Spinach plant

Abstract

To provide a spinach variety that shows resistance to downy mildew.SOLUTION: The inventive spinach plants include a spinach plant identified with accession number FERM ABP-22406, accession number FERM ABP-22407, accession number FERM ABP-22408, accession number FERM ABP-22409, accession number FERM ABP-22410, accession number FERM ABP-22411, or accession number FERM ABP-22412 or a progeny line thereof.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to spinach plants. [Background technology]

[0002] In spinach cultivation, damage caused by the downy mildew pathogen is a serious problem worldwide. Plants infected with the downy mildew pathogen (downy mildew fungus) experience reduced growth due to the withering and dying of leaves, resulting in reduced yields.

[0003] For this reason, attempts have been made to develop varieties that are resistant to downy mildew by utilizing resistance loci against downy mildew. However, downy mildew fungi that can infect spinach plants containing these resistance loci have appeared, causing problems (Non-Patent Document 1). [Prior art documents] [Non-patent literature]

[0004] [Non-Patent Document 1] Shyam L. Kandel et.al., “Spinach Downy Mildew: Advances in Our Understanding of the Disease Cycle and Prospects for Disease Management”, Plant Disease, 2019, vol 103, pages 791-803 Summary of the Invention [Problem to be solved by the invention]

[0005] Therefore, an object of the present invention is to provide a downy mildew-resistant spinach plant or seeds thereof. [Means for solving the problem]

[0006] In order to achieve the above object, the seeds of the spinach plant of the present invention are Entrust Number FERM B P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB It has been deposited under the number P-22412.

[0007] The spinach plant of the present invention is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB It is grown from seeds deposited under P-22412.

[0008] The method for producing a spinach plant of the present invention includes a self-pollination step of self-pollinating the spinach plant of the present invention.

[0009] The method for producing a spinach plant of the present invention includes a hybridization step of hybridizing the spinach plant of the present invention with another spinach plant. Effect of the Invention

[0010] According to the present invention, it is possible to provide a downy mildew-resistant spinach plant or a seed thereof. [Brief description of the drawings]

[0011]

Figure 1

Figure 2

Figure 3

Figure 4

[0012] <Spinach plant> The spinach plant of the present invention is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB The present invention also includes the spinach plant identified (deposited) under the reference number P-22412 (hereinafter collectively referred to as the "deposited line") or its progeny. Entrust Number FER MB P-22406, receiving Entrust Number FER M B P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MBThe present invention is characterized in that it comprises a spinach plant identified as P-22412 or a progeny thereof, and other configurations and conditions are not particularly limited.

[0013] As mentioned above, race differentiation of the downy mildew pathogen is intense, and new races are emerging at an accelerating rate. For this reason, spinach plants that are completely resistant to each race have high commercial value. On the other hand, because the speed at which new races emerge is accelerating, it is becoming more difficult to breed spinach plants that are completely resistant to each race. In addition, the International Working Group of Peronospora (IWGP) is breaking through the conventional downy mildew-resistant spinach varieties and certifying new races of downy mildew pathogen strains that may become a threat in the future. The spinach plant of the present invention is resistant to some or all of the downy mildew pathogen strains (UA1014, UA201621A, UA201550, UA201707A, UA201720B, isolate 195, isolate 254) that may be certified as new races after Pfs18 in the future. Therefore, the spinach plant of the present invention is capable of conferring resistance to new races that may be identified in the future, and is therefore expected to be extremely useful in breeding and to be of high commercial value.

[0014] Hereinafter, unless otherwise specified, the "deposited strain" refers to Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, and / or Entrust Number FER MB "Spinach plant" refers to the spinach plant identified (deposited) in US Pat. No. 4,993,333 and may be read as such.

[0015] In the present invention, the "spinach plant" refers to a plant belonging to the Amaranthaceae family ( Amaranthaceae ) Chenopodiaceae ( Chenopodiaceae ) Spinach ( Spinacia Spinach species (L.) S. oleracea The spinach plant may be, for example, a hybrid with a closely related species or a wild species.

[0016] In the present invention, a "cultivated spinach plant", a "cultivated spinach variety", or a "cultivated spinach" refers to a spinach plant or a variety, breeding line, or cultivar thereof that is cultivated by humans and is agronomically excellent. A "cultivated spinach plant", a "cultivated spinach variety", or a "cultivated spinach" may be a hybrid thereof, or a hybrid with a closely related species or a wild species.

[0017] In the present invention, the term "plant" may mean either an individual plant indicating the whole plant or a part of the individual plant (a part of a plant). The "plant" may be, for example, a plant cell, a plant protoplast, a plant cell tissue culture capable of regenerating a plant body, a plant callus, a plant clump, a plant cell isolated from a plant or a part of a plant, a leaf, a pollen, an embryo, a cotyledon, an hypocotyl, a root, a root tip (root tip), an anther, a pistil, a flower, an ovary, an ovule, a seed, a fruit, a stem, a seedling, or the like. The part of the individual plant may be, for example, an organ, a tissue, a cell, a vegetative propagule, or the like, and may be any of them. The organ may be, for example, a petal, a corolla, a flower, a leaf, a seed, a fruit, a stem, a root, or the like. The tissue may be, for example, a part of the organ. Specific examples of the part of the plant individual include microspores, flowers, flower buds, pistils, anthers, pollen, ovaries, embryos, ovules, hypocotyls, embryo sacs, egg cells, cuttings, roots, root tips, stems, stalks, leaves, petioles, leaf pith, cotyledons, cells, meristematic cells, protoplasts, and seeds. The pollen may be mature or immature pollen. The part of the plant individual may be derived from any growth stage of a plant, for example, before rooting, after rooting, seedlings, cuttings, mature individuals, and the like. The part of the plant individual may be, for example, one type of organ, tissue, and / or cell, or two or more types of organs, tissues, and / or cells.

[0018] In the present invention, "downy mildew" refers to a disease caused by a filamentous fungus. The pathogenic fungus of the downy mildew is, for example, Peronospora efusa ( Peronospora effusa " Peronospora farinosa "). The race of the downy mildew pathogen (Peronospora farinosa race (Pfs)) is not particularly limited and may be a known race described below or a new race. The "race" refers to a bacterial lineage with different pathogenicity, more specifically, a lineage that exhibits different pathogenicity against varieties with different resistance genes or resistance gene loci.

[0019] In the present invention, "downy mildew resistance" is also referred to as, for example, "downy mildew resistance." The resistance means, for example, the ability to inhibit or suppress the occurrence and progression of disease caused by infection with a downy mildew pathogen, and specifically may mean, for example, no occurrence of disease, halting of the progression of an established disease, and suppression (also referred to as "inhibition") of the progression of an established disease.

[0020] In the present invention, the "downy mildew resistance" can be evaluated in accordance with the measurement method of trait number 18 described below. In the present invention, a spinach plant exhibiting the "downy mildew resistance" may be resistant to any one or more races, and may be resistant to multiple races.

[0021] <Downy mildew spinach plant> The present invention provides mildew resistant spinach plants that are resistant to several races of mildew.

[0022] The first mildew-resistant spinach plant of the present invention has, for example, the following trait (1). (1) Peronospora efusa ( Peronospora effusa ) races Pfs1 to 17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B.

[0023] First mildew-resistant spinach plants of the present invention include, for example, TK03, TK04, TK05, TK06, and TK07.

[0024] Furthermore, the second mildew-resistant spinach plant of the present invention has, for example, the following trait (2). (2) Peronospora efusa ( Peronospora effusa ) races Pfs1-3, 5-15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B.

[0025] The second mildew-resistant spinach plant of the present invention may have, for example, the following trait (2'). (2') Peronospora efusa ( Peronospora effusa ) races Pfs1 to 3, 5 to 15, and 17, as well as UA1014, UA201550, UA201707A, and UA201720B, and are susceptible (susceptible) to Pfs4, Pfs16 and / or UA201621A.

