Modified fibroin fibers

Abstract

The present invention provides a modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning, the modified fibroin fiber containing modified fibroin, and the fiber diameter of the raw fiber before being irreversibly shrunk is greater than 25 μm.

Description

[Technical field]

[0001] The present invention relates to modified fibroin fibers. [Background technology]

[0002] Fibroin is a type of fibrous protein that contains up to 90% glycine, alanine and serine residues that lead to the formation of β-pleated sheets (Non-Patent Document 1). Known examples of fibroin include proteins that constitute the silk produced by insects and spiders (silk proteins, hornet silk proteins, spider silk proteins, etc.).

[0003] Fibroin fibers obtained by spinning fibroin have the property of shrinking when they come into contact with moisture (for example, when immersed in water or hot water, or when exposed to a high humidity environment, etc.). This property causes various problems in the manufacturing process and commercialization, and also affects products made using the fibroin fibers.

[0004] As examples of shrink-proofing methods for preventing shrinkage of products, there have been reported a shrink-proofing processing method for silk fabrics (Patent Document 1), which is characterized by immersing, under tension, silk fabrics made from highly twisted yarn that have been scoured, in water, other solvents, or a mixture thereof and heating for a predetermined period of time, and a shape fixation method for animal fiber products (Patent Document 2), which is characterized by contacting an animal fiber product that has been molded into a required shape with high-pressure saturated steam at 120 to 200°C, thereby fixing the fiber product in the shape it had at the time of the steam treatment. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Special Publication No. 2-6869 [Patent Document 2] Japanese Patent Application Publication No. 6-294068 [Non-patent literature]

[0006] [Non-Patent Document 1] Asakura et al., Encyclopedia of Agricultural Science, Academic Press: New York, NY, 1994, Vol. 4, pp. 1-11 Summary of the Invention [Problem to be solved by the invention]

[0007] The shrink-proofing methods disclosed in Patent Documents 1 and 2 are for textile products, and are difficult to apply directly to shrink-proofing of raw fibers. These methods are not versatile for various products made using fibroin fibers. If it were possible to reduce the shrinkage of the fibroin fiber itself without using such shrink-proofing methods, it would be extremely useful industrially and versatile.

[0008] An object of the present invention is to provide a fibroin fiber in which shrinkage of the fiber itself is reduced. [Means for solving the problem]

[0009] The present inventors have conducted extensive research to solve the above problems. As a result, they have found that the shrinkage of modified fibroin fibers due to contact with water can be reduced by adjusting the fiber diameter of the modified fibroin fibers or the raw fiber from which the modified fibroin fibers are derived. The present invention is based on this novel finding.

[0010] That is, the present invention relates to, for example, the following inventions. [1] A modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning, the modified fibroin fiber containing modified fibroin, the fiber diameter of the raw fiber before being irreversibly shrunk being greater than 25 μm. [2] The modified fibroin fiber according to [1], wherein the shrinkage history is a shrinkage history in which the raw fiber is irreversibly shrunk by contacting it with water or a shrinkage history in which the raw fiber is irreversibly shrunk by heating and relaxing it. [3] The modified fibroin fiber according to [1] or [2], which is substantially free of residual stress caused by stretching during the spinning process. [4] The modified fibroin fiber according to any one of [1] to [3], which has a shrinkage percentage defined by the following formula (1) of 3.3% or less. Equation (1): Shrinkage rate (%) = (1 - (length of modified fibroin fiber when dried from a wet state / length of modified fibroin fiber when wet)) x 100 [5] The modified fibroin fiber according to any one of [1] to [4], wherein the modified fibroin is a modified spider silk fibroin. [6] The modified fibroin fiber according to any one of [1] to [5], wherein the modified fibroin is a hydrophobic modified spider silk fibroin. [7] The modified fibroin fiber according to any one of [1] to [6], which has a fiber diameter that is within ±20% of the fiber diameter of the raw fiber before the irreversible shrinkage. [8] The modified fibroin fiber according to any one of [1] to [7], wherein the cross-sectional shape is circular or elliptical. [9] The modified fibroin fiber according to any one of [1] to [8], having a matte appearance.

[10] A product comprising the modified fibroin fiber according to any one of [1] to [9].

[11] The product according to

[10] , wherein the product is selected from the group consisting of fibers, yarns, fabrics, knits, braids, nonwoven fabrics, paper, and cotton.

[12] A shrinking step is provided for irreversibly shrinking the raw fiber. The raw fiber contains modified fibroin, A method for producing a modified fibroin fiber, wherein the raw fiber has a fiber diameter of greater than 25 μm before the shrinking step.

[13] The manufacturing method described in

[12] , wherein in the shrinkage step, the raw fiber is irreversibly shrunk by contacting the raw fiber with water or by heating and relaxing the raw fiber.

[14] The manufacturing method according to

[12] or

[13] , wherein in the shrinking step, substantially all of the residual stress in the raw fiber caused by drawing in the spinning process is released.

[15] The method according to any one of

[12] to

[14] , wherein the modified fibroin is modified spider silk fibroin.

[16] The method according to any one of

[12] to

[15] , wherein the modified fibroin is a hydrophobic modified spider silk fibroin.

[17] A modified fibroin fiber comprising a modified fibroin, having a fiber diameter of more than 25 μm, and having a shrinkage rate defined by the following formula (1) of 3.3% or less. Equation (1): Shrinkage rate (%) = (1 - (length of modified fibroin fiber when dried from a wet state / length of modified fibroin fiber when wet)) x 100

[18] The modified fibroin fiber according to

[17] , wherein the modified fibroin fiber has a shrinkage history of being irreversibly shrunk after spinning.

[19] The modified fibroin fiber according to

[18] , having a fiber diameter of less than ±20% of the fiber diameter of the raw fiber before it is irreversibly shrunk.

[20] The modified fibroin fiber according to

[18] or

[19] , wherein the shrinkage history is a shrinkage history in which the raw fiber is irreversibly shrunk by contacting it with water or a shrinkage history in which the raw fiber is irreversibly shrunk by heating and relaxing it. [twenty one] The modified fibroin fiber according to any one of

[17] to

[20] , which is substantially free of residual stress caused by stretching during the spinning process. [twenty two] The modified fibroin fiber according to any one of

[17] to

[21] , wherein the modified fibroin is a modified spider silk fibroin. [twenty three] The modified fibroin fiber according to any one of

[17] to

[22] , wherein the modified fibroin is a hydrophobic modified spider silk fibroin. [twenty four] The modified fibroin fiber according to any one of

[17] to

[23] , wherein the cross-sectional shape is circular or elliptical. [twenty five] The modified fibroin fiber according to any one of

[17] to

[24] , having a matte appearance.

[26] A product comprising the modified fibroin fiber according to any one of

[17] to

[25] .

[27] The product according to

[26] , wherein the product is selected from the group consisting of fibers, yarns, fabrics, knits, braids, nonwoven fabrics, paper, and cotton. Effect of the Invention

[0011] According to the present invention, it is possible to provide a fibroin fiber in which the shrinkage of the fiber itself is reduced. [Brief description of the drawings]

[0012] [Figure 1] FIG. 2 is a schematic diagram showing an example of a domain sequence of a modified fibroin. [Diagram 2] FIG. 2 is a schematic diagram showing an example of a domain sequence of a modified fibroin. [Diagram 3] FIG. 2 is a schematic diagram showing an example of a domain sequence of a modified fibroin. [Figure 4] FIG. 1 is an explanatory diagram illustrating an example of a spinning apparatus for producing raw fibers. [Diagram 5] FIG. 1 is a diagram showing an example of change in length of raw fiber due to contact with water. [Figure 6] FIG. 1 is an explanatory diagram illustrating an example of a manufacturing apparatus for producing modified fibroin fibers. [Figure 7] FIG. 1 is an explanatory diagram illustrating an example of a manufacturing apparatus for producing modified fibroin fibers. [Figure 8]FIG. 1 is an explanatory diagram illustrating an example of a manufacturing apparatus for producing modified fibroin fibers. [Figure 9] 9 is an explanatory diagram showing a speed adjusting means and a temperature adjusting means which can be provided in the high-temperature heating furnace in FIG. 8. FIG. [Figure 10] 1 is a scanning electron microscope (SEM) image of the cross-sectional profile of a modified fibroin fiber according to one embodiment. [Figure 11] 1 is a graph showing an example of the results of a moisture absorption heat generation test. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] Hereinafter, embodiments of the present invention will be described in detail, however, the present invention is not limited to the following embodiments.

[0014] [Modified fibroin] The modified fibroin according to this embodiment is represented by the formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n The modified fibroin is a protein containing a domain sequence represented by a motif. The modified fibroin may have further amino acid sequences (N-terminal sequence and C-terminal sequence) added to either or both of the N-terminal and C-terminal sides of the domain sequence. The N-terminal sequence and the C-terminal sequence are typically, but are not limited to, regions that do not have repetitions of amino acid motifs characteristic of fibroin and consist of about 100 amino acid residues. In this embodiment, modified spider silk fibroin is preferably used as the modified fibroin because it is also excellent in heat retention, moisture absorption and heat generation properties, and / or flame retardancy.

[0015] As used herein, the term "modified fibroin" refers to artificially produced fibroin (artificial fibroin). The modified fibroin may be a fibroin whose domain sequence is different from the amino acid sequence of naturally occurring fibroin, or may be a fibroin whose domain sequence is identical to the amino acid sequence of naturally occurring fibroin. The "naturally occurring fibroin" referred to in this specification is also a fibroin represented by the formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n A protein containing a domain sequence represented by a motif.

[0016] A "modified fibroin" may be one that uses the amino acid sequence of naturally occurring fibroin as is, or one that has had its amino acid sequence modified based on the amino acid sequence of naturally occurring fibroin (for example, one that has had its amino acid sequence modified by modifying the gene sequence of a cloned naturally occurring fibroin), or one that has been artificially designed and synthesized independent of naturally occurring fibroin (for example, one having a desired amino acid sequence obtained by chemically synthesizing a nucleic acid that codes for a designed amino acid sequence).

[0017] As used herein, the term "domain sequence" refers to a crystalline region specific to fibroin (typically, the (A) n A motif) and an amorphous region (typically corresponding to REP of an amino acid sequence), and the amino acid sequence is represented by the formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n The amino acid sequence represented by the motif (A) n The motif shows an amino acid sequence mainly composed of alanine residues, and the number of amino acid residues is 2 to 27. (A) nThe number of amino acid residues in the motif may be an integer of 2 to 20, 4 to 27, 4 to 20, 8 to 20, 10 to 20, 4 to 16, 8 to 16, or 10 to 16. n The ratio of the number of alanine residues to the total number of amino acid residues in the motif may be 40% or more, and may be 60% or more, 70% or more, 80% or more, 83% or more, 85% or more, 86% or more, 90% or more, 95% or more, or 100% (meaning that the motif is composed of only alanine residues). n At least seven of the motifs may be composed of only alanine residues. REP represents an amino acid sequence composed of 2 to 200 amino acid residues. REP may be an amino acid sequence composed of 10 to 200 amino acid residues, or may be an amino acid sequence composed of 10 to 40, 10 to 60, 10 to 80, 10 to 100, 10 to 120, 10 to 140, 10 to 160, or 10 to 180 amino acid residues. m represents an integer of 2 to 300, and may be an integer of 8 to 300, 10 to 300, 20 to 300, 40 to 300, 60 to 300, 80 to 300, 10 to 200, 20 to 200, 20 to 180, 20 to 160, 20 to 140, or 20 to 120. (A) n The motifs may be identical to each other or different from each other in amino acid sequence, and the multiple REPs may be identical to each other or different from each other in amino acid sequence.

[0018] The modified fibroin according to the present embodiment can be obtained, for example, by modifying the amino acid sequence of the cloned gene sequence of naturally occurring fibroin by, for example, substituting, deleting, inserting, and / or adding one or more amino acid residues. Substitution, deletion, insertion, and / or addition of amino acid residues can be performed by methods well known to those skilled in the art, such as site-specific mutagenesis. Specifically, it can be performed according to the methods described in literature, such as Nucleic Acid Res. 10, 6487 (1982) and Methods in Enzymology, 100, 448 (1983).

[0019] Naturally occurring fibroin has the formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n A protein containing a domain sequence represented by a motif, specifically, for example, fibroin produced by insects or arachnids.

[0020] Examples of fibroin produced by insects include silk proteins produced by silkworms such as Bombyx mori, Bombyx mandarina, Antheraea yamamai, Antheraea pernyi, Eriogyna pyretorum, Pilosamia Cynthia ricini, Samia cynthia, Caligura japonica, Antheraea mylitta, and Antheraea assama, as well as hornet silk proteins excreted by the larvae of the Japanese hornet (Vespa simillima xanthoptera).

[0021] A more specific example of fibroin produced by an insect is, for example, silkworm fibroin L chain (GenBank accession numbers M76430 (nucleotide sequence) and AAA27840.1 (amino acid sequence)).

[0022] Examples of fibroin produced by spiders include spiders belonging to the Araneus genus, such as the Japanese squirrel spider, the Japanese garden squirrel spider, the red squirrel spider, the green squirrel spider, and the Japanese bean squirrel spider; spiders belonging to the Neoscona genus, such as the Japanese mountain squirrel spider, the Japanese house squirrel spider, and the Japanese Satsuma squirrel spider; spiders belonging to the Pronus genus, such as the Japanese squirrel spider; spiders belonging to the Cyrtarachne genus, such as the Japanese squirrel spider and the Japanese squirrel spider; spiders of the genus Gasteracantha such as the genus Acanthurus and the genus Siberian siberiana; spiders of the genus Ordgarius such as the genus Orbgarius and the genus Argiope such as the orb-weaver spider, the orb-weaver spider and the genus Arachnura such as the orb-weaver spider; spiders of the genus Acusilas such as the scraping spider; spiders of the genus Centipede such as the orb-weaver spider, the orb-weaver spider and the genus Centipede; spider silk proteins produced by spiders belonging to the genus Poltys such as the Japanese bush spider, the Japanese bush spider, the Japanese bush spider and the Japanese black bush spider, and spiders belonging to the genus Cyclosa such as the Japanese bush spider, the Japanese bush spider, the Japanese black bush spider and the Japanese black bush spider, and spiders belonging to the genus Chorizopes such as the Japanese black bush spider, and spiders belonging to the genus Tetragnatha such as the Japanese long-legged spider, the Japanese long-legged spider, the Japanese bush spider and the Japanese long-legged spider, and spiders belonging to the genus Tetragnatha such as the Japanese long-legged spider, the Japanese long-legged spider, the Japanese black bush spider and the Japanese black bush spider, spiders belonging to the genus Leucauge such as the medium-sized orb-weaver spider and the small orb-weaver spider; spiders belonging to the genus Nephila such as the orb-weaver spider and the giant orb-weaver spider; spiders belonging to the genus Menosira such as the golden spider; spiders belonging to the genus Dyschiriognatha such as the long-legged spider; spiders belonging to the genus Latrodectus such as the black widow spider, the redback spider, the gray widow spider and the three-spotted widow spider;Spider silk proteins include spider silk proteins produced by spiders belonging to the family Tetragnathidae, such as spiders belonging to the genus Euprosthenops. Examples of spider silk proteins include dragline proteins such as MaSp (MaSp1 and MaSp2) and ADF (ADF3 and ADF4), MiSp (MiSp1 and MiSp2), etc.

[0023] More specific examples of spider silk proteins produced by spiders include fibroin-3 (adf-3) [derived from Araneus diadematus] (GenBank accession numbers AAC47010 (amino acid sequence), U47855 (nucleotide sequence)), fibroin-4 (adf-4) [derived from Araneus diadematus] (GenBank accession numbers AAC47011 (amino acid sequence), U47856 (nucleotide sequence)), dragline silk protein spidroin 1 [derived from Nephila clavipes] (GenBank accession numbers AAC04504 (amino acid sequence), U37520 (nucleotide sequence)), major ampullate spidroin 1 [derived from Latrodectus hesperus] (GenBank accession numbers ABR68856 (amino acid sequence), EF595246 (nucleotide sequence)), dragline silk protein spidroin 2 [derived from Nephila clavata] (GenBank accession numbers AAL32472 (amino acid sequence), AF441245 (nucleotide sequence)), major ampullate spidroin 1 [Euprosthenops australis] (GenBank accession numbers CAJ00428 (amino acid sequence), AJ973155 (nucleotide sequence)), and major ampullate spidroin 2 [Euprosthenops australis] (GenBank accession numbers CAM32249.1 (amino acid sequence), AM490169 (nucleotide sequence)), minor ampullate silk protein 1 [Nephila clavipes] (GenBank accession number AAC14589.1 (amino acid sequence)), minor ampullate silk protein 2 [Nephila clavipes] (GenBank accession number AAC14591.1 (amino acid sequence)), minor ampullate spidroin-like protein [Nephilengys cruentata] (GenBank accession number ABR37278.1 (amino acid sequence) and the like.

[0024] More specific examples of naturally derived fibroins include fibroins whose sequence information is registered in NCBI GenBank. For example, from among the sequence information registered in NCBI GenBank, sequences that include INV as DIVISION can be confirmed by extracting sequences with spidroin, ampullate, fibroin, "silk and polypeptide," or "silk and protein" as keywords in DEFINITION, sequences with a specific product character string in CDS, and sequences with a specific character string in TISSUE TYPE in SOURCE.

[0025] The modified fibroin according to the present embodiment may be modified silk fibroin (a silk protein produced by a silkworm with a modified amino acid sequence) or modified spider silk fibroin (a spider silk protein produced by an arachnid with a modified amino acid sequence). The modified fibroin is preferably modified spider silk fibroin.

