Oral Composition

Abstract

To provide an oral composition having the effect of maintaining and strengthening muscle tissue. SOLUTION: The oral composition is characterized by containing kale and at least one other ingredient selected from protein, mulberry, and sucralose.

Description

[Technical Field]

[0001] The present invention relates to an oral composition containing kale. [Background technology]

[0002] BACKGROUND ART In recent years, with the background of increasing interest in health, various health foods have been developed, and research into various materials to be used in health foods has been conducted.

[0003] For example, kale is a type of plant in the Brassicaceae family, the same family as cabbage, and is rich in vitamins such as vitamin C, minerals, dietary fiber, chlorophyll, etc. It is also effective in preventing gastritis and gastric ulcers, improving liver function, and relieving constipation, and is therefore attracting attention as a health food suitable for maintaining human health (see, for example, Patent Document 1).

[0004] In addition, in today's world where people are as safety-conscious as they are health-conscious, products made from safe ingredients such as kale, which is eaten on a daily basis, are preferred, and there is a desire to make better use of the useful functions of kale, which is a commonly eaten vegetable. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Laid-Open No. 2002-171932 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a kale-containing oral composition that has the effect of maintaining and strengthening muscle tissue. [Means for solving the problem]

[0007] The present inventors discovered that the use of specific ingredients (other ingredients) together with kale can provide an excellent myoblast proliferation effect, leading to the completion of the present invention.

[0008] That is, the present invention is as follows. [1] An oral composition characterized by containing kale and at least one other ingredient selected from protein, gymnema, sucralose, mulberry, and bracken. [2] The oral composition according to [1] above, characterized in that it contains at least two other ingredients. [3] The oral composition according to [1] or [2] above, characterized in that it is used for dieting. [4] The oral composition according to any one of [1] to [3] above, characterized by containing dried powder of kale. [5] The oral composition according to [4] above, wherein the dried kale powder is a dried powder obtained by crushing and powdering dried green leaves of kale. [Effects of the Invention]

[0009] The oral composition of the present invention has excellent effects of maintaining and strengthening muscle tissue. [Brief explanation of the drawings]

[0010] [Figure 1] 1 is a graph showing the results of a dispersion test of a composition of the present invention (kale + Japanese bamboo (+ other materials other than Japanese bamboo)). [Figure 2] 1 is a graph showing the results of a dispersion test of a composition of the present invention (kale + mulberry leaves (+ other materials other than mulberry leaves)). [Figure 3] 1 is a graph showing the results of a dispersion test of a composition of the present invention (kale + protein (+ other ingredients other than protein)). [Figure 4] 1 is a graph showing the results of a dispersion test of a composition of the present invention (kale + gymnema (+ other material other than gymnema)). [Figure 5] 1 is a graph showing the results of a dispersion test of a composition of the present invention (kale + sucralose (+ other ingredients other than sucralose)). DETAILED DESCRIPTION OF THE INVENTION

[0011] The oral composition of the present invention is characterized by containing kale and at least one other ingredient selected from protein, gymnema, sucralose, mulberry, and kumazasa.

[0012] The oral composition of the present invention has an excellent myoblast proliferation effect and can maintain and strengthen muscle tissue. Furthermore, since muscle tissue is a tissue with active energy metabolism, maintaining and strengthening this muscle tissue maintains and increases basal metabolic rate, which in turn can provide a diet effect by preventing and / or eliminating obesity.

[0013] Furthermore, oral compositions containing kale of the present invention and at least one other ingredient have higher dispersibility than kale alone. That is, the oral compositions of the present invention are powders (powder) that are less likely to aggregate. This highly dispersible oral composition can be prepared simply by mixing, and is easy to prepare. For example, when mixing ingredients that are highly cohesive and prone to clumping, it is known that the ingredients must be pretreated, such as by crushing them using a grinder, to ensure the flowability of the ingredients before being charged, and that after charging, aeration must be performed as quickly as possible to prevent re-agglomeration and initiate flow. However, this composition does not require such cumbersome operations. It is also known that premixing ingredients that are highly cohesive and prone to clumping with an excipient can sometimes reduce adhesion, but such additions are not necessary.

