Polypeptides

Abstract

The present invention relates to polypeptides, nucleotides encoding the polypeptides, and methods for producing the polypeptides. The present invention also relates to detergent compositions comprising the polypeptides, laundry methods and uses of the polypeptides.

Description

[Technical Field]

[0001] Sequence Listing Reference This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.

[0002] The present invention relates to novel polypeptides having deoxyribonuclease (DNase) activity, nucleotides encoding the polypeptides, and methods for producing the polypeptides. The invention also relates to detergent compositions containing DNase, laundry methods, and uses of DNase. [Background technology]

[0003] Microorganisms commonly attach alive to surfaces in many natural, industrial, and medical environments and are encapsulated by extracellular substances such as biopolymers and macromolecules. The viscous, encapsulated layer of microorganisms thus formed is called a biofilm. Biofilms are the predominant growth method for bacteria in natural environments, and bacteria growing in biofilms exhibit unique physiological characteristics. Compared to their planktonically growing counterparts, bacteria in biofilms are more resistant to antibiotics, ultraviolet radiation, detergents, and the host immune response.

[0004] Biofilms can contain one or more microorganisms, such as gram-positive and gram-negative bacteria, algae, protozoa, and / or yeast or filamentous fungi, and viruses and / or bacteriophages. Examples of problematic biofilms include dental plaque, infection of medical implants, but also initial contamination of ship hulls. Biofilms are responsible for many infectious diseases in humans and are a significant problem in industry with regard to biofouling of exposed surfaces, where biofilm colonization can form the main component of a localized ecosystem, which can disrupt and interfere with industrial processes and components.

[0005] When laundry items such as T-shirts or sportswear are used, they are exposed to bacteria from the user's body and other environments in which they are used. Some of these bacteria can adhere to the laundry item and form a biofilm on the item. The presence of bacteria means that the laundry item becomes sticky, and therefore dirt adheres to the sticky parts. This dirt has proven difficult to remove with commercially available detergent compositions. Furthermore, when very soiled laundry items are washed together with less soiled laundry items, the dirt present in the wash liquor tends to adhere to the biofilm. As a result, the laundry items become "dirtier" after washing than before washing. Furthermore, these bacteria are also the source of malodors that develop after the laundry items are used. Bad odors (malodors) are difficult to remove and can persist even after washing. The reason for this malodor is the adhesion of bacteria to the surface of the fabric. Due to their adhesion to the fabric, bacteria may remain even after washing and continue to be a source of malodor.

[0006] International Published Applications: WO 2011 / 098579 (University of Newcastle) and WO 2014 / 087011 (Novozymes A / S) relate to deoxyribonuclease compounds and methods for biofilm disruption and prevention. Summary of the Invention [Means for solving the problem]

[0007] The present invention relates to novel polypeptides having DNase (deoxyribonuclease) activity and polynucleotides encoding these polypeptides. One aspect of the invention relates to a composition comprising at least 0.002 ppm of a polypeptide having DNase activity, the polypeptide comprising the motif HXXP (where H is histidine, P is proline, and X is any amino acid), wherein the composition further comprises one or more polyols, preferably selected from the following: glycerol, (mono-, di-, or tri-)propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol, and adonitol; and / or ii. optionally, one or more enzymes, preferably selected from a protease, amylase, or lipase; iii. optionally, one or more surfactants, preferably selected from anionic and nonionic surfactants; iv. Optionally, one or more polymers Includes:

[0008] Another aspect of the present invention is a method for producing a composition comprising: i. a core comprising a polypeptide having DNase activity, and optionally ii. A coating consisting of one or more layers surrounding a core The present invention relates to a granule comprising:

[0009] In one aspect of the invention, the granules comprise a polypeptide having DNase activity, wherein the polypeptide comprises one or more motifs selected from the following motifs: [T / D / S][G / N]PQL (SEQ ID NO: 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) and C[D / N]T[A / R] (SEQ ID NO: 202), and the granules comprise a core comprising the polypeptide and a coating.

[0010] In one aspect, the present invention relates to a composition comprising a polypeptide having DNase activity, wherein the polypeptide comprises one or more motifs selected from the following motifs: [T / D / S][G / N]PQL (SEQ ID NO: 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201), and C[D / N]T[A / R] (SEQ ID NO: 202).

[0011] In one aspect, the present invention relates to a composition wherein the polypeptide having DNase activity belongs to the GYS clade and comprises one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205).

[0012] In one embodiment, a composition comprises a polypeptide, wherein the polypeptide has DNase activity, wherein the polypeptide comprises one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO:204) or ASXNRSKG (SEQ ID NO:205), and wherein the polypeptide comprises, consists essentially of, or consists of an amino acid sequence selected from the group consisting of: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, and SEQ ID NO:80, or a polypeptide having at least 80% sequence identity thereto.

[0013] In one aspect, the composition comprises a polypeptide having DNase activity, the polypeptide belonging to the NAWK clade and comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207).

[0014] In one aspect, a composition comprises a polypeptide, wherein the polypeptide has DNase activity, the polypeptide comprises one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO:206) or NPQL (SEQ ID NO:207), and the polypeptide comprises, consists essentially of, or consists of an amino acid sequence selected from the group consisting of: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116 and SEQ ID NO:119, or a polypeptide having at least 80% sequence identity thereto.

[0015] In one aspect, the composition comprises a polypeptide having DNase activity, the polypeptide belonging to the KNAW clade and comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209).

[0016] In one embodiment, a composition comprises a polypeptide, wherein the polypeptide has DNase activity, the polypeptide comprises P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and the polypeptide comprises, consists essentially of, or consists of an amino acid sequence selected from the group consisting of SEQ ID NO: 122, SEQ ID NO: 125, SEQ ID NO: 128, SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155 and SEQ ID NO: 158, or a polypeptide having at least 80% sequence identity thereto.

[0017] In one aspect, the composition is a cleaning composition, such as a laundry or dishwashing composition.

[0018] One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises the motif HXXP, where H is histidine, P is proline, and X is any amino acid.

[0019] In one embodiment, the polypeptide having DNase activity comprises one or more motifs selected from the group of motifs consisting of: [T / D / S][G / N]PQL (SEQ ID NO: 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) and C[D / N]T[A / R] (SEQ ID NO: 202).

[0020] In one embodiment of the invention, the polypeptide having DNase activity belongs to the GYS clade and comprises one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205).

[0021] In one embodiment, the polypeptide is selected from the group consisting of the polypeptides set forth in the following: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, and SEQ ID NO:80, or a polypeptide having at least 98% sequence identity thereto.

[0022] In one aspect of the present invention, the polypeptide having DNase activity belongs to the NAWK clade and includes a polypeptide comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207).

[0023] In one embodiment, the polypeptide comprises either the motif: [V / I]PL[S / A]NAWK (SEQ ID NO:206) or NPQL (SEQ ID NO:207) and is selected from the group consisting of the polypeptides set forth in the following: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116 and SEQ ID NO:119, and polypeptides having at least 95% sequence identity thereto.

[0024] In one aspect of the invention, the polypeptide having DNase activity belongs to the KNAW clade and comprises one or both of the motifs selected from the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209).

[0025] In one embodiment, the polypeptide comprises the motif: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209) and is selected from the group consisting of the polypeptides set forth in SEQ ID NO: 122, SEQ ID NO: 125, SEQ ID NO: 128, SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155 and SEQ ID NO: 158, or a polypeptide having at least 98% sequence identity thereto.

[0026] One aspect of the present invention relates to a polynucleotide encoding a polypeptide of the present invention. The present invention further relates to a nucleic acid construct or expression vector comprising the polynucleotide. The present invention further relates to a host cell comprising the polypeptide of the present invention.

[0027] One aspect relates to the use of the polypeptides of the invention to reduce or remove biofilm from items such as fabrics, preferably in cleaning processes such as laundry.

[0028] One aspect relates to a method for producing a polypeptide of the invention, comprising: (a) culturing the recombinant host cell under conditions conducive to production of the polypeptide; and (b) recovering the polypeptide Includes:

[0029] The present invention further provides the following: (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, 4, or 6; (b) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5; (c) variants of the mature polypeptide of SEQ ID NO: 2, 4, or 6 containing substitutions, deletions, and / or insertions at one or more positions; and (d) a fragment of the polypeptide of (a), (b), or (c) having DNase activity. Regarding.

[0030] In another aspect, the present invention relates to a detergent composition comprising a polypeptide having DNase activity and, preferably, a detergent adjunct ingredient. One aspect of the present invention relates to a composition comprising a polypeptide having DNase activity, the polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, 4 or 6, and a detergent adjunct.

[0031] The present invention further relates to a method of cleaning or laundering an item, comprising: a. exposing the item to a wash solution comprising a polypeptide having DNase activity or a detergent composition comprising a polypeptide having DNase activity; b. completing at least one cleaning cycle; and c. Optionally, rinse the item. Including, wherein the item is dough and the polypeptide having DNase activity is a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 8, 9 or 10.

[0032] Additionally, the use of DNase to prevent, reduce or remove biofilm on items is also claimed.

[0033] The present invention further relates to nucleotides encoding the polypeptides and methods of producing the polypeptides.

[0034] array SEQ ID NO: 1 DNA sequence obtained from Bacillus sp-62451 SEQ ID NO:2 is the polypeptide sequence derived from SEQ ID NO:1 SEQ ID NO:3 DNA sequence obtained from Bacillus horikoshii SEQ ID NO:4 is a polypeptide sequence derived from SEQ ID NO:3 SEQ ID NO: 5 DNA sequence obtained from Paenibacillus sp-18057 SEQ ID NO:6 is a polypeptide sequence derived from SEQ ID NO:3 SEQ ID NO: 7 Mature polypeptide Benzonase DNase (WO 2011 / 098579) SEQ ID NO: 8 Mature polypeptide of SEQ ID NO: 2 obtained from Bacillus sp-62451 SEQ ID NO: 9 Mature polypeptide of SEQ ID NO: 4 obtained from Bacillus horikoshii SEQ ID NO: 10 Mature polypeptide of SEQ ID NO: 6 obtained from Paenibacillus sp-18057 SEQ ID NO: 11 mature polypeptide obtained from Bacillus sp-62520 SEQ ID NO: 12 mature polypeptide obtained from Bacillus sp-62520 SEQ ID NO: 13 mature polypeptide obtained from Bacillus horikoshii SEQ ID NO: 14 mature polypeptide obtained from Bacillus horikoshii SEQ ID NO: 15 mature polypeptide obtained from Bacillus sp-16840 SEQ ID NO: 16 mature polypeptide obtained from Bacillus sp-16840 SEQ ID NO: 17 mature polypeptide obtained from Bacillus sp-62668 SEQ ID NO: 18 mature polypeptide obtained from Bacillus sp-13395 SEQ ID NO: 19 mature polypeptide obtained from Bacillus horneckiae SEQ ID NO: 20 mature polypeptide obtained from Bacillus sp-11238 SEQ ID NO: 21 mature polypeptide obtained from Bacillus cibi SEQ ID NO: 22 mature polypeptide obtained from Bacillus sp-18318 SEQ ID NO: 23 mature polypeptide obtained from Bacillus idriensis SEQ ID NO: 24 is the Bacillus clausii secretion signal SEQ ID NO: 25 DNA sequence obtained from Bacillus sp-62520 SEQ ID NO:26 polypeptide sequence derived from SEQ ID NO:25 SEQ ID NO: 27 DNA sequence obtained from Bacillus sp-62520 SEQ ID NO:28 polypeptide sequence derived from SEQ ID NO:27 SEQ ID NO: 29 DNA sequence obtained from Bacillus horikoshii SEQ ID NO:30 polypeptide sequence derived from SEQ ID NO:29 SEQ ID NO: 31 DNA sequence obtained from Bacillus horikoshii SEQ ID NO: 32 polypeptide sequence derived from SEQ ID NO: 31 SEQ ID NO: 33 DNA sequence obtained from Bacillus sp-16840 SEQ ID NO: 34 polypeptide sequence derived from SEQ ID NO: 33 SEQ ID NO: 35 DNA sequence obtained from Bacillus sp-16840 SEQ ID NO: 36 polypeptide sequence derived from SEQ ID NO: 35 SEQ ID NO: 37 DNA sequence obtained from Bacillus sp-62668 SEQ ID NO: 38 polypeptide sequence derived from SEQ ID NO: 37 SEQ ID NO: 39 DNA sequence obtained from Bacillus sp-13395 SEQ ID NO: 40 polypeptide sequence derived from SEQ ID NO: 39 SEQ ID NO: 41 DNA sequence obtained from Bacillus horneckiae SEQ ID NO: 42 polypeptide sequence derived from SEQ ID NO: 41 SEQ ID NO: 43 DNA sequence obtained from Bacillus sp-11238 SEQ ID NO: 44 polypeptide sequence derived from SEQ ID NO: 43 SEQ ID NO: 45 DNA sequence obtained from Bacillus cibi SEQ ID NO: 46 polypeptide sequence derived from SEQ ID NO: 45 SEQ ID NO: 47 DNA sequence obtained from Bacillus sp-18318 SEQ ID NO: 48 polypeptide sequence derived from SEQ ID NO: 47 SEQ ID NO: 49 DNA sequence obtained from Bacillus idriensis SEQ ID NO:50 polypeptide sequence derived from SEQ ID NO:49 SEQ ID NO: 51 DNA sequence obtained from Bacillus algicola SEQ ID NO:52 polypeptide sequence derived from SEQ ID NO:51 SEQ ID NO: 53 is the mature polypeptide obtained from Bacillus algicola SEQ ID NO: 54 DNA sequence from Xanthan alkaline community J SEQ ID NO: 55 polypeptide sequence derived from SEQ ID NO: 54 SEQ ID NO: 56 mature polypeptide obtained from xanthan alkaline community J SEQ ID NO: 57 DNA sequence obtained from Bacillus vietnamensis SEQ ID NO:58 polypeptide sequence derived from SEQ ID NO:57 SEQ ID NO: 59 mature polypeptide obtained from Bacillus vietnamensis SEQ ID NO: 60 DNA sequence obtained from Bacillus hwajinpoensis SEQ ID NO: 61 polypeptide sequence derived from SEQ ID NO: 60 SEQ ID NO: 62 mature polypeptide obtained from Bacillus hwajinpoensis SEQ ID NO: 63 DNA sequence obtained from Paenibacillus mucilaginosus SEQ ID NO:64 polypeptide sequence derived from SEQ ID NO:63 SEQ ID NO: 65 mature polypeptide obtained from Paenibacillus mucilaginosus SEQ ID NO: 66 DNA sequence obtained from Bacillus indicus SEQ ID NO:67 polypeptide sequence derived from SEQ ID NO:66 SEQ ID NO: 68 mature polypeptide obtained from Bacillus indicus SEQ ID NO: 69 DNA sequence obtained from Bacillus marisflavi SEQ ID NO: 70 polypeptide sequence derived from SEQ ID NO: 69 SEQ ID NO: 71 mature polypeptide obtained from Bacillus marisflavi SEQ ID NO: 72 DNA sequence obtained from Bacillus luciferensis SEQ ID NO: 73 polypeptide sequence derived from SEQ ID NO: 72 SEQ ID NO: 74 mature polypeptide obtained from Bacillus luciferensis SEQ ID NO: 75 DNA sequence obtained from Bacillus marisflavi SEQ ID NO: 76 polypeptide sequence derived from SEQ ID NO: 75 SEQ ID NO: 77 mature polypeptide obtained from Bacillus marisflavi SEQ ID NO: 78 DNA sequence obtained from Bacillus sp. SA2-6 SEQ ID NO:79 polypeptide sequence derived from SEQ ID NO:78 SEQ ID NO: 80 polypeptide sequence obtained from Bacillus sp. SA2-6 SEQ ID NO: 81 DNA sequence obtained from Pyrenochaetopsis sp. SEQ ID NO: 82 polypeptide sequence derived from SEQ ID NO: 81 SEQ ID NO: 83 mature polypeptide obtained from Pyrenochaetopsis sp. SEQ ID NO: 84 DNA sequence obtained from Vibrissea flavovirens SEQ ID NO: 85 polypeptide sequence derived from SEQ ID NO: 84 SEQ ID NO: 86 mature polypeptide obtained from Vibrissea flavovirens SEQ ID NO: 87 DNA sequence obtained from Setosphaeria rostrata SEQ ID NO: 88 polypeptide sequence derived from SEQ ID NO: 87 SEQ ID NO: 89 mature polypeptide obtained from Setosphaeria rostrata SEQ ID NO: 90 DNA sequence obtained from Endophragmiella valdina SEQ ID NO:91 polypeptide sequence derived from SEQ ID NO:90 SEQ ID NO: 92 mature polypeptide obtained from Endophragmiella valdina SEQ ID NO: 93 DNA sequence obtained from Corynespora cassiicola SEQ ID NO:94 polypeptide sequence derived from SEQ ID NO:93 SEQ ID NO: 95 mature polypeptide obtained from Corynespora cassiicola SEQ ID NO: 96 DNA sequence obtained from Paraphoma sp. XZ1965 SEQ ID NO:97 polypeptide sequence derived from SEQ ID NO:96 SEQ ID NO: 98 mature polypeptide obtained from Paraphoma sp. XZ1965 SEQ ID NO: 99 DNA sequence obtained from Monilinia fructicola SEQ ID NO: 100 polypeptide sequence derived from SEQ ID NO: 99 SEQ ID NO: 101 Mature polypeptide obtained from Monilinia fructicola SEQ ID NO: 102 DNA sequence obtained from Curvularia lunata SEQ ID NO: 103 polypeptide sequence derived from SEQ ID NO: 102 SEQ ID NO: 104 Mature polypeptide obtained from Curvularia lunata SEQ ID NO: 105 DNA sequence obtained from Penicillium reticulisporum SEQ ID NO: 106 polypeptide sequence derived from SEQ ID NO: 105 SEQ ID NO: 107 mature polypeptide obtained from Penicillium reticulisporum SEQ ID NO: 108 DNA sequence obtained from Penicillium quercetorum SEQ ID NO: 109 polypeptide sequence derived from SEQ ID NO: 108 SEQ ID NO: 110 Mature polypeptide obtained from Penicillium quercetorum SEQ ID NO: 111 DNA sequence obtained from Setophaeosphaeria sp. SEQ ID NO: 112 polypeptide sequence derived from SEQ ID NO: 111 SEQ ID NO: 113 mature polypeptide obtained from Setophaeosphaeria sp. SEQ ID NO: 114 DNA sequence obtained from Alternaria sp. XZ2545 SEQ ID NO: 115 polypeptide sequence derived from SEQ ID NO: 114 SEQ ID NO: 116 mature polypeptide obtained from Alternaria sp. XZ2545 SEQ ID NO: 117 DNA sequence obtained from Alternaria SEQ ID NO: 118 polypeptide sequence derived from SEQ ID NO: 117 SEQ ID NO: 119 mature polypeptide obtained from Alternaria SEQ ID NO: 120 DNA sequence obtained from Trichoderma reesei SEQ ID NO: 121 Polypeptide sequence derived from SEQ ID NO: 121 SEQ ID NO: 122 mature polypeptide obtained from Trichoderma reesei SEQ ID NO: 123 DNA sequence obtained from Chaetomium thermophilum SEQ ID NO: 124 polypeptide sequence derived from SEQ ID NO: 123 SEQ ID NO: 125 mature polypeptide obtained from Chaetomium thermophilum SEQ ID NO: 126 DNA sequence obtained from Scytalidium thermophilum SEQ ID NO: 127 polypeptide sequence derived from SEQ ID NO: 126 SEQ ID NO: 128 mature polypeptide obtained from Scytalidium thermophilum SEQ ID NO: 129 DNA sequence obtained from Metapochonia suchlasporia SEQ ID NO: 130 polypeptide sequence derived from SEQ ID NO: 129 SEQ ID NO: 131 Mature polypeptide obtained from Metapochonia suchlasporia DNA sequence SEQ ID NO: 132 obtained from Daldinia fissa SEQ ID NO: 133 polypeptide sequence derived from SEQ ID NO: 132 SEQ ID NO: 134 mature polypeptide obtained from Daldinia fissa SEQ ID NO: 135 DNA sequence obtained from Acremonium sp. XZ2007 SEQ ID NO: 136 polypeptide sequence derived from SEQ ID NO: 135 SEQ ID NO: 137 mature polypeptide obtained from Acremonium sp. XZ2007 SEQ ID NO: 138 DNA sequence obtained from Acremonium dichromosporum SEQ ID NO: 139 polypeptide sequence derived from SEQ ID NO: 138 SEQ ID NO: 140 mature polypeptide obtained from Acremonium dichromosporum SEQ ID NO: 141 DNA sequence obtained from Sarocladium sp. XZ2014 SEQ ID NO: 142 polypeptide sequence derived from SEQ ID NO: 141 SEQ ID NO: 143 mature polypeptide obtained from Sarocladium sp. XZ2014 SEQ ID NO: 144 DNA sequence obtained from Metarhizium sp. HNA15-2 SEQ ID NO: 145 polypeptide sequence derived from SEQ ID NO: 144 SEQ ID NO: 146 mature polypeptide obtained from Metarhizium sp. HNA15-2 SEQ ID NO: 147 DNA sequence obtained from Acremonium sp. XZ2414 SEQ ID NO: 148 polypeptide sequence derived from SEQ ID NO: 147 SEQ ID NO: 149 mature polypeptide obtained from Acremonium sp. XZ2414 SEQ ID NO: 150 DNA sequence obtained from Isaria tenuipes SEQ ID NO: 151 polypeptide sequence derived from SEQ ID NO: 150 SEQ ID NO: 152 mature polypeptide obtained from Isaria tenuipes SEQ ID NO: 153 DNA sequence obtained from Scytalidium circinatum SEQ ID NO: 154 polypeptide sequence derived from SEQ ID NO: 153 SEQ ID NO: 155 mature polypeptide obtained from Scytalidium circinatum SEQ ID NO: 156 DNA sequence obtained from Metarhizium lepidiotae SEQ ID NO: 157 polypeptide sequence derived from SEQ ID NO: 156 SEQ ID NO: 158 mature polypeptide obtained from Metarhizium lepidiotae SEQ ID NO: 159 DNA sequence obtained from Thermobispora bispora SEQ ID NO: 160 polypeptide sequence derived from SEQ ID NO: 159 SEQ ID NO: 161 Mature polypeptide obtained from Thermobispora bispora SEQ ID NO: 162 DNA sequence obtained from Sporormia fimetaria SEQ ID NO: 163 polypeptide sequence derived from SEQ ID NO: 162 SEQ ID NO: 164 mature polypeptide obtained from Sporormia fimetaria SEQ ID NO: 165 DNA sequence obtained from Pycnidiophora cf. dispera SEQ ID NO: 166 polypeptide sequence derived from SEQ ID NO: 165 SEQ ID NO: 167 mature polypeptide obtained from Pycnidiophora cf. dispera SEQ ID NO: 168 DNA sequence obtained from Xanthan alkaline community D SEQ ID NO: 169 polypeptide sequence derived from SEQ ID NO: 168 SEQ ID NO: 170 Mature polypeptide obtained from Xanthan alkaline community D SEQ ID NO: 171 DNA sequence obtained from Xanthan alkaline community O SEQ ID NO: 172 polypeptide sequence derived from SEQ ID NO: 171 SEQ ID NO: 173 mature polypeptide obtained from xanthan alkaline community O SEQ ID NO: 174 DNA sequence obtained from Clavicipitaceae sp-70249 SEQ ID NO: 175 polypeptide sequence derived from SEQ ID NO: 174 SEQ ID NO: 176 mature polypeptide obtained from Clavicipitaceae sp-70249 SEQ ID NO: 177 DNA sequence obtained from Westerdykella sp. AS85-2 SEQ ID NO: 178 polypeptide sequence derived from SEQ ID NO: 177 SEQ ID NO: 179 mature polypeptide obtained from Westerdykella sp. AS85-2 SEQ ID NO: 180 DNA sequence obtained from Humicolopsis cephalosporioides SEQ ID NO: 181 polypeptide sequence derived from SEQ ID NO: 180 SEQ ID NO: 182 mature polypeptide obtained from Humicolopsis cephalosporioides SEQ ID NO: 183 DNA sequence obtained from Neosartorya massa SEQ ID NO: 184 polypeptide sequence derived from SEQ ID NO: 183 SEQ ID NO: 185 mature polypeptide obtained from Neosartorya massa SEQ ID NO: 186 DNA sequence obtained from Roussoella intermedia SEQ ID NO: 187 polypeptide sequence derived from SEQ ID NO: 186 SEQ ID NO: 188 mature polypeptide obtained from 187 SEQ ID NO: 189 DNA sequence obtained from Pleosporales SEQ ID NO: 190 polypeptide sequence derived from SEQ ID NO: 189 SEQ ID NO: 191 mature polypeptide from Pleosporales SEQ ID NO: 192 DNA sequence obtained from the genus Phaeosphaeria SEQ ID NO: 193 polypeptide sequence derived from SEQ ID NO: 192 SEQ ID NO: 194 mature polypeptide obtained from Phaeosphaeria SEQ ID NO: 195 DNA sequence obtained from Didymosphaeria futilis SEQ ID NO: 196 polypeptide sequence derived from SEQ ID NO: 195 SEQ ID NO: 197 Mature polypeptide obtained from Didymosphaeria futilis Sequence number 198 motif [T / D / S][G / N]PQL SEQ ID NO: 199 Motif [G / T]Y[D / S][R / K / L] Sequence number 200 motif [E / D / H]H[I / V / L / F / M]X[P / A / S] Sequence number 201 motif [F / L / Y / I]A[N / R]D[L / I / P / V] Sequence number 202 motif C[D / N]T[A / R] Sequence number 203 motif [D / Q][I / V]DH Sequence number 204 motif [D / M / L][S / T]GYSR[D / N] SEQ ID NO: 205 motif ASXNRSKG Sequence number 206 motif [V / I]PL[S / A]NAWK Sequence number 207 motif NPQL Sequence number 208 motif P[Q / E]L[W / Y] Sequence number 209 motif [K / H / E]NAW