[0026] Specific examples of modified fibroins include a modified fibroin derived from the major ampullate gland protein of spiders (first modified fibroin), a modified fibroin having a domain sequence with a reduced content of glycine residues (second modified fibroin), (A) n A modified fibroin having a domain sequence with a reduced content of motifs (third modified fibroin), the content of glycine residues, and (A) n These include a modified fibroin with a reduced motif content (the fourth modified fibroin), a modified fibroin with a domain sequence that includes a region of high local hydrophobicity index (the fifth modified fibroin), and a modified fibroin with a domain sequence with a reduced content of glutamine residues (the sixth modified fibroin).

[0027] The first modified fibroin is represented by the formula 1: [(A) nMotif-REP] m In the first modified fibroin, a protein having a domain sequence represented by the formula: n The number of amino acid residues in the motif is preferably an integer of 3 to 20, more preferably an integer of 4 to 20, even more preferably an integer of 8 to 20, even more preferably an integer of 10 to 20, even more preferably an integer of 4 to 16, particularly preferably an integer of 8 to 16, and most preferably an integer of 10 to 16. The first modified fibroin has a number of amino acid residues constituting REP in formula 1 of preferably 10 to 200 residues, more preferably 10 to 150 residues, even more preferably 20 to 100 residues, and even more preferably 20 to 75 residues. The first modified fibroin has a number of amino acid residues constituting REP in formula 1: [(A) n Motif-REP] m The total number of glycine residues, serine residues and alanine residues contained in the amino acid sequence represented by the formula (I) is preferably 40% or more, more preferably 60% or more, and even more preferably 70% or more, based on the total number of amino acid residues.

[0028] The first modified fibroin has the formula 1: [(A) n Motif-REP] m and the C-terminal sequence is an amino acid sequence shown in any one of SEQ ID NOs: 1 to 3 or an amino acid sequence having 90% or more homology to the amino acid sequence shown in any one of SEQ ID NOs: 1 to 3.

[0029] The amino acid sequence shown in SEQ ID NO:1 is identical to the amino acid sequence consisting of 50 amino acid residues at the C-terminus of the amino acid sequence of ADF3 (GI:1263287, NCBI), the amino acid sequence shown in SEQ ID NO:2 is identical to the amino acid sequence obtained by removing 20 residues from the C-terminus of the amino acid sequence shown in SEQ ID NO:1, and the amino acid sequence shown in SEQ ID NO:3 is identical to the amino acid sequence obtained by removing 29 residues from the C-terminus of the amino acid sequence shown in SEQ ID NO:1.

[0030] A more specific example of the first modified fibroin is (1-i) a modified fibroin comprising an amino acid sequence shown in SEQ ID NO: 4 (recombinant spider silk protein ADF3KaiLargeNRSH1), or (1-ii) an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 4. The sequence identity is preferably 95% or more.

[0031] The amino acid sequence shown in SEQ ID NO:4 is an amino acid sequence of ADF3 with an amino acid sequence (SEQ ID NO:5) consisting of an initiation codon, a His10 tag, and an HRV3C protease (Human rhinovirus 3C protease) recognition site added to the N-terminus, in which the repeat region at positions 1 to 13 is increased approximately twice and mutated so that translation terminates at amino acid residue 1154. The amino acid sequence at the C-terminus of the amino acid sequence shown in SEQ ID NO:4 is identical to the amino acid sequence shown in SEQ ID NO:3.

[0032] The modified fibroin of (1-i) may consist of the amino acid sequence shown in SEQ ID NO:4.

[0033] The second modified fibroin has an amino acid sequence in which the domain sequence has a reduced content of glycine residues compared to naturally-derived fibroin. It can be said that the second modified fibroin has an amino acid sequence in which at least one or more glycine residues in REP have been replaced with another amino acid residue compared to naturally-derived fibroin.

[0034] The second modified fibroin may have an amino acid sequence in which, compared to a naturally occurring fibroin, at least one glycine residue in at least one motif sequence selected from GGX and GPGXX (wherein G represents a glycine residue, P represents a proline residue, and X represents an amino acid residue other than glycine) in REP is replaced with another amino acid residue in at least one or more of the motif sequences.

[0035] In the second modified fibroin, the proportion of the motif sequence in which the above-mentioned glycine residue is replaced with another amino acid residue may be 10% or more relative to the total motif sequence.

[0036] The second modified fibroin has the formula 1: [(A) n Motif-REP] m (A) is located at the most C-terminal side of the domain sequence. n The total number of amino acid residues of the amino acid sequence consisting of XGX (where X represents an amino acid residue other than glycine) contained in all REPs in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence is z, and the amino acid sequence located at the most C-terminus side from the domain sequence is (A). n From the motif above When the total number of amino acid residues in the sequence excluding the sequence up to the C-terminus of the amino acid sequence is w, z / w may be 30% or more, 40% or more, 50% or more, or 50.9% or more. (A) n The number of alanine residues relative to the total number of amino acid residues in the motif may be 83% or more, but is preferably 86% or more, more preferably 90% or more, even more preferably 95% or more, and even more preferably 100% (meaning that it is composed only of alanine residues).

[0037] The second modified fibroin is preferably one in which one glycine residue of the GGX motif is replaced with another amino acid residue to increase the content of the amino acid sequence consisting of XGX. The second modified fibroin preferably has a content of the amino acid sequence consisting of GGX in the domain sequence of 30% or less, more preferably 20% or less, even more preferably 10% or less, even more preferably 6% or less, even more preferably 4% or less, and particularly preferably 2% or less. The content of the amino acid sequence consisting of GGX in the domain sequence can be calculated by the same method as the calculation method for the content (z / w) of the amino acid sequence consisting of XGX below.

[0038] The method for calculating z / w will be described in more detail. First, the formula 1: [(A) n Motif-REP] m In a fibroin (modified fibroin or naturally-occurring fibroin) containing a domain sequence represented by the formula: n An amino acid sequence consisting of XGX is extracted from all REPs contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence. The total number of amino acid residues that make up XGX is z. For example, if 50 amino acid sequences consisting of XGX are extracted (no overlaps), z is 50 x 3 = 150. In addition, for example, in the case of an amino acid sequence consisting of XGXGX, if there are Xs contained in two XGXs (the central X), the overlaps are subtracted from the calculation (in the case of XGXGX, it is 5 amino acid residues). w is the number of amino acid residues (A) located at the most C-terminus side from the domain sequence. n It is the total number of amino acid residues contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence. For example, in the case of the domain sequence shown in Figure 1, w is 4 + 50 + 4 + 100 + 4 + 10 + 4 + 20 + 4 + 30 = 230 ((A) located at the most C-terminus side). n (The motif is excluded.) Next, z / w (%) can be calculated by dividing z by w.

[0039] Here, the z / w ratio in naturally derived fibroin will be described. First, as described above, fibroins whose amino acid sequence information is registered in NCBI GenBank were confirmed by the method exemplified above, and 663 types of fibroin (of which, 415 types were spider-derived fibroin) were extracted. Of all the extracted fibroins, those with the formula 1: [(A) n Motif-REP] m The z / w ratio was calculated by the above-mentioned calculation method from the amino acid sequence of naturally occurring fibroin, which contains a domain sequence represented by the formula (I) and the content of the amino acid sequence consisting of GGX in the fibroin is 6% or less. As a result, the z / w ratio of the naturally occurring fibroin was all less than 50.9% (the highest was 50.86%).

[0040] In the second modified fibroin, z / w is preferably 50.9% or more, more preferably 56.1% or more, even more preferably 58.7% or more, even more preferably 70% or more, and even more preferably 80% or more. There is no particular upper limit to z / w, but it may be, for example, 95% or less.

[0041] The second modified fibroin can be obtained, for example, by modifying the gene sequence of a cloned naturally-derived fibroin by substituting at least a part of the base sequence encoding a glycine residue to encode another amino acid residue. In this case, one glycine residue in the GGX motif and the GPGXX motif may be selected as the glycine residue to be modified, or may be substituted so that z / w is 50.9% or more. Alternatively, for example, an amino acid sequence satisfying the above-mentioned aspects may be designed from the amino acid sequence of a naturally-derived fibroin, and a nucleic acid encoding the designed amino acid sequence may be chemically synthesized to obtain the second modified fibroin. In either case, in addition to the modification equivalent to substituting the glycine residue in REP with another amino acid residue from the amino acid sequence of a naturally-derived fibroin, the amino acid sequence may be modified by substituting, deleting, inserting and / or adding one or more amino acid residues.

[0042] The other amino acid residue is not particularly limited as long as it is an amino acid residue other than a glycine residue, but is preferably a hydrophobic amino acid residue such as a valine (V) residue, a leucine (L) residue, an isoleucine (I) residue, a methionine (M) residue, a proline (P) residue, a phenylalanine (F) residue, or a tryptophan (W) residue, or a hydrophilic amino acid residue such as a glutamine (Q) residue, an asparagine (N) residue, a serine (S) residue, a lysine (K) residue, or a glutamic acid (E) residue, more preferably a valine (V) residue, a leucine (L) residue, an isoleucine (I) residue, a phenylalanine (F) residue, or a glutamine (Q) residue, and even more preferably a glutamine (Q) residue.

[0043] More specific examples of the second modified fibroin include (2-i) a modified fibroin comprising an amino acid sequence shown in SEQ ID NO: 6 (Met-PRT380), SEQ ID NO: 7 (Met-PRT410), SEQ ID NO: 8 (Met-PRT525) or SEQ ID NO: 9 (Met-PRT799), or (2-ii) an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9.

[0044] The modified fibroin (2-i) will be described. The amino acid sequence shown in SEQ ID NO:6 is obtained by replacing all GGX in the REP of the amino acid sequence shown in SEQ ID NO:10 (Met-PRT313) corresponding to naturally-occurring fibroin with GQX. The amino acid sequence shown in SEQ ID NO:7 is obtained by replacing every third amino acid (A) from the N-terminus to the C-terminus of the amino acid sequence shown in SEQ ID NO:6. n The motif was deleted, and the C-terminal sequence was further amplified with [(A) n The amino acid sequence shown in SEQ ID NO:8 is a sequence in which each of (A) of the amino acid sequence shown in SEQ ID NO:7 is inserted. n Two alanine residues were inserted at the C-terminus of the motif, and some glutamine (Q) residues were further replaced with serine (S) residues, with some amino acids deleted at the C-terminus to have approximately the same molecular weight as SEQ ID NO: 7. The amino acid sequence shown in SEQ ID NO: 9 has a predetermined hinge sequence and His tag sequence added to the C-terminus of a sequence in which the 20 domain sequence regions present in the amino acid sequence shown in SEQ ID NO: 7 (with the exception that several amino acid residues at the C-terminus of said regions have been replaced) are repeated four times.

[0045] The z / w value in the amino acid sequence shown in SEQ ID NO: 10 (corresponding to naturally derived fibroin) is 46.8%. The z / w values ​​in the amino acid sequence shown in SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9 are 58.7%, 70.1%, 66.1%, and 70.0%, respectively. Furthermore, the x / y values ​​in the jagged ratio (described later) of 1:1.8 to 11.3 in the amino acid sequences shown in SEQ ID NO: 10, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9 are 15.0%, 15.0%, 93.4%, 92.7%, and 89.8%, respectively.

[0046] The modified fibroin (2-i) may consist of the amino acid sequence shown in SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 or SEQ ID NO:9.

[0047] The modified fibroin of (2-ii) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9. The modified fibroin of (2-ii) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0048] The modified fibroin (2-ii) preferably has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 or SEQ ID NO:9, and has a z / w ratio of 50.9% or more, where z is the total number of amino acid residues in the amino acid sequence consisting of XGX (wherein X represents an amino acid residue other than glycine) contained in REP, and w is the total number of amino acid residues in REP in the domain sequence.

[0049] The second modified fibroin may contain a tag sequence at either or both of the N-terminus and C-terminus, which allows for isolation, immobilization, detection, visualization, etc. of the modified fibroin.

[0050] An example of a tag sequence is an affinity tag that utilizes specific affinity (binding ability, affinity) with other molecules. A specific example of an affinity tag is a histidine tag (His tag). The His tag is a short peptide consisting of about 4 to 10 histidine residues lined up, and has the property of specifically binding to metal ions such as nickel, and can be used to isolate modified fibroin by metal chelating chromatography. A specific example of a tag sequence is the amino acid sequence shown in SEQ ID NO: 11 (an amino acid sequence including a His tag sequence and a hinge sequence).

[0051] Furthermore, tag sequences such as glutathione-S-transferase (GST) that specifically binds to glutathione, and maltose-binding protein (MBP) that specifically binds to maltose can also be used.

[0052] Furthermore, an "epitope tag" utilizing an antigen-antibody reaction can also be used. By adding an antigenic peptide (epitope) as a tag sequence, an antibody against the epitope can be bound. Examples of epitope tags include HA (peptide sequence of influenza virus hemagglutinin) tag, myc tag, and FLAG tag. By using an epitope tag, the modified fibroin can be easily purified with high specificity.

[0053] Furthermore, a tag sequence that can be cleaved with a specific protease can also be used. By treating the protein adsorbed via the tag sequence with the protease, the modified fibroin from which the tag sequence has been cleaved can be recovered.

[0054] More specific examples of modified fibroins containing a tag sequence include (2-iii) modified fibroins containing an amino acid sequence shown in SEQ ID NO: 12 (PRT380), SEQ ID NO: 13 (PRT410), SEQ ID NO: 14 (PRT525) or SEQ ID NO: 15 (PRT799), or (2-iv) an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 or SEQ ID NO: 15.

[0055] The amino acid sequences shown in SEQ ID NO:16 (PRT313), SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 are obtained by adding the amino acid sequence shown in SEQ ID NO:11 (including the His tag sequence and hinge sequence) to the N-terminus of the amino acid sequences shown in SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9, respectively.

[0056] The modified fibroin (2-iii) may consist of the amino acid sequence shown in SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.

[0057] The modified fibroin of (2-iv) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15. The modified fibroin of (2-iv) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0058] The modified fibroin (2-iv) has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 or SEQ ID NO: 15, and preferably has a z / w ratio of 50.9% or more, where z is the total number of amino acid residues in the amino acid sequence consisting of XGX (wherein X represents an amino acid residue other than glycine) contained in REP, and w is the total number of amino acid residues in REP in the domain sequence.

[0059] The second modified fibroin may contain a secretion signal for releasing the protein produced in the recombinant protein production system to the outside of the host. The sequence of the secretion signal can be appropriately set depending on the type of the host.

[0060] The third modified fibroin has a domain sequence different from that of naturally occurring fibroin, as follows: (A) n The third modified fibroin domain sequence has an amino acid sequence with a reduced content of motifs compared to naturally occurring fibroin. n It can be said that it has an amino acid sequence corresponding to the deletion of the motif.

[0061] The third modified fibroin was derived from naturally occurring fibroin (A). n It may have an amino acid sequence corresponding to a 10 to 40% deletion of the motif.

[0062] The third modified fibroin has a domain sequence that is different from that of naturally-occurring fibroin in that it has at least one to three (A) n One (A) per motif n It may have an amino acid sequence corresponding to the deletion of the motif.

[0063] The third modified fibroin has a domain sequence that is at least two consecutive domains from the N-terminus to the C-terminus, as compared with naturally occurring fibroin (A). n Deletion of the motif and one (A) n It may have an amino acid sequence corresponding to repeated deletion of the motif in this order.

[0064] The third modified fibroin has a domain sequence in which at least every third domain from the N-terminus to the C-terminus is (A) n It may have an amino acid sequence corresponding to the deletion of the motif.

[0065] The third modified fibroin has the formula 1: [(A) n Motif-REP] m The domain sequence is represented by the following two adjacent sequences from the N-terminus to the C-terminus: n The number of amino acid residues in the REP of the motif-REP] unit is compared in sequence. When the number of amino acid residues in the REP with the smaller number of amino acid residues is set to 1, the ratio of the number of amino acid residues in the other REP is 1.8 to 11.3 in two adjacent [(A) n When the maximum sum of the numbers of amino acid residues in the motif-REP units is x and the total number of amino acid residues in the domain sequence is y, the amino acid sequence may have an x / y ratio of 20% or more, 30% or more, 40% or more, or 50% or more. (A) n The number of alanine residues relative to the total number of amino acid residues in the motif may be 83% or more, but is preferably 86% or more, more preferably 90% or more, even more preferably 95% or more, and even more preferably 100% (meaning that it is composed only of alanine residues).

[0066] The method of calculating x / y will be described in more detail with reference to Fig. 1. Fig. 1 shows the domain sequence of the modified fibroin excluding the N-terminal sequence and the C-terminal sequence. The domain sequence is as follows from the N-terminal side (left side): (A) n Motif-First REP (50 amino acid residues)-(A) n Motif-Second REP (100 amino acid residues)-(A) n Motif-3 REP (10 amino acid residues)-(A) n Motif-4th REP (20 amino acid residues)-(A) n Motif-5 REP (30 amino acid residues)-(A) n It has a sequence called a motif.

[0067] Two adjacent [(A) n The motif-REP] units are selected sequentially from the N-terminus to the C-terminus, without overlapping. nA motif-REP] unit may be present. In FIG. 1, pattern 1 (comparison of the first REP with the second REP, and comparison of the third REP with the fourth REP), pattern 2 (comparison of the first REP with the second REP, and comparison of the fourth REP with the fifth REP), pattern 3 (comparison of the second REP with the third REP, and comparison of the fourth REP with the fifth REP), and pattern 4 (comparison of the first REP with the second REP) are shown. It should be noted that there are other selection methods.