[0014] Furthermore, since the composition of the present invention containing kale and at least one other ingredient has high dispersibility, even when ingredients other than kale and the other ingredients are mixed to form the oral composition of the present invention, the above-mentioned complicated operations are not required, and the composition can be prepared simply by mixing.

[0015] [Kale] Kale is a plant of the Brassicaceae family. The variety of kale used in the present invention is not particularly limited, and various types of kale can be used, such as kitchen kale, tree kale, bush kale, marrow kale, collard greens, and green-leaf kale. It is preferable that the kale be processed, such as dried and crushed, immediately after harvest. If time is required before processing, it is preferable to store the kale using a storage method commonly used by those skilled in the art, such as low-temperature storage, to prevent deterioration. The part of the kale used in the present invention is preferably the leaves, and among the leaves, green leaves are preferred. Furthermore, the kale may also include stems together with the leaves.

[0016] Kale can be used as a pulverized product, squeezed juice, extract, etc. Examples of pulverized products include dried powder, shredded product, and dried products thereof (dried shredded product). The squeezed juice and extract may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. As for kale, dried green leaves of kale are preferred, shredded products and dried products thereof (dried shredded products) are more preferred, and dried powder obtained by pulverizing and powdering is particularly preferred, since the nutritional components contained in kale can be effectively utilized. Furthermore, the dried powder of kale preferably contains 60% or more, more preferably 75% or more, and particularly preferably 90% or more of particles with a particle size of 75 μm or less.

[0017] [Other materials] The other ingredient used together with kale is at least one selected from protein, gymnema, sucralose, mulberry, and kumazasa, and it is preferable to use a combination of at least two of these other ingredients. When two or more other ingredients are combined, a combination of protein and mulberry, or a combination of gymnema and sucralose is particularly preferable. That is, oral compositions containing kale, protein, and mulberry, and oral compositions containing kale, gymnema, and sucralose are preferred because they exhibit a particularly excellent myoblast proliferation effect due to the synergistic action of the combination, and can be expected to have a particularly excellent diet (anti-obesity) effect.

[0018] (protein) The protein is extracted and purified from proteins contained in animals or plants, and commercially available products can be used. In the present invention, powdered proteins are preferred. Examples of animal-derived proteins include whey protein, casein protein, egg protein, and beef protein, while examples of plant-derived proteins include soy protein, pea protein, and rice protein. In the present invention, it is preferable to use plant-derived proteins, and soybean-derived proteins (soy protein) are more preferred, as they exhibit a particularly excellent myoblast proliferation effect when combined with kale. Furthermore, soy protein with a protein content of 50% or more, preferably 70% or more, and particularly preferably 90% or more, on a dry matter basis, exhibits a particularly excellent dispersibility-enhancing effect when combined with kale.

[0019] (Gymnema) Gymnema is a vine belonging to the Apocynaceae family native to India and Sri Lanka, and examples thereof include Gymnema sylvestre. In the present invention, the leaves are preferred. The stems may also be included together with the leaves. Gymnema can be used as a pulverized product, a squeezed juice, an extract, or the like. Examples of pulverized products include a dried powder, a shredded product, and a dried product thereof (dried shredded product). The squeezed juice or extract may be in a liquid form, or can also be used as a paste or a dried powder (juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent include water (hot water), ethanol, and aqueous ethanol. In the present invention, a dried powder of gymnema is preferred, and a dried powder of a hydroalcoholic extract of gymnema leaves is more preferred because it exhibits a particularly excellent myoblast proliferation effect when combined with kale. Furthermore, since the dried powder of gymnema leaves exhibits particularly excellent dispersibility-improving effects when combined with kale, it is preferable that the gymnemic acid content be 5 to 80%, more preferably 10 to 60%, and particularly preferably 20 to 40%.