[0035] definition Allelic variant: The term "allelic variant" refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation and can result in polymorphism within a population. Gene mutations can be silent (no change in the encoded polypeptide) or can encode a polypeptide having an altered amino acid sequence. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.

[0036] Biofilm: A biofilm is any community of microorganisms in which cells adhere to one another on a surface, such as a fabric, dishware, or hard surface. These attached cells are often embedded in a self-produced matrix of extracellular polymeric substances (EPS). Biofilm EPS is generally a polymeric aggregate composed of extracellular DNA, proteins, and polysaccharides. Biofilms can form on viable or nonviable surfaces. Microbial cells growing within a biofilm are physiologically distinct from planktonic cells of the same organism, which, in contrast, are single cells that can float or swim in a liquid medium. Bacteria living within a biofilm typically have properties quite different from planktonic bacteria of the same species, as the dense and protected environment of the film allows these bacteria to cooperate and interact in diverse ways. One advantage of such an environment is increased resistance to detergents and antibiotics, due to the dense extracellular matrix and outer layer of cells protecting the interior of the population. During washing, biofilm-producing bacteria can be found from the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp., Staphylococcus epidermidis, and Stenotrophomonas sp.

[0037] Coding sequence: The term "coding sequence" refers to a polynucleotide that directly specifies the amino acid sequence of a polypeptide. The boundaries of the coding sequence are generally determined by an open reading frame, which begins with a start codon such as ATG, GTG, or TTG and ends with a stop codon such as TAA, TAG, or TGA. The coding sequence may be genomic DNA, synthetic DNA, or a combination thereof.

[0038] Color difference (L value): The Lab color space is an opponent color space with dimension L, which means lightness. * L * = 0 indicates the darkest black, and L * = 100 indicates the brightest white. In the context of the present invention, the L value is also called color difference.

[0039] Control sequence: The term "control sequence" refers to a nucleic acid sequence necessary for expression of a polynucleotide encoding a mature polypeptide of the present invention. Each control sequence may be native (i.e., derived from the same gene) or foreign (i.e., derived from different genes) to the polynucleotide encoding the polypeptide, or native or foreign to each other. Such control sequences include, but are not limited to, a leader, polyadenylation sequence, propeptide sequence, promoter, signal peptide sequence, and transcription terminator. At a minimum, control sequences include a promoter, and transcriptional and translational stop signals. Control sequences may be provided with linkers for the purpose of introducing specific restriction sites facilitating ligation of the control sequences with the coding region of the polynucleotide encoding the polypeptide.

[0040] Deep Cleaning: The term "deep cleaning" refers to the disruption or removal of biofilm or components of the biofilm, such as polysaccharides, proteins, DNA, dirt or other components present within the biofilm.

[0041] Detergent adjunct ingredients: Detergent adjunct ingredients are different from the DNase of the present invention. The exact nature of these additional adjunct ingredients and their inclusion levels can vary depending on the physical form of the composition and the type of application for which it is intended. Suitable adjunct ingredients include, but are not limited to, those described below, such as surfactants, builders, flocculating aids, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, hydrogen peroxide sources, preformed peracids, polymeric dispersants, mud stain removal / anti-redeposition agents, brighteners, soap suds suppressors, dyes, fragrances, structural elastomers, fabric softeners, carriers, hydrotropes, builders and co-builders, fabric hueing agents, defoamers, dispersants, processing aids, and / or pigments.

[0042] Detergent Composition: The term "detergent composition" refers to a composition useful in removing unwanted compounds from items to be cleaned, such as fabrics. Detergent compositions can be used in both domestic and institutional cleaning applications, for example, to clean fabrics. The term encompasses all materials / compounds selected for a particular type of desired cleaning composition and product form (e.g., liquid, gel, powder, granular, paste, or spray composition), including, but not limited to, detergent compositions (e.g., liquid and / or solid laundry detergents and delicate detergents; fabric fresheners; fabric softeners; and fabric and laundry pre-spotters / pre-treats). In addition to containing an enzyme of the present invention, the detergent formulation may contain one or more additional enzymes (e.g., proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalases, and mannanases, or any mixtures thereof), and / or detergent adjunct ingredients such as surfactants, builders, chelants or chelating agents, bleach systems or components, polymers, fabric conditioners, suds boosters, suds suppressors, dyes, fragrances, colorfastness inhibitors, optical brighteners, bactericides, mildewcides, soil suspending agents, corrosion inhibitors, enzyme inhibitors or stabilizers, enzyme activators, transferases, hydrolases, oxidoreductases, bluing and fluorescent dyes, antioxidants, and lysing agents.

[0043] DNase (deoxyribonuclease): The term "DNase" refers to a polypeptide having DNase activity that degrades DNA by catalyzing the hydrolytic cleavage of phosphodiester bonds in the DNA backbone. The terms "DNase" and "polypeptide having DNase activity" are used interchangeably throughout this application. For the purposes of the present invention, DNase activity is determined according to the procedure described in Assay I. In one aspect, a polypeptide of the present invention has at least 20%, e.g., at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the DNase activity of the mature polypeptide of SEQ ID NO: 2, 4, or 6, preferably SEQ ID NO: 2. In one embodiment, the polypeptide of the invention has improved DNase activity, for example, whereby the DNase activity of the polypeptide is at least 105%, for example at least 110%, at least 120%, at least 130%, at least 140%, at least 160%, at least 170%, at least 180%, or at least 200% relative to the DNase activity of the mature polypeptide of SEQ ID NO: 2, 4 or 6, preferably SEQ ID NO: 2.

[0044] In preferred embodiments, the DNase activity of the polypeptide is at least 20%, e.g., at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 100%, at least 105%, at least 110%, at least 120%, at least 130%, at least 140%, at least 160%, at least 170%, at least 180%, or at least 200%, relative to the DNase activity of the mature polypeptide of SEQ ID NO:2, as determined according to the procedure described in Assay I.

[0045] Enzyme Detergency Benefits: As used herein, the term "enzyme detergency benefit" is defined as the beneficial effect that an enzyme can add to a detergent compared to the same detergent without the enzyme. Important detergency benefits that enzymes can provide are stain removal with no or little visible soil after washing and / or cleaning (an effect also known as anti-redeposition), prevention or reduction of redeposition of soil liberated in the washing process (an effect also known as whitening), and complete or partial restoration of whiteness to fabrics that were originally white but have acquired a grayish or yellowish appearance after repeated use and washing. Textile care benefits that are not directly related to catalytic stain removal or anti-redeposition of soil are also important to enzyme detergency benefits. Examples of such fabric treatment benefits are preventing or reducing dye transfer from one fabric to another or to other parts of the same fabric (an effect also known as dye transfer prevention or back-soiling prevention); removing protruding or broken fibers from the fabric surface to reduce pilling or removing existing pilling or fuzz (an effect also known as anti-pilling); improving fabric softness; clarifying the color of the fabric; and removing particulate soils trapped in the fibers of the fabric or garment. Enzyme bleaching is a further enzyme detergency benefit, where catalytic activity is generally used to catalyze the formation of bleaching components such as hydrogen peroxide or other peroxides.

[0046] Expression: The term "expression" includes any step involved in producing a polypeptide, including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.

[0047] Expression Vector: The term "expression vector" includes a linear or circular DNA molecule that contains a polynucleotide that encodes a polypeptide and is operably linked to a control sequence that provides for its expression.

[0048] Fragment: The term "fragment" refers to a polypeptide having one or more (several) amino acids deleted from the amino and / or carboxyl terminus of a mature polypeptide or domain; wherein the fragment has DNase activity. In one embodiment, the fragment contains at least 206 amino acid residues (e.g., amino acids 1-206 of SEQ ID NO:2), at least 205 amino acid residues (e.g., amino acids 2-206 of SEQ ID NO:2), or at least 204 amino acid residues (e.g., amino acids 3-206 of SEQ ID NO:2). In one embodiment, the fragment contains at least 206 amino acid residues (e.g., amino acids 1-206 of SEQ ID NO:4), at least 205 amino acid residues (e.g., amino acids 4-206 of SEQ ID NO:4), or at least 204 amino acid residues (e.g., amino acids 3-206 of SEQ ID NO:4). In one embodiment, the fragment comprises at least 206 amino acid residues (e.g., amino acids 1-206 of SEQ ID NO: 6), at least 205 amino acid residues (e.g., amino acids 2-206 of SEQ ID NO: 6), or at least 204 amino acid residues (e.g., amino acids 3-206 of SEQ ID NO: 6).

[0049] Host cell: The term "host cell" refers to any cell type that is susceptible to transformation, transfection, transduction, etc., with a nucleic acid construct or expression vector comprising a polynucleotide of the invention. The term "host cell" encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication.

[0050] Improved cleaning performance: The term "improved cleaning performance" is defined herein as an enzyme that exhibits an increase in the cleaning performance of a detergent composition, for example, by increased soil removal or reduced redeposition, relative to the cleaning performance of the same detergent composition without the enzyme. The term "improved cleaning performance" encompasses cleaning performance in the laundry.

[0051] Isolated: The term "isolated" refers to a substance in a form or setting that does not occur in nature. Non-limiting examples of isolated substances include: (1) any non-naturally occurring substance; (2) any substance, including, but not limited to, any enzyme, mutant, nucleic acid, protein, peptide, or cofactor that is at least partially removed from one, more, or all of the natural components with which it is associated in nature; (3) any substance that has been artificially modified relative to a substance found in nature; or (4) any substance that has been modified by increasing the amount of the substance relative to other components with which it is associated in nature (e.g., recombinant production in a host cell; multiple copies of the gene encoding the substance; and the use of a stronger promoter than that naturally associated with the gene encoding the substance). Isolated substances may be present in a fermentation broth sample; for example, host cells may be genetically modified to express a polypeptide of the invention. Fermentation broth from host cells may contain the isolated polypeptide.

[0052] Laundry: The term "laundering," in both domestic and commercial laundering, refers to the process of treating fabrics with a solution containing the cleaning or detergent composition of the present invention. The laundering process can be carried out, for example, using a domestic or commercial washing machine, or by hand.

[0053] The term "malodor" refers to an undesirable odor on a clean item. A washed item should smell fresh and clean, with no malodor adhering to the item. One example of a malodor is a compound with an unpleasant odor that may be produced by a microorganism. Another example is an unpleasant odor that may be sweat or body odor adhering to an item that has been in contact with a human or animal. Another example of a malodor may be the odor from spices adhering to the item, such as curry or other foreign spices with a strong odor. One way to measure the extent to which an item has acquired a malodor is by using Assay II disclosed herein.

[0054] Mature Polypeptide: The term "mature polypeptide" refers to a polypeptide in its final form following translation and any post-translational modifications, such as N-terminal processing, C-terminal truncation, glycosylation, phosphorylation, etc. In one embodiment, the mature polypeptide is amino acids 29-210 of SEQ ID NO:2, amino acids 29-210 of SEQ ID NO:4, or amino acids 23-202 of SEQ ID NO:6, and amino acids 1-28 of SEQ ID NO:2, amino acids 1-28 of SEQ ID NO:4, and amino acids 1-22 of SEQ ID NO:6 are signal peptides.

[0055] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:26.

[0056] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:28.

[0057] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:30.

[0058] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:32.

[0059] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:34.

[0060] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:36.

[0061] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:38.

[0062] In one embodiment, the mature polypeptide is amino acids 1 to 183 of SEQ ID NO:40.

[0063] In one embodiment, the mature polypeptide is amino acids 1 to 185 of SEQ ID NO:42.

[0064] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:44.

[0065] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:46.

[0066] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:48.

[0067] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:50.

[0068] In one embodiment, the mature polypeptide is amino acids 1 to 182 of SEQ ID NO:52.

[0069] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:55.

[0070] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:58.

[0071] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:61.

[0072] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:64.

[0073] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:67.

[0074] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:70.

[0075] In one embodiment, the mature polypeptide is amino acids 1-184 of SEQ ID NO:73.

[0076] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:76.

[0077] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:79.

[0078] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:82.

[0079] In one embodiment, the mature polypeptide is amino acids 1-190 of SEQ ID NO:85.