[0068] Next, for each pattern, select two adjacent [(A) n The number of amino acid residues of each REP in the motif-REP unit is compared. The comparison is performed by determining the ratio of the number of amino acid residues of the other REP to the number of amino acid residues of the one with fewer amino acid residues set as 1. For example, when comparing the first REP (50 amino acid residues) with the second REP (100 amino acid residues), the ratio of the number of amino acid residues of the second REP is 100 / 50=2 when the number of amino acid residues of the first REP with fewer amino acid residues is set as 1. Similarly, when comparing the fourth REP (20 amino acid residues) with the fifth REP (30 amino acid residues), the ratio of the number of amino acid residues of the fifth REP is 30 / 20=1.5 when the number of amino acid residues of the fourth REP with fewer amino acid residues is set as 1.

[0069] In FIG. 1, when the number of amino acid residues of the one with fewer amino acid residues is set to 1, the ratio of the number of amino acid residues of the other one is 1.8 to 11.3 [(A) n The combination of motif-REP] units is shown by a solid line. In this specification, this ratio is called the "Jagged ratio." When the one with the fewer amino acid residues is set to 1, the ratio of the other amino acid residue number is less than 1.8 or more than 11.3 [(A) n The motif-REP] unit pairs are indicated by dashed lines.

[0070] In each pattern, the two adjacent [(A) n Add up the number of amino acid residues in all the motif-REP units (not just REP, but (A) nThe number of amino acid residues in the motif is also x.) Then, the sums are compared, and the sum of the pattern with the largest sum (the maximum sum) is taken as x. In the example shown in Figure 1, the sum of pattern 1 is the largest.

[0071] Next, x / y (%) can be calculated by dividing x by the total number of amino acid residues, y, in the domain sequence.

[0072] In the third modified fibroin, x / y is preferably 50% or more, more preferably 60% or more, even more preferably 65% ​​or more, even more preferably 70% or more, even more preferably 75% or more, and particularly preferably 80% or more. There is no particular upper limit for x / y, and it may be, for example, 100% or less. When the knurling ratio is 1:1.9 to 11.3, x / y is preferably 89.6% or more, when the knurling ratio is 1:1.8 to 3.4, x / y is preferably 77.1% or more, when the knurling ratio is 1:1.9 to 8.4, x / y is preferably 75.9% or more, and when the knurling ratio is 1:1.9 to 4.1, x / y is preferably 64.2% or more.

[0073] The third modified fibroin is present multiple times in the domain sequence (A). n In the case of a modified fibroin in which at least seven of the motifs are composed only of alanine residues, x / y is preferably 46.4% or more, more preferably 50% or more, even more preferably 55% or more, even more preferably 60% or more, even more preferably 70% or more, and particularly preferably 80% or more. There is no particular upper limit to x / y, and it is sufficient as long as it is 100% or less.

[0074] Here, the x / y ratio in naturally derived fibroin will be described. First, as described above, fibroins whose amino acid sequence information is registered in NCBI GenBank were confirmed by the method exemplified above, and 663 types of fibroin (of which, 415 types were spider-derived fibroin) were extracted. Of all the extracted fibroins, those with the formula 1: [(A) n Motif-REP] m The ratio x / y was calculated by the above-mentioned calculation method from the amino acid sequence of naturally occurring fibroin composed of the domain sequence represented by the following formula: As a result, the ratio x / y in all naturally occurring fibroins was less than 64.2% (the highest was 64.14%).

[0075] The third modified fibroin is, for example, (A) such that x / y is 64.2% or more based on the gene sequence of a cloned naturally occurring fibroin. n For example, one or more of the (A) motifs may be deleted from the amino acid sequence of naturally occurring fibroin so that x / y is 64.2% or more. n Alternatively, the amino acid sequence corresponding to the deletion of the motif may be designed and a nucleic acid encoding the designed amino acid sequence may be chemically synthesized. In either case, the amino acid sequence of the naturally occurring fibroin may be selected from (A) n In addition to modifications corresponding to the deletion of a motif, further modifications to the amino acid sequence corresponding to the substitution, deletion, insertion and / or addition of one or more amino acid residues may be made.

[0076] More specific examples of the third modified fibroin include (3-i) a modified fibroin comprising an amino acid sequence shown in SEQ ID NO: 17 (Met-PRT399), SEQ ID NO: 7 (Met-PRT410), SEQ ID NO: 8 (Met-PRT525) or SEQ ID NO: 9 (Met-PRT799), or (3-ii) an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 17, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9.

[0077] The modified fibroin (3-i) will be described. The amino acid sequence shown in SEQ ID NO: 17 is a sequence of the amino acid sequence shown in SEQ ID NO: 10 (Met-PRT313) corresponding to naturally-occurring fibroin, which is obtained by subtracting every third amino acid from the N-terminus to the C-terminus. n The motif was deleted, and the C-terminal sequence was further amplified with [(A) n The amino acid sequence shown in SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9 is as described for the second modified fibroin.

[0078] The value of x / y in the jagged ratio of 1:1.8-11.3 of the amino acid sequence shown in SEQ ID NO:10 (corresponding to naturally derived fibroin) is 15.0%. The values ​​of x / y in the amino acid sequence shown in SEQ ID NO:17 and the amino acid sequence shown in SEQ ID NO:7 are both 93.4%. The value of x / y in the amino acid sequence shown in SEQ ID NO:8 is 92.7%. The value of x / y in the amino acid sequence shown in SEQ ID NO:9 is 89.8%. The values ​​of z / w in the amino acid sequences shown in SEQ ID NO:10, SEQ ID NO:17, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9 are 46.8%, 56.2%, 70.1%, 66.1% and 70.0%, respectively.

[0079] The modified fibroin (3-i) may consist of the amino acid sequence shown in SEQ ID NO: 17, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9.

[0080] The modified fibroin of (3-ii) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 17, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9. The modified fibroin of (3-ii) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0081] The modified fibroin (3-ii) has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 17, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9, and has two adjacent amino acids [(A) n The number of amino acid residues in the REP of the motif-REP] unit is compared in sequence. When the number of amino acid residues in the REP with the fewer amino acid residues is set to 1, the ratio of the number of amino acid residues in the other REP is 1.8 to 11.3 (the jagged ratio is 1:1.8 to 11.3) between two adjacent [(A) n When the maximum sum of the numbers of amino acid residues in the motif-REP units is x and the total number of amino acid residues in the domain sequence is y, it is preferable that x / y is 64.2% or more.

[0082] The third modified fibroin may contain the above-mentioned tag sequence at either or both of the N-terminus and C-terminus.

[0083] More specific examples of modified fibroins containing a tag sequence include (3-iii) modified fibroins containing an amino acid sequence shown in SEQ ID NO: 18 (PRT399), SEQ ID NO: 13 (PRT410), SEQ ID NO: 14 (PRT525) or SEQ ID NO: 15 (PRT799), or (3-iv) an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 18, SEQ ID NO: 13, SEQ ID NO: 14 or SEQ ID NO: 15.

[0084] The amino acid sequences shown in SEQ ID NO:18, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 are obtained by adding the amino acid sequence shown in SEQ ID NO:11 (including a His tag sequence and a hinge sequence) to the N-terminus of the amino acid sequences shown in SEQ ID NO:17, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9, respectively.

[0085] The modified fibroin (3-iii) may consist of the amino acid sequence shown in SEQ ID NO:18, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.

[0086] The modified fibroin of (3-iv) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 18, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15. The modified fibroin of (3-iv) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0087] The modified fibroin (3-iv) has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 18, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, and has two adjacent amino acids [(A) n The number of amino acid residues in the REP of the motif-REP] unit is compared in sequence. When the number of amino acid residues in the REP with the smaller number of amino acid residues is set to 1, the ratio of the number of amino acid residues in the other REP is 1.8 to 11.3 in two adjacent [(A) n When the maximum sum of the numbers of amino acid residues in the motif-REP units is x and the total number of amino acid residues in the domain sequence is y, it is preferable that x / y is 64.2% or more.

[0088] The third modified fibroin may contain a secretion signal for releasing the protein produced in the recombinant protein production system to the outside of the host. The sequence of the secretion signal can be appropriately set depending on the type of the host.

[0089] The fourth modified fibroin has a domain sequence different from that of naturally occurring fibroin, as follows: (A) n In addition to the reduced content of the motif, the fourth modified fibroin has an amino acid sequence with a reduced content of glycine residues. The fourth modified fibroin domain sequence has at least one or more (A) nIn addition to the deletion of the motif, the fourth modified fibroin has an amino acid sequence in which at least one or more glycine residues in REP have been replaced with another amino acid residue. That is, the fourth modified fibroin is a modified fibroin having both the characteristics of the second modified fibroin and the third modified fibroin described above. Specific aspects are as described for the second modified fibroin and the third modified fibroin.

[0090] More specific examples of the fourth modified fibroin include (4-i) modified fibroin containing an amino acid sequence shown in SEQ ID NO: 7 (Met-PRT410), SEQ ID NO: 8 (Met-PRT525), SEQ ID NO: 9 (Met-PRT799), SEQ ID NO: 13 (PRT410), SEQ ID NO: 14 (PRT525) or SEQ ID NO: 15 (PRT799), or (4-ii) modified fibroin containing an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 13, SEQ ID NO: 14 or SEQ ID NO: 15. Specific embodiments of modified fibroin containing the amino acid sequence shown in SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 13, SEQ ID NO: 14 or SEQ ID NO: 15 are as described above.

[0091] The fifth modified fibroin may have an amino acid sequence whose domain sequence includes a localized region of high hydrophobicity index, corresponding to the replacement of one or more amino acid residues in REP with amino acid residues with a high hydrophobicity index and / or the insertion of one or more amino acid residues with a high hydrophobicity index into REP, compared to naturally occurring fibroin.

[0092] The region with a locally high hydrophobicity index is preferably composed of 2 to 4 consecutive amino acid residues.

[0093] The amino acid residue with a high hydrophobicity index is more preferably an amino acid residue selected from isoleucine (I), valine (V), leucine (L), phenylalanine (F), cysteine ​​(C), methionine (M) and alanine (A).

[0094] The fifth modified fibroin may, in addition to modifications corresponding to the replacement of one or more amino acid residues in REP with amino acid residues having a high hydrophobicity index and / or the insertion of one or more amino acid residues having a high hydrophobicity index into REP, have further modifications in the amino acid sequence corresponding to the substitution, deletion, insertion and / or addition of one or more amino acid residues compared to the naturally occurring fibroin.

[0095] The fifth modified fibroin can be obtained, for example, by substituting one or more hydrophilic amino acid residues (e.g., amino acid residues with a negative hydrophobicity index) in REP from the gene sequence of cloned naturally-derived fibroin with hydrophobic amino acid residues (e.g., amino acid residues with a positive hydrophobicity index) and / or by inserting one or more hydrophobic amino acid residues into REP. Alternatively, for example, it can be obtained by designing an amino acid sequence corresponding to the substitution of one or more hydrophilic amino acid residues in REP with hydrophobic amino acid residues from the amino acid sequence of naturally-derived fibroin and / or the insertion of one or more hydrophobic amino acid residues into REP, and chemically synthesizing a nucleic acid encoding the designed amino acid sequence. In either case, in addition to the modification corresponding to the substitution of one or more hydrophilic amino acid residues in REP with hydrophobic amino acid residues from the amino acid sequence of naturally-derived fibroin and / or the insertion of one or more hydrophobic amino acid residues into REP, the amino acid sequence may be modified by further substituting, deleting, inserting and / or adding one or more amino acid residues.

[0096] The fifth modified fibroin has the formula 1: [(A) n Motif-REP] m and (A) is located at the most C-terminal end. nIn all REPs contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence, the total number of amino acid residues contained in a region in which the average hydrophobicity index of four consecutive amino acid residues is 2.6 or more is defined as p, and the region located most C-terminally is defined as (A). n The domain may have an amino acid sequence in which p / q is 6.2% or more, where q is the total number of amino acid residues contained in the domain sequence excluding the sequence from the motif to the C-terminus of the domain sequence.

[0097] The hydrophobicity index of amino acid residues is determined by a known index (Hydropathy index: Kyte J, & Doolittle R (1982) "A simple method for displaying the hydropathic character of a protein", J. Mol. Biol., 157, pp. 105-132). Specifically, the hydrophobicity index (hereinafter also referred to as "HI") of each amino acid is as shown in Table 1 below.

[0098] [Table 1]

[0099] The method of calculating p / q will be described in more detail. The calculation is carried out using the formula 1: [(A) n Motif-REP] m (A) is located at the C-terminal end of the domain sequence represented by nA sequence excluding the sequence from the motif to the C-terminus of the domain sequence (hereinafter referred to as "sequence A") is used. First, the average value of the hydrophobicity index of four consecutive amino acid residues is calculated for all REPs included in sequence A. The average value of the hydrophobicity index is calculated by dividing the sum of the HI of each amino acid residue included in the four consecutive amino acid residues by 4 (the number of amino acid residues). The average value of the hydrophobicity index is calculated for all four consecutive amino acid residues (each amino acid residue is used to calculate the average value 1 to 4 times). Next, a region in which the average value of the hydrophobicity index of the four consecutive amino acid residues is 2.6 or more is specified. Even if a certain amino acid residue corresponds to a plurality of "four consecutive amino acid residues with an average value of the hydrophobicity index of 2.6 or more", it is included as one amino acid residue in the region. The total number of amino acid residues included in the region is p. The total number of amino acid residues included in sequence A is q.

[0100] For example, if 20 "4 consecutive amino acid residues with an average hydrophobicity index of 2.6 or more" are extracted (without overlaps), the region with the average hydrophobicity index of the consecutive 4 amino acid residues of 2.6 or more contains 20 consecutive 4 amino acid residues (without overlaps), and p is 20×4=80. Also, for example, if two "4 consecutive amino acid residues with an average hydrophobicity index of 2.6 or more" overlap by one amino acid residue, the region with the average hydrophobicity index of the consecutive 4 amino acid residues of 2.6 or more contains 7 amino acid residues (p=2×4-1=7, where "-1" is the deduction of the overlap). For example, in the case of the domain sequence shown in FIG. 2, there are 7 "4 consecutive amino acid residues with an average hydrophobicity index of 2.6 or more" without overlaps, and p is 7×4=28. For example, in the case of the domain sequence shown in FIG. 2, q is 4+50+4+40+4+10+4+20+4+30=170 ((A) at the end of the C-terminus). n (The motif is not included.) Next, p / q (%) can be calculated by dividing p by q. In the case of Figure 2, it is 28 / 170 = 16.47%.

[0101] In the fifth modified fibroin, p / q is preferably 6.2% or more, more preferably 7% or more, even more preferably 10% or more, even more preferably 20% or more, and even more preferably 30% or more. The upper limit of p / q is not particularly limited, but may be, for example, 45% or less.

[0102] The fifth modified fibroin can be obtained, for example, by modifying the amino acid sequence of a cloned naturally-derived fibroin to include a region with a high hydrophobic index locally by substituting one or more hydrophilic amino acid residues (e.g., amino acid residues with a negative hydrophobic index) in the REP with hydrophobic amino acid residues (e.g., amino acid residues with a positive hydrophobic index) and / or inserting one or more hydrophobic amino acid residues into the REP so as to satisfy the above-mentioned p / q condition. Alternatively, for example, the fifth modified fibroin can be obtained by designing an amino acid sequence that satisfies the above-mentioned p / q condition from the amino acid sequence of a naturally-derived fibroin and chemically synthesizing a nucleic acid encoding the designed amino acid sequence. In either case, in addition to the modification corresponding to the substitution of one or more amino acid residues in the REP with amino acid residues with a high hydrophobic index and / or the insertion of one or more amino acid residues with a high hydrophobic index into the REP, modifications corresponding to the substitution, deletion, insertion and / or addition of one or more amino acid residues may be performed.

[0103] The amino acid residues having a high hydrophobicity index are not particularly limited, but are preferably isoleucine (I), valine (V), leucine (L), phenylalanine (F), cysteine ​​(C), methionine (M) and alanine (A), and more preferably valine (V), leucine (L) and isoleucine (I).

[0104] A more specific example of the fifth modified fibroin includes (5-i) a modified fibroin comprising an amino acid sequence shown in SEQ ID NO: 19 (Met-PRT720), SEQ ID NO: 20 (Met-PRT665) or SEQ ID NO: 21 (Met-PRT666), or (5-ii) an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 19, SEQ ID NO: 20 or SEQ ID NO: 21.

[0105] The modified fibroin of (5-i) will be described. The amino acid sequence shown in SEQ ID NO: 19 is obtained by inserting an amino acid sequence (VLI) consisting of three amino acid residues at every other REP at two positions, excluding the domain sequence at the C-terminus, and further substituting some glutamine (Q) residues with serine (S) residues, and deleting some amino acids at the C-terminus, in the amino acid sequence shown in SEQ ID NO: 7 (Met-PRT410). The amino acid sequence shown in SEQ ID NO: 20 is obtained by inserting an amino acid sequence (VLI) consisting of three amino acid residues at every other REP at one position, in the amino acid sequence shown in SEQ ID NO: 8 (Met-PRT525). The amino acid sequence shown in SEQ ID NO: 21 is obtained by inserting an amino acid sequence (VLI) consisting of three amino acid residues at every other REP at two positions, in the amino acid sequence shown in SEQ ID NO: 8.

[0106] The modified fibroin (5-i) may consist of the amino acid sequence shown in SEQ ID NO:19, SEQ ID NO:20 or SEQ ID NO:21.