[0020] (sucralose) Sucralose is a safe, non-caloric, high-intensity sweetener produced from sucrose and having a sweetness approximately 600 times that of sucrose. In the present invention, various forms such as liquid, powder, and solid can be used, and commercially available products can be used. In the present invention, powder is preferred, and since the combination with kale exhibits a particularly excellent effect of improving dispersibility, it is preferred that 80% or more, more preferably 90% or more, and particularly preferably 99% or more of the particles have a particle size of 20 mesh or less.

[0021] (mulberry) Mulberry is a plant of the family Moraceae. The part used in the present invention is preferably the leaves, and among the leaves, green leaves are preferred. The leaves may also contain stems. Mulberry can be used as a pulverized product, squeezed juice, extract, etc. Examples of pulverized products include dried powder, shredded products, and dried products thereof (dried shredded products). The squeezed juice and extract may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). Extracts can be obtained by extraction using an appropriate solvent, such as water (hot water), ethanol, or aqueous ethanol. In the present invention, dried mulberry powder is preferred, and since its combination with kale exhibits a particularly excellent myoblast proliferation effect, it is more preferable to use dried powder obtained by crushing and powdering dried green mulberry leaves. Furthermore, since its combination with kale exhibits a particularly excellent dispersibility-improving effect, it is preferable that 60% or more, more preferably 75% or more, and particularly preferably 90% or more of the dried mulberry powder have a particle size of 75 μm or less.

[0022] (Kumazasa) Kumazasa (Japanese bamboo grass) is a grass family plant. In the present invention, leaves are preferred, with green leaves being particularly preferred. Stems may also be included along with the leaves. Kumazasa can be used as a pulverized product, squeezed juice, extract, etc. Examples of pulverized products include dried powder, shredded products, and dried products (dried shredded products). Juice and extracts may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). Extracts can be obtained by extraction using an appropriate solvent, and examples of solvents that can be used include water (hot water), ethanol, and aqueous ethanol. In the present invention, dried powder of Kumazasa is preferred, and since combining it with kale exhibits particularly excellent myoblast proliferation effects, it is more preferred to use dried powder obtained by pulverizing and powdering dried Kumazasa green leaves. Furthermore, since combining it with kale provides a particularly excellent effect in improving dispersibility, it is preferable that the dry powder of Japanese bamboo be 50% or more of particles of 350 mesh or less, more preferably 75% or more, and particularly preferably 90% or more.

[0023] The oral composition of the present invention has a diet (anti-obesity) effect based on its excellent myoblast proliferation activity, and can therefore be used as a so-called health food, such as a functional food whose efficacy has been approved by a designated organization, such as a food for specified health uses, a food with nutrient function claims, or a food with functional claims, or as a pharmaceutical product (including quasi-drugs), in addition to general foods, food additives, feed, etc.

[0024] That is, the oral composition of the present invention can be used as an oral diet composition for dieting, such as so-called health foods and pharmaceuticals. Such oral diet compositions are not particularly limited as long as they contain kale and specified ingredients (other ingredients) and can be distinguished from other products in that they are used for dieting. For example, the scope of the present invention includes products that display a diet function on the product itself, packaging, instructions, or promotional materials of the present invention. Note that the oral diet composition of the present invention is not limited to products that display the combined ingredients of the present invention (kale and other ingredients) as the active ingredient for dieting on the product packaging, etc. For example, the active ingredient may not be specified, and the active ingredient may be listed as kale alone.

[0025] Specifically, examples of so-called health foods include those labeled as "for those concerned about weight," "boosting metabolism," "helping obese people reduce body fat," "boosting the ability to metabolize fat," and "supporting a slim figure."

[0026] Examples of the form of the composition of the present invention include tablets, capsules, powders, granules, liquids, particles, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, chewable tablets, and sticks. Among these, tablets, capsules, powders, granules, and liquids are particularly preferred. Specific examples include supplements, packaged beverages filled in PET bottles, cans, jars, etc., instant beverages (powdered beverages) to be dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives. These are preferred because they are easy to drink during meals and can enhance palatability.

[0027] The content of kale and other ingredients (components of the present invention) in the oral composition of the present invention may be appropriately determined within a range that provides the desired effect.

