Animal nutritional composition

Abstract

To provide a novel nutrient composition for animals.SOLUTION: The present invention discloses a nutrient composition for a race horse, a nutrient composition for animals that suppresses a rise in the blood lactic acid concentration, and a nutrient composition for animals that improves coat, where these compositions contain nicotinamide mononucleotide.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to nutritional compositions for animals. [Background technology]

[0002] Nicotinamide mononucleotide is the coenzyme NAD + It is a biosynthetic intermediate metabolite of . In Non-Patent Document 1, it is reported that in a clinical trial of humans by oral ingestion, nicotinamide mononucleotide has the effects of improving obesity, preventing diabetes, and improving muscle insulin sensitivity. In addition, in Non-Patent Document 2, it is reported that in a test using mice by oral ingestion, nicotinamide mononucleotide has the effects of suppressing weight gain, improving insulin sensitivity, improving lipid metabolism, improving visual ability, increasing bone density, and increasing immune function. On the other hand, there have been no known cases of orally ingesting nicotinamide mononucleotide to racehorses. [Prior art documents] [Non-patent literature]

[0003] [Non-Patent Document 1] Mihoko Yoshino, Jun Yoshino, Brandon D Kayser, Gary J Patti, Michael P Franczyk, Kathryn F Mills, Miriam Sindelar, Terri Pietka, Bruce W Patterson, Shin-Ichiro Imai, Samuel Klein, Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women, Science 2021, 372, 1224-1229. [Non-Patent Document 2] Kathryn F. Mills, Shohei Yoshida, Liana R. Stein, Koji Uchida, Jun Yoshino, Shin-ichiro Imai, Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice Cell Metabolism 2016, 24, 795-806. Summary of the Invention [Problem to be solved by the invention]

[0004] An object of the present invention is to provide a novel nutritional composition for animals. [Means for solving the problem]

[0005] The present invention includes the following. [1] A nutritional composition for racehorses containing nicotinamide mononucleotide. [2] An animal nutritional composition for suppressing an increase in blood lactate concentration, comprising nicotinamide mononucleotide. [3] An animal nutritional composition for improving coat condition, comprising nicotinamide mononucleotide. [4] The animal nutritional composition according to [2] or [3], which is used for racehorses. Effect of the Invention

[0006] The animal nutritional composition of the present invention can suppress an increase in blood lactate concentration and / or improve the coat condition of racehorses. [Brief description of the drawings]

[0007] [Figure 1] FIG. 1 is a graph showing the measurement results of lactate concentration in Test Example 1. [Diagram 2] FIG. 1 is a graph showing average values ​​of lactate concentration in Test Example 1. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] [Nutritional composition] The nutritional composition of the present invention (hereinafter also referred to as "the present nutritional composition") contains nicotinamide mononucleotide (hereinafter also referred to as "NMN").

[0009] <nmn> NMN(Chemical formula:C 11 H 15 N2O8P) has two optical isomers, α and β, but in the present invention, β-NMN (CAS number: 1094-61-7) is used. The structure of β-NMN is shown below.

[0010] [ka]

[0011] β-NMN may be prepared by any method. For example, β-NMN that has been artificially synthesized by chemical synthesis, enzymatic methods, fermentation, or other methods and then purified can be used. In addition, since β-NMN is a component that is widely present in living organisms, β-NMN obtained by extraction and purification from natural raw materials such as animals, plants, and microorganisms can also be used. Commercially available purified β-NMN may also be used.

[0012] As a chemical synthesis method for synthesizing β-NMN, for example, nicotinamide and L-ribose tetraacetate are reacted, and the resulting nicotinamide mononucleoside is phosphorylated to produce β-NMN. As an enzymatic method, for example, β-NMN can be produced from nicotinamide and 5'-phosphoribosyl-1'-pyrophosphate (PRPP) using nicotinamide phosphoribosyltransferase (NAMPT). As a fermentation method, for example, β-NMN can be produced from nicotinamide using the metabolic system of a microorganism expressing NAMPT.

[0013] The NMN contained in the nutritional composition may be a pharmacologically acceptable salt of β-NMN. The pharmacologically acceptable salt of β-NMN may be an inorganic acid salt or an organic acid salt having a basic site such as an amine. Examples of acids constituting such acid salts include acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethenesulfonic acid, fumaric acid, gluconic acid, glutamic acid, hydrobromic acid, hydrochloric acid, isethionic acid, lactic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, mucic acid, nitric acid, pamoic acid, pantothenic acid, phosphoric acid, succinic acid, sulfuric acid, tartaric acid, p-toluenesulfonic acid, etc. In addition, the pharmacologically acceptable salt of β-NMN may be an alkali salt or an organic salt having an acidic site such as a carboxylic acid. Examples of bases that constitute such acid salts include alkali metal salts or alkaline earth metal salts, such as those derived from bases such as sodium hydride, potassium hydroxide, calcium hydroxide, aluminum hydroxide, lithium hydroxide, magnesium hydroxide, zinc hydroxide, ammonia, trimethylammonia, triethylammonia, ethylenediamine, lysine, arginine, ornithine, choline, N,N'-dibenzylethylenediamine, chloroprocaine, procaine, diethanolamine, N-benzylphenethylamine, diethylamine, piperazine, tris(hydroxymethyl)-aminomethane, and tetramethylammonium hydroxide.

