Method for measuring human neutrophil elastase
The method employs antibodies recognizing distinct epitopes on the neutrophil elastase-A1AT complex to overcome sensitivity issues in conventional assays, achieving effective detection in the presence of high A1AT levels.
JP2026100970APending Publication Date: 2026-06-22SUMITOMO BAKELITE CO LTD
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- SUMITOMO BAKELITE CO LTD
- Filing Date
- 2024-12-10
- Publication Date
- 2026-06-22
AI Technical Summary
Technical Problem
Conventional immunoassays for neutrophil elastase suffer from reduced sensitivity due to the high presence of α1-antitrypsin (A1AT) in biological samples, which binds to anti-A1AT antibodies, hindering effective detection.
Method used
A method using two or more antibodies that recognize different epitopes on the complex of neutrophil elastase and its inhibitor, such as α1-antitrypsin, allowing for high-sensitivity detection without the need for additional inhibitor addition.
Benefits of technology
Enables sensitive detection of neutrophil elastase in samples with high A1AT levels by utilizing antibodies that specifically bind to the complex, enhancing assay performance.
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Smart Images
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Abstract
The objective was to develop a novel neutrophil elastase measurement method that can measure neutrophil elastase with high sensitivity. [Solution] A method for measuring human neutrophil elastase in a sample is provided, comprising contacting the sample with two or more antibodies or antigen-binding fragments thereof that recognize different epitopes on a complex with human neutrophil elastase. A reagent or kit for immunological measurement of human neutrophil elastase is also provided, comprising two or more antibodies or antigen-binding fragments thereof that recognize different epitopes on a complex with human neutrophil elastase and its inhibitor.
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