[0028] Generally, when the oral composition of the present invention is a supplement or pharmaceutical product in the form of a tablet or other tablet, the components of the present invention are preferably contained in an amount of 0.01 to 100% by mass, more preferably 0.1 to 80% by mass, and even more preferably 1 to 60% by mass, of the total composition in dry mass terms.

[0029] When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.001 to 30% by mass, more preferably 0.01 to 20% by mass, and even more preferably 0.1 to 15% by mass of the total composition.

[0030] Furthermore, when the oral composition of the present invention is an instant drink such as green juice, the component of the present invention is contained in an amount of preferably 0.01 to 100% by mass, more preferably 0.1 to 80% by mass, and even more preferably 1 to 60% by mass, of the total composition converted into dry mass.

[0031] In order to more effectively exert the effects of the present invention, the components of the present invention are preferably contained in an amount of 70% or more, more preferably 80% or more, even more preferably 90% or more, and particularly preferably 100% of the total oral composition of the present invention, calculated on a dry mass basis.

[0032] The intake amount of the oral composition of the present invention is not particularly limited, but from the viewpoint of more significantly exhibiting the effects of the present invention, the daily intake amount of kale per adult is preferably 0.01 g / day or more, more preferably 0.1 g / day or more, and even more preferably 1 g / day or more, calculated on a dry mass basis. The upper limit is, for example, 30 g / day, preferably 20 g / day, and more preferably 10 g / day. The oral composition of the present invention can be stored as a daily amount in a single container or divided into, for example, 2 to 3 containers so that the daily intake amount is the above-mentioned intake amount.

[0033] The blending mass ratio of kale to other ingredients is preferably 0.0001:1 to 10000:1, more preferably 0.001:1 to 1000:1, and even more preferably 0.01:1 to 100:1, calculated on a dry mass basis.

[0034] The oral composition of the present invention can be prepared by known methods, if necessary, by adding ingredients other than the ingredients of the present invention. Examples of ingredients other than the ingredients of the present invention include vitamins such as water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, and vitamin P) and oil-soluble vitamins (vitamins A, D, E, and K); minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds contained in taurine and garlic; flavanoids or flavonoids such as hesperidin and quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; and ground products or extracts of animals and plants (other than kale and the other ingredients of the present invention).

[0035] The oral composition of the present invention exhibits the myoblast proliferation-promoting effect shown in the Examples below, making it an excellent composition for myoblast proliferation, muscle building, muscle mass increase, energy metabolism activator, diet composition, adipose tissue reduction, and body fat reduction. Furthermore, the dispersibility-improving effect shown in the Examples below makes it an excellent oral composition that is less likely to aggregate and highly dispersible. [Example]

[0036] The present invention will be described below based on examples. [Example 1] (raw materials) The kale used was dried kale powder (90% or more of which had particle sizes of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.), which was made by drying and powdering kale leaves. The Kumazasa plant used was dried Kumazasa powder (commercially available product) made by drying and powdering Kumazasa leaves (90% or more of which were 350 mesh or smaller). As the mulberry leaves, dried mulberry leaves were powdered to form dried mulberry leaf powder (90% or more of which had particle sizes of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.). The protein used was powdered isolated soy protein (commercially available, with a protein content of 90% or more on a dry matter basis) obtained by neutralizing the curd obtained by acid precipitation of soy milk extracted from defatted soybeans, drying it, and passing it through a 20-mesh sieve. Gymnema sylvestre powder (commercially available) was used as gymnema extract, which was extracted from Gymnema sylvestre leaves with aqueous ethanol, dried, and powdered (Gymnema sylvestre powder; 40 mesh or less is 90% or more, 80 mesh or less is 85% or more, gymnemic acid content 25-30%; commercial product).Sucralose powder was obtained by sieving powdered sucralose through a 20-mesh sieve.

[0037] These raw materials were blended at the test substance concentrations (μg / mL) shown in Table 1 and at the blending ratios shown in Table 2, and each test was carried out.

