Sample quality assessment method

Abstract

To provide a method of obtaining biological samples of improved quality.SOLUTION: The present invention relates to provision of methods of obtaining biological samples of improved quality, and encompasses identification of markers or proteins in biological samples that are altered due to variations in sample collection, handling and processing. The invention is also useful for correcting variations in measured results for disease biomarkers and allows samples or groups of samples to be rejected as necessary if it is determined that a collection method for the sample was not in accordance with a predetermined protocol.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] In medical diagnostics and drug development, comparisons are made between blood samples and other biological samples from individuals to measure and understand changes that may be related to certain diseases and disorders. For example, biomarkers may indicate whether a particular drug can be responded to, whether a disease such as cancer is present, or to monitor processes such as response to treatment or changes in organ function. If established to be reliable and stable, such biomarker measurements may be used in clinical practice.

[0002] Important properties for an ideal biomarker measurement, necessary to be discovered and reach clinical utility, include reliability and stability. [Background technology]

[0003] Blood contains powerful cellular and humoral systems to respond to injury or foreign and infectious agents. Small scale exposures can trigger the innate immune system (cells such as the complement system and macrophages) releasing powerful signals and enzymes that activate platelets and trigger blood clotting. These signals are interesting because they relate to processes in the body and therefore may directly participate in defense and repair systems and act as markers for disease. However, such process signals are also sensitive to the effects of blood sample preparation. Simply drawing blood from a vein through a needle or exposing blood to air can inadvertently activate these mechanisms. For example, altering the time, centrifugation speed, or temperature of sample processing steps can change the apparent composition of serum or plasma, and physiological information can be masked by preanalytical variability imparted to samples during collection and processing. The intense sensitivity of these processes and proteins to subtle changes in sample handling can compromise their use as biomarkers due to the associated lack of stability.

[0004] Currently, there is a strong interest in pre-analytical sample variation (often referred to as "batch effect") in biological research efforts using multivariate analysis. Currently, the quality of a sample can be judged primarily by visually obvious variations, such as red color indicating red blood cell lysis and cloudiness indicating high lipid or other contaminants. This allows clinicians to have limited confidence in all but the most robust and stable protein measurements. Ostroff, R. et al. (2010) J. Proteomics 73:649-666 reports a study that demonstrates some complex and nonlinear effects of variations in serum and plasma preparations. There, specific techniques are proposed to determine compliance with sample preparation protocols based on nonlinear (logarithmic) changes in measurements of a specific set of proteins that are affected by variations in the sample preparation protocol. Metrics derived from these methods can be used to monitor protocol compliance, reject samples, and correct for analytes of interest. These techniques are useful for assessing the quality of human or animal blood samples used in biomarker research, clinical diagnostic applications, quality monitoring of biobank samples, and drug development. Similar approaches can be developed to assess sample integrity for many other sample types, including urine, cerebrospinal fluid, sputum, or tissue. Summary of the Invention

[0005] As described herein, important characteristics of an ideal biomarker measurement necessary for biomarker discovery and to reach clinical utility include reliability and The reliability of a biomarker means that the signal of the biomarker is factual (i.e., it is not a "false positive" marker) in capturing the underlying biology of health or disease. The stability of a biomarker means that the biomarker is differentially expressed in diseased individuals compared to non-diseased individuals. To increase the probability of finding true disease biomarkers and reduce the variation of false positive identification due to sample bias, a method to measure sample quality and consistency is essential.

[0006] Measurements of protein analytes in plasma samples can be significantly affected by the protocols used to collect and handle the samples. Deviations from specified sample collection and / or handling protocols can result in altered protein levels in the sample or other systematic effects on measurements that result in altered signals for many analytes, including negative controls. Such deviations can occur regardless of the type of assay used to measure the protein analytes.

[0007] To assess the quality of a series of clinical samples, the impact of the most obvious protocol deviations was characterized. The change in protein composition as a function of time was assessed between sample collection and centrifugation. Additionally, the change in protein composition as a function of time was assessed between centrifugation of the sample and the time to decantation of the sample.

[0008] We identified signatures for sample mishandling that can be used as quantitative classifiers to assess clinical sample collection, and we developed metrics for each analyte that capture the sensitivity of that analyte's measurement to deviations from the collection protocol, particularly with respect to delays between sample collection and centrifugation, and between centrifugation and decanting of the sample.

[0009] One might imagine that some techniques are relatively unaffected by sample handling, but this is not true. Although antibodies work well in the presence of plasma and serum matrices, and mass spectrometry can measure peptides and even denatured proteins, if cells in a sample are lysed, or platelets are degranulated, or the complement system is activated, dramatic changes in the analyte concentration in the sample will occur after the sample is taken, and any "high fidelity" measurement technique would detect these changes. Thus, techniques similar to those described herein for determining the impact of sample handling variations may be useful for multiple assay formats and non-protein biomarkers. Although such assay formats may be sensitive in different forms, they may be subject to the same underlying causes of sample preparation variations.

[0010] It can be shown in a reproducible manner that variations in the various steps of blood handling and processing affect biological samples. The sensitivity of measurements of each biomarker protein to parameters related to the various sample handling and processing steps was quantified using SOMAmer® proteomics arrays to identify markers of variation in the sample handling process. Variations in sample handling and sample processing were quantified within the same assay measuring multiple analytes for obtaining measurements of disease biomarkers and for the developed method to determine which handling / processing markers were affected and approximately how much they were affected. The subject method also allows for setting limits on acceptable sample handling and sample processing quality metrics for biomarker discovery.

