Compositions and methods comprising prostate stem cell antigen (PSCA) chimeric antigen receptor (CAR)

Abstract

The present disclosure provides modified immune cells or their precursors (e.g., T cells) comprising a chimeric antigen receptor (CAR) capable of binding to human PSCA. CARs capable of binding to human PSCA and nucleic acids encoding the CARs are also provided. Provided herein are bispecific CARs capable of binding to human PSCA and human PSMA, nucleic acids encoding the bispecific CARs, and modified immune cells comprising the bispecific CARs. Modified immune cells comprising PSMA CARs and PSCA CARs are also provided. Compositions and methods of treatment are also provided. TIFF2022547552000032.tif137169

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application No. 62 / 985,808, filed March 5, 2020, and U.S. Provisional Patent Application No. 62 / 898,896, filed September 11, 2019, each of which is incorporated by reference in its entirety. [Background technology]

[0002] 2. Background of the Invention Prostate cancer is the most common cancer diagnosis and the second leading cause of cancer-related deaths in men in the United States. Despite recent advances in the detection and treatment of localized disease, there remain significant challenges in managing the disease. Current diagnostic methods are limited by a lack of specificity and an inability to predict which patients are at risk for developing metastatic disease. Prostate-specific antigen (PSA) is effective in identifying men who may have prostate cancer, but is often elevated in men with benign prostatic hyperplasia, prostatitis, and other non-malignant disorders. PSA and other current markers cannot accurately distinguish between indolent and aggressive cancers. For the 20-40% of patients whose disease recurs after surgery or radiation therapy or who have metastatic disease at the time of diagnosis, there is no effective treatment. Hormone deprivation therapy can provide temporary relief for these patients, but the majority will inevitably progress and develop incurable androgen-independent disease.

[0003] There is a need in the art for compositions and methods for treating prostate cancer. The present invention addresses this need. Summary of the Invention

[0004] The present invention is based on the finding that prostate stem cell antigen (PSCA) chimeric antigen receptor (CAR) T cells show strong antitumor activity. The present invention is also based on the finding that bispecific CARs and dual CARs capable of binding to PSCA and prostate specific membrane antigen (PSMA) show significantly enhanced antitumor activity.

[0005] Thus, in certain aspects, the present disclosure provides a chimeric antigen receptor (CAR) comprising an antigen binding domain capable of binding to prostate stem cell antigen (PSCA), a transmembrane domain, and an intracellular domain.

[0006] In certain exemplary aspects, the antigen binding domain comprises a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6).

[0007] In certain exemplary embodiments, the heavy chain variable region comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, and / or the light chain variable region comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8.

[0008] In certain exemplary embodiments, the antigen-binding domain is a single-chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9.

[0009] In certain exemplary embodiments, the intracellular domain comprises a costimulatory domain of a protein selected from the group consisting of proteins in the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, and B7-H3 (CD276), or variants thereof, or an intracellular domain derived from a killer immunoglobulin-like receptor (KIR).

[0010] In certain exemplary embodiments, the CAR is capable of binding to prostate stem cell antigen (PSCA) and comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:21, 23, 25 or 27.

[0011] In another aspect, the present disclosure provides a bispecific chimeric antigen receptor (CAR), comprising an extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen-binding domain capable of binding to prostate specific membrane antigen (PSMA), a transmembrane domain, and an intracellular domain.

[0012] In certain exemplary aspects, the extracellular domain comprises an antigen binding domain capable of binding to PSCA comprising: a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The extracellular domain also comprises an antigen-binding domain capable of binding to PSMA, comprising: a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0013] In certain exemplary embodiments, the first heavy chain variable region of the bispecific CAR comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; the first light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or the second heavy chain variable region of the bispecific CAR comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34; the second light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:35. It comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:35.

[0014] In certain exemplary embodiments, the antigen binding domain capable of binding to PSCA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9.

[0015] In another aspect, the present disclosure provides a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:40, 42, 80, 81 or 82.

[0016] In another aspect, the disclosure provides a nucleic acid comprising a polynucleotide sequence encoding any of the CARs or bispecific CARs disclosed herein.

[0017] In another aspect, the present disclosure provides a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an antigen binding domain, a transmembrane domain, and an intracellular domain. The antigen binding domain comprises a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), HCDR1 comprising the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprising the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprising the amino acid sequence TGGF (SEQ ID NO:3); and a light chain variable region comprising three light chain complementarity determining regions (LCDRs), LCDR1 comprising the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprising the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprising the amino acid sequence QQWSSSPFT (SEQ ID NO:6).

[0018] In certain exemplary embodiments, the antigen binding domain comprises a heavy chain variable region encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43; and / or a light chain variable region encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44; and / or the antigen binding domain is a single chain variable fragment (scFv) encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:45.

[0019] In another aspect, the disclosure provides a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:20, 22, 24 or 26.

[0020] In another aspect, the present disclosure provides a nucleic acid comprising a polynucleotide sequence encoding a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), wherein the bispecific CAR comprises an extracellular domain comprising an antigen binding domain capable of PSCA and an antigen binding domain capable of binding to PSMA, a transmembrane domain, and an intracellular domain.

[0021] In certain exemplary aspects, the extracellular domain comprises an antigen binding domain capable of binding to PSCA comprising: a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The extracellular domain also comprises an antigen-binding domain capable of binding to PSMA, comprising: a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0022] In certain exemplary embodiments, the first heavy chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43; the first light chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44; and / or the second heavy chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:46; and / or the second light chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:47. and / or the nucleic acid comprises a costimulatory domain of a protein selected from the group consisting of a protein within the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, ICOS, and B7-H3 (CD276), or a variant thereof, or an intracellular domain comprising an intracellular domain derived from a killer immunoglobulin-like receptor (KIR).

[0023] In certain exemplary embodiments, the bispecific CAR is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:39 or 41 or 79.

[0024] In another aspect, the disclosure provides a vector comprising any of the nucleic acids contemplated herein.

[0025] In certain exemplary embodiments, the vector is an expression vector; and / or the vector is selected from the group consisting of a DNA vector, an RNA vector, a plasmid, a lentiviral vector, an adenoviral vector, an adeno-associated viral vector, and a retroviral vector.

[0026] In another aspect, the present disclosure provides an engineered immune cell or a precursor thereof comprising any of the CARs contemplated herein, any of the nucleic acids contemplated herein, or any of the vectors contemplated herein.

[0027] In another aspect, the present disclosure provides an engineered immune cell, or a precursor thereof, comprising a chimeric antigen receptor (CAR) comprising an antigen binding domain capable of binding to prostate stem cell antigen (PSCA), a transmembrane domain, and an intracellular domain.

[0028] In certain exemplary embodiments, the modified cells further comprise a PSMA-CAR, which comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain.

[0029] In another aspect, the disclosure provides an engineered immune cell, or a precursor thereof, comprising a first chimeric antigen receptor (CAR) comprising a first antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and a second CAR comprising a second antigen binding domain capable of binding to prostate specific membrane antigen (PSMA), wherein the first and second CAR each comprise a transmembrane domain and an intracellular domain.

[0030] In certain exemplary aspects, the modified cells further comprise a switch receptor.

[0031] In certain exemplary embodiments, at least one of the CARs is capable of binding to human PSCA; and / or at least one of the CARs is capable of binding to human PSMA; and / or the modified cell is an modified immune cell; and / or the modified cell is an modified immune cell that is an modified T cell.

[0032] In another aspect, the disclosure provides a pharmaceutical composition comprising a therapeutically effective amount of any of the modified cells contemplated herein.

[0033] In another aspect, the disclosure provides a method of treating a disease in a subject in need thereof comprising administering to the subject an effective amount of any of the modified cells contemplated herein, or any of the pharmaceutical compositions contemplated herein.

[0034] In certain exemplary embodiments, the disease is cancer; and / or the disease is cancer and the cancer is prostate cancer; and / or the disease is cancer and the cancer is metastatic castration-resistant prostate cancer.

[0035] In another aspect, the present disclosure provides a method of treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of modified T cells comprising a first chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second chimeric antigen receptor (CAR) capable of binding to a prostate specific membrane antigen (PSMA-CAR), wherein the first CAR and the second CAR each comprise an antigen binding domain, a transmembrane domain, and an intracellular domain.

[0036] In another aspect, the present disclosure provides a method for treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of modified T cells comprising a bispecific chimeric antigen receptor (CAR). The bispecific CAR comprises an extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen-binding domain capable of binding to prostate specific membrane antigen (PSMA), a transmembrane domain, and an intracellular domain.

[0037] In certain exemplary embodiments, the engineered T cells further comprise a dominant negative receptor; and / or the engineered T cells further comprise a switch receptor.

[0038] In certain exemplary aspects, the method further comprises administering lymphodepleting chemotherapy to the subject.

[0039] In certain exemplary aspects, the subject is a human.

[0040] [The present invention 1001] A chimeric antigen receptor (CAR) comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA), a transmembrane domain, and an intracellular domain. [The present invention 1002] The antigen-binding domain is (a) a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and (b) a light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The CAR of the present invention 1001, comprising: [The present invention 1003] (a) the heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and / or (b) the light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; The CAR of the present invention 1002. [The present invention 1004] A CAR of any of claims 1001 to 1003, wherein the antigen-binding domain is a single-chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9. [The present invention 1005] The CAR of any of the present inventions 1001 to 1004, wherein the intracellular domain comprises a costimulatory domain of a protein selected from the group consisting of proteins in the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, and B7-H3 (CD276), or variants thereof, or an intracellular domain derived from a killer immunoglobulin-like receptor (KIR). [The present invention 1006] A chimeric antigen receptor (CAR) of any of claims 1001 to 1005, which is capable of binding to prostate stem cell antigen (PSCA) and comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 21, 23, 25 or 27. [The present invention 1007] (a) an extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA); (b) an antigen-binding domain capable of binding to prostate-specific membrane antigen (PSMA); (c) a transmembrane domain; and (d) Intracellular domain A bispecific chimeric antigen receptor (CAR). [The present invention 1008] (a) an antigen-binding domain capable of binding to PSCA, (i) a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); (ii) a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6); and Includes; (b) an antigen-binding domain capable of binding to PSMA, (i) a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); (ii) a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33); and Including, The bispecific CAR of the present invention 1007. [The present invention 1009] (a) the first heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; the first light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or (b) the second heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34; and the second light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:35. The bispecific CAR of the present invention 1008. [The present invention 1010] Any of the bispecific CARs of the present invention 1007 to 1009, wherein the antigen-binding domain capable of binding to PSCA is a single-chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9. [The present invention 1011] Any of the bispecific CARs of the present invention 1007 to 1010, which is capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA) and comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 40, 42, 80, 81 or 82. [The present invention 1012] A nucleic acid comprising a polynucleotide sequence encoding any one of the CARs of the present invention 1001 to 1006. [The present invention 1013] A nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), the CAR comprising an antigen binding domain, a transmembrane domain, and an intracellular domain, The antigen-binding domain is (a) a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); (b) a light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6); A nucleic acid comprising: [The present invention 1014] (a) the antigen-binding domain comprises a heavy chain variable region encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43; and / or (b) the antigen-binding domain comprises a light chain variable region encoded by a polynucleotide sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44; and / or (c) the antigen-binding domain is a single-chain variable fragment (scFv) encoded by a polynucleotide sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:45; The nucleic acid of the present invention 1013. [The present invention 1015] Any of the nucleic acids of 1013 to 1014 of the present invention, comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), the polynucleotide sequence being at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 20, 22, 24 or 26. [The present invention 1016] A nucleic acid comprising a polynucleotide sequence encoding any one of the bispecific CARs of the present invention 1007 to 1011. [The present invention 1017] A nucleic acid comprising a polynucleotide sequence encoding a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), wherein the bispecific CAR comprises an extracellular domain comprising an antigen binding domain capable of PSCA and an antigen binding domain capable of binding to PSMA, a transmembrane domain, and an intracellular domain. [The present invention 1018] (a) an antigen-binding domain capable of binding to PSCA, (i) a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); (ii) a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6); and Includes; (b) an antigen-binding domain capable of binding to PSMA, (i) a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); (ii) a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33); and Including, The nucleic acid of the present invention 1017. [The present invention 1019] (a) a first heavy chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43; and a first light chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44; and / or (b) the second heavy chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:46; and the second light chain variable region is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:47; and / or (c) the nucleic acid comprises an intracellular domain, including a costimulatory domain of a protein selected from the group consisting of a protein in the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, ICOS, and B7-H3 (CD276), or a variant thereof, or an intracellular domain comprising an intracellular domain derived from a killer immunoglobulin-like receptor (KIR); The nucleic acid of the present invention 1017 or 1018. [The present invention 1020] Any of the nucleic acids of 1017 to 1019, wherein the bispecific CAR is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 39 or 41 or 79. [The present invention 1021] A vector comprising any one of the nucleic acids of the present invention 1012 to 1020. [The present invention 1022] (a) is an expression vector; and / or (b) selected from the group consisting of a DNA vector, an RNA vector, a plasmid, a lentiviral vector, an adenoviral vector, an adeno-associated viral vector, and a retroviral vector; The vector of the present invention. [The present invention 1023] A modified immune cell or a precursor thereof comprising a CAR of any one of claims 1001 to 1011, a nucleic acid of any one of claims 1012 to 1020, or a vector of any one of claims 1021 to 1022. [The present invention 1024] An engineered immune cell, or a precursor thereof, comprising a chimeric antigen receptor (CAR) comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA), a transmembrane domain, and an intracellular domain. [The present invention 1025] The modified cell of the present invention 1024, further comprising a PSMA-CAR, wherein the PSMA-CAR comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain. [The present invention 1026] (a) a first chimeric antigen receptor (CAR) comprising a first antigen-binding domain capable of binding to prostate stem cell antigen (PSCA); (b) a second CAR comprising a second antigen-binding domain capable of binding to prostate-specific membrane antigen (PSMA); and A modified immune cell or a precursor thereof, comprising: An engineered immune cell or a precursor thereof, wherein the first and second CARs each comprise a transmembrane domain and an intracellular domain. [The present invention 1027] The modified cell of any of claims 1023 to 1026, further comprising a switch receptor. [The present invention 1028] (a) at least one of the CARs is capable of binding to human PSCA; and / or (b) at least one of the CARs is capable of binding to human PSMA; and / or (c) the modified cell is a modified immune cell; and / or (d) the modified cell is a modified immune cell that is a modified T cell; The modified cell of any one of claims 1023 to 1027. [The present invention 1029] A pharmaceutical composition comprising a therapeutically effective amount of the modified cells of any one of the present inventions 1023 to 1028. [The present invention 1030] A method for treating a disease in a subject in need thereof, comprising administering to the subject an effective amount of the modified cell of any of the present inventions 1023 to 1028, or the pharmaceutical composition of the present invention 1029. [The present invention 1031] (a) the disease is cancer; and / or (b) the disease is cancer, and the cancer is prostate cancer; and / or (c) the disease is cancer, and the cancer is metastatic castration-resistant prostate cancer; The method of the present invention 1030. [The present invention 1032] (a) a first chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA-CAR); (b) a second chimeric antigen receptor (CAR) capable of binding to prostate-specific membrane antigen (PSMA-CAR); administering to a subject an effective amount of modified T cells comprising 1. A method of treating prostate cancer in a subject in need thereof, comprising: The method, wherein the first CAR and the second CAR each comprise an antigen-binding domain, a transmembrane domain, and an intracellular domain. [The present invention 1033] 1. A method of treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of an engineered T cell comprising a bispecific chimeric antigen receptor (CAR) comprising an extracellular domain, a transmembrane domain, and an intracellular domain, the extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen-binding domain capable of binding to prostate specific membrane antigen (PSMA). [The present invention 1034] (a) the modified T cell further comprises a dominant negative receptor; and / or (b) the engineered T cell further comprises a switch receptor; Any of the methods according to claims 1030 to 1033 of the present invention. [The present invention 1035] The method of any one of claims 1030 to 1034, further comprising the step of administering lymphodepleting chemotherapy to the subject. [The present invention 1036] Any of the methods of 1030 to 1035, wherein the subject is a human. The foregoing and other features and advantages of the present invention will be more fully understood from the following detailed description of illustrative embodiments, taken in conjunction with the accompanying drawings. [Brief description of the drawings]

[0041] [Figure 1]Figure 1 illustrates the generation of a prostate stem cell antigen (PSCA)-specific CAR. A PSCA CAR composed of scFv derived from a humanized anti-PSCA Ab (2B3) was constructed and cloned into the retroviral vector MSGV. [Diagram 2] FIG. 2 illustrates CAR expression of T cells electroporated with in vitro transcribed RNA of a PSCA CAR with 4-1BB and CD3z domains (2B3.BBZ) and / or a PSMA CAR with 4-1BB and CD3z domains (J591.BBZ) (top panel), as well as PD-L1-Fc staining of PSCA CARs co-electroporated with bispecific antibodies 10A5-1412 (aPDL1-aCD28 bispecific Ab; designated as 10A5) or TGFB3-1412 (aTGFbRII-aCD28 bispecific Ab; designated as TGFB3). [Diagram 3] Figure 3 illustrates CD107a expression in RNA co-electroporated T cells stimulated with PC3-PSCA-PSMA or K562. T cells were electroporated with PSCA CAR (2B3.BBZ) with 4-1BB and CD3z domains and / or PSMA CAR (J591.BBZ) with 4-1BB and CD3z domains, as indicated. [Figure 4]Figure 4 illustrates that T cells lentivirally transduced with a PSCA(2B3) CAR with a mutant ICOS signaling domain (ICOS.YMNM) exhibited improved lytic capacity, reduced in vitro cytokine production, and in vivo antitumor activity equivalent to 4-1BB signaling PSCA CAR. PSCA CARs with either ICOS or ICOS.YMNM signaling domains were constructed and cloned into lentiviral vectors. CAR expression levels were equivalent to either 4-1BB or CD28 signaling domain CARs (top). CAR-T cells were stimulated with PSCA-positive cell lines PC3.PSCA.PSMA.CBG or PC3.PSCA.PSMA.CBG.PD-L1 and examined for cytokine (IL-2 and IFN-gamma) production (bottom panel). Human lung cancer cell line A549 and human breast cancer cell line MDA468 were also used. Abbreviations: PSCA(2B3)CAR with ICOS and CD3z domain (2B3.ICOSz or PSCA.2B3.ICOSz); PSCA(2B3)CAR with variant ICOS (YMNM) and CD3z domain (2B3.ICOSz.YMNM or PSCA.2B3.ICOSz.YMNM); PSCA(2B3)CAR with CD28 and CD3z domain (2B3.28z or PSCA.2B3.28z); PSCA(2B3)CAR with ICOS and CD3z domain co-expressed with PD1-CD28 switch receptor (PD1.282B3.ICOSz or PD1.28.PSCA2B3.ICOSz); PSCA(2B3) CAR with variant ICOS (YMNM) and CD3z domain co-expressed with PD1-CD28 switch receptor (PD1.282B3.ICOSz.YMNM or PD1.28.PSCA.2B3.ICOSz.YMNM); PSCA(2B3) CAR with 4-1BB and CD3z domain (2B3.BBz or PSCA.2B3.BBz); PSCA(2B3) CAR with 4-1BB and CD3z domain co-expressed with PD1-CD28 switch receptor (2B3.BBz) with 4-1BB and CD3z domain co-expressed with PD1-CD28 switch receptor (PD1.28.2B3.BBz or PD1.28.PSCA.2B3.BBz); and non-transduced (no TD or NTD). [Figure 5A] Figure 5A illustrates the results of a CD107a assay of T cells expressing PSCA CARs with either ICOS or ICOS.YMNM signaling domains. The abbreviations used in Figure 5A are the same as those in Figure 4. [Figure 5B] Figure 5B illustrates the results of a killing assay for T cells expressing PSCA CARs with either ICOS or ICOS.YMNM signaling domains. The abbreviations used in Figure 5B are the same as those in Figure 4. [Figure 6] Figure 6 illustrates the results of an experiment using a PC3-PSCA-CBG-PDL1 tumor model. For lentiviral transduced (LVV TD) T cells, 1e6 cells per mouse were injected iv (intravenously) on day 21 after tumor inoculation. Five mice were tested per group. The abbreviations used in Figure 7 are the same as those in Figure 4. [Figure 7] Figure 7 illustrates the mean radiance of tumors after subcutaneous injection of various PSCA CARs. The abbreviations used in Figure 7 are the same as those in Figure 4. [Figure 8] Figure 8 illustrates tumor size after subcutaneous injection of various PSCA CARs. The abbreviations used in Figure 8 are the same as those in Figure 4. [Figure 9A]Figure 9A is a schematic diagram of the bispecific CAR vector used in the study encoding a PSMA (with 2F5 scFv)-targeted CAR and a PSCA (with 2B3 scFv)-targeted CAR linked by a Gly4Ser element. Abbreviations used in Figures 9A-9D: non-transducing (NTD); PSMA (2F5) CAR with 4-1BB and CD3z domains (2F5-BBZ); PSCA (2B3) CAR with 4-1BB and CD3z domains (2B3-BBZ); PSMA (2F5) and PSCA (2B3) bispecific CAR with 4-1BB and CD3z domains (2F5-S-2B3-BBZ; Gly4Ser linker); PSMA with 4-1BB and CD3z domains (2F5-S-2B3-BBZ; Gly4Ser linker); A(2F5) and PSCA(2B3) bispecific CAR (2F5-S-2B3-BBZ; (Gly4Ser)4 linker); PSCA(2B3) and PSMA(2F5) bispecific CAR with 4-1BB and CD3z domains (2B3-S-2F5-BBZ; Gly4Ser linker); PSCA(2B3) and PSMA(2F5) bispecific CAR with 4-1BB and CD3z domains (2B3-L-2F5-BBZ; (Gly4Ser)4 linker). [Figure 9B] Figure 9B illustrates the surface expression of CAR on lentiviral-transduced CAR T cells at the end of the first expansion. [Figure 9C] Figure 9C illustrates the percentage of lentiviral-transduced CAR T cells expressing PSMA or PSCA-CAR, or PSMA-PSCA bispecific CAR, as measured by staining with human recombinant PSMA-Fc and PSCA-His proteins and flow cytometry. [Figure 9D] Figure 9D illustrates that CAR T cells containing the indicated CARs were co-cultured with targets for 4 hours and the percentage of CD107a expression was quantified on CD8 positive cells. [Figure 10A]Figure 10A illustrates the results of co-culturing CAR T cells with PC3-PSCA cells (effector:target ratio = 1:1). Supernatants were obtained 24 hours after co-culture and analyzed for cytokine production by ELISA. The abbreviations used in Figures 10A-10D are the same as those used in Figures 9A-9D. [Figure 10B] FIG. 10B illustrates the results of testing the cytolytic activity of T cells against PC3-PSCA cells for 8 hours at the indicated E:T ratios. [Figure 10C] Figure 10C illustrates the results of co-culturing CAR T cells with PC3-PSMA cells (effector:target ratio = 1:1). Supernatants were obtained 24 hours after co-culture and analyzed for cytokine production by ELISA. [Figure 10D] FIG. 10D illustrates the results of testing the cytolytic activity of T cells against PC3-PSMA cells for 8 hours at the indicated E:T ratios. [Figure 11A] Figures 11A-11B illustrate the finding that TGFbR-IL12R switch receptor can enhance T cell function. Figure 11A (top right panel) shows IFN-gamma production of TGFbR-IL12R transfected NK cells co-cultured with K562 with or without TGFb1 in culture. Figure 11A (bottom right panel) shows pSmad staining of TGFbR-IL12R switch receptor transfected T cells after stimulation with TGF beta. [Figure 11B] Figures 11A-11B illustrate the finding that TGFbR-IL12R switch receptors can enhance T cell function. Figure 11B shows cytokine production of NY-ESO-1 positive tumors stimulated with NY-ESO-1 TCR transduced T cells co-transfected with TGFbR-IL12R switch receptors. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0042] Detailed Description The present invention provides compositions and methods for modified immune cells or their precursors (e.g., modified T cells) that contain a chimeric antigen receptor (CAR) that can bind to prostate stem cell antigen (PSCA).In certain embodiments, the present invention provides compositions and methods for modified immune cells or their precursors that contain a bispecific CAR (e.g., PSCA&PSMA), a PSCA CAR with a dominant negative receptor (e.g., TGFbRDN), a PSCA CAR with a switch receptor (e.g., PD1 / CD28 or TGFbR / IL12R), and a PSCA CAR combined with a bispecific antibody (e.g., PD-L1 / CD28).The provided compositions and methods are useful for treating cancer (e.g., prostate cancer).