[0107] The modified fibroin of (5-ii) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21. The modified fibroin of (5-ii) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0108] The modified fibroin (5-ii) has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21 and is located at the most C-terminal side (A). n In all REPs contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence, the total number of amino acid residues contained in the region in which the average hydrophobicity index of four consecutive amino acid residues is 2.6 or more is defined as p, and the region located most C-terminally is defined as (A). n When the total number of amino acid residues contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence is defined as q, it is preferable that p / q is 6.2% or more.

[0109] The fifth modified fibroin may contain a tag sequence at either or both of the N-terminus and C-terminus.

[0110] More specific examples of modified fibroins containing a tag sequence include (5-iii) modified fibroins containing an amino acid sequence shown in SEQ ID NO: 22 (PRT720), SEQ ID NO: 23 (PRT665) or SEQ ID NO: 24 (PRT666), or (5-iv) an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 22, SEQ ID NO: 23 or SEQ ID NO: 24.

[0111] The amino acid sequences shown in SEQ ID NO: 22, SEQ ID NO: 23 and SEQ ID NO: 24 are obtained by adding the amino acid sequence shown in SEQ ID NO: 11 (including the His tag sequence and hinge sequence) to the N-terminus of the amino acid sequences shown in SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21, respectively.

[0112] The modified fibroin (5-iii) may consist of the amino acid sequence shown in SEQ ID NO:22, SEQ ID NO:23 or SEQ ID NO:24.

[0113] The modified fibroin (5-iv) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 22, SEQ ID NO: 23, or SEQ ID NO: 24. The modified fibroin (5-iv) also ... n Motif-REP] m It is a protein comprising a domain sequence represented by the following formula: The sequence identity is preferably 95% or more.

[0114] The modified fibroin (5-iv) has a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 22, SEQ ID NO: 23, or SEQ ID NO: 24 and is located at the most C-terminal side (A). n In all REPs contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence, the total number of amino acid residues contained in the region in which the average hydrophobicity index of four consecutive amino acid residues is 2.6 or more is defined as p, and the region located most C-terminally is defined as (A). n When the total number of amino acid residues contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence is defined as q, it is preferable that p / q is 6.2% or more.

[0115] The fifth modified fibroin may contain a secretion signal for releasing the protein produced in the recombinant protein production system to the outside of the host. The sequence of the secretion signal can be appropriately set depending on the type of the host.

[0116] The sixth modified fibroin has an amino acid sequence with a reduced content of glutamine residues compared to naturally occurring fibroin.

[0117] It is preferable that the sixth modified fibroin contains at least one motif selected from the GGX motif and the GPGXX motif in the amino acid sequence of REP.

[0118] When the sixth modified fibroin contains a GPGXX motif in the REP, the GPGXX motif content is usually 1% or more, may be 5% or more, and is preferably 10% or more. There is no particular upper limit to the GPGXX motif content, and it may be 50% or less, or may be 30% or less.

[0119] As used herein, the "GPGXX motif content" is a value calculated by the following method. Formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n In a fibroin (modified fibroin or naturally-occurring fibroin) containing a domain sequence represented by the motif, (A) is located at the most C-terminal side. n For all REPs contained in the sequence excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence, the total number of GPGXX motifs contained in that region is multiplied by three (i.e., the total number of G and P in the GPGXX motif), and the number s is the number located at the most C-terminus side (A). n The sequence from the motif to the C-terminus of the domain sequence is removed from the domain sequence, and further (A) n When the total number of amino acid residues of all REPs excluding the motif is t, the GPGXX motif content is calculated as s / t.

[0120] In calculating the GPGXX motif content, the most C-terminal (A) n The target sequence is the sequence excluding the sequence from the motif to the C-terminus of the domain sequence, which is the sequence located at the most C-terminus (A). nThe "sequence from the motif to the C-terminus of the domain sequence" (sequence corresponding to REP) may contain sequences with low correlation with sequences characteristic of fibroin, and when m is small (i.e., when the domain sequence is short), this affects the calculation results of the GPGXX motif content, so this is to eliminate this influence. Note that when the "GPGXX motif" is located at the C-terminus of REP, even if "XX" is, for example, "AA", it is treated as a "GPGXX motif".

[0121] FIG. 3 is a schematic diagram showing the domain sequence of a modified fibroin. A method for calculating the GPGXX motif content will be specifically described with reference to FIG. 3. First, the domain sequence of the modified fibroin shown in FIG. n Motif-REP] m -(A) n In the 1990s, all REPs were located at the C-terminus (A) n Since the number of GPGXX motifs is 7, s is 7 × 3 = 21. Similarly, all REPs are included in the "domain sequence excluding the sequence from the motif to the C-terminus of the domain sequence" (the sequence shown as "Region A" in Figure 3). n Since the domain sequence is excluded from the sequence from the motif to the C-terminus of the domain sequence (the sequence shown as "Region A" in Figure 3), the sequence is further divided into (A) n The total number of amino acid residues in all REPs excluding motifs, t, is 50 + 40 + 10 + 20 + 30 = 150. Next, s / t (%) can be calculated by dividing s by t, which is 21 / 150 = 14.0% in the case of the modified fibroin in Figure 3.

[0122] The sixth modified fibroin preferably has a glutamine residue content of 9% or less, more preferably 7% or less, even more preferably 4% or less, and particularly preferably 0%.

[0123] In the present specification, the "glutamine residue content" is a value calculated by the following method. Formula 1: [(A)n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n In a fibroin (modified fibroin or naturally-occurring fibroin) containing a domain sequence represented by the motif, (A) is located at the most C-terminal side. n In the sequence obtained by removing the sequence from the motif to the C-terminus of the domain sequence from the domain sequence (the sequence corresponding to "Region A" in Figure 3), the total number of glutamine residues contained in the region is defined as u, and the number of glutamine residues located at the most C-terminus side (A) is defined as n The sequence from the motif to the C-terminus of the domain sequence is removed from the domain sequence, and further (A) n When the total number of amino acid residues of all REPs excluding the motif is t, the glutamine residue content is calculated as u / t. n The reason for targeting "the sequence obtained by excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence" is the same as that mentioned above.

[0124] The sixth modified fibroin may have an amino acid sequence whose domain sequence corresponds to the deletion or substitution of one or more glutamine residues in the REP, compared to a naturally occurring fibroin.

[0125] The "other amino acid residue" may be any amino acid residue other than glutamine residue, but is preferably an amino acid residue with a higher hydrophobicity index than glutamine residue. The hydrophobicity indexes of amino acid residues are shown in Table 1.

[0126] As shown in Table 1, examples of amino acid residues having a higher hydrophobicity index than glutamine residues include amino acid residues selected from isoleucine (I), valine (V), leucine (L), phenylalanine (F), cysteine ​​(C), methionine (M), alanine (A), glycine (G), threonine (T), serine (S), tryptophan (W), tyrosine (Y), proline (P) and histidine (H). Among these, amino acid residues selected from isoleucine (I), valine (V), leucine (L), phenylalanine (F), cysteine ​​(C), methionine (M) and alanine (A) are more preferred, and amino acid residues selected from isoleucine (I), valine (V), leucine (L) and phenylalanine (F) are even more preferred.

[0127] In the sixth modified fibroin, the hydrophobicity of REP is preferably -0.8 or more, more preferably -0.7 or more, even more preferably 0 or more, even more preferably 0.3 or more, and particularly preferably 0.4 or more. There is no particular upper limit to the hydrophobicity of REP, and it may be 1.0 or less, or 0.7 or less.

[0128] In this specification, the "hydrophobicity of REP" is a value calculated by the following method. Formula 1: [(A) n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n In a fibroin (modified fibroin or naturally-occurring fibroin) containing a domain sequence represented by the motif, (A) is located at the most C-terminal side. n For all REPs contained in the sequence obtained by removing the sequence from the motif to the C-terminus of the domain sequence (the sequence corresponding to "Region A" in Figure 3), the sum of the hydrophobicity indexes of each amino acid residue in that region is v, and the REP located at the most C-terminus side (A) n The sequence from the motif to the C-terminus of the domain sequence is removed from the domain sequence, and further (A) nWhen the total number of amino acid residues of all REPs excluding the motif is t, the hydrophobicity of the REP is calculated as v / t. n The reason for targeting "the sequence obtained by excluding the sequence from the motif to the C-terminus of the domain sequence from the domain sequence" is the same as that mentioned above.

[0129] The sixth modified fibroin may have a domain sequence that, compared to a naturally occurring fibroin, is modified by the deletion of one or more glutamine residues in the REP and / or the replacement of one or more glutamine residues in the REP with other amino acid residues, and may also have an amino acid sequence modification that is equivalent to the substitution, deletion, insertion and / or addition of one or more amino acid residues.

[0130] The sixth modified fibroin can be obtained, for example, by deleting one or more glutamine residues in REP from the gene sequence of a cloned naturally-occurring fibroin and / or substituting one or more glutamine residues in REP with other amino acid residues. It can also be obtained, for example, by designing an amino acid sequence corresponding to the deletion of one or more glutamine residues in REP from the amino acid sequence of a naturally-occurring fibroin and / or the substitution of one or more glutamine residues in REP with other amino acid residues, and chemically synthesizing a nucleic acid encoding the designed amino acid sequence.

[0131] More specific examples of the sixth modified fibroin include (6-i) modified fibroin having an amino acid sequence shown in SEQ ID NO: 25 (Met-PRT888), SEQ ID NO: 26 (Met-PRT965), SEQ ID NO: 27 (Met-PRT889), SEQ ID NO: 28 (Met-PRT916), SEQ ID NO: 29 (Met-PRT918), SEQ ID NO: 30 (Met-PRT699), SEQ ID NO: 31 (Met-PRT698), SEQ ID NO: 32 (Met-PRT966), SEQ ID NO: 41 (Met-PRT917) or SEQ ID NO: 42 (Met-PRT1028), or (6-ii) modified fibroin having an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 41 or SEQ ID NO: 42.

[0132] The modified fibroin (6-i) will be described. The amino acid sequence shown in SEQ ID NO: 25 is obtained by substituting all QQ in the amino acid sequence shown in SEQ ID NO: 7 (Met-PRT410) with VL. The amino acid sequence shown in SEQ ID NO: 26 is obtained by substituting all QQ in the amino acid sequence shown in SEQ ID NO: 7 with TS, and substituting the remaining Q with A. The amino acid sequence shown in SEQ ID NO: 27 is obtained by substituting all QQ in the amino acid sequence shown in SEQ ID NO: 7 with VL, and substituting the remaining Q with I. The amino acid sequence shown in SEQ ID NO: 28 is obtained by substituting all QQ in the amino acid sequence shown in SEQ ID NO: 7 with VI, and substituting the remaining Q with L. The amino acid sequence shown in SEQ ID NO: 29 is obtained by substituting all QQ in the amino acid sequence shown in SEQ ID NO: 7 with VF, and substituting the remaining Q with I.

[0133] The amino acid sequence shown in SEQ ID NO: 30 is the amino acid sequence shown in SEQ ID NO: 8 (Met-PRT525) in which all QQ are replaced with VL. The amino acid sequence shown in SEQ ID NO: 31 is the amino acid sequence shown in SEQ ID NO: 8 in which all QQ are replaced with VL and the remaining Q is replaced with I.

[0134] The amino acid sequence shown in SEQ ID NO:32 is a sequence in which the 20 domain sequence region present in the amino acid sequence shown in SEQ ID NO:7 (Met-PRT410) is repeated twice, with all QQs replaced with VF and the remaining Qs replaced with I.

[0135] The amino acid sequence shown in SEQ ID NO:41 (Met-PRT917) is obtained by replacing all QQ in the amino acid sequence shown in SEQ ID NO:7 with LI and the remaining Q with V. The amino acid sequence shown in SEQ ID NO:42 (Met-PRT1028) is obtained by replacing all QQ in the amino acid sequence shown in SEQ ID NO:7 with IF and the remaining Q with T.

[0136] The amino acid sequences shown in SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:41 and SEQ ID NO:42 all have a glutamine residue content of 9% or less (Table 2).

[0137] [Table 2]

[0138] The modified fibroin (6-i) may consist of the amino acid sequence shown in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 41 or SEQ ID NO: 42.

[0139] The modified fibroin of (6-ii) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 41, or SEQ ID NO: 42. The modified fibroin of (6-ii) also contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 41, or SEQ ID NO: 42. The modified fibroin of (6 n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) nA protein containing a domain sequence represented by a motif. The sequence identity is required to be 95% or more. preferable.

[0140] The modified fibroin (6-ii) preferably has a glutamine residue content of 9% or less, and the modified fibroin (6-ii) preferably has a GPGXX motif content of 10% or more.

[0141] The sixth modified fibroin may contain a tag sequence at either or both of the N-terminus and C-terminus, which allows the modified fibroin to be isolated, immobilized, detected, visualized, and the like.

[0142] More specific examples of modified fibroins containing a tag sequence include (6-iii) modified fibroins containing the amino acid sequence shown in SEQ ID NO: 33 (PRT888), SEQ ID NO: 34 (PRT965), SEQ ID NO: 35 (PRT889), SEQ ID NO: 36 (PRT916), SEQ ID NO: 37 (PRT918), SEQ ID NO: 38 (PRT699), SEQ ID NO: 39 (PRT698), SEQ ID NO: 40 (PRT966), SEQ ID NO: 43 (PRT917) or SEQ ID NO: 44 (PRT1028), or (6-iv) modified fibroins containing an amino acid sequence having 90% or more sequence identity to the amino acid sequence shown in SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 43 or SEQ ID NO: 44.

[0143] The amino acid sequences shown in SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:43 and SEQ ID NO:44 are obtained by adding the amino acid sequence shown in SEQ ID NO:11 (including a His tag sequence and a hinge sequence) to the N-terminus of the amino acid sequences shown in SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:41 and SEQ ID NO:42, respectively. Since only a tag sequence is added to the N-terminus, there is no change in the glutamine residue content, and the amino acid sequences shown in SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:43 and SEQ ID NO:44 all have a glutamine residue content of 9% or less (Table 3).

[0144] [Table 3]

[0145] The modified fibroin (6-iii) may consist of the amino acid sequence shown in SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 43 or SEQ ID NO: 44.

[0146] The modified fibroin of (6-iv) contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 43, or SEQ ID NO: 44. The modified fibroin of (6-iv) also contains an amino acid sequence having 90% or more sequence identity with the amino acid sequence shown in SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 43, or SEQ ID NO: 44. The modified fibroin of (6 n Motif-REP] m Or formula 2: [(A) n Motif-REP] m -(A) n It is a protein containing a domain sequence represented by the motif. The sequence identity is preferably 95% or more.

[0147] The modified fibroin (6-iv) preferably has a glutamine residue content of 9% or less, and the modified fibroin (6-iv) preferably has a GPGXX motif content of 10% or more.

[0148] The sixth modified fibroin may contain a secretion signal for releasing the protein produced in the recombinant protein production system to the outside of the host. The sequence of the secretion signal can be appropriately selected depending on the type of the host.

[0149] The modified fibroin may be a modified fibroin having at least two or more of the characteristics of the first modified fibroin, the second modified fibroin, the third modified fibroin, the fourth modified fibroin, the fifth modified fibroin, and the sixth modified fibroin.

[0150] The modified fibroin may be either hydrophilic or hydrophobic. The hydrophobic modified fibroin is a modified fibroin in which the sum of the hydrophobicity index (HI) of all amino acid residues constituting the modified fibroin is calculated and then the sum is divided by the total number of amino acid residues, resulting in an average HI of 0 or more. The hydrophobicity index is as shown in Table 1. The hydrophilic modified fibroin is a modified fibroin in which the average HI is less than 0. From the viewpoint of being more excellent in shrinkage resistance against moisture, the average hydrophobicity index (HI) of the modified fibroin according to the present embodiment is preferably -1.3 or more, preferably -0.8 or more, preferably more than -0.8, preferably -0.7 or more, preferably -0.6 or more, more preferably -0.5 or more, preferably -0.4 or more, preferably -0.3 or more, preferably -0.2 or more, preferably -0.1 or more, more preferably 0 or more, more preferably 0.1 or more, more preferably 0.2 or more, even more preferably 0.3 or more, particularly preferably 0.4 or more. The average hydrophobicity index (HI) may be 1.5 or less, 1.4 or less, or 1.3 or less.

[0151] An example of the hydrophobic modified fibroin is the modified fibroin No. 6 described above. More specific examples of the hydrophobic modified fibroin include modified fibroins containing the amino acid sequence shown in SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, or SEQ ID NO:43, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, or SEQ ID NO:44.

[0152] Examples of the hydrophilic modified fibroin include the above-mentioned first modified fibroin, second modified fibroin, third modified fibroin, fourth modified fibroin, and fifth modified fibroin. More specific examples of the hydrophilic modified fibroin include modified fibroins containing the amino acid sequence shown in SEQ ID NO: 4, the amino acid sequence shown in SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, the amino acid sequence shown in SEQ ID NO: 13, SEQ ID NO: 11, SEQ ID NO: 14, or SEQ ID NO: 15, the amino acid sequence shown in SEQ ID NO: 18, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, the amino acid sequence shown in SEQ ID NO: 17, SEQ ID NO: 11, SEQ ID NO: 14, or SEQ ID NO: 15, the amino acid sequence shown in SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21.

[0153] (Method of producing modified fibroin) The modified fibroin according to any of the above embodiments can be produced, for example, by expressing the nucleic acid by a host transformed with an expression vector having a nucleic acid sequence encoding the modified fibroin and one or more regulatory sequences operably linked to the nucleic acid sequence.

