Oligonucleotides for detecting human papillomavirus
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- TOYOBO CO LTD
- Filing Date
- 2024-11-28
- Publication Date
- 2026-06-09
Smart Images
Figure 2026093703000001 
Figure 2026093703000002 
Figure 2026093703000003
Abstract
Claims
1. A method for detecting human papillomavirus (HPV) types 26, 53, 67, 69, 70, 73, and / or 82 that may be present in a sample, the following: (a) A primer set for detecting HPV type 26 that can amplify the region containing the nucleotide sequences 349 to 371 in the nucleotide sequence shown in Sequence ID No. 15; (b) A primer set for detecting HPV type 53 that can amplify the region containing the nucleotide sequences 180 to 202 in the nucleotide sequence shown in Sequence ID No. 16; (c) A primer set for detecting HPV type 67 that can amplify the region containing the nucleotide sequences 586 to 605 in the nucleotide sequence shown in Sequence ID No. 17; (d) A primer set for detecting HPV type 69 that can amplify the region containing the nucleotide sequences 349 to 371 in the nucleotide sequence shown in Sequence ID No. 18; (e) A primer set for detecting HPV type 70 that can amplify the region containing the nucleotide sequences 40 to 59 in the nucleotide sequence shown in Sequence ID No. 19; (f) A primer set for detecting HPV type 73 that can amplify the region containing the nucleotide sequences 388 to 409 in the nucleotide sequence shown in Sequence ID No. 20; and (g) A nucleic acid primer set for detecting HPV82 that can amplify the region containing the nucleotide sequences 340 to 364 in the nucleotide sequence shown in Sequence ID No.
21. A method comprising the step of carrying out a nucleic acid amplification reaction using a reaction solution 1 containing at least one primer set selected from the group consisting of the following.
2. When the sample may contain HPV types 16, 18, 31, 33, 45, 52, 58, 35, 39, 51, 56, 59, 66, and / or 68, the reaction solution 1 is as follows: (h) A primer set for detecting HPV type 16 that can amplify the region containing the nucleotide sequences 401 to 421 in the nucleotide sequence shown in Sequence ID No. 1; (i) A primer set for detecting HPV type 18 that can amplify the region containing the nucleotide sequence 550 to 573 in the nucleotide sequence shown in Sequence ID No. 2; (j) A primer set for detecting HPV type 31 that can amplify the region containing the nucleotide sequence 532-553 in the nucleotide sequence shown in Sequence ID No. 3; (k) A primer set for detecting HPV type 33 that can amplify the region containing the nucleotide sequences 542 to 567 in the nucleotide sequence shown in Sequence ID No. 4; (l) A primer set for detecting HPV type 45 that can amplify the region containing the nucleotide sequences 418 to 438 in the nucleotide sequence shown in Sequence ID No. 7; (m) A primer set for detecting HPV type 52 that can amplify the region containing the nucleotide sequences 557 to 578 in the nucleotide sequence shown in Sequence ID No. 9; (n) A primer set for detecting HPV type 58 that can amplify the region containing the nucleotide sequences 542 to 567 in the nucleotide sequence shown in Sequence ID No. 11; (o) A primer set for detecting HPV type 35 that can amplify the region containing the nucleotide sequences 536 to 557 in the nucleotide sequence shown in Sequence ID No. 5; (p) A primer set for detecting HPV type 39 that can amplify the region containing the nucleotide sequences 751 to 776 in the nucleotide sequence shown in Sequence ID No. 6; (q) A primer set for detecting HPV type 51 that can amplify the region containing the nucleotide sequences 697 to 717 in the nucleotide sequence shown in Sequence ID No. 8; (r) A primer set for detecting HPV type 56 that can amplify the region containing the nucleotide sequences 751 to 775 in the nucleotide sequence shown in Sequence ID No. 10; (s) A primer set for detecting HPV type 59 that can amplify the region containing the nucleotide sequence 750 to 775 in the nucleotide sequence shown in Sequence ID No. 12; (t) A primer set for detecting HPV66 that can amplify the region containing the nucleotide sequences 751 to 775 in the nucleotide sequence shown in Sequence ID No. 13; and (u) A primer set for detecting HPV68 that can amplify the region containing the nucleotide sequences 752 to 777 in the nucleotide sequence shown in Sequence ID No.
