Novel heterocyclic-substituted pyrimidine derivatives exhibiting cancer cell growth inhibitory effects and pharmaceutical compositions containing the same

Abstract

The present invention provides a novel compound represented by Chemical Formula 1 or a salt thereof, and a pharmaceutical composition for treating lung cancer containing the same. The pyrimidine derivative compound represented by Chemical Formula 1 effectively inhibits the growth of ALK-mutated and EGFR-mutated cancer cells, and can therefore be useful in treating lung cancer.

Description

[Technical field]

[0001] This application claims the benefit of priority based on Korean Patent Application No. 10-2019-0137489 filed on October 31, 2019, and all contents disclosed in the documents of that Korean patent application are incorporated herein by reference.

[0002] The present invention relates to novel heterocycle-substituted pyrimidine derivatives which effectively inhibit the growth of cancer cells, and pharmaceutical compositions containing the same. [Background technology]

[0003] Non-small cell lung cancer (NSCLC) is a cancer-related disease with a very high prevalence and mortality rate worldwide. NSCLC is mainly caused by mutations and overexpression of tyrosine kinase enzymes, and anticancer drugs to treat it have been developed by targeting the inhibition of this enzyme's activity. NSCLC, which mainly occurs in East Asia, including Korea, often has epidermal growth factor receptor (EGFR) gene mutations, and drugs with relatively low toxicity and good therapeutic effects have been developed.

[0004] In addition, non-small cell lung cancer is caused by the expression and rearrangement of various tumor genes, such as ALK (anaplastic lymphoma kinase), KRAS, and ROS1 (Lancet Oncol 2011;12(2):175-80.).

[0005] ALK gene abnormalities (EML4-ALK transfusion, etc.) are observed in some patients with non-small cell lung cancer, and various tyrosine kinase inhibitors (TKIs) are being used clinically to treat such cancers. ALK-positive non-small cell lung cancer occurs due to the fusion of the ALK and EML4 genes (ALK-EML4 fusion), and the fusion of the two genes causes the normally dormant ALK gene to accelerate cell growth, and cells that receive this signal quickly metastasize to cancer cells. Crizotinib, a representative treatment drug for patients with the above mutations, was approved by the US FDA in 2011 as a multi-targeted anti-cancer drug. This drug is used to treat metastatic and ALK-positive non-small cell lung cancer by suppressing the activity of MET, ALK, ROS1, etc. According to the results of clinical studies on crizotinib, the participants were mainly lung cancer patients with adenocarcinoma tissue type, and 46% were Asian. The treatment showed excellent efficacy, with a tumor response rate of approximately 65% ​​and a progression-free survival time of 7.7 months (3 months in the anti-cancer chemotherapy group). The most commonly reported abnormal reactions were visual field abnormalities, diarrhea, vomiting, edema, and nausea (J Thorac Oncol 2012;7(7):1086-90.).

[0006] Resistance to crizotinib inevitably occurs, and it has been reported that this is mainly due to secondary mutations in the ALK kinase domain (approximately 30%), amplification of ALK fusion mutation genes, and activation of bypass signaling processes. There are many different mutations, including secondary mutations including L1196M and G1269A, and the most frequent is L1196M, which is located at the gatekeeper residue and induces interference with the binding of crizotinib to ALK (J Clin Oncol 2013;31(8):1105-11).

[0007] Activating mutations (del119, L858R) in the kinase domain of the epidermal growth factor receptor (EGFR) have been found in oncogenes in some patients with non-small cell lung cancer, and small molecule EGFR kinase inhibitors such as gefitinib and erlotinib are used to treat this condition (Science 2004, 304:1497-500; and New England Journal of Medicine 2004, 350:2129-39).

[0008] When gefitinib or erlotinib is used as a treatment for non-small cell lung cancer patients with confirmed EGFR activating mutations, most patients develop resistance to the drugs within one year (Clinical Cancer Research 2013;19:2240-7). Among these resistance mechanisms, the T790M mutation of the epidermal growth factor receptor is observed in up to 60% of cases. Therefore, a third generation EGFR inhibitor that targets the T790M mutant epidermal growth factor receptor (EGFR) in lung cancer has been developed. Representative drugs include osimertinib and lasertinib, which target the T790M mutation and show relatively low toxicity, and are used clinically to treat this type of non-small cell lung cancer (J Thorac Dis. 2018 Jul;10(7):3909-3921).

[0009] However, drug resistance to the above-mentioned third-generation EGFR inhibitors has inevitably been reported, with C797S mutation and MET amplification reported as the main resistance mechanisms (J Hematol Oncol. 2016, Jul 22; 9(1): 59; and Nature Medicine 2015, 21, 560-562; and Lung Cancer 2018, 118, 105-110; and ASCO 2017 abstract 2572, 9020). It has been reported that C797S mutation and MET amplification can be found separately or simultaneously.