[0014] The NMN contained in the nutritional composition may be free β-NMN or a solvate of a pharmacologically acceptable salt of β-NMN. Solvents that form the solvate include water, ethanol, etc.

[0015] The content of NMN contained in the present nutritional composition is not limited, but for example, when the total amount of the present nutritional composition is 100 parts by mass, it may be 100 parts by mass, it may be 1 to 99.9 parts by mass, or it may be 1 to 50 parts by mass.

[0016] <Other ingredients> The nutritional composition may contain components other than NMN. The nutritional composition may contain, for example, various vitamins (vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, etc.), various minerals, dietary fiber, polyunsaturated fatty acids, other nutrients (carotenoids such as β-carotene, lycopene, astaxanthin, carnitine, sesamin), stabilizers such as dispersants and emulsifiers, sweeteners, taste components (citric acid, malic acid, etc.), flavors, royal jelly, propolis, agaricus, etc. Herbs such as peppermint, bergamot, chamomile, and lavender may also be included.

[0017] <Dosage form> Examples of the form of the animal nutritional composition of the present invention include powders, powders, granules, soft capsules, hard capsules, tablets, chewable tablets, rapidly disintegrating tablets, syrups, liquids, suspensions, suppositories, ointments, creams, gels, adhesives, inhalants, and injections.

[0018] Examples of additives that can be used for formulation include animal oils such as soybean oil, saffron oil, olive oil, germ oil, sunflower oil, beef tallow, and sardine oil, polyhydric alcohols such as polyethylene glycol, propylene glycol, glycerin, and sorbitol, surfactants such as sorbitan fatty acid esters, sucrose fatty acid esters, glycerin fatty acid esters, and polyglycerin fatty acid esters, excipients such as purified water, lactose, starch, crystalline cellulose, D-mannitol, lecithin, gum arabic, sorbitol liquid, and sugar liquid, sweeteners, colorants, pH adjusters, and flavors. Liquid formulations may be in a form that is dissolved or suspended in water or other suitable medium when taken. Tablets and granules may be coated by a well-known method.

[0019] <Application> The nutritional composition can be ingested by animals as it is or as an animal feed composition mixed with a basic animal feed. The nutritional composition can be ingested by animals throughout the entire rearing period or during a specific period of the rearing period.

[0020] A suitable example of an animal to which the nutritional composition is administered is a racehorse. By administering the composition to a racehorse, the increase in the lactic acid concentration in the blood can be suppressed, so that the horse is less likely to suffer from muscle fatigue and muscle pain, which can contribute to improving the results of the racehorse. Racehorses include horses aged 2 to 7 years, and also include young horses being kept. The dosage varies depending on the horse's years of breeding, weight, sex, etc., but each administration contains, for example, 1 g to 10 g of NMN. The daily dosage of NMN is, for example, 1 g to 30 g, preferably 1 g to 20 g, and more preferably 1 g to 15 g.

[0021] Animals other than racehorses to which the nutritional composition may be administered include animals other than humans, such as birds (e.g., chickens, quail, turkeys, ducks, etc.; e.g., poultry), mammals (bovine animals (e.g., domestic cattle (e.g., dairy cattle and / or beef cattle), bison, buffalo), equine animals (e.g., horses, donkeys, zebras, etc.), sheep, goats, antelope, pigs, etc.). Examples of livestock include poultry, such as chickens (broilers, layers, etc.).

[0022] The nutritional composition can be used to suppress an increase in the blood lactate concentration of an animal to which it is administered, or to improve the condition of the animal's coat. Improving the condition of the coat means improving the luster, quality, condition of growth, color, etc. of the body hair.

[0023] [Animal feed composition] The animal feed composition of the present invention (hereinafter also referred to as the "animal feed composition") contains NMN and a basal feed. The animal feed composition may be in the form of the present nutritional composition and a basal feed. The details of the components contained in the nutritional composition are as described above.

[0024] The basal feed may be a commercially available basal feed, such as oil cakes, animal feed ingredients, grains and their processed products, animal fats and oils, vegetable fats and oils, or a mixture of these. Oil cakes include dried foods such as corn, milo, and sorghum, soybean fats and oils, rapeseed fats and oils, sesame fats and oils, cottonseed fats and oils, etc. Animal feed ingredients include fish meal, meat and bone meal, etc. Grains include starch, soybeans, wheat, oats, etc. Animal fats and oils include meat meal, chicken meal, lard, beef tallow, etc. Vegetable fats and oils include rapeseed oil, palm oil, coconut oil, soybean oil, safflower oil, corn oil, cottonseed oil, etc. The CP in basal feed stands for crude protein (one of the six components of feed, 6.25 times the nitrogen content), and basal feeds such as CP 18% (crude protein content of 18%) and CP 19% can be adopted.