14. The method according to claim 1, comprising at least one primer set selected from the group consisting of the following.
3. The primer set (a) is A primer having a consecutive nucleotide sequence including positions 296-322 in Sequence ID No. 15, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 375-395 in Sequence ID No. 15, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The primer set (b) is A primer having a consecutive nucleotide sequence including positions 147 to 171 in Sequence ID No. 16, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 244-267 in Sequence ID No. 16, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The primer set (c) is A primer having a consecutive nucleotide sequence including positions 544 to 564 in Sequence ID No. 17, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 647 to 667 in Sequence ID No. 17, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those sequences; The aforementioned primer set (d) A primer having a consecutive nucleotide sequence including positions 296-322 in Sequence ID No. 18, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 374-395 in Sequence ID No. 18, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those sequences; The primer set (e) is A primer having a consecutive nucleotide sequence including positions 17-36 in Sequence ID No. 19, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 169-188 in Sequence ID No. 19, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (f) A primer having a consecutive nucleotide sequence including positions 319-343 in Sequence ID No. 20, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 431-452 in Sequence ID No. 20, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; and / or The primer set (g) is A primer having a consecutive nucleotide sequence including positions 315-334 in Sequence ID No. 21, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 398 to 417 in Sequence ID No. 21, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The method according to claim 1, wherein in each primer set, one primer is complementary to the extension product of the other primer.
4. The aforementioned primer set (h) A primer having a consecutive nucleotide sequence including positions 285 to 307 in Sequence ID No. 1, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 422-443 in Sequence ID No. 1, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The primer set (i) A primer having a consecutive nucleotide sequence including positions 488 to 509 in Sequence ID No. 2, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 621-642 in Sequence ID No. 2, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The aforementioned primer set (j) A primer having a consecutive nucleotide sequence including positions 488 to 509 in Sequence ID No. 3, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a continuous nucleotide sequence including positions 581 to 600 in Sequence ID No. 3, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (k) A primer having a consecutive nucleotide sequence including positions 503 to 525 in Sequence ID No. 4, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 592-610 in Sequence ID No. 4, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (l) A primer having a consecutive nucleotide sequence including positions 366 to 388 in Sequence ID No. 7, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 452-473 in Sequence ID No. 7, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The aforementioned primer set (m) A primer having a consecutive nucleotide sequence including positions 454 to 476 in Sequence ID No. 9, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 583 to 602 in Sequence ID No. 9, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (n) A primer having a consecutive nucleotide sequence including positions 503 to 525 in Sequence ID No. 11, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 595 to 613 in Sequence ID No. 11, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those sequences; The primer set (o) A primer having a consecutive nucleotide sequence including positions 475-499 in Sequence ID No. 5, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 560-582 in Sequence ID No. 5, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The aforementioned primer set (p) A primer having a consecutive nucleotide sequence including positions 599 to 620 in Sequence ID No. 6, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 807 to 827 in Sequence ID No. 6, a complementary nucleotide sequence thereof, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (q) A primer having a consecutive nucleotide sequence including positions 656 to 677 in Sequence ID No. 8, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 741-762 in Sequence ID No. 8, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The aforementioned primer set (r) A primer having a consecutive nucleotide sequence including positions 727 to 747 in Sequence ID No. 10, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 789 to 808 in Sequence ID No. 10, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (s) A primer having a consecutive nucleotide sequence including positions 721-740 in Sequence ID No. 12, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 803 to 823 in Sequence ID No. 12, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; The aforementioned primer set (t) A primer having a consecutive nucleotide sequence including positions 727-747 in Sequence ID No. 13, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences, A primer set comprising primers having a consecutive nucleotide sequence including positions 789-808 in Sequence ID No. 13, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; and / or The aforementioned primer set (u) A primer having a consecutive nucleotide sequence including positions 597-660 in Sequence ID No. 14, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers having a continuous nucleotide sequence including positions 804-826 in Sequence ID No. 14, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; The method according to claim 2, wherein in each primer set, one primer is complementary to the extension product of the other primer.