[0010] In non-small cell lung cancer caused by ALK mutation or EGFR mutation (or both), the main resistance mechanism is secondary mutation that inhibits the binding strength of kinases and drugs, and it has been reported that such mutations affect downstream signaling in cells (Eur Med Chem. 2017 Aug 18; 136: 497-510.). Although the development of various ALK and EGFR inhibitors is progressing continuously, the development of inhibitors that inhibit both kinases simultaneously is progressing very slowly. Therefore, there is a need to develop drugs that effectively suppress the growth of ALK mutation or EGFR mutation cancer cells, which are the main drug resistance mechanisms mentioned above. [Prior art documents] [Patent documents]

[0011] [Patent Document 1] PCT Patent Publication WO / 2009 / 143389A1 Summary of the Invention [Problem to be solved by the invention]

[0012] The present inventors have been working to develop novel compounds that effectively inhibit ALK-mutated cancer and EGFR-mutated cancer. As a result, they have found novel heterocycle-substituted pyrimidine derivatives that are effective in cancer treatment. In particular, they have confirmed that the novel heterocycle-substituted pyrimidine derivatives exhibit excellent effects in the treatment of lung cancer.

[0013] Therefore, an object of the present invention is to provide novel heterocycle-substituted pyrimidine derivatives which are effective in treating cancer.

[0014] Another object of the present invention is to provide a pharmaceutical composition for treating lung cancer, which comprises the heterocycle-substituted pyrimidine derivative.

[0015] Another object of the present invention is to provide a pharmaceutical composition for treating lung cancer, particularly lung cancer expressing ALK mutation or EGFR mutation.

[0016] Another object of the present invention is to provide a pharmaceutical composition for treating lung cancer, particularly lung cancer expressing ALK mutation and EGFR mutation. [Means for solving the problem]

[0017] In order to achieve the above object, the present invention provides A compound represented by the following chemical formula 1 or a salt thereof:

[0018] [ka]

[0019] X is oxygen, an amine group unsubstituted or substituted with a C1 to C4 alkyl group, or a C1 to C4 alkyl group; R1 is a C1 to C4 alkyl group, a C3 to C6 cycloalkyl group, CF3, or a dimethylamine group; R2 is H or a C1 to C4 alkyl group; R3 is hydrogen or a halogen group. R4 is hydrogen, a halogen group, CN, CF3, a C1 to C4 alkyl group, or an aminocarbonyl group; R5 is hydrogen or a C1 to C4 alkyl group; R6 is hydrogen or a C1 to C4 alkyl group; R7 is a heterocyclic compound consisting of one or more nitrogen atoms and 2 to 10 carbon atoms, substituted or unsubstituted with C1 to C4 alkyl groups; R8 is an aliphatic heterocyclic compound consisting of 2 to 10 carbon atoms and one or more heteroatoms selected from nitrogen, oxygen and sulfur atoms, substituted or unsubstituted with C1 to C4 alkyl groups; or N 1 ,N 1 ,N 2 - a tri(C1-C4 alkyl)ethylenediamine group, In the above, when X is oxygen, X forms a double bond with S and S forms a single bond with N, and when X is an amine group substituted or unsubstituted with a C1 to C4 alkyl group or a C1 to C4 alkyl group, X forms a single bond with S and S forms a double bond with N.

[0020] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: The present invention provides a compound represented by the above formula 1 or a salt thereof for use in treating lung cancer.

[0021] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: The present invention provides a pharmaceutical composition for treating lung cancer, which comprises a compound represented by the above formula 1 or a pharma- ceutically acceptable salt thereof as an active ingredient and a pharma- ceutically acceptable carrier.

[0022] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: A method for treating an animal having lung cancer is provided, which comprises administering to the animal an effective amount of a compound represented by Formula 1 above.

[0023] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: The present invention provides a use of the compound represented by Chemical Formula 1 for treating lung cancer. Effect of the Invention

[0024] The novel heterocyclic ring-substituted pyrimidine derivative compounds of the present invention provide excellent effects in the treatment of cancer.

[0025] In addition, the pharmaceutical composition for treating lung cancer of the present invention containing the above heterocycle-substituted pyrimidine derivative compound exhibits excellent activity in treating lung cancer, and in particular, effectively inhibits the growth of ALK-mutated cancer cells and EGFR-mutated cancer cells. BEST MODE FOR CARRYING OUT THEINVENTION

[0026] Hereinafter, the present invention will be described in detail with reference to specific examples. However, these are presented as examples, and the present invention is not limited thereto, and the present invention is only defined by the scope of the claims below. Furthermore, detailed descriptions of configurations that are necessary to implement the present invention but that can be easily implemented by ordinary skilled artisans from known techniques will be omitted.

[0027] Hereinafter, unless otherwise specified, the term "compound of the present invention" or "compound of Chemical Formula 1" is used to include both the compound itself and a salt thereof.

[0028] As used herein, the term "alkyl group" refers to straight and branched chain hydrocarbon groups having the specified number of carbon atoms. The alkyl group may be, for example, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, i-butyl, t-butyl, and the like.

[0029] As used herein, the term "alkylsulfonyl" refers to alkyl-S(O2)-, where alkyl is defined above.