[0038] [Test Example 1] (Cell proliferation test) Mouse myoblast cells (C2C12) were cultured in 10% FBS-DMEM in a 75cm flask at 37°C in a 5% CO2 incubator. The cells were suspended by trypsinization and seeded from the 75cm2 flask into each well of a collagen-coated 96-well plate at a cell density of 8000 cells per well.

[0039] After pre-incubation for 24 hours in a 37°C, 5% CO 2 incubator, 100 μL of a test substance prepared in 10% FBS-DMEM was added to the wells. Specifically, the test substance was prepared and added as follows. First, the test substance was suspended in 10% FBS-DMEM to a concentration of 5 mg / ml and filtered through a 0.2 μm filter. Next, this 5 mg / ml suspension of the test substance was further diluted with 10% FBS-DMEM and added to the wells to the concentrations shown in Table 1.

[0040] After 24 hours of incubation, the cells were washed twice with PBS, and 100 μL of filter-sterilized MTT solution (MTT 500 μg / mL in DMEM) was added. The cells were then incubated for another 3 hours. After washing twice with PBS, 100 μL of 0.04% HCl in isopropanol was added.

[0041] After confirming that the dye had been released from the cells, the absorbance (570 nm, 630 nm) was measured to determine the cell proliferation rate (% of control).

[0042] % of control =[SampleDate] / [ControlDate]×100

[0043] Data =[(Sample570nm-Sample630nm)-(blank570nm-blank630nm)]

[0044] Sample 570nm and blank 570nm are absorbance values ​​at 570nm, and Sample 630nm and blank 630nm are absorbance values ​​at 630nm (turbidity).

[0045] The results are shown in Table 1.

[0046] [Table 1]

[0047] As shown in Table 1, the compositions of the present invention containing kale and at least one other ingredient showed an increase in cell proliferation rate greater than the theoretical value expected from the composition alone (calculated based on the blending ratio using the values ​​from the comparative example). In other words, a synergistic increase was observed due to the combination of kale and other ingredients. In particular, when two other ingredients were combined with kale, the increase was more significant than when only one other ingredient was combined. In particular, Example 7, which contained kale, mulberry, and protein, and Example 8, which contained kale, gymnema, and sucralose, showed a particularly significant increase, suggesting that excellent diet effects can be expected.

[0048] [Test Example 2] (Dispersibility test) Each raw material was sieved (nominal size: 710). When combining two or more raw materials, the amount required to measure the degree of dispersion was measured according to the ratio in Table 2, and the mixture was mixed in a plastic bag by shaking by hand until uniform.

[0049] 10 g of the test substance was weighed out and the degree of dispersion was measured using a powder specific evaluation device "Powder Tester PT-X" manufactured by Hosokawa Micron Corp. The measurement of the degree of dispersion was carried out according to the manual of the device.

[0050] The degree of dispersion is measured by the following method. A sample of a specified weight (W) is dropped onto a tray from a specified height, and the weight (W1) of the sample on the tray is measured.Furthermore, the degree of dispersion is calculated using the following formula.

[0051] Dispersion degree (%)=(W-W1) / W×100

[0052] The composition of each test substance and the results are shown in Table 2 and Figures 1 to 5.

[0053] [Table 2]

[0054] As shown in Table 2, the compositions of the present invention in which other ingredients were blended with kale all had higher dispersibility and improved dispersibility than kale alone. Furthermore, the dispersibility was higher than the theoretical value of dispersibility expected when each ingredient was blended alone (value calculated based on the blending ratio using the values ​​from the comparative examples), and the dispersibility was improved.

[0055] As shown in Figure 1, the compositions of the present invention, in which kale and Kuma bamboo were further blended with any other material other than Kuma bamboo, showed significantly improved dispersibility in the three combinations of kale and Kuma bamboo further blended with Gymnema (Example 14) compared to the two combinations of kale and Kuma bamboo (Example 9).The three combinations of kale and Kuma bamboo further blended with one other material selected from mulberry leaves, protein, and sucralose (Examples 17, 18, and 19) showed lower dispersibility than the two combinations of kale and Kuma bamboo, but showed improved dispersibility compared to the ideal value.