[0080] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:88.

[0081] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:91.

[0082] In one embodiment, the mature polypeptide is amino acids 1-190 of SEQ ID NO:94.

[0083] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:97.

[0084] In one embodiment, the mature polypeptide is amino acids 1-186 of SEQ ID NO:100.

[0085] In one embodiment, the mature polypeptide is amino acids 1-190 of SEQ ID NO:103.

[0086] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:106.

[0087] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:109.

[0088] In one embodiment, the mature polypeptide is amino acids 1 to 192 of SEQ ID NO:112.

[0089] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:115.

[0090] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:118.

[0091] In one embodiment, the mature polypeptide is amino acids 1-186 of SEQ ID NO:121.

[0092] In one embodiment, the mature polypeptide is amino acids 1-188 of SEQ ID NO:124.

[0093] In one embodiment, the mature polypeptide is amino acids 1-190 of SEQ ID NO:127.

[0094] In one embodiment, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO:130.

[0095] In one embodiment, the mature polypeptide is amino acids 1-198 of SEQ ID NO:133.

[0096] In one embodiment, the mature polypeptide is amino acids 1-188 of SEQ ID NO:136.

[0097] In one embodiment, the mature polypeptide is amino acids 1-182 of SEQ ID NO:139.

[0098] In one embodiment, the mature polypeptide is amino acids 1-188 of SEQ ID NO:142.

[0099] In one embodiment, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO:145.

[0100] In one embodiment, the mature polypeptide is amino acids 1-188 of SEQ ID NO:148.

[0101] In one embodiment, the mature polypeptide is amino acids 1-186 of SEQ ID NO:151.

[0102] In one embodiment, the mature polypeptide is amino acids 1 to 184 of SEQ ID NO:154.

[0103] In one embodiment, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO:157.

[0104] In one embodiment, the mature polypeptide is amino acids 1-226 of SEQ ID NO:160.

[0105] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:163.

[0106] In one embodiment, the mature polypeptide is amino acids 1 to 193 of SEQ ID NO:166.

[0107] In one embodiment, the mature polypeptide is amino acids 1 to 199 of SEQ ID NO:169.

[0108] In one embodiment, the mature polypeptide is amino acids 1-194 of SEQ ID NO:172.

[0109] In one embodiment, the mature polypeptide is amino acids 1 to 186 of SEQ ID NO:175.

[0110] In one embodiment, the mature polypeptide is amino acids 1-187 of SEQ ID NO:178.

[0111] In one embodiment, the mature polypeptide is amino acids 1-194 of SEQ ID NO:181.

[0112] In one embodiment, the mature polypeptide is amino acids 1-190 of SEQ ID NO:184.

[0113] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:187.

[0114] In one embodiment, the mature polypeptide is amino acids 1-191 of SEQ ID NO:190.

[0115] In one embodiment, the mature polypeptide is amino acids 1-192 of SEQ ID NO:193.

[0116] In one embodiment, the mature polypeptide is amino acids 1-189 of SEQ ID NO:196.

[0117] It is known in the art that host cells expressed by the same polynucleotide can produce a mixture of two different mature polypeptides (i.e., containing different C-terminal and / or N-terminal amino acids). Furthermore, it is also known in the art that different host cells process polypeptides differently, such that one host cell expressing a polynucleotide can produce a different mature polypeptide (e.g., with different C-terminal and / or N-terminal amino acids) compared to another host cell expressing the same polynucleotide. The mature polypeptide of SEQ ID NO:2 is SEQ ID NO:8, the mature polypeptide of SEQ ID NO:4 is SEQ ID NO:9, and the mature polypeptide of SEQ ID NO:6 is SEQ ID NO:10.

[0118] Mature polypeptide coding sequence: The term "mature polypeptide coding sequence" refers to a polynucleotide that encodes a mature polypeptide having DNase activity. In one embodiment, the mature polypeptide coding sequence is nucleotides 85-630 of SEQ ID NO:1, nucleotides 85-630 of SEQ ID NO:3, and nucleotides 67-606 of SEQ ID NO:5.

[0119] Nucleic Acid Construct: The term "nucleic acid construct" refers to a nucleic acid molecule, either single-stranded or double-stranded, which is isolated from a naturally occurring gene or which has been modified to contain segments of nucleic acid in a manner not otherwise found in nature, or which is synthetic, and which includes one or more regulatory sequences.

[0120] Operably linked: The term "operably linked" refers to a construct in which control sequences are positioned in appropriate relation to a coding sequence of a polynucleotide so that the control sequences direct the expression of the coding sequence.

[0121] Pharmaceutical auxiliary ingredient means any pharmaceutical excipient suitable for formulating a pharmaceutical compound.

[0122] Such excipients, carriers, vehicles, etc. are well known to those skilled in the art and are described in texts such as Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., 1985.

[0123] Suitable pharmaceutically acceptable excipients for tablet formulation include, for example, inert diluents such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents such as corn starch or alginic acid; binders such as starch, gelatin or acacia, and lubricants such as magnesium stearate, stearic acid or talc.Tablets can be plain or can be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract, thereby providing a sustained effect over a longer period of time.For example, time-delay materials such as glyceryl monostearate or glyceryl distearate can be used.

[0124] For hard gelatin capsules, the active ingredient can be mixed with an inert solid diluent such as calcium carbonate, calcium phosphate, or kaolin.For soft gelatin capsules, the active ingredient can be mixed with water or an oil medium such as peanut oil, liquid paraffin, or olive oil.

[0125] Suitable excipients for the manufacture of aqueous suspensions include suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth, and gum acacia; dispersing or wetting agents may be naturally occurring phospholipids, such as lecithin, or condensation products of alkylene oxides with fatty acids, such as polyoxyethylene stearate, or condensation products of ethylene oxide with long-chain aliphatic alcohols, such as heptadecaethyleneoxyethanol, or condensation products of ethylene oxide with fatty acids and partial esters derived from hexitols, such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with fatty acids and partial esters derived from hexitol anhydrides, such as polyethylene sorbitan monooleate.

[0126] The aqueous suspensions may also contain one or more preservatives, for example, a benzoate, such as ethyl or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.

[0127] Oily suspensions can be prepared by suspending active ingredients in vegetable oils, such as peanut oil, olive oil, sesame oil or coconut oil, or mineral oils such as liquid paraffin.Oily suspensions can contain thickening agents, such as beeswax, hard paraffin or cetyl alcohol.Sweeteners and flavorings can also be added.These compositions can be preserved by adding antioxidants such as ascorbic acid.

[0128] Remission Values: Wash performance is expressed as the remission value of the soiled swatches. After washing and rinsing, the swatches were spread out and allowed to air dry overnight at room temperature. All washed swatches are evaluated the day after washing. Light reflectance evaluation of the swatches was carried out using a Macbeth Color Eye 7000 reflected light spectrometer with a very small aperture. Measurements were performed in incident light without UV and remission at 460 nm was extracted.

[0129] Sequence identity: The relatedness between two amino acid sequences or two nucleotide sequences is expressed by the parameter "sequence identity." For the purposes of the present invention, the degree of sequence identity between two amino acid sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48:443-453), preferably implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16:276-277), version 5.0.0 or later. The parameters used are a gap opening penalty of 10, a gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The output of the Needle-labeled "longest identity" (obtained using the -nobrief option) is used as the percentage identity, calculated as follows: (equivalent residues × 100) / (length of alignment − total number of gaps in the alignment)

[0130] Stringent conditions: The term "very low stringency conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 25% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 45°C.

[0131] The term "low stringency conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 25% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 50°C.

[0132] The term "moderately stringent conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 35% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 55°C.

[0133] The term "moderate-high stringency conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 35% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 60°C.

[0134] The term "highly stringent conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 50% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 65°C.

[0135] The term "highly stringent conditions" refers to 12-24 hours of standard Southern blotting for probes at least 100 nucleotides in length, followed by prehybridization and hybridization in 5x SSPE, 0.3% SDS, 200 micrograms / ml fragment-treated and modified salmon sperm DNA, and 50% formamide at 42°C. The carrier material is finally washed three times for 15 minutes each in 2x SSC, 0.2% SDS at 70°C.

[0136] Subsequence: The term "subsequence" refers to a polynucleotide having one or more (e.g., several) nucleotides deleted from the 5' and / or 3' end of a mature polypeptide coding sequence; wherein the subsequence encodes a fragment with DNase activity. In one embodiment, the subsequence contains at least 550 nucleotides (e.g., nucleotides 85-630 of SEQ ID NO: 1, 3, or 5), at least 400 nucleotides (e.g., nucleotides 100-500 of SEQ ID NO: 1, 3, or 5), or at least 300 nucleotides (e.g., nucleotides 200-500 of SEQ ID NO: 1, 3, or 5).

[0137] Fabric: The term "fabric" refers to any textile material, such as yarns, yarn intermediates, fibers, nonwoven materials, natural materials, synthetic materials, and any other textile material, fabrics made from these materials, and products (e.g., garments and other articles) made from textiles. Fabrics or textiles may be in the form of knits, wovens, denim, nonwovens, felts, yarns, and toweling. Fabrics may be natural cellulosic materials, including cotton, flax / linen, jute, ramie, sisal, or coir, or man-made cellulosic materials (e.g., derived from wood pulp), including viscose / rayon, cellulose acetate fibers (tricellular), lyocell, or blends thereof. Fabrics or textiles may also be non-cellulosic materials, such as natural polyamides, such as wool, camel, cashmere, mohair, rabbit, and silk, or synthetic polymers, such as nylon, aramid, polyester, acrylic, polypropylene, and spandex / elastane, or blends thereof, as well as blends of cellulosic and non-cellulosic fibers. Examples of blends are blends of cotton and / or rayon / viscose with one or more companion materials such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers), and / or cellulose-containing fibers (e.g., rayon / viscose, ramie, flax / linen, jute, cellulose acetate fibers, lyocell). The fabric may be, for example, a conventional washable laundry, such as a dirty domestic laundry. When the terms fabric or garment are used, the broader term "fabric" is also intended to be included. In the context of the present invention, the term "fabric" also encompasses fabrics.

[0138] Mutant: The term "mutant" refers to a polypeptide having the same enzymatic activity as a parent enzyme, but containing modifications (i.e., substitutions, insertions, and / or deletions) at one or more (e.g., several) positions, e.g., a mutant of the present invention having DNase activity. A substitution refers to the replacement of an amino acid occupying a position with a different amino acid; a deletion refers to the removal of an amino acid occupying a position; and an insertion refers to the addition of an amino acid adjacent to and immediately following the amino acid occupying a position. In the context of the present invention, the identified DNAse mutant retains the enzymatic activity of the parent, i.e., the ability to catalyze the hydrolytic cleavage of phosphodiester bonds in the DNA backbone (deoxyribonuclease activity). In one embodiment, the deoxyribonuclease activity of the mutant is increased relative to the mature polypeptide of the parent DNAse, e.g., SEQ ID NO: 2, 4, or 6. In one embodiment, the polypeptide has DNase activity and the variant is a parent DNase, for example: SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:55, SEQ ID NO:58, SEQ ID NO:61, SEQ ID NO:64, SEQ ID NO:67, SEQ ID NO:70, SEQ ID NO:73, SEQ ID NO:76, SEQ ID NO:79, SEQ ID NO:82, SEQ ID NO:85, SEQ ID NO:88, SEQ ID NO:91, SEQ ID NO:94, SEQ ID NO:97, SEQ ID NO:100, SEQ ID NO:103 , SEQ ID NO:106, SEQ ID NO:109, SEQ ID NO:112, SEQ ID NO:115, SEQ ID NO:118, SEQ ID NO:121, SEQ ID NO:124, SEQ ID NO:127, SEQ ID NO:130, SEQ ID NO:133, SEQ ID NO:136, SEQ ID NO:139, SEQ ID NO:142, SEQ ID NO:145, SEQ ID NO:148, SEQ ID NO:151, SEQ ID NO:154, SEQ ID NO:157, SEQ ID NO:160, SEQ ID NO:163, SEQ ID NO:166, SEQ ID NO:169, SEQ ID NO:172, SEQ ID NO:175, SEQ ID NO:178, SEQ ID NO:181, SEQ ID NO:184, SEQ ID NO:187, SEQ ID NO:190, SEQ ID NO:193 or SEQ ID NO:196.

[0139] Wash Cycle: The term "wash cycle" is defined herein as a washing operation in which fabrics are immersed in wash liquor, some mechanical action is applied to the fabric to release soil and promote the inflow and outflow of wash liquor into the fabric, and finally excess wash liquor is removed. After one or more wash cycles, the fabrics are typically rinsed and dried.

[0140] Washing Solution: The term "washing solution" is defined herein as a solution or mixture of water and detergent ingredients (optionally containing an enzyme of the present invention).

[0141] Wash Time: The term "wash time" is defined herein as the time taken for the entire washing process; that is, the total time for the wash cycle and the rinse cycle.

[0142] Whiteness: The term "whiteness" is defined herein as a broad term that has different meanings in different fields and for different customers. Loss of whiteness can be due, for example, to graying, yellowing, or removal of optical brighteners / hues. Graying and yellowing can occur due to soil redeposition, body soils, e.g., iron and iron ion-derived colorants or migration. Whiteness can include one or more items from the following list: colorant or dye action; incomplete soil removal (e.g., body soils, sebum, etc.); redeposition (graying, yellowing, or other discoloration of an object) (reattachment of removed soil to another part of the fabric, soiled or unsoiled); chemical changes in the fabric during use; and color clarification or whitening.

[0143] nomenclature For purposes of the present invention, the designation [E / Q] means that the amino acid at this position can be glutamic acid (Glu, E) or glutamine (Gln, Q). Similarly, the designation [V / G / A / I] means that the amino acid at this position can be valine (Val, V), glycine (Gly, G), alanine (Ala, A), or isoleucine (Ile, I), as well as other combinations described herein. Unless otherwise limited, the amino acid X is defined as being any of the 20 naturally occurring amino acids. [Brief explanation of the drawings]

[0144] [Figure 1] An alignment of the polypeptides of the present invention within the GYS clade is shown in FIG. [Figure 2] An alignment of the polypeptides of the present invention within the NAWK clade is shown in FIG. [Figure 3] An alignment of the polypeptides of the present invention within the KNAW clade is shown in FIG. DETAILED DESCRIPTION OF THE INVENTION

[0145] The present invention relates to novel polypeptides with deoxyribonuclease (DNase) activity that can be used to prevent, reduce, or remove biofilms on items such as fabrics and / or textiles. A polypeptide with DNase activity or a deoxyribonuclease (DNase) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, thereby degrading DNA. The two terms polypeptide with DNase activity and DNase are used interchangeably.

[0146] Polypeptides An example of a polypeptide with DNase activity is a polypeptide containing the PFAM domain DUF1524 (http: / / pfam.xfam.org / ), "The Pfam protein families database: towards a more sustainable future," R.D. Finn, et. al. Nucleic Acids Research (2016) Database Issue 44: D279-D285. The DUF1524 domain contains a conserved HXXP sequence motif commonly found in nucleases (M.A. Machnicka, et. al. Phylogenomics and sequence-structure-function relationships in the GmrSD family of Type IV restriction enzymes BMC Bioinformatics, 2015, 16, 336). DUF stands for domain of unknown function. For example, polypeptide families containing DUFs are collected together in the Pfam database. The Pfam database provides sequence alignments and hidden Markov models that define the collected protein domains. Protein domains are conserved portions of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a dense three-dimensional structure and is often independently stable and can fold. Many proteins consist of several structural domains. A single domain can appear as a wide variety of proteins.

[0147] A particular DUF can be identified using the prefix DUF followed by a number, such as, for example, 1524. DUF1524 is a family of proteins that all contain the HXXP motif, where H is the amino acid histidine, P is the amino acid proline, and X is any amino acid.

[0148] In one aspect of the present invention, a polypeptide of the present invention having DNase activity comprises a DUF1524 domain. Thus, according to one embodiment, the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises a DUF1524 domain, and the present invention relates to the use of such a DNase for preventing, reducing, or removing biofilms on items such as fabrics and / or textiles. The present invention further relates to compositions comprising a polypeptide having DNase activity, comprising a DUF1524 domain, e.g., HXXP. Such compositions may be, but are not limited to, liquid or powder laundry compositions, tablets, unit doses, sprays, or soap bars.

[0149] In one embodiment, the DNase of the present invention comprises one or more DUF1524 domains, for example, one or both of the novel motifs: [T / D / S][G / N]PQL (SEQ ID NO: 198); [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), where T is threonine, D is aspartic acid, S is serine, G is glycine, N is asparagine, P is proline, Q is glutamine, L is leucine, Y is tyrosine, R is arginine, and K is lysine. That is, amino acids are written in single-letter code. Brackets indicate alternative amino acids within the brackets, which are separated by a vertical bar or, in some cases, without a bar, e.g., [TDS]. Thus, [T / D / S][G / N]PQL means that either T, D, or S can be in the first position, and either G or N can be in the second position, followed by PQL. The motif may then be any of TGPQL, TNPQL, DGPQL, DNPQL, SGPQL or SNPQL. In the motif: [G / T]Y[D / S][R / K / L], the conserved amino acid is Y, preceded by an optional amino acid of G or T and followed by an optional amino acid of D or S. In this case, the motif may be GYD, TYD, GYS or TYS.

[0150] Another domain shared by the DUF1524 polypeptides is [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), which corresponds to positions 87-91 in B. cibi (SEQ ID NO: 21). H88 is a catalytic residue involved in the catalytic activity of DUF1524 and is part of the HXXP motif. Modification of H88 to another amino acid can result in loss of catalytic activity.

[0151] Polypeptides with DNase activity and containing these motifs exhibit particularly excellent deep-cleaning properties, making the polypeptides of the invention with DNase activity particularly effective in removing or reducing biofilms. One aspect of the invention relates to polypeptides with DNase activity, wherein the polypeptides contain one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [T / D / S][G / N]PQL (SEQ ID NO: 198), and [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199). One aspect of the invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [T / D / S][G / N]PQL (SEQ ID NO: 198) and [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), with the proviso that the polypeptide is not a DNase comprising SEQ ID NO: 2 of WO 2015 / 155351.

[0152] One embodiment of the present invention comprises one or more of the DUF1524 domains and motifs: SEQ ID NO: 198, SEQ ID NO: 199 or SEQ ID NO: 200, and the DNase has deep cleaning properties, i.e., the DNase effectively prevents, reduces or removes biofilm on items such as fabrics, textiles and / or hard surfaces.

[0153] As mentioned above, polypeptides of the present invention having DNase activity may contain the structural domain of DUF1524. Another domain, preferably shared by DNases of the present invention, has been identified. This domain has not been described previously and is designated NUC1. In addition to having DNase activity, polypeptides of this domain are characterized by containing specific motifs, such as one or more of the motifs: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202); as mentioned above, the letters represent amino acids in single-letter code, where F is phenylalanine, L is leucine, A is alanine, N is asparagine, D is aspartic acid, I is isoleucine, V is valine, H is histidine, G is glycine, C is cysteine, T is threonine, and R is arginine. Brackets indicate that the amino acids within the brackets are alternatives.