[0026] The second mildew-resistant spinach plant of the present invention may be, for example, TK01. The susceptibility may also mean not exhibiting the resistance. In the trait (2'), the second mildew-resistant spinach plant may be susceptible to any one or more races of Pfs4, Pfs16, and UA201621A, or may be susceptible to all races.

[0027] Furthermore, the third mildew-resistant spinach plant of the present invention has, for example, the following trait (3). (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B.

[0028] The third mildew-resistant spinach plant of the present invention may have, for example, the following trait (3'). (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B, and susceptible to Pfs2 and / or Pfs3.

[0029] The third mildew-resistant spinach plant of the present invention may be, for example, TK02. In the trait (3'), the second mildew-resistant spinach plant may be susceptible to either one of the races of Pfs2 and Pfs3, or may be susceptible to both races.

[0030] In the first to third downy mildew-resistant spinach plants, the downy mildew resistance can be transferred (added or imparted) to a progeny line by crossing. Therefore, in the first to third downy mildew-resistant spinach plants, it is presumed that the downy mildew resistance is imparted by a downy mildew resistance gene locus. Therefore, the downy mildew-resistant spinach plant of the present invention may be a progeny line of the first to third downy mildew-resistant spinach plants. It is preferable that the progeny line exhibits resistance to the same race as the corresponding downy mildew-resistant spinach plant. In addition, the progeny line may, for example, exhibit susceptibility to the same race as the corresponding downy mildew-resistant spinach plant.

[0031] <Deposited strain> The spinach plant of the present invention is, by way of example, Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER M B P-22411, or Entrust Number FER MB The spinach plant identified by the deposited serial number P-22412 or its progeny is included. Entrust Number FER M B P-Uke Entrust Number FER MB P-22406, receiving Entrust Number FERMB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, and Entrust Number FER MB P-22412 are also referred to as spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06 and TK07, respectively. The deposit information for each cultivar is shown below.

[0032] (TK01) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22406 Identification mark: Takii13 Received: December 28, 2020

[0033] (TK02) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22407 Identification mark: Takii14 Received: December 28, 2020

[0034] (TK03) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22408 Identification mark: Takii15 Received: December 28, 2020

[0035] (TK04) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22409 Identification mark: Takii16 Received: December 28, 2020

[0036] (TK05) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22410 Identification mark: Takii17 Received: December 28, 2020

[0037] (TK06) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22411 Identification mark: Takii18 Received: December 28, 2020

[0038] (TK07) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Receiving Entrust Number:FER MB P-22412 Identification mark: Takii19 Received: December 28, 2020

[0039] The deposited line exhibits, for example, the morphological and physiological characteristics described in Table 1 below. In Table 1 below, the morphological and physiological characteristics are based on a trial production in Japan in 2019. In Table 1 below, the morphological and physiological characteristics are evaluated based on the spinach species evaluation criteria (issued in March 2017, http: / / www.hinshu2.maff.go.jp / info / sinsakijun / kijun / 1605.pdf) issued by the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF). The morphological and physiological characteristics can be seen in Figures 1 to 4. In addition, the evaluation of the morphological and physiological characteristics may refer to the DUS test guidelines in UPOV. As the DUS test guidelines, for example, the guidelines issued on June 14, 2019 (https: / / www.upov.int / edocs / tgdocs / en / tg055.pdf) can be seen.

[0040] [Table 1]

[0041] (Trait number 2) The term "cotyledon length" refers to the length (mm) of a fully expanded cotyledon. The "cotyledon length" can be evaluated based on the following criteria: class 3 (short), class 5 (medium), and class 7 (long).

[0042] (Trait number 3) The "green shade of the leaf blade" refers to the green shade of the surface of the leaf blade of the seventh to tenth true leaves of a plant that has fully grown before bolting, and can be evaluated by visual observation. The "green shade of the leaf blade" can be evaluated based on the following criteria: grade 1 (very light), grade 3 (light, standard variety: Jiromaru), grade 5 (medium, standard variety: Ucheng), grade 7 (dark, standard variety: King of Denmark), and grade 9 (very dark).

[0043] (Trait number 4) The "intensity of unevenness of the leaf blade surface" refers to the intensity of unevenness of the leaf blade surface, and can be evaluated by visual observation. The "intensity of unevenness of the leaf blade surface" can be evaluated based on the following criteria: grade 1 (none or very weak), grade 3 (weak), grade 5 (medium, standard variety: Nobel), grade 7 (strong, standard variety: Yugiri), and grade 9 (very strong).

[0044] (Trait number 5) The "strength of the incision in the leaf blade" refers to the strength of the incision in the leaf blade, and can be evaluated by visual observation (see FIG. 1). The "strength of the incision in the leaf blade" can be evaluated based on the following criteria: grade 1 (none or very weak), grade 3 (weak), grade 5 (medium), and grade 7 (strong).

[0045] (Trait number 6) The "petiole orientation" refers to the orientation of the petiole, and can be evaluated by visual observation (see FIG. 2). The "petiole orientation" can be evaluated based on the following criteria: class 1 (upright), class 3 (diagonally upward), and class 5 (horizontal).

[0046] (Trait number 7) The "petiole length" means the length of the petiole (cm). The "petiole length" can be evaluated based on class 3 (short, standard variety: Yugiri), class 5 (medium, standard variety: Toyoba), and class 7 (long, standard variety: Yucheng).

[0047] (Trait number 8) The "orientation of the leaf blade" refers to the direction in which the leaf blade develops, and can be evaluated by visual observation (see FIG. 3). The "orientation of the leaf blade" can be evaluated based on the following criteria: Class 1 (upright), Class 3 (upward), Class 5 (horizontal), and Class 7 (downward).

[0048] (Trait number 9) The "shape of the leaf blade" refers to the shape of the leaf blade excluding the basal lobe, and can be evaluated by visual observation. The "shape of the leaf blade" can be evaluated based on the following criteria: Class 1 (triangle), Class 2 (oval), Class 3 (broad oval), Class 4 (ellipse), Class 5 (broad ellipse), and Class 6 (circle).

[0049] (Trait number 10) The "curvature of the edge of the leaf blade" refers to the direction of the curvature of the edge of the leaf blade, and can be evaluated by visual observation. The "curvature of the edge of the leaf blade" can be evaluated based on the standards of Class 1 (inward curvature), Class 2 (flat), and Class 3 (recurvature).

[0050] (Trait number 11) The "shape of the tip of the leaf blade" refers to the shape of the tip of the leaf blade, and can be evaluated by visual observation. The "shape of the tip of the leaf blade" can be evaluated based on the following criteria: Grade 1 (acute), Grade 2 (obtuse), and Grade 3 (circular).

[0051] (Trait number 12) The "shape of the longitudinal section of the leaf blade" refers to the shape of the curvature of the longitudinal section of the leaf blade, and can be evaluated by visual observation. The "shape of the longitudinal section of the leaf blade" can be evaluated based on the standards of class 1 (inwardly curved), class 2 (flat), and class 3 (recurved).

[0052] (Trait number 13) The "occurrence rate of monoecious plants" refers to the percentage (%) of individuals that bear both male and female flowers. The "occurrence rate of monoecious plants" can be evaluated with reference to Table 2 below, using the following criteria: Class 1 (none or very low), Class 3 (low), Class 5 (medium), Class 7 (high), and Class 9 (very high).

[0053] [Table 2]

[0054] (Trait number 14) The "female plant occurrence rate" refers to the rate (%) of individuals that bear only female flowers. The "female plant occurrence rate" can be evaluated with reference to Table 2, using the following criteria: Class 1 (none or very low), Class 3 (low), Class 5 (medium), Class 7 (high), and Class 9 (very high).