[0154] The method for producing a nucleic acid encoding a modified fibroin is not particularly limited. For example, the nucleic acid can be produced by amplifying and cloning a gene encoding a natural fibroin using polymerase chain reaction (PCR) or the like, and modifying the gene using genetic engineering techniques, or by chemical synthesis. The method for chemically synthesizing a nucleic acid is also not particularly limited. For example, a gene can be chemically synthesized by linking oligonucleotides automatically synthesized using AKTA oligopilot plus 10 / 100 (GE Healthcare Japan, Inc.) or the like using PCR or the like, based on the amino acid sequence information of fibroin obtained from the NCBI web database or the like. In this case, in order to facilitate purification and / or confirmation of the modified fibroin, a nucleic acid encoding a modified fibroin having an amino acid sequence in which an amino acid sequence consisting of an initiation codon and a His10 tag is added to the N-terminus of the above amino acid sequence may be synthesized.

[0155] The regulatory sequence is a sequence (e.g., promoter, enhancer, ribosome binding sequence, transcription termination sequence, etc.) that controls the expression of the modified fibroin in the host, and can be selected appropriately depending on the type of host. The promoter may be an inducible promoter that functions in the host cell and can induce the expression of the modified fibroin. An inducible promoter is a promoter that can control transcription by the presence of an inducer (expression inducer), the absence of a repressor molecule, or physical factors such as an increase or decrease in temperature, osmotic pressure, or pH value.

[0156] The type of expression vector can be appropriately selected according to the type of host, such as a plasmid vector, a virus vector, a cosmid vector, a fosmid vector, an artificial chromosome vector, etc. As the expression vector, one that can autonomously replicate in a host cell or can be integrated into a host chromosome and contains a promoter at a position where a nucleic acid encoding a modified fibroin can be transcribed is preferably used.

[0157] As the host, any of prokaryotes and eukaryotes such as yeast, filamentous fungi, insect cells, animal cells and plant cells can be suitably used.

[0158] Preferred examples of prokaryotic hosts include bacteria belonging to the genera Escherichia, Brevibacillus, Serratia, Bacillus, Microbacterium, Brevibacterium, Corynebacterium, and Pseudomonas. Examples of microorganisms belonging to the genus Escherichia include Escherichia coli. Examples of microorganisms belonging to the genus Brevibacillus include Brevibacillus agri. Examples of microorganisms belonging to the genus Serratia include Serratia liquefaciens. Examples of microorganisms belonging to the genus Bacillus include Bacillus subtilis. Examples of microorganisms belonging to the genus Microbacterium include Microbacterium ammoniaphilum. Examples of microorganisms belonging to the genus Brevibacterium include Brevibacterium divaricatum. Examples of microorganisms belonging to the genus Corynebacterium include Corynebacterium ammoniagenes. An example of the microorganism belonging to the genus Pseudomonas is Pseudomonas putida.

[0159] When a prokaryote is used as the host, vectors for introducing a nucleic acid encoding a modified fibroin include, for example, pBTrp2 (Boehringer Mannheim), pGEX (Pharmacia), pUC18, pBluescriptII, pSupex, pET22b, pCold, pUB110, and pNCO2 (JP Patent Publication No. 2002-238569).

[0160] Examples of eukaryotic hosts include yeasts and filamentous fungi (molds, etc.). Examples of yeasts include yeasts belonging to the genera Saccharomyces, Pichia, and Schizosaccharomyces. Examples of filamentous fungi include filamentous fungi belonging to the genera Aspergillus, Penicillium, and Trichoderma.

[0161] When a eukaryote is used as a host, examples of vectors for introducing a nucleic acid encoding a modified fibroin include YEP13 (ATCC37115) and YEp24 (ATCC37051). Any method for introducing an expression vector into the host cell can be used as long as it is a method for introducing DNA into the host cell. Examples of such methods include a method using calcium ions [Proc. Natl. Acad. Sci. USA, 69, 2110 (1972)], electroporation, spheroplast method, protoplast method, lithium acetate method, and competent method.

[0162] Methods for expressing nucleic acids using a host transformed with an expression vector include direct expression, secretory production, fusion protein expression, etc., in accordance with the methods described in Molecular Cloning, 2nd Edition.

[0163] The modified fibroin can be produced, for example, by culturing a host transformed with an expression vector in a culture medium, producing and accumulating the modified fibroin in the culture medium, and collecting it from the culture medium. The method for culturing the host in the culture medium can be performed according to a method commonly used for culturing the host.

[0164] When the host is a prokaryote such as Escherichia coli or a eukaryote such as yeast, the culture medium may be either a natural medium or a synthetic medium, as long as it contains a carbon source, a nitrogen source, inorganic salts, etc. that can be assimilated by the host and allows efficient cultivation of the host.

[0165] The carbon source may be any that can be assimilated by the transformed microorganism, and may include, for example, carbohydrates such as glucose, fructose, sucrose, and molasses containing these, starch, and starch hydrolysates, organic acids such as acetic acid and propionic acid, and alcohols such as ethanol and propanol. The nitrogen source may include, for example, inorganic acids or ammonium salts of organic acids such as ammonia, ammonium chloride, ammonium sulfate, ammonium acetate, and ammonium phosphate, other nitrogen-containing compounds, as well as peptone, meat extract, yeast extract, corn steep liquor, casein hydrolysate, soybean meal, soybean meal hydrolysate, various fermentation bacteria, and digests thereof. The inorganic salts may include, for example, potassium monophosphate, potassium diphosphate, magnesium phosphate, magnesium sulfate, sodium chloride, ferrous sulfate, manganese sulfate, copper sulfate, and calcium carbonate.

[0166] Cultivation of prokaryotes such as Escherichia coli or eukaryotes such as yeast can be carried out under aerobic conditions, for example, by shaking culture or submerged aeration and stirring culture. The culture temperature is, for example, 15 to 40°C. The culture time is usually 16 hours to 7 days. The pH of the culture medium during culture is preferably maintained at 3.0 to 9.0. The pH of the culture medium can be adjusted using an inorganic acid, an organic acid, an alkaline solution, urea, calcium carbonate, ammonia, or the like.

[0167] During the culture, antibiotics such as ampicillin and tetracycline may be added to the culture medium as necessary. When culturing a microorganism transformed with an expression vector using an inducible promoter as a promoter, an inducer may be added to the medium as necessary. For example, isopropyl-β-D-thiogalactopyranoside or the like may be added to the medium when culturing a microorganism transformed with an expression vector using a lac promoter, and indoleacrylic acid or the like may be added to the medium when culturing a microorganism transformed with an expression vector using a trp promoter.

[0168] The expressed modified fibroin can be isolated and purified by a commonly used method. For example, when the modified fibroin is expressed in a dissolved state within the cells, after the culture is completed, the host cells are collected by centrifugation and suspended in an aqueous buffer solution, and then disrupted by an ultrasonic homogenizer, French press, Manton Gaulin homogenizer, Dyno Mill, or the like to obtain a cell-free extract. From the supernatant obtained by centrifuging the cell-free extract, a purified specimen can be obtained by using, alone or in combination, methods commonly used for isolating and purifying proteins, namely, solvent extraction, salting out with ammonium sulfate or the like, desalting, precipitation with an organic solvent, anion exchange chromatography using resins such as diethylaminoethyl (DEAE)-Sepharose and DIAION HPA-75 (manufactured by Mitsubishi Chemical Corporation), cation exchange chromatography using resins such as S-Sepharose FF (manufactured by Pharmacia), hydrophobic chromatography using resins such as butyl Sepharose and phenyl Sepharose, gel filtration using molecular sieves, affinity chromatography, chromatofocusing, electrophoresis such as isoelectric focusing, and the like.

[0169] Furthermore, when the modified fibroin is expressed by forming an insoluble body within the cells, the host cells are similarly recovered, disrupted, and centrifuged to recover the insoluble body of the modified fibroin as a precipitate fraction. The recovered insoluble body of the modified fibroin can be solubilized with a protein denaturant. After this operation, a purified preparation of the modified fibroin can be obtained by the same isolation and purification method as above. When the modified fibroin is secreted outside the cells, the modified fibroin can be recovered from the culture supernatant. That is, the culture is treated by a method such as centrifugation to obtain a culture supernatant, and a purified preparation can be obtained from the culture supernatant by the same isolation and purification method as above.

[0170] [Spinning solution] The spinning solution (dope solution) according to this embodiment contains a modified fibroin and a solvent.

[0171] The solvent of the spinning dope according to the present embodiment can be any solvent capable of dissolving the modified fibroin, and examples thereof include hexafluoroisopropanol (HFIP), hexafluoroacetone (HFA), dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMA), 1,3-dimethyl-2-imidazolidone (DMI), N-methyl-2-pyrrolidone (NMP), acetonitrile, N-methylmorpholine N-oxide (NMO), and formic acid. From the viewpoint of better solubility of the modified fibroin, hexafluoroisopropanol, dimethyl sulfoxide, and formic acid are more preferred, and dimethyl sulfoxide and formic acid are even more preferred. These organic solvents may contain water. These solvents may be used alone or in combination of two or more.

[0172] The concentration of the modified fibroin in the spinning dope according to the present embodiment is preferably 5 to 40% by weight, more preferably 7 to 40% by weight, more preferably 10 to 40% by weight, more preferably 7 to 35% by weight, more preferably 10 to 35% by weight, more preferably 12 to 35% by weight, more preferably 15 to 35% by weight, more preferably 15 to 30% by weight, even more preferably 20 to 35% by weight, particularly preferably 20 to 30% by weight, and particularly preferably 25 to 35% by weight. When the concentration of the modified fibroin is 5% by weight or more, the productivity is further improved. When the concentration of the modified fibroin is 40% by weight or less, the spinning dope can be discharged from the spinneret more stably, and the productivity is improved.

[0173] If necessary, an inorganic salt may be added to the spinning dope according to the present embodiment. The inorganic salt may function as a dissolution promoter for the modified fibroin. Examples of the inorganic salt include alkali metal halides, alkaline earth metal halides, and alkaline earth metal nitrates. Specific examples of the inorganic salt include lithium carbonate, lithium chloride, calcium chloride, calcium nitrate, lithium bromide, barium bromide, calcium bromide, barium chlorate, sodium perchlorate, lithium perchlorate, barium perchlorate, calcium perchlorate, and magnesium perchlorate. At least one of these inorganic salts may be added to the solvent.

[0174] The method for preparing the spinning dope according to the present embodiment is not particularly limited, and the modified fibroin and the solvent may be mixed in any order. The spinning dope may be stirred or shaken for a certain period of time to promote dissolution. In this case, the spinning dope may be heated to a temperature at which the modified fibroin and the solvent used can be dissolved, if necessary. The spinning dope may be heated to, for example, 30°C or higher, 40°C or higher, 50°C or higher, 60°C or higher, 70°C or higher, 80°C or higher, or 90°C or higher. The upper limit of the heating temperature is, for example, below the boiling point of the solvent.

[0175] The viscosity of the spinning dope according to the present embodiment may be appropriately set depending on the application of the fiber, the spinning method, etc. For example, at 40° C., it may be 1,000 to 35,000 mPa·sec, 1,000 to 30,000 mPa·sec, 1,000 to 20,000 mPa·sec, 3,000 to 20,000 mPa·sec, 5,000 to 30,000 mPa·sec, 5,000 to 15,000 mPa·sec, 5,000 to 12,000 mPa·sec, 5,000 to 10,000 mPa·sec, 7,000 to 30,000 mPa·sec, 7,000 to 12,000 mPa·sec, 10,000 to 30,000 mPa·sec, etc. The viscosity of the spinning dope can be measured, for example, by using an "EMS Viscometer" (trade name) manufactured by Kyoto Electronics Manufacturing Co., Ltd.

[0176] [Raw fiber] The raw fiber according to this embodiment is obtained by spinning the modified fibroin described above, and contains the modified fibroin described above as a main component. The raw fiber according to this embodiment is a fiber before it is irreversibly shrunk after spinning. The fiber diameter of the raw fiber is preferably more than 25 μm.

[0177] The lower limit of the fiber diameter of the raw fiber is preferably more than 25 μm, but may be 28 μm or more, 30 μm or more, 32 μm or more, 34 μm or more, 35 μm or more, 36 μm or more, 38 μm or more, 40 μm or more, 45 μm or more, 50 μm or more, 55 μm or more, or 65 μm or more.

[0178] The upper limit of the fiber diameter of the raw fiber is preferably 120 μm or less, and may be 115 μm or less, 110 μm or less, 105 μm or less, 100 μm or less, 95 μm or less, 90 μm or less, 85 μm or less, 80 μm or less, or 75 μm or less. The fiber diameter of the raw fiber may be more than 25 μm to 120 μm, more than 25 μm to 115 μm, more than 25 μm to 110 μm, more than 25 μm to 105 μm, more than 25 μm to 100 μm, more than 25 μm to 95 μm, more than 25 μm to 90 μm, more than 25 μm to 85 μm, 30 μm to 120 μm, 30 μm to 115 μm, 30 μm to 110 μm, 30 μm to 105 μm, 30 μm to 100 μm, or 30 μm to 95 μm. Alternatively, the thickness may be 30 μm to 90 μm, 30 μm to 85 μm, 35 μm to 120 μm, 35 μm to 115 μm, 35 μm to 110 μm, 35 μm to 105 μm, 35 μm to 100 μm, 35 μm to 95 μm, 35 μm to 90 μm, 35 μm to 85 μm, 40 μm to 120 μm, 40 μm to 115 μm, 40 μm to 110 μm, 40 μm to 105 μm, or 40 μm to 100 μm. Alternatively, it may be 40 μm to 95 μm, 40 μm to 90 μm, 40 μm to 85 μm, 45 μm to 120 μm, 45 μm to 115 μm, 45 μm to 110 μm, 45 μm to 105 μm, 45 μm to 100 μm, 45 μm to 95 μm, 45 μm to 90 μm, 45 μm to 85 μm, 48 μm to 120 μm, 48 μm to 115 μm, 48 μm to 110 μm, or 48 μm to 105 μm. , may be 48 μm to 100 μm, may be 48 μm to 95 μm, may be 48 μm to 90 μm, may be 48 μm to 85 μm, may be 50 μm to 120 μm, may be 50 μm to 115 μm, may be 50 μm to 110 μm, may be 50 μm to 105 μm, may be 50 μm to 100 μm, may be 50 μm to 95 μm, may be 50 μm to 90 μm, may be 50 μm to 85 μm, may be 55 μm to 120 μm, may be 55 μm to 115 μm, may be 55 μm to 110 μm,It may be 55 μm to 105 μm, may be 55 μm to 100 μm, may be 55 μm to 95 μm, may be 55 μm to 90 μm, may be 55 μm to 85 μm, may be 55 μm to 80 μm, may be 60 μm to 120 μm, may be 60 μm to 115 μm, may be 60 μm to 110 μm, may be 60 μm to 105 μm, may be 60 μm to 100 μm, may be 60 μm to 95 μm, may be 60 μm to 90 μm, may be 60 μm to 85 μm, may be 55 μm to 120 μm, may be 55 μm to 115 The fiber diameter may be 55 μm to 110 μm, 55 μm to 105 μm, 55 μm to 100 μm, 55 μm to 95 μm, 55 μm to 90 μm, 55 μm to 85 μm, 65 μm to 120 μm, 65 μm to 115 μm, 65 μm to 110 μm, 65 μm to 105 μm, 65 μm to 100 μm, 65 μm to 95 μm, 65 μm to 90 μm, 65 μm to 85 μm, or 60 μm to 80 μm. By making the fiber diameter more than 25 μm, shrinkage due to contact with moisture can be reduced. By making the fiber diameter 120 μm or less, desolvation during fiber formation can be performed more efficiently.

[0179] [Method of producing raw fiber] [Spinning process] The raw fiber according to the present embodiment can be produced by a known wet spinning method, dry spinning method, dry-wet spinning method, melt spinning method, etc. The raw fiber according to the present embodiment can be produced by using, for example, a spinning apparatus shown in Fig. 4. Preferred spinning methods include wet spinning and dry-wet spinning.

[0180] Fig. 4 is an explanatory diagram showing a schematic diagram of an example of a spinning apparatus for producing raw fiber. The spinning apparatus 10 shown in Fig. 4 is an example of a spinning apparatus for dry / wet spinning, and includes an extrusion device 1, a coagulation bath 20, a washing bath (stretching bath) 21, and a drying device 4 in this order from the upstream side.

[0181] The extrusion device 1 has a storage tank 7, in which a spinning stock solution (dope solution) 6 is stored. A coagulation liquid 11 is stored in a coagulation bath 20. The spinning stock solution 6 is extruded from a spinneret (nozzle) 9 by a gear pump 8 attached to the lower end of the storage tank 7. In a laboratory scale, the spinning stock solution may be filled in a cylinder and extruded from a nozzle using a syringe pump or the like. The extruded spinning stock solution 6 is supplied (introduced) into the coagulation liquid 11 in the coagulation bath 20 through an air gap 19. In the coagulation liquid 11, the solvent is removed from the spinning stock solution, and the modified fibroin is coagulated to form a fibrous coagulate. The fibrous coagulate is then supplied into a washing liquid 12 in a washing bath 21 and stretched. The stretching ratio is determined by the speed ratio between a first nip roller 13 and a second nip roller 14 installed in the washing bath 21. Thereafter, the drawn fibrous coagulate is fed into the drying device 4, dried in the yarn path 22, and wound up by the winder. In this manner, the raw fiber is obtained by the spinning device 10 as a wound product 5 that is finally wound up by the winder. Note that 18a to 18g are yarn guides.