[0030] The present invention relates to a compound represented by the following chemical formula 1 or a salt thereof: A compound represented by the following chemical formula 1 or a salt thereof:

[0031] [ka]

[0032] X is oxygen, an amine group unsubstituted or substituted with a C1 to C4 alkyl group, or a C1 to C4 alkyl group; R1 is a C1 to C4 alkyl group, a C3 to C6 cycloalkyl group, CF3, or a dimethylamine group; R2 is H or a C1 to C4 alkyl group; R3 is hydrogen or a halogen group. R4 is hydrogen, a halogen group, CN, CF3, a C1 to C4 alkyl group, or an aminocarbonyl group; R5 is hydrogen or a C1 to C4 alkyl group; R6 is hydrogen or a C1 to C4 alkyl group; R7 is a heterocyclic compound consisting of one or more nitrogen atoms and 2 to 10 carbon atoms, substituted or unsubstituted with C1 to C4 alkyl groups; R8 is an aliphatic heterocyclic compound consisting of 2 to 10 carbon atoms and one or more heteroatoms selected from nitrogen, oxygen and sulfur atoms, substituted or unsubstituted with C1 to C4 alkyl groups; or N 1 ,N 1 ,N 2 - a tri(C1-C4 alkyl)ethylenediamine group, In the above, when X is oxygen, X forms a double bond with S and S forms a single bond with N, and when X is an amine group substituted or unsubstituted with a C1 to C4 alkyl group or a C1 to C4 alkyl group, X forms a single bond with S and S forms a double bond with N.

[0033] In addition, for R7 and R8, R7 is an aromatic heterocyclic compound having one or more nitrogen atoms and 2 to 10 carbon atoms, which may or may not be substituted with a C1 to C4 alkyl group; R8 is an aliphatic heterocyclic compound having 2 to 10 carbon atoms and two or more heteroatoms selected from nitrogen atoms, oxygen atoms, and sulfur atoms, which are substituted or unsubstituted with a C1 to C4 alkyl group; or N 1 ,N 1 ,N 2-tri(C1 to C4 alkyl)ethylenediamine group.

[0034] In addition, for R7 and R8, R7 is a pyrazolyl group substituted or unsubstituted with a C1 to C4 alkyl group, an imidazolyl group substituted or unsubstituted with a C1 to C4 alkyl group, or a triazolyl group substituted or unsubstituted with a C1 to C4 alkyl group; R8 is a morpholinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a thiomorpholinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a piperazinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a C5 to C10 diazospiro compound substituted or unsubstituted with a C1 to C4 alkyl group, a C5 to C10 oxoazaspiro compound substituted or unsubstituted with a C1 to C4 alkyl group, a C5 to C10 thioazaspiro compound substituted or unsubstituted with a C1 to C4 alkyl group, or N 1 ,N 1 ,N 2 -tri(C1 to C4 alkyl)ethylenediamine group.

[0035] The R7 is selected from the following compounds:

[0036] [ka]

[0037] The R8 may be selected from the following compounds:

[0038] [ka]

[0039] In the compound represented by Chemical Formula 1 of the present invention or a salt thereof, X is oxygen, R1 is a C1 to C4 alkyl group, R2 is H or a C1 to C4 alkyl group; R3 is hydrogen or a halogen group. R4 is hydrogen or a halogen group. R5 is hydrogen or a C1 to C4 alkyl group; R6 is a C1 to C4 alkyl group; R7 is selected from the following compounds:

[0040] [ka]

[0041] R8 can be selected from the following compounds:

[0042] [ka]

[0043] Specifically, the compound represented by the above formula 1 may be selected from the following compounds: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 1), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 2), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methoxymethanesulfonamide (chemical formula 3), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methoxymethanesulfonamide (chemical formula 4), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 5), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 6), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 7), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 8), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 9), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 10).

[0044] In the present invention, the salt may be in the form of a salt derived from one or more acids selected from the group consisting of hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, acetic acid, glycolic acid, lactic acid, pyruvic acid, malonic acid, succinic acid, glutaric acid, fumaric acid, malic acid, mandelic acid, tartaric acid, citric acid, ascorbic acid, palmitic acid, maleic acid, benzoic acid, hydroxybenzoic acid, phenylacetic acid, cinnamic acid, salicylic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, and toluenesulfonic acid.

[0045] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: The present invention relates to a pharmaceutical composition for treating lung cancer, which comprises as an active ingredient a compound represented by the above formula 1 or a pharma- ceutically acceptable salt thereof and a pharma- ceutically acceptable carrier.

[0046] The lung cancer may be an ALK-mutated or Epidermal Growth Factor Receptor (EGFR)-mutated lung cancer.

[0047] The lung cancer may be non-small cell lung cancer.

[0048] The pharmaceutical composition of the present invention can be particularly useful for treating lung cancer, and among lung cancers, can be effectively used for treating lung cancer having ALK mutation or EGFR mutation cancer cells.

[0049] The pharmaceutical composition of the present invention has the effect of simultaneously inhibiting ALK (L1196M or EML4-ALK amplification mutation) and EGFR (C797S).

[0050] In the present invention, the compound represented by Chemical Formula 1 may be used in the form of a salt derived from an inorganic acid or an organic acid, for example, one or more acids selected from the group consisting of hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, acetic acid, glycolic acid, lactic acid, pyruvic acid, malonic acid, succinic acid, glutaric acid, fumaric acid, malic acid, mandelic acid, tartaric acid, citric acid, ascorbic acid, palmitic acid, maleic acid, benzoic acid, hydroxybenzoic acid, phenylacetic acid, cinnamic acid, salicylic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, and toluenesulfonic acid.

