Use of nitrogen-doped carbon dot nanomaterials in CRISPR / Cas preparation and soil tolerance mitigation
Nitrogen-doped carbon dots enhance CRISPR/Cas9 delivery for effective ARG knockout in soil by forming a stable and environmentally friendly composite, addressing inefficiencies in existing systems.
JP2026519412APending Publication Date: 2026-06-16JIANGNAN UNIV
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2023-10-19
- Publication Date
- 2026-06-16
AI Technical Summary
Technical Problem
Current technologies lack effective delivery systems for CRISPR/Cas9 to target and knockout antibiotic resistance genes (ARGs) in soil, and existing nanomaterials are not stable, environmentally friendly, or efficient.
Method used
Utilizing nitrogen-doped carbon dots (NCDs) as a delivery carrier to prepare an NCDs-Cas9/sgRNA composite material for targeted knockout of ARGs in soil.
Benefits of technology
The NCDs-Cas9/sgRNA nanocomposite achieves efficient and targeted removal of multiple ARGs in soil, with improved gene knockout efficiency and reduced environmental impact.
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Abstract
This invention discloses the use of nitrogen-doped carbon dot nanomaterials in the preparation of CRISPR / Cas and the mitigation of soil resistance, and belongs to the field of environmental functional materials. This invention selects NCDs as nanocarriers, designs specific sgRNAs using commonly found resistance genes in the environment as target ARGs, covalently bonds the NCDs via amide bonds to support Cas9 nuclease, and synthesizes nanocomposite materials. The coupling between NCDs and Cas9 was characterized using methods such as SDS-PAGE and transmission electron microscopy. In vitro tests demonstrated that the nanocomposite materials possess good nucleic acid endonuclease activity and targeting capabilities. The nanocomposite materials according to this invention can be widely applied to the removal of multiple antibiotic resistance genes, and targeted removal of antibiotic resistance genes can be achieved simply by substituting sgRNAs for different antibiotic resistance genes in the nanocomposite material.
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