[0011] The following numbered items describe further aspects of the invention. 1. a) Levels of Sonic Hedgehog (SHH) protein in samples from human subjects measuring levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 2. The method of claim 1, wherein said measuring is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 3. The method of claim 1, wherein the sample is selected from blood, plasma, serum, or urine. 4. The method according to claim 1, comprising measuring SHH and PGAM1, SHH and PTPN4, SHH and TNFSF14, SHH and FAM49B, SHH and RBP7, SHH and IHH, SHH and DDX39B, SHH and S100A12, SHH and PGAM2, SHH and C4A·C4B, SHH and IL21R, SHH and TMEM9, or SHH and ADAM9. 5. SHH, PGAM1, and TNFSF14; SHH, PGAM1, and RBB7; SHH, PGAM1, and PTPN4; SHH, PGAM1, and DDX39B; SHH, PGAM1, and FAM49B; SHH, PGAM1, and IHH; SHH, PGAM1, and S100A12; SHH, PGAM1, and ADAM9; SHH, PTPN4, and RBP7; SHH, PTPN4, and TNFSF14; SHH, P TPN4 and IHH; SHH, RBP7 and FAM49B; SHH, RBP7 and IHH; SHH, FAM49B and TNFSF14; SHH, DDX39B and PTPN4; SHH, TNFSF14 and S100A12; SHH, IHH and RBP7; SHH, IHH and TNFSF14; SHH, RBP7 and TNFSF14; SHH, RBP7 and S100A12; SHH, RBP7 and DD X39B; SHH, TNFSF14, and DDX39B; SHH, S100A12, and DDX39B; SHH, FAM49B, and S100A12; SHH, IHH, and FAM49B; SHH, IHH, and DDX39B; SHH, TNFSF14, and ADAM9; SHH, FAM49B, and DDX39B; SHH, IHH, and ADAM9; SHH, PGAM1, and C4A·C4B; SHH, PGAM2, and The method of claim 1, comprising measuring RBP7; SHH, PGAM1, and IL21R; SHH, PGAM2, and PTPN4; SHH, PGAM2, and ADAM9, SHH, PGAM2, and C4A·C4B; SHH, PGAM2, and IL21R; SHH, IHH, and PGAM2; SHH, PGAM1, and PGAM2; SHH, TMEM9, and PGAM2, or SHH, TMEM9, and PGAM1. 6. The method according to claim 1, comprising measuring SHH and PGAM1, and at least two proteins selected from RBP7, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, IHH, PGAM2, C4A·C4B, IL21R, TMEM9, and ADAM9. 7. The method according to claim 1, comprising measuring SHH and IHH, and at least two proteins selected from RBP7, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, PGAM1, PGAM2, C4A·C4B, IL21R, TMEM9, and ADAM9. 8. The level of the protein is determined by measuring the level of the protein obtained by sampling the human subject and centrifuging the sample. 4. The method of any one of the preceding claims, wherein the method is used to predict the length of time between centrifugation of the sample and decanting of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 9. The method of claim 8, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 10. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having affinity for a different protein from a series of proteins including sonic hedgehog (SHH) and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of PGAM1, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, ADAM9, PGAM2, C4A·C4B, IL21R, and TMEM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 11. The method of claim 10, wherein the set of capture reagents is selected from aptamers, antibodies, and combinations of aptamers and antibodies. 12. The method according to claim 10, wherein the sample is selected from blood, plasma, serum, or urine. 13. The method according to claim 10, comprising measuring SHH and PGAM1, SHH and PTPN4, SHH and TNFSF14, SHH and FAM49B, SHH and RBP7, SHH and IHH, SHH and DDX39B, SHH and S100A12, SHH and PGAM2, SHH and C4A·C4B, SHH and IL21R, SHH and TMEM9, or SHH and ADAM9. 14. SHH, PGAM1, and TNFSF14; SHH, PGAM1, and RBB7; SHH, PGAM1, and PTPN4; SHH, PGAM1, and DDX39B; SHH, PGAM1, and FAM49B; SHH, PGAM1, and IHH; SHH, PGAM1, and S100A12; SHH, PGAM1, and ADAM9; SHH, PTPN4, and RBP7; SHH, PTPN4, and TNFSF14; SHH, PT PN4 and IHH; SHH, RBP7 and FAM49B; SHH, RBP7 and IHH; SHH, FAM49B and TNFSF14; SHH, DDX39B and PTPN4; SHH, TNFSF14 and S100A12; SHH, IHH and RBP7; SHH, IHH and TNFSF14; SHH, RBP7 and TNFSF14; SHH, RBP7 and S100A12; SHH, RBP7 and DDX 39B;SHH, TNFSF14, and DDX39B;SHH, S100A12, and DDX39B;SHH, FAM49B, and S100A12;SHH, IHH, and FAM49B;SHH, IHH, and DDX39B;SHH, TNFSF14, and ADAM9;SHH, FAM49B, and DDX39B;SHH, IHH, and ADAM9;SHH, PGAM1, and C4A·C4B;SHH, PGAM2, and R The method described in claim 10, comprising measuring BP7; SHH, PGAM1, and IL21R; SHH, PGAM2, and PTPN4; SHH, PGAM2, and ADAM9, SHH, PGAM2, and C4A·C4B; SHH, PGAM2, and IL21R; SHH, IHH, and PGAM2; SHH, PGAM1, and PGAM2; SHH, TMEM9, and PGAM2, or SHH, TMEM9, and PGAM1. 15. The method according to claim 10, comprising measuring at least two proteins selected from SHH and PGAM1, and RBP7, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, IHH, and ADAM9. 16. SHH and IHH, as well as RBP7, TNFSF14, PTPN4, and DDX 11. The method according to claim 10, comprising measuring at least two proteins selected from 39B, FAM49B, S100A12, PGAM1, and ADAM9. 17. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 18. The method of claim 17, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 19. a) measuring the levels of at least three, four, five, six, seven, or eight proteins selected from the group consisting of IHH, PTPN4, TNFSF14, FAM49B, RBP7, DDX39B, S100A12, and ADAM9 in a sample from a subject; b) distinguishing said sample as an analytical sample or a negative sample based on the levels of said three, four, five, six, seven, or eight proteins. Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 20. The method of claim 19, wherein the measuring is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 21. The method of claim 19, wherein the sample is selected from blood, plasma, serum, or urine. 22. IHH, RB7, and PTPN4;IHH, RB7, and TNFSF14;IHH, RB7, and FAM49B;IHH, RBP7, and DDX39B;IHH, RBP7, and S100A12;IHH, RB7, and ADAM9;IHH, TNFSF14, and PTPN4;IHH, TNFSF14, and FAM49B;IHH, TNFSF14, and DDX39B;IHH, TN 20. The method of claim 19, comprising measuring FSF14, and S100A12; IHH, TNFSF14, and ADAM9; IHH, FAM49, and PTPN4; IHH, FAM49, and TNFSF14; IHH, FAM49, and DDX39B; IHH, FAM49, and S100A12; IHH, ADAM9, and PTPN4, or IHH, FAM49, and ADAM9. 23. The method of any one of the preceding claims, further comprising measuring SHH and / or PGAM1. 24. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 25. The method of claim 19, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 26. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having affinity for a different protein from a series of proteins including 3, 4, 5, 6, 7, or 8 proteins selected from the group consisting of IHH, PTPN4, TNFSF14, FAM49B, RBP7, DDX39B, S100A12, and ADAM9; b) measuring the level of each of said series of proteins with said series of capture reagents; The method includes: 27. The method of claim 26, wherein the set of capture reagents is selected from aptamers, antibodies, and combinations of aptamers and antibodies. 28. The method according to claim 26, wherein the sample is selected from blood, plasma, serum, or urine. 29. IHH, RB7, and PTPN4; IHH, RB7, and TNFSF14; IHH, RB7, and FAM49B; IHH, RBP7, and DDX39B; IHH, RBP7, and S100A12; IHH, RB7, and ADAM9; IHH, TNFSF14, and PTPN4; IHH, TNFSF14, and FAM49B; IHH, TNFSF14, and DDX3 9B; IHH, TNFSF14, and S100A12; IHH, TNFSF14, and ADAM9; IHH, FAM49, and PTPN4; IHH, FAM49, and TNFSF14; IHH, FAM49, and DDX39B; IHH, FAM49, and S100A12; or IHH, FAM49, and ADAM9. 30. The method of any one of the preceding claims, further comprising measuring SHH and / or PGAM1. 31. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 32. The method of claim 26, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 33. a) measuring the level of at least one protein selected from RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and DDX39B and S100A12 in a sample from a human subject; b) distinguishing said sample as an analytical sample or a negative sample based on the level of RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and said at least one protein; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 34. The method of claim 33, wherein the measuring is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 35. The method of claim 33, wherein the sample is selected from blood, plasma, serum, or urine. 36. The method of claim 33, comprising measuring RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and S100A12; or RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and DDX39B. 37. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 38. The method of claim 33, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 39. a) Samples from human subjects were analyzed using the following capture reagents: RB7, FAM49B, contacting the subject with a series of capture reagents having affinity for different proteins from a series of proteins including at least one protein selected from TNFSF14, ADAM9, PGAM1, and DDX39B and S100A12; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 40. The method of claim 39, wherein the set of capture reagents is selected from aptamers, antibodies, and combinations of aptamers and antibodies. 41. The method of claim 39, wherein the sample is selected from blood, plasma, serum, or urine. 42. The method of claim 39, comprising measuring RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and S100A12; or RB7, FAM49B, TNFSF14, ADAM9, PGAM1, and DDX39B. 43. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 44. The method of claim 39, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 45. The method of any one of the preceding claims, wherein the level or levels of the protein are used to assign a quality score to the sample, and the quality score is then used to determine whether the sample is an analytical or non-analytical sample. 46. ​​The method of any one of the preceding items, wherein the level or levels of the protein are used to assign a quality score to the sample, which is then used to determine whether to use the sample for further analysis of additional proteins in the sample. 47. a) measuring the level of PGAM1 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 48. a) measuring the level of PGAM2 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM1, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 49. a) measuring the levels of C4A·C4B proteins and at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, PGAM1, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 50. a) measuring the level of PTPN4 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PGAM1, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 51. a) measuring the level of TNFSF14 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, PGAM1, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 52. a) measuring the level of FAM49B protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) The above SHH levels and the above types 1, 2, 3, 4, 5, 6, 7, and 8, distinguishing said sample as an analytical sample or a negative sample based on the levels of 9, 10, 11, 12, or 13 proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 53. a) measuring the level of RBP7 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 54. a) measuring the level of an IHH protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 55. a) measuring the level of DDX39B protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, IHH, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 56. a) measuring the level of S100A12 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, IHH, DDX39B, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 57. a) measuring the level of IL21R protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, IHH, S100A12, DDX39B, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 58. a) measuring the level of TMEM9 protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, IHH, S100A12, IL21R, DDX39B, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 59. a) measuring the level of ADAM9 protein and at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, PGAM1, FAM49B, RBP7, IHH, S100A12, IL21R, TMEM9, and DDX39B in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 60. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of Sonic Hedgehog (SHH) protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 61. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of PGAM1 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 62. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of PGAM2 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 63. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of C4A·C4B protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 64. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of PTPN4 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 65. a) A sample from a human subject was subjected to a test in which each capture reagent was capable of detecting the TNFSF14 protein, as well as SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9. b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 66. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of FAM49B protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 67. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of RBP7 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 68. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of an IHH protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, DDX39B, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 69. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of DDX39B protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, S100A12, IL21R, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 70. a) A sample from a human subject was subjected to a test in which each capture reagent was capable of detecting S100A12 protein, as well as SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, IL21R, TMEM9, and ADAM9. b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 71. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of IL21R protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, TMEM9, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 72. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of TMEM9 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, and ADAM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 73. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including levels of ADAM9 protein and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 proteins selected from the group consisting of SHH, PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, and TMEM9; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 74. a) measuring the level of Sonic Hedgehog (SHH) protein and the levels of at least one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins selected from the group consisting of PGAM1, PGAM2, C4A·C4B, PTPN4, TNFSF14, FAM49B, RBP7, IHH, DDX39B, S100A12, IL21R, TMEM9, and ADAM9 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said level of SHH and said levels of said one, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or thirteen proteins; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 75. a) measuring the levels of HNRNDPDL, PTPN4, PGAM2, C4A·C4B, EIF4A1, IHH, SHH, PGAM1, S100A9, and HLA·DRB3 in a sample from a human subject; b) identifying said sample as an analytical sample or a negative sample based on said levels of HNRNDPDL, PTPN4, PGAM2, C4A·C4B, EIF4A1, IHH, SHH, PGAM1, S100A9, and HLA·DRB3; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 76. The method of any one of the preceding claims, wherein said measuring is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 77. The method of any one of the preceding claims, wherein the sample is selected from blood, plasma, serum, or urine. 78. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 79. The method of any one of the preceding claims, wherein the time between taking the sample and centrifugation of the sample is about 0 hours to 0.5 hours, 0.5 hours to 1.5 hours, 1.5 hours to 3 hours, 3 hours to 9 hours, 9 hours to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 hours to 0.5 hours, 0.5 hours to 1.5 hours, 1.5 hours to 3 hours, 3 hours to 9 hours, 9 hours to 24 hours, or more than 24 hours. 80. a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having affinity for a different protein from a series of proteins including HNRNDPDL, PTPN4, PGAM2, C4A·C4B, EIF4A1, IHH, SHH, PGAM1, S100A9, and HLA·DRB3; b) determining the level of each of said set of proteins based on said set of capture reagents; The method includes: 81. The method of any one of the preceding claims, wherein the measuring is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 82. The method of any one of the preceding claims, wherein the sample is selected from blood, plasma, serum, or urine. 83. The method of any one of the preceding claims, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 84. The method of any one of the preceding claims, wherein the time between taking the sample and centrifugation of the sample is about 0 hours to 0.5 hours, 0.5 hours to 1.5 hours, 1.5 hours to 3 hours, 3 hours to 9 hours, 9 hours to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 hours to 0.5 hours, 0.5 hours to 1.5 hours, 1.5 hours to 3 hours, 3 hours to 9 hours, 9 hours to 24 hours, or more than 24 hours. 85. a) contacting a sample from a human subject with two capture reagents, one of which has affinity for the TMEM9 protein and the other of which has affinity for PGAM1; b) measuring the levels of each protein using the two capture reagents; The method includes: 86. a) measuring the levels of PGAM1 and TMEM9 in a sample from a human subject; b) classifying the sample as an analytical sample or a negative sample based on the levels of PGAM1 and TMEM9. and identifying the sample; The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 87. The method of paragraph 85 or paragraph 86, further comprising measuring the level of the IHH protein by a capture reagent having affinity for the IHH protein. 88. The method of claim 85 or 86, further comprising measuring the level of C4A·C4B protein by a capture reagent having affinity for C4A·C4B protein. 89. The method of paragraph 85 or paragraph 86, further comprising measuring the level of SHH protein with a capture reagent having affinity for SHH protein. 90. The method of paragraph 85 or 86, further comprising measuring the level of PGAM2 protein by a capture reagent having affinity for PGAM2 protein. 91. The method of paragraph 85 or 86, further comprising measuring the level of ADAM9 protein with a capture reagent having affinity for ADAM9 protein. 92. The method of paragraph 85 or 86, further comprising measuring the level of PTPN4 protein with a capture reagent having affinity for PTPN4 protein. 93. The method of paragraph 84 or paragraph 85, further comprising measuring the level of IL21R protein by a capture reagent having affinity for IL21R protein. 94. The method of paragraph 85 or 86, further comprising measuring the level of RBP7 protein by a capture reagent having affinity for RBP7 protein. 95. a) contacting a sample from a human subject with two capture reagents, one of which has affinity for the SHH protein and the other of which has affinity for IHH; b) measuring the levels of each protein using the two capture reagents; The method includes: 96. a) measuring the levels of SHH and IHH in a sample from a human subject; b) distinguishing said sample as an analytical sample or a negative sample based on said levels of SHH and IHH; The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 97. The method of paragraph 95 or paragraph 96, further comprising measuring the level of RBP7 protein by a capture reagent having affinity for RBP7 protein. 98. The method of paragraph 95 or paragraph 96, further comprising measuring the level of FAM94B protein with a capture reagent having affinity for FAM94B protein. 99. The method of paragraph 95 or paragraph 96, further comprising measuring the level of TNFSF14 protein by a capture reagent having affinity for TNFSF14 protein. 100. The method of claim 95 or 96, further comprising measuring the level of ADAM9 protein by a capture reagent having affinity for the ADAM9 protein. 101. The method of paragraph 95 or paragraph 96, further comprising measuring the level of S100A12 protein by a capture reagent having affinity for S100A12 protein. 102. The method of paragraph 95 or paragraph 96, further comprising measuring the level of DDX39B protein by a capture reagent having affinity for DDX39B protein. 103. The method of claim 95 or 96, further comprising measuring the level of PGAM1 protein by a capture reagent having affinity for PGAM1 protein. . 104. The method of paragraph 95 or paragraph 96, further comprising measuring the level of PTPN4 protein with a capture reagent having affinity for PTPN4 protein. 105. a) contacting a sample from a human subject with four capture reagents, each of the four capture reagents having an affinity for a protein selected from IHH, RBP7, ADAM9, and PTPN4; b) measuring the levels of each protein using the four capture reagents; The method includes: 106. The method of paragraph 105, further comprising measuring the level of SHH protein with a capture reagent having affinity for SHH protein. 107. The method of paragraph 105, further comprising measuring the level of PGAM1 protein by a capture reagent having affinity for PGAM1 protein. 108. The method of claim 105, further comprising measuring the level of one or more proteins selected from TMEM9, C4A·C4B, PGAM2, FAM49B, TNFSF14, S100A12, DDX39B, and IL21R with capture reagents, each capture reagent having affinity for one of the one or more proteins. 109. a) measuring the levels of IHH, RBP7, ADAM9, and PTPN4 proteins in a sample from a human subject; b) distinguishing said sample as an analytical sample or a negative sample based on the levels of IHH, RBP7, ADAM9, and PTPN4 proteins in said sample; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 110. The method of paragraph 109, further comprising measuring a level of SHH protein and distinguishing the sample as an analytical sample or a negative sample based on the level of SHH protein in the sample. 111. Measuring the level of PGAM1 protein; distinguishing said sample as an analytical sample or a negative sample based on said level of PGAM1 protein in said sample. 110. The method of claim 109, further comprising: 112. Measuring the level of one or more proteins selected from TMEM9, C4A·C4B, PGAM2, FAM49B, TNFSF14, S100A12, DDX39B, and IL21R; distinguishing said sample as an analytical sample or a negative sample based on said level of said one or more proteins. 110. The method of claim 109, further comprising: 113. a) contacting a sample from a human subject with four capture reagents, each of the four capture reagents having an affinity for a protein selected from IHH, RBP7, ADAM9, and PTPN4; b) measuring the level of each of the proteins in the sample by the four capture reagents; The method includes: 114. The method of paragraph 113, further comprising measuring the level of SHH protein with a capture reagent having affinity for SHH protein. 115. The method of paragraph 113, further comprising measuring the level of PGAM1 protein by a capture reagent having affinity for PGAM1 protein. 116. The method of paragraph 113, further comprising measuring the level of TMEM9 protein with a capture reagent having affinity for TMEM9 protein. 117. The method of claim 113, further comprising measuring the level of one or more proteins selected from C4A·C4B, PGAM2, FAM49B, TNFSF14, S100A12, DDX39B, and IL21R with capture reagents, each capture reagent having affinity for one of the one or more proteins. 118. The method of claim 113, wherein the sample is selected from blood, plasma, serum, or urine. 119. The method of claim 113, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 120. The method of claim 113, wherein the level of the protein is used to distinguish the sample as an analytical sample or a negative sample based on the level of the protein, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and wherein the negative sample is a sample not used as an analytical sample. 121. The method of claim 119, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 122. The method of paragraph 113, wherein the capture reagent is selected from an aptamer or an antibody. 123. a) measuring the levels of IHH, RBP7, ADAM9, and PTPN4 proteins in a sample from a human subject; b) distinguishing said sample as an analytical sample or a negative sample based on the levels of IHH, RBP7, ADAM9, and PTPN4 proteins in said sample; Including, The method, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample. 124. Measuring the level of SHH protein; distinguishing said sample as an analytical sample or a negative sample based on said level of SHH protein in said sample. 124. The method of claim 123, further comprising: 125. Measuring the level of PGAM1 protein; distinguishing said sample as an analytical sample or a negative sample based on said level of PGAM1 protein in said sample. 124. The method of claim 123, further comprising: 126. Measuring the level of TMEM9 protein; distinguishing said sample as an analytical sample or a negative sample based on said level of TMEM9 protein in said sample. 124. The method of claim 123, further comprising: 127. Measuring the level of one or more proteins selected from C4A·C4B, PGAM2, FAM49B, TNFSF14, S100A12, DDX39B, and IL21R; distinguishing said sample as an analytical sample or a negative sample based on said level of said one or more proteins. 124. The method of claim 123, further comprising: 128. The method of claim 123, wherein the sample is selected from blood, plasma, serum, or urine. 129. The level of the protein is determined by obtaining a sample from the human subject and centrifugation of the sample. 124. The method of claim 123, wherein the method is used to predict the length of time between centrifugation of the sample and decanting of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample. 130. The method of claim 129, wherein the time between taking the sample and centrifugation of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is about 0 to 0.5 hours, 0.5 to 1.5 hours, 1.5 to 3 hours, 3 to 9 hours, 9 to 24 hours, or more than 24 hours. 131. The method of paragraph 123, wherein said measuring said protein levels is carried out using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay. 132. The method of claim 123, wherein the levels of the proteins are used in a classifier selected from decision trees; bagging + boosting + forests; rule inference based learning; Parzen window; linear models; logistic; neural network methods; unsupervised clustering; K-means; hierarchical ascending / descending; semi-supervised learning; prototype methods; nearest neighbor methods; kernel density estimation; support vector machines; hidden Markov models; Boltzmann learning; and a random forest model is used with the levels of the proteins to distinguish the sample from an analytical sample or a negative sample. [Brief description of the drawings]