[0051] The present invention also provides a method for producing a method for manufacturing a semiconductor device comprising the steps of: The present invention relates to a compound represented by the above formula 1 or a salt thereof for use in treating lung cancer.

[0052] The present invention also relates to a method for treating an animal having lung cancer, which comprises administering to the animal an effective amount of a compound represented by Chemical Formula 1 above.

[0053] The present invention also relates to the use of the compound represented by Chemical Formula 1 in the treatment of lung cancer, particularly non-small cell lung cancer.

[0054] The animal may be a human and the lung cancer may be a lung cancer having an ALK mutation or an EGFR mutation in cancer cells.

[0055] The ALK mutation may be L1196M or EML4-ALK fusion mutation, and the EGFR mutation may be one or more mutations selected from L858R, T790M, del19, and C797S.

[0056] The pharmaceutical compositions of the present invention can be formulated by a conventional method and prepared in various oral dosage forms such as tablets, pills, powders, capsules, syrups, emulsions, microemulsions, etc., or parenteral dosage forms such as intravenous injection, subcutaneous injection, intramuscular injection, peritoneal injection, transdermal injection, and direct injection into tissues.

[0057] When the pharmaceutical composition of the present invention is prepared in the form of an oral dosage form, a pharma- ceutically acceptable carrier may be used without limitation from ingredients known in the art, so long as it does not interfere with the activity of the active ingredient.

[0058] Examples of the carrier include, but are not limited to, excipients, diluents, disintegrants, binders, lubricants, surfactants, emulsifiers, suspending agents, diluents, and the like.

[0059] When the pharmaceutical composition of the present invention is prepared in the form of an injection, any pharma- ceutically acceptable carrier may be used without limitation, as long as it does not interfere with the activity of the active ingredient, and is known in the art.

[0060] Specific examples include, but are not limited to, water, saline, glucose aqueous solution, analogous sugar aqueous solution, alcohol, glycol, ether (e.g., polyethylene glycol 400), oil, fatty acid, fatty acid ester, glyceride, surfactant, suspending agent, emulsifier, etc.

[0061] The dosage of the pharmaceutical composition of the present invention should be determined taking into consideration the age, sex, condition, absorption rate of the active ingredient in the body, inactivation rate, and drugs used in combination, and can be administered at 0.0001 mg / kg (body weight) to 100 mg / kg (body weight) per administration based on the compound of formula 1. The appropriate number of administrations is about 1 to 3 times a day. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0062] The present invention will be described in more detail with reference to the following examples. It will be obvious to those skilled in the art that these examples are provided to more specifically explain the present invention, and that the scope of the present invention is not limited to these examples according to the gist of the present invention.

[0063] <Method for synthesizing the compound represented by chemical formula 1> The compound represented by the following Chemical Formula 1 according to the present invention can be easily prepared, for example, by referring to the method represented by the following Reaction Scheme 1:

[0064] [ka]

[0065] Synthesis Example 1: Synthesis of N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide

[0066] Step A-1: ​​Synthesis of N-methyl-N-(2-nitrophenyl)methanesulfonamide Dissolve 1-fluoro-2-nitrobenzene (1.0 equivalent) in acetonitrile and add potassium carbonate (2.0 equivalent) and N-methylmethanesulfonamide (1.4 equivalent) at room temperature. Stir at 80°C overnight. After the reaction is complete, cool to room temperature and filter. Evaporate the filtrate under reduced pressure to obtain the compound. Use it in the next reaction without separation.

[0067] Step A-2: Synthesis of N-(2-aminophenyl)-N-methylmethanesulfonamide Dissolve N-methyl-N-(2-nitrophenyl)methanesulfonamide (1.0 eq.) in methanol and ethyl acetate (1:1) and add 10% palladium on charcoal (0.2 eq.). Stir under hydrogen for 2 hours. After completion of the reaction, filter through celite. Evaporate the filtrate under reduced pressure. Solidify with ethyl ether and pentane. Filter to obtain the target compound. Use it in the next reaction without separation.

[0068] Step A-3: Synthesis of N-(2-((2,5-dichloropyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide Dissolve N-(2-aminophenyl)-N-methylmethanesulfonamide (1.0 equivalent) in isopropyl alcohol and add 2,4,5-trichloropyrimidine (1.1 equivalent) and N,N-diisopropylethylamine (2.5 equivalent) at room temperature. Stir at 80°C overnight. After the reaction is complete, evaporate under reduced pressure and extract with water and dichloromethane. Wash the organic layer with 2N hydrochloric acid. Evaporate the organic layer under reduced pressure to obtain the target compound. Use it in the next reaction without separation.

[0069] Step B-1: Synthesis of 4-bromo-5-fluoro-2-nitrophenol Dissolve 4-bromo-3-fluorophenol in dichloromethane. Add concentrated sulfuric acid and nitric acid at 0°C. Stir at the same temperature for 2 hours. After the reaction is complete, neutralize with sodium bicarbonate saturated with water. Then extract with dichloromethane. Collect the organic layer and evaporate under reduced pressure to obtain the target compound. Use it for the next reaction without any further separation process.