[0154] One embodiment of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: Y). One embodiment of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202), preferably, the motif: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) is located at positions corresponding to positions 110-114 of SEQ ID NO: 21, and / or the motif: C[D / N]T[A / R] is located at positions corresponding to positions 43-46 of SEQ ID NO: 21.

[0155] One embodiment of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or both of the motifs: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) or C[D / N]T[A / R] (SEQ ID NO: 202), with the proviso that the polypeptide is not a DNase comprising SEQ ID NO: 2 of WO 2015 / 155351, and preferably, the motif: [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) is located at positions corresponding to positions 110-114 of SEQ ID NO: 21, and / or the motif: C[D / N]T[A / R] is located at positions corresponding to positions 43-46 of SEQ ID NO: 21.

[0156] The motifs and domains are defined as cross-kingdoms, meaning that the domains and motifs include both fungal and bacterial DNases.It is well known that DNases belonging to different taxonomic lineages may share common structural elements, which can be identified by, for example, comparing the primary structure, such as amino acid sequence, and classifying DNases according to sequence homology.However, shared structural elements can also be identified by comparing the three-dimensional (3D) structures of various DNases.Both methods are applied in the present invention.

[0157] Structural approaches have identified DNases that have distinct taxonomic lineages but share common structural elements within the identified groups, e.g., clades, that share common functionality, which may favor specific biofilms, etc.

[0158] From the NUC1 domain, the inventors have identified a subdomain, designated the NUC1_A domain. In addition to comprising any of the aforementioned domains, polypeptides possessing DNase activity belonging to the NUC1_A domain share a common motif: [D / Q][I / V]DH (SEQ ID NO: 203), which corresponds to amino acids 85-88 of the reference polypeptide (SEQ ID NO: 21). The D at position 85 of SEQ ID NO: 21 is predicted to be involved in binding of a catalytic metal ion cofactor, with the letters defining the amino acid as previously described, and parentheses indicating alternative amino acids. In one embodiment, the present invention relates to a polypeptide comprising the motif: [D / Q][I / V]DH (SEQ ID NO: 203), wherein the polypeptide has DNase activity. In one embodiment, the present invention relates to a polypeptide comprising the motif: [D / Q][I / V]DH (SEQ ID NO: 203), wherein the polypeptide has DNase activity, with the proviso that the polypeptide is not a DNase comprising SEQ ID NO: 2 of WO 2015 / 155351. In one embodiment, the present invention relates to a polypeptide comprising one or more of the motifs selected from the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R] and [D / Q][I / V]DH, wherein the polypeptide has DNase activity. In one embodiment, the invention relates to a polypeptide comprising one or more of the motifs selected from the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R] and [D / Q][I / V]DH, wherein the polypeptide has DNase activity, with the proviso that the polypeptide is not a DNase comprising SEQ ID NO: 2 of WO 2015 / 155351.

[0159] Polypeptides having DNase activity and containing one or more or all of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R] and [D / Q][I / V]DH effectively prevent, remove or reduce biofilms, and DNases are particularly useful in cleaning processes such as laundry and dishwashing. One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide is selected from the group consisting of: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116, SEQ ID NO:119, SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO: a polynucleotide having an amino acid sequence as set forth in SEQ ID NO:152, SEQ ID NO:155, SEQ ID NO:158, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:170, SEQ ID NO:173, SEQ ID NO:176, SEQ ID NO:179, SEQ ID NO:182, SEQ ID NO:185, SEQ ID NO:188, SEQ ID NO:191 and SEQ ID NO:197, or a polynucleotide having at least 80% sequence identity thereto, such as at least 85%, for example at least 90%, for example at least 9 The polypeptides are selected from the group consisting of polypeptides having 5% or 100% sequence identity, and further comprise one or more or all of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH. The motifs are novel and have not been previously described.Therefore, the DNases of the present invention have a new common inventive concept.

[0160] One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide comprises one or more or all of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R] or [D / Q][I / V]DH, and wherein the polypeptide is a) a polypeptide having at least 84% sequence identity to the polypeptide of SEQ ID NO: 8; b) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 9; c) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 11; d) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 12; e) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 13; f) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 14; g) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 15; h) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 16; i) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 17; j) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 18; k) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 19; l) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 20; m) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 21; n) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 22; o) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 23; p) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 53; q) a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 56; r) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 59; s) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 62; t) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 68; u) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 71; v) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO: 74; w) a polypeptide having at least 98% sequence identity with the polypeptide of SEQ ID NO: 77; x) a polypeptide having at least 81% sequence identity to the polypeptide of SEQ ID NO: 83; y) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 86; z) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 89; aa) a polypeptide having at least 91% sequence identity with the polypeptide of SEQ ID NO: 92; bb) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 95; cc) a polypeptide having at least 91% sequence identity with the polypeptide of SEQ ID NO: 98; dd) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 104; ee) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 107; ff) a polypeptide having at least 91.5% sequence identity with the polypeptide of SEQ ID NO: 110; gg) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 113; hh) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO: 116; ii) a polypeptide having at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 119; jj) a polypeptide having at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 128; kk) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 131; ll) a polypeptide having at least 79% sequence identity to the polypeptide of SEQ ID NO: 134; mm) a polypeptide having at least 72% sequence identity with the polypeptide of SEQ ID NO: 137; nn) a polypeptide having at least 77% sequence identity with the polypeptide of SEQ ID NO: 140; oo) a polypeptide having at least 74% sequence identity with the polypeptide of SEQ ID NO: 143; pp) a polypeptide having at least 97% sequence identity to the polypeptide of SEQ ID NO: 146; qq) a polypeptide having at least 71% sequence identity to the polypeptide of SEQ ID NO: 149; rr) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 152; ss) a polypeptide having at least 72% sequence identity with the polypeptide of SEQ ID NO: 155; tt) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 158; uu) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 164; vv) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 167; ww) a polypeptide having at least 87% sequence identity with the polypeptide of SEQ ID NO: 176; xx) a polypeptide having at least 81% sequence identity to the polypeptide of SEQ ID NO: 179; yy) a polypeptide having at least 89% sequence identity to the polypeptide of SEQ ID NO: 182; zz) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 185; aaa) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 188; bbb) a polypeptide having at least 87% sequence identity with the polypeptide of SEQ ID NO: 191; ccc) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 194; ddd) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 197 is selected from the group consisting of:

[0161] The NUC_1A domain was first identified in the present invention and is described above. The domain can be further divided into various clades. A clade is a group of polypeptides clustered together based on homology that traces back to a common ancestor. A polypeptide clade can be visualized as a phylogenetic tree, where a clade is a group of polypeptides that consist of a common ancestor and all of its direct descendants. Example 11 describes the construction of a phylogenetic tree.

[0162] A GYS clade, or GYS clade, is a group of DNases that are all related to the same ancestor and share common properties. Here, an unknown clade includes polypeptides that group together within a domain of the phylogenetic tree, e.g., NUC1_A, that share common properties and are more closely related than other polypeptides within the same domain.

[0163] Polypeptides of the GYS clade share the conserved motif: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), where these letters are amino acids (single-letter codes), X is any amino acid, and parentheses indicate that the amino acid is alternative. Additionally, polypeptides of the GYS clade may contain any of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH.

[0164] One aspect of the invention relates to polypeptides of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptides have DNase activity. In one aspect, the ASXNRSKG motif corresponds to positions 125-133 of SEQ ID NO: 21. In one aspect, the [D / M / L][S / T]GYSR[D / N] motif corresponds to positions 26-32 of SEQ ID NO: 21.

[0165] The GYS clade includes polypeptides having DNase activity set forth in the following: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77 and SEQ ID NO:80.

[0166] The polypeptides having DNase activity shown in SEQ ID NO: 65 and SEQ ID NO: 80 are publicly available sequences with UniProt accession numbers (H6NAU2 and A0A0M2T1U6).

[0167] One aspect of the invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO:204), ASXNRSKG (SEQ ID NO:205), wherein the polypeptide has DNase activity, and the polypeptide is selected from any of the following: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, and SEQ ID NO:77 or a variant thereof having 1 to 25, such as 1 to 20, for example 1 to 15, such as 1 to 10, for example 1 to 5 amino acid alterations (e.g. substitutions).

[0168] One aspect of the invention relates to polypeptides of the GYS clade that contain one or both of the motifs: [D / M / L][S / T]GYSR[D / N], ASXNRSKG, and further contain one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein the polypeptide has DNase activity and the polypeptide has the following sequence: The polypeptide is selected from any of the polypeptides set forth in SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74 and SEQ ID NO:77 or a variant thereof having 1 to 25, for example 1 to 20, for example 1 to 15, for example 1 to 10, for example 1 to 5 amino acid alterations (e.g. substitutions).

[0169] One embodiment of the present invention relates to polypeptides of the GYS clade that have DNase activity and include one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204), ASXNRSKG (SEQ ID NO: 205), with the proviso that the polypeptide is not the polypeptide set forth in SEQ ID NO: 65 or SEQ ID NO: 80.

[0170] One embodiment of the present invention relates to a polypeptide of the GYS clade that has DNase activity, wherein the polypeptide comprises one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204), ASXNRSKG (SEQ ID NO: 205), and the polypeptide is a polypeptide of the following: a) a polypeptide having at least 84% sequence identity to the polypeptide of SEQ ID NO: 8; b) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 9; c) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 11; d) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 12; e) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 13; f) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 14; g) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 15; h) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 16; i) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 17; j) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 18; k) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 19; l) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 20; m) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 21; n) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 22; o) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 23; p) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 53; q) a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 56; r) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 59; s) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 62; t) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 68; u) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 71; v) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO: 74; w) a polypeptide having at least 98% sequence identity with the polypeptide of SEQ ID NO: 77 is selected from.

[0171] One embodiment of the present invention relates to polypeptides of the GYS clade having DNase activity, wherein the polypeptide comprises one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO:204), ASXNRSKG (SEQ ID NO:205), and wherein the polypeptide with DNase activity is selected from the group consisting of: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74 and SEQ ID NO:77 or a polypeptide having at least 99% sequence identity thereto.

[0172] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 8 or a polypeptide having at least 84%, such as at least 85%, for example at least 90%, such as at least 95% or such as at least 100% sequence identity thereto.

[0173] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and the polypeptide is a polypeptide set forth in SEQ ID NO: 9, or a polypeptide having at least 94%, such as at least 95% or such as at least 100% sequence identity thereto.

[0174] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 11, or a polypeptide having at least 92%, such as at least 95%, for example at least 96%, such as at least 98% or such as at least 100% sequence identity thereto.

[0175] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 12, or a polypeptide having at least 92%, such as at least 95%, for example at least 97%, such as at least 98% or such as at least 100% sequence identity thereto.

[0176] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 13, or a polypeptide having at least 97%, such as at least 98%, for example at least 99% or such as at least 100% sequence identity thereto.

[0177] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 14, or a polypeptide having at least 96%, such as at least 97%, for example at least 98%, such as at least 99% or such as at least 100% sequence identity thereto.

[0178] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 15, or a polypeptide having at least 90%, such as at least 93%, for example at least 95%, such as at least 97% or such as at least 100% sequence identity thereto.

[0179] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 16, or a polypeptide having at least 88%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 98% or such as at least 100% sequence identity thereto.

[0180] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 17, or a polypeptide having at least 93%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98% or such as at least 100% sequence identity thereto.

[0181] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 18, or a polypeptide having at least 90%, such as at least 91%, for example at least 93%, such as at least 95%, for example at least 98% or such as at least 100% sequence identity thereto.

[0182] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 21), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 19, or a polypeptide having at least 89%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 98% or such as at least 100% sequence identity thereto.

[0183] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 20, or a polypeptide having at least 90%, such as at least 91%, for example at least 93%, such as at least 95%, for example at least 98% or such as at least 100% sequence identity thereto.

[0184] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 21 or a polypeptide having at least 93%, such as at least 94%, for example at least 95%, such as at least 96%, for example at least 97%, such as at least 98% or such as at least 100% sequence identity thereto.

[0185] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 22, or a polypeptide having at least 90%, such as at least 93%, for example at least 94%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98%, such as at least 99% or such as at least 100% sequence identity thereto.

[0186] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 23, or a polypeptide having at least 93%, such as at least 94%, for example at least 95%, such as at least 96%, for example at least 97%, such as at least 98% or such as at least 100% sequence identity thereto.

[0187] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 53, or a polypeptide having at least 85%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98% or such as at least 100% sequence identity thereto.

[0188] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and the polypeptide is a polypeptide set forth in SEQ ID NO: 56, or a polypeptide having at least 98%, such as at least 99% or such as at least 100% sequence identity thereto.

[0189] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 59, or a polypeptide having at least 94%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98% or such as at least 100% sequence identity thereto.

[0190] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 62, or a polypeptide having at least 85%, such as at least 90%, for example at least 95%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98% or such as at least 100% sequence identity thereto.

[0191] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and the polypeptide is a polypeptide set forth in SEQ ID NO: 68, or a polypeptide having at least 97%, such as at least 98% or for example at least 100% sequence identity thereto.

[0192] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and the polypeptide is the polypeptide set forth in SEQ ID NO: 71, or a polypeptide having at least 99% or 100% sequence identity thereto.

[0193] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 74 or a polypeptide having at least 91%, such as at least 93%, for example at least 94%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98% or such as at least 100% sequence identity thereto.

[0194] One embodiment of the present invention relates to a polypeptide of the GYS clade comprising one or more of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), wherein the polypeptide has DNase activity, and the polypeptide is the polypeptide set forth in SEQ ID NO: 77, or a polypeptide having at least 98%, such as at least 99% or 100% sequence identity thereto.

[0195] Another distinguishable clade is the NAWK clade. The NAWK clade or NAWK clade is a group of DNases that are all related to the same ancestor and share common properties. Here, the unknown clade includes polypeptides that form a group within a domain of the phylogenetic tree, such as NUC1_A, and are thought to share common properties and are more closely related than other polypeptides within the same domain.

[0196] Polypeptides of the NAWK clade share the conserved motif: [V / I]PL[S / A]NAWK (SEQ ID NO:206), or NPQL (SEQ ID NO:207), where these letters are amino acids (single-letter code) and amino acids in parentheses are alternatives. Furthermore, polypeptides of the NAWK clade may contain any of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH.

[0197] One aspect of the invention relates to polypeptides of the NAWK clade comprising one or more of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), wherein the polypeptides have DNase activity. In one aspect, the [VI]PL[S / A]NAWK motif corresponds to positions 87-94 of SEQ ID NO: 68. In one aspect, the NPQL motif corresponds to positions 114-117 of SEQ ID NO: 68.

[0198] The NAWK clade includes polypeptides with DNase activity set forth in the following: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116 and SEQ ID NO:119.

[0199] The polypeptide set forth in SEQ ID NO: 119 shares 99.48% sequence identity with the polypeptide of the UniProt sequence having accession number A0A178DM75.

[0200] One aspect of the invention relates to a polypeptide of the NAWK clade comprising one or both of [V / I]PL[S / A]NAWK (SEQ ID NO:206) or NPQL (SEQ ID NO:207), wherein the polypeptide has DNase activity, and the polypeptide is selected from any of the following polypeptides: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116 and SEQ ID NO:119 or a variant thereof having 1 to 25, such as 1 to 20, for example 1 to 15, for example 1 to 10, for example 1 to 5 amino acid alterations (e.g., substitutions).

[0201] One aspect of the invention relates to polypeptides of the NAWK clade that contain one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), and further include one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH. , wherein the polypeptide has DNase activity, and the polypeptide is selected from any of the following: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116 and SEQ ID NO:119, or a variant thereof having 1 to 25, such as 1 to 20, for example 1 to 15, for example 1 to 10, for example 1 to 5 amino acid modifications (e.g. substitutions).

[0202] One embodiment of the present invention relates to polypeptides of the NAWK clade that have DNase activity and include one or more of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), with the proviso that the polypeptide is not the polypeptide set forth in SEQ ID NO: 119.

[0203] One embodiment of the present invention relates to a polypeptide of the NAWK clade having DNase activity, wherein the polypeptide comprises one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207), and the polypeptide is a polypeptide of the following: a) a polypeptide having at least 81% sequence identity to the polypeptide of SEQ ID NO: 83; b) a polypeptide having at least 88.5% sequence identity with the polypeptide of SEQ ID NO: 86; c) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 89; d) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO: 92; e) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 95; f) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO: 98; g) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 101; h) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 104; i) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 107; j) a polypeptide having at least 91.5% sequence identity to the polypeptide of SEQ ID NO: 110; k) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 113, and l) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 116 is selected from.

[0204] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO:206), NPQL (SEQ ID NO:207), wherein the polypeptide has DNase activity, and the polypeptide is selected from the group consisting of: SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, and SEQ ID NO:116, or a polypeptide having at least 96% sequence identity thereto.

[0205] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 83 or a polypeptide having at least 81%, such as at least 83%, for example at least 85%, such as at least 87%, for example at least 90%, such as at least 93%, for example at least 95%, such as at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0206] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 86 or a polypeptide having at least 88.5%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0207] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and the polypeptide is the polypeptide set forth in SEQ ID NO: 89, or a polypeptide having at least 99% or 100% sequence identity thereto.

[0208] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 92 or a polypeptide having at least 91%, such as at least 92%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0209] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 95 or a polypeptide having at least 90%, such as at least 92%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0210] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 98 or a polypeptide having at least 91%, such as at least 92%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0211] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 101 or a polypeptide having at least 89%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0212] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 68), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 104, or a polypeptide having at least 92%, such as at least 93%, for example at least 95%, such as at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0213] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 107 or a polypeptide having at least 90%, such as at least 92%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0214] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 110, or a polypeptide having at least 91.5%, such as at least 92%, for example at least 93%, such as at least 95%, for example at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0215] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 113, or a polypeptide having at least 93%, such as at least 94%, for example at least 95%, such as at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0216] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and the polypeptide is a polypeptide set forth in SEQ ID NO: 116, or a polypeptide having at least 96%, such as at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0217] One embodiment of the present invention relates to a polypeptide of the NAWK clade comprising one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206), NPQL (SEQ ID NO: 207), wherein the polypeptide has DNase activity, and wherein the polypeptide comprises or consists of the polypeptide set forth in SEQ ID NO: 119.

[0218] The third identifiable clade is the KNAW clade. The KNAW clade, or KNAW clade, is a group of DNases that are all related to the same ancestor and share common properties. Here, the unknown clade includes polypeptides that cluster within a domain of the phylogenetic tree, e.g., NUC1_A, and are thought to share common properties and are more closely related than other polypeptides within the same domain.

[0219] Polypeptides of the KNAW clade share the conserved motifs P[Q / E]L[W / Y] (SEQ ID NO: 208) and [K / H / E]NAW (SEQ ID NO: 209), where the letters are amino acids (single-letter codes) and amino acids in parentheses are alternatives. Furthermore, polypeptides of the KNAW clade may contain any of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], or [D / Q][I / V]DH.

[0220] One aspect of the present invention relates to polypeptides of the NAWK clade that contain one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) and / or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptides have DNase activity.