[0012] [Figure 1] FIG. 1 shows the RFU of PGAM1 versus time until centrifugation, with a significant change in signal observed with increasing time until centrifugation. [Diagram 2] FIG. 1 shows the RFU of analyte versus time to centrifugation, showing that the discriminatory features are very low. [Diagram 3] Figure 1 shows analyte importance in a model of time to centrifugation: after about 10 analytes, the relative importance of subsequent analytes decreases and reaches a steady state. [Figure 4] Figure 13 shows a decision tree for samples in the time to centrifuge model above. The first node splits samples based on TNFSF14 RFU, and if the RFU is greater than 756.2, it stops at a 24 hour prediction, otherwise it traverses further branches down. [Diagram 5] FIG. 1 shows the prediction error versus the number of trees in a random forest. [Figure 6] Figure 1 shows prediction error in a single-analyte random forest model: the horizontal and vertical lines indicate class thresholds, and the solid black line indicates the true predicted value line. [Figure 7]Figure 1 shows the stability of the Random Forest and Naive Bayes models: the Naive Bayes model shows continuous changes with shifting of the signal for a single analyte, whereas the Random Forest shows higher stability. [Figure 8] Figure 1. Model stability when scaling individual analytes. The true time to centrifugation, reported in the title of each plot, is compared to the predicted time when each analyte is scaled by its effect size. Each line represents the random forest prediction when the analyte in question is scaled and the remaining nine analytes are held constant. [Figure 9] FIG. 1 shows the cumulative analyte distribution function of the protein marker SHH for samples from 18 individuals with different times to centrifugation. [Figure 10] FIG. 1 shows the cumulative analyte distribution function of the protein marker IHH for samples from 18 individuals with different times to centrifugation. [Figure 11] FIG. 1 shows the cumulative analyte distribution function of the protein marker RBP7 for samples from 18 individuals with different times to centrifugation. [Figure 12] FIG. 1 shows the cumulative analyte distribution function of the protein marker FAM49B for samples from 18 individuals with different times to centrifugation. [Figure 13] FIG. 1 shows the cumulative analyte distribution function of the protein marker TNFSF14 for samples from 18 individuals with different times to centrifugation. [Figure 14] FIG. 1 shows the cumulative analyte distribution function of the protein marker ADAM9 for samples from 18 individuals with different times to centrifugation. [Figure 15] FIG. 1 shows the cumulative analyte distribution function of the protein marker S100A12 for samples from 18 individuals with different times to centrifugation. [Figure 16] FIG. 1 shows the cumulative analyte distribution function of the protein marker DDX39B for samples from 18 individuals with different times to centrifugation. [Figure 17]FIG. 1 shows the cumulative analyte distribution function of the protein marker PGAM1 for samples from 18 individuals with different times to centrifugation. [Figure 18] FIG. 1 shows the cumulative analyte distribution function of the protein marker PTPN4 for samples from 18 individuals with different times to centrifugation. [Figure 19] FIG. 1 shows analyte model performance, where the performance of each model was quantified using the RMSE of predicted time to centrifugation versus true time to centrifugation for each individual and sample time point. [Figure 20] FIG. 13 shows the poor performing analytes based on the percentage of times the analyte was used in each model's performance group to highlight the importance of each analyte to the model's performance. [Figure 21] FIG. 13 shows mid-performing analytes based on the percentage of times the analyte was used in each model's performance ensemble to reveal the importance of each analyte to model performance. [Figure 22] FIG. 1 shows top performing analytes based on the percentage of times the analyte was used in each model's performance pool to highlight the importance of each analyte to model performance. [Diagram 23] FIG. 1 shows the distribution of the number of models used with the specified number of analytes. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] Reference will now be made in detail to exemplary embodiments of the invention. While the invention will be described in conjunction with these enumerated embodiments, it will be understood that it is not intended that the invention be limited to these embodiments. Rather, the invention is intended to cover all alternatives, modifications, and equivalents that may be included within the scope of the present invention as defined by the claims.