[0070] Step B-2: Synthesis of 1-bromo-2-fluoro-4-methoxy-5-nitrobenzene Dissolve 4-bromo-5-fluoro-2-nitrophenol (1.0 equivalent) in N,N-dimethylformamide and add potassium carbonate (2.0 equivalent) and methyl iodide (1.5 equivalent) at room temperature. Stir at 45°C for 2 hours. After the reaction is complete, extract with water and ethyl acetate and collect the organic layer. Evaporate the organic layer under reduced pressure and use column chromatography to obtain the target compound (hexane:ethyl acetate=10:1).

[0071] Step B-3: Synthesis of 4-(2-fluoro-4-methoxy-5-nitrophenyl)-1-methyl-1H-pyrazole 1-Bromo-2-fluoro-4-methoxy-5-nitrobenzene (1.0 eq.) is dissolved in 1,4-dioxane and water, and 1-methylpyrazole-4-boronic acid pinacol ester (1.2 eq.), sodium carbonate (2.0 eq.), and palladium catalyst (0.1 eq.) are added at room temperature. The mixture is refluxed and stirred overnight. After the reaction is complete, the organic layer is extracted with water and ethyl acetate. The organic layer is evaporated under reduced pressure and the target compound is obtained by column chromatography (hexane:ethyl acetate=5:1 to 3:1).

[0072] Step B-4: Synthesis of 4-(5-methoxy-2-(1-methyl-1H-pyrazol-4-yl)-4-nitrophenyl)morpholine 4-(2-Fluoro-4-methoxy-5-nitrophenyl)-1-methyl-1H-pyrazole (1.0 eq.) is dissolved in N,N-dimethylformamide, and potassium carbonate (1.2 eq.) and morpholine (1.2 eq.) are added at room temperature. The mixture is stirred at 130°C overnight. After the reaction is complete, the organic layer is extracted with water and ethyl acetate. The organic layer is evaporated under reduced pressure and the target compound is obtained by column chromatography (hexane:ethyl acetate = 1:1 to 1:2).

[0073] Step B-5: Synthesis of 2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinoaniline Dissolve 4-(5-methoxy-2-(1-methyl-1H-pyrazol-4-yl)-4-nitrophenyl)morpholine (1.0 equivalent) in methyl alcohol and ethyl acetate (1:1) and add 10% palladium on charcoal (0.2 equivalent). Stir under hydrogen for 2 hours. After completion of the reaction, filter through celite. Evaporate the filtrate under reduced pressure. Solidify with hexane to obtain a solid, which is used in the next reaction without separation.

[0074] Step C-1: Synthesis of the final compound Dissolve pyrimidine derivative (1.0 equivalent) in isopropyl alcohol, add aniline derivative (1.0 equivalent) and methanesulfonyl acid (1.3 equivalent) at room temperature. Stir at 80°C overnight. After the reaction is complete, remove the solvent by evaporating under reduced pressure, and extract with water and a 10% methanol / dichloromethane mixture. Evaporate the organic layer under reduced pressure and use column chromatography to obtain the target compound (10% methyl alcohol / dichloromethane).

[0075] Example 1: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (Compound 1)

[0076] [ka]

[0077] The final compound was prepared as described above.

[0078] Yield: 69.5%, White solid, 1 H NMR(400MHz,DMSO-d6)δ 8.25(d,J=2.4 Hz,2H), 8.16(d,J=8.6 Hz,1H), 8.10(s,1H), 8.00(s,1H), 7.79(d,J=0.8 Hz,1H), 7.56(s,1H), 7.52(dd,J=8.0, 1.5 Hz,1H), 7.02(t,J=7.6 Hz,1H), 6.83(bs,1H), 6.76(s,1H), 3.80(s,3H), 3.76(s,3H), 3.70(m,4H), 3.14(s,3H), 3.05(s,3H), 2.80(m,4H). MS: ESI m / z 599.04[M+H]+

[0079] Example 2: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (compound 2))

[0080] [ka]

[0081] It was synthesized using methanesulfonamide in Step A-1.

[0082] Yield: 62.6%, White solid, 1 H NMR(400MHz,DMSO-d6)δ 9.26(s,1H), 8.42(s,1H), 8.08(d,J=1.8 Hz,2H), 7.98 -7.87(m,2H), 7.77(d,J=0.8 Hz,1H), 7.56(s,1H), 7.26(dd,J=8.0, 1.5 Hz,1H), 6.99(t,J=7.6 Hz,1H), 6.80(s,1H), 6.73(s,1H), 3.80(s,3H), 3.75(s,3H), 3.68(m,4H), 2.92(s,3H), 2.83 -2.72(m,4H). MS: ESI m / z 585.05[M+H]+

[0083] Example 3: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methoxymethanesulfonamide (compound 3)

[0084] [ka]

[0085] It was synthesized using thiomorpholine in Step B-4.