[0221] The KNAW clade includes polypeptides with DNase activity set forth in the following: SEQ ID NO:122, SEQ ID NO:125, SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:155 and SEQ ID NO:158.

[0222] The polypeptides shown in SEQ ID NO: 122 and SEQ ID NO: 125 are published sequences.

[0223] One aspect of the invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is selected from any of the following polypeptides set forth in SEQ ID NO: 128, SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155 and SEQ ID NO: 158, or a variant thereof having 1 to 25, such as 1 to 20, for example 1 to 15, for example 1 to 10, for example 1 to 5 amino acid alterations (e.g. substitutions).

[0224] One embodiment of the invention is directed to a polynucleotide of the KNAW clade that contains one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and further includes one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH. With respect to a peptide, wherein the polypeptide has DNase activity, the polypeptide is selected from any of the polypeptides set forth in the following: SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:155 and SEQ ID NO:158 or a variant thereof having 1 to 25, such as 1 to 20, for example 1 to 15, such as 1 to 10, for example 1 to 5 amino acid alterations (e.g. substitutions).

[0225] One embodiment of the present invention relates to a polypeptide of the KNAW clade that has DNase activity and comprises one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), with the proviso that the polypeptide is not the polypeptide set forth in SEQ ID NO: 122 and SEQ ID NO: 125, and is not the Trichoderma harzianum DNase set forth in SEQ ID NO: 2 of WO 2015 / 155351.

[0226] One embodiment of the present invention relates to a polypeptide of the KNAW clade having DNase activity, wherein the polypeptide comprises one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), and the polypeptide is a polypeptide of the following: a) a polypeptide having at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 128; b) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 131; c) a polypeptide having at least 79% sequence identity to the polypeptide of SEQ ID NO: 134; d) a polypeptide having at least 72% sequence identity to the polypeptide of SEQ ID NO: 137; e) a polypeptide having at least 77% sequence identity to the polypeptide of SEQ ID NO: 140; f) a polypeptide having at least 74% sequence identity to the polypeptide of SEQ ID NO: 143; g) a polypeptide having at least 97% sequence identity to the polypeptide of SEQ ID NO: 146; h) a polypeptide having at least 71% sequence identity to the polypeptide of SEQ ID NO: 149; i) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO: 152; j) a polypeptide having at least 72% sequence identity to the polypeptide of SEQ ID NO: 155; k) a polypeptide having at least 98% sequence identity with the polypeptide of SEQ ID NO: 158 is selected from.

[0227] One embodiment of the present invention relates to polypeptides of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide having DNase activity is selected from the group consisting of: SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155 and SEQ ID NO: 158, or a polypeptide having at least 98% sequence identity thereto.

[0228] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and the polypeptide is the polypeptide set forth in SEQ ID NO: 128, or a polypeptide having at least 99.5% or 100% sequence identity thereto.

[0229] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 131 or a polypeptide having at least 93%, such as at least 94%, for example at least 95%, such as at least 98%, for example at least 99% or 100% sequence identity thereto.

[0230] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 134 or a polypeptide having at least 79%, such as at least 80%, for example at least 85%, such as at least 86%, for example at least 88%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0231] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 137 or a polypeptide having at least 72%, such as at least 75%, for example at least 80%, such as at least 85%, for example at least 86%, such as at least 88%, for example at least 90%, such as at least 93%, for example at least 95%, such as at least 98%, for example at least 99% or 100% sequence identity thereto.

[0232] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 140 or a polypeptide having at least 77%, such as at least 80%, for example at least 85%, such as at least 86%, for example at least 88%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0233] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 143 or a polypeptide having at least 74%, such as at least 80%, for example at least 85%, such as at least 86%, for example at least 88%, such as at least 90%, for example at least 93%, such as at least 95%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0234] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and the polypeptide is a polypeptide set forth in SEQ ID NO: 146, or a polypeptide having at least 97%, such as at least 98%, such as at least 99% or 100% sequence identity thereto.

[0235] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 149 or a polypeptide having at least 71%, such as at least 75%, for example at least 80%, such as at least 85%, for example at least 86%, such as at least 88%, for example at least 90%, such as at least 93%, for example at least 95%, such as at least 98%, for example at least 99% or 100% sequence identity thereto.

[0236] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide set forth in SEQ ID NO: 152 or a polypeptide having at least 96%, such as at least 97%, for example at least 98%, such as at least 99% or 100% sequence identity thereto.

[0237] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and wherein the polypeptide is a polypeptide as set forth in SEQ ID NO: 155 or a polypeptide having at least 72%, such as at least 75%, for example at least 80%, such as at least 85%, for example at least 86%, such as at least 88%, for example at least 90%, such as at least 93%, for example at least 95%, such as at least 98%, for example at least 99% or 100% sequence identity thereto.

[0238] One embodiment of the present invention relates to a polypeptide of the KNAW clade comprising one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209), wherein the polypeptide has DNase activity, and the polypeptide is the polypeptide set forth in SEQ ID NO: 158, or a polypeptide having at least 98%, such as at least 99% or 100% sequence identity thereto.

[0239] One aspect of the present invention relates to a polypeptide having DNase activity, wherein the polypeptide is a) a polypeptide having at least 84% sequence identity to the polypeptide of SEQ ID NO: 8; b) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 9; c) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 11; d) a polypeptide having at least 92% sequence identity with the polypeptide of SEQ ID NO: 12; e) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 13; f) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 14; g) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 15; h) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 16; i) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 17; j) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 18; k) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 19; l) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 20; m) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 21; n) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 22; o) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 23; p) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 53; q) a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 56; r) a polypeptide having at least 94% sequence identity with the polypeptide of SEQ ID NO: 59; s) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 62; t) a polypeptide having at least 97% sequence identity with the polypeptide of SEQ ID NO: 68; u) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 71; v) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO: 74; w) a polypeptide having at least 98% sequence identity with the polypeptide of SEQ ID NO: 77; x) a polypeptide having at least 81% sequence identity to the polypeptide of SEQ ID NO: 83; y) a polypeptide having at least 89% sequence identity with the polypeptide of SEQ ID NO: 86; z) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO: 89; aa) a polypeptide having at least 91% sequence identity with the polypeptide of SEQ ID NO: 92; bb) a polypeptide having at least 90% sequence identity with the polypeptide of SEQ ID NO: 95; cc) a polypeptide having at least 91% sequence identity with the polypeptide of SEQ ID NO: 98; dd) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 104; ee) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 107; ff) a polypeptide having at least 91.5% sequence identity with the polypeptide of SEQ ID NO: 110; gg) a polypeptide having at least 93% sequence identity to the polypeptide of SEQ ID NO: 113; hh) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO: 116; ii) a polypeptide having at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 119; jj) a polypeptide having at least 99.5% sequence identity to the polypeptide of SEQ ID NO: 128; kk) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 131; ll) a polypeptide having at least 79% sequence identity to the polypeptide of SEQ ID NO: 134; mm) a polypeptide having at least 72% sequence identity with the polypeptide of SEQ ID NO: 137; nn) a polypeptide having at least 77% sequence identity with the polypeptide of SEQ ID NO: 140; oo) a polypeptide having at least 74% sequence identity with the polypeptide of SEQ ID NO: 143; pp) a polypeptide having at least 97% sequence identity to the polypeptide of SEQ ID NO: 146; qq) a polypeptide having at least 71% sequence identity to the polypeptide of SEQ ID NO: 149; rr) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 152; ss) a polypeptide having at least 72% sequence identity with the polypeptide of SEQ ID NO: 155; tt) a polypeptide having at least 93% sequence identity with the polypeptide of SEQ ID NO: 158; uu) a polypeptide having at least 85% sequence identity with the polypeptide of SEQ ID NO: 164; vv) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 167; ww) a polypeptide having at least 99.8% sequence identity with the polypeptide of SEQ ID NO: 170; xx) a polypeptide having at least 97% sequence identity to the polypeptide of SEQ ID NO: 173; yy) a polypeptide having at least 87% sequence identity with the polypeptide of SEQ ID NO: 176; zz) a polypeptide having at least 81% sequence identity with the polypeptide of SEQ ID NO: 179; aaa) a polypeptide having at least 89% sequence identity to the polypeptide of SEQ ID NO: 182; bbb) a polypeptide having at least 96% sequence identity with the polypeptide of SEQ ID NO: 185; ccc) a polypeptide having at least 88% sequence identity with the polypeptide of SEQ ID NO: 188; ddd) a polypeptide having at least 87% sequence identity with the polypeptide of SEQ ID NO: 191; eee) a polypeptide having at least 94% sequence identity to the polypeptide of SEQ ID NO: 194; fff) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 197, and optionally ggg) motif: one or more of [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L], [F / L / Y / I]A[N / R]D[L / I / P / V] or C[D / N]T[A / R] and [D / Q][I / V]DH is selected from the group consisting of:

[0240] In one embodiment of the invention, the DNase is obtained from Bacillus sp.-62451, Bacillus horikoshii, or Paenibacillus sp.-18057. The DNase of the invention comprises the mature polypeptide of SEQ ID NO:2, 4, or 6, or a polypeptide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO:2, 4, or 6, wherein the polypeptide has DNase activity.

[0241] The DNases in the GYS clade described above share similar structural and functional characteristics, such as common motifs, as described above. Preferably, the DNases in the GYS clade are obtained from the genus Bacillus. Individual DNases in the GYS clade are described in detail below.

[0242] DNase may also be obtained from Bacillus sp. sp-62451. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 2 or a closely related polypeptide thereto, for example a polypeptide having at least 60%, such as at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or for example at least 95% sequence identity thereto. The DNase of the present invention may be obtained from a Bacillus, for example, Bacillus sp. sp-62451, and may comprise a polypeptide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:8. Polypeptides including SEQ ID NO:21 (mature polypeptide obtained from Bacillus cibi), SEQ ID NO:22 (mature polypeptide obtained from Bacillus sp. sp-18318), and SEQ ID NO:23 (mature polypeptide obtained from Bacillus idriensis) are, for example, homologous polypeptides having at least 80% identity with SEQ ID NO:8.

[0243] Polypeptides including SEQ ID NO:21 (mature polypeptide obtained from Bacillus cibi), SEQ ID NO:22 (mature polypeptide obtained from Bacillus sp. sp-18318), and SEQ ID NO:23 (mature polypeptide obtained from Bacillus idriensis) are also useful in preventing or removing biofilms on items such as fabrics and / or textiles, as shown in Example 2. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO:21 or a closely related polypeptide. Thus, one aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 21, preferably obtained from Bacillus cibi. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 22 or a closely related polypeptide.One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 22, preferably obtained from Bacillus sp-18318. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 23 or a closely related polypeptide. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 23, which is preferably obtained from Bacillus idriensis.

[0244] The DNase may be obtained from the genus Bacillus, preferably Bacillus horikoshii. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 4, or a closely related polypeptide thereof, for example a polypeptide having at least 60%, such as at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or for example at least 95% sequence identity thereto. The DNase of the present invention may be obtained from a Bacillus species, such as Bacillus horikoshii, and may comprise a polypeptide having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:9.SEQ ID NO: 11 (mature polypeptide obtained from Bacillus sp-62520), SEQ ID NO: 12 (mature polypeptide obtained from Bacillus sp-62520), SEQ ID NO: 13 (mature polypeptide obtained from Bacillus horikoshii), SEQ ID NO: 14 (mature polypeptide obtained from Bacillus horikoshii), Homologous polypeptides included in SEQ ID NO: 15 (mature polypeptide obtained from Bacillus sp-16840), SEQ ID NO: 16 (mature polypeptide obtained from Bacillus sp-16840), SEQ ID NO: 17 (mature polypeptide obtained from Bacillus sp-62668), SEQ ID NO: 18 (mature polypeptide obtained from Bacillus sp-13395), SEQ ID NO: 19 (mature polypeptide obtained from Bacillus horneckiae) or SEQ ID NO: 20 (mature polypeptide obtained from Bacillus sp-11238) are homologous polypeptides having at least 80% sequence identity to SEQ ID NO: 9.SEQ ID NO: 11 (mature polypeptide obtained from Bacillus sp-62520), SEQ ID NO: 12 (mature polypeptide obtained from Bacillus sp-62520), SEQ ID NO: 13 (mature polypeptide obtained from Bacillus horikoshii), SEQ ID NO: 14 (mature polypeptide obtained from Bacillus horikoshii), Polypeptides comprising SEQ ID NO: 11 (mature polypeptide obtained from Bacillus sp-16840), SEQ ID NO: 15 (mature polypeptide obtained from Bacillus sp-16840), SEQ ID NO: 16 (mature polypeptide obtained from Bacillus sp-16840), SEQ ID NO: 17 (mature polypeptide obtained from Bacillus sp-62668), SEQ ID NO: 18 (mature polypeptide obtained from Bacillus sp-13395), SEQ ID NO: 19 (mature polypeptide obtained from Bacillus horneckiae), or SEQ ID NO: 20 (mature polypeptide obtained from Bacillus sp-11238) are also useful in preventing or removing biofilms on items such as fabrics and / or textiles, as shown in Example 2. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 11, or a closely related polypeptide thereof. Thus, one aspect of the present invention relates to a DNase having at least 60%, such as at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 11, which is preferably obtained from Bacillus sp.-62520.The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 12, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 12, which is preferably obtained from Bacillus sp.-62520. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 13, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 13, preferably obtained from Bacillus horikoshii. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 14, or a polypeptide closely related thereto.One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 14, preferably obtained from Bacillus horikoshii. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 15 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 15, preferably obtained from Bacillus sp-16840. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 16, or a closely related polypeptide thereof.One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 16, preferably obtained from Bacillus sp-16840. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 17, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 17, preferably obtained from Bacillus sp. 62668. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 18, or a closely related polypeptide thereof.One embodiment of the present invention is a method for producing a nucleic acid sequence comprising SEQ ID NO: 18 and a nucleic acid sequence comprising at least 60%, for example, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least. With respect to DNases having 99% or 100% sequence identity, this is preferably obtained from Bacillus sp-13395. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 19 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 19, which is preferably obtained from Bacillus horneckiae.

[0245] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 20, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 20, which is preferably obtained from Bacillus sp-11238.

[0246] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 53, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 53, preferably obtained from Bacillus algicola.

[0247] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 56, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 56, preferably obtained from a Xanthan alkaline community J.

[0248] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 59 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 59, preferably obtained from Bacillus vietnamensis.

[0249] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 62, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 62, preferably obtained from Bacillus hwajinpoensis.

[0250] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 68, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 68, preferably obtained from Bacillus indicus.

[0251] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 71, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 71, preferably obtained from Bacillus marisflavi.

[0252] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 74, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 74, preferably obtained from Bacillus luciferensis.

[0253] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 77 or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 77, preferably obtained from Bacillus marisflavi.

[0254] The DNases in the NAWK clade described above share similar structural and functional characteristics, such as common motifs, as described above. DNases in the NAWK clade may be obtained from any of the genera and species listed below. Individual DNases in the NAWK group are described in detail below.

[0255] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 83, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 83, which is preferably obtained from Pyrenochaetopsis sp.

[0256] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 86, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 86, preferably obtained from Vibrissea flavovirens.

[0257] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 89, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 89, preferably obtained from Setosphaeria rostrata.

[0258] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 92, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 92, preferably obtained from Endophragmiella valdina.

[0259] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 95, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 95, preferably obtained from Corynespora cassiicola.

[0260] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 98, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 98, preferably obtained from Paraphoma sp. XZ1965.

[0261] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 101, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 101, preferably obtained from Monilinia fructicola.

[0262] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 104, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 104, preferably obtained from Curvularia lunata.

[0263] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 107, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 107, preferably obtained from Penicillium reticulisporum.

[0264] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 110, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 110, preferably obtained from Penicillium quercetorum.

[0265] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 113, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 113, preferably obtained from Setophaeosphaeria sp.

[0266] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 116 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 116, which is preferably obtained from Alternaria sp. XZ2545.

[0267] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 119 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 119, which is preferably obtained from an Alternaria sp.

[0268] The DNases in the KNAW clade described above share similar structural and functional characteristics, such as common motifs, as described above. DNases in the NAWK clade may be obtained from any of the genera and species listed below. Individual DNases in the NAWK group are described in detail below.

[0269] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 128, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 128, preferably obtained from Scytalidium thermophilum.

[0270] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 131, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 131, preferably obtained from Metapochonia suchlasporia.

[0271] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 134, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 134, preferably obtained from Daldinia fissa.

[0272] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 137 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 137, which is preferably obtained from Acremonium sp. XZ2007.

[0273] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 140, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 140, preferably obtained from Acremonium dichromosporum.

[0274] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 143, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 143, which is preferably obtained from Sarocladium sp. XZ2014.

[0275] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 146, or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 146, which is preferably obtained from Metarhizium sp. HNA15-2.

[0276] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 149 or a closely related polypeptide thereof. One aspect of the present invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 149, which is preferably obtained from Acremonium sp. XZ2414.

[0277] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 152, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 152, preferably obtained from Isaria tenuipes.

[0278] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 155, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 155, preferably obtained from Scytaliodium circinatum.

[0279] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 158, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 158, preferably obtained from Metarhizium lepidiotae.

[0280] The following polypeptides with DNase activity are also useful for deep cleaning, e.g., preventing, reducing, or removing biofilms on fabrics, such as cotton and polyester fabrics. The following polypeptides with DNase activity contain NUC1 and NUC1_A domains and NUC1 and NUC1_A motifs and share similarity with polypeptides belonging to any of the clades GYS, NAWK, and KNAW, which also contain NUC1 and NUC1_A domains and motifs.

[0281] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 164, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 164, preferably obtained from Sporormia fimetaria.

[0282] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 167, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 167, preferably obtained from Pycnidiophora cf. dispera.

[0283] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 170, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 170, preferably obtained from a Xanthan alkaline community D.

[0284] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 173, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 173, preferably obtained from a Xanthan alkaline community O.

[0285] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 176, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 176, preferably obtained from Clavicipitaceae sp. 70249.

[0286] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 179 or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 179, preferably obtained from Westerdykella sp. AS85-2.

[0287] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 182, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 182, preferably obtained from Humicolopsis cephalosporioides.

[0288] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 185, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 185, preferably obtained from Neosartorya massa.

[0289] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 188, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 188, preferably obtained from Roussoella intermedia.

[0290] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 191, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 191, preferably obtained from the order Pleosporales.

[0291] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 194, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 194, preferably obtained from a Phaeosphaeria sp.

[0292] The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 197, or a closely related polypeptide thereof. One aspect of the invention relates to a DNase having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 197, preferably obtained from Didymosphaeria futilis.

[0293] The DNase may be obtained from Paenibacillus, preferably Paenibacillus sp-18057. The DNase may be a polypeptide comprising the mature polypeptide of SEQ ID NO: 6, or a closely related polypeptide thereof. The DNase of the present invention may be obtained from Paenibacillus sp-18057 and comprises a polypeptide having at least 60%, e.g., at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:10.