[0014] One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention and are within the scope of the present invention, and the present invention is not limited to the methods and materials described.

[0015] Unless otherwise defined, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods, devices, and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods, devices, and materials are now described.

[0016] All publications, published patent documents, and patent applications cited in this application are indicative of the state of the art of the technical field(s) to which this application pertains. All publications, published patent documents, and patent applications cited herein are hereby incorporated by reference to the same extent as if each individual publication, published patent document, or patent application was specifically and individually indicated to be incorporated by reference.

[0017] In this application, including the appended claims, the singular forms "a," "an," and "the" include plural references unless the content clearly dictates otherwise, and are used interchangeably with "at least one" and "one or more." Thus, reference to an "aptamer" includes a mixture of multiple aptamers, reference to a "probe" includes a mixture of multiple probes, and so forth.

[0018] As used herein, the term "about" refers to a small increase or decrease in a numerical value that does not change the basic function of the item to which the numerical value is related.

[0019] As used herein, the terms "comprises," "comprising," "includes," "including," "contains," "containing," and any variations thereof, are intended to be non-exclusive inclusions such that a process, method, product-by-process, or composition that comprises, includes, or contains an element or set of elements may include not only those elements, but may also include other elements not expressly listed in or inherent to such process, method, product-by-process, or composition.

[0020] As used herein, "biomarker" refers to a target molecule that is indicative of or indicative of a normal or abnormal process in an individual, or of a disease or other condition in an individual. More specifically, a "biomarker" is an anatomical, physiological, biochemical, or molecular parameter that is associated with the presence of a particular physiological condition or process, whether normal or abnormal, and if abnormal, whether chronic or acute. Biomarkers can be detected and measured by a variety of methods, including laboratory assays and medical imaging. When a biomarker is a protein, expression of the corresponding gene can also be used as a surrogate measure of the amount or presence of the corresponding protein biomarker in a biological sample, or the methylation status of the gene encoding the biomarker or the protein that controls the expression of the biomarker.

[0021] The selection of biomarkers for a particular disease state first involves identifying markers that are measurable and statistically significant different in diseased populations compared to control populations for a particular medical use. Biomarkers can include molecules that easily diffuse, secrete, or shed into the bloodstream from diseased or diseased tissues in parallel with disease onset or progression, or from surrounding tissues and circulating cells in response to disease or disease. The identified biomarker or set of biomarkers has generally been clinically validated or has been shown to be a reliable indicator of the original intended use for which the biomarker was selected. Biomarkers can include a variety of molecules, including small molecules, peptides, proteins, and nucleic acids. Some important issues that affect the identification of biomarkers include data bias, including overfitting of available data and variations in sample handling protocols.

[0022] As used herein, "biomarker value," "value," "biomarker level," and "level" are used interchangeably to refer to a measurement obtained using any analytical method to detect a biomarker of interest in a biological sample and indicating the presence, absence, absolute amount or concentration, relative amount or concentration, titer, level, expression level, ratio of measured levels, etc., of or corresponding to the biomarker of interest in said biological sample. The exact nature of the "value" or "level" will depend on the particular design and components of the particular analytical method used to detect the biomarker of interest.

[0023] "Control range of a disease biomarker" or "control range of a biomarker" are used interchangeably and refer to the normal or non-diseased range of a biomarker in non-diseased or normal individuals, usually derived from a control population.

[0024] "Sample," "case," or "test set" are used interchangeably and refer to individuals or case patients who are suspected of being affected, i.e., may be affected, and who may ultimately be determined to be affected or not affected.

[0025] As used herein, "sample handling and sample processing markers," "handling / processing markers," "markers sensitive to variations in sample handling and processing protocols," "markers sensitive to pre-analytical variations," and the like, are used interchangeably to refer to markers that are known to be sensitive to variations in sample handling and sample processing protocols by the methods described herein. "Sample handling and sample processing markers" may or may not include biomarkers.

[0026] Markers of sample handling and processing can be identified from candidate markers in a control population of normal individuals. Candidate markers from samples obtained from the control population are analyzed to select candidate markers that are sensitive to variations in sample handling and processing protocols. Such variations include variations in sample processing time, processing temperature, storage time, storage temperature, composition of storage containers, and other storage conditions prior to sample assay; variations in the method used to draw samples from normal individuals, including, but not limited to, exposure of the sample to oxygen, needle gauge used for venipuncture, collection equipment, additives in collection tubes; variations in sample processing, including, but not limited to, centrifugation speed, centrifugation temperature and time, filtration and filter pore size; collection receptacle or container, freezing method, etc. Candidate markers identified as being substantially sensitive to variations are suitable as sample handling and processing markers. The candidate markers include a variety of molecules, including small molecules, peptides, proteins, and nucleic acids.

[0027] In some cases, it may be desirable to distinguish between selected handling / processing markers to exclude markers that may also be disease markers or markers for the particular disease of interest in the assay, whereas in cases where the number of handling / processing markers used is greater than, for example, about 20, 30, 50 or more, it may not be necessary to exclude handling / processing markers in such situations.

[0028] As used herein, "determining," "measurement," "detection," and the like, as used interchangeably herein, refer to the detection or quantification (measurement) of a molecule using any suitable method, including fluorescence, chemiluminescence, radiolabeling, surface plasmon resonance, surface acoustic wave, mass spectrometry, infrared spectroscopy, Raman spectroscopy, atomic force microscopy, scanning tunneling microscopy, electrochemical detection, nuclear magnetic resonance, quantum dots, etc. "Detect" and variations thereof refer to the identification or observation of the presence of a molecule in a biological sample and / or the measurement of the value of a molecule.

[0029] As used herein, the terms "biological sample", "sample" and "test sample" are used interchangeably herein to refer to any material, body fluid, tissue or cell obtained from an individual or otherwise derived from an individual. Such material, body fluid, tissue or cell includes blood (including whole blood, white blood cells, peripheral blood mononuclear cells, buffy coat, plasma, serum, dried blood spots collected on filter paper), sputum, tears, mucus, nasal washings, nasal aspirates, exhaled air, urine, semen, saliva, cyst fluid, cerebrospinal fluid, amniotic fluid, glandular fluid, lymphatic fluid, nipple aspirate, bronchial aspirate, pleural fluid, peritoneal fluid, synovial fluid, joint aspirate, ascites, cells, cell extracts and cerebrospinal fluid. Such material, body fluid, tissue or cell also includes all of the above experimentally isolated fractions. For example, a blood sample may be fractionated into serum or fractions containing specific types of blood cells, such as red blood cells or white blood cells (leukocytes). If desired, a sample may be a combination of multiple samples from an individual, such as a combination of tissue and fluid samples. The term "biological sample" also includes materials containing homogenized solid material, such as from a stool sample, tissue sample, or tissue biopsy. The term "biological sample" also includes materials from tissue or cell cultures. Any suitable method for obtaining a biological sample may be used, and exemplary methods include, for example, venisection, swab (e.g., buccal swab), lavage, fluid aspiration, and fine needle aspiration biopsy. Samples may also be obtained, for example, by microdissection (e.g., laser capture microdissection (LCM) or laser microdissection (LMD)), bladder washing, smear (e.g., PAP smear), or breast ductal lavage cytology. A "biological sample" obtained or derived from an individual includes such a sample which has been processed in any appropriate way after it has been obtained from the individual in question.

[0030] It will further be appreciated that the biological sample may be obtained by taking biological samples from multiple individuals and pooling them, or by pooling aliquots of each individual's biological sample.

[0031] "Cell Abuse" includes, but is not limited to, cell contamination, cell lysis, cell fragmentation, cell debris, internal cell components, and the like.

[0032] As used herein, "rejection of a sample" may refer to the rejection of a subset, group, or collection to which the sample belongs.

[0033] As used herein, "SOMAmer" or "Slow Off-Rate Modified Aptamer" refers to an aptamer with improved off-rate properties. SOMAmers can be generated using the modified SELEX method described in U.S. Publication No. 2009 / 0004667, entitled "Method of Producing Aptamers With Improved Off-Rate Properties," now U.S. Patent No. 7,947,447.

[0034] In this application, measurements of sample handling and sample processing marker proteins have been measured and found to have well-defined and reproducible behavior with respect to variations in sample collection and preparation.

[0035] The central idea here is to use some of the many process and handling marker proteins measurable in each sample to provide a graded response to variations in sample collection and sample preparation steps. In this sense, the signals of these handling / processing marker proteins can be used to monitor past events in blood sample processing, such as delays before centrifugation and delays before decantation. This is different from directly monitoring the degradation of the biomarker protein of interest, which is potentially more sensitive and more informative over a broad range. Using the methods described herein, the known sensitivity of the handling / processing markers to the respective process variations can be applied to the estimated value of a particular biomarker protein of interest to characterize the estimated quality of the sample in terms of post-collection changes of said biomarker. Monitoring of sample processing and sample handling markers can also be used to correct for the estimated effect of the respective variations of disease biomarkers by subtracting sample handling components from the apparent protein concentration. Measurements of these sample handling and sample processing biomarkers can be used to characterize samples prior to evaluation of disease biomarkers by various measurement systems, including antibody assays, mass spectrometry, etc.

[0036] In this way, some of the biological mechanisms of blood are used to function as a clock, timer, and recorder. For this technique to work, the in vivo biology of various mechanisms must be established. It is necessary to be able to distinguish between biological activation and activation that occurs after blood leaves the body, i.e., "in vitro" changes. The primary tool for distinguishing in vivo degradation of disease biomarkers and handling / processing markers from those that occur in vitro is the ability to measure a large number of proteins simultaneously so that the sample can be characterized for not just a single sample handling / processing variation, but several of such variations. Measurements of correlated proteins that indicate variation in a particular sample handling protocol provide a panel of sample handling / processing markers.

[0037] The metrics provided by our system for each sample allow for the elimination of a set of samples from a clinical site by evaluating a small number of samples to find that sample handling and processing techniques at one or more sites, or in some of the samples, may have made it difficult to measure differences in the biomarker proteins of interest. That is, the metrics allow for the determination of whether the sample in question will mask true health or disease biology due to sample handling effects, or whether sample handling effects will produce "false positive" biomarker results that do not actually reflect the underlying health or disease biology. The sample collection / processing metrics also provide a reliable and stable window of biomarker discovery. Selection of a group of samples with consistent sample preparation metrics can minimize unintended bias and enhance the discovery of disease-specific biomarkers. The metrics can also be used to correct for mild sample handling effects by comparing to well-collected standard samples. In clinical use, the sample handling metrics can be used to advise facilities on collection procedures, to eliminate some samples before costly extensive further evaluation, and to adjust the measurements or reports provided to reflect any uncertainties due to sample handling.