[0086] Yield: 58.9%, White solid, 1 H NMR(400MHz,DMSO-d6)δ 8.26(d,J=2.4 Hz,2H), 8.14(d,J=8.6 Hz,1H), 8.09(s,1H), 8.01(s,1H), 7.79(d,J=0.8 Hz,1H), 7.56(s,1H), 7.52(dd,J=8.0, 1.5 Hz,1H), 7.02(t,J=7.6 Hz,1H), 6.83(bs,1H), 6.76(s,1H), 3.94(s,3H), 3.86(s,3H), 3.72(m,4H), 3.33(d,J=12.0Hz, 5H), 2.95(m,4H). MS: ESI m / z 615.04[M+H]+

[0087] Example 4: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methoxymethanesulfonamide (compound 4)

[0088] [ka]

[0089] It was synthesized using N-methylpiperazine in Step B-4.

[0090] Yield:61.5%, Off-white solid, 1 H NMR(400MHz,DMSO-d6)δ 8.24(d,J=2.4 Hz,2H), 8.13(d,J=8.6 Hz,1H), 8.11(s,1H), 7.98(s,1H), 7.77(d,J=0.8 Hz,1H), 7.53(s,1H), 7.51(dd,J=8.0, 1.5 Hz,1H), 7.02(t,J=7.6 Hz,1H), 6.83(bs,1H), 6.76(s,1H), 3.94(s,3H), 3.87(s,3H), 3.42‐3.25(m,11H), 3.19 -3.07(m,2H), 2.96(m,2H), 2.26(s,3H). MS:ESI m / z 612.02[M+H]+

[0091] Example 5: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (compound 5)

[0092] [ka]

[0093] The synthesis was carried out using 2-oxa-6-azaspiro[3.3]heptane in Step B-4.

[0094] Yield: 45.3%, White solid, 1 H NMR(400MHz,DMSO‐d6)δ 8.28(d,J=2.4 Hz,2H), 8.14(d,J=8.6 Hz,1H), 8.12(s,1H), 8.02(s,1H), 7.81(bs,1H), 7.57(s,1H), 7.54(m,1H), 7.02(t,J=7.6 Hz,1H), 6.83(bs,1H), 6.76(s,1H), 4.61(s,4H), 3.94(s,3H), 3.87(s,3H), 3.59(s,4H), 3.32(s,3H), 3.31(s,3H). MS:ESI m / z 611.09[M+H]+

[0095] Example 6: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (compound 6)

[0096] [ka]

[0097] The synthesis was carried out using 2-methyl-2,6-diazaspiro[3.3]heptane in Step B-4.

[0098] Yield:40.1%, Off-white solid, 1 H NMR(400MHz,DMSO-d6)δ 8.28(d,J=2.4 Hz,2H), 8.17(d,J=8.6 Hz,1H), 8.13(s,1H), 8.02(s,1H), 7.81(d,J=0.8 Hz,1H), 7.55(s,1H), 7.52(dd,J=8.0, 1.5 Hz,1H), 7.02(t,J=7.6 Hz,1H), 6.83(bs,1H), 6.76(s,1H), 3.94(s,3H), 3.87(s,3H), 3.59(s,4H), 3.35(s,3H), 3.33(s,3H), 3.22(s,4H), 2.12(s,3H). MS:ESI m / z 624.08[M+H]+

[0099] Example 7: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (compound 7)

[0100] [ka]

[0101] In Step B-4, N-methanesulfonamide was synthesized using thiomorpholine.

[0102] Yield:53.6%, Off-white solid, 1H NMR(400MHz,DMSO-d6)δ 9.25(s,1H), 8.40(s,1H), 8.06(d,J=1.8 Hz,2H), 7.96 -7.85(m,2H), 7.73(d,J=0.8 Hz,1H), 7.55(s,1H), 7.22(dd,J=8.0, 1.5 Hz,1H), 6.96(t,J=7.6 Hz,1H), 6.78(s,1H), 6.71(s,1H), 3.94(s,3H), 3.87(s,3H), 3.72(m,4H), 3.10(m,4H), 2.90(s,3H). MS:ESI m / z 601.02[M+H]+

[0103] Example 8: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (compound 8)

[0104] [ka]

[0105] In Step B-4, N-methanesulfonamide was synthesized using N-methylpiperazine.

[0106] Yield: 63.4%, White solid,

[0107] 1 H NMR(400MHz,DMSO-d6)δ 9.29(s,1H), 8.49(s,1H), 8.02-7.87(m,4H), 7.77(d,J=0.8 Hz,1H), 7.56(s,1H), 7.26(dd,J=8.0, 1.5 Hz,1H), 7.00(t,J=7.6 Hz,1H), 6.83(s,1H), 6.73(s,1H), 3.94(s,3H), 3.87(s,3H), 3.42‐3.25(m,5H), 3.19 -3.07(m,2H), 2.96(m,2H), 2.90(s,3H), 2.16(s,3H). MS: ESI m / z 598.01[M+H]+

[0108] Example 9: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (compound 9)

[0109] [ka]

[0110] In Step B-4, N-methanesulfonamide was synthesized using 2-oxa-6-azaspiro[3.3]heptane.