[0294] In one aspect of the present invention, the polypeptide having DNase activity is obtained from a Bacillus species, particularly Bacillus sp-62451. In one aspect of the present invention, the polypeptide having DNase activity is obtained from a Bacillus species, particularly Bacillus horikoshii. In one aspect of the present invention, the polypeptide having DNase activity is obtained from a Bacillus species, particularly Bacillus sp-62520. In one aspect of the present invention, the polypeptide having DNase activity is obtained from a Bacillus species, particularly Bacillus sp-16840. In one aspect of the present invention, the polypeptide having DNase activity is obtained from a Bacillus species, particularly Bacillus sp-62668. In one aspect of the present invention, the polypeptide having DNase activity is obtained from the genus Bacillus, particularly Bacillus sp-13395. In one aspect of the present invention, the polypeptide having DNase activity is obtained from the genus Bacillus, particularly Bacillus sp-11238. In one aspect of the present invention, the polypeptide having DNase activity is obtained from the genus Bacillus, particularly Bacillus cibi. In one aspect of the present invention, the polypeptide having DNase activity is obtained from the genus Bacillus, particularly Bacillus sp-18318. In one aspect of the present invention, the polypeptide having DNase activity is obtained from the genus Bacillus, particularly Bacillus idriensis. In one aspect of the invention, the polypeptide having DNase activity is obtained from Vibrissea flavovirens and comprises or consists of the polypeptide set forth in SEQ ID NO:86.In one aspect of the invention, a polypeptide having DNase activity is obtained from Acremonium dichromosporum and comprises or consists of the polypeptide set forth in SEQ ID NO: 140. In one aspect of the invention, a polypeptide having DNase activity is obtained from Clavicipitaceae sp.-70249 and comprises or consists of the polypeptide set forth in SEQ ID NO: 176. In one aspect of the invention, a polypeptide having DNase activity is obtained from Penicillium reticulisporum and comprises or consists of the polypeptide set forth in SEQ ID NO: 107. In one aspect of the invention, a polypeptide having DNase activity is obtained from Pycnidiophora cf. dispera and comprises or consists of the polypeptide set forth in SEQ ID NO: 167. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Metapochonia suchlasporia and comprises or consists of the polypeptide set forth in SEQ ID NO: 131. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Acremonium sp. XZ2007 and comprises or consists of the polypeptide set forth in SEQ ID NO: 137. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Setosphaeria rostrata and comprises or consists of the polypeptide set forth in SEQ ID NO: 89. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Sarocladium sp. XZ2014 and comprises or consists of the polypeptide set forth in SEQ ID NO: 143. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Metarhizium sp. HNA15-2 and comprises or consists of the polypeptide set forth in SEQ ID NO: 146.In one aspect of the present invention, a polypeptide having DNase activity is obtained from Endophragmiella valdina and comprises or consists of the polypeptide set forth in SEQ ID NO: 92. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Humicolopsis cephalosporioides and comprises or consists of the polypeptide set forth in SEQ ID NO: 182. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Corynespora cassiicola and comprises or consists of the polypeptide set forth in SEQ ID NO: 95. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Paraphoma sp. XZ1965 and comprises or consists of the polypeptide set forth in SEQ ID NO: 98. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Curvularia lunata and comprises or consists of the polypeptide set forth in SEQ ID NO: 104. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Acremonium sp. XZ2414 and comprises or consists of the polypeptide set forth in SEQ ID NO: 149. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Isaria tenuipes and comprises or consists of the polypeptide set forth in SEQ ID NO: 152. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Roussoella intermedia and comprises or consists of the polypeptide set forth in SEQ ID NO: 188. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Scytaliodium circinatum and comprises or consists of the polypeptide set forth in SEQ ID NO: 155.In one aspect of the present invention, a polypeptide having DNase activity is obtained from Setophaeosphaeria sp. and comprises or consists of the polypeptide set forth in SEQ ID NO: 158. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Alternaria sp. XZ2545 and comprises or consists of the polypeptide set forth in SEQ ID NO: 116. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Alternaria sp. and comprises or consists of the polypeptide set forth in SEQ ID NO: 119. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Metarhizium lepidiotae and comprises or consists of the polypeptide set forth in SEQ ID NO: 158. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Pleosporales and comprises or consists of the polypeptide set forth in SEQ ID NO: 191. In one aspect of the invention, a polypeptide having DNase activity is obtained from the genus Phaeosphaeria and comprises or consists of the polypeptide set forth in SEQ ID NO: 194. In one aspect of the invention, a polypeptide having DNase activity is obtained from Didymosphaeria futilis and comprises or consists of the polypeptide set forth in SEQ ID NO: 197. In one aspect of the invention, a polypeptide having DNase activity is obtained from Bacillus vietnamensis and comprises or consists of the polypeptide set forth in SEQ ID NO: 59. In one aspect of the invention, a polypeptide having DNase activity is obtained from Bacillus hwajinpoensis and comprises or consists of the polypeptide set forth in SEQ ID NO: 62.In one aspect of the present invention, a polypeptide having DNase activity is obtained from a Xanthan alkaline community J and comprises or consists of the polypeptide set forth in SEQ ID NO: 56. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Bacillus indicus and comprises or consists of the polypeptide set forth in SEQ ID NO: 68. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Bacillus marisflavi and comprises or consists of the polypeptide set forth in SEQ ID NO: 71. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Bacillus luciferensis and comprises or consists of the polypeptide set forth in SEQ ID NO: 74. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Bacillus marisflavi and comprises or consists of the polypeptide set forth in SEQ ID NO: 77. In one aspect of the invention, a polypeptide having DNase activity is obtained from Sporormia fimetaria and comprises or consists of the polypeptide set forth in SEQ ID NO: 164. In one aspect of the invention, a polypeptide having DNase activity is obtained from Daldinia fissa and comprises or consists of the polypeptide set forth in SEQ ID NO: 134. In one aspect of the invention, a polypeptide having DNase activity is obtained from Pyrenochaetopsis sp. and comprises or consists of the polypeptide set forth in SEQ ID NO: 83. In one aspect of the invention, a polypeptide having DNase activity is obtained from Westerdykella sp. AS85-2 and comprises or consists of the polypeptide set forth in SEQ ID NO: 179.In one aspect of the present invention, a polypeptide having DNase activity is obtained from Monilinia fructicola and comprises or consists of the polypeptide set forth in SEQ ID NO: 101. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Neosartorya massa and comprises or consists of the polypeptide set forth in SEQ ID NO: 185. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Penicillium quercetorum and comprises or consists of the polypeptide set forth in SEQ ID NO: 110. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Xanthan alkaline community D and comprises or consists of the polypeptide set forth in SEQ ID NO: 170. In one aspect of the present invention, a polypeptide having DNase activity is obtained from Bacillus algicola and comprises or consists of the polypeptide set forth in SEQ ID NO: 53. In one aspect of the present invention, the polypeptide having DNase activity is xanthan gum. The polypeptide having DNase activity is obtained from Xanthan alkaline community O and comprises or consists of the polypeptide set forth in SEQ ID NO: 173. In one embodiment of the present invention, the polypeptide having DNase activity is obtained from Scytalidium thermophilum and comprises or consists of the polypeptide set forth in SEQ ID NO: 128.

[0295] In one embodiment of the present invention, the polypeptide having DNase activity is obtained from Paenibacillus, particularly Paenibacillus sp-18057. In one embodiment of the present invention, the polypeptide having DNase activity is obtained from Bacillus and comprises the mature polypeptide of SEQ ID NO: 2, 4, or 6, i.e., the mature polypeptide of SEQ ID NO: 8, 9, or 10. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-62451 and comprises the polypeptide sequence of SEQ ID NO: 8. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus horikoshii and comprises the polypeptide sequence of SEQ ID NO: 9. In a preferred embodiment of the present invention, the DNase is obtained from Paenibacillus sp-18057 and comprises any of the polypeptide sequences of SEQ ID NO: 10. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-62520 and comprises the polypeptide sequence of SEQ ID NO: 11. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-62520 and comprises the polypeptide sequence of SEQ ID NO: 12. In a preferred embodiment of the invention, the DNase is obtained from Bacillus horikoshii and comprises the polypeptide sequence of SEQ ID NO: 13. In a preferred embodiment of the invention, the DNase is obtained from Bacillus horikoshii and comprises the polypeptide sequence of SEQ ID NO: 14. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-16840 and comprises the polypeptide sequence of SEQ ID NO: 15. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-16840 and comprises the polypeptide sequence of SEQ ID NO: 16. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-62668 and comprises the polypeptide sequence of SEQ ID NO: 17. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-13395 and comprises the polypeptide sequence of SEQ ID NO: 18.In a preferred embodiment of the invention, the DNase is obtained from Bacillus horneckiae and comprises the polypeptide sequence of SEQ ID NO: 19. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-11238 and comprises the polypeptide sequence of SEQ ID NO: 20. In a preferred embodiment of the invention, the DNase is obtained from Bacillus cibi and comprises the polypeptide sequence of SEQ ID NO: 21. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-18318 and comprises the polypeptide sequence of SEQ ID NO: 22. In a preferred embodiment of the invention, the DNase is obtained from Bacillus idriensis and comprises the polypeptide sequence of SEQ ID NO: 23.

[0296] In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-62451 and consists of the polypeptide sequence of SEQ ID NO: 8. In another preferred embodiment of the present invention, the DNase is obtained from Bacillus horikoshii and consists of the polypeptide sequence of SEQ ID NO: 9. In another preferred embodiment of the present invention, the DNase is obtained from Paenibacillus sp-18057 and consists of the polypeptide sequence of SEQ ID NO: 10. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-62520 and consists of the polypeptide sequence of SEQ ID NO: 11. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-62520 and consists of the polypeptide sequence of SEQ ID NO: 12. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus horikoshii and consists of the polypeptide sequence of SEQ ID NO: 13. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus horikoshii and consists of the polypeptide sequence of SEQ ID NO: 14. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-16840 and consists of the polypeptide sequence of SEQ ID NO: 15. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-16840 and consists of the polypeptide sequence of SEQ ID NO: 16. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-62668 and consists of the polypeptide sequence of SEQ ID NO: 17. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-13395 and consists of the polypeptide sequence of SEQ ID NO: 18. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus horneckiae and consists of the polypeptide sequence of SEQ ID NO: 19. In a preferred embodiment of the present invention, the DNase is obtained from Bacillus sp-11238 and consists of the polypeptide sequence of SEQ ID NO:20.In a preferred embodiment of the invention, the DNase is obtained from Bacillus cibi and consists of the polypeptide sequence of SEQ ID NO: 21. In a preferred embodiment of the invention, the DNase is obtained from Bacillus sp-18318 and consists of the polypeptide sequence of SEQ ID NO: 22. In a preferred embodiment of the invention, the DNase is obtained from Bacillus idriensis and consists of the polypeptide sequence of SEQ ID NO: 23.

[0297] Biofilms can form on fabrics when microorganisms are present on an item and adhere to each other on the item. Some microorganisms tend to adhere to the surface of items such as fabrics. Some microorganisms adhere to such surfaces and form biofilms on the surface. Because biofilms are considered to be sticky, the attached microorganisms and / or biofilms can be difficult to remove. Furthermore, biofilms adhere to soils due to the stickiness of the biofilms. Commercially available laundry detergent compositions do not remove such microorganisms or biofilms.

[0298] The present invention relates to polypeptides with DNase activity and the use of such polypeptides to prevent, reduce, or remove biofilms from items such as fabrics. In one embodiment of the present invention, polypeptides with DNase activity are used to prevent, reduce, or remove stickiness from items. In one embodiment of the present invention, polypeptides with DNase activity improve the whiteness of items such as fabrics. In one embodiment, polypeptides of the present invention with DNase activity help maintain the color of fabrics. Repeated washing of fabrics tends to cause their color to lose brightness. In one embodiment, polypeptides of the present invention with DNase activity have improved effectiveness in maintaining the color of colored fabrics, even after repeated washing. In one embodiment, the polypeptides of the present invention also reduced the coloring of uncolored portions of the same or another fabric present in the wash liquor.

[0299] Polypeptides with DNase activity can also be used to pre-treat soils on fabrics, such as fabrics that have a significant amount of biofilm attached.

[0300] Polypeptides with DNase activity can also be used to prevent, reduce or remove static electricity from items that may accumulate static electricity, such as fabrics or hard surfaces. Polypeptides with DNase activity can also be used to prevent, reduce and / or remove biofilms from items, such as hard surfaces such as dishes, cutlery, porcelain, ceramics, crockery, etc. Thus, in some embodiments, polypeptides with DNase activity can be used in ADW (automatic dishwashing) processes.

[0301] Additionally, the present invention relates to the use of polypeptides with DNase activity to prevent, reduce or remove redeposition of soil during a wash cycle, for example, when the polypeptides are used for laundering fabrics, the polypeptides prevent soils present in the wash liquor from adhering to the fabrics.

[0302] Furthermore, the present invention relates to the use of a polypeptide having DNase activity for preventing, reducing or removing stain adhesion from an item. In one embodiment, the item is a fabric. If stains do not adhere to the item, the item will appear cleaner. Therefore, the present invention also relates to the use of a polypeptide having DNase activity for maintaining or improving the whiteness of an item.

[0303] When items such as T-shirts or sportswear are used, they are exposed to bacteria from the user's body and other environments in which they are used. This can cause the items to malodor, even after they have been washed. The present invention relates to the removal or reduction of malodors on fabrics. The malodor can be caused by bacteria that produce compounds with unpleasant odors. One example of such an unpleasant-smelling compound is E-2-nonenal. The malodor can be present on freshly washed fabrics that are still wet. Alternatively, the malodor can be present on freshly washed fabrics that have been dried. The malodor can also be present on fabrics that have been stored for a period of time after washing. The present invention also relates to the reduction or removal of malodors, such as E-2-nonenal, from wet or dry fabrics.

[0304] The polypeptide of the present invention having DNase activity, i.e., the DNase of the present invention, can be prepared by the following steps: and have very good cleaning performance in liquid detergents. DNases of SEQ ID NOs: 8, 9 and 10, as well as homologous DNases, such as those having DNase activity and the following: SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 53, SEQ ID NO: 56, SEQ ID NO: 59, SEQ ID NO: 62, SEQ ID NO: 65, SEQ ID NO: 68, SEQ ID NO: 71, SEQ ID NO: 74, SEQ ID NO: 77, SEQ ID NO: 80, SEQ ID NO: 83, SEQ ID NO: 86, SEQ ID NO: 89, SEQ ID NO: 92, SEQ ID NO: 95, SEQ ID NO: 98, SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 1 Examples of beneficial effects of polypeptides having the polypeptide sequences set forth in SEQ ID NOs: 07, 110, 113, 116, 119, 122, 125, 128, 131, 134, 137, 140, 143, 146, 149, 152, 155, 158, 161, 164, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, and 197 are shown. Deep cleaning effects are shown in Examples 2 and 3, including laundry graying prevention and odor elimination. Polypeptides including SEQ ID NOs: 8, 9, and 10 are novel polypeptides with DNase activity that have deep cleaning effects in powder and liquid detergents.SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116, SEQ ID NO:119 , SEQ ID NO:122, SEQ ID NO:125, SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:158, SEQ ID NO:161, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:170, SEQ ID NO:176, SEQ ID NO:179, SEQ ID NO:185, SEQ ID NO:188, SEQ ID NO:191 and SEQ ID NO:197 are also polypeptides having DNase activity that have deep cleaning effects in powder detergents and liquid detergents.

[0305] Benzonase (SIGMA-E1014) SEQ ID NO: 7) is a commercially available DNase. We demonstrate that this DNase also has a deep cleaning effect, as seen in Example 2. The deep cleaning effect prevents laundry from graying and helps remove laundry odors.

[0306] Another embodiment relates to the use of a DNase selected from the group consisting of SEQ ID NOs: 7, 8, 9 and 10 for reducing malodours from laundry and / or fabrics. Another embodiment relates to the use of a DNase selected from the group consisting of SEQ ID NOs: 7, 8, 9 and 10 for reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving fabric whiteness.

[0307] One aspect of the invention relates to the use of a polypeptide with DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide with DNase activity comprises an HXXP motif, where H is the amino acid histidine, P is the amino acid proline, and X is any amino acid. The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0308] One aspect of the invention relates to the use of a polypeptide with DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide with DNase activity comprises one or more of the motifs: [E / D / H]H[I / V / L / F / M]X[P / A / S], [T / D / S][G / N]PQL, [G / T]Y[D / S][R / K / L]. The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0309] One aspect of the invention relates to the use of a polypeptide with DNase activity for preventing, reducing or removing biofilm from an item, wherein the polypeptide with DNase activity comprises one or both of the motifs: [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R]. The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0310] One aspect of the invention relates to the use of a polypeptide with DNase activity for preventing, reducing or removing biofilm from an item, wherein the polypeptide with DNase activity belongs to the GYS clade and comprises one or both of the motifs: [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205). The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0311] An aspect of the invention relates to the use of a polypeptide having DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide having DNase activity belongs to the GYS clade and comprises a polypeptide selected from the following: SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77 and SEQ ID NO:80, or a polypeptide having at least 80%, such as at least 85%, such as at least 90%, such as at least 95% or 100% sequence identity thereto. The item is preferably a textile, such as a fabric, for example cotton and / or polyester.

[0312] One aspect of the present invention relates to the use of a polypeptide having DNase activity for preventing, reducing or removing biofilm from an item, wherein the polypeptide having DNase activity belongs to the NAWK clade and comprises one or both of the motifs: [V / I]PL[S / A]NAWK (SEQ ID NO: 206) or NPQL (SEQ ID NO: 207). The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0313] An aspect of the invention relates to the use of a polypeptide having DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide having DNase activity belongs to the NAWK clade and comprises a polypeptide selected from the following: SEQ ID NO: 83, SEQ ID NO: 86, SEQ ID NO: 89, SEQ ID NO: 92, SEQ ID NO: 95, SEQ ID NO: 98, SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 107, SEQ ID NO: 110, SEQ ID NO: 113, SEQ ID NO: 116 and SEQ ID NO: 119, or a polypeptide having at least 80%, such as at least 85%, such as at least 90%, such as at least 95% or 100% sequence identity thereto. The item is preferably a textile, such as a fabric, for example cotton and / or polyester.

[0314] One aspect of the invention relates to the use of a polypeptide with DNase activity for preventing, reducing or removing biofilm from an item, wherein the polypeptide with DNase activity belongs to the KNAW clade and comprises one or both of the motifs: P[Q / E]L[W / Y] (SEQ ID NO: 208) or [K / H / E]NAW (SEQ ID NO: 209). The item is preferably a textile, e.g., a fabric, such as cotton and / or polyester.

[0315] An aspect of the invention relates to the use of a polypeptide having DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide having DNase activity belongs to the KNAW clade and comprises a polypeptide selected from the following: SEQ ID NO: 122, SEQ ID NO: 125, SEQ ID NO: 128, SEQ ID NO: 131, SEQ ID NO: 134, SEQ ID NO: 137, SEQ ID NO: 140, SEQ ID NO: 143, SEQ ID NO: 146, SEQ ID NO: 149, SEQ ID NO: 152, SEQ ID NO: 155 and SEQ ID NO: 158, or a polypeptide having at least 80%, such as at least 85%, for example at least 90%, such as at least 95% or 100% sequence identity thereto. The item is preferably a textile, such as a fabric, for example cotton and / or polyester.