[0182] The coagulation liquid 11 may be any solvent capable of removing the solvent, and examples of the solvent include lower alcohols having 1 to 5 carbon atoms, such as methanol, ethanol, and 2-propanol, and acetone. The coagulation liquid 11 may contain water as appropriate. When a syringe pump having a nozzle with a diameter of 0.1 to 0.6 mm is used as the nozzle 9, the extrusion speed is preferably 0.2 to 6.0 ml / hour, and more preferably 1.4 to 4.0 ml / hour per hole. The distance over which the coagulated modified fibroin passes through the coagulation liquid 11 (effectively, the distance from the yarn guide 18a to the yarn guide 18b) may be a length that allows efficient removal of the solvent, and is, for example, 200 to 500 mm. The take-up speed of the undrawn yarn may be, for example, 1 to 100 m / min, 1 to 20 m / min, and preferably 1 to 3 m / min. When the take-up speed is 1 m / min or more, productivity can be sufficiently increased. When the take-up speed is 100 m / min or less, significant liquid scattering of the solvent can be avoided. The residence time in the coagulation liquid 11 may be a time required for removing the solvent from the spinning dope, and may be, for example, 0.01 to 3 minutes, and preferably 0.05 to 0.15 minutes. Also, drawing (pre-drawing) may be performed in the coagulation liquid 11. The coagulation bath 20 may be provided in multiple stages, and drawing may be performed in each stage or in a specific stage as necessary.

[0183] The shape of the spinneret, the shape of the holes, the number of holes, etc. are not particularly limited, and can be appropriately selected depending on the desired fiber diameter, the number of single filaments, etc.

[0184] When the hole shape of the spinneret is circular, the hole diameter can be exemplified as 0.01 mm or more and 0.6 mm or less. When the hole diameter is 0.01 mm or more, the pressure loss can be reduced and the equipment cost can be suppressed. When the hole diameter is 0.6 mm or less, the necessity of the drawing operation for narrowing the fiber diameter can be reduced and the possibility of the drawing breakage occurring between discharge and take-up can be reduced.

[0185] The temperature of the spinning dope when passing through the spinneret and the temperature of the spinneret are not particularly limited and may be appropriately adjusted depending on the concentration and viscosity of the spinning dope used, the type of organic solvent, etc. The temperature is preferably 30°C to 100°C from the viewpoint of preventing deterioration of the modified fibroin. In addition, the upper limit of the temperature is preferably a temperature below the boiling point of the solvent used from the viewpoint of reducing the possibility of pressure rise due to solvent evaporation and clogging in the piping due to solidification of the spinning dope. This improves process stability.

[0186] The temperature of the solidifying liquid 11 is not particularly limited, but may be 40°C or less, 30°C or less, 25°C or less, 20°C or less, 10°C or less, or 5°C or less. From the viewpoint of workability, cooling costs, etc., it is preferable that the temperature is 0°C or more. The temperature of the solidifying liquid 11 can be adjusted, for example, by using a spinning apparatus 10 having a coagulation bath 20 equipped with a heat exchanger therein and a cooling circulation device. For example, by flowing a medium cooled to a predetermined temperature by a cooling circulation device through the heat exchanger installed in the coagulation bath 20, the temperature can be adjusted to within the above range by heat exchange between the solidifying liquid 11 and the heat exchanger. In this case, more efficient cooling is possible by circulating the solvent used for the solidifying liquid 11 as a medium.

[0187] A plurality of coagulation baths in which the coagulation liquid is stored may be provided.

[0188] After leaving the coagulated modified fibroin (fibrous coagulate), the coagulated modified fibroin (fibrous coagulate) may be wound up on a winder as is, or may be passed through a drying device, dried, and then wound up on a winder.

[0189] The distance that the coagulated modified fibroin (fibrous coagulate) passes through the coagulation liquid may be determined depending on the extrusion speed (discharge speed) of the spinning dope from the nozzle, etc., as long as the solvent can be removed efficiently. The residence time of the coagulated modified fibroin (or the spinning dope) in the coagulation liquid may be determined depending on the distance that the coagulated modified fibroin passes through the coagulation liquid, the extrusion speed of the spinning dope from the nozzle, etc.

[0190] [Stretching process] The method for producing raw fiber of this embodiment may further include a step of stretching the coagulated modified fibroin (fibrous coagulate) (stretching step). Examples of the stretching method include wet heat stretching and dry heat stretching. The stretching step may be carried out, for example, in a coagulation bath 20 or in a cleaning bath 21. The stretching step may also be carried out in air.

[0191] The stretching carried out in the cleaning bath 21 may be so-called wet heat stretching, which is carried out in warm water or in a solution of warm water with an organic solvent added. The temperature of the wet heat stretching is preferably 50 to 90°C. If the temperature is 50°C or higher, the pore size of the yarn can be made small and stable. If the temperature is 90°C or lower, the temperature can be easily set and spinning stability is improved. The temperature is more preferably 75 to 85°C.

[0192] The wet heat drawing can be carried out in hot water, in a solution of hot water with an organic solvent added, or under steam heating. The temperature may be, for example, 40 to 200°C, 50 to 180°C, 50 to 150°C, or 75 to 90°C. The draw ratio in the wet heat drawing may be, for example, 1 to 30 times, 2 to 25 times, 2 to 20 times, 2 to 15 times, 2 to 10 times, 2 to 8 times, 2 to 6 times, or 2 to 4 times, relative to the undrawn yarn (or pre-drawn yarn). However, the draw ratio is not limited as long as it is within a range in which desired fiber thickness, mechanical properties, and other characteristics can be obtained.

[0193] The dry heat drawing can be performed by drawing in air using an apparatus equipped with a heat source such as a contact type hot plate and a non-contact type furnace, but is not particularly limited as long as the apparatus can heat the fiber to a predetermined temperature and draw it at a predetermined ratio. The temperature may be, for example, 100°C to 270°C, 140°C to 230°C, 140°C to 200°C, 160°C to 200°C, or 160°C to 180°C.

[0194] The draw ratio in the hot drawing step may be, for example, 1 to 30 times, 2 to 30 times, 2 to 20 times, or 3 to 15 times, preferably 3 to 10 times, more preferably 3 to 8 times, and even more preferably 4 to 8 times, relative to the undrawn yarn (or pre-drawn yarn). However, the draw ratio is not limited as long as it is within a range in which desired fiber thickness, mechanical properties, and other characteristics can be obtained.

[0195] The stretching step may be a step in which wet heat stretching and dry heat stretching are performed alone, or may be a step in which these are performed in multiple stages or in combination. That is, in the stretching step, wet heat stretching and dry heat stretching may be appropriately combined, such as performing wet heat stretching in the first stage and dry heat stretching in the second stage, or performing wet heat stretching in the first stage, wet heat stretching in the second stage, and dry heat stretching in the third stage.

[0196] The lower limit of the final draw ratio of the raw fiber after the drawing process may be preferably any one of 1, 2, 3, 4, 5, 6, 7, 8, or 9 times, relative to the undrawn yarn (or pre-drawn yarn). The upper limit of the final draw ratio of the raw fiber after the drawing process may be preferably any one of 40, 30, 20, 15, 14, 13, 12, 11, or 10. In addition, for example, the final draw ratio may be 3 to 40, 3 to 30, 5 to 30, 5 to 20, 5 to 15, or 5 to 13. However, the draw ratio is not limited as long as it is within a range in which the desired fiber thickness, mechanical properties, and other characteristics can be obtained. By adjusting the draw ratio, the fiber diameter of the obtained raw fiber can be adjusted to any value.

[0197] Before or after drying, an oil may be applied to the undrawn yarn (or pre-drawn yarn) or drawn yarn, as necessary, for the purpose of imparting antistatic properties, convergence properties, lubricity, etc. The type and amount of oil applied are not particularly limited, and can be appropriately adjusted taking into consideration the application of the fiber and the handleability of the fiber, etc.

[0198] The manufacturing method according to this embodiment may further include a step of filtering the spinning dope before discharging the spinning dope (filtration step), and / or a step of degassing the spinning dope before discharging the spinning dope (defoaming step).

[0199] [Method for producing modified fibroin fiber (shrinkage process)] The modified fibroin fiber according to this embodiment can be produced by a method including a shrinking step of irreversibly shrinking the raw fiber described above. In the shrinking step of irreversibly shrinking the raw fiber, the raw fiber may be irreversibly shrunk by contacting the raw fiber with water, or the raw fiber may be irreversibly shrunk by heating and relaxing the raw fiber. When the raw fiber is irreversibly shrunk by contacting with water, the irreversibly shrunk fiber may be dried and further shrunk.

[0200] [Shrinkage process due to contact with water (contact process)] FIG. 5 is a diagram showing an example of the change in length of raw fiber (fiber containing modified fibroin) due to contact with water. The raw fiber (fiber containing modified fibroin) according to this embodiment has the property of shrinking (primary shrinkage) when it is brought into contact (wetted) with water below the boiling point (length change shown as "primary shrinkage" in FIG. 5). After the primary shrinkage, drying causes further shrinkage (length change shown as "secondary shrinkage" in FIG. 5). After the secondary shrinkage, when it is brought into contact with water again, it expands to a length equal to or close to that before the secondary shrinkage, and thereafter, when drying and wetting are repeated, it repeats shrinkage and expansion at a width similar to that of the secondary shrinkage (width shown as "expansion rate (shrinkage rate)" in FIG. 5). In other words, the primary shrinkage caused by bringing the raw fiber into contact with water is irreversible shrinkage. Therefore, by bringing the raw fiber into contact with water in the shrinkage process, modified fibroin fiber having a shrinkage history that has been irreversibly shrunk according to this embodiment can be obtained. The process of irreversibly shrinking (primary shrinkage) by bringing the raw fiber into contact with water is hereinafter referred to as the "contact process".

[0201] The irreversible shrinkage of the raw fiber (fiber containing modified fibroin) during the contacting step ("primary shrinkage" in Figure 5) is believed to occur for, for example, the following reasons. That is, one reason is believed to be due to the primary structure of the raw fiber (fiber containing modified fibroin), and another reason is believed to be that, for example, in raw fiber (fiber containing modified fibroin) that has residual stress due to stretching or the like during the manufacturing process, water penetrates between or into the fibers, thereby relieving the residual stress.

[0202] In the contacting step, the raw fiber after spinning and before contacting with water is brought into contact with water to make the raw fiber wet. The wet state means that at least a part of the raw fiber is wet with water. This allows the raw fiber to shrink without the application of an external force. This shrinkage is irreversible (corresponding to "primary shrinkage" in Figure 5).

[0203] The temperature of the water brought into contact with the raw fiber in the contacting step may be below the boiling point. This improves the handling property and the workability in the shrinking step. From the viewpoint of sufficiently shortening the shrinking time, the lower limit of the water temperature is preferably 10°C or higher, more preferably 40°C or higher, even more preferably 70°C or higher, even more preferably 80°C or higher, and particularly preferably 90°C or higher. The upper limit of the water temperature is preferably below the boiling point.

[0204] In the contact step, the method of contacting the raw fiber with water is not particularly limited. Examples of the method include a method of immersing the raw fiber in water, a method of spraying water at room temperature or in a state of heated steam or the like onto the raw fiber, and a method of exposing the raw fiber to a high humidity environment filled with water vapor. Among these methods, the method of immersing the raw fiber in water is preferred in the contact step, since it can effectively shorten the shrinkage time and simplify the processing equipment.

[0205] In the contacting step, if the raw fibers are contacted with water in a relaxed state, the raw fibers may not only shrink but also become wavy and crimped. In order to prevent such crimping, the contacting step may be performed in a state where the raw fibers are not relaxed, for example, by contacting the raw fibers with water while pulling them in the fiber axis direction to an extent that no tension is applied.

[0206] (drying process) The method for producing a modified fibroin fiber according to this embodiment may further include a drying step. The drying step is a step in which the raw fiber that has been subjected to the contact step (or the modified fibroin fiber obtained through the contact step) is dried to further shrink it (corresponding to "secondary shrinkage" in FIG. 5). The drying may be, for example, natural drying or forced drying using drying equipment. As the drying equipment, any known contact or non-contact type drying equipment can be used. The drying temperature is also not limited in any way, as long as it is a temperature lower than the temperature at which the modified fibroin contained in the raw fiber is decomposed or the raw fiber is thermally damaged, but is generally a temperature within the range of 20 to 150°C, and preferably a temperature within the range of 50 to 100°C. By keeping the temperature within this range, the fiber is dried more quickly and efficiently without thermal damage to the fiber or decomposition of the modified fibroin contained in the fiber. The drying time is appropriately set according to the drying temperature, etc., and, for example, a time that can eliminate as much as possible the effects of overdrying on the quality and physical properties of the modified fibroin fiber is adopted.

[0207] Fig. 6 is an explanatory diagram showing a schematic diagram of an example of a production apparatus for producing modified fibroin fibers. The production apparatus 40 shown in Fig. 6 is configured to include a feed roller 42 that feeds out the raw fiber, a winder 44 that winds up the modified fibroin fiber 38, a water bath 46 that performs the contacting step, and a dryer 48 that performs the drying step.

[0208] More specifically, the feed roller 42 can be fitted with a wound material of the raw fiber 36, and can continuously and automatically feed the raw fiber 36 from the wound material by the rotation of an electric motor (not shown). The winder 44 can continuously and automatically wind up the modified fibroin fiber 38, which has been produced through a contacting process and a drying process after being fed from the feed roller 42, by the rotation of an electric motor (not shown). Here, the feed speed of the raw fiber 36 by the feed roller 42 and the winding speed of the modified fibroin fiber 38 by the winder 44 can be controlled independently of each other.

[0209] The water bath 46 and the dryer 48 are arranged side by side, respectively on the upstream side and the downstream side in the feeding direction of the raw fiber 36, between the feed roller 42 and the winder 44. The manufacturing apparatus 40 shown in FIG. 6 has relay rollers 50 and 52 that relay the raw fiber 36 traveling from the feed roller 42 toward the winder 44 before and after the contact step.

[0210] The water bath 46 has a heater 54, and water 47 heated by this heater 54 is stored in the water bath 46. A tension roller 56 is also installed in the water bath 46 while being immersed in the water 47. As a result, the raw fiber 36 sent out from the feed roller 42 travels through the water bath 46 toward the winder 44 while being immersed in the water 47 while being wound around the tension roller 56. The immersion time of the raw fiber 36 in the water 47 is appropriately controlled according to the traveling speed of the raw fiber 36.

[0211] The dryer 48 has a pair of hot rollers 58. The pair of hot rollers 58 can wrap the raw fiber 36 that has left the water bath 46 and is traveling toward the winder 44. In this way, the raw fiber 36 immersed in water 47 in the water bath 46 is heated and dried by the pair of hot rollers 58 in the dryer 48, and then is further sent out toward the winder 44.

[0212] When the modified fibroin fiber 38 is manufactured using the manufacturing apparatus 40 having such a structure, first, a wound material of the raw fiber 36 spun using the spinning apparatus 10 shown in FIG. 4 is attached to the feed roller 42. Next, the raw fiber 36 is continuously fed from the feed roller 42 and immersed in water 47 in a water bath 46. At this time, for example, the winding speed of the winder 44 is set slower than the feeding speed of the feed roller 42. This allows the raw fiber 36 to shrink due to contact with the water 47 without being relaxed between the feed roller 42 and the winder 44, thereby preventing the occurrence of crimp. The raw fiber 36 shrinks irreversibly due to contact with the water 47 (corresponding to the "primary shrinkage" in FIG. 5).

[0213] Next, the raw fiber 36 after contact with water 47 (or the modified fibroin fiber 38 produced through contact with water 47) is heated by a pair of hot rollers 58 of a dryer 48. This dries the raw fiber 36 after contact with water 47 (or the modified fibroin fiber 38 produced through contact with water 47) and allows it to be further shrunk (corresponding to the "secondary shrinkage" in FIG. 5). At this time, the ratio of the delivery speed of the feed roller 42 and the winding speed of the winder 44 can also be controlled so that the length of the modified fibroin fiber 38 does not change. The obtained modified fibroin fiber 38 is then wound by the winder 44 to obtain a wound product of the modified fibroin fiber 38.

[0214] Instead of the pair of hot rollers 58, the raw fiber 36 after contact with water 47 may be dried using drying equipment consisting of only a heat source, such as a dry heat plate 64 as shown in Figure 7(b). In this case, the length of the modified fibroin fiber can be prevented from changing by adjusting the relative speed between the delivery speed of the feed roller 42 and the winding speed of the winder 44 in the same manner as when a pair of hot rollers 58 is used as the drying equipment. In this case, the drying means is composed of the dry heat plate 64. Also, the dryer 48 is not essential.

[0215] As described above, by using the manufacturing apparatus 40, the desired modified fibroin fibers 38 can be produced automatically, continuously, and extremely easily.

[0216] Figure 7 is an explanatory diagram showing, in outline, another example of a manufacturing apparatus for producing modified fibroin fibers. Figure 7(a) shows a processing device provided in the manufacturing apparatus that performs a contacting step (primary shrinkage), and Figure 7(b) shows a drying device provided in the manufacturing apparatus that performs a drying step. The manufacturing apparatus shown in Figure 7 has a processing device 60 that performs a contacting step with raw fibers 36, and a drying device 62 that dries raw fibers 36 after the contacting step (or modified fibroin fibers 38 produced through the contacting step), and these are structured to be independent of each other.