[0043] It is to be understood that the methods described in this disclosure are not limited to the particular methods and / or experimental conditions disclosed herein, as such methods and conditions may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.

[0044] Furthermore, the experiments described herein use conventional molecular cell biology and immunological techniques within the skill of those skilled in the art, unless otherwise indicated.Such techniques are well known to skilled workers and are fully described in the literature.See, for example, Ausubel, et al., ed., Current Protocols in Molecular Biology, John Wiley & Sons, Inc., NY, NY (1987-2008), including all supplements, Molecular Cloning: A Laboratory Manual (Fourth Edition) by MR Green and J. Sambrook, and Harlow et al., Antibodies: A Laboratory Manual, Chapter 14, Cold Spring Harbor Laboratory, Cold Spring Harbor (2013, 2nd edition).

[0045] A. Definition Unless otherwise defined, scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. In the event of any potential meaning uncertainty, the definitions provided herein take precedence over any dictionary or external definitions. Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. Unless otherwise stated, the use of "or" means "and / or." The use of the term "including" and other forms such as "includes" and "included" are non-limiting.

[0046] In general, the nomenclature used in connection with cell and tissue culture, molecular biology, immunology, microbiology, genetics, and protein and nucleic acid chemistry and hybridization described herein is well known and widely used in the art.The methods and techniques provided herein are generally performed according to conventional methods well known in the art, unless otherwise indicated, as described in various general and more specific references cited and discussed throughout this specification.Enzymatic reactions and purification techniques are performed according to manufacturer's specifications as generally accomplished in the art or as described herein.The nomenclature used in connection with analytical chemistry, synthetic organic chemistry, and medicinal chemistry described herein, and the test procedures and techniques thereof are well known and widely used in the art.Standard techniques are used for chemical synthesis, chemical analysis, pharmaceutical preparation, formulation, and delivery, and patient treatment.

[0047] In order that this disclosure may be more readily understood, selected terms are defined below.

[0048] The articles "a" and "an" are used herein to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element.

[0049] As used herein, "about" when referring to a measurable value such as an amount, a temporal duration, and the like, is intended to encompass variations of ±20% or ±10%, more preferably ±5%, even more preferably ±1%, and even more preferably ±0.1% from the specified value, as such variations are reasonable in practicing the disclosed methods.

[0050] As used herein, "activation" refers to the state of T cells that are sufficiently stimulated to induce detectable cell proliferation.Activation can also be associated with induced cytokine production and detectable effector function.The term "activated T cells" refers specifically to T cells that undergo cell division.

[0051] As used herein, "alleviating" a disease means reducing the severity of one or more symptoms of the disease.

[0052] The term "antigen" as used herein is defined as a molecule that provokes an immune response. This immune response may include either or both antibody production or activation of specific immunologically competent cells. Those skilled in the art will appreciate that virtually any macromolecule can act as an antigen, including any protein or peptide.

[0053] Furthermore, the antigen can be derived from recombinant or genomic DNA. Those skilled in the art will understand that any DNA containing a nucleotide sequence or partial nucleotide sequence that encodes a protein that elicits an immune response therefore encodes an "antigen" as that term is used herein. Furthermore, those skilled in the art will understand that an antigen need not be encoded only by the full-length nucleotide sequence of a gene. It is readily apparent that the present invention includes, but is not limited to, the use of partial nucleotide sequences of more than one gene, and that these nucleotide sequences are arranged in various combinations to elicit a desired immune response. Furthermore, those skilled in the art will understand that an antigen need not be encoded by a "gene" at all. It is readily apparent that an antigen can be generated, synthesized, or derived from a biological sample. Such biological samples can include, but are not limited to, tissue samples, tumor samples, cells, or biological fluids.

[0054] As used herein, the term "autologous" is intended to refer to any material derived from the same individual that is subsequently reintroduced into that individual.

[0055] "Costimulatory molecule" refers to the cognate binding partner on a T cell that specifically binds to a costimulatory ligand, thereby mediating a costimulatory response by the T cell, such as, but not limited to, proliferation. Costimulatory molecules include, but are not limited to, MHC class I molecules, BTLA, and Toll ligand receptors.

[0056] A "costimulatory signal," as used herein, refers to a signal that, in combination with a primary signal, such as TCR / CD3 ligation, leads to T cell proliferation and / or up- or down-regulation of key molecules.

[0057] A "disease" is a state of health in an animal in which the animal is unable to maintain homeostasis and in which the animal's health will continue to deteriorate if the disease is not ameliorated. In contrast, a "disorder" in an animal is a state of health in which the animal is able to maintain homeostasis, but in which the animal's health is less favorable than it would be in the absence of the disorder. If left untreated, a disorder does not necessarily cause a further deterioration in the animal's health.

[0058] The term "downregulation," as used herein, refers to a decrease or elimination of gene expression of one or more genes.

[0059] "Effective amount" or "therapeutically effective amount" are used interchangeably herein and refer to an amount of a compound, formulation, material or composition described herein that is effective to achieve a particular biological result or provide a therapeutic or prophylactic benefit. Such results may include, but are not limited to, an amount that, when administered to a mammal, causes a detectable level of immune suppression or tolerance compared to the immune response detected in the absence of the composition of the present invention. The immune response can be readily assessed by a myriad of art-recognized methods. Those skilled in the art will understand that the amount of the composition administered herein will vary and can be readily determined based on a number of factors, such as the disease or condition being treated, the age and health and physical condition of the mammal being treated, the severity of the disease, the particular compound being administered, and the like.

[0060] "Encoding" refers to the inherent property of a particular nucleotide sequence in a polynucleotide, such as a gene, cDNA, or mRNA, to serve as a template for the synthesis of other polymers and macromolecules in biological processes, either having a defined nucleotide (i.e., rRNA, tRNA, and mRNA) sequence or a defined amino acid sequence, and the biological properties resulting therefrom. Thus, a gene encodes a protein when the protein is produced in a cell or other biological system by transcription and translation of the mRNA corresponding to that gene. Both the coding strand, the nucleotide sequence of which is identical to the mRNA sequence and which is usually shown in a sequence listing, and the non-coding strand, which is used as a template for transcription of the gene or cDNA, can be said to encode the protein or other product of that gene or cDNA.

[0061] As used herein, "endogenous" refers to any material that originates or is produced within an organism, cell, tissue or system.

[0062] The term "epitope" as used herein is defined as a small chemical molecule on an antigen that can elicit an immune response and induce a B-cell response and / or a T-cell response. An antigen can have one or more epitopes. Most antigens have many epitopes; that is, they are multivalent. In general, an epitope is approximately the size of about 10 amino acids and / or sugars. Preferably, an epitope is about 4-18 amino acids, more preferably about 5-16 amino acids, even more preferably about 6-14 amino acids, more preferably about 7-12, and most preferably about 8-10 amino acids. In general, one skilled in the art will understand that the overall three-dimensional structure of the molecule, rather than the specific linear sequence, is the primary criterion for the specificity of an antigen, and thus, this distinguishes one epitope from another. Based on the present disclosure, a peptide used in the present invention can be an epitope.

[0063] As used herein, the term "exogenous" refers to any material that is introduced from or produced outside an organism, cell, tissue or system.

[0064] The term "expanded" as used herein refers to an increase in number, such as an increase in the number of T cells. In one embodiment, ex vivo expanded T cells are increased in number relative to the number originally present in the culture. In another embodiment, ex vivo expanded T cells are increased in number relative to other cell types in the culture. As used herein, the term "ex vivo" refers to cells removed from an organism (e.g., a human) and propagated outside the organism (e.g., in a culture dish, test tube, or bioreactor).

[0065] The term "expression" as used herein is defined as the transcription and / or translation of a particular nucleotide sequence driven by its promoter.

[0066] "Expression vector" refers to a vector that contains a recombinant polynucleotide that contains an expression control sequence operably linked to the nucleotide sequence to be expressed. An expression vector contains sufficient cis-acting elements for expression; other elements for expression can be supplied by the host cell or in an in vitro expression system. Expression vectors include all those known in the art, such as cosmids, plasmids (e.g., naked or contained in liposomes) and viruses (e.g., Sendai virus, lentivirus, retrovirus, adenovirus and adeno-associated virus) that incorporate a recombinant polynucleotide.

[0067] "Identity" as used herein refers to the identity of subunit sequences between two amino acid molecules, such as between two polymer molecules, particularly between two polypeptide molecules. If two amino acid sequences have the same residue at the same position; for example, if a position in each of the two polypeptide molecules is occupied by arginine, they are identical at that position. The identity or degree to which two amino acid sequences have the same residue at the same position in an alignment is often expressed as a percentage. The identity between two amino acid sequences is a direct function of the number of positions that are matched or identical; for example, if half of the positions in the two sequences (e.g., 5 positions in a 10 amino acid long polymer) are identical, the two sequences are 50% identical; if 90% of the positions (e.g., 9 out of 10) are matched or identical, the two amino acid sequences are 90% identical.

[0068] As used herein, the term "immune response" is defined as a cellular response to an antigen that occurs when lymphocytes identify the antigen molecule as foreign and induce the formation of antibodies and / or activate lymphocytes to eliminate the antigen.

[0069] The term "immunosuppression" is used herein to refer to the overall reduction of the immune response.

[0070] "Isolated" means altered or removed from the natural state. For example, a nucleic acid or peptide that is naturally present in a living animal is not "isolated," but the same nucleic acid or peptide that is partially or completely separated from the coexisting materials of its natural state is "isolated." An isolated nucleic acid or protein can exist in a substantially purified form, or can exist in a non-native environment, such as, for example, a host cell.

[0071] "Lentivirus" as used herein refers to a genus of the Retroviridae family.Lentiviruses are unique among retroviruses in that they can infect non-dividing cells; they can deliver a significant amount of genetic information into the DNA of host cells, making them one of the most efficient methods of gene delivery vectors.HIV, SIV and FIV are all examples of lentiviruses.Vectors derived from lentiviruses provide a means to achieve significant levels of gene transfer in vivo.

[0072] The term "modified" as used herein refers to an altered state or structure of a molecule or cell of the invention. Molecules can be modified in many ways, including chemically, structurally, and functionally. Cells can be modified by the introduction of nucleic acids.

[0073] The term "modulate" as used herein means to mediate a detectable increase or decrease in the level of response in a subject, compared to the level of response in the subject in the absence of treatment or compound, and / or compared to the level of response in an otherwise identical but untreated subject. This term encompasses perturbing and / or affecting a natural signal or response in a subject, preferably a human, thereby mediating a beneficial therapeutic response.

[0074] In the context of the present invention, the following abbreviations for commonly occurring nucleobases are used: "A" refers to adenosine, "C" refers to cytosine, "G" refers to guanosine, "T" refers to thymidine, and "U" refers to uridine.

[0075] The term "oligonucleotide" typically refers to a short polynucleotide. When a nucleotide sequence is represented by a DNA sequence (i.e., A, T, C, G), it is understood that this nucleotide sequence also includes an RNA sequence (i.e., A, U, C, G) (where "U" is replaced by "T").

[0076] Unless otherwise specified, a "nucleotide sequence encoding an amino acid sequence" includes all nucleotide sequences that are degenerate versions of each other and that encode the same amino acid sequence. The phrase nucleotide sequence encoding a protein or RNA can also include introns, to the extent that the nucleotide sequence encoding the protein may, in some version, contain introns.

[0077] "Parenteral" administration of the immunogenic compositions includes, for example, subcutaneous (sc), intravenous (iv), intramuscular (im), or intrasternal injection, or infusion techniques.

[0078] The term "polynucleotide" as used herein is defined as a chain of nucleotides. Furthermore, nucleic acid is a polymer of nucleotides. Thus, nucleic acid and polynucleotide as used herein are interchangeable. Those skilled in the art have the general knowledge that nucleic acids are polynucleotides and they can be hydrolyzed into monomeric "nucleotides". Monomeric nucleotides can be hydrolyzed into nucleosides. As used herein, polynucleotide includes, but is not limited to, all nucleic acid sequences obtained by any means available in the art, including recombinant means, i.e., cloning nucleic acid sequences from recombinant libraries or cell genomes using conventional cloning techniques and PCR, etc., as well as synthetic means.

[0079] As used herein, the terms "peptide", "polypeptide" and "protein" are used interchangeably and refer to a compound composed of amino acid residues covalently linked by peptide bonds. A protein or peptide must contain at least two amino acids, and there is no limit to the maximum number of amino acids that may make up a protein or peptide sequence. A polypeptide includes any peptide or protein that contains two or more amino acids joined together by peptide bonds. As used herein, the term refers to both short chains, also commonly referred to in the art as peptides, oligopeptides and oligomers, for example, and longer chains, also commonly referred to in the art as proteins, of which there are many types. "Polypeptides" include, inter alia, for example, biologically active fragments, substantially homologous polypeptides, oligopeptides, homodimers, heterodimers, variants of polypeptides, modified polypeptides, derivatives, analogs, fusion proteins. A polypeptide includes natural peptides, recombinant peptides, synthetic peptides, or combinations thereof.

[0080] The term "specifically binds" as used herein with respect to an antibody refers to an antibody that recognizes a specific antigen but does not substantially recognize or bind to other molecules in a sample. For example, an antibody that specifically binds to an antigen from one species may also bind to that antigen from one or more species. However, such cross-species reactivity does not, in and of itself, change the classification of the antibody as specific. In another example, an antibody that specifically binds to an antigen may also bind to different allelic forms of that antigen. However, such cross-reactivity does not, in and of itself, change the classification of the antibody as specific. In some cases, the terms "specific binding" or "specifically binds" can be used in relation to the interaction of an antibody, protein or peptide with a second chemical species to mean that the interaction is dependent on the presence of a particular structure (e.g., an antigenic determinant or epitope) on the chemical species; for example, an antibody recognizes and binds to a particular protein structure rather than the entire protein. If an antibody is specific for epitope "A", then the presence of a molecule containing epitope A (or free, unlabeled A) in a reaction containing labeled "A" and the antibody will reduce the amount of labeled A bound to the antibody.

[0081] The term "stimulation" refers to a primary response induced by the binding of a stimulatory molecule (e.g., the TCR / CD3 complex) to its cognate ligand, thereby mediating a signal transduction event, such as, but not limited to, signal transduction through the TCR / CD3 complex. Stimulation can mediate changes in the expression of certain molecules, such as downregulation of TGF-beta and / or rearrangement of cytoskeletal structures.

[0082] "Stimulatory molecule," as that term is used herein, means a molecule on a T cell that specifically binds to a cognate stimulatory ligand present on an antigen-presenting cell.

[0083] As used herein, a "stimulatory ligand" refers to a ligand that, when present on an antigen-presenting cell (e.g., aAPC, dendritic cell, B cell, etc.), can specifically bind to a cognate binding partner (referred to herein as a "stimulatory molecule") on a T cell, thereby mediating a primary response by the T cell, including, but not limited to, activation, initiation of an immune response, proliferation, etc. Stimulatory ligands are well known in the art and include peptide-loaded MHC class I molecules, anti-CD3 antibodies, superagonist anti-CD28 antibodies, and superagonist anti-CD2 antibodies, among others.

[0084] The term "subject" is intended to include organisms (e.g., mammals) in which an immune response can be elicited. As used herein, a "subject" or "patient" can be a human or non-human mammal. Non-human mammals include farm animals and pets, such as, for example, ovine, bovine, porcine, canine, feline and murine mammals. Preferably, the subject is a human.

[0085] "Target site" or "target sequence" refers to a nucleic acid sequence that defines a portion of a nucleic acid to which a binding molecule can specifically bind under conditions sufficient for binding to occur. In some embodiments, a target sequence refers to a genomic nucleic acid sequence that defines a portion of a nucleic acid to which a binding molecule can specifically bind under conditions sufficient for binding to occur.

[0086] As used herein, the term "T cell receptor" or "TCR" refers to a complex of membrane proteins involved in the activation of T cells in response to the presentation of antigen. TCRs are responsible for recognizing antigens bound to major histocompatibility complex molecules. TCRs are composed of a heterodimer of alpha (a) and beta (β) chains, although in some cells, TCRs consist of gamma and delta (γ / δ) chains. TCRs can exist in alpha / beta and gamma / delta forms, which are structurally similar but have different anatomical locations and functions. Each chain is composed of two extracellular domains, a variable domain and a constant domain. In some embodiments, TCRs can be engineered on any cell that contains a TCR, including, for example, helper T cells, cytotoxic T cells, memory T cells, regulatory T cells, natural killer T cells, and gamma delta T cells.

[0087] The term "therapeutic" as used herein means treatment and / or prophylaxis. The therapeutic effect is achieved by suppression, amelioration, or eradication of the disease state.

[0088] "Graft" refers to a biocompatible lattice or donor tissue, organ or cells to be transplanted. Examples of grafts may include, but are not limited to, skin cells or tissue, bone marrow and solid organs such as the heart, pancreas, kidney, lung and liver. Grafts can also refer to any material to be administered to a host. For example, grafts can refer to nucleic acids or proteins.

[0089] As used herein, the terms "transfected" or "transformed" or "transduced" refer to the process by which exogenous nucleic acid is transferred or introduced into a host cell. A "transfected" or "transformed" or "transduced" cell is one that has been transfected, transformed or transduced with exogenous nucleic acid. This cell includes the primary subject cell and its progeny.

[0090] "Treating" a disease, as that term is used herein, means reducing the frequency or severity of at least one sign or symptom of the disease or disorder from which a subject suffers.

[0091] A "vector" is a composition of material that contains an isolated nucleic acid and can be used to deliver the isolated nucleic acid to the inside of a cell. Numerous vectors are known in the art, including but not limited to linear polynucleotides, polynucleotides associated with ionic or amphiphilic compounds, plasmids and viruses. Thus, the term "vector" includes autonomously replicating plasmids or viruses. This term should also be construed to include non-plasmid and non-viral compounds that facilitate the transfer of nucleic acid into cells, such as polylysine compounds, liposomes, etc. Examples of viral vectors include but are not limited to Sendai virus vectors, adenovirus vectors, adeno-associated virus vectors, retrovirus vectors, lentivirus vectors, etc.

[0092] Ranges: Throughout this disclosure, various aspects of the invention can be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Thus, the description of a range should be considered to have specifically disclosed all possible subranges and individual numerical values ​​within that range. For example, the description of a range such as 1-6 should be considered to have specifically disclosed subranges such as 1-3, 1-4, 1-5, 2-4, 2-6, 3-6, etc., as well as individual numbers within that range, for example, 1, 2, 2.7, 3, 4, 5, 5.3, and 6. This applies regardless of the breadth of the range.

[0093] B. Chimeric Antigen Receptors The present invention provides a chimeric antigen receptor (CAR) that can bind to prostate stem cell antigen (PSCA).In a particular embodiment, the subject CAR comprises an antigen binding domain that can bind to PSCA, a transmembrane domain, and an intracellular domain.In another aspect, the present invention comprises a bispecific CAR, comprising an extracellular domain that comprises an antigen binding domain that can bind to prostate stem cell antigen (PSCA) and an antigen binding domain that can bind to prostate specific membrane antigen (PSMA), a transmembrane domain, and an intracellular domain.