[0038] In short, this time, 1. It is possible to measure the form and quantify the degree of sample handling variability between samples, allowing for screening of a range of samples and identification of samples suitable for biomarker discovery. 2. Preferred sample handling / sample processing protocols can be identified or established to substantially reduce or minimize sample-to-sample variability. 3. Similarly, sample handling / sample processing values ​​for a collection facility or batch of samples can be compared to reference sample handling / processing biomarker values ​​to determine an individual facility's adherence to the preferred collection protocols described above. 4. A series of samples can be tested and compared to the handling / processing biomarker values ​​of reference samples to determine the degree of expected handling and processing variation that may exist between case and control samples. In this way, a subset of samples based on similar sample collection conditions can be selected and compared, so that the identified biomarkers will faithfully reflect the underlying biology. 5. An individual specimen may be excluded from use in diagnostic testing if it is determined that the specimen was not collected in accordance with good handling / processing protocols. 6. Protein measurements of one or more case samples may be adjusted to reflect variations in sample handling / sample processing. 7. A stable subset of proteins that are less sensitive to sample handling / processing variations can be selected for clinical or commercial use.

[0039] Thus, the present invention includes a method for quantifying the effect of deviations from ideal blood sampling conditions, the method including the collection and handling of blood samples prior to measurement of a proteomic assay. It involves identifying biological processes that are affected by the variation of the steps involved. These biological processes are monitored by a specific list of measurements of analytes (such as proteins) that are uniquely identified with such processes and can be monitored. These protein lists are quantitatively applied using predictions of logarithmic measurements of protein abundance using protein coefficients specific to each protein measured. The scores from these predictions, known as sample processing markers SMV (sample marker variability), can be used to assess procedural variations in blood sampling from sample to sample and from sample group to sample.

[0040] In one aspect, the invention protects a method for the creation of SMV coefficients. In particular, a method has been identified for quantifying the impact of deviations from ideal blood sampling conditions. The method involves the identification of biological processes that are affected by variations in the steps involved in the collection and handling of blood samples prior to the measurement of a proteomic assay. These biological processes are monitored by a specific list of protein measurements that are uniquely identified with such processes and can be monitored. These protein lists are quantitatively applied using log protein predictions of protein abundance measurements using protein coefficients specific to each protein measured. The scores from these predictions, known as sample processing markers SMV, can be used to assess procedural variations in blood sampling from sample to sample and from sample group to sample. These biological processes can be used to monitor variations in blood sampling conditions and specific protein vectors can be used to monitor and quantify such biological processes. This allows for the quantification of sample collection variations that are recorded on the sample itself and do not require independent monitoring of variables such as time, temperature, centrifugation speed, etc., during collection.

[0041] To identify SMV protein components, targeted experiments were used that involve biochemical manipulation of specific biological processes such as complement activation, platelet activation, and cell lysis. These experiments were combined with experiments that altered blood sampling conditions in a manner consistent with clinical practice to uniquely identify biological processes that can be used to quantitatively assess clinical sampling variability from sample to sample.

[0042] Using the techniques described herein, samples can be evaluated for the quality of measurements of proteins directly involved in these biological processes. This provides a quantitative measurement of sample quality that can be applied to give a judgment on the measurements of proteins in these samples that may be affected by sample handling variations but are not simply directly linked to the biological processes measured here. For example, general proteolytic activity may be affected by complement activation and cell lysis. However, the affected proteins do not form a simple closed group or process and cannot be used to monitor complement and cell lysis. This is because other proteins may have many reasons for variation between samples that are unrelated to sample handling variations, such as disease processes and renal function.

[0043] The use of a set of proteins with coefficients to monitor the biological process and indirectly the variation of the sampling conditions is an invention that has advantages over single proteins in that it forms an ensemble of measurements that are less likely to suffer from individual variations and can be interpreted to give a stable estimate of the activity of the biological process. The use of logarithmic scale measurements allows the monitoring of relative fold changes in the activity of the biological process, which can be easily compared to a reference sample using the difference corresponding to the ratio in linear space. This use of logarithm also implicitly scales the protein measurements such that different concentration ranges between proteins in a set or vector are automatically normalized when using a reference sample.

[0044] Direct application of SMV calculation to each blood sample will give a score that can be interpreted in terms of the biological process, or indirectly, the deviation of a particular sample collection condition from the ideal condition of a reference sample. These scores can then be used to clarify which samples meet the standard or are within acceptable limits. This information can be used to eliminate individual samples. In biomarker discovery, when changes in protein abundance may be caused by several sets of individual series of samples that are processed under different sample collection protocols or conditions, it is important to eliminate individual samples to avoid attributing such changes to the disease or process that is being investigated for the purpose of biomarker discovery.

[0045] The SMV scores for individual samples can be used to classify sets of samples that correspond to a particular range of sampling parameters. This allows a set of samples to be identified as matched sets of samples, where the samples in one set were sampled with the same procedure and have the same sampling parameters as the samples in the previous or another sampling study. This ability to form matched sets is very useful in comparisons between groups of samples that may have been sampled under different conditions. The SMV scores calculated from individual samples can also be used to correct for sample handling variations, if the associated variations in other proteins can be measured and a mathematical model can be constructed based on the variations in each protein that are affected by the processes that lead to the variations between samples with different SMV scores.

[0046] Rejecting individual samples based on their SMV scores allows for more sensitive biomarker discovery, since it is known that differences between samples taken from clinically different individuals refer to differences between those individuals, not differences in the method by which the samples were taken. Diagnostic tests involving protein abundance can be misleading when the variation is due to the procedure by which the blood sample was taken and not due to the clinical condition of the individual. This is avoided by rejecting samples that do not meet the SMV score threshold that corresponds to the variation of the reasonable sample collection procedure.

[0047] Many existing specimen collections are systematically damaged by variation in sample collection procedures. The SMV score can be used to quantify such variation within a specimen collection or between sampling sites, and can also be used to eliminate entire study samples based on variations that may mislead researchers, such as systematic variation in sample collection between cases and controls. If a specimen collection is deemed unacceptable, all that is needed to evaluate such variation is to measure only a subset of the collection, allowing for significant savings. It is also possible to monitor sample collection during the sample acquisition phase of a study, provide corrective recommendations, and detect non-compliance with study protocols. All that is needed to monitor variation in existing or ongoing study samples is to measure a small subsample of the entire collection.

[0048] These techniques for monitoring and evaluating sampling variability can be applied to optimizing research protocols and maximizing the economics of large-scale sampling efforts, such as biobanks, where the costs of using specialized sampling equipment and containers can be compared with accurate assessment of the variability and damage caused by operating less expensive protocols.

[0049] In some cases, perhaps due to the retrospective nature of most common biological specimen collections, pristine specimen collections are not available, and some comparisons may necessarily be made between specimens collected at different facilities and between groups of specimens collected at different times. These specimen collections will exhibit differences in collection procedures that will result in changes in proteomic profiles that may not be consistent with the intended outcome. This is confounded by differential clinical comparisons that involve the construction of matched sets between sample groups, allowing the comparison of equally collected subsets of samples.

[0050] Measurement of protein analytes in plasma samples can be significantly affected by the protocols used to collect and handle the samples. Deviations from the specified sample collection and / or handling protocols can lead to changes in protein levels within the sample or other systematic effects on the measurements that result in changes in the signal of many analytes, including negative controls. Such deviations can occur regardless of the type of assay used to measure the protein analytes.

[0051] To assess the quality of a series of clinical samples, the impact of the most obvious protocol deviations was characterized. The change in protein composition as a function of time was assessed between sample collection and centrifugation. Additionally, the change in protein composition as a function of time was assessed between centrifugation of the sample and the time to decantation of the sample.

[0052] We identified signatures for sample mishandling that can be used as quantitative classifiers to assess clinical sample collection, and we developed metrics for each analyte that capture the sensitivity of that analyte's measurement to deviations from the collection protocol, particularly with respect to delays between sample collection and centrifugation, and between centrifugation and decanting of the sample. EXAMPLES

[0053] The following examples are presented for illustrative purposes only and are not intended to limit the scope of the present application, which is defined by the appended claims. All examples described herein are carried out using standard techniques, which are well known and routine to those skilled in the art. The routine molecular biology techniques described in the following examples can be carried out as described in standard laboratory manuals, such as Sambrook et al., Molecular Cloning: A Laboratory Manual, 3rd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, (2001).

[0054] Example 1 Sample Collection Plasma samples were collected from a group of 18 individuals with all sampling variables, except for the variables of interest, held constant to a defined protocol. To assess the variability of responses between different individuals, blood was collected in multiple collection tubes from the same set of individuals. Sampling steps 1. I checked the expiration date of the EDTA tube with a light purple cap. If it had expired, I replaced it with a new one. 2. The above-mentioned EDTA tubes with light purple caps were properly labeled with the participant ID and blood collection date. 3. Venipuncture was performed according to institutional guidelines in compliance with standard health and safety procedures. 4. The above EDTA tube with a light purple cap was filled to the brim with the sample from the venipuncture. 5. When collecting multiple blood samples at the same time, the blood samples in each blood collection tube should be collected in the following order: a.For serum b.Citrate / plasma c.Heparin / plasma d. EDTA tube with light purple cap / for plasma The samples were taken at . 6. Invert the EDTA tube with the light purple cap 8 to 10 times and place it upright on the rack. Placed it.

[0055] Time to centrifuge Samples were collected in vacutainer tubes and inverted as described above in "Sample Collection-Step". Six different times were then allowed to elapse for each of the 18 individual samples before the samples were centrifuged: 0, 0.5, 1.5, 3, 9, and 24 hours. The EDTA tubes with light purple caps were centrifuged at 2200 x g (not RPM) for 15 minutes. The microtubes were properly labeled with the participant ID. 1.0 ml of plasma was pipetted into a microtube. Only the plasma layer was withdrawn. Care was taken not to disturb the buffy coat when withdrawing the aliquots by leaving some plasma and avoiding the cell layer. The tops of the microtubes were closed and placed in a -80°C freezer.

[0056] Time to decant / freeze Samples were collected by centrifugation of the samples as described above under "Sample Collection-Steps" and "Time to Centrifugation". Six different times were then allowed to elapse for each of the 18 individual samples, namely 0, 0.5, 1.5, 3, 9, and 24 hours, before the spun down samples were decanted and frozen.