[0056] As shown in Figure 2, the compositions of the present invention (Examples 15, 17, 20, and 21), which contain kale and mulberry leaves and any other material other than mulberry leaves, showed significantly improved dispersibility compared to the two combinations of kale and mulberry leaves (Example 10).

[0057] As shown in Figure 3, the compositions of the present invention, which are made by blending kale and protein with one other ingredient other than protein, showed significantly improved dispersibility in the three combinations (Examples 15, 18, and 23) in which kale and protein were further blended with one other ingredient selected from mulberry leaves, Kumazasa bamboo, and sucralose, compared to the two combinations (Example 11) in which kale and protein were blended. The three combinations (Example 22) in which kale, protein, and Gymnema were blended showed dispersibility similar to that of the two combinations (Example 11) in which kale and protein were blended, but showed improved dispersibility compared to the ideal value.

[0058] As shown in Figure 4, the compositions of the present invention, in which kale and gymnema were further blended with any other ingredient other than gymnema, and the three combinations of kale and gymnema further blended with kumazasa (Example 14) showed improved dispersibility compared to the two combinations of kale and gymnema (Example 12). The compositions of the present invention (Examples 16, 20, and 22), in which kale and gymnema were further blended with one other ingredient selected from sucralose, mulberry leaves, and protein, showed lower dispersibility than the two combinations of kale and gymnema (Example 12), but still showed improved dispersibility compared to the ideal value.

[0059] As shown in Figure 5, the compositions of the present invention (Examples 16, 19, 21, and 23), which contain kale and sucralose in addition to any other ingredient other than sucralose, showed significantly improved dispersibility compared to the two combinations of kale and sucralose (Example 13).

[0060] From the above, it can be seen that the composition of the present invention has a high dispersibility, and can be obtained simply by mixing kale with other ingredients, without requiring complicated operations such as processing raw materials or supplying air during mixing.

[0061] [Combination example] The powdered food and drink compositions (oral compositions of the present invention) shown in Table 3 were prepared. 3 g of the obtained composition of the present invention was mixed with 150 mL of water and drank. The odor and taste of kale were improved, and a beverage with excellent overall palatability was obtained.

[0062] [Table 3] [Industrial Applicability]

[0063] The oral composition of the present invention can be used as a so-called health food or the like, and is therefore industrially useful.

Claims

[Claim 1] An oral composition comprising kale and protein (excluding the following (1) to (13)). (1) A gamma-aminobutyric acid-containing food product containing a quantity of gamma-aminobutyric acid that provides an immediate effect of suppressing autonomic nervous activity related to elevated blood pressure. (2) An orally administered supplement characterized by containing seafood and mushrooms. (3) An oral composition comprising Peucedanum japonicum and at least one bacterium selected from the group consisting of Bifidobacterium and lactic acid bacteria. (4) A composition characterized by containing kale, propolis, or an extract thereof. (5) A health food characterized by containing processed kale, pine bark extract, brown sugar, and water-soluble dietary fiber. (6) An edible composition for green juice that uses powder of barley stems and / or leaves, characterized in that the barley is at least one variety of barley selected from Toyonokaze and Sachiho Golden. (7) A powdered green juice beverage characterized by containing (A) curcumin, (B) epigallocatechin gallate, (C) hesperidin, (D) quercetin, (E) isoflavone, (F) ferulic acid or a derivative thereof, (G) chlorogenic acid, (H) gallic acid, and (I) rutin. (8) An oral composition containing chocolate and peanuts. (9) Oral composition containing banana, orange, pineapple, and apple (10) An oral composition containing coenzyme Q10, ornithine, hyaluronic acid, polyphenols, and dietary fiber. (11) An oral composition containing kale, broccoli, pomegranate, and wolfberry. (12) An oral composition containing oat powder. (13) An oral composition containing soy protein, collagen, water-soluble dietary fiber, lactic acid bacteria, vitamins, and minerals.

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