[0094] Also provided are compositions and methods for modified immune cells or their precursors (e.g., modified T cells) comprising CAR. Thus, in some embodiments, immune cells are genetically modified to express CAR. Also provided are nucleic acids encoding the CAR, vectors encoding the nucleic acids, and modified cells (e.g., modified T cells) comprising the CAR, vector, or nucleic acid.

[0095] The subject CAR of the present invention comprises an antigen-binding domain capable of binding to PSCA, a transmembrane domain, and an intracellular domain. The subject CAR of the present invention may optionally comprise a hinge domain. Thus, the subject CAR of the present invention comprises an antigen-binding domain capable of binding to PSCA, a hinge domain, a transmembrane domain, and an intracellular domain. The subject bispecific CAR of the present invention comprises an extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen-binding domain capable of binding to prostate specific membrane antigen (PSMA). In an embodiment in which the CAR is a bispecific CAR, the antigen-binding domain capable of binding to PSCA is linked to the antigen-binding domain capable of binding to PSMA. In some embodiments, the PSCA antigen-binding domain can be N-terminal to the PSMA antigen-binding domain. In some embodiments, the PSMA antigen-binding domain can be N-terminal to the PSCA antigen-binding domain. The C-terminal antigen-binding domain of the bispecific CAR of the present invention can be functionally linked to another domain of the CAR as described herein.

[0096] The antigen binding domain can be operably linked to another domain of the CAR, such as a transmembrane domain or an intracellular domain, both of which are described elsewhere herein, for expression in a cell. In one embodiment, a first nucleic acid sequence encoding the antigen binding domain is operably linked to a second nucleic acid encoding the transmembrane domain, which is further operably linked to a third nucleic acid sequence encoding the intracellular domain.

[0097] The antigen binding domain described herein can be combined with any of the transmembrane domains described herein, any of the intracellular or cytoplasmic domains described herein, or any of the other domains described herein that can be included in the CAR of the present invention.The subject CAR of the present invention can also include a hinge domain as described herein.The subject CAR of the present invention can also include a spacer domain as described herein.In some embodiments, each of the antigen binding domain, the transmembrane domain, and the intracellular domain is separated by a linker.

[0098] Antigen-binding domain The antigen-binding domain of the CAR is the extracellular region of the CAR that binds to a specific target antigen, including proteins, sugars, and glycolipids. The subject CAR of the present invention comprises an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA).

[0099] The antigen-binding domain can include any domain that binds to an antigen, including, but not limited to, monoclonal antibodies, polyclonal antibodies, synthetic antibodies, human antibodies, humanized antibodies, non-human antibodies, and any fragments thereof. In some embodiments, the antigen-binding domain portion comprises a mammalian antibody or a fragment thereof. The choice of antigen-binding domain can depend on the type and number of antigens present on the surface of the target cell.

[0100] In some embodiments, the antigen-binding domain is selected from the group consisting of an antibody, an antigen-binding fragment (Fab), and a single chain variable fragment (scFv). In some embodiments, the PSCA-binding domain of the present invention is selected from the group consisting of a PSCA-specific antibody, a PSCA-specific Fab, and a PSCA-specific scFv. In one embodiment, the PSCA-binding domain is a PSCA-specific antibody. In one embodiment, the PSCA-binding domain is a PSCA-specific Fab. In one embodiment, the PSCA-binding domain is a PSCA-specific scFv.

[0101] As used herein, the term "single-chain variable fragment" or "scFv" refers to a fusion protein in which the variable regions of the heavy (VH) and light (VL) chains of an immunoglobulin (e.g., mouse or human) are covalently linked to form a VH::VL heterodimer. The heavy (VH) and light (VL) chains are either directly linked or linked by a peptide-encoded linker connecting the N-terminus of the VH to the C-terminus of the VL or the C-terminus of the VH to the N-terminus of the VL. In some embodiments, the antigen-binding domain (e.g., PSCA binding domain) comprises an scFv having, from the N-terminus to the C-terminus, the VH-linker-VL configuration. In some embodiments, the antigen-binding domain comprises an scFv having, from the N-terminus to the C-terminus, the VL-linker-VH configuration. Those skilled in the art will be able to select a configuration suitable for use in the present invention.

[0102] The linker is usually glycine-rich for flexibility, as well as serine- or threonine-rich for solubility. The linker can link the heavy chain variable region and the light chain variable region of the extracellular antigen-binding domain. Non-limiting examples of linkers are disclosed in Shen et al., Anal. Chem. 80(6):1910-1917 (2008) and WO 2014 / 087010, the contents of which are incorporated herein by reference in their entirety. (GS) n , (GSGGS) n (SEQ ID NO:1), (GGGS) n (SEQ ID NO:2), and (GGGGS)n A variety of linker sequences are known in the art, including, but not limited to, glycine serine (GS) linkers, such as (SEQ ID NO:3), where n represents an integer of at least 1. Exemplary linker sequences include: The linker sequence may include, but is not limited to, the amino acid sequence of the nucleic ... Amino acid sequence that can be encoded by TIFF0007682859000002.tif4164 TIFF0007682859000003.tif7156.

[0103] Despite the removal of the constant region and the introduction of the linker, the scFv protein retains the specificity of the original immunoglobulin. Single chain Fv polypeptide antibodies can be expressed from nucleic acids containing VH and VL coding sequences as described by Huston et al. (Proc. Nat. Acad. Sci. USA, 85:5879-5883, 1988). See also U.S. Patent Nos. 5,091,513, 5,132,405 and 4,956,778; and U.S. Patent Application Publication Nos. 20050196754 and 20050196754. Antagonistic scFvs with inhibitory activity have been described (e.g., Zhao et al., Hyrbidoma (Larchmt) 2008 27(6):455-51; Peter et al., J Cachexia Sarcopenia Muscle 2012 August 12; Shieh et al., J Imunol 2009 183(4):2277-85; Giomarelli et al., Thromb Haemost 2007 97(6):955-63; Fife et al., J Clin Invst 2006 116(8):2252-61; Brocks et al., Immunotechnology 1997 3(3):173-84; Moosmayer et al., Ther Immunol 1995 136(8):2252-61). 2 (10:31-40). Agonistic scFvs with stimulatory activity have been described (see, e.g., Peter et al., J Bioi Chem 2003 25278(38):36740-7; Xie et al., Nat Biotech 1997 15(8):768-71; Ledbetter et al., Crit Rev Immunol 1997 17(5-6):427-55; Ho et al., BioChim Biophys Acta 2003 1638(3):257-66).

[0104] As used herein, "Fab" refers to the fragment of an antibody structure that binds an antigen but is monovalent and does not have the Fc portion; for example, digestion of an antibody with the enzyme papain results in two Fab fragments and one Fc fragment (e.g., heavy (H) chain constant region; the Fc region that does not bind antigen).

[0105] As used herein, "F(ab')2" refers to an antibody fragment produced by pepsin digestion of a whole IgG antibody, where the fragment has two antigen-binding (ab') (bivalent) regions, each (ab') region containing two separate amino acid chains linked by S-S bonds, a portion of a heavy chain for binding to antigen and a light (L) chain, with the remaining heavy chain portions linked together. The "F(ab')2" fragment can be split into two separate Fab' fragments.

[0106] In some embodiments, the antigen binding domain may be derived from the same species as the species that the CAR will ultimately be used in. For example, for use in humans, the antigen binding domain of the CAR may comprise a human antibody or a fragment thereof. In some embodiments, the antigen binding domain may be derived from a different species that the CAR will ultimately be used in. For example, for use in humans, the antigen binding domain of the CAR may comprise a mouse antibody or a fragment thereof.

[0107] In certain embodiments, the antigen binding domain capable of binding to prostate stem cell antigen (PSCA) comprises a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs). HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3). The antigen binding domain capable of binding to prostate stem cell antigen (PSCA) also comprises a light chain variable region comprising three light chain complementarity determining regions (LCDRs). In certain embodiments, LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6).

[0108] In certain embodiments, the heavy chain variable region (VH) of the antigen binding domain capable of binding to prostate stem cell antigen (PSCA) comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, and / or the light chain variable region (VH) comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8.

[0109] In certain embodiments, the antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) is selected from the group consisting of a full-length antibody or antigen-binding fragment thereof, a Fab, a single-chain variable fragment (scFv), or a single-domain antibody. In certain embodiments, the antigen-binding domain is a single-chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9.

[0110] Acceptable variations of antigen binding domain sequences will be known to those of skill in the art. For example, in some embodiments, an antigen binding domain capable of binding to prostate stem cell antigen (PSCA) comprises an amino acid sequence having at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to any of the amino acid sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, or 9.

[0111] In some embodiments, the CAR of the present disclosure may have affinity for one or more target antigens on one or more target cells. In some embodiments, the CAR may have affinity for one or more target antigens on a target cell. In such embodiments, the CAR is a bispecific CAR or a multispecific CAR. In some embodiments, the CAR comprises one or more target-specific binding domains that confer affinity for one or more target antigens. In some embodiments, the CAR comprises one or more target-specific binding domains that confer affinity for the same target antigen. For example, a CAR that comprises one or more target-specific binding domains that have affinity for the same target antigen can also bind to separate epitopes of the target antigen. When there are multiple target-specific binding domains in a CAR, the binding domains may be tandemly aligned or separated by a linker peptide. For example, in a CAR that comprises two target-specific binding domains, the binding domains are covalently connected to each other on a single polypeptide chain through an oligo- or polypeptide linker, an Fc hinge region, or a membrane hinge region.

[0112] In certain aspects, the present invention includes a bispecific CAR comprising an extracellular domain comprising an antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen binding domain capable of binding to prostate specific membrane antigen (PSMA), a transmembrane domain, and an intracellular domain.

[0113] In certain embodiments, an antigen binding domain capable of binding to PSCA comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The antigen binding domain capable of binding to PSMA comprises a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0114] In certain embodiments, an antigen binding domain capable of binding to PSCA comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). and an antigen binding domain capable of binding to PSMA comprising a first light chain variable region comprising the amino acid sequence EYTIH (SEQ ID NO:68), HCDR2 comprising the amino acid sequence NINPNNGGTTYNQKFED (SEQ ID NO:69), and HCDR3 comprising the amino acid sequence GWNFDY (SEQ ID NO:70); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence KASQDVGTAVD (SEQ ID NO:71), LCDR2 comprises the amino acid sequence WASTRHT (SEQ ID NO:72), and LCDR3 comprises the amino acid sequence QQYNSYPLT (SEQ ID NO:73).

[0115] In certain embodiments, the bispecific CAR comprises a first heavy chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, a first light chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8, and / or a second heavy chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34, and a second light chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:35. and a second light chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:35.

[0116] In certain embodiments, the bispecific CAR comprises a first heavy chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, a first light chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8, and / or a second heavy chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75, and a second light chain variable region comprising an amino acid sequence at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:76. and a second light chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:77.

[0117] In certain embodiments, the antigen binding domain capable of binding to PSCA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9, and / or the antigen binding domain capable of binding to PSMA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:36, 78, 84 or 85.

[0118] In certain embodiments, the bispecific CAR comprises an extracellular domain comprising an antigen binding domain capable of binding to PSCA and an antigen binding domain capable of binding to PSMA, wherein the extracellular domain comprises an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to the amino acid sequence set forth in SEQ ID NO:50 or 52.

[0119] In certain embodiments, the antigen-binding domain capable of binding to PSCA and the antigen-binding domain capable of binding to PSMA are separated by a linker.Any linker known in the art may be used to separate the antigen-binding domain capable of binding to PSCA and the antigen-binding domain capable of binding to PSMA.In certain embodiments, the linker comprises the amino acid sequence shown in SEQ ID NO:37 or 38.

[0120] Additional PSMA-binding agents and CARs are described in PCT / US2019 / 020729, the contents of which are incorporated herein by reference in their entirety. In certain embodiments, the antigen-binding domain capable of binding to PSMA comprises a heavy chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75. In certain embodiments, the antigen-binding domain capable of binding to PSMA comprises a light chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:77. In certain embodiments, the antigen binding domain capable of binding to PSMA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:36, 78 or 84.

[0121] In certain embodiments, the antigen-binding domain capable of binding to PSMA comprises any of the heavy and light chain variable regions disclosed in PCT Patent Publication Nos. WO2017212250A1 and WO2018033749A1, the disclosures of which are incorporated herein by reference in their entirety. For example, the antigen-binding domain capable of binding to PSMA can comprise an scFv comprising any of the heavy and light chain variable regions disclosed therein. Thus, the bispecific CAR or PSMA-CAR of the present invention comprises an antigen-binding domain capable of binding to PSMA, which can comprise any scFv and any of the heavy and light chain variable regions as disclosed in WO2017212250A1 and WO2018033749A1.

[0122] In certain embodiments, an antigen binding domain capable of binding to PSMA can comprise any of the heavy and light chain variable regions shown in Table 1.

[0123] (Table 1) TIFF0007682859000004.tif93166TIFF0007682859000005.tif192166TIFF0007682859000006.tif221166

[0124] Transmembrane domain The CAR of the present invention (including bispecific CAR) may comprise a transmembrane domain that connects the antigen-binding domain of the CAR with the intracellular domain of the CAR. The transmembrane domain of the subject CAR is a region that can penetrate the plasma membrane of a cell (e.g., an immune cell or its precursor). The transmembrane domain is for insertion into a cell membrane, e.g., a eukaryotic cell membrane. In some embodiments, the transmembrane domain is sandwiched between the antigen-binding domain and the intracellular domain of the CAR.

[0125] In some embodiments, the transmembrane domain is naturally associated with one or more of the domains in the CAR. In some embodiments, the transmembrane domain can be selected or modified by one or more amino acid substitutions to avoid binding of such domains with the transmembrane domains of the same or different surface membrane proteins, thereby minimizing interaction with other members of the receptor complex.

[0126] The transmembrane domain may be derived from either natural or synthetic sources.When the source is natural, the domain can be derived from any membrane-binding or transmembrane protein, for example, type I transmembrane protein.When the source is synthetic, the transmembrane domain can be any artificial sequence, for example, artificial hydrophobic sequence, that facilitates the insertion of CAR into cell membrane. Examples of transmembrane domains of particular use in the present invention include, but are not limited to, transmembrane domains derived from (i.e. comprising at least the transmembrane region of) the alpha, beta or zeta chain of the T-cell receptor, CD28, CD3 epsilon, CD45, CD4, CD5, CD7, CD8, CD9, CD16, CD22, CD33, CD37, CD64, CD80, CD86, CD134 (OX-40), CD137 (4-1BB), CD154 (CD40L), ICOS (CD278), Toll-like receptor 1 (TLR1), TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8 and TLR9, or transmembrane domains derived from killer cell immunoglobulin-like receptors (KIR).

[0127] In certain embodiments, the transmembrane domain comprises the transmembrane domain of CD8. In certain embodiments, the transmembrane domain of CD8 is the transmembrane domain of CD8 alpha. In certain embodiments, the transmembrane domain of CD8 comprises the amino acid sequence set forth in SEQ ID NO:11. In certain embodiments, the transmembrane domain comprises the transmembrane domain of CD28. In certain embodiments, the transmembrane domain of CD28 comprises the amino acid sequence set forth in SEQ ID NO:12. In certain embodiments, the transmembrane domain comprises the transmembrane domain of ICOS. In certain embodiments, the transmembrane domain of ICOS comprises the amino acid sequence set forth in SEQ ID NO:13.

[0128] In some embodiments, the transmembrane domain may be synthetic, in which case it will comprise primarily hydrophobic residues such as leucine and valine. Preferably, a triplet of phenylalanine, tryptophan and valine will be found at each end of a synthetic transmembrane domain.

[0129] The transmembrane domains described herein can be combined with any of the antigen binding domains described herein, any of the intracellular domains described herein, or any of the other domains described herein that can be included in the subject CAR.

[0130] In some embodiments, the transmembrane domain further comprises a hinge region. The CAR of the subject invention may also comprise a hinge region. The hinge region of the CAR is a hydrophilic region located between the antigen binding domain and the transmembrane domain. In some embodiments, this domain facilitates proper protein folding for the CAR. The hinge region is an optional component of the CAR. The hinge region may comprise a domain selected from an antibody Fc fragment, an antibody hinge region, an antibody CH2 region, an antibody CH3 region, an artificial hinge sequence, or a combination thereof. Examples of hinge regions include, but are not limited to, the CD8a hinge, an artificial hinge composed of a polypeptide that may be as small as three glycines (Gly), and the CH1 and CH3 domains of IgG (such as human IgG4).

[0131] In some embodiments, the subject CARs (including bispecific CARs) of the present disclosure comprise a hinge region that connects the antigen-binding domain to the transmembrane domain, which in turn connects it to the intracellular domain. The hinge region is preferably capable of assisting the antigen-binding domain in recognizing and binding to the target antigen on the target cell (see, e.g., Hudecek et al., Cancer Immunol. Res. (2015) 3(2): 125-135). In some embodiments, the hinge region is a flexible domain, thereby allowing the antigen-binding domain to have a structure that optimally recognizes the specific structure and density of the target antigen on a cell, such as a tumor cell (Hudecek et al., supra). The flexibility of the hinge region allows it to adopt many different conformations.

[0132] In some embodiments, the hinge region is an immunoglobulin heavy chain hinge region. In some embodiments, the hinge region is a receptor-derived hinge region polypeptide (e.g., a CD8-derived hinge region).

[0133] The hinge region can have a length of about 4 amino acids to about 50 amino acids, for example, about 4 aa to about 10 aa, about 10 aa to about 15 aa, about 15 aa to about 20 aa, about 20 aa to about 25 aa, about 25 aa to about 30 aa, about 30 aa to about 40 aa, or about 40 aa to about 50 aa. In some embodiments, the hinge region can have a length of more than 5 aa, more than 10 aa, more than 15 aa, more than 20 aa, more than 25 aa, more than 30 aa, more than 35 aa, more than 40 aa, more than 45 aa, more than 50 aa, more than 55 aa, or more.

[0134] A suitable hinge region can be readily selected and can be any of a number of suitable lengths, for example, 1 amino acid (e.g., Gly) to 20 amino acids, 2 to 15 amino acids, 3 to 12 amino acids, including 4 to 10 amino acids, 5 to 9 amino acids, 6 to 8 amino acids, or 7 to 8 amino acids, and can be 1, 2, 3, 4, 5, 6, or 7 amino acids. Suitable hinge regions can have a length of more than 20 amino acids (e.g., 30, 40, 50, 60, or more).

[0135] For example, the hinge region may be a glycine polymer (G) n , glycine-serine polymers (e.g., (GS) n , (GSGGS) n (SEQ ID NO: 119) and (GGGS) n(SEQ ID NO:120), where n is an integer of at least 1), glycine-alanine polymers, alanine-serine polymers, and other flexible linkers known in the art. Glycine and glycine-serine polymers can be used; both Gly and Ser are relatively unstructured and therefore can serve as neutral tethers between components. Glycine polymers can be used; glycine has significantly more access to the φ-ψ space than alanine and is much less restricted than residues with longer side chains (see, e.g., Scheraga, Rev. Computational. Chem. (1992) 2: 73-142). Exemplary hinge regions include, but are not limited to: It may contain amino acid sequences including TIFF0007682859000007.tif18162, etc.

[0136] In some embodiments, the hinge region is an immunoglobulin heavy chain hinge region. The amino acid sequences of immunoglobulin hinge regions are known in the art; see, for example, Tan et al., Proc. Natl. Acad. Sci. USA (1990) 87(1):162-166; and Huck et al., Nucleic Acids Res. (1986) 14(4): 1779-1789. As a non-limiting example, an immunoglobulin hinge region may have the following amino acid sequence: TIFF0007682859000008.tif5164 (see, e.g., Glaser et al., J. Biol. Chem. (2005) 280:41494-41503); TIFF0007682859000009.tif34164, etc.

[0137] The hinge region can comprise the amino acid sequence of a human IgG1, IgG2, IgG3 or IgG4 hinge region. In one embodiment, the hinge region can comprise one or more amino acid substitutions and / or insertions and / or deletions compared to a wild-type (naturally occurring) hinge region. For example, His229 of the human IgG1 hinge can be replaced by Tyr, whereby the hinge region comprises the sequence EPKSCDKTYTCPPCP (SEQ ID NO:142); see, for example, Yan et al., J. Biol. Chem. (2012) 287: 5891-5897.

[0138] In certain embodiments, the hinge region can comprise an amino acid sequence derived from human CD8 or a variant thereof. In certain embodiments, the CAR comprises a CD8 alpha hinge sequence comprising the amino acid sequence depicted in SEQ ID NO: 10. In certain embodiments, the CAR comprises a hinge sequence comprising the amino acid sequence depicted in SEQ ID NO: 99. In certain embodiments, the CAR comprises a hinge sequence comprising the amino acid sequence depicted in SEQ ID NO: 100.

[0139] Intracellular domain The subject CARs of the present invention (including the subject bispecific CARs) also comprise an intracellular domain. In certain embodiments, the intracellular domain comprises a costimulatory signaling domain and an intracellular signaling domain. The intracellular domain of the CAR is responsible for activating at least one effector function in the cell (e.g., immune cell) in which the CAR is expressed. The intracellular domain transmits an effector function signal and directs the cell (e.g., immune cell) to perform its specialized function, such as damaging and / or destroying a target cell.

[0140] Examples of intracellular domains for use in the present invention include, but are not limited to, the cytoplasmic portion of a surface receptor, a costimulatory molecule, and any molecules that act in concert to initiate signaling in a T cell, as well as any derivatives or variants of these elements, and any synthetic sequences that have the same functional capabilities.