[0055] (Trait number 15) The "male plant occurrence rate" refers to the rate (%) of individuals that bear only male flowers. The "male plant occurrence rate" can be evaluated with reference to Table 2, using the following criteria: Class 1 (none or very low), Class 3 (low), Class 5 (medium), Class 7 (high), and Class 9 (very high).

[0056] (Trait number 16) The "bolting stage" refers to the early or late time of bolting (the day when 15% of plants bolt in spring-sown cultivation) and can be evaluated by visual observation. The bolting stage of a plant refers to the time when the central flower stalk extends from the internode and appears. The "bolting stage" can be evaluated based on class 1 (very early), class 3 (early, standard variety: Ucheng), class 5 (medium, standard variety: Toyoba), class 7 (late, standard variety: Nobel), and class 9 (very late).

[0057] (Trait number 17) The "presence or absence of thorns in the harvested seeds" refers to the presence or absence of thorn-like protrusions on the surface of the harvested seeds, and can be evaluated by visual observation (see FIG. 4). The "presence or absence of thorns in the harvested seeds" can be evaluated based on the criteria of grade 1 (absent) and grade 9 (present).

[0058] (Trait number 18) The "mildew resistance" means the presence or absence of resistance to mildew fungi (race (Pfs) 1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:7, Pfs:8, Pfs:10, etc.) in an inoculation test, and can be evaluated by visual observation. The "mildew resistance" can be evaluated based on class 1 (absent) and class 9 (present).

[0059] The mildew resistance can be confirmed by the following test method. (Testing method for mildew resistance) ·Maintaining the bacterial system Storage environment: Plant bodies on which conidia of the downy mildew fungus have formed should be frozen and stored at -20°C and used within one year. · Test execution Plant growth stage: First cotyledon / true leaf stage, 11 days after sowing Temperature: Daytime temperature 15℃, night temperature 12℃ Light: 15 hours day length after germination Cultivation method: Host and test plants are grown in a greenhouse in containers filled with potting soil. Inoculation method: Leaves on which conidia are formed are taken from the host plant 7 days after inoculation, and the resulting conidia suspension is thoroughly washed with sterilized water (maximum 150 ml / 224 plants). The resulting conidia suspension is filtered through gauze and then sprayed on the plant body so that no conidia drip from the leaves. 150 ml of suspension is sufficient to treat 3 x 224 plants. The spore concentration of the suspension to be sprayed is 20,000 to 100,000 conidia / ml (water). The suspension should be used immediately after preparation. Note: Spinach downy mildew is an anemophilous disease, so test plants with conidia should be kept isolated to prevent cross-infection. A resistant control cultivar should always be used with each propagation population and each test to ensure race specificity. Light and humidity are important during seedling and incubation. Humidity of approximately 80-90% RH (relative humidity) is optimal for plant and fungal growth. Strong light also inhibits spore germination and infection. Tests should be conducted in winter, away from direct sunlight. After inoculation, the plants should be kept covered with plastic for three days, after which the plastic cover should be opened slightly during the day. Duration of the trial Reproduction: Spores 7 days after inoculation From seed to inoculation: 11 days From vaccination to investigation: 10 days Number of specimens: 20 or 40 specimens Assessment of infection: Resistance is usually seen as necrotic lesions caused by infection, and although there is a variation in the degree of conidial formation in susceptible plants, sporulation begins on the underside of leaves where it is more humid, and is visible as grey spots. -Differential varieties for identifying races Mildew races Pfs1-17 and UA1014, UA201621A, UA201550, UA201707A, and UA201720B are defined based on the differential cultivars (Viroflay, Resistoflay, Califlay, Clermont, Campania, Boeing, Lazio, Pigeon, Caladonia, Meerkat, and Hydrus) in Tables 3A and B below. The differential cultivars in Tables 3A and B below and plant material on which conidia of Pfs1-17 are formed and stored at -20°C are available from the following: (Source of identification varieties and plant materials (Pfs1-17)) Naktuinbouw PO Box 40 NL-2370 AA Roelofarendsveen Netherlands www.naktuinbouw.com In addition, UA1014, UA201621A, UA201550, UA201707A, and UA201720B are races newly reported in the International Working Group on Peronospora in spinach (IWGP) and are described in Reference 1 below. Therefore, UA1014, UA201621A, UA201550, UA201707A, and UA201720B are available by referring to the contents of Reference 1 below. Reference 1: Chunda Feng et.al., “New Races and Novel Strains of the Spinach Downy Mildew Pathogen Peronospora effuse”, 2018, Plant Disease, vol. 102, No. 4, pages 613-618

[0060] [Table 3A]

[0061] [Table 3B]

[0062] In the present invention, a plant having "essentially all physiological and morphological characteristics of the deposited line" means a plant having the major traits of the deposited line when grown in the same environment. The major traits for each of the deposited lines are shown below. The plant having "essentially all physiological and morphological characteristics of the deposited line" may be a plant having the same traits as the corresponding deposited line (derived deposited line) except for, for example, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait, i.e., 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait may be a trait different from the corresponding deposited line (derived deposited line). The "trait different from the deposited line" may be a major trait of the deposited line or a trait other than the major trait of the deposited line, but a trait other than the major trait of the deposited line is preferable. The "traits different from those of the deposited line" can be implemented, for example, by introducing the traits and / or genes described below. In a plant having "essentially all physiological and morphological characteristics of the deposited line", all of the traits of trait numbers 2 to 18 may be the same as those of the deposited line.

[0063] <Progeny lineage> The spinach plant of the present invention may be a progeny of the deposited line. The progeny may be an individual plant of the progeny, a part of an individual plant of the progeny, or a seed of the progeny.

[0064] In the present invention, a "progeny line" or a "progeny spinach plant" (hereinafter collectively referred to as "progeny line") is a plant obtained from a spinach plant of a deposited line or a progeny line thereof. In the present invention, the progeny line may be a plant obtained by crossing the deposited line with another deposited line or another spinach plant, or by crossing the deposited line with a wild spinach plant. In addition, the progeny line may be obtained, obtainable, or derived directly or indirectly by selfing and / or cross-pollinating the deposited line or its progeny line, or may be derived from a parent line obtained from the deposited line using traditional breeding methods such as selfing and / or cross-pollination. Examples of the progeny line include a selfed progeny line, a first generation hybrid F1 (first generation hybrid line, F1 hybrid), a backcross progeny line, etc. In obtaining the progeny line, the deposited line may be used as a female parent, a male parent, or both parents.

[0065] In the present invention, "crossing" refers to the crossing of two parental lines. The crossing may be "cross-pollination" or "self-pollination". The cross-pollination refers to fertilization by the union of two gametes originating from different plants. The self-pollination refers to the transfer of pollen from anthers to the stigma of the same plant. The self-pollination may also be referred to as, for example, self-fertilization. The crossing may include backcrossing, which is one of the traditional breeding methods.

[0066] The "backcrossing" is one of the traditional breeding techniques, in which a breeder repeatedly backcrosses a progeny line of a hybrid to one of the parent lines to introduce a trait into a plant or variety. The plant containing the trait to be introduced can be called, for example, a donor plant. The plant into which the trait is introduced can be called, for example, a recurrent parent. The backcrossing can be performed by crossing a donor plant with a recurrent parent, thereby obtaining a first generation hybrid F1 (first generation hybrid line, F1 hybrid). The progeny line having the trait is then crossed with the recurrent parent. Then, by backcrossing and / or selfing for several generations, the trait of the donor plant can be introduced into the recurrent parent.

[0067] In the present invention, the progeny line may be regenerated from a cell culture or tissue culture, a protoplast or a part of a plant individual derived from the deposited line, or may be obtained by self-pollination of the deposited line, or may be obtained by producing seeds from a plant individual of the deposited line.

[0068] In the present invention, the term "regeneration" refers to the generation or vegetative propagation of plants from cell culture, tissue culture or protoplasts.