[0025] The basal feed may contain additives other than the nutritional composition. Here, the additives may be, for example, seaweed. Seaweed contains a lot of nutrients such as minerals such as calcium, zinc, and iodine, dietary fiber such as sodium alginate, potassium alginate, and calcium alginate, polysaccharides such as fucoidan and funoran, and aminopeptides such as nori peptides and wakame peptides. Another additive may be collagen, which may be in the form of, for example, an extract, a dried product, a crushed product, or a powder.

[0026] In addition to the above, examples of additives include fatty acids such as linoleic acid, linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid; minerals such as salt, calcium carbonate, calcium phosphate, and anhydrous silicic acid; vitamins such as vitamin A, vitamin B2, vitamin D, vitamin E, vitamin K, biotin, folic acid, and pantothenic acid; amino acids such as lysine, glycine, and methionine; emulsifiers such as sorbitan fatty acid esters, sucrose fatty acid esters, and lecithin; live bacteria agents such as lactic acid bacteria; trace minerals such as iron, zinc, manganese, and copper; mushrooms or mushroom components such as spaghetti mushroom, maitake mushroom, shiitake mushroom, and cordyceps sinensis; sake lees, antioxidants, antifungal agents, and dietary fiber.

[0027] The content of NMN in the animal feed composition is not particularly limited, but when the basal feed is taken as 100 parts by mass, it can be, for example, 0.005 to 20 parts by mass, preferably 0.01 to 10 parts by mass, and more preferably 0.02 to 5 parts by mass. The dosage of the animal feed composition varies depending on the age, weight, sex, etc. of the animal, but for example, 1 kg to 8 kg is administered at one time, and for example, 18 kg to 24 kg can be given per day.

[0028] The details of the target animals to be fed with the animal feed composition are as described above. EXAMPLES

[0029] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0030] [Preparation of nutritional composition] Nutritional composition A having the composition shown in Table 1 and basal feed A (containing oats, soybean meal (manufactured by Nosan Corporation), and feed (product name: Wizard, manufactured by Crossfield Co., Ltd.)) were prepared.

[0031] [Table 1]

[0032] [Test Example 1] In Test Example 1, four racehorses (Racehorse A (7 years old), Racehorse B (4 years old), Racehorse C (8 years old), and Racehorse D (3 years old)) were fed as described below, and their blood lactate concentrations and coat condition were evaluated.

[0033] <Evaluation of blood lactate concentration> Each racehorse was administered basal feed A for 14 consecutive days. The amount of feed was 6kg-8kg per session, three times a day. On the 14th day, after 80-100 minutes of regular training (60 minutes on a walking machine, 20-40 minutes of training), blood was collected from the vein in the neck and the blood lactate concentration was measured using a lactate measuring device (product name: Lactate Pro 2LT-1730, manufactured by Arkray, Inc.) (hereinafter also referred to as "pre-intake test"). After that, each racehorse was fed basal feed A and nutritional composition A for 14 consecutive days. The amount of feed was 6kg-8kg of basal feed A per session, 12g of nutritional composition A per session, three times a day, so that the daily amount of NMN was 5.0-5.5g. After 80 to 100 minutes of regular training, the blood lactate concentration was measured by the same method as above (hereinafter, also referred to as "post-intake test"). Figure 1 shows the measurement results of the lactate concentration of four horses, and Figure 2 shows the average lactate concentration of the four horses.

[0034] <Evaluation of coat condition> The coat condition of each racehorse was visually compared before and after the intake test, and the coat condition after the intake test was evaluated according to the following criteria. The evaluation results are shown in Table 2. 3: Good improvement in gloss and texture was observed. 2: Slight improvement in gloss and texture was observed. 1: No improvement in coat condition was observed.

[0035] [Table 2]

[0036] From the results shown in Figures 1 and 2, it was found that nutritional composition A has the effect of suppressing the increase in the blood lactate concentration of racehorses. In addition, from the results shown in Table 2, it was found that nutritional composition A has the effect of improving the coat condition of racehorses.< / nmn>

Claims

1. An animal nutritional composition for improving coat condition, for use in equine animals, comprising nicotinamide mononucleotide.

2. 1. A method for improving the appearance of an equine coat, comprising: Feeding a nutritional composition containing nicotinamide mononucleotide together with a basal feed; In the above step, the nicotinamide mononucleotide is fed to the rat at a rate of 1 g to 30 g per day.

3. The method according to claim 2, wherein in the step, the nicotinamide mononucleotide is fed at 5.0 g to 5.5 g per animal per day.

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