[0141] Examples of intracellular domains include, but are not limited to, the ζ chain of the T cell receptor complex or any of its homologs, such as the η chain, FcsRI γ and β chains, MB 1 (Iga) chain, B29 (Ig) chain, human CD3 zeta chain, CD3 polypeptides (Δ, δ and ε), syk family tyrosine kinases (Syk, ZAP 70, etc.), src family tyrosine kinases (Lck, Fyn, Lyn, etc.), and other molecules involved in T cell signaling such as CD2, CD5 and CD28. In one embodiment, the intracellular signaling domain can be human CD3 zeta chain, FcyRIII, FcsRI, the cytoplasmic tail of the Fc receptor, immunoreceptor tyrosine-based activation motif (ITAM) bearing cytoplasmic receptors, and combinations thereof.

[0142] In certain embodiments, the intracellular domain of the CAR comprises at least one signaling domain derived from any portion of one or more costimulatory molecules, e.g., CD2, CD3, CD8, CD27, CD28, ICOS (CD278), 4-1BB, PD-1, any derivative or variant thereof, any synthetic sequence thereof having the same functional capability, and any combination thereof. The intracellular domain comprises a costimulatory domain of a protein selected from the group consisting of proteins in the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, and B7-H3 (CD276), or variants thereof, or an intracellular domain derived from a killer immunoglobulin-like receptor (KIR).

[0143] Furthermore, variant intracellular signaling domains suitable for use in the subject CAR are known in the art. The YMFM motif is an SH2 binding motif found in ICOS that recruits both p85 and p50 alpha subunits of PI3K, resulting in enhanced AKT signaling. See, for example, Simpson et al. (2010) Curr. Opin. Immunol., 22:326-332. In one embodiment, CD28 intracellular domain variants can be generated to include YMFM motifs. The YMNM motif is found in CD28 cytoplasmic domain and is a known binding site for phosphatidylinositol 3-kinase (PI3-K) and Grb2. See, Harada et al. (2003) J. Exp. Med., 197(2):257-262. In one embodiment, ICOS intracellular domain variants can be generated to include YMNM motifs.

[0144] In certain embodiments, the intracellular domain comprises a costimulatory domain of 4-1BB. In certain embodiments, the costimulatory domain of 4-1BB comprises the amino acid sequence set forth in SEQ ID NO:14. In certain embodiments, the intracellular domain comprises a costimulatory domain of CD28. In certain embodiments, the costimulatory domain of CD28 comprises the amino acid sequence set forth in SEQ ID NO:15. In certain embodiments, the intracellular domain comprises a costimulatory domain of ICOS. In certain embodiments, the costimulatory domain of ICOS comprises the amino acid sequence set forth in SEQ ID NO:16. In certain embodiments, the intracellular domain comprises a costimulatory domain of ICOS (YMNM). In certain embodiments, the costimulatory domain of ICOS (YMNM) comprises the amino acid sequence set forth in SEQ ID NO:17.

[0145] Other examples of intracellular domains include TCR, CD3 zeta, CD3 gamma, CD3 delta, CD3 epsilon, CD86, common FcR gamma, FcR beta (Fc epsilon Rib), CD79a, CD79b, Fc gamma Rila, DAP10, DAP12, T cell receptor (TCR), CD8, CD27, CD28, 4-1BB (CD137), OX9, OX40, CD30, CD40, PD-1, ICOS, KIR family proteins, lymphocyte function-associated antigen-1 (LFA-1), CD2, CD7, LIGHT, NKG2C, B7-H3, ligand that specifically binds CD83, CDS, ICAM-1, GITR, BAFFR, HVEM (LIGHTR), SLAMF7, NKp80 (KLRF1), CD127, CD160, CD19, CD4, CD8 alpha, CD8 beta, IL2R beta, IL2R gamma, IL7R alpha, ITGA4, VLA1, CD49a, ITGA4, IA4, CD49D, ITGA6, VLA-6, CD49f, ITGAD, CD11d, ITGAE, CD103, ITGAL, CD11a, LFA-1, ITGAM, CDlib, ITGAX, CD11c, ITGBl, CD29, ITGB2, CD18, LFA-1, ITGB7, TNFR2, TRANCE / RANKL, DNAM1 (CD226), SLAMF4 (CD244, 2B4), CD84, CD96 (Tactile), CEACAM1, CRT These include fragments or domains derived from one or more molecules or receptors, including, but not limited to, AM, Ly9 (CD229), CD160 (BY55), PSGL1, CD100 (SEMA4D), CD69, SLAMF6 (NTB-A, Ly108), SLAM (SLAMF1, CD150, IPO-3), BLAME (SLAMF8), SELPLG (CD162), LTBR, ​​LAT, GADS, SLP-76, PAG / Cbp, NKp44, NKp30, NKp46, NKG2D, Toll-like receptor 1 (TLR1), TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, other costimulatory molecules described herein, any derivative, variant, or fragment thereof, any synthetic sequence of a costimulatory molecule having the same functional capability, and any combination thereof.

[0146] Additional examples of intracellular domains include the intracellular signaling domains of several different other immune signaling receptors, including but not limited to, first, second and third generation T cell signaling proteins, including, but not limited to, CD3, B7 family costimulatory receptors and tumor necrosis factor receptor (TNFR) superfamily receptors (see, e.g., Park and Brentjens, J. Clin. Oncol. (2015) 33(6): 651-653). Additionally, the intracellular signaling domain can include signaling domains used by NK cells and NKT cells (see, e.g., Hermanson and Kaufman, Front. Immunol. (2015) 6: 195), such as the signaling domains of NKp30(B7-H6) (see, e.g., Zhang et al., J. Immunol. (2012) 189(5): 2290-2299), and DAP12 (see, e.g., Topfer et al., J. Immunol. (2015) 194(7): 3201-3212), NKG2D, NKp44, NKp46, DAP10, and CD3z.

[0147] In certain embodiments, the intracellular domain comprises an intracellular signaling domain selected from the group consisting of the cytoplasmic signaling domain of human CD3 zeta chain (CD3ζ), FcγRIII, FcsRI, the cytoplasmic tail of Fc receptor, immunoreceptor tyrosine-based activation motif (ITAM) bearing cytoplasmic receptor, TCR zeta, FcR gamma, CD3 gamma, CD3 delta, CD3 epsilon, CD5, CD22, CD79a, CD79b, and CD66d, or variants thereof. In certain embodiments, the intracellular domain comprises the intracellular domain of CD3ζ or variants thereof. In certain embodiments, the intracellular domain of CD3ζ comprises the amino acid sequence shown in 18 or 19.

[0148] Intracellular domains suitable for use in the subject CARs of the present invention include any desired signaling domain that provides a distinct and detectable signal (e.g., increased production of one or more cytokines by the cell; altered transcription of a target gene; altered activity of a protein; altered cellular behavior, e.g., cell death; cell proliferation; cell differentiation; cell survival; modulation of a cell signaling response, etc.) in response to activation of the CAR (i.e., activated by an antigen and a dimerization agent). In some embodiments, the intracellular domain comprises at least one (e.g., one, two, three, four, five, six, etc.) ITAM motif as described below. In some embodiments, the intracellular domain comprises a DAP10 / CD28-type signaling chain. In some embodiments, the intracellular domain is not covalently linked to the membrane-bound CAR, but is instead diffused in the cytoplasm.

[0149] The intracellular domain suitable for use in the subject CAR of the present invention comprises an immunoreceptor tyrosine-based activation motif (ITAM)-containing intracellular signaling polypeptide. In some embodiments, the ITAM motif is repeated twice within the intracellular domain, where the first and second instances of the ITAM motif are separated from each other by 6-8 amino acids. In one embodiment, the intracellular domain of the subject CAR comprises three ITAM motifs.

[0150] In some embodiments, the intracellular domain comprises a signaling domain of a human immunoglobulin receptor containing an immunoreceptor tyrosine-based activation motif (ITAM), such as, but not limited to, Fc gamma RI, Fc gamma RIIA, Fc gamma RIIC, Fc gamma RIIIA, FcRL5 (see, e.g., Gillis et al., Front. Immunol. (2014) 5:254).

[0151] Suitable intracellular domains can be ITAM motif-containing portions derived from ITAM motif-containing polypeptides. For example, suitable intracellular domains can be ITAM motif-containing domains derived from any ITAM motif-containing protein. Thus, suitable intracellular domains do not need to contain the entire sequence of the entire protein from which they are derived. Examples of suitable ITAM motif-containing polypeptides include, but are not limited to, DAP12, FCER1G (Fc epsilon receptor I gamma chain), CD3D (CD3 delta), CD3E (CD3 epsilon), CD3G (CD3 gamma), CD3Z (CD3 zeta), and CD79A (antigen receptor complex-associated protein alpha chain).

[0152] In one embodiment, the intracellular domain is derived from DAP12 (also known as TYROBP; TYRO protein tyrosine kinase binding protein; KARAP; PLOSL; DNAX activating protein 12; KAR associated protein; TYRO protein tyrosine kinase binding protein; killer activating receptor associated protein; killer activating receptor associated protein, etc.). In one embodiment, the intracellular domain is derived from FCER1G (also known as FCRG; Fc epsilon receptor I gamma chain; Fc receptor gamma chain; fc-epsilon RI-gamma; fcR gamma; fceRl gamma; high affinity immunoglobulin epsilon receptor subunit gamma; immunoglobulin E receptor, high affinity gamma chain, etc.). In one aspect, the intracellular domain is derived from the T cell surface glycoprotein CD3 delta chain (also known as CD3D; CD3-DELTA; T3D; CD3 antigen, delta subunit; CD3 delta; CD3d antigen, delta polypeptide (TiT3 complex); OKT3, delta chain; T cell receptor T3 delta chain; T cell surface glycoprotein CD3 delta chain, etc.). In one aspect, the intracellular domain is derived from the T cell surface glycoprotein CD3 epsilon chain (also known as CD3e, T cell surface antigen T3 / Leu-4 epsilon chain, T cell surface glycoprotein CD3 epsilon chain, AI504783, CD3, CD3 epsilon, T3e, etc.). In one aspect, the intracellular domain is derived from the T cell surface glycoprotein CD3 gamma chain (also known as CD3G, T cell receptor T3 gamma chain, CD3-GAMMA, T3G, gamma polypeptide (TiT3 complex), etc.). In one aspect, the intracellular domain is derived from the T cell surface glycoprotein CD3 zeta chain (also known as CD3Z, T cell receptor T3 zeta chain, CD247, CD3-ZETA, CD3H, CD3Q, T3Z, TCRZ, etc.). In one aspect, the intracellular domain is derived from CD79A (also known as B cell antigen receptor complex associated protein alpha chain; CD79a antigen (immunoglobulin-associated alpha); MB-1 membrane glycoprotein; ig-alpha; membrane-bound immunoglobulin-associated protein; surface IgM-associated protein, etc.).In one embodiment, the intracellular domain suitable for use in the FN3 CAR of the present disclosure comprises a DAP10 / CD28 type signaling chain. In one embodiment, the intracellular domain suitable for use in the FN3 CAR of the present disclosure comprises a ZAP70 polypeptide. In some embodiments, the intracellular domain comprises the cytoplasmic signaling domain of TCR zeta, FcR gamma, FcR beta, CD3 gamma, CD3 delta, CD3 epsilon, CD5, CD22, CD79a, CD79b, or CD66d. In one embodiment, the intracellular domain in the CAR comprises the cytoplasmic signaling domain of human CD3 zeta.

[0153] Typically, the entire intracellular domain can be used, but in many cases it is not necessary to use the entire chain. To the extent that a truncated portion of the intracellular domain is used, such a truncated portion can be used in place of the intact chain so long as it transmits an effector function signal. The intracellular domain includes any truncated portion of the intracellular domain sufficient to transmit an effector function signal.

[0154] The intracellular domains described herein can be combined with any of the antigen binding domains described herein, any of the transmembrane domains described herein, or any of the other domains described herein that can be included in a CAR.

[0155] Acceptable variations of individual CAR domain sequences (hinge domain, transmembrane domain, and intracellular domain) will be known to those of skill in the art. For example, in some embodiments, the CAR domain comprises an amino acid sequence having at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to any of the amino acid sequences set forth in SEQ ID NOs: 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 99, and 100.

[0156] In one aspect, the present invention provides a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an antigen binding domain, a transmembrane domain, and an intracellular domain, wherein the antigen binding domain comprises: a heavy chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and a light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8.

[0157] In one aspect, the present invention provides a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9.

[0158] In one aspect, the present invention provides a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 21, 23, 25 or 27. Acceptable variations of CAR sequences will be known to those skilled in the art. For example, in some embodiments, the CAR comprises an amino acid sequence that has at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to any of the amino acid sequences shown in SEQ ID NO: 21, 23, 25 or 27.

[0159] In one aspect, the present invention provides a chimeric antigen receptor (CAR) capable of binding to prostate-specific membrane antigen (PSMA) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 67, 87, 89, 91, 93, 95 or 97. Acceptable variations of the CAR sequence will be known to those of skill in the art. For example, in some embodiments, the CAR comprises an amino acid sequence having at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:67, 87, 89, 91, 93, 95, or 97.

[0160] In one aspect, the present invention provides a bispecific chimeric antigen receptor (CAR), comprising an extracellular domain comprising an antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen binding domain capable of binding to prostate specific membrane antigen (PSMA), a transmembrane domain, and an intracellular domain.

[0161] In one aspect, the invention provides a bispecific CAR, the extracellular domain of which comprises an antigen-binding domain capable of binding to PSCA and an antigen-binding domain capable of binding to PSMA, the bispecific CAR comprising an amino acid sequence as set forth in SEQ ID NO: 40, 42, or 80-82. Acceptable variations of the CAR sequence will be known to those of skill in the art. For example, in some embodiments, the CAR comprises an amino acid sequence having at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to any of the amino acid sequences set forth in SEQ ID NO: 40, 42, or 80-82.

[0162] In one aspect, the present invention provides a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), wherein the antigen binding domain capable of binding to PSMA is a single chain variable fragment (scFv) comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:78, 84 or 85, and / or the antigen binding domain capable of binding to PSCA and the antigen binding domain capable of binding to PSMA comprise the amino acid sequence set forth in SEQ ID NO:50 or 52.

[0163] In one aspect, the present invention provides a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), comprising an extracellular domain, a transmembrane domain, and an intracellular domain, comprising an antigen binding domain capable of binding to PSCA and an antigen binding domain capable of binding to PSMA. The antigen binding domain capable of binding to PSCA comprises a heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, and / or a light chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8. The antigen-binding domain capable of binding to PSMA comprises a heavy chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34, and / or a light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:35.

[0164] In one aspect, the present invention provides a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), wherein the antigen binding domain capable of binding to PSCA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9 and / or the antigen binding domain capable of binding to PSMA is a single chain variable fragment (scFv) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:36.

[0165] In one aspect, the present invention provides a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), comprising an extracellular domain, a transmembrane domain, and an intracellular domain, the extracellular domain comprising an antigen binding domain capable of binding to PSCA and an antigen binding domain capable of binding to PSMA. The antigen binding domain capable of binding to PSCA comprises a heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7, and a light chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8. The antigen-binding domain capable of binding to PSMA comprises a heavy chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75 and a light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:77.

[0166] In one aspect, the invention relates to a method for the production of a polypeptide comprising: a first heavy chain variable region, the extracellular domain of which comprises three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and / or a first light chain variable region, the extracellular domain of which comprises three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). and an antigen binding domain capable of binding to PSMA comprising a first light chain variable region comprising the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprising the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprising the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and / or a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0167] In one aspect, the invention relates to a method for the production of a polypeptide comprising: a first heavy chain variable region, the extracellular domain of which comprises three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and / or a first light chain variable region, the extracellular domain of which comprises three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). and an antigen binding domain capable of binding to PSMA comprising a first light chain variable region comprising the amino acid sequence EYTIH (SEQ ID NO:6), a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence EYTIH (SEQ ID NO:68), HCDR2 comprises the amino acid sequence NINPNNGGTTYNQKFED (SEQ ID NO:69), and HCDR3 comprises the amino acid sequence GWNFDY (SEQ ID NO:70); and / or a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence KASQDVGTAVD (SEQ ID NO:71), LCDR2 comprises the amino acid sequence WASTRHT (SEQ ID NO:72), and LCDR3 comprises the amino acid sequence QQYNSYPLT (SEQ ID NO:73).

[0168] Table 2: Sequences used in the present invention TIFF0007682859000010.tif60169TIFF0007682859000011.tif227169TIFF000768285900001 2.tif225169TIFF0007682859000013.tif229169TIFF0007682859000014.tif226169TIFF0007 682859000015.tif229169TIFF0007682859000016.tif225169TIFF0007682859000017.tif22 5169TIFF0007682859000018.tif229169TIFF0007682859000019.tif225169TIFF00076828590 00020.tif229169TIFF0007682859000021.tif225169TIFF0007682859000022.tif229169TIF F0007682859000023.tif229169TIFF0007682859000024.tif229169TIFF0007682859000025.t if229169TIFF0007682859000026.tif229169TIFF0007682859000027.tif229169TIFF0007682 859000028.tif229169TIFF0007682859000029.tif229169TIFF0007682859000030.tif108169

[0169] C. Nucleic Acids and Expression Vectors The present disclosure provides a nucleic acid encoding a CAR. The nucleic acid of the present disclosure can include a polynucleotide sequence encoding any one of the CARs (including bispecific CARs) disclosed herein.

[0170] In a particular aspect, the present invention includes a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an antigen binding domain, a transmembrane domain, and an intracellular domain. In a particular embodiment, the antigen binding domain comprises a heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), HCDR1 comprising the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprising the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprising the amino acid sequence TGGF (SEQ ID NO:3); and a light chain variable region comprising three light chain complementarity determining regions (LCDRs), LCDR1 comprising the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprising the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprising the amino acid sequence QQWSSSPFT (SEQ ID NO:6).

[0171] In certain embodiments, the antigen binding domain comprises a heavy chain variable region encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43, and / or a light chain variable region encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44.

[0172] In certain embodiments, the antigen binding domain is a single chain variable fragment (scFv) encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:45.

[0173] Also provided is a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising an antigen binding domain, a transmembrane domain, and an intracellular domain, wherein the antigen binding domain comprises a heavy chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and / or a light chain variable region comprising an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8.

[0174] Also provided is a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:20.

[0175] The present invention also provides a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), comprising a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:20, 22, 24 or 26.

[0176] The present invention also provides a nucleic acid comprising a polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to prostate specific membrane antigen (PSMA), wherein the PSMA-CAR comprises an amino acid sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to the amino acid sequence set forth in SEQ ID NO: 67, 87, 89, 91, 93, 95 or 97. In certain embodiments, the PSMA-CAR is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 88, 90, 92, 94, 96 or 98.

[0177] Also provided is a nucleic acid comprising a polynucleotide sequence encoding a bispecific chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA) and prostate specific membrane antigen (PSMA), wherein the bispecific CAR comprises an extracellular domain comprising an antigen binding domain capable of PSCA and an antigen binding domain capable of binding to PSMA, a transmembrane domain, and an intracellular domain.

[0178] In certain embodiments, the extracellular domain comprises an antigen-binding domain capable of binding to PSCA and an antigen-binding domain capable of binding to PSMA. The antigen-binding domain capable of binding to PSCA comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The antigen binding domain capable of binding to PSMA comprises a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0179] In certain embodiments, the extracellular domain comprises an antigen binding domain capable of binding to PSCA and an antigen binding domain capable of binding to PSMA, wherein the antigen binding domain capable of binding to PSCA comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The antigen-binding domain capable of binding to PSMA comprises a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence EYTIH (SEQ ID NO:68), HCDR2 comprises the amino acid sequence NINPNNGGTTYNQKFED (SEQ ID NO:69), and HCDR3 comprises the amino acid sequence GWNFDY (SEQ ID NO:70); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence KASQDVGTAVD (SEQ ID NO:71), LCDR2 comprises the amino acid sequence WASTRHT (SEQ ID NO:72), and LCDR3 comprises the amino acid sequence QQYNSYPLT (SEQ ID NO:73).

[0180] In certain embodiments, the first heavy chain variable region (capable of binding to PSCA) is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:43; the first light chain variable region (capable of binding to PSCA) is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:44, and / or the second heavy chain variable region (capable of binding to PSMA) is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:45. the second light chain variable region (capable of binding to PSMA) is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:46 or 74; and the second light chain variable region (capable of binding to PSMA) is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:47 or 76.

[0181] In certain embodiments, the antigen binding domain capable of binding to PSCA comprises an scFv encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 45. In certain embodiments, the antigen binding domain capable of binding to PSMA comprises an scFv encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO: 48, 83 or 86.

[0182] In certain embodiments, the extracellular domain is encoded by a polynucleotide sequence comprising the nucleic acid sequence shown in SEQ ID NO:49 or 51.

[0183] In certain embodiments, the CAR (including bispecific CAR) further comprises a hinge sequence comprising the amino acid sequence set forth in SEQ ID NO:10, 99 or 100. In certain embodiments, the transmembrane domain of the CAR or bispecific CAR comprises the transmembrane domain of CD8 alpha comprising the amino acid sequence set forth in SEQ ID NO:11. In certain embodiments, the transmembrane domain comprises the transmembrane domain of CD28 comprising the amino acid sequence set forth in SEQ ID NO:12. In certain embodiments, the transmembrane domain comprises the transmembrane domain of ICOS comprising the amino acid sequence set forth in SEQ ID NO:13.

[0184] In certain embodiments, the intracellular domain comprises a costimulatory signaling domain and an intracellular signaling domain. In certain embodiments, the costimulatory signaling domain comprises a costimulatory domain of 4-1BB comprising the amino acid sequence set forth in SEQ ID NO:14. In certain embodiments, the costimulatory signaling domain comprises a costimulatory domain of CD28 comprising the amino acid sequence set forth in SEQ ID NO:15. In certain embodiments, the costimulatory signaling domain comprises a costimulatory domain of ICOS comprising the amino acid sequence set forth in SEQ ID NO:16. In certain embodiments, the costimulatory signaling domain comprises a costimulatory domain of ICOS (YMNM) comprising the amino acid sequence set forth in SEQ ID NO:17. In certain embodiments, the intracellular signaling domain comprises an intracellular domain of CD3zeta or a variant thereof, wherein the intracellular domain of CD3zeta comprises the amino acid sequence set forth in SEQ ID NO:18 or 19.