[0316] An aspect of the invention relates to the use of a polypeptide having DNase activity for the prevention, reduction or removal of biofilm from an item, wherein the polypeptide having DNase activity comprises a polypeptide selected from the polypeptides set forth in SEQ ID NO: 161, SEQ ID NO: 164, SEQ ID NO: 167, SEQ ID NO: 170, SEQ ID NO: 173, SEQ ID NO: 176, SEQ ID NO: 179, SEQ ID NO: 182, SEQ ID NO: 185, SEQ ID NO: 188, SEQ ID NO: 191, SEQ ID NO: 194 and SEQ ID NO: 197, or a polypeptide having at least 80%, such as at least 85%, such as at least 90%, such as at least 95% or 100% sequence identity thereto. The item is preferably a textile, such as a fabric, for example cotton and / or polyester.

[0317] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 11 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0318] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 12, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0319] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 13, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0320] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 14, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0321] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 15, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0322] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 16, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0323] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 17, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0324] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 18, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0325] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 19, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0326] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 20, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0327] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 21 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0328] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 22, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0329] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 23, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0330] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 53, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0331] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 56, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0332] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 59, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0333] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 62, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0334] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 65, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0335] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 68, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0336] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 71 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0337] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 74, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0338] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 77, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0339] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 80, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0340] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 83, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0341] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 86, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0342] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 89, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0343] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 92 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0344] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 95 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0345] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 98 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0346] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 101, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0347] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 104, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0348] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 107, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0349] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 110, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0350] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 113, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0351] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 116, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0352] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 119, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0353] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 122 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0354] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 125, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0355] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 128, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0356] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 131 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving fabric whiteness.

[0357] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 134, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0358] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 137, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0359] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 140, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0360] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 143, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0361] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 146 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0362] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 149 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0363] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 152, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0364] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 155, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0365] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 158, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0366] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 161, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0367] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 164, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0368] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 167, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0369] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 170, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0370] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 173, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0371] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 176 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0372] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 179, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0373] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 182, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0374] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 185, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0375] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 188, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0376] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 191 or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0377] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 194, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0378] One aspect of the present invention relates to the use of a polypeptide with DNase activity, comprising the amino acid sequence set forth in SEQ ID NO: 197, or a polypeptide having at least 80% sequence identity thereto, for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0379] A particularly preferred embodiment of the present invention relates to a DNase derived from Bacillus, such as a Bacillus DNase, preferably Bacillus sp. sp-62451, or a closely related DNase thereof having at least 80% sequence identity to the polypeptide having the amino acid sequence set forth in SEQ ID NO: 8 (Bacillus sp. sp-62451), such as a DNase selected from the group consisting of Bacillus cibi, Bacillus sp-18318 and Bacillus idriensis. A preferred embodiment of the present invention relates to the use of a DNase obtained from a Bacillus, such as Bacillus sp. sp-62451, comprising a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 8.A preferred embodiment of the present invention relates to the use of a DNase obtained from a Bacillus species, such as Bacillus cibi, comprising a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 21 for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics. A preferred embodiment of the present invention relates to the use of a DNase obtained from Bacillus, such as Bacillus sp-18318, comprising a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 22 for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving fabric whiteness.A preferred embodiment of the present invention relates to the use of a DNase obtained from the genus Bacillus, such as Bacillus idriensis, comprising a polypeptide having at least 60%, such as at least 65%, at least 70%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 23 for the purpose of reducing malodours from laundry and / or fabrics, preventing redeposition and maintaining or improving the whiteness of fabrics.

[0380] As mentioned above, the DNase polypeptides of the present invention have particular deep cleaning power, for example, the DNases of the present invention are particularly effective in disrupting or removing biofilms or components of biofilms such as polysaccharides, proteins, DNA, dirt, or other components present in biofilms. Thus, the DNase polypeptides of the present invention are particularly effective in preventing, reducing or removing biofilms from items such as fabrics and hard surfaces.

[0381] The polypeptide having DNase activity is preferably obtained from a Bacillus sp. or Paenibacillus. The present invention relates to a polypeptide having at least 60% sequence identity to any of the mature polypeptides of SEQ ID NO: 2, 4 or 6 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item. The present invention further relates to a polypeptide having at least 60%, such as 70%, such as 80%, or at least 90% sequence identity to any of the mature polypeptides of SEQ ID NO: 2, 4 or 6 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item. The present invention further relates to a polypeptide having at least 60%, such as 70%, such as 80%, or at least 90% sequence identity to any of the polypeptides of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22 or SEQ ID NO:23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0382] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0383] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0384] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0385] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0386] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0387] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0388] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0389] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0390] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0391] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0392] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0393] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0394] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0395] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0396] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0397] In one embodiment, the present invention relates to a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0398] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0399] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0400] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0401] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0402] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0403] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0404] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0405] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0406] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0407] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0408] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0409] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0410] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0411] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0412] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0413] In one embodiment, the present invention relates to a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0414] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0415] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0416] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0417] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0418] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0419] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0420] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0421] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0422] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0423] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0424] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0425] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0426] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0427] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0428] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0429] In one embodiment, the present invention relates to a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0430] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0431] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0432] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0433] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0434] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0435] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0436] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0437] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0438] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0439] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0440] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0441] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0442] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0443] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0444] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0445] In one embodiment, the present invention relates to a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0446] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0447] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0448] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0449] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0450] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0451] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0452] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0453] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0454] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0455] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0456] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0457] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0458] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0459] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0460] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0461] In one embodiment, the present invention relates to a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0462] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 8 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0463] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 9 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0464] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 10 and having DNase activity, wherein the polypeptide is used to prevent, reduce or remove biofilm from an item.

[0465] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 11 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0466] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 12 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0467] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 13 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0468] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 14 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0469] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 15 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0470] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 16 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0471] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 17 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0472] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 18 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0473] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 19 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0474] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 20 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0475] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 21 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0476] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 22 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0477] In one embodiment, the present invention relates to a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 23 and having DNase activity, wherein the polypeptide can be used to prevent, reduce or remove biofilm from an item.

[0478] Preferred polypeptides of the invention are DNases from Bacillus sp-62451 (mature polypeptide of SEQ ID NO: 8 or SEQ ID NO: 2) and closely related polypeptides having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 8. Also claimed homologous polypeptides are Bacillus cibi, SEQ ID NO: 21, Bacillus sp-18318, SEQ ID NO: 22, and Bacillus idriensis, SEQ ID NO: 23, and DNases having at least 80% sequence identity thereto.

[0479] Preferred polypeptides of the present invention are DNase derived from Bacillus horikoshii (SEQ ID NO: 9, or the mature polypeptide of SEQ ID NO: 4) and closely related polypeptides having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 9. Also claimed are homologous polypeptides of Bacillus sp-62520 SEQ ID NO:11, Bacillus sp-62520 SEQ ID NO:12, Bacillus horikoshii SEQ ID NO:13, Bacillus horikoshii SEQ ID NO:14, Bacillus sp-16840 SEQ ID NO:15, Bacillus sp-16840 SEQ ID NO:16, Bacillus sp-62668 SEQ ID NO:17, Bacillus sp-13395 SEQ ID NO:18, Bacillus horneckiae SEQ ID NO:19, Bacillus sp-11238 SEQ ID NO:20, and DNases having at least 80% sequence identity thereto.

[0480] Preferred polypeptides of the present invention are DNase derived from Paenibacillus sp.-18057 (SEQ ID NO: 10, or the mature polypeptide of SEQ ID NO: 6) and closely related polypeptides having at least 80% sequence identity with the mature polypeptide of SEQ ID NO: 10.

[0481] The deep cleaning effect of the polypeptides of SEQ ID NOs: 8, 9 and 10 having DNase activity, and homologous polypeptides having at least 80% identity to SEQ ID NOs: 8, 9 and 10, is shown in Example 2.

[0482] The term "deep cleaning" refers to the disruption or removal of biofilms or components thereof such as polysaccharides, proteins, DNA, dirt or other components present in biofilms.

[0483] The polypeptides of the present invention preferably comprise or consist of the amino acid sequence set forth in SEQ ID NO:8, 9 or 10 or any of their allelic variants; or are fragments thereof having DNase activity. In another embodiment, the polypeptides comprise or consist of the mature form of any of the polypeptides having the amino acid sequence set forth in SEQ ID NO:2, 4 or 6. The polypeptides of the present invention preferably comprise the mature form of any of the following: SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116, SEQ ID NO:119, SEQ ID NO: or a fragment thereof that has DNase activity.In another aspect, the polypeptide is selected from the group consisting of: SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116, The polypeptide may comprise or consist of a mature form of any of the polypeptides having the amino acid sequence set forth in SEQ ID NO:119, SEQ ID NO:122, SEQ ID NO:125, SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:155, SEQ ID NO:158, SEQ ID NO:161, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:170, SEQ ID NO:173, SEQ ID NO:176, SEQ ID NO:179, SEQ ID NO:182, SEQ ID NO:185, SEQ ID NO:188, SEQ ID NO:191, SEQ ID NO:194 or SEQ ID NO:197.

[0484] In one embodiment, the polypeptide is isolated.

[0485] In another embodiment, the invention provides a polypeptide of the mature form of SEQ ID NO:2, 4 or 6 or the following: SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:53, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:101, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:110, SEQ ID NO:113, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118, SEQ ID NO:119, SEQ ID NO:220, SEQ ID NO:221, SEQ ID NO:222, SEQ ID NO:233, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:59, SEQ ID NO:62, SEQ ID NO:65, SEQ ID NO:68, SEQ ID NO:71, SEQ ID NO:74, SEQ ID NO:77, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:86, SEQ ID NO:89, SEQ ID NO:92, SEQ ID NO:95, SEQ ID NO

[0033] The present invention relates to variants of any of the homologous polypeptides having the amino acid sequence set forth in SEQ ID NO:119, SEQ ID NO:122, SEQ ID NO:125, SEQ ID NO:128, SEQ ID NO:131, SEQ ID NO:134, SEQ ID NO:137, SEQ ID NO:140, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:155, SEQ ID NO:158, SEQ ID NO:161, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:170, SEQ ID NO:173, SEQ ID NO:176, SEQ ID NO:179, SEQ ID NO:182, SEQ ID NO:185, SEQ ID NO:188, SEQ ID NO:191, SEQ ID NO:194 or SEQ ID NO:197, which contain substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the mature polypeptide of SEQ ID NO:2, 4 or 6 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0486] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 8 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 8 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0487] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 9 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 9 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0488] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 10 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 10 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0489] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 11 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 11 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0490] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 12 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 12 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0491] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 13 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 13 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0492] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 14 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 14 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0493] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 15 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 15 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0494] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 16 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 16 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0495] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 17 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 17 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0496] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 18 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 18 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0497] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 19 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 19 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0498] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 20 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 20 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0499] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 21 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 21 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0500] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 22 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 22 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0501] In another embodiment, the invention relates to variants of the mature polypeptide of SEQ ID NO: 23 comprising substitutions, deletions, and / or insertions at one or more (e.g., several) positions. In one embodiment, the number of amino acid substitutions, deletions, and / or insertions introduced into the polypeptide of SEQ ID NO: 23 is 10 or less, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.

[0502] The amino acid mutations may be of a minor nature, i.e., conservative amino acid substitutions or insertions that do not significantly affect the folding and / or activity of the protein; small deletions, typically of 1 to 30 amino acids; small amino- or carboxyl-terminal extensions such as an amino-terminal methionine residue; small linker peptides of 20 to 25 residues or less; or small extensions that facilitate purification by altering the net charge or other function, such as a poly-histidine sequence, an antigenic epitope, or a binding domain.

[0503] Examples of conservative substitutions are within the groups of basic amino acids (arginine, lysine, and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine, and valine), aromatic amino acids (phenylalanine, tryptophan, and tyrosine), and small amino acids (glycine, alanine, serine, threonine, and methionine). Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, in H. Neurath and R.L. Hill, 1979, In, The Proteins, Academic Press, New York. Frequently occurring substitutions are Ala / Ser, Val / Ile, Asp / Glu, Thr / Ser, Ala / Gly, Ala / Thr, Ser / Asn, Ala / Val, Ser / Gly, Tyr / Phe, Ala / Pro, Lys / Arg, Asp / Asn, Leu / Ile, Leu / Val, Ala / Glu, and Asp / Gly.

[0504] Alternatively, the amino acid mutations may be of such a nature that they alter the physicochemical properties of the polypeptide, for example, they may improve the thermostability of the polypeptide, alter its substrate specificity, or change its pH optimum.

[0505] Essential amino acids in a polypeptide can be identified according to techniques known in the art, such as site-directed mutagenesis or alanine scanning mutagenesis (Cunningham and Wells, 1989, Science 244:1081-1085). In the latter technique, single alanine mutations are introduced at every residue in the molecule, and the resulting mutant molecules are tested for DNase activity to identify amino acid residues important for the molecule's activity. See also Hilton et al., 1996, J. Biol. Chem. 271:4699-4708. Enzyme active sites or other biological interactions can also be determined by physical analysis of the structure, as determined by techniques such as nuclear magnetic resonance, crystallography, electron diffraction, or photoaffinity labeling, coupled with mutations of putative contact site amino acids. See, for example, de Vos et al., 1992, Science 255:306-312; Smith et al., 1992, J. Mol. Biol. 224:899-904; Wlodaver et al., 1992, FEBS Lett. 309:59-64. Use of a polypeptide having DNase activity 3: 568-576; Svetina et al., 2000, J. Biotechnol. 76:245-251; Rasmussen-Wilson et al., 1997, Appl. Environ. Microbiol. 63:3488-3493; Ward et al., 1995, Biotechnology 13:498-503; and Contreras et al., 1991, Biotechnology 9:378-381; Eaton et al. al., 1986, Biochemistry 25:505-512; Collins-Racie et al., 1995, Biotechnology 13:982-987; Carter et al., 1989, Proteins: Structure, Function, and Genetics 6:240-248; and Stevens, 2003, Drug Discovery World 4:35-48.

[0506] Polynucleotides The present invention also relates to polynucleotides encoding the polypeptides described herein. In one embodiment, the polynucleotides encoding the polypeptides of the present invention are isolated. In another embodiment, the polynucleotides encoding the polypeptides of the present invention comprise or consist of the polynucleotide sequence set forth in SEQ ID NO: 1, 3, or 5.

[0507] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under low stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5, or (ii) the full-length complement of (i) (Sambrook, et al., 1989, Molecular Cloning, A Laboratory Manual, 2nd edition, Cold Spring Harbor, New York). In one embodiment, the polypeptide is isolated.

[0508] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under low-to-moderate stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide is isolated.

[0509] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under moderate stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide is isolated.

[0510] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under moderate to high stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide is isolated.

[0511] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under high stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide is isolated.

[0512] In another embodiment, the invention relates to an isolated polypeptide having DNase activity encoded by a polynucleotide that hybridizes under very high stringency conditions to (i) the mature polypeptide coding sequence of SEQ ID NO: 1, 3 or 5, or (ii) the full-length complement of (i). In one embodiment, the polypeptide is isolated.

[0513] Using the polynucleotide of SEQ ID NO: 1, 3, or 5, or a subsequence thereof, and the polypeptide of SEQ ID NO: 2, 4, or 6, or a fragment thereof, or the polypeptide of SEQ ID NO: 8, 9, or 10, or a fragment thereof, nucleic acid probes can be designed for identifying and cloning DNA encoding polypeptides having DNase activity from strains of different genera or species according to methods well known in the art. In particular, such probes can be used for hybridization with genomic DNA of cells of interest to identify and isolate the corresponding gene contained therein, following standard Southern blotting procedures. Such probes can be significantly shorter than the entire sequence, but should be at least 15 nucleotides in length, e.g., at least 25, at least 35, or at least 70 nucleotides in length. Preferably, the nucleic acid probe is at least 100 nucleotides in length, e.g., at least 200 nucleotides, at least 300 nucleotides, at least 400 nucleotides, at least 500 nucleotides, or at least 600 nucleotides in length. Both DNA and RNA probes can be used. The probes are typically labeled to detect the corresponding gene (e.g., 32 P, 3 H, 35 S, biotin, or avidin). Such probes are encompassed by the present invention.

[0514] Genomic DNA or cDNA (if the polypeptide contains an intron) libraries prepared from such other strains may be screened for DNA that hybridizes with the above-described probe and encodes a polypeptide having DNase activity. Genomic or other DNA from such other strains may be separated by agarose or polyacrylamide gel electrophoresis or other separation techniques. DNA from these libraries or separated DNA may be transferred to and immobilized on nitrocellulose or other suitable carrier material. The carrier material is used in Southern blots to identify clones or DNA that hybridize to SEQ ID NO: 1, 3, or 5, or a subsequence thereof.

[0515] For purposes of this invention, hybridization means hybridization of a polynucleotide to a labeled nucleic acid probe corresponding to (i) SEQ ID NO: 1, 3, or 5; (ii) the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5; (iii) a full-length complement thereof; or (iv) a subsequence thereof, under very low stringency, low stringency, low-moderate stringency, medium stringency, medium-high stringency, high stringency, or very high stringency conditions. Molecules to which the nucleic acid probe hybridizes under these conditions can be detected, for example, using X-ray film or any other detection means known in the art.

[0516] In another embodiment, the invention relates to a polypeptide having DNase activity encoded by a polynucleotide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, 3, or 5. In another embodiment, the polypeptide is isolated.

[0517] In another embodiment, the present invention provides SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:54, SEQ ID NO:57, SEQ ID NO:60, SEQ ID NO:63, SEQ ID NO:66, SEQ ID NO:69, SEQ ID NO:72, SEQ ID NO:75, SEQ ID NO:78, SEQ ID NO:81, SEQ ID NO:84, SEQ ID NO:87, SEQ ID NO:90, SEQ ID NO:93, SEQ ID NO:96, SEQ ID NO:99, SEQ ID NO:102, SEQ ID NO:105, SEQ ID NO:108, SEQ ID NO:111, SEQ ID NO:114, SEQ ID NO:117, SEQ ID NO:120, SEQ ID NO:123, SEQ ID NO:126, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:135, SEQ ID NO:138, SEQ ID NO:141, SEQ ID NO:144, SEQ ID NO:147, The present invention relates to a polypeptide having DNase activity encoded by a polynucleotide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 150, SEQ ID NO: 153, SEQ ID NO: 156, SEQ ID NO: 159, SEQ ID NO: 162, SEQ ID NO: 165, SEQ ID NO: 168, SEQ ID NO: 171, SEQ ID NO: 174, SEQ ID NO: 177, SEQ ID NO: 180, SEQ ID NO: 183, SEQ ID NO: 186, SEQ ID NO: 189, SEQ ID NO: 192, SEQ ID NO: 195. In another embodiment, the polypeptide is isolated.