[0069] The "tissue culture" or "cell culture" may be a composition containing isolated cells of the same or different types, or may be a collection of cells organized into a plant part. Methods for tissue culture of various tissues of spinach plants and for regenerating plants from said tissue cultures are well known, see, for example, Reference 2 below. Reference 2: Jameel M. Al-Khayri et.al., “Spinach Tissue Culture Improved with Coconut Water”, HORTSCIENCE, 1992, vol. 27, No. 4, pages 357-358

[0070] The progeny line preferably has downy mildew resistance. In this case, the progeny line is resistant to at least one race selected from the group consisting of downy mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, and is preferably resistant to 2, 3, 4, 5, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22. The progeny line preferably has the same or similar downy mildew resistance as the deposited line from which it is derived. The similar mildew resistance means, for example, that the progeny line differs from the accession line from which it was derived in being resistant to mildew by one, two, three or four races of spinach plants.

[0071] As a specific example, when the progeny line is the first downy mildew-resistant spinach plant, the progeny line preferably has the trait (1) described above. When the progeny line is the second downy mildew-resistant spinach plant, the progeny line preferably has the trait (2) or (2') described above. When the progeny line is the third downy mildew-resistant spinach plant, the progeny line preferably has the trait (3) or (3') described above.

[0072] The progeny line may have a desired trait. The progeny line may have "essentially all physiological and morphological characteristics of the deposited line" when cultivated under the same cultivation conditions. Specifically, the progeny line may have traits in common with the corresponding deposited line (derived deposited line). As a specific example, the progeny line has, for example, one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, eleven or more, twelve or more, or thirteen or more traits that match those of the corresponding deposited line. The progeny line may be a plant having the main traits of the corresponding deposited line. The above explanation can be applied to the main traits of the corresponding deposited line. The progeny line may be a plant having the same traits as the corresponding deposited line except for, for example, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait, i.e., 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait may be a trait different from the corresponding deposited line. The "trait different from the deposited line" may be a major trait of the corresponding deposited line or a trait other than the major trait of the corresponding deposited line, but a trait other than the major trait of the corresponding deposited line is preferred. The "trait different from the deposited line" can be implemented, for example, by introducing a trait and / or a gene as described below. In the progeny line, all of the traits of the trait numbers 2 to 18 may be the same as those of the corresponding deposited line.

[0073] The progeny line may include cells that contain at least one set of chromosomes derived from the corresponding deposited line. The progeny line may, for example, have at least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of its alleles derived from the corresponding deposited line. That is, the progeny line may have at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% genetic complement with the corresponding deposited line.

[0074] The "allele" is any one or more genes, all of which are associated with a characteristic or trait in a spinach plant. In a diploid cell or organism, the pair of alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

[0075] The genetic complementarity can be calculated, for example, by deciphering a molecular marker or a base sequence, comparing it with the molecular marker or base sequence of the deposited line, and calculating the coincidence rate. Examples of the molecular marker include SNP (single nucleotide polymorphism) markers, amplified fragment length polymorphism (AFLP) markers, restriction fragment length polymorphism (RFLP) markers, microsatellite markers, sequence-characterized amplified region (SCAR) markers, and cleaved amplified polymorphic sequence (CAPS) markers. Genome analysis methods using the molecular markers are well known and widely published (for example, References 3 and 4 below). The base sequence can be deciphered, for example, by extracting a chromosome from the progeny line and sequencing the chromosome. The proportion of alleles derived from the deposited line and the proportion of genetic complementarity may be estimated, for example, from the number of crosses. In this case, the proportion can be estimated from the number of crosses from the deposited line. As a specific example, when the number of crosses from the deposited line is n, the ratio is, for example, (1 / 2) n It can be estimated as ×100%. Reference 3: Sinchan Adhikari et.al, “Application of molecular markers in plant genome analysis: a review”, The Nucleus, 2017, Volume 60, Issue 3, pp. 283-297 Reference 4: Elcio P. Guimaraes et.al., “MARKER-ASSISTED SELECTION Current status and future perspectives in crops, livestock, forestry and fish”, 2007, Springer, 29-49

[0076] The ratio of alleles and genetic complementarity derived from the deposited line is preferably, for example, an average value of the ratios of a plurality of progeny lines. The plurality is, for example, the number of individuals that can be statistically examined, specifically, 200 or more individuals, and preferably 200 to 1000 individuals.

[0077] The progeny line may have SNPs derived from the deposited line. The progeny line may, for example, have at least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of its SNPs derived from the deposited line. That is, the progeny line may have at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of its SNPs identical to the deposited line. In the present invention, when, for example, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or more of the SNPs of a target spinach plant match those of the deposited line, the target spinach plant can be determined (distinguished, estimated, identified, or differentiated) to be a descendant line of the deposited line.

[0078] The progeny line may have, for example, a mutation or an introduced gene. In this case, the progeny line has, for example, one or more traits modified. The progeny line can be produced, for example, by introducing a mutation or an introduced gene into the deposited line or its progeny line. The mutation may be artificially introduced or naturally introduced. The mutation may be, for example, a chemical-induced mutation (e.g., Reference 5 below) or a radiation-induced mutation. The mutation may also be introduced by, for example, a molecular biological technique or a genome editing technique (e.g., Reference 6 below). The introduced gene can be introduced by, for example, a method using Agrobacterium tumefaciens (e.g., Reference 7 below), or the like. Reference 5: Kenji Murakami1 et.al., “Low-oxalate Spinach Mutant Induced by Chemical Mutagenesis”, J. Japan. Soc. Hort. Sci., 2009, vol. 78, No 2, pages 180-184 Reference 6: Yanfei Mao et.al., “Gene editing in plants: progress and challenges”, National Science Review, 2019, vol. 6, pp. 421-437 Reference 7: Dong Poh Chin et.al., “Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants”, Plant Biotechnology, 2009, vol. 26, pages 243-248

[0079] The transgene refers to a desired gene introduced into the genome of a plant, for example, by genetic engineering techniques or traditional breeding methods. The transgene may be derived from, for example, the same species or a different species. The transgene may be a gene containing the same base sequence as that of the species of origin or a gene containing a different base sequence. In the latter case, the different base sequence can be prepared, for example, by performing codon optimization, addition of a transcriptional regulator such as a promoter, or the like, on the same base sequence. The transgene may include a translation region and a non-translation region.

[0080] <Haploid and doubled haploid plants> The spinach plants of the invention may be haploid and / or doubled haploid plants obtained, obtainable or derived from said deposited line. The haploid and / or doubled haploid plants of said deposited line may be used in a method for producing a parent line of said deposited line. In one embodiment, the invention may provide a plant of a haploid and / or doubled haploid plant, a plant part of a haploid and / or doubled haploid plant, or a seed of a haploid and / or doubled haploid plant.

[0081] The doubled haploid plant can be produced by doubling chromosomes in a haploid plant or cell (see, for example, Reference 8 below). As a specific example, haploid pollen is cultured under specific conditions to form plantlets with 1n chromosomes. The plantlets are then treated with a chemical substance such as colchicine to double the chromosomes. As a result, the cells of the plantlets have 2n chromosomes (doubled haploid). The doubled haploid plant and its progeny can be obtained by growing the plantlets after the treatment. Reference 8: Davut Keles et.al., “First Report of Obtaining Haploid Plants Using Tissue Culture Techniques in Spinach”, HORTSCIENCE, 2016, vol. 51, No 6, pages 742-749

[0082] <Method of manufacturing spinach plants> As described above, the method for producing a spinach plant of the present invention includes a crossing step of crossing a first spinach plant with a second spinach plant, the first spinach plant being the spinach plant of the present invention. The method for producing a spinach plant of the present invention is characterized in that the spinach plant of the present invention is used as at least one of the parents in the crossing step, and other steps and conditions are not particularly limited.