[0185] In certain embodiments, the bispecific CAR is encoded by a polynucleotide sequence that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:39, 41 or 79.

[0186] Another aspect of the present invention includes a nucleic acid comprising a first polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second polynucleotide sequence encoding a dominant negative receptor, wherein the CAR comprises an antigen binding domain, a transmembrane domain, and an intracellular domain.

[0187] In certain embodiments, the dominant negative receptor is a truncated variant of the wild-type protein associated with negative signaling. In certain embodiments, the truncated variant of the wild-type protein associated with negative signaling comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:56 and / or is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:55.

[0188] Also provided is a nucleic acid comprising a first polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second polynucleotide sequence encoding a switch receptor, wherein the CAR comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain.

[0189] In certain aspects, the switch receptor comprises a first domain derived from a first switch polypeptide associated with a negative signal; and a second domain derived from a second switch polypeptide associated with a positive signal. In certain aspects, the first domain comprises at least a portion of the extracellular domain of the first switch polypeptide associated with a negative signal, and the second domain comprises at least a portion of the intracellular domain of the second switch polypeptide associated with a positive signal.

[0190] In certain embodiments, the switch receptor further comprises a switch receptor transmembrane domain. In certain embodiments, the switch receptor transmembrane domain comprises a transmembrane domain of a first switch polypeptide associated with a negative signal; or a transmembrane domain of a second switch polypeptide associated with a positive signal.

[0191] In certain embodiments, the first switch polypeptide associated with a negative signal is selected from the group consisting of CTLA4, PD-1, PD-L1, BTLA, TIM-3, IFNγR, and TGFβR. In certain embodiments, the first switch polypeptide associated with a positive signal is selected from the group consisting of CD28, ICOS, and IL-12R.

[0192] In certain embodiments, the switch receptor comprises a first domain comprising at least a portion of the extracellular domain of PD1; a switch receptor transmembrane domain comprising at least a portion of the transmembrane domain of CD28; and a second domain comprising at least a portion of the intracellular domain of CD28. The PD1-CD28 switch receptor is described in Liu X, et al. (2016) Cancer research, 76(6), 1578-1590, the contents of which are incorporated herein by reference in their entirety. In certain embodiments, the switch receptor is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:57, and / or comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:58.

[0193] In certain embodiments, the switch receptor comprises a first domain comprising at least a portion of the extracellular domain of IFNγR; and a second domain comprising at least a portion of the intracellular domain of IL12Rβ1. In certain embodiments, the switch receptor is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to the nucleotide sequence set forth in SEQ ID NO:59, and / or comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:60.

[0194] In certain embodiments, the switch receptor comprises a first domain comprising at least a portion of the extracellular domain of IFNγR; and a second domain comprising at least a portion of the intracellular domain of IL12Rβ2. In certain embodiments, the switch receptor is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to the sequence set forth in SEQ ID NO:61, and / or comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:62.

[0195] Additional switch receptors are described in PCT / US2019 / 020729, the contents of which are incorporated by reference in their entirety.

[0196] Another aspect of the invention provides a nucleic acid comprising a first polynucleotide sequence encoding a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second polynucleotide sequence encoding a bispecific antibody, wherein the CAR comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain.

[0197] In certain embodiments, the bispecific antibody comprises a first binding domain and a second binding domain. In certain embodiments, the first binding domain binds to a negative signal selected from the group consisting of CTLA4, PD-1, PD-L1, BTLA, TIM-3, and TGFβR. In certain embodiments, the second binding domain binds to a costimulatory molecule. In certain embodiments, the costimulatory molecule is CD28.

[0198] In certain embodiments, the bispecific antibody comprises a first binding domain capable of binding to PD-L1 and a second binding domain capable of binding to CD28. In certain embodiments, the bispecific antibody is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:63 and / or comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:64.

[0199] In certain embodiments, the bispecific antibody comprises a first binding domain capable of binding to TGFβR2 and a second binding domain capable of binding to CD28. In certain embodiments, the bispecific antibody is encoded by a polynucleotide sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:65 and / or comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:66.

[0200] In certain embodiments, the nucleic acid of the present disclosure comprises a first polynucleotide sequence and a second polynucleotide sequence. The first and second polynucleotide sequences may be separated by a linker. For example, in certain embodiments, the antigen binding domain capable of binding to PSCA and the antigen binding domain capable of binding to PSMA are separated by a linker. In certain embodiments, the linker is encoded by a polynucleotide sequence comprising the nucleic acid sequence shown in SEQ ID NO:53 or 54. The linker for use in the present disclosure allows multiple proteins to be encoded by the same nucleic acid sequence (e.g., multicistronic or bicistronic sequences), which are translated as a polyprotein that is dissociated into separate protein components. For example, the linker for use in the nucleic acid of the present disclosure comprising a PSCA CAR coding sequence and a PSMA CAR coding sequence allows the PSCA CAR and the PSMA CAR to be translated as a polyprotein that is dissociated into separate CARs. In certain embodiments, the nucleic acid comprises, from 5' to 3', a first polynucleotide sequence, a linker, and a second polynucleotide sequence. In certain embodiments, the nucleic acid comprises, from 5' to 3', a second polynucleotide sequence, a linker, and the first polynucleotide sequence.

[0201] In some embodiments, the linker comprises a nucleic acid sequence encoding an internal ribosome entry site (IRES). As used herein, "internal ribosome entry site" or "IRES" refers to an element that promotes direct internal ribosome entry into the initiation codon, such as ATG, of a protein coding region, leading to cap-independent translation of the gene. A variety of internal ribosome entry sites are known to those skilled in the art, including, but not limited to, IRES available from viral or cellular mRNA sources, such as immunoglobulin heavy chain binding protein (BiP); vascular endothelial growth factor (VEGF); fibroblast growth factor 2; insulin-like growth factor; translation initiation factor eIF4G; yeast transcription factors TFIID and HAP4; and IRES available from, for example, cardiovirus, rhinovirus, aphthovirus, HCV, Friend murine leukemia virus (FrMLV), and Moloney murine leukemia virus (MoMLV). Those skilled in the art will be able to select the appropriate IRES for use in the present invention.

[0202] In some embodiments, the linker comprises a nucleic acid sequence encoding a self-cleaving peptide. As used herein, "self-cleaving peptide" or "2A peptide" refers to an oligopeptide that allows multiple proteins to be encoded as a polyprotein, which dissociates into its constituent proteins after translation. The use of the term "self-cleavage" is not intended to imply a proteolytic cleavage reaction. A variety of self-cleaving peptides or 2A peptides are known to those skilled in the art, including, but not limited to, those found in members of the Picornaviridae virus family, such as foot and mouth disease virus (FMDV), equine rhinitis A virus (ERAV0), Thosea asigna virus (TaV), and porcine teschovirus-1 (PTV-1); and cardioviruses such as tylovirus and encephalomyocarditis virus. The 2A peptides derived from FMDV, ERAV, PTV-1, and TaV are referred to herein as "F2A", "E2A", "P2A" and "T2A", respectively. One of skill in the art would be able to select an appropriate self-cleaving peptide for use in the present invention.

[0203] In some embodiments, the linker further comprises a nucleic acid sequence encoding a furin cleavage site. Furin is a ubiquitously expressed protease that resides in the trans-Golgi and processes protein precursors prior to secretion. Furin cleaves at the COOH-terminus of its consensus recognition sequence. A variety of furin consensus recognition sequences (or "furin cleavage sites") are known to those of skill in the art and include, but are not limited to, Arg-X1-Lys-Arg (SEQ ID NO:143) or Arg-X1-Arg-Arg (SEQ ID NO:144), X2-Arg-X1-X3-Arg (SEQ ID NO:145) and Arg-X1-X1-Arg (SEQ ID NO:146), such as Arg-Gln-Lys-Arg (SEQ ID NO:147), where X1 is any naturally occurring amino acid, X2 is Lys or Arg, and X3 is Lys or Arg. Those of skill in the art will be able to select a suitable furin cleavage site for use in the present invention.

[0204] In some embodiments, the linker comprises a nucleic acid sequence encoding a combination of a furin cleavage site and a 2A peptide. Examples include, but are not limited to, a linker comprising a nucleic acid sequence encoding a furin cleavage site and F2A, a linker comprising a nucleic acid sequence encoding a furin cleavage site and E2A, a linker comprising a nucleic acid sequence encoding a furin cleavage site and P2A, and a linker comprising a nucleic acid sequence encoding a furin cleavage site and T2A. Those skilled in the art will be able to select the appropriate combination for use in the present invention. In such embodiments, the linker may further comprise a spacer sequence between the furin cleavage site and the 2A peptide. In some embodiments, the linker comprises a furin cleavage site 5' of the 2A peptide. In some embodiments, the linker comprises a 2A peptide 5' of the furin cleavage site. A variety of spacer sequences are known in the art, including, but not limited to, glycine serine (GS) spacers, such as (GS), (GSGGS) (SEQ ID NO:119) and (GGGS) (SEQ ID NO:120), where n represents an integer of at least 1. Exemplary spacer sequences can include amino acid sequences including, but not limited to, GGSG (SEQ ID NO:122), GGSGG (SEQ ID NO:123), GSGSG (SEQ ID NO:124), GSGGG (SEQ ID NO:125), GGGSG (SEQ ID NO:126), GSSSG (SEQ ID NO:127), and the like. One of skill in the art will be able to select an appropriate spacer sequence for use in the present invention.

[0205] In some aspects, the nucleic acids of the disclosure may be operably linked to transcriptional regulatory elements, such as promoters and enhancers. Suitable promoter and enhancer elements are known to those of skill in the art.

[0206] In certain embodiments, the nucleic acid encoding the exogenous CAR is in operably linked to a promoter. In certain embodiments, the promoter is a phosphoglycerate kinase-1 (PGK) promoter.

[0207] Suitable promoters for expression in bacterial cells include, but are not limited to, lacI, lacZ, T3, T7, gpt, lambda P and trc. Suitable promoters for expression in eukaryotic cells include, but are not limited to, light and / or heavy chain immunoglobulin gene promoter and enhancer elements; cytomegalovirus immediate early promoter; herpes simplex virus thymidine kinase promoter; early and late SV40 promoter; promoters present in long terminal repeats from retroviruses; mouse metallothionein-I promoter; and various tissue-specific promoters known in the art. Suitable reversible promoters, including reversible inducible promoters, are known in the art. Such reversible promoters can be isolated and derived from many organisms, e.g., eukaryotes and prokaryotes. Modification of reversible promoters from a first organism for use in a second organism (e.g., a first prokaryote and a second eukaryote, a first eukaryote and a second prokaryote, etc.) is well known in the art.Such reversible promoters, and systems based on such reversible promoters but also including additional regulatory proteins, include, but are not limited to, alcohol-regulated promoters (e.g., alcohol dehydrogenase I (alcA) gene promoter, alcohol transactivator protein (A1cR) responsive promoter, etc.), tetracycline-regulated promoters, (e.g., promoter systems including TetActivator, TetON, TetOFF, etc.), steroid-regulated promoters (e.g., rat glucocorticoid receptor promoter system, human estrogen receptor promoter system, retinoid promoter system, thyroid promoter system, ecdysone promoter system, mifepristone promoter system, etc.), metal-regulated promoters (e.g., metallothionein promoter system, etc.), pathogenesis-associated regulated promoters (e.g., salicylic acid-regulated promoters, ethylene-regulated promoters, benzothiadiazole-regulated promoters, etc.), temperature-regulated promoters (e.g., heat shock-inducible promoters (e.g., HSP-70, HSP-90, soybean heat shock promoter, etc.), light-regulated promoters, synthetic inducible promoters, etc.

[0208] In some embodiments, the promoter is a CD8 cell-specific promoter, a CD4 cell-specific promoter, a neutrophil-specific promoter, or a NK cell-specific promoter.For example, the CD4 gene promoter can be used; see, for example, Salmon et al. Proc. Natl. Acad. Sci. USA (1993) 90:7739; and Marodon et al. (2003) Blood 101:3416.As another example, the CD8 gene promoter can be used.NK cell-specific expression can be achieved by using the NcrI (p46) promoter; see, for example, Eckelhart et al. Blood (2011) 117:1565.

[0209] Suitable promoters for expression in yeast cells are constitutive promoters such as the ADH1 promoter, the PGK1 promoter, the ENO promoter, the PYK1 promoter, etc.; or regulatable promoters such as the GAL1 promoter, the GAL10 promoter, the ADH2 promoter, the PHOS promoter, the CUP1 promoter, the GALT promoter, the MET25 promoter, the MET3 promoter, the CYC1 promoter, the HIS3 promoter, the ADH1 promoter, the PGK promoter, the GAPDH promoter, the ADC1 promoter, the TRP1 promoter, the URA3 promoter, the LEU2 promoter, the ENO promoter, the TP1 promoter, and the AOX1 (e.g., for use in Pichia). Selection of the appropriate vector and promoter is well within the level of one of ordinary skill in the art. Promoters suitable for use in prokaryotic host cells include, but are not limited to, the bacteriophage T7 RNA polymerase promoter; the trp promoter; the lac operon promoter; hybrid promoters, e.g., the lac / tac hybrid promoter, the tac / trc hybrid promoter, the trp / lac promoter, the T7 / lac promoter; the trc promoter; the tac promoter, and the like; the araBAD promoter; in vivo regulated promoters such as the ssaG promoter or related promoters (see, e.g., U.S. Patent Application Publication No. 20040131637), the pagC promoter (Pulkkinen and Miller, J. Bacteriol. (1991) 173(1): 86-93; Alpuche-Aranda et al., Proc. Natl. Acad. Sci. USA (1992) 89(21): 10079-83), the nirB promoter (Harborne et al. Mol. Micro. (1992) 6:2805-2813), and the like (see, for example, Dunstan et al., Infect. Immun. (1999) 67:5133-5141; McKelvie et al., Vaccine (2004) 22:3243-3255; and Chatfield et al., Biotechnol.(1992) 10:888-892); sigma70 promoters, e.g., the consensus sigma70 promoter (see, e.g., GenBank Accession Nos. AX798980, AX798961, and AX798183); stationary phase promoters, e.g., the dps promoter, the spv promoter, and the like; promoters from pathogenicity island SPI-2 (see, e.g., WO96 / 17951); actA promoters (see, e.g., Shetron-Rama et al., Infect. Immun. (2002) 70:1087-1096); rpsM promoters (see, e.g., Valdivia and Falkow Mol. Microbiol. (1996). 22:367); tet promoters (see, e.g., Hillen, W. and Wissmann, A. (1989) In Saenger, W. and Heinemann, U. (eds), Topics in Molecular and Structural Biology, Protein--Nucleic Acid Interaction. Macmillan, London, UK, Vol. 10, pp. 143-162); SP6 promoter (see, e.g., Melton et al., Nucl. Acids Res.(1984) 12:7035). Strong promoters suitable for use in prokaryotes, such as Escherichia coli, include, but are not limited to, Trc, Tac, T5, T7, and Plambda. Non-limiting examples of operators for use in bacterial host cells include the lactose promoter operator (the LacI repressor protein changes conformation when contacted with lactose, thereby preventing the Lad repressor protein from binding to the operator), the tryptophan promoter operator (when complexed with tryptophan, the TrpR repressor protein has a conformation that binds the operator; in the absence of tryptophan, the TrpR repressor protein has a conformation that does not bind to the operator), and the tac promoter operator (see, e.g., deBoer et al., Proc. Natl. Acad. Sci. USA (1983) 80:21-25).

[0210] Other examples of suitable promoters include the immediate early cytomegalovirus (CMV) promoter sequence. This promoter sequence is a strong constitutive promoter sequence capable of driving high levels of expression of any polynucleotide sequence operably linked to it. Other constitutive promoter sequences may also be used, including but not limited to the simian virus 40 (SV40) early promoter, mouse mammary tumor virus (MMTV) or human immunodeficiency virus (HIV) long terminal repeat (LTR) promoter, MoMuLV promoter, avian leukemia virus promoter, Epstein-Barr virus immediate early promoter, Rous sarcoma virus promoter, EF-1 alpha promoter, and human gene promoters such as but not limited to actin promoter, myosin promoter, hemoglobin promoter, and creatine kinase promoter. Furthermore, the present invention should not be limited to the use of constitutive promoters. Inducible promoters are also contemplated as part of the present invention. The use of an inducible promoter provides a molecular switch capable of turning on expression of the polynucleotide sequence to which it is operably linked when such expression is desired, or turning off expression when expression is not desired. Examples of inducible promoters include, but are not limited to, metallothionine promoters, glucocorticoid promoters, progesterone promoters, and tetracycline promoters.

[0211] In some embodiments, the locus or construct or transgene containing a suitable promoter is irreversibly switched via induction of an inducible system. Suitable systems for induction of irreversible switches are well known in the art, for example, induction of irreversible switches may utilize Cre-lox mediated recombination (see, for example, Fuhrmann-Benzakein, et al., Proc. Natl. Acad. Sci. USA (2000) 28:e99, the disclosure of which is incorporated herein by reference). Any suitable combination of recombinases, endonucleases, ligases, recombination sites, etc., known in the art may be used to generate irreversibly switchable promoters. Methods, mechanisms, and requirements for performing site-specific recombination described elsewhere herein are used to generate irreversibly switched promoters and are well known in the art. See, e.g., Grindley et al. Annual Review of Biochemistry (2006) 567-605; and Tropp, Molecular Biology (2012) (Jones & Bartlett Publishers, Sudbury, Mass.), the disclosures of which are incorporated herein by reference.

[0212] In some embodiments, the nucleic acid of the present disclosure further comprises a nucleic acid sequence encoding a CAR-inducing expression cassette. In one embodiment, the CAR-inducing expression cassette is for the production of a transgenic polypeptide product that is released upon CAR signaling. See, for example, Chmielewski and Abken, Expert Opin. Biol. Ther. (2015) 15(8): 1145-1154; and Abken, Immunotherapy (2015) 7(5): 535-544. In some embodiments, the nucleic acid of the present disclosure further comprises a nucleic acid sequence encoding a cytokine that is operably linked to a T cell activation response promoter. In some embodiments, the cytokine that is operably linked to a T cell activation response promoter is present on a separate nucleic acid sequence. In one embodiment, the cytokine is IL-12.

[0213] The nucleic acids of the present disclosure may be present in an expression vector and / or a cloning vector. Expression vectors may include a selection marker, an origin of replication, and other features that provide for replication and / or maintenance of the vector. Suitable expression vectors include, for example, plasmids, viral vectors, and the like. Numerous suitable vectors and promoters are known to those of skill in the art; many are commercially available for generating recombinant constructs of interest. The following vectors are provided by way of example, but should not be construed as limiting in any way: Bacteria: pBs, phagescript, PsiX174, pBluescript SK, pBs KS, pNH8a, pNH16a, pNH18a, pNH46a (Stratagene, La Jolla, Calif., USA); pTrc99A, pKK223-3, pKK233-3, pDR540, and pRIT5 (Pharmacia, Uppsala, Sweden). Prokaryotic: pWLneo, pSV2cat, pOG44, PXR1, pSG (Stratagene) pSVK3, pBPV, pMSG and pSVL (Pharmacia).

[0214] Expression vectors generally have convenient restriction sites located near the promoter sequence to provide for the insertion of nucleic acid sequences encoding heterologous proteins. A selectable marker operative in the expression host may be present. Suitable expression vectors include viral vectors (e.g., vaccinia virus; poliovirus; adenovirus (e.g., Li et al., Invest. Opthalmol. Vis. Sci. (1994) 35: 2543-2549; Borras et al., Gene Ther. (1999) 6: 515-524; Li and Davidson, Proc. Natl. Acad. Sci. USA (1995) 92: 7700-7704; Sakamoto et al., H. Gene Ther. (1999) 5: 1088-1097; WO 94 / 12649, WO 93 / 03769, WO 93 / 19191, WO 94 / 28938, WO 95 / 11984, and WO 95 / 00655); adeno-associated viruses (see, e.g., Ali et al., Hum. Gene Ther. (1998) 9: 81-86, Flannery et al., Proc. Natl. Acad. Sci. USA (1997) 94: 6916-6921; Bennett et al., Invest. Opthalmol. Vis. Sci. (1997) 38: 2857-2863; Jomary et al., Gene Ther. (1997) 4:683 690, Rolling et al., Hum. Gene Ther. (1999) 10: 641-648; Ali et al., Hum. Mol. Genet. (1996) 5: 591-594; Srivastava in WO 93 / 09239, Samulski et al., J. Vir. (1989) 63: 3822-3828; Mendelson et al., Virol. (1988) 166: 154-165; and Flotte et al., Proc. Natl. Acad. Sci.USA (1993) 90: 10613-10617); SV40; herpes simplex virus; viral vectors based on human immunodeficiency virus (see, e.g., Miyoshi et al., Proc. Natl. Acad. Sci. USA (1997) 94: 10319-23; Takahashi et al., J. Virol. (1999) 73: 7812-7816); retroviral vectors (e.g., vectors derived from murine leukemia virus, spleen necrosis virus, and retroviruses such as Rous sarcoma virus, Harvey sarcoma virus, avian leukosis virus, human immunodeficiency virus, myeloproliferative sarcoma virus, and mammary tumor virus).