[0217] More specifically, the processing device 60 shown in FIG. 7(a) has a structure in which a feed roller 42, a water bath 46, and a winder 44 are arranged in order from the upstream to the downstream in the running direction of the raw fiber 36. In this processing device 60, the raw fiber 36 sent out from the feed roller 42 is immersed in water 47 in the water bath 46 to cause it to shrink. The modified fibroin fiber 38 obtained is then wound up by the winder 44. At this time, for example, the winding speed of the winder 44 is set slower than the sending speed of the feed roller 42. This prevents tension from being applied to the fiber because the raw fiber 36 shrinks due to contact with the water 47 while in a relaxed state between the feed roller 42 and the winder 44. The raw fiber 36 shrinks irreversibly due to contact with the water 47 (corresponding to the "primary shrinkage" in FIG. 5).

[0218] 7(b) includes a feed roller 42, a winder 44, and a dry heat plate 64. The dry heat plate 64 is disposed between the feed roller 42 and the winder 44 such that the dry heat surface 66 is in contact with the modified fibroin fiber 38 and extends along the traveling direction of the fiber. As described above, in this drying device 62, for example, by controlling the ratio between the feed speed of the feed roller 42 and the winding speed of the winder 44, it is also possible to keep the length of the modified fibroin fiber 38 unchanged.

[0219] By using a manufacturing apparatus having such a structure, the raw fibers 36 can be shrunk by the processing device 60 to obtain the modified fibroin fibers 38, and then the modified fibroin fibers 38 can be dried in the drying device 62.

[0220] The feed roller 42 and the winder 44 may be omitted from the processing apparatus 60 shown in Fig. 7(a), and the processing apparatus may be configured with only the water bath 46. When a manufacturing apparatus having such a processing apparatus is used, for example, the modified fibroin fiber is manufactured by a so-called batch method. Also, the drying apparatus 62 shown in Fig. 7(b) is not essential.

[0221] [Shrinkage process by heat relaxation] The shrinkage step of irreversibly shrinking the raw fiber may be performed by heating and relaxing the raw fiber. The thermal relaxation of the raw fiber can be performed by heating the raw fiber and relaxing the raw fiber in the heated state to cause it to shrink. Hereinafter, in the shrinkage of the raw fiber by thermal relaxation, the step of heating the raw fiber is referred to as the "heating step", and the step of relaxing the raw fiber in the heated state to cause it to shrink is referred to as the "relaxation and shrinkage step". The heating step and the relaxation and shrinkage step can be performed, for example, by a high-temperature heating and relaxation device 140 shown in Figs. 8 and 9.

[0222] (Heating process) In the heating step, the heating temperature of raw fiber 36 is preferably equal to or higher than the softening temperature of the modified fibroin used in raw fiber 36. In this specification, the softening temperature of the modified fibroin refers to the temperature at which raw fiber 36 starts to shrink due to stress relaxation. In the thermal relaxation shrinkage at or above the softening temperature of the modified fibroin, the fiber shrinks to an extent that cannot be obtained by simply removing moisture from the fiber, and as a result, the residual stress in the fiber caused by stretching during the spinning process can be removed.

[0223] An example of the temperature corresponding to the softening temperature is 180°C. When thermal relaxation shrinkage is performed in a high temperature range of 180°C or higher, the larger the relaxation ratio or the higher the temperature, the more efficiently the residual stress in the raw fiber can be removed. Therefore, the heating temperature of the raw fiber 36 is preferably 180°C or higher, more preferably 180°C to 280°C, even more preferably 200°C to 240°C, and particularly preferably 220°C to 240°C.

[0224] The heating time in the heating step, i.e., the residence time in the high-temperature heating furnace 143, is preferably 60 seconds or less, more preferably 30 seconds or less, and even more preferably 5 seconds or less, from the viewpoint of not impairing the elongation of the fiber after the heat treatment. It is considered that the length of this heating time does not have a significant effect on the stress. Note that, if the heating temperature is 200°C and the heating time is 5 seconds or less, it is possible to prevent a decrease in the elongation of the fiber after the heat treatment.

[0225] (Relaxation and contraction process) In the relaxation contraction step, the relaxation ratio is preferably more than 1, more preferably 1.4 or more, even more preferably 1.7 or more, and particularly preferably 2 or more. The relaxation ratio is the ratio of the let-off speed to the take-up speed of the raw fiber 36, and more specifically, the ratio of the let-off speed by the let-off roller 141 to the take-up speed by the take-up roller 142.

[0226] In the heating and relaxation method using the high-temperature heating and relaxation device 140, the heating step and the relaxation and shrinkage step may be carried out separately, so long as the raw fibers 36 can be relaxed in a heated state. That is, the heating device may be an independent device separated from the relaxation device. In that case, the relaxation device is provided downstream of the heating device (downstream in the running direction of the raw fibers 36) so that the relaxation and shrinkage step is carried out after the heating step.

[0227] A heating and relaxing process may be performed on the raw fiber separately from the manufacturing process of the raw fiber. That is, a device similar to the high-temperature heating and relaxing device 140 may be provided as an independent device separate from the spinning device 25. A method may be adopted in which the separately manufactured raw fiber 36 is set on a delivery roller and delivered from there. The heating and relaxing process may be performed on one raw fiber or on multiple bundled fibers.

[0228] [Crosslinking process] A crosslinking step may be further carried out on the modified fibroin fiber having a history of irreversible shrinkage obtained as described above, or on the raw fiber before irreversible shrinkage, to chemically crosslink between polypeptide molecules in the fiber. Examples of functional groups that can be crosslinked include amino groups, carboxyl groups, thiol groups, and hydroxyl groups. For example, the amino group of a lysine side chain contained in a polypeptide can be crosslinked with a carboxyl group of a glutamic acid or aspartic acid side chain through an amide bond by dehydration condensation. Crosslinking may be carried out by carrying out a dehydration condensation reaction under vacuum heating, or may be carried out using a dehydration condensation agent such as carbodiimide.

[0229] Cross-linking between polypeptide molecules may be performed using a cross-linking agent such as carbodiimide or glutaraldehyde, or an enzyme such as transglutaminase. Carbodiimides are represented by the general formula R 1 N=C=NR 2 (However, R 1 and R 2each independently represents an organic group containing an alkyl group or a cycloalkyl group having 1 to 6 carbon atoms. Specific examples of carbodiimides include 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), N,N'-dicyclohexylcarbodiimide (DCC), 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide, diisopropylcarbodiimide (DIC), and the like. Among these, EDC and DIC are preferred because they have a high ability to form amide bonds between polypeptide molecules and are easily crosslinked.

[0230] The crosslinking treatment is preferably carried out by applying a crosslinking agent to the fibers and crosslinking the fibers by vacuum heating and drying. The crosslinking agent may be applied to the fibers in its pure form, or may be applied to the fibers after diluting the agent with a lower alcohol having 1 to 5 carbon atoms, a buffer solution, or the like to a concentration of 0.005 to 10% by mass. The crosslinking treatment is preferably carried out at a temperature of 20 to 45°C for 3 to 42 hours. The crosslinking treatment can impart even higher stress (strength) to the fibers.

[0231] [Modified fibroin fiber] The modified fibroin fiber according to the present embodiment is a modified fibroin fiber having a history of being irreversibly shrunk after spinning, and preferably contains modified fibroin and has a fiber diameter of more than 25 μm before being irreversibly shrunk. The modified fibroin fiber according to the present embodiment is obtained, for example, by the above-mentioned production method, and therefore does not substantially contain residual stress caused by drawing during the spinning process.

[0232] <Shrinkage rate> The modified fibroin fiber according to this embodiment preferably has a shrinkage percentage, as defined by the following formula (1), of 3.3% or less. Equation (1): Shrinkage rate (%) = (1 - (length of modified fibroin fiber when dried from a wet state / length of modified fibroin fiber when wet)) x 100 =(1-(Ldry / Lwet))×100

[0233] The shrinkage of a fiber due to contact with water can be evaluated, for example, using as an index the shrinkage percentage calculated by the above formula (1). The "length of the modified fibroin fiber when in a wet state" and the "length of the modified fibroin fiber when dried from a wet state" can be measured, for example, by the following method.

[0234] Several modified fibroin fibers with a length of approximately 30 cm are bundled together to obtain a fiber bundle with a fineness of 150 denier. This fiber bundle is immersed (wet) in water at 40°C for 15 minutes, and then left to dry at room temperature for 2 hours. After drying, the length of the fiber bundle is measured. The wetting and drying process is repeated at least three times, and the average length when wet is taken as the "length of the modified fibroin fiber when brought to a wet state," and the average length when dry is taken as the "length of the modified fibroin fiber when dried from a wet state."

[0235] In modified fibroin fibers, the less such shrinkage the better, particularly in products such as textiles made from modified fibroin fibers.

[0236] Fibroin fibers spun from naturally occurring fibroin typically have a shrinkage rate of 11 to 20%, but the modified fibroin fiber of the present invention has a fiber diameter of more than 25 μm before the raw fiber irreversibly shrinks, thereby making it possible to reduce the shrinkage rate due to contact with moisture, as defined by the above formula (1), to 3.3% or less.

[0237] The shrinkage percentage defined by formula (1) may be 3.2% or less, 3.1% or less, 3.0% or less, 2.9% or less, 2.8% or less, 2.7% or less, 2.6% or less, 2.5% or less, 2.4% or less, 2.3% or less, 2.2% or less, 2.1% or less, 2.0% or less, 1.5% or less, 1.0% or less, or 0.5% or less.

[0238] The modified fibroin fiber according to this embodiment can have a variety of cross-sectional shapes depending on the shape of the spinneret, but the cross-sectional shape of the modified fibroin fiber may be circular or elliptical.

[0239] The modified fibroin fiber according to this embodiment may have a matte appearance or a glossy appearance. The glossiness of the fiber appearance can be adjusted by appropriately adjusting the solvent removal rate and / or the coagulation rate in the spinning process. In this specification, the term "matte appearance" refers to an appearance with low gloss.

[0240] The modified fibroin fiber according to the present embodiment may be a modified fibroin fiber that contains modified fibroin, has a fiber diameter of more than 25 μm, and has a shrinkage rate of 3.3% or less as defined by the above formula (1). The lower limit of the fiber diameter of the modified fibroin fiber according to the present embodiment is preferably more than 25 μm, but may be 28 μm or more, 30 μm or more, 32 μm or more, 33 μm or more, more than 33 μm, 34 μm or more, 35 μm or more, 36 μm or more, 38 μm or more, 40 μm or more, 45 μm or more, 50 μm or more, 55 μm or more, or 65 μm or more.

[0241] The upper limit of the fiber diameter of the modified fibroin fiber in this embodiment is preferably 120 μm or less, and may be 115 μm or less, 110 μm or less, 105 μm or less, 100 μm or less, 95 μm or less, 90 μm or less, 85 μm or less, 80 μm or less, or 75 μm or less. The fiber diameter of the modified fibroin fiber may be greater than 25 μm to 120 μm, greater than 25 μm to 115 μm, greater than 25 μm to 110 μm, greater than 25 μm to 105 μm, greater than 25 μm to 100 μm, greater than 25 μm to 95 μm, greater than 25 μm to 90 μm, greater than 25 μm to 85 μm, 30 μm to 120 μm, 30 μm to 115 μm, 30 μm to 110 μm, or 30 μm to 105 μm. may be 30 μm to 100 μm, may be 30 μm to 95 μm, may be 30 μm to 90 μm, may be 30 μm to 85 μm, may be more than 33 μm to 120 μm, may be 34 μm or more to 120 μm, may be 35 μm to 120 μm, may be 35 μm to 115 μm, may be 35 μm to 110 μm, may be 35 μm to 105 μm, may be 35 μm to 100 μm, may be 35 μm to 95 μm, may be 35 μm to 90 μm may be 35 μm to 85 μm, may be 40 μm to 120 μm, may be 40 μm to 115 μm, may be 40 μm to 110 μm, may be 40 μm to 105 μm, may be 40 μm to 100 μm, may be 40 μm to 95 μm, may be 40 μm to 90 μm, may be 40 μm to 85 μm, may be 45 μm to 120 μm, may be 45 μm to 115 μm, may be 45 μm to 110 μm, or may be 45 μm to 105 μm. may be 45 μm to 100 μm, may be 45 μm to 95 μm, may be 45 μm to 90 μm, may be 45 μm to 85 μm, may be 48 μm to 120 μm, may be 48 μm to 115 μm, may be 48 μm to 110 μm, may be 48 μm to 105 μm, may be 48 μm to 100 μm, may be 48 μm to 95 μm, may be 48 μm to 90 μm, may be 48 μm to 85 μm, may be 50 μm to 120 μm,It may be 50 μm to 115 μm, may be 50 μm to 110 μm, may be 50 μm to 105 μm, may be 50 μm to 100 μm, may be 50 μm to 95 μm, may be 50 μm to 90 μm, may be 50 μm to 85 μm, may be 55 μm to 120 μm, may be 55 μm to 115 μm, may be 55 μm to 110 μm, may be 55 μm to 105 μm, may be 60 μm to 120 μm, may be 60 μm to 115 μm, may be 60 μm to 110 μm, may be 60 μm to 105 μm, may be 60 μm to 100 μm, 60 μm The fiber diameter may be 60 μm to 90 μm, 60 μm to 85 μm, 65 μm to 120 μm, 65 μm to 115 μm, 65 μm to 110 μm, 65 μm to 105 μm, 65 μm to 100 μm, 65 μm to 95 μm, 65 μm to 90 μm, 65 μm to 85 μm, 55 μm to 100 μm, 55 μm to 95 μm, 55 μm to 90 μm, 55 μm to 85 μm, 55 μm to 80 μm, or 60 μm to 80 μm. By making the fiber diameter more than 25 μm, shrinkage due to contact with moisture can be sufficiently reduced. By making the fiber diameter 120 μm or less, productivity can be further increased. ,

[0242] The modified fibroin fiber according to this embodiment preferably has a fiber diameter that changes little before and after the shrinkage step in which the raw fiber is irreversibly shrunk. Specifically, the modified fibroin fiber preferably has a fiber diameter that is less than ±20% of the fiber diameter of the raw fiber before it is irreversibly shrunk. It is preferable that the fiber diameter of the modified fibroin fiber is less than ±20% relative to the fiber diameter of the raw fiber, but it may be ±19% or less, may be ±18% or less, may be ±17% or less, may be ±16% or less, may be ±15% or less, may be less than ±15%, may be ±12% or less, may be ±10% or less, may be less than ±10%, may be ±5% or less, may be less than ±5%, may be ±4% or less, may be less than ±4%, may be ±3% or less, may be less than ±3%, may be ±2% or less, may be less than ±2%, may be ±1% or less, may be less than ±1%, may be ±0.9% or less, may be ±0.8% or less, may be ±0.7% or less, may be ±0.7% or less, may be ±0.6% or less, may be ±0.5% or less, may be less than ±0.5%, or may be ±0.45% or less. The above value can be calculated using the formula: (fiber diameter of modified fibroin fiber-fiber diameter of raw fiber) / fiber diameter of raw fiber x 100%.

[0243] 〔product〕 The modified fibroin fibers according to the present embodiment can be used as fibers (long fibers, short fibers, monofilaments, multifilaments, etc.) or yarns (spun yarns, twisted yarns, false twisted yarns, textured yarns, blended yarns, blended yarns, etc.) in woven fabrics (cloths), knitted fabrics, braided fabrics, nonwoven fabrics, etc., as well as in paper and cotton. They can also be used in high-strength applications such as ropes, surgical sutures, flexible fasteners for electrical components, and even bioactive materials for transplants (e.g., artificial ligaments and aortic bands). These can be produced according to known methods. EXAMPLES

[0244] The present invention will be described in more detail below with reference to examples, although the present invention is not limited to the following examples.

[0245] [Production of modified fibroin] (1) Construction of expression vector Based on the nucleotide sequence and amino acid sequence of fibroin derived from Nephila clavipes (GenBank accession number: P46804.1, GI: 1174415), modified fibroin having SEQ ID NO: 40 (hereinafter also referred to as "PRT966"), modified fibroin having SEQ ID NO: 15 (hereinafter also referred to as "PRT799"), and modified fibroin having SEQ ID NO: 37 (hereinafter also referred to as "PRT918") were designed. The amino acid sequence shown in SEQ ID NO: 40 has a sequence in which all QQ in the sequence in which the region of 20 domain sequences present in the amino acid sequence shown in SEQ ID NO: 7 is repeated twice is replaced with VF, and the remaining Q is replaced with I, in order to improve hydrophobicity, and further the amino acid sequence shown in SEQ ID NO: 11 is added to the N-terminus. In addition, the amino acid sequence shown in SEQ ID NO: 15 has an amino acid sequence in which amino acid residues have been substituted, inserted, and deleted from the amino acid sequence of fibroin derived from Nephila clavipes for the purpose of improving productivity, and further has the amino acid sequence shown in SEQ ID NO: 12 (tag sequence and hinge sequence) added to the N-terminus.

[0246] Next, nucleic acids encoding modified fibroins PRT966, PRT799, and PRT918 having the designed amino acid sequences of SEQ ID NO: 40, SEQ ID NO: 15, and SEQ ID NO: 37 were synthesized. An NdeI site was added to the 5' end of the nucleic acid, and an EcoRI site was added downstream of the termination codon. The nucleic acid was cloned into a cloning vector (pUC118). After that, the nucleic acid was excised by restriction enzyme treatment with NdeI and EcoRI, and then recombined into the protein expression vector pET-22b(+), respectively, to obtain an expression vector.