[0083] The method for producing a spinach plant of the present invention includes a self-pollination step of selfing the spinach plant of the present invention. The production method of the present invention is characterized by self-pollinating the spinach plant of the present invention, and other steps and conditions are not particularly limited.

[0084] According to the production method of the present invention, progeny lines of the deposited line can be produced. The explanation of the spinach plant of the present invention can be applied to the production method of the present invention.

[0085] In the present invention, the cross between the first spinach plant (first parent line) and the second spinach plant (second parent line) may be, for example, cross between the same individuals (normal self-pollination), cross between the same clone individuals or between individuals of a line maintained as an inbred line (quasi-self-pollination), or cross between different individuals (cross-pollination). In the case of normal self-pollination, for example, one of the first parent line and the second parent line is a female organ in the same individual, and the other is pollen in the same individual.

[0086] In the present invention, the first parent line is the spinach plant of the present invention, for example, the above-mentioned recipient line. Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB The spinach plant deposited under the reference number P-22412 or its progeny.

[0087] The second parent line is not particularly limited, and any spinach plant can be used. The second parent line may be, for example, a spinach plant of the same taxonomic species as the first parent line, or a spinach plant of a different species. The second parent line may be, for example, the deposited line or a progeny line, or another spinach plant.

[0088] The production method of the present invention may further include, for example, a cultivation step of cultivating a progeny line obtained in the hybridization step after the hybridization step. The cultivation conditions in the cultivation step may be, for example, general cultivation conditions for spinach plants.

[0089] The spinach plant of the present invention can be obtained, for example, by the production method of the present invention.

[0090] <Method of producing seeds of spinach plant> The present invention provides a method for producing spinach seeds. The method for producing spinach seeds of the present invention includes the steps of selfing or crossing a spinach plant of the deposited line with another spinach plant, and optionally producing (collecting or harvesting) the resulting seeds. The seed production method of the present invention may provide a plant, plant part or seed by growing the seed of the spinach plant.

[0091] The seed production method of the present invention may be a method for producing seeds derived from the deposited line. In this case, the seed production method of the present invention may include a step of (a) crossing the plant of the deposited line with another spinach plant to produce seeds. The seed production method of the present invention may further include a step of (b) cultivating a spinach plant from the seed of the step (a) to produce a spinach plant derived from the deposited line, and a step of (c) self-pollinating or crossing the spinach plant of the step (b) with another spinach plant to produce an additional spinach plant derived from the deposited line. Furthermore, the seed production method of the present invention may include (d) optionally repeating the steps (b) and (c) one or more times to produce an additional spinach plant derived from the deposited line. In this case, the additional spinach plant obtained in the step (c) can be used as the spinach plant cultivated from the seed of the step (a) in the step (b). The "once or more" is, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The seed production method of the present invention may further include a step of collecting or harvesting the seeds. The seed production method of the present invention may provide the seeds produced by the above method, and a plant or a part of a plant obtained by growing the seeds.

[0092] The seed production method of the present invention may further comprise (e) identifying a progeny line having downy mildew resistance in the production of a spinach plant in the step (b), in the production of an additional spinach plant in the step (c), or in the production of a further spinach plant derived from the deposited line obtained in the step (d). In this case, the seed production method of the present invention preferably uses a progeny line having downy mildew resistance as a spinach plant to be crossed in the subsequent steps. The "identification" may also be referred to as, for example, discrimination, appraisal, identification, selection, or selection. In the step (e), the identification may be performed by an inoculation test of the aforementioned trait number 18. The downy mildew resistance possessed by the progeny line is preferably the same as that of the deposited line from which it is derived, and the above explanation may be applied.

[0093] <Method of producing hybrid spinach plants> The present invention provides a method for producing a hybrid spinach plant. The method for producing a hybrid plant of the present invention comprises the step of crossing a spinach plant of the present invention with another spinach plant. The method for producing a hybrid plant of the present invention may comprise the step of collecting or harvesting seeds obtained by the cross. The method for producing a hybrid plant of the present invention may also provide seeds and hybrid plants or parts of hybrid plant individuals produced by the above methods.

[0094] <Method of introducing new traits> The present invention provides a method for introducing at least one new characteristic or trait (hereinafter, collectively referred to as "trait") into the deposited line. The trait introduction method of the present invention can also be referred to as, for example, a method for producing a spinach plant into which a new trait has been introduced. The trait introduction method of the present invention includes, for example, (a) a step of crossing a plant of the deposited line with a spinach plant containing at least one new trait to produce a progeny line, and (b) a step of selecting a progeny line containing at least one new trait. The trait introduction method of the present invention includes, for example, a step of (c) a step of crossing the progeny line with the deposited line to produce seeds of a backcross progeny, and (d) a step of selecting a backcross progeny having at least one new trait and downy mildew resistance. In the steps (b) and (d), the selection (selection) of a progeny line having a new trait may be performed, for example, by detecting the trait, or by detecting a gene or molecular marker associated (linked) with the trait. The new trait may be, for example, resistance to pathogens other than downy mildew.

[0095] In the step (b), it is preferable to select a spinach plant that includes at least one new trait and exhibits downy mildew resistance. In this case, the spinach plant selected in the step (b) may exhibit resistance to at least one race selected from the group consisting of downy mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, as in the progeny line described above. It is preferable that the spinach plant selected in the step (b) exhibits the same or similar downy mildew resistance as the deposited line in the step (a). As a specific example, when the deposited line is the first downy mildew-resistant spinach plant, it is preferable that the spinach plant selected in the step (b) has the trait (1) described above. When the deposited line is the second mildew-resistant spinach plant, the spinach plant selected in step (b) preferably has the trait (2) or (2') When the deposited line is the third mildew-resistant spinach plant, the spinach plant selected in step (b) preferably has the trait (3) or (3').

[0096] In the step (d), a progeny line having at least one new trait and mildew resistance and essentially all physiological and morphological characteristics of the deposited line in the step (a) may be selected. In this case, the progeny line in the step (d) may exhibit resistance to at least one race selected from the group consisting of mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, as in the above-mentioned progeny line. It is preferable that the progeny line in the step (d) exhibits the same or similar mildew resistance as the deposited line in the step (a). As a specific example, when the deposited line is the first mildew-resistant spinach plant, it is preferable that the progeny line in the step (d) has the trait of the above (1). When the deposited line is the second downy mildew-resistant spinach plant, the progeny line of the step (d) preferably has the trait (2) or (2'). When the deposited line is the third downy mildew-resistant spinach plant, the progeny line of the step (d) preferably has the trait (3) or (3'). The "essentially all physiological and morphological characteristics" can be used in the explanation of the progeny line by replacing "progeny line" with "progeny line of the step (d)".

[0097] In the method for introducing a trait of the present invention, (e) the steps (c) and (d) may be optionally repeated one or more times to produce a spinach plant containing at least one new trait. In this case, in the method for introducing a trait of the present invention, the backcross progeny selected in the step (d) may be used as the progeny line in the step (c). The spinach plant obtained or obtainable in the step (e) may exhibit downy mildew resistance and may further have essentially all of the physiological and morphological characteristics of the deposited line in the step (a). In this case, the spinach plant in the step (e) may exhibit resistance to at least one race selected from the group consisting of downy mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, as in the progeny line described above. The spinach plant in the step (e) preferably exhibits the same or similar mildew resistance as the deposited line in the step (a). As a specific example, when the deposited line is the first mildew-resistant spinach plant, the spinach plant in the step (e) preferably has the trait (1). When the deposited line is the second mildew-resistant spinach plant, the spinach plant in the step (e) preferably has the trait (2) or (2'). When the deposited line is the third mildew-resistant spinach plant, the spinach plant in the step (e) preferably has the trait (3) or (3'). The "essentially all physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "spinach plant obtained or obtainable in the step (e)". The "once or more" is, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The method for introducing a trait of the present invention may include a step of collecting or harvesting seeds. The method for introducing a trait of the present invention may provide seeds produced by the above method, and plants or parts of plant individuals obtained by growing the seeds.