[0215] Additional expression vectors suitable for use include, but are not limited to, lentivirus vectors, gamma retrovirus vectors, foamy virus vectors, adeno-associated virus vectors, adenovirus vectors, poxvirus vectors, herpes virus vectors, engineered hybrid virus vectors, transposon-mediated vectors, etc. Viral vector techniques are well known in the art and are described, for example, in Sambrook et al., 2012, Molecular Cloning: A Laboratory Manual, volumes 1-4, Cold Spring Harbor Press, NY) and other virology and molecular biology manuals. Viruses useful as vectors include, but are not limited to, retroviruses, adenoviruses, adeno-associated viruses, herpes viruses, and lentiviruses.

[0216] Generally, suitable vectors contain an origin of replication functional in at least one organism, a promoter sequence, convenient restriction endonuclease sites, and one or more selectable markers (e.g., WO 01 / 96584; WO 01 / 29058; and U.S. Pat. No. 6,326,193).

[0217] In some embodiments, an expression vector (e.g., lentiviral vector) may be used to introduce a CAR into immune cells or their precursor cells (e.g., T cells). Thus, an expression vector (e.g., lentiviral vector) of the present invention may comprise a nucleic acid encoding a CAR. In some embodiments, an expression vector (e.g., lentiviral vector) will comprise additional elements that aid in the functional expression of the CAR encoded therein. In some embodiments, an expression vector comprising a nucleic acid encoding a CAR further comprises a mammalian promoter. In one embodiment, the vector further comprises an elongation factor-1-alpha promoter (EF-1α promoter). Use of the EF-1α promoter may increase the efficiency in the expression of downstream transgenes (e.g., nucleic acid sequences encoding a CAR). Physiological promoters (e.g., EF-1α promoters) may be less likely to induce integration-mediated genotoxicity and may negate the ability of retroviral vectors to transform stem cells. Other physiological promoters suitable for use in vectors (e.g., lentiviral vectors) are known to those skilled in the art and may be incorporated into the vectors of the present invention. In some embodiments, the vector (e.g., lentiviral vector) further comprises non-essential cis-acting sequences that may improve titer and gene expression. A non-limiting example of a non-essential cis-acting sequence is the central polypurine tract and central termination sequence (cPPT / CTS), which is important for efficient reverse transcription and nuclear transport. Other non-essential cis-acting sequences are known to those skilled in the art and may be incorporated into the vector (e.g., lentiviral vector) of the present invention. In some embodiments, the vector further comprises a post-transcriptional regulatory element. The post-transcriptional regulatory element may improve the translation of RNA, improve the expression of the transgene, and stabilize the RNA transcript. One example of a post-transcriptional regulatory element is the Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element (WPRE). Thus, in some embodiments, the vector for the present invention further comprises a WPRE sequence. A variety of post-transcriptional regulator elements are known to those skilled in the art and may be incorporated into the vector (e.g., lentiviral vector) of the present invention.The vector of the present invention may further comprise additional elements such as a rev response element (RRE) for transport of RNA, a packaging sequence, and 5' and 3' long terminal repeats (LTRs). The term "long terminal repeats" or "LTRs" refers to a domain of base pairs located at the end of retroviral DNA, including the U3, R and U5 regions. LTRs generally provide functions necessary for retroviral gene expression (e.g., promotion, initiation and polyadenylation of gene transcripts) and viral replication. In one embodiment, the vector of the present invention (e.g., lentiviral vector) comprises a 3' U3 deleted LTR. Thus, the vector of the present invention (e.g., lentiviral vector) may comprise any combination of elements described herein to increase the efficiency of functional expression of the transgene. For example, the vector of the present invention (e.g., lentiviral vector) may comprise a WPRE sequence, a cPPT sequence, an RRE sequence, a 5'LTR, a 3' U3 deleted LTR' in addition to the nucleic acid encoding CAR.

[0218] The vector of the present invention can be a self-inactivating vector. The term "self-inactivating vector" as used herein refers to a vector in which the 3' LTR enhancer promoter region (U3 region) is modified (e.g., by deletion or substitution). A self-inactivating vector can prevent viral transcription beyond the first round of viral replication. As a result, a self-inactivating vector can infect and then be integrated into the host genome (e.g., mammalian genome) only once, but cannot be further passaged. Therefore, a self-inactivating vector can greatly reduce the risk of generating replication-competent virus.

[0219] In some embodiments, the nucleic acid of the present invention can be RNA, for example, in vitro synthesized RNA. Methods for in vitro synthesis of RNA are known to those skilled in the art; any known method can be used to synthesize RNA comprising a sequence encoding a CAR of the present disclosure. Methods for introducing RNA into a host cell are known in the art. See, for example, Zhao et al. Cancer Res. (2010) 15: 9053. Introducing RNA comprising a nucleotide sequence encoding a CAR of the present disclosure into a host cell can be performed in vitro or ex vivo or in vivo. For example, host cells (e.g., NK cells, cytotoxic T lymphocytes, etc.) can be electroporated with RNA comprising a nucleotide sequence encoding a CAR of the present disclosure in vitro or ex vivo.

[0220] To evaluate the expression of a polypeptide or a portion thereof, the expression vector to be introduced into cells may also contain one or both of a selection marker gene or a reporter gene to facilitate the identification and selection of expressing cells from the cell population to be transfected or infected by the viral vector.In some embodiments, the selection marker may be carried on a separate piece of DNA and used in a co-transfection procedure.Both the selection marker and the reporter gene may be adjacent to the appropriate regulatory sequence to allow expression in the host cell.Useful selection markers include, but are not limited to, antibiotic resistance genes.

[0221] Reporter genes are used to identify potentially transfected cells and to evaluate the functionality of regulatory sequences. In general, reporter genes are genes that are not present in or expressed by recipient organisms or tissues, and code for polypeptides whose expression is manifested by some easily detectable properties, such as enzymatic activity. The expression of reporter genes is evaluated at a suitable time after DNA is introduced into recipient cells. Suitable reporter genes may include, but are not limited to, luciferase, beta-galactosidase, chloramphenicol acetyltransferase, genes that code for secreted alkaline phosphatase, or green fluorescent protein genes (e.g., Ui-Tei et al., 2000 FEBS Letters 479: 79-82).

[0222] In some aspects, the nucleic acids of the disclosure provide for the production of a CAR as described herein, e.g., in a mammalian cell. In some aspects, the nucleic acids of the disclosure provide for the amplification of the nucleic acid encoding the CAR.

[0223] D. Engineered Immune Cells The present invention provides modified immune cells or precursors thereof (e.g., T cells) that contain a chimeric antigen receptor (CAR) capable of binding to PSCA (e.g., human PSCA). Also provided are modified immune cells or precursors thereof that contain bispecific CARs (e.g., PSCA&PSMA), PSCA CARs with dominant negative receptors (e.g., TGFbRDN), PSCA CARs with switch receptors (e.g., PD1 / CD28 or TGFbR / IL12R), and PSCA CARs in combination with bispecific antibodies (e.g., PD-L1 / CD28). The present invention also includes modified immune cells or precursors thereof that contain any of the nucleic acids disclosed herein or any of the vectors disclosed herein.

[0224] In one aspect, the invention includes an engineered immune cell, or a precursor thereof, that comprises a CAR that comprises an antigen-binding domain capable of binding to PSCA, a transmembrane domain, and an intracellular domain.

[0225] In certain exemplary aspects, the antigen binding domain comprises three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6).

[0226] In another aspect, the disclosure provides an engineered immune cell or progenitor thereof comprising a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), the CAR comprising an antigen binding domain, a transmembrane domain, and an intracellular domain, wherein the antigen binding domain comprises a heavy chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and a light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8.

[0227] In another aspect, the present disclosure provides an engineered immune cell, or a precursor thereof, comprising a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), the chimeric antigen receptor (CAR) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:9.

[0228] In certain exemplary embodiments, the transmembrane domain comprises the transmembrane domain of CD8 alpha.

[0229] In certain exemplary embodiments, the intracellular domain comprises a costimulatory signaling domain and an intracellular signaling domain. In certain exemplary embodiments, the costimulatory signaling domain comprises the costimulatory domain of 4-1BB. In certain exemplary embodiments, the costimulatory signaling domain comprises the costimulatory domain of CD28. In certain exemplary embodiments, the costimulatory signaling domain comprises the costimulatory domain of ICOS. In certain exemplary embodiments, the costimulatory signaling domain comprises the costimulatory domain of ICOS (YMNM). In certain exemplary embodiments, the intracellular signaling domain comprises the intracellular domain of CD3zeta or a variant thereof.

[0230] In another aspect, the disclosure provides an engineered immune cell, or a precursor thereof, comprising a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA), the chimeric antigen receptor (CAR) comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:21, 23, 25 or 27.

[0231] In certain exemplary embodiments, the modified cells further comprise a PSMA-CAR, which comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain.

[0232] In one aspect, the invention includes an engineered immune cell, or a precursor thereof, that comprises a bispecific chimeric antigen receptor (CAR) that comprises an extracellular domain that comprises an antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen binding domain capable of binding to prostate specific membrane antigen (PSMA).

[0233] In certain embodiments, the extracellular domain of the bispecific CAR comprises an antigen binding domain capable of binding to PSCA comprising: a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The extracellular domain also comprises an antigen-binding domain capable of binding to PSMA, comprising: a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0234] In certain embodiments, the extracellular domain comprises an antigen binding domain capable of binding to PSCA comprising: a first heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; a first light chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or a second heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34; and a second light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:35, and an antigen-binding domain capable of binding to PSMA.

[0235] In certain embodiments, the extracellular domain of the bispecific CAR comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). and / or an antigen binding domain capable of binding to PSMA comprising a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence EYTIH (SEQ ID NO:68), HCDR2 comprises the amino acid sequence NINPNNGGTTYNQKFED (SEQ ID NO:69), and HCDR3 comprises the amino acid sequence GWNFDY (SEQ ID NO:70); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence KASQDVGTAVD (SEQ ID NO:71), LCDR2 comprises the amino acid sequence WASTRHT (SEQ ID NO:72), and LCDR3 comprises the amino acid sequence QQYNSYPLT (SEQ ID NO:73).

[0236] In certain embodiments, the extracellular domain of the bispecific CAR comprises an antigen-binding domain capable of binding to PSCA, comprising: a first heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; a first light chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or a second heavy chain variable region comprising an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75; and a second light chain variable region comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:77, and an antigen-binding domain capable of binding to PSMA.

[0237] In certain exemplary embodiments, the bispecific CAR can bind to human PSCA. In certain exemplary embodiments, the bispecific CAR can bind to human PSMA. In certain exemplary embodiments, the bispecific CAR can bind to human PSCA and human PSMA.

[0238] In another aspect, the invention includes an engineered immune cell, or a precursor thereof, comprising a first CAR comprising a first antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and a second CAR comprising a second antigen binding domain capable of binding to prostate specific membrane antigen (PSMA), wherein the first and second CAR each comprise a transmembrane domain and an intracellular domain.

[0239] In certain exemplary aspects, the first antigen binding domain comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The second antigen-binding domain comprises a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence SNWIG (SEQ ID NO:28), HCDR2 comprises the amino acid sequence IIYPGDSDTRYSPSFQG (SEQ ID NO:29), and HCDR3 comprises the amino acid sequence QTGFLWSFDL (SEQ ID NO:30); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence RASQDISSALA (SEQ ID NO:31), LCDR2 comprises the amino acid sequence DASSLES (SEQ ID NO:32), and LCDR3 comprises the amino acid sequence QQFNSYPLT (SEQ ID NO:33).

[0240] In certain exemplary embodiments, the first heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and / or the first light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or the second heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:34; and / or the second light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:35. It comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:35.

[0241] In certain exemplary aspects, the first antigen binding domain comprises a first heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence DYYIH (SEQ ID NO:1), HCDR2 comprises the amino acid sequence WIDPENGDTEFVPKFQG (SEQ ID NO:2), and HCDR3 comprises the amino acid sequence TGGF (SEQ ID NO:3); and a first light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence SASSSVRFIHW (SEQ ID NO:4), LCDR2 comprises the amino acid sequence DTSKLAS (SEQ ID NO:5), and LCDR3 comprises the amino acid sequence QQWSSSPFT (SEQ ID NO:6). The second antigen-binding domain comprises a second heavy chain variable region comprising three heavy chain complementarity determining regions (HCDRs), where HCDR1 comprises the amino acid sequence EYTIH (SEQ ID NO:68), HCDR2 comprises the amino acid sequence NINPNNGGTTYNQKFED (SEQ ID NO:69), and HCDR3 comprises the amino acid sequence GWNFDY (SEQ ID NO:70); and a second light chain variable region comprising three light chain complementarity determining regions (LCDRs), where LCDR1 comprises the amino acid sequence KASQDVGTAVD (SEQ ID NO:71), LCDR2 comprises the amino acid sequence WASTRHT (SEQ ID NO:72), and LCDR3 comprises the amino acid sequence QQYNSYPLT (SEQ ID NO:73).

[0242] In certain exemplary embodiments, the first heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:7; and / or the first light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:8; and / or the second heavy chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75; and / or the second light chain variable region comprises an amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:75. It comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to NO:77.

[0243] In certain exemplary embodiments, the first CAR and the second CAR each comprise an artificial hydrophobic sequence and a transmembrane domain selected from the group consisting of a transmembrane domain of a type I transmembrane protein, the alpha, beta or zeta chain of the T cell receptor, CD28, CD3 epsilon, CD45, CD4, CD5, CD8, CD9, CD16, CD22, CD33, CD37, CD64, CD80, CD86, OX40 (CD134), 4-1BB (CD137), ICOS, and CD154, or a transmembrane domain derived from a killer immunoglobulin-like receptor (KIR). In certain exemplary embodiments, the first CAR and the second CAR each comprise a transmembrane domain of CD8 alpha.

[0244] In certain exemplary embodiments, the intracellular domain of the first CAR and the second CAR each comprises a costimulatory signaling domain and an intracellular signaling domain.

[0245] In certain exemplary embodiments, the intracellular domain of the first CAR and the second CAR each comprises a costimulatory domain of a protein selected from the group consisting of proteins in the TNFR superfamily, CD28, 4-1BB (CD137), OX40 (CD134), PD-1, CD7, LIGHT, CD83L, DAP10, DAP12, CD27, CD2, CD5, ICAM-1, LFA-1, Lck, TNFR-I, TNFR-II, Fas, CD30, CD40, ICOS, NKG2C, and B7-H3 (CD276), or variants thereof, or an intracellular domain derived from a killer immunoglobulin-like receptor (KIR). In certain exemplary embodiments, the intracellular domain of the first CAR and the second CAR each comprises a costimulatory domain of 4-1BB.

[0246] In certain exemplary embodiments, the intracellular signaling domain of the first CAR and the second CAR each comprises an intracellular domain selected from the group consisting of the cytoplasmic signaling domain of human CD3 zeta chain (CD3ζ), FcγRIII, FcsRI, the cytoplasmic tail of an Fc receptor, an immunoreceptor tyrosine-based activation motif (ITAM) bearing cytoplasmic receptor, TCR zeta, FcR gamma, CD3 gamma, CD3 delta, CD3 epsilon, CD5, CD22, CD79a, CD79b, and CD66d, or variants thereof. In certain exemplary embodiments, the intracellular signaling domain of the first CAR and the second CAR each comprises the intracellular domain of CD3ζ, or variants thereof.

[0247] In certain exemplary embodiments, the first CAR comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:21, and / or the second CAR comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:67, 87, 89, 91, 93, 95 or 97.

[0248] In certain embodiments, the modified cell further comprises a dominant negative receptor. In certain embodiments, the dominant negative receptor is a truncated variant of the wild-type protein associated with negative signaling. In certain exemplary embodiments, the truncated variant of the wild-type protein associated with negative signaling comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:56.

[0249] In certain embodiments, the modified cell further comprises a switch receptor. In certain embodiments, the switch receptor comprises a first domain from a first switch polypeptide associated with a negative signal; and a second domain from a second switch polypeptide associated with a positive signal. In certain exemplary embodiments, the switch receptor comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:58, 60 or 62.

[0250] Thus, in certain aspects, the invention includes modified immune cells or precursor cells thereof comprising a CAR comprising an antigen binding domain capable of binding to PSCA, a transmembrane domain, and an intracellular domain, the immune cells or precursor cells thereof further comprising a dominant negative receptor (e.g., TGFbRDN).

[0251] Thus, in certain aspects, the invention includes an engineered immune cell, or a precursor thereof, comprising a CAR comprising an antigen binding domain capable of binding to PSCA, a transmembrane domain, and an intracellular domain, the immune cell, or a precursor thereof, further comprising a switch receptor (e.g., a PD1 / CD28 switch receptor).

[0252] Also provided is an engineered immune cell, or a precursor thereof, comprising a CAR capable of binding to prostate stem cell antigen (PSCA-CAR) and a bispecific antibody, wherein the CAR comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain, and the engineered immune cell, or a precursor thereof, secretes the bispecific antibody.

[0253] In certain embodiments, the modified cell further comprises a bispecific antibody, and the cell secretes the bispecific antibody. In certain embodiments, the bispecific antibody comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% identical to SEQ ID NO:64 or 66.

[0254] In certain embodiments, the modified cell is a modified immune cell. In certain embodiments, the modified cell is a modified T cell. In certain embodiments, the modified cell is an autologous cell. In certain embodiments, the modified cell is an autologous cell obtained from a human subject.

[0255] E. Sources of Immune Cells In some embodiments, the source of immune cells (e.g., T cells) for ex vivo manipulation is obtained from a subject. The source of immune cells for ex vivo manipulation can also include, for example, autologous or heterologous donor blood, umbilical cord blood, or bone marrow. For example, the source of immune cells can be derived from the subject to be treated by the modified immune cells of the present invention, and can be, for example, the subject's blood, the subject's umbilical cord blood, or the subject's bone marrow. Non-limiting examples of subjects include humans, dogs, cats, mice, rats, and their transgenic species. Preferably, the subject is a human.

[0256] Immune cells can be obtained from several sources, including blood, peripheral blood mononuclear cells, bone marrow, lymph node tissue, spleen tissue, umbilical cord, lymph, or lymphoid organs. Immune cells are cells of the immune system, such as cells of innate or adaptive immunity, such as lymphocytes, typically myeloid or lymphoid cells, including T cells and / or NK cells. Other exemplary cells include stem cells, such as multipotent and multipotent stem cells, including induced pluripotent stem cells (iPSCs). In some aspects, the cells are human cells. With respect to the subject to be treated, the cells can be allogeneic and / or autologous. The cells are typically primary cells, such as primary cells that are directly isolated from the subject and / or isolated and frozen from the subject.

[0257] In certain embodiments, the immune cell is a T cell, e.g., a CD8+ T cell (e.g., a CD8+ naive T cell, a central memory T cell, or an effector memory T cell), a CD4+ T cell, a natural killer T cell (NKT cell), a regulatory T cell (Treg), a stem cell memory T cell, a lymphoid progenitor cell, a hematopoietic stem cell, a natural killer cell (NK cell), or a dendritic cell. In some embodiments, the cell is a monocyte or a granulocyte, e.g., a myeloid cell, a macrophage, a neutrophil, a dendritic cell, a mast cell, an eosinophil, and / or a basophil. In one embodiment, the target cell is an induced pluripotent stem (iPS) cell or a cell derived from an iPS cell, e.g., an iPS cell that has been generated from a subject and engineered to alter (e.g., induce mutations in) or manipulate expression of one or more target genes, e.g., differentiated into a T cell, e.g., a CD8+ T cell (e.g., a CD8+ naive T cell, a central memory T cell, or an effector memory T cell), a CD4+ T cell, a stem cell memory T cell, a lymphoid progenitor cell, or a hematopoietic stem cell.

[0258] In some embodiments, the cells include one or more subsets of T cells or other cell types, such as the entire T cell population, CD4+ cells, CD8+ cells, and subpopulations thereof, such as those defined by function, activation state, maturation, differentiation potential, expansion, recirculation, localization, and / or persistence capacity, antigen specificity, antigen receptor type, presence in a particular organ or compartment, marker or cytokine secretion profile, and / or degree of differentiation. Among the subtypes and subpopulations of T cells and / or CD4+ and / or CD8+ T cells are naive T (TN) cells, effector T cells (TEFF), memory T cells and their subtypes, such as stem cell memory T (TSCM), central memory T (TCM), effector memory T (TEM), or terminally differentiated effector memory T cells, tumor infiltrating lymphocytes (TIL), immature T cells, mature T cells, helper T cells, cytotoxic T cells, mucosal-associated invariant T (MAIT) cells, innate and adaptive regulatory T (Treg) cells, helper T cells, such as TH1 cells, TH2 cells, TH3 cells, TH17 cells, TH9 cells, TH22 cells, follicular helper T cells, alpha / beta T cells, and delta / gamma T cells. In certain embodiments, any number of T cell lines available in the art may be used.

[0259] In some embodiments, the method includes steps of isolating immune cells from a subject, preparing, treating, culturing, and / or manipulating them. In some embodiments, the preparation of the engineered cells includes one or more culturing and / or preparation steps. The cells for manipulation as described may be isolated from a sample, such as a biological sample, for example a sample obtained or derived from a subject. In some embodiments, the subject from which the cells are isolated is a subject with a disease or condition, or a subject in need of or to whom cell therapy will be administered. The subject is, in some embodiments, a human in need of a particular therapeutic intervention, such as adoptive cell therapy, for which the cells are isolated, treated, and / or manipulated. Thus, the cells are, in some embodiments, primary cells, for example, primary human cells. Samples include tissues, fluids, and other samples taken directly from a subject, as well as samples resulting from one or more processing steps, such as separation, centrifugation, genetic manipulation (e.g., transduction with a viral vector), washing, and / or incubation. The biological sample can be a sample obtained directly from a biological source or a processed sample. Biological samples include, but are not limited to, body fluids such as blood, plasma, serum, cerebrospinal fluid, synovial fluid, urine and sweat, tissues, and organ samples, including processed samples derived therefrom.