[0247] (2) Expression of engineered fibroin The expression vector obtained in (1) was used to transform E. coli BLR(DE3). The transformed E. coli was cultured in 2 mL of LB medium containing ampicillin for 15 hours. The culture was transferred to 100 mL of seed culture medium (Table 4) containing ampicillin at OD 200. 600 The culture temperature was kept at 30°C, and the OD 600 The flask culture was continued until the number of cells reached 5 (about 15 hours), and a seed culture solution was obtained.

[0248] [Table 4]

[0249] The seed culture solution was transferred to a jar fermenter containing 500 mL of production medium (Table 5) and the OD 600 The culture temperature was kept at 37°C and the pH was controlled to be constant at 6.9. The dissolved oxygen concentration in the culture was maintained at 20% of the dissolved oxygen saturation concentration.

[0250] [Table 5]

[0251] Immediately after the glucose in the production medium was completely consumed, the feed solution (glucose 455g / 1L, yeast extract 120g / 1L) was added at a rate of 1mL / min. The culture temperature was kept at 37°C and the culture was controlled to a constant pH of 6.9. The dissolved oxygen concentration in the culture was maintained at 20% of the dissolved oxygen saturation concentration, and the culture was continued for 20 hours. Then, 1M isopropyl-β-thiogalactopyranoside (IPTG) was added to the culture solution to a final concentration of 1mM to induce the expression of the modified fibroin. After 20 hours had passed after the addition of IPTG, the culture solution was centrifuged and the cells were collected. SDS-PAGE was performed using the cells prepared from the culture solution before and after the addition of IPTG, and the expression of the modified fibroin of interest was confirmed by the appearance of a band of the desired modified fibroin size depending on the addition of IPTG.

[0252] (3) Purification of modified fibroin The cells harvested 2 hours after the addition of IPTG were washed with 20 mM Tris-HCl buffer (pH 7.4). The washed cells were suspended in 20 mM Tris-HCl buffer (pH 7.4) containing approximately 1 mM PMSF, and the cells were disrupted with a high-pressure homogenizer (GEA Niro Soavi). The disrupted cells were centrifuged to obtain a precipitate. The precipitate was washed with 20 mM Tris-HCl buffer (pH 7.4) until it reached a high purity. The washed precipitate was suspended in 8 M guanidine buffer (8 M guanidine hydrochloride, 10 mM sodium dihydrogen phosphate, 20 mM NaCl, 1 mM Tris-HCl, pH 7.0) to a concentration of 100 mg / mL, and stirred with a stirrer at 60°C for 30 minutes to dissolve. After dissolution, dialysis was performed against water using a dialysis tube (Cellulose tube 36 / 32, Sanko Junyaku Co., Ltd.). The white aggregated protein obtained after dialysis was collected by centrifugation, the water was removed using a freeze-dryer, and the freeze-dried powder was collected to obtain modified fibroins (PRT966, PRT799, and PRT918).

[0253] [Production of raw fiber] (1) Preparation of dope solution As a dissolving solvent, dimethyl sulfoxide (DMSO) in which 4.0% by mass of LiCl was dissolved was prepared, and mixed with 26% by mass of the modified fibroin (PRT966) obtained in the above-mentioned modified fibroin manufacturing process, and heated with stirring in an aluminum block heater at 90°C for 1 hour to dissolve the modified fibroin. The mixture was filtered through a metal filter with 1 μm openings and degassed to prepare a dope solution. (2) Dry and wet spinning The prepared dope solution was filled in a reserve tank, and the spinning apparatus of FIG. 4 was used to discharge the dope solution from a 0.3 mm diameter monohole nozzle into a 100% by mass methanol coagulation bath using a gear pump to coagulate the solution, forming a fibrous coagulate. The fibrous coagulate was then stretched in a water washing bath. The fiber diameter was controlled by adjusting the stretch ratio conditions in the water washing bath. After washing in the water washing bath, the dope solution was dried using a dry heat plate to obtain raw fiber that would become the basis of the modified fibroin fiber of Examples 1 to 5 and Comparative Example. The obtained raw fiber was wound up with a winder. The conditions for dry and wet spinning were as follows. Extrusion nozzle diameter: 0.3mm Temperature of coagulation liquid: 5℃ Stretching ratio in water washing bath: 2.0 to 6.0 times Water cleaning bath temperature: 40℃ Drying temperature: 60℃

[0254] [Evaluation of fiber diameter of raw fiber] The diameter of the raw fiber obtained in (2) above was calculated using an optical microscope and is shown in Table 6. The measured value was the average value of the sample number n=5.

[0255] [Production of modified fibroin fibers] (1) Shrinkage process (contact process and drying process) The raw fiber obtained in (2) above was immersed in 40°C water using the spinning apparatus of FIG. 4 to shrink it and remove residual stress in the fiber resulting from the manufacturing process. The fiber was dried using a hot plate to obtain the modified fibroin fibers of Examples 1 to 5 and the Comparative Example. The modified fibroin fibers obtained were wound up on a winder. At this time, the winding speed of the winder was set slower than the delivery speed of the feed roller to prevent stress from being applied to the fiber. The fiber diameters of the modified fibroin fibers obtained in Examples 1 to 5 and the Comparative Example are shown in Table 6. (2) Evaluation of cross-sectional shape and appearance of modified fibroin fibers Figure 10 shows a scanning electron microscope (SEM) image of the cross-sectional shape of the modified fibroin fiber obtained in (1). It can be observed that the cross-sectional shape of the fiber is circular. When visually evaluated, the modified fibroin fiber obtained was found to have a matte appearance compared to natural silk fibers.

[0256] (3) Contractility evaluation of modified fibroin fibers The modified fibroin fibers obtained in (1) were bundled together to a length of approximately 30 cm to obtain a fiber bundle with a fineness of 150 denier. This fiber bundle was immersed (wet) in water at 40°C for 15 minutes and then dried at room temperature for 2 hours. After drying, the length of the fiber bundle was measured. The wetting and drying were repeated at least three times, and the average length when wet was taken as the length of the modified fibroin fiber when brought to a wet state (Lwet), and the average length when dry was taken as the length of the modified fibroin fiber when dried from a wet state (Ldry). The shrinkage rate was calculated according to the following formula. The measured value was the average value of the number of samples n=3. Formula: Shrinkage rate (%) = (1-(Ldry / Lwet)) x 100

[0257] The shrinkage percentage of the modified fibroin fiber for each fiber diameter is shown in Table 6. For reference, the relative values ​​when the shrinkage percentage of the modified fibroin fiber of Comparative Example 1 was set at 100 are also shown. [Table 6]

[0258] As shown in Table 6, the modified fibroin fibers with a fiber diameter of more than 25 μm (Examples 1 to 5) had a lower shrinkage rate and reduced shrinkage in water compared to the modified fibroin fibers with a fiber diameter of less than 25 μm (Comparative Example). Furthermore, the modified fibroin fibers with a fiber diameter of 61 μm to 81 μm (Examples 2 and 3) had the greatest effect of reducing shrinkage in water. Furthermore, the fiber diameter of the modified fibroin fibers relative to the fiber diameter of the raw fiber was a maximum of 0.41% and a minimum of -0.02%, indicating extremely good dimensional stability.

[0259] Reference Example 1: Flammability test of modified fibroin The freeze-dried powder of modified fibroin (PRT799) was added to a dimethyl sulfoxide solution of lithium chloride (concentration: 4.0% by mass) to a concentration of 24% by mass, and dissolved by mixing for 3 hours using a shaker. Insoluble matter and bubbles were then removed to obtain a modified fibroin solution (spinning stock solution).

[0260] The obtained spinning solution was heated to 90°C, filtered through a metal filter with a mesh size of 5 μm, and then allowed to stand in a 30 mL stainless steel syringe to degas it, after which it was discharged from a solid nozzle with a needle diameter of 0.2 mm into a 100% by mass methanol coagulation bath. The discharge temperature was 90°C. After coagulation, the obtained raw yarn was wound and naturally dried to obtain a modified fibroin fiber (raw fiber).

[0261] A knitted fabric (thickness: 180 denier, gauge number: 18) was produced by circular knitting using a circular knitting machine using a twisted yarn obtained by twisting the raw fibers together. 20 g of the knitted fabric was cut out and used as a test piece.

[0262] The flammability test was conducted in accordance with the "Test method for powdered or low melting point synthetic resins" described in "Fire Safety Hazard No. 50 (dated May 31, 1995)". The test was conducted under conditions of a temperature of 22°C, relative humidity of 45%, and atmospheric pressure of 1021hPa. The measurement results (oxygen concentration (%), flammability rate (%), converted flammability rate (%)) are shown in Table 7. [Table 7]

[0263] As a result of the flammability test, the limiting oxygen index (LOI) value of the fabric knitted with the modified fibroin (PRT799) fiber was 27.2. Generally, an LOI value of 26 or more is known to be flame retardant. This shows that the modified fibroin has excellent flame retardancy.

[0264] Reference Example 2: Evaluation of hygroscopic heat generation of modified fibroin The freeze-dried powder of modified fibroin was added to a dimethyl sulfoxide solution of lithium chloride (concentration: 4.0% by mass) to a concentration of 24% by mass, and dissolved by mixing for 3 hours using a shaker. Insoluble matter and bubbles were then removed to obtain a modified fibroin solution (spinning stock solution).

[0265] The obtained spinning solution was heated to 60°C, filtered through a metal filter with a mesh size of 5 μm, and then allowed to stand in a 30 mL stainless steel syringe to degas it, after which it was discharged from a solid nozzle with a needle diameter of 0.2 mm into a 100% by mass methanol coagulation bath. The discharge temperature was 60°C. After coagulation, the obtained raw yarn was wound and naturally dried to obtain a modified fibroin fiber (raw fiber).

[0266] For comparison, commercially available wool fibers, cotton fibers, Tencel fibers, rayon fibers and polyester fibers were prepared as raw fibers.

[0267] Each raw fiber was used to produce a knitted fabric by flat knitting using a flat knitting machine. The thickness and gauge number of the knitted fabrics using PRT918 fiber or PRT799 fiber are shown in Table 8. For the knitted fabrics using other raw fibers, the thickness and gauge number were adjusted so that the cover factor was approximately the same as that of the knitted fabric of the modified fibroin fiber. Specifically, the thickness and gauge number are as follows. [Table 8]

[0268] Two pieces of knitted fabric cut to 10cm x 10cm were put together and sewn together at all four sides to make a test piece (sample). The test piece was left in a low humidity environment (temperature 20±2°C, relative humidity 40±5%) for more than 4 hours, then transferred to a high humidity environment (temperature 20±2°C, relative humidity 90±5%), and the temperature was measured at 1-minute intervals for 30 minutes using a temperature sensor attached to the center inside the test piece.

[0269] From the measurement results, the maximum moisture absorption heat generation was calculated according to the following formula A. Formula A: Maximum moisture absorption heat release rate = {(Maximum temperature of the sample when it is placed in a low humidity environment until the temperature of the sample reaches equilibrium and then transferred to a high humidity environment) - (Temperature of the sample when it is placed in a low humidity environment until the temperature of the sample reaches equilibrium and then transferred to a high humidity environment)} (°C) / Sample weight (g)

[0270] Figure 11 is a graph showing an example of the results of a moisture absorption heat generation test. The horizontal axis of the graph indicates the time (minutes) that the sample was left in the high humidity environment, with the point at which the sample was moved from a low humidity environment to a high humidity environment being set as 0. The vertical axis of the graph indicates the temperature (sample temperature) measured by the temperature sensor. In the graph shown in Figure 11, the point indicated by M corresponds to the maximum value of the sample temperature.

[0271] The calculation results of the maximum moisture absorption heat generation rate of each knitted fabric are shown in Table 9. [Table 9]

[0272] As shown in Table 9, the modified fibroins (PRT918 and PRT799) have a higher maximum hygroscopic heat generation value and are superior in hygroscopic heat generation properties compared to existing materials.

[0273] Reference Example 3: Evaluation of heat retention of modified fibroin The freeze-dried powder of modified fibroin was added to a dimethyl sulfoxide solution of lithium chloride (concentration: 4.0% by mass) to a concentration of 24% by mass, and dissolved by mixing for 3 hours using a shaker. Insoluble matter and bubbles were then removed to obtain a modified fibroin solution (spinning stock solution).

[0274] The obtained spinning solution was heated to 60°C, filtered through a metal filter with a mesh size of 5 μm, and then allowed to stand in a 30 mL stainless steel syringe to degas it, after which it was discharged from a solid nozzle with a needle diameter of 0.2 mm into a 100% by mass methanol coagulation bath. The discharge temperature was 60°C. After coagulation, the obtained raw yarn was wound and naturally dried to obtain a modified fibroin fiber (raw fiber).

[0275] For comparison, commercially available wool fibers, silk fibers, cotton fibers, rayon fibers and polyester fibers were prepared as raw fibers.

[0276] Each raw fiber was used to produce a knitted fabric by flat knitting using a flat knitting machine. The yarn count, number of twists, gauge number, and basis weight of the knitted fabrics using PRT966 fiber or PRT799 fiber are as shown in Table 10. The knitted fabrics using other raw fibers were adjusted to have almost the same cover factor as the knitted fabric of the modified fibroin fiber. Specifically, it is as follows. [Table 10]

[0277] The heat retention was evaluated using a KES-F7 Thermo Lab II tester manufactured by Kato Tech Co., Ltd., using the dry contact method (a method that assumes direct contact between the skin and clothing in a dry state). A piece of knitted fabric cut into a rectangle of 20 cm x 20 cm was used as the test piece (sample). The test piece was set on a hot plate set at a constant temperature (30°C), and the amount of heat (a) dissipated through the test piece was determined under the condition of a wind speed of 30 cm / sec in a wind tunnel. The amount of heat (b) dissipated under the same conditions as above was determined without the test piece being set, and the heat retention rate (%) was calculated according to the following formula B. Formula B: Heat retention rate (%)=(1-a / b)×100

[0278] From the measurement results, the heat retention index was calculated according to the following formula C. Formula C: Heat retention index = Heat retention rate (%) / sample weight (g / m 2 )

[0279] The results of calculating the heat retention index are shown in Table 11. The higher the heat retention index, the better the material's heat retention properties can be evaluated.

[0280] [Table 11]

[0281] As shown in Table 11, the modified fibroins (PRT966 and PRT799) have a higher thermal insulation index and are superior in thermal insulation properties compared to existing materials.

[0282] As shown in Reference Examples 1 to 3, when the modified fibroin is modified spider silk fibroin, the fiber can have better heat retention, moisture absorption and heat generation properties, and / or flame retardancy. By using modified spider silk fibroin to produce the fiber of the present invention, it is possible to obtain a fiber that has better heat retention, moisture absorption and heat generation properties, and / or flame retardancy and a reduced shrinkage rate due to moisture. [Explanation of symbols]

[0283] Reference Signs List 1...extrusion device, 2...undrawn yarn manufacturing device, 3...wet heat drawing device, 4...drying device, 6...dope solution, 10...spinning device, 20...coagulation bath, 21...drawing bath, 25...spinning device, 36...raw fiber, 38...modified fibroin fiber, 40...manufacturing device, 42...feed roller, 44...winder, 46...water bath, 48...dryer, 54...heater, 56...tension roller, 58...hot roller, 60...processing device, 62...drying device, 64...dry heat plate, 140...relaxation and contraction means (heating means), 141...sending means, 142...winding means, 146...speed adjustment means, 147...temperature adjustment means.

Claims

1. A modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning, the modified fibroin fiber containing modified fibroin, and the fiber diameter of the raw fiber before being irreversibly shrunk is 55 μm or more and 100 μm or less.

2. The modified fibroin fiber according to claim 1, wherein the shrinkage history is a shrinkage history in which the raw fiber is irreversibly shrunk by contacting it with water or a shrinkage history in which the raw fiber is irreversibly shrunk by heating and relaxing it.

3. 3. The modified fibroin fiber of claim 1 or 2, which is substantially free of residual stress caused by drawing during the spinning process.

4. The modified fibroin fiber according to any one of claims 1 to 3, having a shrinkage rate defined by the following formula (1) of 3.3% or less: Equation (1): Shrinkage rate (%)=(1-(length of modified fibroin fiber when dried from a wet state / length of modified fibroin fiber when wet))×100

5. The modified fibroin fiber of any one of claims 1 to 4, wherein the modified fibroin is modified spider silk fibroin.

6. The modified fibroin fiber of any one of claims 1 to 5, wherein the modified fibroin is a hydrophobic modified spider silk fibroin.

7. The modified fibroin fiber of any one of claims 1 to 6, having a cross-sectional shape that is circular or elliptical.

8. An article of manufacture comprising the modified fibroin fiber of any one of claims 1 to 7.

9. 9. The article of manufacture of claim 8, wherein the article is selected from the group consisting of fiber, yarn, fabric, knit, braid, nonwoven, paper, and cotton.

10. A shrinking step is provided for irreversibly shrinking the raw fiber. The raw fiber comprises modified fibroin, A method for producing a modified fibroin fiber, wherein the raw fiber has a fiber diameter of 55 μm or more and 100 μm or less before the shrinking step.

11. The method according to claim 10, wherein in the shrinking step, the raw fiber is irreversibly shrunk by contacting the raw fiber with water or by heating and relaxing the raw fiber.

12. A modified fibroin fiber comprising a modified fibroin, having a fiber diameter of 55 μm or more and 100 μm or less, and having a shrinkage rate of 3.3% or less as defined by the following formula (1). Equation (1): Shrinkage rate (%)=(1-(length of modified fibroin fiber when dried from a wet state / length of modified fibroin fiber when wet))×100

13. The modified fibroin fiber of claim 12, wherein the modified fibroin fiber has a shrinkage history in which the fiber was irreversibly shrunk after spinning.

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