[0098] <Method of introducing transgene> The present invention provides a method for producing a plant derived from the deposited line, which contains at least one new characteristic or trait. The method for introducing a transgene of the present invention can also be referred to as a method for producing a spinach plant having a new trait introduced therein.

[0099] The method for introducing a transgene of the present invention includes, for example, a step of introducing a mutation or transgene that confers at least one new trait into a plant of the deposited line or a progeny line. The introduction of the mutation or transgene can be carried out, for example, in the same manner as the introduction of the mutation or transgene in the progeny line. The spinach plant obtained or obtainable by the introduction step may exhibit downy mildew resistance and may further have essentially all of the physiological and morphological characteristics of the deposited line. In this case, the spinach plant after the introduction step may exhibit resistance to at least one race selected from the group consisting of downy mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, similar to the progeny line described above. The spinach plant after the introduction step preferably exhibits the same or similar downy mildew resistance as the deposited line. As a specific example, when the deposited line is the first mildew-resistant spinach plant, the spinach plant after the introduction step preferably has the trait (1) described above. When the deposited line is the second mildew-resistant spinach plant, the spinach plant after the introduction step preferably has the trait (2) or (2') described above. When the deposited line is the third mildew-resistant spinach plant, the spinach plant after the introduction step preferably has the trait (3) or (3') described above. The "essentially all physiological and morphological characteristics" can be used in the explanation of the progeny line by replacing "progeny line" with "spinach plant obtained or obtainable by the introduction step". The method for introducing an introduced gene of the present invention may include a step of collecting or harvesting seeds. The method for introducing an introduced gene of the present invention may provide seeds produced by the above method, and parts of plants or plant individuals obtained by growing the seeds. The new trait may be, for example, resistance to pathogens other than downy mildew.

[0100] <Regeneration and regeneration method of spinach plants> The present invention provides spinach plants regenerated from cell cultures, tissue cultures, or protoplasts of the deposited line (hereinafter referred to as "regenerants"). The present invention may also provide cell or tissue cultures of regenerable cells, or protoplasts derived from tomato plants of the deposited line. The cells, tissues, or protoplasts may be derived from tissues including leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds, or stems.

[0101] The present invention provides a method for multiplying or propagating a spinach plant of a deposited line. The propagation of the spinach plant of the deposited line may be vegetative propagation of the spinach plant of the deposited line. In this case, the method for regenerating a spinach plant of the present invention includes, for example, (a) collecting tissue capable of propagation from a plant of the deposited line, (b) culturing the tissue to obtain a propagated shoot, and (c) rooting the propagated shoot to obtain a rooted plantlet. The method for regenerating a spinach plant of the present invention may further include (d) optionally growing a plant from the rooted plantlet. For the method of vegetative propagation, see, for example, Reference 9 below. The regeneration method of the present invention may provide, for example, a plantlet, a plant, or a part of a plant individual regenerated (produced) by the above method. The plant may exhibit downy resistance. In this case, the plant may be resistant to at least one race selected from the group consisting of the downy mildew races Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B, as in the progeny line described above. The plant preferably exhibits the same or similar downy mildew resistance as the deposited line. As a specific example, when the deposited line is the first downy mildew-resistant spinach plant, the plant preferably has the trait (1). When the deposited line is the second downy mildew-resistant spinach plant, the plant preferably has the trait (2) or (2'). When the deposited line is the third downy mildew-resistant spinach plant, the plant preferably has the trait (3) or (3'). The plant may also have essentially all of the physiological and morphological characteristics of the deposited line. The term "essentially all of the physiological and morphological characteristics" can be used in the description of the progeny line by substituting "progeny line" with "regenerated plant." Reference 9: Habtamu Gudisa Megersa, “Propagation Methods of Selected Horticultural Crops by Specialized Organs: Review”, Journal of Horticulture, 2017, Volume 4, Issue 2, 1000198

[0102] <Harvested and processed products of spinach plants> The present invention provides harvested and / or processed products of the deposited line or progeny line, the harvested products being whole plants or parts of individual plants, preferably including leaves (e.g., petioles and blades), leaves and roots, or seeds.

[0103] The processed product includes any product obtained by processing the deposited line or progeny line. The processing is not particularly limited, and examples thereof include cutting, slicing, grounding, pureeing, drying, canning, bottling, washing, packaging, freezing and / or heat treatment. In the deposited line or progeny line, the part of the plant or plant individual used for the processed product is, for example, a leaf. The processed product may be, for example, the deposited line or progeny line that has been washed and packaged. The processed product may be, for example, housed in a container of any size or shape. Specific examples of the container include a bag, a box, a carton, and the like.

[0104] The invention may provide a container comprising one or more spinach plants, said container comprising a whole plant or parts of a plant.

[0105] The present invention may provide a method for producing a spinach plant as a food (a method for producing a food). The method for producing a food of the present invention may, for example, comprise a step of collecting or harvesting the whole plant or a part of a plant individual of the deposited line or a progeny line, preferably the leaves, or the leaves and roots, of the deposited line or a progeny line. The method for producing a food of the present invention may also comprise a step of cultivating the spinach plant of the deposited line or a progeny line until it reaches maturity.

[0106] <Method of determining genotype> The present invention provides a method for determining or detecting the genotype of a deposited line or a progeny line. The genotype determination method of the present invention includes, for example, (a) a step of obtaining a nucleic acid sample from the deposited line or a progeny line, and (b) a step of detecting a genome in the nucleic acid sample. In the step (a), the method for preparing nucleic acid from the deposited line or the progeny line can be performed using a general method for preparing nucleic acid from tissue. In the step (b), for example, polymorphisms and / or alleles in the genome in the nucleic acid sample are detected. The detection of the polymorphism and / or allele can be carried out using, for example, SNP (single nucleotide polymorphism) genotyping, amplified fragment length polymorphism detection (AFLP), restriction fragment length polymorphism discrimination (RFLP) of genomic DNA, sequence-characterized amplified region detection (SCAR) of genomic DNA, cleaved amplified polymorphic sequence detection (CAPS) of genomic DNA, random amplified polymorphic detection (RAPD) of genomic DNA, polymerase chain reaction (PCR), DNA sequence, allele specific oligonucleotide (ASO) probe, or DNA microarray. The detection of the polymorphism and / or allele may be carried out, for example, by sequencing the base sequence of the genome, or may be carried out with reference to the SNP of the deposited lineage as described above. In the step (b), one polymorphism and / or allele in the genomic DNA may be detected, or two or more polymorphisms and / or alleles may be detected. The genotyping method of the present invention may include a step of storing the detection results of the polymorphisms and / or alleles in a computer readable medium. The present invention may provide a computer readable medium produced by such a method.

[0107] The genotype determination method of the present invention may be carried out on any spinach plant (target spinach plant) instead of the deposited line or the progeny line, for example. In this case, the genotype determination method of the present invention may further include, for example, a step of determining whether the target spinach plant is the progeny line based on the result of the step (b). The determination may also be referred to as, for example, discrimination, estimation, appraisal, or identification. The determination may be determined, for example, based on the coincidence rate between the result of the step (b) and the genotype of the deposited line. EXAMPLES

[0108] The present invention will be described in detail below using examples, but the present invention is not limited to the embodiments described in the examples.

[0109] [Example 1] Spinach plants of the deposited lines were bred and confirmed for downy mildew resistance and other characteristics and traits.