[0057] Univariate analysis Prior to model creation, univariate analysis of all analyte signals with respect to time to centrifuge / time to decant was performed to reduce the number of features (analytes) used for model building (Figures 1-2).

[0058] Pearson correlations (Equation 1) of the RFUs of the samples from these 18 individuals were calculated for each of the approximately 5K analytes to provide information on the overall functional impact of variations in time to centrifuge / time to decant.

number

[0059] Although there is a range of behavior, the analytes can be characterized as either highly discriminatory with Pearson correlation coefficients >0.95 (Figure 1) or very low discriminatory with correlation coefficients <1E-3 (Figure 2) that show virtually no change with time to centrifugation in samples from 18 individuals.

[0060] Summary statistics for a few analytes highly correlated with time to centrifuge / time to decant are shown in Tables 1 and 2. Table 3 ranks the importance of analytes to time to centrifuge / time to decant. There are qualitative groups of analytes in the time to centrifuge model, i.e., groups that show a negative or positive correlation shift in RFU with increasing time to centrifuge, and groups that have different degrees of response to time to centrifuge. Table 4 shows the correlation between measured analyte levels and time to centrifuge (e.g., measured levels of SHH decrease as time from collection to centrifugation increases (negative correlation)).

[0061] Using the calculated analyte correlations, the number of potential markers that may be useful for classifying time to centrifuge / time to decant was reduced from approximately 5K to approximately 100 with calculated Pearson correlations >|0.7|. This initial set of analytes was used for further feature reduction in building the classifier.

[0062] Example 2: Creation of a classifier A random forest classifier was chosen to create the sample handling model. A brief introduction to random forest, its implementation using SOMAscan data, and its advantages over alternative machine learning techniques are presented below.

[0063] Random forest model Briefly, a random forest is a collection of many (hundreds) of decision trees, such as the example below (Figure 4): The RFU level at a node will split the tree in two directions, either leading to an endpoint and classification prediction, or leading to another node (where further analyte RFU values ​​will split the tree again, leading to multiple branches further down).

[0064] The advantage of random forests is that while a single decision tree is prone to prediction errors such as the multiple incorrect discretizations in Figure 4, averaging over hundreds of trees reduces the error for any given prediction (Figure 5).

[0065] Creating the model Random forest models were modeled using the Caret (Kuhn, M. (2008). Caret package. Journal of Statistical Software, 28(5)) and randomForest (A. Liaw and M. Wiener (2002). Classification and Regression by randomForest. R News 2(3), 18-22) packages in the R language, with log 10 The transformed RFU data was trained. Further feature reduction was performed by assessing IncNodePurity (Gini coefficient in classification), a measure of the relative importance of each analyte to the model's performance. From here, we further reduced the features to create a model that included the 10 most important analytes in time to centrifuge / time to decant (Table 3, Figure 3).

[0066] When assessing the performance of the model using individual analytes (Figure 6; PGAM1 model), two metrics are used: first, the evaluation of the predicted time against the true time using the root mean square error (RMSE), or thresholding the time of the sample according to what is considered a properly collected sample (true time to centrifuge / decant <2 hours) or what is considered an improperly collected sample (true time >2 hours). Using this binary classification, the predicted value can be assigned as true positive (TP), meaning that the predicted time correctly represents a properly collected sample, true negative (TN), meaning that the predicted time correctly represents an improperly collected sample, and the cross terms false positive (FP) and false negative (FN). Using only PGAM1 as a predictor of time to centrifuge (Figure X), a good level of sensitivity / specificity is observed for the above binary classification system. However, longer times to centrifuge often underestimate or overestimate the true value.

[0067] Model Stability An additional advantage of the Random Forest model is its robustness in the presence of noise in the assay. Considering a sample with a true centrifugation time of 9 hours (FIG. 7), the predictions of this model are more robust (solid curve) than the analogous Naive Bayes model (dashed curve) when the RFU values ​​for a single analyte (SHH in this example) are scaled by some effect size.

[0068] For a given sample and time to centrifugation, When the RFUs are adjusted independently, it is observed that there are relatively stable points above which the actual prediction does not change significantly (Figure 8). For more extreme changes in the analyte signal, small jumps in prediction time are observed rather than a continuous change.

[0069] Binary Classification Performance Using a predefined cutoff time of 2 hours, samples with actual centrifuge / decant times less than 2 hours as "good" samples, and samples with actual centrifuge / decant times greater than 2 hours as "problematic / bad" samples, the overall sensitivity and specificity were determined for each model's predicted value for what was known to be the actual class. Using this binary classification, the predicted value was assigned as true positive (TP), meaning the predicted time correctly represents a properly collected sample, true negative (TN), meaning the predicted time correctly represents an improperly collected sample, and cross terms, false positive (FP), meaning improperly collected samples, and false negative (FN), meaning properly collected samples. For example, the binary classification in Table 5 was performed based on the PGAM1 model (Figure 6) at actual times of 1.5 hours or 3 hours.

[0070] The confusion matrix contained the following information: [Table 1]

[0071] Seventeen samples were classified as true positives, 15 as true negatives, 1 as false negatives, and 3 as false positives.

[0072] The sensitivity of the model is

number

number

[0073] For these 18 individual samples at two time points, the sensitivity / specificity was

number

[0074] Sensitivity / specificity across 18 individual samples is calculated at the six different times to centrifuge / time to decant.

[0075] Calculating RMSE The root mean square error (RMSE) is a continuous measure of performance calculated in terms of time to true centrifugation versus prediction at each sample and time.

number

[0076] For the 18 individual samples with an actual time to centrifugation of 9 hours in the example of the PGAM1 marker, the numerator of this formula contains the data from Table 6.

[0077] By adding up the squared differences and using N=18 samples, the above formula becomes:

number

[0078] Reducing the difference between the predicted time and the actual time reduces the RMSE and is therefore a good indicator of the model's performance. An RMSE of 0 across all time points and samples corresponds to each prediction being equal to the actual time to centrifugation (i.e., a perfect predictor).

[0079] Model training and performance A sample of 18 individuals was used to train the model and evaluate the model's performance. A two-class system was created by defining "good" samples as those with a predicted time to centrifugation less than 2 hours. Furthermore, the relative error relating the predicted time to the actual time is a further indicator of the model's performance (RMSE). The random forest model limits the predictions to 0-24 hours (where data is available). Figure 6 shows the performance of the model using a single analyte predictor, with the predictor colored according to whether the sample was correctly identified as a true positive, which is a sample with a correctly predicted time to centrifugation less than 2 hours, and the alternative class of predictors, plotted against the true time to centrifugation as an indicator of the model's accuracy.

[0080] Cumulative distribution functions of samples from 18 individuals with different times to centrifugation are shown in Figures 9 to 18 .

[0081] Analyte model performance Analyses to quantify the performance of individual analytes and analyte combinations are summarized in Tables 7-24. Table 7 shows the time to centrifugation for quantification of the performance of the single marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 8 shows the time to centrifugation for quantification of the performance of the two marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 9 shows the time to centrifugation for quantification of the performance of the three marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 10 shows the time to centrifugation performance of the model using Sonic Hedgehog (SHH) for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 11 shows the time to centrifuge performance of the model using Indian Hedgehog (IHH) for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 12 shows the time to centrifuge performance of the model using ADAM9 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 13 shows the time to centrifuge performance of the model using DDX39B for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 14 shows the time to centrifuge performance of the model using FAM49B for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 15 shows the time to centrifugation performance of the model using PGAM1 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation).Table 16 shows the time to centrifuge performance of the model using PTPN4 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 17 shows the time to centrifuge performance of the model using RBP7 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 18 shows the time to centrifuge performance of the model using S100A12 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 19 shows the time to centrifuge performance of the model using TNFSF14 for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 20 shows the time to decant for quantification of the performance of the single marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 21 shows the time to decant for quantification of the performance of the two-marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 22 shows the time to decant for quantification of the performance of the two-marker model for all predicted time points (samples left for 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 23 shows the time to decant performance of the model using the combination of IHH, RBP7, ADAM9, and PTPN4 for all predicted time points (samples left 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation). Table 24 shows the time to centrifugation performance of the model using the combination of analytes, some of which include PGAM1 and / or PTPN4, for all predicted time points (samples left 0, 0.5, 1.5, 3, 9, and 24 hours before centrifugation).

[0082] Analyte model clustering The performance of each model was quantified using the RMSE of the predicted time to centrifugation versus the true time to centrifugation for each individual and sample time point. Figure 19 shows the distribution of RMSE values ​​for the 1023 models. This distribution separates four groups of model performance: high performing models with RMSE between 0 and 0.35, intermediate performing models with RMSE between 0.35 and 0.5, poor performing models with RMSE between 0.5 and 1, and very poor performing models with RMSE between 1 and 2.

[0083] Analytes used As shown in Figures 20-22, the percentage of times an analyte was used in each group of model performance was quantified to reveal the importance of each analyte to model performance.

[0084] Analyte grouping on model performance The distribution of the number of analytes required for high-performing models is shown in Figure 23. It is possible that a well-performing model uses as few as two analytes for all analytes.

[0085] Example 3 Multiplexed aptamer analysis of samples This example describes a multiplexed aptamer assay that was used to analyze samples and controls to identify the sampling / sample processing variability markers shown in Table 1.

[0086] Multiplexed aptamer assay method All steps of the multiplex aptamer assay were carried out at room temperature unless otherwise indicated.

[0087] Preparation of aptamer mixture stock solution The 5272 aptamers were grouped into three distinct mixtures, Dil1, Dil2, and Dil3, corresponding to 20%, 0.5%, and 0.005% dilutions of plasma or serum samples, respectively. The allocation of aptamers into mixtures was determined empirically by analyzing dilution series of plasma and serum samples combined with each aptamer and identifying the sample dilution that gave the largest linear range signal. A total of 10 aptamers were obtained by distributing and mixing the aptamers with different dilutions (20%, 0.5%, or 0.005%) of plasma or serum samples. 7 This allows the assay to span a 2-fold range of protein concentrations. Aptamer mixture stock solutions were prepared in HE-Tween buffer (10 mM Hepes, pH 7.5, 1 mM EDTA, 0.05% Tween 20) at a concentration of 4 nM of each aptamer and stored frozen at -20°C. 4271 aptamers were mixed in Dil1 mixture, 828 aptamers in Dil2, and 173 aptamers in Dil3 mixture. Prior to use, the stock solutions were diluted with HE-Tween buffer to a working concentration of 0.55 nM of each aptamer and dispensed into individual working aliquots. The aptamer mixture stock solutions were used in the Catch-0 primers. Prior to use in plate preparation, the working solution was heated / cooled by incubating at 95° C. for 10 min and then at 25° C. for at least 30 min to refold the aptamer.