5. The primer set (a) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 54, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 55, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (b) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 57, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 58, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (c) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 60, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 61, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (d) A primer consisting of the nucleotide sequence shown in Sequence ID No. 54, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 63, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (e) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 64, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 65, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (f) A primer consisting of the nucleotide sequence shown in Sequence ID No. 67, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 68, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; and / or The primer set (g) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 70, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 71, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The method according to claim 1, wherein in each primer set, one primer is complementary to the extension product of the other primer.
6. The aforementioned primer set (h) A primer consisting of the nucleotide sequence shown in Sequence ID No. 22, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 23, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (i) A primer consisting of the nucleotide sequence shown in Sequence ID No. 25, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 26, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (j) A primer consisting of the nucleotide sequence shown in Sequence ID No. 28, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 29, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (k) A primer consisting of the nucleotide sequence shown in Sequence ID No. 31, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 32, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (l) A primer consisting of the nucleotide sequence shown in Sequence ID No. 40, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 41, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (m) A primer consisting of the nucleotide sequence shown in Sequence ID No. 46, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 47, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (n) A primer consisting of the nucleotide sequence shown in Sequence ID No. 31, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 32, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (o) A primer consisting of the nucleotide sequence shown in Sequence ID No. 34, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 35, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (p) A primer consisting of the nucleotide sequence shown in Sequence ID No. 37, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 38, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (q) A primer consisting of the nucleotide sequence shown in Sequence ID No. 43, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 44, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The primer set (r) is A primer consisting of the nucleotide sequence shown in Sequence ID No. 49, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 50, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (s) A primer consisting of the nucleotide sequence shown in Sequence ID No. 52, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 38, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The aforementioned primer set (t) A primer consisting of the nucleotide sequence shown in Sequence ID No. 49, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 50, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; and / or The aforementioned primer set (u) A primer consisting of the nucleotide sequence shown in Sequence ID No. 37, its complementary nucleotide sequence, or a nucleotide sequence in which one to three nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set comprising primers consisting of the nucleotide sequence shown in Sequence ID No. 53, its complementary nucleotide sequence, or nucleotide sequences in which one to three nucleotides are substituted, deleted, inserted, or added; The method according to claim 2, wherein in each primer set, one primer is complementary to the extension product of the other primer.
7. The method according to claim 2, wherein the reaction solution 1 includes at least one primer set selected from the group consisting of primer sets (o) to (u).
8. The method according to claim 2, comprising the step of carrying out a nucleic acid amplification reaction using a reaction solution 2 containing at least one primer set selected from the group consisting of primer sets (h) to (n).
9. The reaction solution 1 is as follows: (A) HPV26 detection probes having a continuous nucleotide sequence including positions 349 to 371 in Sequence ID No. 15, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (B) HPV53 detection probes having a continuous nucleotide sequence including positions 180 to 202 in Sequence ID No. 16, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (C) HPV67 detection probes having a continuous nucleotide sequence including positions 586 to 605 in Sequence ID No. 17, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (D) HPV69 detection probes having a continuous nucleotide sequence including positions 349 to 371 in Sequence ID No. 18, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (E) HPV 70 detection probes having a continuous nucleotide sequence including positions 40-59 in Sequence ID No. 19, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (F) HPV73 detection probes having a continuous nucleotide sequence including positions 388 to 409 in Sequence ID No. 20, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; and (G) HPV82 detection probe having a continuous nucleotide sequence including positions 340 to 364 in Sequence ID No. 21, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences. The method according to claim 1, comprising the step of detecting the HPV type amplified by the nucleic acid amplification reaction of the reaction solution 1 using the probe, wherein the probe comprises at least one probe selected from the group consisting of the following:
10. The reaction solution 1 is as follows: (H) HPV16 detection probes having a continuous nucleotide sequence including positions 401 to 421 in Sequence ID No. 1, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (I) HPV18 detection probes having a continuous nucleotide sequence including positions 550 to 573 in Sequence ID No. 2, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (J) HPV31 detection probe having a consecutive nucleotide sequence including positions 532 to 553 in Sequence ID No. 3, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (K) HPV33 detection probe having a continuous nucleotide sequence including positions 542 to 567 in Sequence ID No. 4, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (L) HPV 45 detection probe having a continuous nucleotide sequence including positions 418 to 438 in Sequence ID No. 7, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (M) HPV52 detection probe having a continuous nucleotide sequence including positions 557 to 578 in Sequence ID No. 9, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (N) HPV58 detection probe having a continuous nucleotide sequence including positions 542 to 567 in Sequence ID No. 11, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (O) HPV35 detection probes having a continuous nucleotide sequence including positions 536 to 557 in Sequence ID No. 5, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (P) HPV39 detection probes having a continuous nucleotide sequence including positions 751 to 776 in Sequence ID No. 6, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (Q) HPV51 detection probe having a continuous nucleotide sequence including positions 697 to 717 in Sequence ID No. 8, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (R) HPV56 detection probe having a continuous nucleotide sequence including positions 751 to 775 in Sequence ID No. 10, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (S) A probe for detecting HPV type 59 having a continuous nucleotide sequence including positions 750 to 775 in sequence number 12, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences. (T) HPV66 detection probes having a continuous nucleotide sequence including positions 751 to 775 in Sequence ID No. 13, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; and (U) HPV68 detection probe having a consecutive nucleotide sequence including positions 752-777 in Sequence ID No. 14, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences. The method according to claim 9, comprising at least one probe selected from the group consisting of the following.
11. The probe (A) is a probe consisting of the base sequence shown in Sequence ID No. 56, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (B) is a probe consisting of the base sequence shown in Sequence ID No. 59, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (C) is a probe consisting of the base sequence shown in Sequence ID No. 62, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (D) is a probe consisting of the base sequence shown in Sequence ID No. 56, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (E) is a probe consisting of the base sequence shown in Sequence ID No. 66, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (F) is a probe consisting of the base sequence shown in Sequence ID No. 69, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; and / or The method according to claim 9, wherein the probe (G) is a probe comprising the base sequence shown in Sequence ID No. 72, a complementary base sequence thereof, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences.
12. The probe (H) is a probe consisting of the base sequence shown in Sequence ID No. 24, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (I) is a probe consisting of the base sequence shown in Sequence ID No. 27, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (J) is a probe consisting of the base sequence shown in Sequence ID No. 30, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (K) is a probe consisting of the base sequence shown in Sequence ID No. 33, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (L) is a probe consisting of the base sequence shown in Sequence ID No. 42, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (M) is a probe consisting of the base sequence shown in Sequence ID No. 48, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (N) is a probe consisting of the base sequence shown in Sequence ID No. 33, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (O) is a probe consisting of the base sequence shown in Sequence ID No. 36, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (P) is a probe consisting of the base sequence shown in Sequence ID No. 39, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (Q) is a probe consisting of the base sequence shown in Sequence ID No. 45, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (R) is a probe consisting of the base sequence shown in Sequence ID No. 51, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (S) is a probe consisting of the base sequence shown in Sequence ID No. 39, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The probe (T) is a probe consisting of the base sequence shown in Sequence ID No. 51, its complementary base sequence, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences; The method according to claim 10, wherein the probe (U) is a probe comprising the base sequence shown in Sequence ID No. 39, a complementary base sequence thereof, or a base sequence in which one to three bases are substituted, deleted, inserted, or added in those base sequences.
13. The method according to claim 10, wherein the reaction solution 1 includes at least one probe selected from the group consisting of probes (O) to (U).
14. The method according to claim 10, wherein the reaction solution 2 includes at least one probe selected from the group consisting of (H) to (N), and the method includes the step of detecting the HPV type amplified by the nucleic acid amplification reaction of the reaction solution 2 using the probe.