[0110] (1) Breeding of the deposited strain Wild spinach species were introduced from the Centre for Genetic Resources at the University of Wageningen. The introduced wild species was crossed and / or selfed multiple times with spinach lines owned by Takii Seeds, and in each generation, selection was repeated using Pfs1-17, UUA1014, UA201621A, UA201550, UA201707A, and UA201720B as indicators to select parent lines TK01-TK07 with fixed downy mildew resistance. The parent lines of TK01-TK07 show the same pattern of downy mildew resistance as TK01-TK07, respectively. The parent lines of TK01-TK07 obtained were crossed with spinach lines (susceptible to downy mildew) owned by Takii Seeds, to obtain TK01-TK07. And, TK01 to TK07 are received. Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, receivedEntrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, and Entrust Number FER MB The spinach plant was deposited at NITE-IPOD under the accession number P-22412.

[0111] (2) Characteristics of the deposited strain The characteristics and traits of the plant individuals of the deposited lines were evaluated according to the evaluation criteria for spinach species issued by MAFF. The results are shown in Table 4 below.

[0112] [Table 4]

[0113] (3) Mildew resistance of the accession For the deposited accessions and differential varieties (Viroflay, Resistoflay, Califlay, Clermont, Campania, Boeing, Lazio, Pigeon, Caladonia, Meerkat, and Hydrus), inoculation tests were conducted using each downy mildew race (Pfs1-17, UA1014, UA201621A, UA201550, UA201707A, and UA201720B) according to the evaluation criteria for spinach varieties published by MAFF to evaluate downy mildew resistance.

[0114] The evaluation results of mildew resistance by inoculation test and field cultivation are shown in Tables 5A and B. As shown in Tables 5A and B, the deposited lines showed mildew resistance, and in particular, TK03, TK04, TK05, TK06, and TK07 showed resistance to all races and showed extremely strong mildew resistance.

[0115] [Table 5A]

[0116] [Table 5B]

[0117] Although the present invention has been described above with reference to the embodiments and examples, the present invention is not limited to the above-mentioned embodiments and examples. Various modifications that can be understood by those skilled in the art can be made to the configuration and details of the present invention within the scope of the present invention.

[0118] <Additional Notes> Some or all of the above-described embodiments and examples may be described as follows, but are not limited to the following: (Appendix 1) Receiving Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER M B P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER M B A spinach plant, including the spinach plant identified (as deposited) under P-22412 or a progeny thereof. (Appendix 2) The progeny line is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FERMB P-22411, or Entrust Number FER MB 2. A spinach plant according to claim 1, comprising 50% or more of the alleles of the (deposited) spinach plant identified by P-22412. (Appendix 3) The progeny line is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB contains 50% or more of the alleles of the spinach plant identified in (deposited) P-22412; The progeny line is a spinach plant according to Appendix 1 or 2, having the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 4) The progeny line is a spinach plant according to any one of Supplementary Notes 1 to 3, having the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 5) The spinach plant according to any one of claims 1 to 4, wherein the spinach plant is a plant body or a part thereof. (Appendix 6) 6. The spinach plant of any one of claims 1 to 5, wherein the spinach plant is a seed. (Appendix 7) The method includes a step of crossing a first spinach plant with a second spinach plant, A method for producing a spinach plant, wherein the first spinach plant is a spinach plant described in any one of Appendix 1 to 6. (Appendix 8) seeds of spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07; A representative sample was Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receivingEntrust Number FER MB P-22411, or Entrust Number FER MB A seed, which is a seed of the spinach plant identified (as deposited) with reference to P-22412. (Appendix 9) a spinach plant of spinach variety TK01, TK02, TK03, TK04, TK05, TK06, or TK07; A representative sample was Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB A spinach plant, which is a seed of the spinach plant identified (as deposited) with reference to P-22412. (Appendix 10) A spinach plant or part thereof, The spinach plant or part thereof has essentially all of the physiological and morphological characteristics of the spinach plant of claim 9. (Appendix 11) A progeny spinach plant of the spinach plant according to Appendix 9, The progeny spinach plant contains at least 50% of the alleles of the spinach plant of appendix 9; The progeny spinach plant has resistance to downy mildew. (Appendix 12) The progeny spinach plant according to claim 11, wherein the progeny spinach plant has the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 13) The progeny spinach plant according to claim 11 or 12, wherein the progeny spinach plant has the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 14) 10. A part of a spinach plant as described in Appendix 9. (Appendix 15) A part of a plant according to claim 14, The plant parts include microspores, pollen, ovaries, ovules, embryo sacs, and egg cells. , parts of a plant, including cuttings, roots, stems, leaves, cells or protoplasts. (Appendix 16) A method for producing spinach seeds, The method comprises: The spinach plant according to claim 9 is self-pollinated or crossed with another spinach plant, harvesting the resulting seeds. (Appendix 17) 17. A spinach seed derived from a spinach plant, produced by the method of claim 16. (Appendix 18) A spinach plant or part thereof produced by growing the spinach seed of claim 17. (Appendix 19) 19. The spinach plant or part thereof according to claim 18, The spinach plant or part thereof has the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 20) 19. The spinach plant or part thereof according to claim 18, The spinach plant or part thereof has the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 21) 19. The spinach plant or part thereof according to claim 18, The spinach plant or part thereof may be a representative sample. Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB A seed of a spinach plant identified in (deposited) with P-22412, said seed containing at least 50% of the alleles of spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07; The spinach plant or part thereof has the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronosporaeffusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 22) 19. The spinach plant or part thereof according to claim 18, Receiving Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER M B P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER M B A seed of a spinach plant identified in (deposited) with P-22412, said seed containing at least 50% of the alleles of spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07; The spinach plant or part thereof has the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 23) A spinach plant or part thereof according to any one of claims 18 to 22, The spinach plant or part thereof may be a representative sample. Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB A spinach plant or part thereof having essentially all of the physiological and morphological characteristics of spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07, which is a seed of the spinach plant identified in (deposited) P-22412. (Appendix 24) A spinach plant or part thereof according to any one of claims 18 to 23, The spinach plant or part thereof, wherein the spinach plant or part thereof has been modified in one or more traits. (Appendix 25) 25. A spinach plant or part thereof according to claim 24, A spinach plant or part thereof, wherein said modification is effected by mutagenesis. (Appendix 26) 10. A method for producing seeds of a spinach plant from the spinach plant of claim 9, the method comprising: (a) Reception Entrust Number FER MBP-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB crossing the seed of the deposited spinach plant, identified as spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07, with another spinach plant to produce seed; (b) growing a spinach plant from the seed of step (a) to produce a spinach plant derived from the spinach variety; (c) selfing or crossing the spinach plant of step (b) with another spinach plant to produce an additional spinach plant derived from the spinach variety; (d) optionally repeating steps (b) and (c) one or more times to produce additional spinach plants from said spinach variety, wherein said spinach plants in step (b) are grown from said additional spinach plants of step (c); The method includes: (Appendix 27) A seed produced by the method according to claim 26, The spinach plant grown from the seed has the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronospora effusa) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 28) A seed produced by the method according to claim 26, The spinach plant grown from the seed has the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 29) A seed produced by the method according to claim 26, The seed contains at least 50% alleles of the spinach plant of claim 9; The spinach plant grown from the seed has the following trait (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronosporaeffusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 30) A seed produced by the method according to claim 26, The seed contains at least 50% alleles of the spinach plant of claim 9; The spinach plant grown from the seed has the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4–17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 31) 31. A spinach plant produced by growing a seed of the spinach plant of any of claims 27 to 30. (Appendix 32) A method for introducing at least one new trait into a spinach plant according to claim 9, comprising: (a) Reception Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receivingEntrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB crossing a seed of the deposited spinach plant, identified as spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07, with a spinach plant having at least one new trait to produce progeny; (b) selecting progeny containing at least one new trait; (c) crossing the progeny with spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07 (the cultivar crossed in step (a) above) to produce backcross progeny; (d) selecting a backcross progeny containing at least one new trait and having resistance to downy mildew; (e) optionally repeating steps (c) and (d) one or more times to produce a spinach plant containing at least one new trait and having downy mildew resistance, the spinach plant in step (c) being a selected backcross progeny of step (d); The method includes: (Appendix 33) The method according to claim 32, wherein the mildew resistance in step (c) is the same mildew resistance as that of the spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07 crossed in step (a). (Appendix 34) The method according to claim 32 or 33, wherein the mildew resistance in step (e) is the same mildew resistance as that of the spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07 crossed in step (a). (Appendix 35) A spinach plant produced by the method of any one of claims 32 to 34. (Appendix 36) 1. A method for producing a spinach plant derived from spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07, comprising at least one new trait, The method comprises: Receiving Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER M B introducing a mutation or transgene conferring at least one trait into spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07, which is a seed of a spinach plant identified in US Pat. No. 4,991,333, or P-22412 (as deposited); The method includes: (Appendix 37) A spinach plant produced by the method of claim 36. (Appendix 38) A method for producing spinach leaves as a food product, The method comprises: Harvesting leaves, or leaves and roots, of the spinach plant or its progeny according to claim 9; The method includes: (Appendix 39) A processed product of the spinach plant or its progeny as described in Appendix 9, The processed products include cut, sliced, ground, pureed, dried, canned, bottled, washed, packaged, frozen and / or heat treated leaves. Processed products. (Appendix 40) A method for determining the genotype of a spinach plant or a progeny thereof according to appendix 9, The method comprises: (a) obtaining a nucleic acid sample from a spinach plant or a progeny thereof as described in Appendix 9; (b) detecting a polymorphism in the nucleic acid sample; The method includes: (Appendix 41) A regenerable cell or protoplast tissue culture derived from the spinach plant of claim 9. (Appendix 42) 42. The cultured tissue of claim 41, The cells or protoplasts are derived from leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds or stems. Cultured tissue. (Appendix 43) A spinach plant regenerated from the cultured tissue of claim 41 or 42. (Appendix 44) 44. A spinach plant according to claim 43, The spinach plant is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB a seed of a spinach plant identified by reference number P-22412 (as deposited), said seed having the same downy mildew resistance as spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07; Spinach plant. (Appendix 45) A method for vegetatively propagating a spinach plant according to claim 9, The method comprises: (a) Reception Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MB recovering reproductive tissue from a spinach plant of spinach cultivar TK01, TK02, TK03, TK04, TK05, TK06, or TK07, which is a seed of the spinach plant identified in (deposited) with P-22412; (b) culturing the tissue and obtaining proliferated shoots; (c) rooting the propagated shoots and obtaining rooted plantlets; (d) optionally growing plants from the rooted plantlets; The method includes: (Appendix 46) 46. ​​A spinach plantlet or plant produced by the method of claim 45, The spinach plantlet or plant is Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER M B P-22409, received Entrust Number FER MB P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER MBA seed of the spinach plant identified in (deposited) under US Pat. No. 4,999,333, having essentially all of the physiological and morphological characteristics of spinach cultivars TK01, TK02, TK03, TK04, TK05, TK06, or TK07, Spinach plantlet or plant. (Appendix 47) Receiving Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER M B P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER M B Seeds of the spinach plant, identified as P-22412 (deposited). (Appendix 48) Receiving Entrust Number FER MB P-22406, receiving Entrust Number FER MB P-22407, receiving Entrust Number FER MB P-22408, received Entrust Number FER MB P-22409, received Entrust Number FER M B P-22410, receiving Entrust Number FER MB P-22411, or Entrust Number FER M B A spinach plant grown from seed identified as P-22412 (deposited). (Appendix 49) A progeny line of the spinach plant according to claim 48, The progeny line is a spinach plant having the following traits (1), (2), or (3): (1) Peronospora efusa ( Peronospora effusa ) races Pfs1–17 as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, UA201707A, and UA201720B. vinegar. (Appendix 50) A progeny line of the spinach plant according to claim 48 or 49, The progeny line is a spinach plant having the following trait (2') or (3'): (2') Peronospora efusa ( Peronospora effusa ) resistant to races Pfs1–3, 5–15, and 17 and UA1014, UA201550, UA201707A, and UA201720B, and not resistant to races Pfs4 and Pfs16 and UA201621A; (3') Peronospora efusa ( Peronospora effusa ) races Pfs1 and 4-17, as well as UA1014, UA201621A, UA201550, and UA201707A, UA201720B. It shows no resistance to races Pfs2 and Pfs3. (Appendix 51) A spinach plant comprising a hybrid first generation line of the spinach plant of any one of claims 48 to 50. (Appendix 52) 52. The spinach plant of any one of claims 48 to 51, wherein the spinach plant is a plant body or a part thereof. (Appendix 53) 53. The spinach plant of any of claims 48 to 52, wherein the spinach plant is a seed. (Appendix 54) A method for producing a spinach plant, comprising the step of self-pollinating a spinach plant according to any one of appendices 48 to 53. (Appendix 55) A method for producing a spinach plant, comprising a cross-breeding step of cross-breeding a spinach plant according to any one of appendices 48 to 53 with another spinach plant. (Appendix 56) 56. A method for producing a spinach plant according to claim 54 or 55, comprising a seed harvesting step of harvesting seeds. [Industrial Applicability]