[0111] Yield: 40.1%, White solid, 1 H NMR(400MHz,DMSO-d6)δ 9.25(s,1H), 8.40(s,1H), 8.07(d,J=1.8 Hz,2H), 7.97 -7.89(m,2H), 7.75(d,J=0.8 Hz,1H), 7.54(s,1H), 7.23(dd,J=8.0, 1.5 Hz,1H), 6.98(t,J=7.6 Hz,1H), 6.81(s,1H), 6.71(s,1H), 4.61(s,4H), 3.94(s,3H), 3.87(s,3H), 3.59(s,4H), 2.90(s,3H). MS:ESI m / z 597.03[M+H]+

[0112] Example 10: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (compound 10)

[0113] [ka]

[0114] In Step B-4, N-methanesulfonamide was synthesized using 2-methyl-2,6-diazaspiro[3.3]heptane.

[0115] Yield:37.2%, Off-white solid, 1 H NMR(400MHz,DMSO-d6)δ 9.46(s,1H), 8.82(s,1H), 8.28(m,2H), 7.94 -7.82(m,2H), 7.70(d,J=0.8 Hz,1H), 7.53(s,1H), 7.22(dd,J=8.0, 1.5 Hz,1H), 6.94(t,J=7.6 Hz,1H), 6.81(s,1H), 6.70(s,1H), 3.94(s,3H), 3.87(s,3H), 3.59(s,4H), 3.22(s,4H), 2.92(s,3H), 2.15(s,3H). MS: ESI m / z 610.08[M+H]+

[0116] Experimental example 1: Kinase inhibitory activity measurement The kinase inhibitory activity of the compound in Example 1 was measured against ALK mutations and EGFR mutations, and the results are shown in Table 1 below. The kinase inhibitory activity was measured by the following method. The IC 50 The values ​​were calculated and the results are shown in Table 1 below as A, B, C and D, where A is the IC 50 ≦50nM, B is IC 50 50-100nM, C is IC 50 >100 nM. Crizotinib, Alectinib, and Osimertinib were used as control drugs.

[0117] 1. Each kinase was cultured in 8 mM MOPS, pH 7.0, 0.2 mM EDTA, 250 μM KKKGQEEEYVFIE, 1 mM sodium orthovanadate, 5 mM sodium‐6‐glycerophosphate, 10 mM magnesium acetate, and [η‐33P]‐ATP.

[0118] 2. The compound to be evaluated (DMSO solution) and Mg / ATP were added and reacted.

[0119] 3. After about 40 minutes at room temperature, 10 uL of 0.5% phosphoric acid was added to terminate the reaction.

[0120] 4. The reaction mixture was aliquoted at 0.5%, 10uL, and spotted onto a P30 filtermat.

[0121] 5. Wash four times with 0.425% phosphoric acid for about 4 minutes. Wash once with methanol, then dry and analyze by scintillation counting to determine IC 50 The values ​​were measured.

[0122] [Table 1]

[0123] As shown in the experimental results in Table 1 above, it was confirmed that the compounds prepared according to the embodiments of the present invention have much superior ALK and EGFR kinase inhibitory activity compared to crizotinib, alectinib, and osimertinib.

[0124] In other words, unlike conventional crizotinib, alectinib, and osimertinib that target ALK and EGFR separately, the compound of the present invention is not only a targeted therapeutic agent that can simultaneously target ALK and EGFR, but also inhibits the C797S EGFR mutant protein that is resistant to osimertinib, and has been confirmed to have excellent effects.

[0125] Experimental Example 2: Measurement of the effect of inhibiting cancer cell growth The growth inhibitory effect of the compounds obtained in the above examples on ALK mutant cancer cells and EGFR mutant Ba / F3 cancer cell lines was measured. The anticancer efficacy measurement using ALK mutant sell lines such as H3122 (EML4-ALK v1) and H2228 (EML4-ALK v3) and EGFR mutant Ba / F3 stable cell line was carried out as follows.

[0126] Gene construction: Wild type and mutant EGFR were purchased from Addgene (wild type, #11011; L858R, #11012; L858R+T790M, #32073; del19, #32062; del19+T790M, #32072). All constructions were retroviral vectors, and viral particles were finally prepared for infection.

[0127] Construction of Ba / F3 stable cell line: Murine lymphoid cells grow IL-3 dependently. When these cell lines are infected with each mutant EGFR construction, the expression of mutant EGFR leads to oncogenic addiction, and the cells can survive without IL-3. Using this principle, we constructed stable cell lines without puromycin selection. In brief, Ba / F3 was infected with each construction, and after 48 hours, IL-3 was removed while media exchange was performed, and the cells were cultured. However, in the case of wild type EGFR, puromycin selection was performed.

[0128] <Confirmation of Ba / F3 stable cell line> For all stable cell lines, western blotting was performed to confirm the expression of each construct and EGFR activity (EGFR wild type and L858R were not detected).