[0518] Techniques used to isolate or clone polynucleotides are known in the art and include isolation from genomic DNA, or a combination thereof. Cloning of polynucleotides from genomic DNA can be performed, for example, by detecting cloned DNA fragments with shared structural features using the well-known polymerase chain reaction (PCR) or antibody screening of expression libraries. See, for example, Innis et al., 1990, PCR: A Guide to Methods and Applications, Academic Press, New York. Other nucleic acid amplification methods, such as ligase chain reaction (LCR), ligation-activated transcription (LAT), and polynucleotide-based amplification (NASBA), can also be used.

[0519] Modification of polynucleotides encoding polypeptides of the invention may be necessary to synthesize polypeptides substantially similar to the polypeptides. The term "substantially similar" to a polypeptide refers to non-naturally occurring forms of the polypeptide. These polypeptides may differ in some engineered way from the polypeptide isolated from its natural source, e.g., variants that differ in specific activity, thermostability, pH optimum, etc. Variants can be constructed based on the polynucleotides represented as the mature polypeptide coding sequences of SEQ ID NOs: 1, 3, or 5, or subsequences thereof, and / or by introducing nucleotide substitutions that do not result in a change in the amino acid sequence of the polypeptide but correspond to the codon usage of the intended host organism for production of the enzyme, or that may result in a different amino acid sequence. For a general review of nucleotide substitutions, see, e.g., Ford et al., 1991, Protein Expression and Purification 2:95-107.

[0520] nucleic acid construct The present invention also relates to nucleic acid constructs comprising a polynucleotide of the invention operably linked to one or more control sequences that provide for the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences.

[0521] Polynucleotides can be manipulated in a variety of ways to result in expression of a polypeptide. Manipulation of the polynucleotide prior to insertion into a vector may be desirable or necessary, depending on the expression vector. Techniques for modifying polynucleotides utilizing recombinant DNA methods are well known in the art.

[0522] The control sequence may be a promoter, which is a polynucleotide recognized by a host cell for expression of a polynucleotide encoding a polypeptide of the present invention. The promoter contains transcriptional control sequences that mediate expression of the polypeptide. The promoter may be any polynucleotide that exhibits transcriptional activity in the host cell, including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides that are homologous or heterologous to the host cell.

[0523] Examples of suitable promoters for directing transcription of the nucleic acid constructs of the invention in bacterial host cells include the Bacillus amyloliquefaciens α-amylase gene (amyQ), the Bacillus licheniformis α-amylase gene (amyL), the Bacillus licheniformis penicillinase gene (penP), the Bacillus stearothermophilus maltogenic amylase gene (amyM), the Bacillus subtilis levansucrase gene (sacB), the Bacillus subtilis xylA and xylB genes, and the Bacillus thuringiensis cryIIIA gene (Agaisse and Lereclus, 1994, Molecular Microbiology 13:97-107), the E. coli lac operon, the E. coli trc promoter (Egon et al., 1988, Gene 69:301-315), the Streptomyces coelicolor agarase gene (dagA) and the prokaryotic β-lactamase gene (Villa-Kamaroff et al., 1978, Proc. Natl. Acad. Sci. USA 75:3727-3731), and the tac promoter (DeBoer et al., 1983, Proc. Natl. Acad. Sci. USA 80:21-25). Further promoters are described in "Useful proteins from recombinant bacteria," Gilbert et al., 1980, Scientific American 242:74-94; and Sambrook et al., supra, 1989. Examples of tandem promoters are disclosed in WO 99 / 42835.

[0524] Examples of suitable promoters for directing the transcription of the nucleic acid constructs of the invention in a filamentous fungal host cell include those encoding Aspergillus nidulans acetamidase, Aspergillus niger neutral α-amylase, Aspergillus niger acid-stable α-amylase, Aspergillus niger or Aspergillus awamori glucoamylase (glaA), Aspergillus oryzae TAKA amylase, Aspergillus oryzae alkaline protease, Aspergillus oryzae triose phosphate isomerase, Fusarium oxysporum fumarate, and the like. oxysporum trypsin-like protease (WO 96 / 00787), Fusarium venenatum amyloglucosidase (WO 00 / 56900), Fusarium venenatum Daria (WO 00 / 56900), Fusarium venenatum Quinn (WO 00 / 56900), Rhizomucor miehei lipase, Rhizomucor miehei aspartic proteinase, Trichoderma reesei β-glucosidase, Trichoderma reesei reesei cellobiohydrolase I, Trichoderma reesei cellobiohydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reeseiTrichoderma reesei endoglucanase III, Trichoderma reesei endoglucanase V, Trichoderma reesei xylanase I, Trichoderma reesei xylanase II, Trichoderma reesei xylanase III, Trichoderma reesei β-xylosidase, and Trichoderma reesei translation elongation factor, as well as the NA2-tpi promoter (a modified promoter from the Aspergillus neutral α-amylase gene in which the non-translated leader has been replaced with the non-translated leader from the Aspergillus triosephosphate isomerase gene; a non-limiting example is the NA2-tpi promoter in which the non-translated leader has been replaced with the non-translated leader from the Aspergillus nidulans gene). Examples of promoters include those derived from genes of Aspergillus niger (including a modified promoter from the Aspergillus niger neutral α-amylase gene in which the non-translated leader from the Aspergillus nidulans or Aspergillus oryzae triosephosphate isomerase gene has been substituted); as well as mutant, truncated, and hybrid promoters thereof. Other promoters are described in U.S. Patent No. 6,011,147.

[0525] In yeast hosts, useful promoters are obtained from the genes for Saccharomyces cerevisiae enolase (ENO-1), Saccharomyces cerevisiae galactokinase (GAL1), Saccharomyces cerevisiae alcohol dehydrogenase / glyceraldehyde-3-phosphate dehydrogenase (ADH1, ADH2 / GAP), Saccharomyces cerevisiae triosephosphate isomerase (TPI), Saccharomyces cerevisiae metallothionein (CUP1), and Saccharomyces cerevisiae 3-phosphoglycerate kinase. Other useful promoters for yeast host cells are described by Romanos et al., 1992, Yeast 8:423-488.

[0526] The control sequence may also be a transcription terminator recognized by a host cell to terminate transcription. The terminator is operably linked to the 3'-end of the polynucleotide encoding the polypeptide. Any terminator that functions in the host cell may be used in the present invention.

[0527] Preferred terminators for bacterial host cells are obtained from the Bacillus clausii alkaline protease (aprH), the Bacillus licheniformis alpha-amylase gene (amyL), and the Escherichia coli ribosomal RNA (rrnB) gene.

[0528] Preferred terminators for filamentous fungal host cells include Aspergillus nidulans acetamidase, Aspergillus nidulans anthranilate synthase, Aspergillus niger glucoamylase, Aspergillus niger α-glucosidase, Aspergillus oryzae TAKA amylase, Fusarium oxysporum trypsin-like protease, Trichoderma reesei β-glucosidase, Trichoderma reesei cellobiohydrolase I, Trichoderma reesei β-glucosidase ... reesei cellobiohydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reesei endoglucanase III, Trichoderma reesei endoglucanase V, Trichoderma reesei xylanase I, Trichoderma reesei xylanase II, Trichoderma reesei xylanase III, Trichoderma reesei β-xylosidase, and Trichoderma reesei translation elongation factor genes.

[0529] Preferred terminators for yeast host cells are obtained from the genes for Saccharomyces cerevisiae enolase, Saccharomyces cerevisiae cytochrome C (CYC1), and Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase. Other useful terminators for yeast host cells are described in Romanos et al., 1992, supra.

[0530] The regulatory sequence may also be an mRNA stabilizing region downstream of the promoter and upstream of the coding sequence of the gene, which increases expression of the gene.

[0531] Examples of suitable mRNA stabilization regions are obtained from the Bacillus thuringiensis cryIIIA gene (WO 94 / 25612) and the Bacillus subtilis SP82 gene (Hue et al., 1995, Journal of Bacteriology 177:3465-3471).

[0532] The control sequence may also be a leader, i.e., a non-translated region of an mRNA, which is important for translation by the host cell. The leader is operably linked to the 5'-terminus of the polynucleotide encoding the polypeptide. Any leader that is functional in the host cell may be used.

[0533] Preferred leaders for filamentous fungal host cells are obtained from the genes for Aspergillus oryzae TAKA amylase and Aspergillus nidulans triose phosphate isomerase.

[0534] Suitable leaders for yeast host cells are obtained from the genes for Saccharomyces cerevisiae enolase (ENO-1), Saccharomyces cerevisiae 3-phosphoglycerate kinase, Saccharomyces cerevisiae α-factor, and Saccharomyces cerevisiae alcohol dehydrogenase / glyceraldehyde-3-phosphate dehydrogenase (ADH2 / GAP).

[0535] The control sequence may also be a polyadenylation sequence, which is a sequence operably linked to the 3'-terminus of a polynucleotide that, when transcribed, is recognized by a host cell as a signal for adding polyadenosine residues to the transcribed mRNA. Any polyadenylation sequence that functions in the host cell may be used.

[0536] Preferred polyadenylation sequences for filamentous fungal host cells are obtained from the genes for Aspergillus nidulans anthranilate synthase, Aspergillus niger glucoamylase, Aspergillus niger α-glucosidase, Aspergillus oryzae TAKA amylase, and Fusarium oxysporum trypsin-like protease.

[0537] Useful polyadenylation sequences for yeast host cells are described in Guo and Sherman, 1995, Mol. Cellular Biol. 15:5983-5990.

[0538] A control sequence may also be a signal peptide coding region that encodes a signal peptide linked to the N-terminus of a polypeptide and directs the polypeptide into the secretory pathway of a cell. The 5'-end of a polynucleotide coding sequence may inherently contain a signal peptide coding sequence originally linked in translation reading frame with the segment of the coding sequence encoding the polypeptide. Alternatively, the 5'-end of the coding sequence may contain a signal peptide coding sequence foreign to the coding sequence. A foreign signal peptide coding sequence may be required when the coding sequence does not naturally contain a signal peptide coding sequence. Alternatively, the foreign signal peptide coding sequence may simply replace a native signal peptide coding sequence to enhance secretion of the polypeptide. However, any signal peptide coding sequence that directs an expressed polypeptide into the secretory pathway of a host cell may be used.

[0539] Effective signal peptide coding sequences for bacterial host cells are those obtained from the genes for Bacillus NCIB11837 maltogenic amylase, Bacillus licheniformis subtilisin, Bacillus licheniformis β-lactamase, Bacillus stearothermophilus α-amylase, Bacillus stearothermophilus neutral protease (nprT, nprS, nprM), and Bacillus subtilis prsA. Additional signal peptides are described in Simonen and Palva, 1993, Microbiological Reviews 57:109-137.

[0540] Effective signal peptide coding sequences for filamentous fungal host cells are those obtained from the genes for Aspergillus niger neutral amylase, Aspergillus niger glucoamylase, Aspergillus oryzae TAKA amylase, Humicola insolens cellulase, Humicola insolens endoglucanase V, Humicola lanuginosa lipase, and Rhizomucor miehei aspartic proteinase.

[0541] Useful signal peptides for yeast host cells are obtained from the genes for Saccharomyces cerevisiae α-factor and Saccharomyces cerevisiae invertase. Other useful signal peptide coding sequences are described by Romanos et al., 1992, supra.

[0542] The control sequence may also be a propeptide coding sequence, which encodes a propeptide position at the N-terminus of a polypeptide. The resulting polypeptide is known as a zymogen or propolypeptide (or, in some cases, a zymogen). Propolypeptides are generally inactive and can be converted to active polypeptides by catalytic or autocatalytic cleavage of the propeptide from the propolypeptide. Propeptide coding sequences can be obtained from the genes for Bacillus subtilis alkaline protease (aprE), Bacillus subtilis neutral protease (nprT), Myceliophthora thermophila laccase (WO 95 / 33836), Rhizomucor miehei aspartic proteinase, and Saccharomyces cerevisiae α-factor.

[0543] When both a signal peptide and a propeptide sequence are present, the propeptide sequence is positioned adjacent to the N-terminus of the polypeptide, and the signal peptide sequence is positioned adjacent to the N-terminus of the propeptide sequence.

[0544] It may also be desirable to add regulatory sequences that regulate polypeptide expression relative to host cell growth. Examples of regulatory sequences are those that turn gene expression on or off in response to chemical or physical stimuli, including the presence of a regulatory compound. Regulatory sequences in prokaryotic systems include the lac, tac, and trp operator systems. In yeast, the ADH2 or GAL1 system may be used. In filamentous fungi, the Aspergillus niger glucoamylase promoter, Aspergillus oryzae TAKA α-amylase promoter, Aspergillus oryzae glucoamylase promoter, Trichoderma reesei cellobiohydrolase I promoter, and Trichoderma reesei cellobiohydrolase II promoter may be used. Other examples of regulatory sequences are those that allow for gene amplification. In eukaryotic systems, these regulatory sequences include the dihydrofolate reductase gene, which is amplified in the presence of methotrexate, and the metallothionein gene, which is amplified with heavy metals. In these cases, the polynucleotide encoding the polypeptide would be operably linked to the regulatory sequence.

[0545] Expression vector The present invention also relates to recombinant expression vectors comprising the polynucleotides of the present invention, promoters, and transcriptional and translational stop signals. The various nucleotides and control sequences combine to generate a recombinant expression vector, which may contain one or more convenient restriction sites to allow for insertion or substitution of a polynucleotide encoding a polypeptide at such sites. Alternatively, a polynucleotide can be expressed by inserting the polynucleotide or a nucleic acid construct comprising the polynucleotide into an appropriate vector for expression. In forming an expression vector, a coding sequence is placed in the vector such that the coding sequence is operably linked to appropriate control sequences for expression. The recombinant expression vector may be any vector (e.g., a plasmid or virus) that can be conveniently subjected to recombinant DNA techniques and can result in expression of a polynucleotide. The choice of vector will typically depend on the compatibility of the vector with the host cell into which the vector will be introduced. The vector may be a linear or closed circular plasmid.

[0546] The vector may be a self-replicating vector (i.e., a vector that exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication), such as a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain some means for ensuring self-replication. Alternatively, the vector may be one that, upon introduction into a host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid, or two or more vectors or plasmids that together contain the total DNA to be introduced into the genome of the host cell, or a transposon, may be used.

[0547] Vectors preferably contain one or more selectable markers which permit easy selection of cells that have been transformed, transfected, transduced, etc. A selectable marker is a gene the product of which confers biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, etc.

[0548] Examples of bacterial selectable markers are the Bacillus licheniformis or Bacillus subtilis dal genes, or markers that confer antibiotic resistance, such as ampicillin, chloramphenicol, kanamycin, neomycin, spectinomycin, or tetracycline resistance. Suitable markers for yeast host cells include, but are not limited to, ADE2, HIS3, LEU2, LYS2, MET3, TRP1, and URA3. Selectable markers for use in filamentous fungal host cells include, but are not limited to, adeA (phosphoribosylaminoimidazole-succinocarboxamide synthase), adeB (phosphoribosylaminoimidazole synthase), amdS (acetamidase), argB (ornithine carbamoyltransferase), bar (phosphinothricin acetyltransferase), hph (hygromycin phosphotransferase), niaD (nitrate reductase), pyrG (orotidine-5'-phosphate decarboxylase), sC (sulfate adenyltransferase), and trpC (anthranilate synthase), and equivalents thereof. The Aspergillus nidulans or Aspergillus oryzae amdS and pyrG genes and the Streptomyces hygroscopicus bar gene are preferred for use in Aspergillus cells, and the adeA, adeB, amdS, hph, and pyrG genes are preferred for use in Trichoderma cells.

[0549] The selection marker may be the dual selection marker system described in WO 2010 / 039889. In one aspect, the dual selection marker is the hph-tk dual selection marker system.

[0550] Preferably, the vector contains elements that allow for integration of the vector into the genome of the host cell or for autonomous replication of the vector in the cell independent of the genome.

[0551] For integration into the host cell genome, the vector may rely on the sequence of a polynucleotide encoding a polypeptide or any other element of the vector for integration into the genome by homologous or non-homologous recombination. Alternatively, the vector may contain additional polynucleotides that result in integration into the host cell genome at a precise location in the chromosome by homologous recombination. To increase the likelihood of integration at a precise location, the integrative elements should contain a sufficient number of nucleic acids, such as 100-10,000 base pairs, 400-10,000 base pairs, and 800-10,000 base pairs, with a high degree of sequence identity to the corresponding target sequence to increase the probability of homologous recombination. The integrative ele...

Claims

1. 1. A composition comprising at least 0.002 ppm of a polypeptide having DNase activity, wherein the composition is a detergent composition such as a laundry composition, wherein the polypeptide comprises the motif HXXP, where H is histidine, P is proline and X is any amino acid, wherein the polypeptide belongs to the GYS clade and comprises one or both of the motifs [M / L][S / T]GYSR[D / N] (SEQ ID NO:204) or ASXNRSKG (SEQ ID NO:205), wherein the polypeptide having DNase activity comprises or consists of the amino acid sequence of SEQ ID NO:21, or a polypeptide having at least 85% sequence identity thereto, and wherein the composition further comprises at least one surfactant, and is formulated as a bar, a homogenous tablet, and a tablet having two or more layers, a pouch with one or more compartments, a powder, granules, paste, gel or liquid.

2. The composition of claim 1 , wherein the polypeptide having DNase activity comprises or consists of an amino acid sequence having at least 86% sequence identity to SEQ ID NO:

21.

3. The composition of claim 1 , wherein the polypeptide having DNase activity comprises or consists of an amino acid sequence having at least 87% sequence identity to SEQ ID NO:

21.

4. The composition of claim 1 , wherein the polypeptide having DNase activity comprises or consists of an amino acid sequence having at least 88% sequence identity to SEQ ID NO:

21.

5. The composition of claim 1 , wherein the polypeptide having DNase activity comprises or consists of an amino acid sequence having at least 89% sequence identity to SEQ ID NO:

21.

6. The composition of claim 1 , wherein the polypeptide having DNase activity comprises or consists of an amino acid sequence having at least 90% sequence identity to SEQ ID NO:

21.

7. The composition of claim 1, wherein the polypeptide having DNase activity comprises one or more motifs selected from the following motifs: [T / D / S][G / N]PQL (SEQ ID NO: 198), [G / T]Y[D / S][R / K / L] (SEQ ID NO: 199), [E / D / H]H / I / V / L / F / M]X[P / A / S] (SEQ ID NO: 200), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 201) and C[D / N]T[A / R] (SEQ ID NO: 202).

8. 2. The composition of claim 1, wherein the composition comprises one or more polyols selected from glycerol, (mono, di, or tri)propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol, and adonitol.

9. The composition comprises: i. at least one additional enzyme, and / or ii. at least one polymer The composition according to any one of claims 1 to 8, comprising:

10. 10. The composition of claim 9, wherein the composition comprises at least one additional enzyme selected from a protease, an amylase, or a lipase, and / or at least one surfactant selected from anionic and nonionic surfactants.

11. The composition of claim 10 , wherein the composition comprises a protease.

12. Use of a composition according to any one of claims 1 to 11 to reduce or remove biofilm from an article such as a fabric.

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