15. The method according to claim 9, wherein the probe is a fluorescently labeled probe.
16. The method according to claim 15, wherein the fluorescently labeled probe is a TaqMan probe.
17. The method according to claim 13, wherein probes (A) to (G) are labeled with a fluorescent compound detectable by the same fluorescent channel (CH1), probes (O) to (U) are labeled with a fluorescent compound detectable by the same fluorescent channel (CH2), and the fluorescent channels (CH1) and (CH2) are different from each other, making it possible to separately detect HPV types 26, 53, 67, 69, 70, 73, and / or 82, and HPV types 35, 39, 51, 56, 59, 66, and / or 68.
18. The method according to claim 14, wherein probe (H) is labeled with a fluorescent compound detectable by a fluorescent channel (CH3), probe (I) is labeled with a fluorescent compound detectable by a fluorescent channel (CH4), probes (J) to (N) are labeled with the same fluorescent channel (CH5), and the fluorescent channels (CH3), (CH4), and (CH5) are different from each other, making it possible to detect HPV type 16, HPV type 18, and HPV types 31, 33, 45, 52, and / or 58 separately.
19. The method according to claim 1, wherein the reaction solution 1 contains a heat-stable DNA polymerase belonging to Family A.
20. The method according to claim 8, wherein the reaction solution 2 contains a heat-stable DNA polymerase belonging to family A.
21. The method according to claim 20, wherein the heat-stable DNA polymerase belonging to Family A is at least one selected from the group consisting of Tth polymerase, Hawk Z05 polymerase, and their variants.
22. The method according to claim 1, wherein the reaction solution 1 contains 1 mM or more of divalent cations.
23. The method according to claim 8, wherein the reaction solution 2 contains 1 mM or more of divalent cations.
24. The method according to claim 1, which is a one-step RT-PCR method.
25. The method according to claim 1, wherein the sample is a sample that has not undergone nucleic acid purification.
26. The method according to claim 1, wherein the sample is a cervical swab sample, a mouthwash sample, a urine sample, or a cervical canal swab sample.
27. The method according to claim 1, wherein the sample is a suspension or concentrate thereof suspended in a liquid cytological preservation solution, water, physiological saline, or buffer solution.
28. The method according to claim 1, wherein the sample is a sample derived from an HPV-vaccinated subject.
29. The method according to claim 1, wherein the sample is a sample obtained by mixing the sample with an alkaline solution of 1 to 200 mM.
30. The method according to claim 29, wherein the alkaline solution is a sodium hydroxide solution, a potassium hydroxide solution, a calcium hydroxide solution, a lithium hydroxide aqueous solution, a barium hydroxide aqueous solution, or a strontium hydroxide aqueous solution.
31. A reagent or kit for use in the method according to any one of claims 1 to 30, comprising at least one primer set selected from the group consisting of primer sets (a) to (g), and possibly comprising at least one primer set selected from the group consisting of primer sets (h) to (n).
32. The reagent or kit according to claim 31, comprising at least one probe selected from the group consisting of probes (A) to (G), and possibly comprising at least one probe selected from the group consisting of probes (H) to (N).