[0119] As described above, the present invention provides a downy mildew-resistant spinach plant, which is extremely useful in the agricultural field, for example, in breeding.

Claims

1. Seeds of the spinach plant deposited under Accession No. FERM BP-22406, Accession No. FERM BP-22407, Accession No. FERM BP-22408, Accession No. FERM BP-22409, Accession No. FERM BP-22410, Accession No. FERM BP-22411, or Accession No. FERM BP-22412.

2. A spinach plant grown from a seed deposited under accession number FERM BP-22406, accession number FERM BP-22407, accession number FERM BP-22408, accession number FERM BP-22409, accession number FERM BP-22410, accession number FERM BP-22411, or accession number FERM BP-22412.

3. The progeny of the spinach plant of claim 2, The progeny line is a spinach plant having the following traits (1), (2), or (3): (1) Resistant to Peronospora effusa races Pfs1 to 17 and UA1014, UA201621A, UA201550, UA201707A, and UA201720B; (2) Resistant to Peronospora effusa races Pfs 1 to 3, 5 to 15, and 17, as well as UA1014, UA201550, UA201707A, and UA201720B; (3) Resistant to Peronospora effusa races Pfs1 and 4-17 and UA1014, UA201621A, UA201550, UA201707A, and UA201720B vinegar.

4. The progeny line of the spinach plant according to claim 2 or 3, The progeny line is a spinach plant having the following trait (2') or (3'): (2') resistant to races Pfs1 to 3, 5 to 15, and 17, as well as UA1014, UA201550, UA201707A, and UA201720B of Peronospora effusa, and not resistant to races Pfs4, Pfs16, and UA201621A; (3') Resistance to Peronospora effusa races Pfs1 and 4-17 and UA1014, UA201621A, UA201550, UA201707A, and UA201720B It shows no resistance to races Pfs2 and Pfs3.

5. A spinach plant comprising a hybrid first generation line of the spinach plant according to any one of claims 2 to 4.

6. The spinach plant according to claim 2 , wherein the spinach plant is a plant body or a part thereof.

7. The spinach plant according to claim 2 , wherein the spinach plant is a seed.

8. A method for producing a spinach plant, comprising a self-pollinating step of self-pollinating the spinach plant according to any one of claims 2 to 7.

9. A method for producing a spinach plant, comprising a cross-breeding step of cross-breeding the spinach plant according to any one of claims 2 to 8 with another spinach plant.

10. The method for producing a spinach plant according to claim 8 or 9, further comprising a seed harvesting step of harvesting seeds.

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