[0088] Preparation of Catch-0 plates 60 μL of a 10% slurry of Streptavidin Mag Sepharose (GE Healthcare, 28-9857) was dispensed into each well of a 96-well plate (Thermo Scientific, AB-0769). The beads were then diluted with 175 μL of assay buffer (40 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM KCl, 5 mM MgCl 2The plates were washed once with 1 mM EDTA, 1 mM Tween-20) and then 100 μL of the heated / cooled aptamer mixture stock solution was added to each well. The plate was incubated at 25° C. for 30 min with shaking at 850 rpm on a ThermoMixer C shaker (Eppendorf). After the 30 min incubation, 6 μL of MB Block buffer (50 mM D-biotin in 50 mM Tris-HCl, pH 8, 0.01% Tween) was added to each well of the plate and the plate was incubated for an additional 2 min with shaking. The plate was then washed with 175 μL of assay buffer on a ThermoMixer C with a 1 min wash cycle with shaking at 850 rpm, followed by separation on a magnet for 30 s. After removing the wash solution, the beads were resuspended in 175 μL of assay buffer and stored at −20° C. until use.

[0089] Preparation of Catch-2 beads Prior to starting the robotic processing of the assay, a 10 mg / mL bead slurry of MyOne Streptavidin C1 beads (Dynabeads, product no. 35002D, Thermo Scientific) used in the Catch-2 step of the multiplex aptamer assay was washed in bulk with MB Prep buffer (10 mM Tris-HCl, pH 8, 1 mM EDTA, 0.4% SDS) for 5 minutes, followed by two washes with assay buffer. After the final wash, the beads were resuspended at a concentration of 10 mg / mL, and 75 μL of bead slurry was dispensed into each well of the Catch-2 plate. At the start of the assay, the Catch-2 plate was placed into the aluminum adaptor and placed in the appropriate position on the Fluent deck.

[0090] Thawing and dilution of samples A 65 μL aliquot of 100% plasma or serum samples stored in 80° C. Matrix tubes was thawed by incubating at room temperature for 10 minutes. To facilitate thawing, the tubes were placed on top of a fan unit that circulated air through the Matrix tube rack. After thawing, the samples were centrifuged at 1000×g for 1 minute and placed on the deck of the Fluent robot to dilute the samples. 20% sample solutions were prepared by transferring 35 μL of thawed sample into a 96-well plate containing 140 μL of the appropriate sample diluent. Sample diluent for plasma was 50 mM Hepes, pH 7.5, 100 mM NaCl, 8 mM MgCl 2 , 5 mM KCl, 1.25 mM EGTA, 1.2 mM Benzamidine, 37.5 μM Z-Block, and 1.2% Tween-20. Serum sample dilutions contained 75 μM Z-Block, and other components were at the same concentrations as the plasma sample dilutions. Dilutions in assay buffer to make 0.5% and 0.005% diluted samples were then performed using serial dilutions on the Fluent robot. To make the 0.5% sample dilution, an intermediate dilution from the 20% sample to 4% was made by mixing 45 μL of the 20% sample with 180 μL of assay buffer, and then the 0.5% sample was made by mixing 25 μL of the 4% diluted sample with 175 μL of assay buffer. To make the 0.005% sample, mix 20 μL of the 0.5% sample with 180 μL of assay buffer to make a 0.05% intermediate dilution, then mix 20 μL of the 0.05% sample with 180 μL of assay buffer. Assay buffer was mixed to prepare a 0.005% sample.

[0091] Sample binding step Catch-0 plates were prepared by immobilizing the aptamer mixture on streptavidin magnetic sepharose beads as described above. Frozen plates were thawed at 25°C for 30 min and washed once with 175 μL of assay buffer. 100 μL of each sample dilution (20%, 0.5%, 0.005%) was added to the plate containing beads containing the three different aptamer mixture stock solutions (Dil1, Dil2, Dil3, respectively). The Catch-0 plate was then sealed with an aluminum foil seal (Microseal 'F' Foil, Bio-Rad) and placed in a four-plate rotary shaker (PHMP-4, Grant Bio) set at 850 rpm and 28°C. The sample binding step was carried out for 3.5 h.

[0092] Multiplexed aptamer assay processing on the Fluent robot After the sample binding step was completed, the Catch-0 plate was placed in an aluminum plate adaptor and placed on the deck of the robot. A temperature controlled plate was used to perform the magnetic bead washing step. The plate was set at a temperature of 25° C. for all robotic processing steps, except for the Catch-2 wash described below. The plate was washed four times with 175 μL of assay buffer, with each wash cycle programmed to shake the plate at 1000 rpm for at least 1 minute, followed by separation of the magnetic beads for at least 30 seconds, after which the buffer was aspirated. During the final wash cycle, 100× Tag reagent (EZ-Link NHS-PEG 4The Tag reagent was prepared by diluting 1:100 of 100 mM of 100-biotin, product no. 21363, Thermo, prepared in anhydrous DMSO) in assay buffer and injected into a trough on the deck of the robot. 100 μL of Tag reagent was added to each well of the plate and incubated for 5 minutes with shaking at 1200 rpm to biotinylate the proteins captured on the bead surface. The biotinylation reaction mixture was quenched by adding 175 μL of quench buffer (20 mM glycine in assay buffer) to each well. The plate was left to incubate for 3 minutes and then washed 4 times with 175 μL of assay buffer, the washes being performed under the same conditions as above.

[0093] Photocleavage and kinetic challenges After the final wash of the plate, add 90 μL of photocleavage buffer (2 μM of oligonucleotide competitive inhibitor in assay buffer; this competitive inhibitor is 5'-(AC-Bn-Bn) 7 A nucleotide sequence of -AC-3' (where Bn is a benzyl-substituted deoxyuridine residue at position 5) was added to each well of the plate. The plate was transferred to the photocleavage accessory on the deck of the Fluent. The accessory consists of a BlackRay light source (UVP XX-Series Bench Lamps, 365 nm) and three Bioshake 3000-T shakers (Q Instruments). The plate was irradiated for 20 minutes with shaking at 1000 rpm.

[0094] Catch-2 bead capture At the end of the photocleavage process, the buffer was removed from the Catch-2 plate by magnetic separation and the plate was washed once with 100 μL of assay buffer. The photocleaved eluate containing the aptamer-protein complex was drawn from each Catch-0 plate, starting with the dilution 3 plate. All 90 μL of this solution was first transferred to the Catch-1 eluate plate, which was placed on the shaker with the magnet elevated, to trap any streptavidin magnetic sepharose beads that may have been aspirated. The solution was then transferred to the Catch-2 plate and incubated for 3 minutes at 25° C. while the plate was shaken at 1400 rpm. After this 3 minute incubation, the magnetic beads were separated for 90 seconds, the solution was removed from the plate, and the photocleaved Dil2 plate solution was added to the plate. Following the same process, the solution from the Dil1 plate was added and incubated for 3 minutes. At the end of this 3 min incubation, 6 μL of MB block buffer was added to the magnetic bead suspension and incubated at 25° C. for 2 min while shaking the beads at 1200 rpm. After this incubation, the plate was transferred to another shaker preset at a temperature of 38° C. The solution was removed after 2 min separation of the magnetic beads. The Catch-2 plate was then washed 4 times with 175 μL of MB wash buffer (20% glycerol in assay buffer), with each wash cycle programmed to shake the beads at 1200 rpm for 1 min and separate the beads on the magnet for 3.5 min. During the last bead separation step, the shaker temperature was set to 25° C. The beads were then washed once with 175 μL of assay buffer. For this wash step, the beads were shaken at 1200 rpm for 1 min and then separated on the magnet for 2 min. This wash step was followed by elution buffer (1.8 M NaClO 4Aptamers were eluted from the purified aptamer-protein complexes using 1000 mM PBS (pH 6.8, 40 mM PIPES, pH 6.8, 1 mM EDTA, 0.05% TritonX-100). Elution was performed with 75 μL of elution buffer at 25°C for 10 min while shaking the beads at 1250 rpm. 70 μL of the eluate was transferred to an archive plate and separated on a magnet to separate any potentially aspirated magnetic beads. 10 μL of the eluate was transferred to a black half-area plate, diluted 1:5 with assay buffer, and used to measure the Cy3 fluorescent signal monitored as an internal assay quality control. 20 μL of the eluate was transferred to a plate containing 5 μL of hybridization blocking solution (Oligo aCGH / ChIP-on-chip Hybridization Kit, large capacity, Agilent Technologies 5188-5380, containing spikes of cyanine 3-labeled DNA sequences complementary to the corner marker probes on the Agilent array). The plate was removed from the robot deck and further processed for hybridization (see below). The archive plate with the remaining eluate was heat sealed in aluminum foil and stored at -20°C.

[0095] Hybridization 25 μL of 2× Agilent hybridization buffer (Oligo aCGH / ChIP-on-chip Hybridization Kit, Agilent Technologies, Part No. 5188-5380) was manually pipetted into each well of the plate containing the eluted samples and blocking buffer. 40 μL of this solution was manually pipetted into each “well” of a hybridization gasket slide (Hybridization Gasket Slide—8 microarrays per slide format, Agilent Technologies). Custom SurePrint G3 8×60k Agilent microarray slides containing 10 probes per array complementary to each aptamer were placed on the gasket slide according to the manufacturer’s protocol. Each assembly (Hybridization Chamber Kit—SureHyb compatible, Agilent Technologies) was clamped tightly and placed in a hybridization oven at 55° C. and 20 rpm for 19 hours.

[0096] Post-hybridization washes Slide washing was performed using a Little Dipper Processor (Model 650C, Scigene). Approximately 700 mL of Wash Buffer 1 (Oligo aCGH / ChIP-on-chip Wash Buffer 1, Agilent Technologies) was poured into a large glass staining dish and used to separate the microarray slide from the gasket slide. Once disassembled, the slides were quickly transferred into a slide rack in a bath containing Wash Buffer 1 on the Little Dipper. The slides were washed in Wash Buffer 1 for 5 minutes while being mixed by a magnetic stir bar. The slide rack was then transferred to a bath containing Wash Buffer 2 (Oligo aCGH / ChIP-onchip Wash Buffer 2, Agilent Technologies) at 37°C and incubated for 5 minutes with agitation. The slide rack was slowly removed from the second bath and transferred to a bath containing acetonitrile and incubated for 5 minutes with agitation.