33. At least one primer set selected from the following groups: (a) A primer having a consecutive nucleotide sequence including positions 296 to 322 in Sequence ID No. 15, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 26, comprising primers having a consecutive nucleotide sequence including positions 375 to 395 in Sequence ID No. 15, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (b) A primer having a consecutive nucleotide sequence including positions 147 to 171 in Sequence ID No. 16, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 53, comprising primers having a consecutive nucleotide sequence including positions 244-267 in Sequence ID No. 16, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (c) A primer having a consecutive nucleotide sequence including positions 544 to 564 in Sequence ID No. 17, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 67, comprising primers having a consecutive nucleotide sequence including positions 647 to 667 in Sequence ID No. 17, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (d) A primer having a consecutive nucleotide sequence including positions 296 to 322 in Sequence ID No. 18, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 69, comprising primers having a consecutive nucleotide sequence including positions 374-395 in Sequence ID No. 18, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (e) A primer having a consecutive nucleotide sequence including positions 17 to 36 in Sequence ID No. 19, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 70, comprising primers having a consecutive nucleotide sequence including positions 169 to 188 in Sequence ID No. 19, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (f) A primer having a consecutive nucleotide sequence including positions 319 to 343 in Sequence ID No. 20, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 73, comprising primers having a consecutive nucleotide sequence including positions 431 to 452 in Sequence ID No. 20, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (g) A primer having a consecutive nucleotide sequence including positions 315 to 334 in Sequence ID No. 21, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 82, comprising primers having a continuous nucleotide sequence including positions 398 to 417 in Sequence ID No. 21, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (h) A primer having a continuous nucleotide sequence including positions 285 to 307 in Sequence ID No. 1, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 16, comprising primers having a consecutive nucleotide sequence including positions 422-443 in Sequence ID No. 1, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (i) A primer having a consecutive nucleotide sequence including positions 488 to 509 in Sequence ID No. 2, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 18, comprising primers having a consecutive nucleotide sequence including positions 621-642 in Sequence ID No. 2, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (j) A primer having a consecutive nucleotide sequence including positions 488 to 509 in Sequence ID No. 3, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 31, comprising primers having a continuous nucleotide sequence including positions 581 to 600 in Sequence ID No. 3, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (k) A primer having a continuous nucleotide sequence including positions 503 to 525 in Sequence ID No. 4, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 33, comprising primers having a continuous nucleotide sequence including positions 592 to 610 in Sequence ID No. 4, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (l) A primer having a consecutive nucleotide sequence including positions 366 to 388 in Sequence ID No. 7, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 45, comprising primers having a consecutive nucleotide sequence including positions 452-473 in Sequence ID No. 7, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (m) A primer having a continuous nucleotide sequence including positions 454 to 476 in Sequence ID No. 9, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 52, comprising primers having a consecutive nucleotide sequence including positions 583 to 602 in Sequence ID No. 9, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (n) A primer having a continuous nucleotide sequence including positions 503 to 525 in Sequence ID No. 11, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 58, comprising primers having a consecutive nucleotide sequence including positions 595 to 613 in Sequence ID No. 11, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (o) A primer having a continuous nucleotide sequence including positions 475 to 499 in Sequence ID No. 5, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 35, comprising primers having a continuous nucleotide sequence including positions 560 to 582 in Sequence ID No. 5, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (p) A primer having a consecutive nucleotide sequence including positions 599 to 620 in Sequence ID No. 6, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 39, comprising primers having a consecutive nucleotide sequence including positions 807 to 827 in Sequence ID No. 6, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (q) A primer having a consecutive nucleotide sequence including positions 656 to 677 in Sequence ID No. 8, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 51, comprising primers having a consecutive nucleotide sequence including positions 741-762 in Sequence ID No. 8, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (r) A primer having a consecutive nucleotide sequence including positions 727 to 747 in Sequence ID No. 10, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 56, comprising primers having a consecutive nucleotide sequence including positions 789 to 808 in Sequence ID No. 10, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 (preferably 1 or 2, more preferably 1) nucleotides are substituted, deleted, inserted, or added; (s) A primer having a sequence of bases including positions 721 to 740 in Sequence ID No. 12, a complementary sequence thereof, or a sequence in which 1 to 3 bases (preferably 1 or 2, more preferably 1) are substituted, deleted, inserted, or added in those sequences, A primer set for detecting HPV type 59, comprising primers having a consecutive nucleotide sequence including positions 803 to 823 in Sequence ID No. 12, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (t) A primer having a consecutive nucleotide sequence including positions 727 to 747 in Sequence ID No. 13, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, A primer set for detecting HPV type 66, comprising primers having a continuous nucleotide sequence including positions 789 to 808 in Sequence ID No. 13, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; and (u) A primer having a continuous nucleotide sequence including positions 597 to 660 in Sequence ID No. 14, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences, HPV68 detection primer set comprising primers having a consecutive nucleotide sequence including positions 804-826 in Sequence ID No. 14, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added to those sequences. (Here, in each primer set, one primer is complementary to the extension product of the other primer.)
