukey's test). Treatment with 3.75-15.00 μg / mL of witch hazel extract and 15 μg / mL of eucalyptus extract showed a significant enhancing effect on tyrosinase activity in normal human melanocytes, suggesting that the promotion of melanin production provides an anti-graying effect (Figure 1).

[0026] <Test Example 2> Tyrosinase activity enhancing effect of combined use of witch hazel extract or eucalyptus extract and paulownia leaf extract The effect on tyrosinase activity of the combined use of Hamamelis extract or Eucalyptus extract with Paulownia leaf extract was evaluated. Paulownia leaf extract was obtained by extracting dried Paulownia leaves with warm water, filtering the extract to remove the leaves, and concentrating the extract under reduced pressure. The Hamamelis extract and Eucalyptus extract used were the same as those in Test Example 1. For these extract combinations, tyrosinase activity was measured in the same manner as in Test Example 1.

[0027] That is, 2.5 × 10 normal human melanocytes 4 Cells were seeded at 1000 cells / well (96-well plate) and cultured in growth medium, then replaced with supplement-free medium and cultured for 6 hours. The medium was then replaced with supplement-free medium supplemented with witch hazel extract, eucalyptus extract, and paulownia leaf extract at the solid extract content (final concentration) shown in Figure 2A and B, and cultured for 72 hours. After culture was completed, tyrosinase activity was measured, and the results are shown in Figure 2A and B.

[0028] Figure 2A shows the relative tyrosinase activity values ​​at each concentration when witch hazel extract and paulownia leaf extract were used in combination (relative values ​​with the tyrosinase activity value in the control (no extract added) set to 1). Figure 2B shows the relative tyrosinase activity values ​​at each concentration when eucalyptus extract and paulownia leaf extract were used in combination (relative values ​​with the tyrosinase activity value in the control (no extract added) set to 1). In each figure, values ​​are mean ± SD, ** and * indicate significant differences from the control (p<0.01 and p<0.05, One-way ANOVA Tukey's test), and ## indicates significant differences from the group to which only paulownia leaf extract (10 μg / mL) was added (p<0.01, One-way ANOVA Tukey's test).

[0029] Treatment with paulownia leaf extract alone at 1.00-20.00 μg / mL also demonstrated a significant enhancement of tyrosinase activity in normal human melanocytes. Adding 7.5-15 μg / mL of hamamelis extract (a dry mass ratio of 0.75-1.5 of hamamelis extract to 1 of paulownia leaf extract) to 10 μg / mL of paulownia leaf extract, and adding 3.75-15 μg / mL of eucalyptus extract (a dry mass ratio of 0.375-1.5 of eucalyptus extract to 1 of paulownia leaf extract) to 10 μg / mL of paulownia leaf extract, both demonstrated a greater enhancement of tyrosinase activity than paulownia leaf extract alone (Figures 2A and B). These results suggest that the combined use of hamamelis extract or eucalyptus extract with paulownia leaf extract may further enhance the anti-graying effect.

[0030] Although the embodiments and examples of the present disclosure have been described in detail above, the specific configurations are not limited to these, and design changes that do not deviate from the gist of the present disclosure are included in the present disclosure. [Industrial Applicability]

[0031] The present disclosure provides novel compositions for preventing hair graying, compositions for promoting melanin production, and compositions for enhancing tyrosinase activity, and is therefore expected to contribute to the beauty industry, cosmetics manufacturing industry, and the like.

Claims

1. An anti-graying composition comprising witch hazel extract or eucalyptus extract.

2. The anti-hair graying composition according to claim 1, further comprising paulownia leaf extract.

3. A melanin production-promoting composition containing witch hazel extract or eucalyptus extract.

4. The melanin production-promoting composition according to claim 3, further comprising paulownia leaf extract.

5. A composition for enhancing tyrosinase activity, comprising witch hazel extract or eucalyptus extract.

6. The tyrosinase activity enhancing composition according to claim 5, further comprising paulownia leaf extract.

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