[0097] Microarray imaging Microarray slides were imaged using a microarray scanner (Agilent G4900DA Microarray Scanner System, Agilent Technologies) at 100% PMT setting, 3 μm resolution, and 20-bit option enabled in the Cyanine 3 channel. The resulting tiff images were processed using Agilent Feature Extraction software (version 10.7.3.1 or later) with the GE1_1200_Jun14 protocol.

Claims

1. 1. A method for evaluating sample handling and / or sample processing, comprising: a) measuring the level of an IHH protein and at least one protein selected from the group consisting of PGAM1, PGAM2, C4A-C4B, PTPN4, TNFSF14, FAM49B, RBP7, DDX39B, S100A12, SHH, IL21R, TMEM9, and ADAM9 in a sample from a human subject, wherein the sample is selected from blood, plasma, or serum; b) distinguishing said sample as an analytical sample or a negative sample based on the level of said IHH and said level of said at least one protein; Including, The method according to the present invention, wherein the analytical sample is a sample used for one or more of a protein biomarker discovery analysis, a protein expression level analysis, and a diagnostic or prognostic method, and the negative sample is a sample not used as an analytical sample.

2. The method of claim 1 , wherein the measuring is performed using mass spectrometry, an aptamer-based assay, and / or an antibody-based assay.

3. The method of claim 1 or 2, comprising measuring IHH and PGAM2, IHH and C4A / C4B, IHH and PTPN4, IHH and TNFSF14, IHH and PGAM1, IHH and FAM49B, IHH and RBP7, IHH and DDX39B, IHH and S100A12, IHH and IL21R, IHH and TMEM9, or IHH and ADAM9.

4. IHH, PGAM1, and PGAM2; IHH, PGAM1, and C4A and C4B; IHH, PGAM1, and PTPN4; IHH, PGAM1, and TNSF14; IHH, PGAM1, and FAM49B; IHH, PGAM1, and RBP7; IHH, PGAM1, and DDX39B; IHH, PGAM1, and S100A12; IHH, PGAM1, and IL21R; IHH, PGAM1, and TMEM9; IHH, PGAM1, and ADAM9; IHH, PGAM2, and C4A and C4B; IHH, PGAM2, and PTPN4; IHH, PGAM 2, and TNSF14; IHH, PGAM2, and FAM49B; IHH, PGAM2, and RBP7; IHH, PGAM2, and DDX39B; IHH, PGAM2, and S100A12; IHH, PGAM2, and IL21R; IHH, PGAM2, and TMEM9; IHH, PGAM2, and ADAM9; IHH, C4A·C4B, and PTPN4; IHH, C4A·C4B, and TNSF14; IHH, C4A·C4B, and FAM49B; IHH, C4A·C4B, and RBP7; IHH, C4A·C4B, and DDX39B; IHH, C4A· C4B, and S100A12; IHH, C4A·C4B, and IL21R; IHH, C4A·C4B, and TMEM9; IHH, C4A·C4B, and ADAM9; IHH, PTPN4, and TNFSF14; IHH, PTPN4, and FAM49B; IHH, PTPN4, and RBP7; IHH, PTPN4, and DDX39B; IHH, PTPN4, and S100A12; IHH, PTPN4, and IL21R; IHH, PTPN4, and TMEM9; IHH, PTPN4, and ADAM9; IHH, TNFSF14, and FAM49B; IHH, TN FSF14, and RBP7; IHH, TNFSF14, and DDX39B; IHH, TNFSF14, and S100A12; IHH, TNFSF14, and IL21R; IHH, TNFSF14, and TMEM9; IHH, TNFSF14, and ADAM9; IHH, FAM49B, and RBP7; IHH, FAM49B, and DDX39B; IHH, FAM49B, and SA100A12; IHH, FAM49B, and IL21R; IHH, FAM49B, and TMEM9; IHH, FAM49B, and ADAM9; IHH, RBP7, and DDX39B;IHH, RBP7, and S100A12; IHH, RBP7, and IL21R; IHH, RBP7, and TMEM9; IHH, RBP7, and ADAM9; IHH, DDX39B, and S100A12; IHH, DDX39B, and IL21R; IHH, DDX39B, and TMEM9; IHH, DDX39B, and AD 3. The method of claim 1 or 2, comprising measuring: IHH, S100A12, and IL21R; IHH, S100A12, and TMEM9; IHH, S100A12, and ADAM9; IHH, IL21R, and TMEM9; IHH, IL21R, and ADAM9; or IHH, TMEM9, and ADAM9.

5. The method of claim 1 or 2, comprising measuring at least two proteins selected from IHH and PGAM1, and RBP7, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, SHH, PGAM2, C4A / C4B, IL21R, TMEM9, and ADAM9.

6. The method of claim 1 or 2, comprising measuring at least two proteins selected from IHH and RBP7, and SHH, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, PGAM1, PGAM2, C4A / C4B, IL21R, TMEM9, and ADAM9.

7. 7. The method of any one of claims 1 to 6, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample.

8. 8. The method of claim 7, wherein the time between taking the sample and centrifugation of the sample is between 0 and 0.5 hours, between 0.5 and 1.5 hours, between 1.5 and 3 hours, between 3 and 9 hours, between 9 and 24 hours, or more than 24 hours, and / or the time between centrifugation of the sample and decanting of the sample is between 0 and 0.5 hours, between 0.5 and 1.5 hours, between 1.5 and 3 hours, between 3 and 9 hours, between 9 and 24 hours, or more than 24 hours.

9. 1. A method for measuring protein levels to assess sample handling and / or sample processing, comprising: a) contacting a sample from a human subject with a series of capture reagents, each capture reagent having an affinity for a different protein from a series of proteins including IHH and at least one protein selected from the group consisting of PGAM1, PTPN4, TNFSF14, FAM49B, RBP7, SHH, DDX39B, S100A12, ADAM9, PGAM2, C4A.C4B, IL21R, and TMEM9; b) determining a level of each of said set of proteins based on said set of capture reagents, wherein said sample is selected from blood, plasma, or serum; The method includes:

10. The method of claim 9 , wherein the set of capture reagents is selected from aptamers, antibodies, and combinations of aptamers and antibodies.

11. The method of claim 9 or 10, comprising measuring IHH and PGAM2, IHH and C4A / C4B, IHH and PTPN4, IHH and TNFSF14, IHH and PGAM1, IHH and FAM49B, IHH and RBP7, IHH and DDX39B, IHH and S100A12, IHH and IL21R, IHH and TMEM9, or IHH and ADAM9.

12. IHH, PGAM1, and PGAM2; IHH, PGAM1, and C4A and C4B; HH, PGAM1, and PTPN4; IHH, PGAM1, and TNSF14; IHH, PGAM1, and FAM49B; IHH, PGAM1, and RBP7; IHH, PGAM1, and DDX39B; IHH, PGAM1, and S100A12; IHH, PGAM1, and IL21R; IHH, PGAM1, and TMEM9; IHH, PGAM1, and ADAM9; IHH, PGAM2, and C4A-C4B; IHH, PGAM2, and PTPN4; IHH, PGAM2, and TNSF14; IHH, PGAM2, and FAM49B; IHH, PGAM 2, and RBP7; IHH, PGAM2, and DDX39B; IHH, PGAM2, and S100A12; IHH, PGAM2, and IL21R; IHH, PGAM2, and TMEM9; IHH, PGAM2, and ADAM9; IHH, C4A·C4B, and PTPN4; IHH, C4A·C4B, and TNSF14; IHH, C4A·C4B, and FAM49B; IHH, C4A·C4B, and RBP7; IHH, C4A·C4B, and DDX39B; IHH, C4A·C4B, and S100A12; IHH, C4A·C4B, and IL21R; IHH, C4A / C4B, and TMEM9; IHH, C4A / C4B, and ADAM9; IHH, PTPN4, and TNFSF14; IHH, PTPN4, and FAM49B; IHH, PTPN4, and RBP7; IHH, PTPN4, and DDX39B; IHH, PTPN4, and S100A12; IHH, PTPN4, and IL21R; IHH, PTPN4, and TMEM9; IHH, PTPN4, and ADAM9; IHH, TNFSF14, and FAM49B; IHH, TNFSF14, and RBP7; IHH, TNFSF14, and DDX39B; IHH , TNFSF14, and S100A12; IHH, TNFSF14, and IL21R; IHH, TNFSF14, and TMEM9; IHH, TNFSF14, and ADAM9; IHH, FAM49B, and RBP7; IHH, FAM49B, and DDX39B; IHH, FAM49B, and SA100A12; IHH, FAM49B, and IL21R; IHH, FAM49B, and TMEM9; IHH, FAM49B, and ADAM9; IHH, RBP7, and DDX39B; IHH, RBP7, and S100A12; IHH, RBP7, and IL21R;11. The method of claim 9 or 10, comprising measuring IHH, RBP7, and TMEM9; IHH, RBP7, and ADAM9; IHH, DDX39B, and S100A12; IHH, DDX39B, and IL21R; IHH, DDX39B, and TMEM9; IHH, DDX39B, and ADAM9; IHH, S100A12, and IL21R; IHH, S100A12, and TMEM9; IHH, S100A12, and ADAM9; IHH, IL21R, and TMEM9; IHH, IL21R, and ADAM9; or IHH, TMEM9, and ADAM9.

13. The method of claim 9 or 10, comprising measuring at least two proteins selected from IHH and PGAM1, and RBP7, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, SHH, PGAM2, C4A / C4B, IL21R, TMEM9, and ADAM9.

14. The method according to claim 9 or 10, comprising measuring at least two proteins selected from IHH and RBP7, and SHH, TNFSF14, PTPN4, DDX39B, FAM49B, S100A12, PGAM1, PGAM2, C4A / C4B, IL21R, TMEM9 and ADAM9.

15. 15. The method of any one of claims 9 to 14, wherein the level of the protein is used to predict the length of time between taking a sample from the human subject and centrifugation of the sample, and / or the length of time between centrifugation of the sample and decanting of the sample.

16. 16. The method of claim 15, wherein the time between taking the sample and centrifugation of the sample is between 0 and 0.5 hours, between 0.5 and 1.5 hours, between 1.5 and 3 hours, between 3 and 9 hours, between 9 and 24 hours, or more than 24 hours; and / or the time between centrifugation of the sample and decanting of the sample is between 0 and 0.5 hours, between 0.5 and 1.5 hours, between 1.5 and 3 hours, between 3 and 9 hours, between 9 and 24 hours, or more than 24 hours.

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