[0260] In some aspects, the sample from which the cells are derived or isolated is a blood or blood-derived sample, or is or is derived from an apheresis or leukapheresis product.Exemplary samples include whole blood, peripheral blood mononuclear cells (PBMCs), white blood cells, bone marrow, thymus, tissue biopsy, tumor, leukemia, lymphoma, lymph node, gut-associated lymphoid tissue, mucosa-associated lymphoid tissue, spleen, other lymphoid tissue, liver, lung, stomach, intestine, colon, kidney, pancreas, breast, bone, prostate, cervix, testis, ovary, tonsil, or other organ, and / or cells derived therefrom.Samples include samples from autologous and allogeneic sources in the context of cell therapy, e.g., adoptive cell therapy.

[0261] In some embodiments, the cells are derived from a cell line, e.g., a T cell line. In some embodiments, the cells are obtained from a heterologous source, e.g., mouse, rat, non-human primate, and pig. In some embodiments, the isolation of cells includes one or more preparation steps and / or cell separation steps that are not based on affinity. In some examples, the cells are washed, centrifuged, and / or incubated in the presence of one or more reagents, e.g., to remove undesired components, enrich for desired components, and lyse or remove cells that are sensitive to a particular reagent. In some examples, the cells are separated based on one or more properties, such as density, adhesive properties, size, sensitivity and / or resistance to a particular component.

[0262] In some examples, cells from the subject's circulating blood are obtained, for example, by apheresis or leukapheresis. The sample contains lymphocytes, including T cells, monocytes, granulocytes, B cells, other nucleated white blood cells, red blood cells, and / or platelets, and in some aspects cells other than red blood cells and platelets. In some embodiments, blood cells collected from a subject are washed, for example to remove the plasma fraction and place the cells in a buffer or medium suitable for the subsequent processing step. In some embodiments, the cells are washed with phosphate buffered saline (PBS). In some aspects, the washing step is accomplished by tangential flow filtration (TFF) according to the manufacturer's instructions. In some embodiments, the cells are resuspended in various biocompatible buffers after washing. In certain embodiments, components of the blood cell sample are removed and the cells are resuspended directly in the medium. In some embodiments, the method includes a density-based cell separation method, such as preparation of white blood cells from peripheral blood by lysing red blood cells and centrifuging through a Percoll or Ficoll gradient.

[0263] In one embodiment, the immune cells are cells obtained from the circulating blood of an individual obtained by apheresis or leukapheresis. Apheresis products typically contain lymphocytes, including T cells, monocytes, granulocytes, B cells, other nucleated white blood cells, red blood cells, and platelets. Cells collected by apheresis may be washed to remove the plasma fraction and place the cells in a suitable buffer or medium, such as phosphate-buffered saline (PBS), or the washing solution may be calcium-free and magnesium-free, or may be free of many, if not all, divalent cations, for subsequent processing steps. After washing, the cells may be resuspended in a variety of biocompatible buffers, such as Ca-free, Mg-free PBS, etc. Alternatively, undesirable components of the apheresis sample may be removed and the cells resuspended directly in culture medium.

[0264] In some embodiments, the isolation method comprises the separation of different cell types based on the expression or presence in cells of one or more specific molecules, such as surface markers, e.g., surface proteins, intracellular markers, or nucleic acids.In some embodiments, any known method may be used for such marker-based separation.In some embodiments, the separation is affinity or immunoaffinity-based separation.For example, in some aspects, the separation comprises the separation of cells and cell populations based on the cellular expression or expression level of one or more markers, typically cell surface markers, for example, by incubation with an antibody or binding partner that specifically binds to such marker, generally followed by a washing step, and separation of the cells that bind to the antibody or binding partner from the cells that do not bind to the antibody or binding partner.

[0265] Such separation steps can be based on positive selection, where cells bound to the reagent are retained for further use, and / or negative selection, where cells that are not bound to the antibody or binding partner are retained. In some instances, both fractions are retained for further use. In some aspects, negative selection can be particularly useful when antibodies that specifically identify cell types in a heterogeneous population are not available, such that separation is best performed based on markers expressed by cells other than the desired population. Separation does not need to result in 100% enrichment or removal of a particular cell population or cells expressing a particular marker. For example, positive selection or enrichment of a particular type of cell, such as cells expressing a marker, refers to increasing the number or percentage of such cells, but does not need to result in the complete absence of cells that do not express the marker. Similarly, negative selection, removal, or depletion of a particular type of cell, such as cells expressing a marker, refers to decreasing the number or percentage of such cells, but does not need to result in the complete removal of all such cells.

[0266] In some examples, multiple rounds of separation steps are performed, with the positively or negatively selected fraction from one step being subjected to another separation step, such as a subsequent positive or negative selection. In some examples, cells expressing multiple markers simultaneously can be depleted in a single separation step, such as by incubating cells with multiple antibodies or binding partners, each specific to the marker targeted for negative selection. Similarly, multiple cell types can be positively selected simultaneously by incubating cells with multiple antibodies or binding partners expressed on various cell types.

[0267] In some embodiments, one or more of the T cell populations are positive for (marker+) or express high levels of one or more particular markers, e.g., surface markers. high ) cells, or cells that are negative for one or more markers (marker -) or expresses it at a relatively low level (marker low ) cells are enriched or depleted. For example, in some aspects, a particular subpopulation of T cells, e.g., cells positive for or expressing high levels of one or more surface markers, e.g., CD28+, CD62L+, CCR7+, CD27+, CD127+, CD4+, CD8+, CD45RA+, and / or CD45RO+ T cells, are isolated by positive or negative selection techniques. In some cases, such markers are markers that are absent or expressed at relatively low levels on certain populations of T cells (e.g., non-memory cells) but present or expressed at relatively high levels on certain other populations of T cells (e.g., memory cells). In one embodiment, the cells (CD8+ cells, or T cells, such as CD3+ cells) are enriched for cells that are positive for or express high surface levels of CD45RO, CCR7, CD28, CD27, CD44, CD127, and / or CD62L (i.e., positively selected for), and / or are depleted for cells that are positive for or express high surface levels of CD45RA (e.g., negatively selected for). In some embodiments, the cells are enriched for or depleted for cells that are positive for or express high surface levels of CD122, CD95, CD25, CD27, and / or IL7-Ra (CD127). In some examples, the CD8+ T cells are enriched for cells that are positive for CD45RO (or negative for CD45RA) and positive for CD62L. For example, CD3+,CD28+ T cells can be positively selected using CD3 / CD28 conjugated magnetic beads (e.g., DYNABEADS® M-450 CD3 / CD28 T Cell Expander).

[0268] In some embodiments, T cells are separated from the PBMC sample by negative selection of markers expressed on non-T cells such as B cells, monocytes, or other white blood cells, e.g., CD14. In some aspects, a CD4+ or CD8+ selection step is used to separate CD4+ helper T cells and CD8+ cytotoxic T cells. Such CD4+ and CD8+ populations can be further sorted into subpopulations by positive or negative selection for markers expressed or expressed to a relatively high degree on one or more naive, memory, and / or effector T cell subpopulations. In some embodiments, CD8+ cells are further enriched or depleted for naive, central memory, effector memory, and / or central memory stem cells, e.g., by positive or negative selection based on surface antigens associated with each subpopulation. In some embodiments, enrichment for central memory T (TCM) cells is performed to increase efficacy, e.g., to improve long-term survival, expansion, and / or engraftment following administration, which in some aspects is particularly robust in such subpopulations. In some embodiments, combining TCM-enriched CD8+ T cells and CD4+ T cells further enhances efficacy.

[0269] In some embodiments, memory T cells are present in both CD62L+ and CD62L- subsets of CD8+ peripheral blood lymphocytes. PBMCs can be enriched or depleted for CD62L-CD8+ and / or CD62L+CD8+ fractions using anti-CD8 and anti-CD62L antibodies, etc. In some embodiments, CD4+ T cell population and CD8+ T cell subpopulation, such as a subpopulation enriched for central memory (TCM) cells. In some embodiments, enrichment for central memory T (TCM) cells is based on positive or high surface expression of CD45RO, CD62L, CCR7, CD28, CD3, and / or CD127; in some aspects, it is based on negative selection for cells expressing or highly expressing CD45RA and / or granzyme B. In some aspects, the isolation of a CD8+ population enriched for TCM cells is performed by depletion of cells expressing CD4, CD14, CD45RA, and positive selection or enrichment for cells expressing CD62L. In one aspect, the enrichment of central memory T (TCM) cells is performed starting from a negative fraction of cells selected based on CD4 expression, which is subjected to negative selection based on CD14 and CD45RA expression, and positive selection based on CD62L. Such selections are performed simultaneously in some aspects, and sequentially in either order in other aspects. In some aspects, the same selection step based on CD4 expression used to prepare a CD8+ cell population or subpopulation is also used to generate a CD4+ cell population or subpopulation, whereby both the positive and negative fractions from CD4-based separation are retained and used in subsequent steps of the method, optionally following one or more additional positive or negative selection steps.

[0270] CD4+ T helper cells are sorted into naive, central memory, and effector cells by identifying cell populations with cell surface antigens. CD4+ lymphocytes can be obtained by standard methods. In some embodiments, naive CD4+ T lymphocytes are CD45RO-, CD45RA+, CD62L+, CD4+ T cells. In some embodiments, central memory CD4+ cells are CD62L+ and CD45RO+. In some embodiments, effector CD4+ cells are CD62L- and CD45RO. In one example, to enrich for CD4+ cells by negative selection, the monoclonal antibody cocktail typically includes antibodies against CD14, CD20, CD11b, CD16, HLA-DR, and CD8. In some embodiments, the antibodies or binding partners are bound to a solid support or matrix, such as magnetic or paramagnetic beads, to allow for the separation of cells for positive and / or negative selection.

[0271] In some embodiments, the cells are incubated and / or cultured prior to or in conjunction with genetic engineering. The incubation step can include culture, cultivation, stimulation, activation, and / or propagation. In some embodiments, the composition or cells are incubated in the presence of stimulatory conditions or stimuli. Such conditions include conditions designed to induce proliferation, expansion, activation, and / or survival of cells in a population, to mimic antigen exposure, and / or to prime cells for genetic engineering, such as introduction of a recombinant antigen receptor. Conditions can include one or more of a particular medium, temperature, oxygen content, carbon dioxide content, time, agents, such as nutrients, amino acids, antibiotics, ions, and / or stimulatory factors, such as cytokines, chemokines, antigens, binding partners, fusion proteins, recombinant soluble receptors, and any other agents designed to activate cells. In some embodiments, the stimulatory conditions or stimuli include one or more agents, such as ligands, capable of activating the intracellular signaling domain of the TCR complex. In some aspects, the agent initiates or initiates the TCR / CD3 intracellular signaling cascade in T cells. Such an agent can include, for example, an antibody bound to a solid support such as a bead, for example, an antibody specific for a TCR component and / or a costimulatory receptor, for example, anti-CD3, anti-CD28, and / or one or more cytokines. Optionally, the expansion method can further include adding anti-CD3 and / or anti-CD28 antibodies to the culture medium (e.g., at a concentration of at least about 0.5 ng / ml). In some embodiments, the stimulator includes IL-2 and / or IL-15, for example, an IL-2 concentration of at least about 10 units / mL.

[0272] In another embodiment, T cells are isolated from peripheral blood by lysing red blood cells and depleting monocytes, for example, by centrifugation through a PERCOLL™ gradient. Alternatively, T cells can be isolated from umbilical cord. In any event, specific subpopulations of T cells can be further isolated by positive or negative selection techniques.

[0273] The cord blood mononuclear cells so isolated can be depleted of cells expressing certain antigens, including but not limited to CD34, CD8, CD14, CD19, and CD56. Depletion of these cells can be accomplished using isolated antibodies, biological samples containing antibodies, such as ascites fluid, antibodies bound to a physical support, or antibodies bound to cells.

[0274] Enrichment of a T cell population by negative selection can be accomplished using a combination of antibodies against surface markers unique to the negatively selected cells. A preferred method is cell sorting and / or selection via negative magnetic immunoadherence or flow cytometry using a cocktail of monoclonal antibodies against cell surface markers present on the negatively selected cells. For example, negative selection can be used to enrich for CD4 + To enrich for cells, the monoclonal antibody cocktail typically includes antibodies against CD14, CD20, CD11b, CD16, HLA-DR, and CD8.

[0275] To isolate the desired population of cells by positive or negative selection, cell concentration and surface (e.g., particles such as beads) can be varied. In certain embodiments, it may be desirable to significantly reduce the volume in which beads and cells are mixed together (i.e., increase the concentration of cells) to ensure maximum contact between cells and beads. For example, in one embodiment, a cell concentration of 2 billion cells / ml is used. In one embodiment, a cell concentration of 1 billion cells / ml is used. In a further embodiment, more than 100 million cells / ml is used. In a further embodiment, a cell concentration of 10, 15, 20, 25, 30, 35, 40, 45, or 50 million cells / ml is used. In yet another embodiment, a cell concentration of 75, 80, 85, 90, 95, or 100 million cells / ml is used. In further embodiments, cell concentrations of 125 or 150 million / ml can be used. Using higher concentrations can result in increased cell yield, cell activation, and cell expansion.

[0276] T cells can also be frozen after a washing step that does not require a monocyte removal step. Without wishing to be bound by theory, the freezing and subsequent thawing steps provide a more uniform product by removing granulocytes and to some extent monocytes in the cell population. After a washing step that removes plasma and platelets, the cells may be suspended in a freezing solution. Although numerous freezing solutions and parameters are known in the art and would be useful in this context, in a non-limiting example, one method involves using PBS containing 20% ​​DMSO and 8% human serum albumin, or other suitable cell freezing medium. The cells are then frozen to -80°C at a rate of 1°C / min and stored in the vapor phase of a liquid nitrogen storage tank. Other methods of controlled freezing and immediate uncontrolled freezing at -20°C or in liquid nitrogen may also be used.

[0277] In one embodiment, the T cells are comprised in a population of cells such as peripheral blood mononuclear cells, umbilical cord blood cells, purified populations of T cells, and T cell lines. In another embodiment, the peripheral blood mononuclear cells comprise the T cell population. In yet another embodiment, the purified T cells comprise the T cell population.

[0278] In certain embodiments, regulatory T cells (Tregs) can be isolated from a sample. The sample can include, but is not limited to, umbilical cord blood or peripheral blood. In certain embodiments, Tregs are isolated by flow cytometry sorting. The sample can be enriched for Tregs before isolation by any means known in the art. The isolated Tregs can be cryopreserved and / or expanded before use. Methods for isolating Tregs are described in U.S. Patent Nos. 7,754,482, 8,722,400, and 9,555,105, and U.S. Patent Application No. 13 / 639,927, the contents of which are incorporated herein in their entirety.

[0279] F. Treatment Method The modified immune cells (e.g., T cells) described herein can be included in a composition for immunotherapy. The composition can include a pharmaceutical composition and can further include a pharma- ceutically acceptable carrier. A therapeutically effective amount of the pharmaceutical composition comprising the modified T cells can be administered.

[0280] In one aspect, the invention includes a method of treating a disease or condition in a subject, comprising administering to a subject in need thereof an effective amount of a modified cell (e.g., T cell) of the invention. In another aspect, the invention includes a method of treating a disease or condition in a subject, comprising administering to a subject in need thereof a pharmaceutical composition comprising an effective amount of a modified cell (e.g., T cell) of the invention. In another aspect, the invention includes a method for adoptive cell transfer therapy, comprising administering to a subject in need thereof an effective amount of a modified cell (e.g., T cell) of the invention.

[0281] Methods for administration of immune cells for adoptive cell therapy are known and may be used with the provided methods and compositions.For example, adoptive T cell therapy is described in, for example, Gruenberg et al., U.S. Patent Application Publication No. 2003 / 0170238; Rosenberg, U.S. Patent No. 4,690,915; Rosenberg (2011) Nat Rev Clin Oncol. 8(10):577-85).See, for example, Themeli et al. (2013) Nat Biotechnol. 31(10): 928-933; Tsukahara et al. (2013) Biochem Biophys Res Commun 438(1): 84-9; Davila et al. (2013) PLoS ONE 8(4): e61338. In some embodiments, cell therapy, e.g., adoptive T cell therapy, is carried out by autologous transfer, where cells are isolated and / or otherwise prepared from the subject who will receive cell therapy or a sample derived from such a subject.Thus, in some aspects, cells are derived from a subject, e.g., a patient, in need of treatment, and the cells are administered to the same subject after isolation and processing.

[0282] In some embodiments, cell therapy, such as adoptive T cell therapy, is carried out by allogeneic transfer, in which cells are isolated and / or otherwise prepared from a subject other than the subject that will or will eventually receive cell therapy, such as the first subject.In such embodiments, the cells are then administered to a different subject of the same species, such as the second subject.In some embodiments, the first and second subjects are genetically identical.In some embodiments, the first and second subjects are genetically similar.In some embodiments, the second subject expresses the same HLA class or supertype as the first subject.

[0283] In some embodiments, the subject has been treated with a disease or condition, e.g., a tumor-targeting therapeutic agent, prior to administration of the cell or cell-containing composition. In some aspects, the subject is refractory or non-responsive to other therapeutic agents. In some embodiments, the subject has persistent or recurrent disease after treatment with another therapeutic intervention, e.g., chemotherapy, radiation, and / or hematopoietic stem cell transplantation (HSCT), e.g., allogeneic HSCT. In some embodiments, administration effectively treats the subject even though the subject has become resistant to another therapy.

[0284] In some embodiments, the subject is responsive to another therapeutic agent, and treatment with the therapeutic agent reduces the disease burden. In some aspects, the subject is initially responsive to the therapeutic agent, but exhibits recurrence of the disease or condition over time. In some embodiments, the subject has not relapsed. In some such embodiments, the subject is determined to be at risk of relapse, e.g., at high risk of relapse, and thus the cells are administered prophylactically, e.g., to reduce the likelihood of relapse or prevent relapse. In some aspects, the subject has not received prior treatment with another therapeutic agent.

[0285] In some embodiments, the subject has persistent or recurrent disease after treatment with another therapeutic intervention, including, for example, chemotherapy, radiation, and / or hematopoietic stem cell transplantation (HSCT), e.g., allogeneic HSCT. In some embodiments, administration effectively treats the subject despite the subject having become refractory to another therapy.

[0286] The modified immune cells of the present invention can be administered to animals, preferably mammals, and even more preferably humans, to treat cancer. In addition, the cells of the present invention can be used for the treatment of any condition related to cancer, particularly cell-mediated immune response against tumor cells, when it is desirable to treat or alleviate the disease. Types of cancer to be treated with the modified cells or pharmaceutical compositions of the present invention include carcinomas, blastomas, and sarcomas, as well as certain leukemias or lymphoid tumors, benign and malignant tumors, and malignant diseases, such as sarcomas, carcinomas, and melanomas. Other exemplary cancers include, but are not limited to, breast cancer, prostate cancer, ovarian cancer, cervical cancer, skin cancer, pancreatic cancer, colorectal cancer, kidney cancer, liver cancer, brain cancer, lymphoma, leukemia, lung cancer, thyroid cancer, and the like. The cancer can be a non-solid tumor (such as a blood tumor) or a solid tumor. Adult tumors / cancers and pediatric tumors / cancers are also included. In one embodiment, the cancer is a solid tumor or a hematological tumor. In one embodiment, the cancer is a type of carcinoma. In one embodiment, the cancer is a sarcoma. In one aspect, the cancer is leukemia. In one aspect, the cancer is a solid tumor.

[0287] A solid tumor is an abnormal mass of tissue that does not usually contain cysts or liquid areas. Solid tumors can be benign or malignant. Different types of solid tumors are named for the type of cells that form them (such as sarcoma, carcinoma, and lymphoma). Examples of solid tumors, such as sarcoma and carcinoma, include fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteosarcoma, and other sarcomas, synovium, mesothelioma, Ewing's sarcoma, leiomyosarcoma, rhabdomyosarcoma, colon cancer, lymphatic tumors, pancreatic cancer, breast cancer, lung cancer, ovarian cancer, prostate cancer, hepatocellular carcinoma, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, medullary thyroid carcinoma, papillary thyroid carcinoma, pheochromocytoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, and sebaceous gland carcinoma. In some embodiments, the cancer is a glioma. In some embodiments, the cancer is a high-grade astrocytoma. In some embodiments, the cancer is a prostate cancer. In some embodiments, the cancer is a prostate cancer. In some embodiments, the cancer is a prostate cancer. In some embodiments, the cancer is a prostate cancer. In some embodiments, the cancer is a prostate cancer. In some embodiments, the cancer is a prostate cancer.

[0288] Carcinomas amenable to treatment by the methods disclosed herein include, but are not limited to, esophageal carcinoma, hepatocellular carcinoma, basal cell carcinoma (a form of skin cancer), squamous cell carcinoma (various tissues), bladder carcinoma including transitional cell carcinoma (malignant neoplasm of the bladder), bronchogenic carcinoma, colon carcinoma, colorectal carcinoma, gastric carcinoma, lung carcinoma including small cell lung carcinoma and non-small cell lung carcinoma, adrenal cortical carcinoma, thyroid carcinoma, pancreatic carcinoma, breast cancer, ovarian cancer, prostate cancer, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinoma, cystadenocarcinoma, medullary carcinoma, renal cell carcinoma, ductal carcinoma in situ or bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, cervical carcinoma, uterine carcinoma, testicular carcinoma, osteogenic carcinoma, epithelial carcinoma, and nasopharyngeal carcinoma.

[0289] Sarcomas that may be treated by the methods disclosed herein include, but are not limited to, fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, chordoma, osteogenic sarcoma, osteosarcoma, angiosarcoma, endothelial sarcoma, lymphangiosarcoma, lymphangioendothelial sarcoma, synovium, mesothelioma, Ewing's sarcoma, leiomyosarcoma, rhabdomyosarcoma, and other soft tissue sarcomas.