[0129] <Confirmation of changes in cellular kinase activity (Western blotting)> Each stable cell line was treated with drugs in a concentration-dependent manner, and cells were harvested 5 hours later. Cell lysates were prepared using EBC lysis buffer (50 mM Tris-HCl [pH 8.0], 120 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA, 0.3 mM phenylmethylsulfonylfluoride, 0.2 mM sodium orthovanadate, 0.5% NP-40, and 5 U / mL aprotinin). The activity was measured using antibodies against EGFR-related signaling molecules [p-EGFR (Tyr1173), EGFR, Akt, p-Erk, Erk, actin, from Santa Cruz; p-Akt, from Cell signaling].

[0130] <Verification of anti-cancer effect through MTT assay> 2×10 5 cells were seeded in 96-well plates. After 24 hours, each drug was treated in a dose-dependent manner, followed by 72-hour incubation. Then, 15 μL of MTT reagent was added and reacted for 4 hours, and then 100 μL of 10% SDS was added and incubated for 24 hours. The change in the final OD was read at 595 nm. MTT result analysis was performed through prism software to obtain IC 50The values ​​were measured.

[0131] The IC value was determined by the concentration at which each compound inhibited cell growth by 50%. 50 The values ​​were calculated, and the results are shown in Table 2 below. Crizotinib, Alectinib, and Osimertinib were used as control drugs. The results are shown in Table 2 below with A, B, C, and D. Here, A is the IC 50 ≦100nM, B is IC 50 100-500nM, C is IC 50 > 500nM.

[0132] [Table 2]

[0133] As shown in the experimental results in Table 2 above, it was confirmed that the compounds prepared according to the examples of the present invention exhibited significantly more inhibitory activity against the mutation-expressing cancer cell lines than crizotinib, alectinib, and osimertinib.

[0134] As shown in the in vitro kinase assay results of Experimental Example 1, the compounds of the present invention are not only targeted therapeutic agents capable of simultaneously targeting ALK and EGFR, but also have excellent effects in inducing the death of cancer cells expressing C797S EGFR mutant protein, which is resistant to osimertinib.

Claims

1. A compound represented by the following chemical formula 1 or a salt thereof: 【Chemistry 1】 X is oxygen, R1 is a C1 to C4 alkyl group; R2 is H or a C1 to C4 alkyl group; R3 is hydrogen or a halogen group; R4 is hydrogen or a halogen group; R5 is hydrogen; R6 is a C1 to C4 alkyl group; R7 is a pyrazolyl group substituted or unsubstituted with a C1 to C4 alkyl group, an imidazolyl group substituted or unsubstituted with a C1 to C4 alkyl group, or a triazolyl group substituted or unsubstituted with a C1 to C4 alkyl group; R8 is a morpholinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a thiomorpholinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a piperazinyl group substituted or unsubstituted with a C1 to C4 alkyl group, a C5 to C10 diazospiroheptanyl group substituted or unsubstituted with a C1 to C4 alkyl group, a C5 to C10 oxoazaspiroheptanyl group substituted or unsubstituted with a C1 to C4 alkyl group, or a C5 to C10 thioazaspiroheptanyl group substituted or unsubstituted with a C1 to C4 alkyl group; In the above, X forms a double bond with S and S forms a single bond with N.

2. R7 is selected from the following: 【Chemistry 2】 The compound or salt thereof represented by formula 1 according to claim 1, wherein R8 is selected from the following: 【Chemistry 3】

3. X is oxygen, R1 is a C1 to C4 alkyl group; R2 is H or a C1 to C4 alkyl group; R3 is hydrogen or a halogen group; R4 is hydrogen or a halogen group; R5 is hydrogen; R6 is a C1 to C4 alkyl group; R7 is selected from: 【Chemistry 4】 The compound or salt thereof represented by formula 1 according to claim 1, wherein R8 is selected from the following: 【Chemistry 5】

4. The compound represented by the formula 1 according to claim 1 or a salt thereof is selected from the following compounds: N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 1), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-morpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 2), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 3), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (chemical formula 4), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (formula 5), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)-N-methylmethanesulfonamide (formula 6), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-thiomorpholinophenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (formula 7), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(4-methylpiperazin-1-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (formula 8), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(2-oxa-6-azaspiro[3.3]heptan-6-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (chemical formula 9), N-(2-((5-chloro-2-((2-methoxy-5-(1-methyl-1H-pyrazol-4-yl)-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)pyrimidin-4-yl)amino)phenyl)methanesulfonamide (formula 10).

5. 2. The compound or salt thereof according to claim 1, wherein the salt is in the form of a salt derived from one or more acids selected from the group consisting of hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, acetic acid, glycolic acid, lactic acid, pyruvic acid, malonic acid, succinic acid, glutaric acid, fumaric acid, malic acid, mandelic acid, tartaric acid, citric acid, ascorbic acid, palmitic acid, maleic acid, benzoic acid, hydroxybenzoic acid, phenylacetic acid, cinnamic acid, salicylic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, and toluenesulfonic acid.

6. 10. A pharmaceutical composition for treating lung cancer, comprising as an active ingredient a compound according to any one of claims 1 to 5 or a pharma- ceutically acceptable salt thereof and a pharma- ceutically acceptable carrier.

7. 7. The pharmaceutical composition for treating lung cancer according to claim 6, wherein the lung cancer is ALK-mutated and EGFR (Epidermal Growth Factor Receptor)-expressing lung cancer.

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