34. At least one probe selected from the following group: (A) HPV26 detection probes having a continuous nucleotide sequence including positions 349 to 371 in Sequence ID No. 15, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (B) HPV53 detection probes having a continuous nucleotide sequence including positions 180 to 202 in Sequence ID No. 16, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (C) HPV67 detection probes having a continuous nucleotide sequence including positions 586 to 605 in Sequence ID No. 17, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (D) HPV69 detection probes having a continuous nucleotide sequence including positions 349 to 371 in Sequence ID No. 18, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (E) HPV 70 detection probes having a continuous nucleotide sequence including positions 40-59 in Sequence ID No. 19, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1-3 nucleotides are substituted, deleted, inserted, or added in those sequences; (F) HPV 73 detection probes having a continuous nucleotide sequence including positions 388 to 409 in Sequence ID No. 20, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (G) HPV82 detection probes having a continuous nucleotide sequence including positions 340 to 364 in Sequence ID No. 21, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (H) HPV16 detection probes having a continuous nucleotide sequence including positions 401 to 421 in Sequence ID No. 1, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (I) HPV18 detection probes having a continuous nucleotide sequence including positions 550 to 573 in Sequence ID No. 2, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (J) HPV31 detection probe having a consecutive nucleotide sequence including positions 532 to 553 in Sequence ID No. 3, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (K) HPV33 detection probe having a continuous nucleotide sequence including positions 542 to 567 in Sequence ID No. 4, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (L) HPV 45 detection probe having a continuous nucleotide sequence including positions 418 to 438 in Sequence ID No. 7, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (M) HPV52 detection probe having a continuous nucleotide sequence including positions 557 to 578 in Sequence ID No. 9, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (N) HPV58 detection probe having a continuous nucleotide sequence including positions 542 to 567 in Sequence ID No. 11, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (O) HPV35 detection probes having a continuous nucleotide sequence including positions 536 to 557 in Sequence ID No. 5, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; (P) HPV39 detection probes having a continuous nucleotide sequence including positions 751 to 776 in Sequence ID No. 6, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (Q) HPV51 detection probe having a continuous nucleotide sequence including positions 697 to 717 in Sequence ID No. 8, a complementary nucleotide sequence thereto, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added; (R) HPV56 detection probe having a continuous nucleotide sequence including positions 751 to 775 in Sequence ID No. 10, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those sequences; (S) A probe for detecting HPV type 59 having a continuous nucleotide sequence including positions 750 to 775 in sequence number 12, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences. (T) HPV66 detection probes having a continuous nucleotide sequence including positions 751 to 775 in Sequence ID No. 13, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences; and (U) A probe for detecting HPV type 68 having a continuous nucleotide sequence including positions 752 to 777 in sequence number 14, a complementary nucleotide sequence thereof, or a nucleotide sequence in which 1 to 3 nucleotides are substituted, deleted, inserted, or added in those nucleotide sequences.
35. A method for detecting human papillomavirus (HPV) types 16, 18, 31, 35, 45, 52, 58, 35, 39, 51, 56, 59, 66, 68, 26, 53, 73, and / or 82 that may be present in a sample, A step of performing a nucleic acid amplification reaction using reaction solution 1 containing the first group of HPV type detection primer sets, and The process includes a step of performing a nucleic acid amplification reaction using reaction solution 2 containing a second group of HPV type detection primer sets, The first group of HPV types consists of HPV types 16, 18, 31, 33, 45, 52, and 58. A method wherein the second group of HPV types consists of HPV types 35, 39, 51, 56, 59, 66, 68, 26, 53, 73, and 82.
36. A method for detecting human papillomavirus (HPV) types 16, 18, 31, 35, 45, 52, 58, 35, 39, 51, 56, 59, 66, 68, 26, 53, 67, 69, 70, 73, and / or 82 that may be present in a sample, A step of performing a nucleic acid amplification reaction using reaction solution 1 containing the first group of HPV type detection primer sets, and The process includes a step of performing a nucleic acid amplification reaction using reaction solution 2 containing a second group of HPV type detection primer sets, The first group of HPV types consists of HPV types 16, 18, 31, 33, 45, 52, and 58. A method wherein the second group of HPV types consists of HPV types 35, 39, 51, 56, 59, 66, 68, 26, 53, 67, 69, 70, 73, and 82.