[0290] In certain exemplary embodiments, the modified immune cells of the present invention are used to treat myeloma or a condition associated with myeloma. Examples of myeloma or a condition associated with myeloma include, but are not limited to, light chain myeloma, non-secretory myeloma, monoclonal gammopathy of undetermined significance (MGUS), plasmacytoma (e.g., solitary plasmacytoma, multiple solitary plasmacytoma, extramedullary plasmacytoma), amyloidosis, and multiple myeloma. In one embodiment, the method of the present disclosure is used to treat multiple myeloma. In one embodiment, the method of the present disclosure is used to treat refractory myeloma. In one embodiment, the method of the present disclosure is used to treat relapsed myeloma.

[0291] In certain exemplary aspects, the modified immune cells of the present invention are used to treat melanoma or melanoma-related conditions. Examples of melanoma or melanoma-related conditions include, but are not limited to, superficial spreading melanoma, nodular melanoma, lentigo maligna melanoma, acral lentigo melanoma, amelanotic melanoma, or cutaneous melanoma (e.g., cutaneous melanoma, ocular melanoma, vulvar melanoma, vaginal melanoma, rectal melanoma). In one aspect, the method of the present disclosure is used to treat cutaneous melanoma. In one aspect, the method of the present disclosure is used to treat refractory melanoma. In one aspect, the method of the present disclosure is used to treat recurrent melanoma.

[0292] In yet another exemplary embodiment, the modified immune cells of the present invention are used to treat sarcomas or sarcoma-related conditions. Examples of sarcomas or sarcoma-related conditions include, but are not limited to, angiosarcoma, chondrosarcoma, Ewing's sarcoma, fibrosarcoma, gastrointestinal stromal tumor, leiomyosarcoma, liposarcoma, malignant peripheral nerve sheath tumor, osteosarcoma, pleomorphic sarcoma, rhabdomyosarcoma, and synovial sarcoma. In one embodiment, the method of the present disclosure is used to treat synovial sarcoma. In one embodiment, the method of the present disclosure is used to treat liposarcoma, such as myxoid / round cell liposarcoma, differentiated / dedifferentiated liposarcoma, and pleomorphic liposarcoma. In one embodiment, the method of the present disclosure is used to treat myxoid / round cell liposarcoma. In one embodiment, the method of the present disclosure is used to treat refractory sarcomas. In one embodiment, the method of the present disclosure is used to treat recurrent sarcomas.

[0293] The cells of the invention to be administered can be autologous to the subject undergoing treatment.

[0294] Administration of the cells of the present invention may be performed in any convenient manner known to those skilled in the art. The cells of the present invention may be administered to a subject by aerosol inhalation, injection, ingestion, transfusion, implantation or transplantation. The compositions described herein may be administered to a patient intraarterially, subcutaneously, intradermally, intratumorally, intranodally, intramedullary, intramuscularly, by intravenous (iv) injection, or intraperitoneally. In other examples, the cells of the present invention are directly injected into a site of inflammation in a subject, a site of local disease in a subject, a lymph node, an organ, a tumor, etc.

[0295] In some embodiments, the cells are administered at a desired dosage, which in some aspects includes a desired dose or number of cells or cell types and / or a desired ratio of cell types. Thus, the dosage of cells is based in some embodiments on the total number of cells (or number per kg body weight) and the desired ratio of individual populations or subtypes, such as the ratio of CD4+ to CD8+. In some embodiments, the dosage of cells is based on the desired total number of cells (or number per kg body weight) in an individual population or of an individual cell type. In some embodiments, the dosage is based on a combination of such features, such as the desired total number of cells, the desired ratio, and the desired total number of cells in an individual population.

[0296] In some embodiments, CD8 + T cells and CD4 + A population or subtype of cells, such as T cells, is administered at or within an acceptable variance of a desired dose of total cells, e.g., a desired dose of T cells. In some aspects, the desired dose is a desired number of cells, or a desired number of cells per unit body weight of the subject to whom the cells are administered, e.g., cells / kg. In some aspects, the desired dose is at or exceeds a minimum number of cells or a minimum number of cells per unit body weight. In some aspects, among the total cells administered at a desired dose, individual populations or subtypes are administered at or above a desired output ratio (CD4 + vs. CD8 + (e.g., a ratio of 0.1 to 0.5), e.g., within a certain allowed variation or error of such ratio.

[0297] In some embodiments, the cells are administered at or within a tolerable difference of the desired dose of one or more of the individual populations or subtypes of cells, e.g., the desired dose of CD4+ cells and / or the desired dose of CD8+ cells. In some aspects, the desired dose is the desired number of cells of a subtype or population, or the desired number of such cells per unit body weight of the subject to whom the cells are administered, e.g., cells / kg. In some aspects, the desired dose is or exceeds the minimum number of cells of a population or subtype, or the minimum number of cells of a population or subtype per unit body weight. Thus, in some embodiments, the dosage is based on a desired fixed dose and a desired ratio of total cells, and / or based on one or more, e.g., each, of the individual subtypes or subpopulations. Thus, in some embodiments, the dosage is based on a desired fixed dose or minimum dose of T cells and CD4 + Cells vs. CD8 + Based on the desired ratio of cells and / or CD4 + Cells and / or CD8 + Based on a desired fixed or minimum dose of cells.

[0298] In certain embodiments, a distinct population of cells, or subtypes of cells, is administered to a subject in the range of about 1 million to about 100 billion cells, e.g., 1 million to about 50 billion cells (e.g., about 5 million cells, about 25 million cells, about 500 million cells, about 1 billion cells, about 5 billion cells, about 20 billion cells, about 30 billion cells, about 40 billion cells, or a range defined by any two of the foregoing values), e.g., about 10 million to about 100 billion cells (e.g., about 20 million cells, about 30 million cells, about 40 million cells, about 60 million cells, about 70 million cells, or a range defined by any two of the foregoing values). , about 80 million cells, about 90 million cells, about 10 billion cells, about 25 billion cells, about 50 billion cells, about 75 billion cells, about 90 billion cells, or a range defined by any two of the foregoing values), and optionally about 100 million cells to about 50 billion cells (e.g., about 120 million cells, about 250 million cells, about 350 million cells, about 450 million cells, about 650 million cells, about 800 million cells, about 900 million cells, about 3 billion cells, about 30 billion cells, about 45 billion cells), or any value between these ranges.

[0299] In some embodiments, the dose of total cells and / or the dose of individual subpopulations of cells is greater than or equal to 1×10 cells. 5 1 x 10 pieces or approx. 5 pieces / kg ~ approx. 1×10 11 cells / kg, 10 cells 4 ~10 pieces 11 Pieces or about 10 11 pieces / kilogram (kg) body weight, e.g., 10 cells 5 ~10 6 In the range of 1 x 10 cells / kg body weight, for example, 5 pcs or approx. 1×10 5 cells / kg, cells 1.5×10 5 cells / kg, cells 2×10 5 cells / kg, or 1 x 10 cells 6 For example, in some embodiments, the cells are 10 T cells / kg body weight. 4 Pieces or about 10 4 ~10 pieces 9 Pieces or about 109 cells / kilogram (kg) body weight, e.g., 10 T cells 5 ~10 pieces 6 / kg body weight or within a certain margin of error, e.g., 1 x 10 T cells 5 pcs or approx. 1×10 5 cells / kg, T cells 1.5×10 5 cells / kg, T cells 2×10 5 cells / kg, or 1 x 10 T cells 6 In other exemplary embodiments, the dosage range of modified cells suitable for use in the methods of the present disclosure is about 1×10 cells / kg body weight. 5 cells / kg~cells approx. 1×10 6 cells / kg, approximately 1×10 cells 6 cells / kg~cells approx. 1×10 7 cells / kg, approximately 1×10 cells 7 cells / kg~cells approx. 1×10 8 cells / kg, approximately 1×10 cells 8 cells / kg~cells approx. 1×10 9 cells / kg, approximately 1×10 cells 9 cells / kg~cells approx. 1×10 10 cells / kg, approximately 1×10 cells 10 cells / kg~cells approx. 1×10 11 In an exemplary embodiment, a dosage suitable for use in the methods of the present disclosure is about 1×10 cells / kg. 8 In an exemplary embodiment, a dosage suitable for use in the methods of the present disclosure is about 1×10 cells / kg. 7 In other embodiments, a suitable dosage is about 1×10 total cells / kg. 7 ~ Total cells: approx. 5 x 10 7 In some embodiments, a suitable dosage is about 1×10 total cells. 8 ~ Total cells: approx. 5 x 10 8 In some embodiments, a suitable dosage is about 1.4 x 10 total cells. 7 Total cells: approx. 1.1 x 10 9 In an exemplary embodiment, a suitable dosage for use in the methods of the present disclosure is about 7×10 total cells. 9 There are 10.

[0300] In some embodiments, the cells are 4 or about 10 4 ~10 9 or about 10 9 CD4 count / kilogram (kg) of body weight + and / or CD8 + Cells, e.g., 10 5 ~10 6 CD4 count / kg body weight + and / or CD8 + in cells, or within a certain margin of error, e.g., 1 x 10 5 Or about 1 x 10 5 CD4 cells / kg + and / or CD8 + cells, 1.5 x 10 5 CD4 cells / kg + and / or CD8 + cells, 2 x 10 5 CD4 cells / kg + and / or CD8 + cells, or 1 x 10 6 CD4 count / kg body weight + and / or CD8 + In some embodiments, the cells are administered at a concentration of about 1×10 6 pieces, approximately 2.5×10 6 pieces, about 5×10 6 pieces, approximately 7.5×10 6 Pieces, or about 9 x 10 6 More than 1×10 6 pieces, approximately 2.5×10 6 pieces, about 5×10 6 pieces, approximately 7.5×10 6 Pieces, or about 9 x 10 6 CD4 + cells, and / or at least about 1 x 10 6 pieces, approximately 2.5×10 6 pieces, about 5×10 6 pieces, approximately 7.5×10 6 Pieces, or about 9 x 10 6 CD8 + cells, and / or at least about 1 x 10 6 pieces, approximately 2.5×106 pieces, about 5×10 6 pieces, approximately 7.5×10 6 Pieces, or about 9 x 10 6 In some embodiments, the cells are administered at about 10 T cells or within a certain margin of error. 8 ~10 pieces 12 Pieces or about 10 10 ~10 pieces 11 T cells, approximately 10 8 ~10 pieces 12 Pieces or about 10 10 ~10 pieces 11 CD4 + cells, and / or about 10 8 ~10 pieces 12 Pieces or about 10 10 ~10 pieces 11 CD8 + The dose is administered at or within a certain range of error in cells.

[0301] In some embodiments, the cells are administered at a desired output ratio or within a tolerated range of multiple cell populations or subtypes, such as CD4+ and CD8+ cells or subtypes. In some aspects, the desired ratio can be a specific ratio or can be a range of ratios, e.g., in some embodiments, the desired ratio (e.g., CD4 + Cells vs. CD8 +The ratio of cells is 5:1 or about 5:1 to 5:1 or about 5:1 (or greater than about 1:5 and less than about 5:1), or 1:3 or about 1:3 to 3:1 or about 3:1 (or greater than about 1:3 and less than about 3:1), for example, 2:1 or about 2:1 to 1:5 or about 1:5 (or greater than about 1:5 and less than about 2:1, for example, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.9:1, 1.8:1, 1.7:1, 1.6:1, 1.5:1, 1.4:1, 1.3:1, 1.2:1, 1.1:1, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5 , or 1:5, or about 5:1, about 4.5:1, about 4:1, about 3.5:1, about 3:1, about 2.5:1, about 2:1, about 1.9:1, about 1.8:1, about 1.7:1, about 1.6:1, about 1.5:1, about 1.4:1, about 1.3:1, about 1.2:1, about 1.1:1, about 1:1, about 1:1.1, about 1:1.2, about 1:1.3, about 1:1.4, about 1:1.5, about 1:1.6, about 1:1.7, about 1:1.8, about 1:1.9, about 1:2, about 1:2.5, about 1:3, about 1:3.5, about 1:4, about 1:4.5, or about 1:5. In some aspects, tolerated differences are within about 1%, about 2%, about 3%, about 4%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50% of the desired ratio, including any value between these ranges.

[0302] In some embodiments, the dose of modified cells is administered to the subject in need thereof in a single dose or multiple doses.In some embodiments, the dose of modified cells is administered in multiple doses, for example, once a week or every 7 days, once every 2 weeks or every 14 days, once every 3 weeks or every 21 days, once every 4 weeks or every 28 days.In exemplary embodiments, a single dose of modified cells is administered to the subject in need thereof.In exemplary embodiments, a single dose of modified cells is administered to the subject in need thereof by rapid intravenous infusion.

[0303] The dosage appropriate for preventing or treating a disease may depend on the type of disease being treated, the type of cells or recombinant receptor, the severity and course of the disease, whether the cells are administered for prophylactic or therapeutic purposes, prior therapy, the subject's clinical history and response to the cells, and the judgment of the attending physician. The compositions and cells are, in some embodiments, administered to a subject at one time or over a series of treatments, as appropriate.

[0304] In some embodiments, the cells are administered as part of a combination treatment, e.g., simultaneously with another therapeutic intervention, e.g., an antibody or engineered cell or receptor, or an agent, e.g., a cytotoxic or therapeutic agent, or sequentially in any order. The cells are co-administered in some embodiments simultaneously or sequentially in any order with one or more additional therapeutic agents or in association with another therapeutic intervention. In some situations, the cells are co-administered with another therapy close enough in time that the cell population enhances the effect of the one or more additional therapeutic agents, or vice versa. In some embodiments, the cells are administered before the one or more additional therapeutic agents. In some embodiments, the cells are administered after the one or more additional therapeutic agents. In some embodiments, the one or more additional agents include a cytokine, such as IL-2, for example, to enhance persistence. In some embodiments, the method includes administration of a chemotherapeutic agent.

[0305] In certain embodiments, the modified cells of the present invention (e.g., modified cells comprising a CAR) may be administered to a subject in combination with an inhibitor of an immune checkpoint. Examples of immune checkpoints include, but are not limited to, CTLA-4, PD-1, and TIM-3. An antibody may be used to inhibit an immune checkpoint (e.g., an anti-PD1 antibody, an anti-CTLA-4 antibody, or an anti-TIM-3 antibody). For example, the modified cells may be administered in combination with, for example, an antibody or antibody fragment that targets PD-1 (programmed death 1 protein). Examples of anti-PD-1 antibodies include, but are not limited to, pembrolizumab (KEYTRUDA®, formerly known as lambrolizumab, MK-3475), and nivolumab (BMS-936558, MDX-1106, ONO-4538, OPDIVA®), or an antigen-binding fragment thereof. In certain embodiments, the modified cells may be administered in combination with an anti-PD-L1 antibody, or an antigen-binding fragment thereof. Examples of anti-PD-L1 antibodies include, but are not limited to, BMS-936559, MPDL3280A (TECENTRIQ®, atezolizumab), and MEDI4736 (durvalumab, Imfinzi). In certain embodiments, the modified cells may be administered in combination with an anti-CTLA-4 antibody or an antigen-binding fragment thereof. An example of an anti-CTLA-4 antibody includes, but is not limited to, ipilimumab (trade name Yervoy). Other types of immune checkpoint modulators may be used, including but not limited to small molecules, siRNA, miRNA, and CRISPR systems. The immune checkpoint modulator may be administered before, after, or simultaneously with the modified cells comprising the CAR. In certain embodiments, combination treatments including immune checkpoint modulators may enhance the therapeutic efficacy of therapies including the modified cells of the present invention.

[0306] Following administration of the cells, in some embodiments, the biological activity of the engineered cell population is measured, for example, by any of a number of known methods. Parameters for evaluation include specific binding of engineered or natural T cells or other immune cells to antigens, for example, in vivo by imaging, or ex vivo, for example, by ELISA or flow cytometry. In certain embodiments, the ability of the engineered cells to destroy target cells can be measured using any suitable method known in the art, such as, for example, cytotoxicity assays described in Kochenderfer et al., J. Immunotherapy, 32(7): 689-702 (2009), and Herman et al. J. Immunological Methods, 285(1): 25-40 (2004). In certain embodiments, the biological activity of the cells is measured by assaying the expression and / or secretion of one or more cytokines, such as CD 107a, IFNγ, IL-2, and TNF. In some aspects, the biological activity is measured by evaluating clinical outcomes, such as reduction in tumor burden or tumor burden.

[0307] In certain embodiments, the subject is provided with a secondary treatment, including, but not limited to, chemotherapy, radiation, surgery, and drug therapy.

[0308] In some embodiments, conditioning therapy can be administered to the subject prior to CAR T cell therapy. In some embodiments, conditioning therapy comprises administering an effective amount of cyclophosphamide to the subject. In some embodiments, conditioning therapy comprises administering an effective amount of fludarabine to the subject. In a preferred embodiment, conditioning therapy comprises administering an effective amount of a combination of cyclophosphamide and fludarabine to the subject. Administering conditioning therapy prior to CAR T cell therapy can enhance the efficacy of CAR T cell therapy. Methods for conditioning patients for T cell therapy are described in U.S. Patent No. 9,855,298, which is incorporated herein by reference in its entirety.

[0309] In some embodiments, certain dosing regimens of the present disclosure include a lymphodepletion step prior to administration of the modified T cells. In exemplary embodiments, the lymphodepletion step includes administration of cyclophosphamide and / or fludarabine.

[0310] In some embodiments, the lymphodepletion step comprises administering about 200 mg / m 2 / day~about 2000mg / m 2 / day (e.g., 200 mg / m 2 / day, 300mg / m 2 / day or 500 mg / m 2 / day). In an exemplary embodiment, the dose of cyclophosphamide is about 300 mg / m 2 In some embodiments, the lymphodepletion step is at about 20 mg / m 2 / day~about 900mg / m 2 / day (e.g., 20 mg / m 2 / day, 25mg / m 2 / day, 30mg / m 2 / day or 60 mg / m 2 / day). In an exemplary embodiment, the dose of fludarabine is about 30 mg / m 2 / day.

[0311] In some embodiments, the lymphodepletion step comprises administering about 200 mg / m 2 / day~about 2000mg / m 2 / day (e.g., 200 mg / m 2 / day, 300mg / m 2 / day or 500 mg / m 2 / day) and cyclophosphamide at a dose of approximately 20 mg / m 2 / day~about 900mg / m 2 / day (e.g., 20 mg / m 2 / day, 25mg / m 2 / day, 30mg / m 2 / day or 60 mg / m 2 / day). In an exemplary embodiment, the lymphodepletion step comprises administration of fludarabine at a dose of about 300 mg / m 2 / day dose of cyclophosphamide and approximately 30 mg / m 2 The treatment involves administration of fludarabine at a dose of 100 mg / day.

[0312] In an exemplary embodiment, the dosing of cyclophosphamide is 300 mg / m for 3 days. 2 / day and fludarabine dosing was 30 mg / m for 3 days. 2 / day.

[0313] Dosing of lymphodepleting chemotherapy may be scheduled for days -6 to -4 (with a -1 day window, i.e., dosing on days -7 to -5) relative to T cell (e.g., CAR-T, TCR-T, modified T cells, etc.) infusion on day 0.

[0314] In an exemplary embodiment, for subjects with cancer, the subject is administered 300 mg / m 2 of erythropoietin by intravenous infusion 3 days prior to administration of the engineered T cells. 2 In an exemplary embodiment, for subjects with cancer, the subject receives lymphodepleting chemotherapy including cyclophosphamide at 300 mg / m by intravenous infusion for 3 days prior to administration of the modified T cells. 2 will undergo lymphodepleting chemotherapy, including cyclophosphamide.

[0315] In an exemplary embodiment, for a subject with cancer, the subject receives about 20 mg / m 2 / day~about 900mg / m 2 / day (e.g., 20 mg / m 2 / day, 25mg / m 2 / day, 30mg / m 2 / day or 60 mg / m 2 / day). In an exemplary embodiment, for a subject with cancer, the subject receives lymphodepleting chemotherapy including fludarabine at a dose of 30 mg / m 2 Patients will receive lymphodepleting chemotherapy containing a dose of fludarabine for three days.

[0316] In an exemplary embodiment, for a subject with cancer, the subject receives about 200 mg / m 2 / day~about 2000mg / m 2 / day (e.g., 200 mg / m 2 / day, 300mg / m 2 / day or 500 mg / m 2 / day) and cyclophosphamide at a dose of approximately 20 mg / m 2 / day~about 900mg / m 2 / day (e.g., 20 mg / m 2 / day, 25mg / m 2 / day, 30mg / m 2 / day or 60 mg / m 2 / day). In an exemplary embodiment, for a subject with cancer, the subject receives lymphodepleting chemotherapy including fludarabine at a dose of about 300 mg / m 2 / day dose of cyclophosphamide and 30 mg / m 2 Patients will receive lymphodepleting chemotherapy containing a dose of fludarabine for three days.

[0317] The cells of the present invention can be administered at dosages and routes and times to be determined in appropriate preclinical and clinical experiments and tests. The cell composition can be administered multiple times at dosages within these ranges. The administration of the cells of the present invention can be combined with other methods useful for treating the desired disease or condition as determined by those skilled in the art.

[0318] It is known in the art that one of the adverse effects following infusion of CAR T cells is the onset of immune activation known as cytokine release syndrome (CRS). CRS is immune activation leading to elevated inflammatory cytokines. CRS is a known on-target toxicity and its occurrence is likely to correlate with efficacy. Clinical and laboratory measures range from mild CRS (systemic symptoms and / or grade 2 organ toxicity) to severe CRS (sCRS; grade ≧3 organ toxicity, aggressive clinical intervention and / or potentially life-threatening). Clinical features include high fever, malaise, fatigue, myalgia, nausea, anorexia, tachycardia / hypotension, capillary leak, cardiac dysfunction, renal dysfunction, liver failure, and disseminated intravascular coagulation. Dramatic elevation of cytokines including interferon-gamma, granulocyte-macrophage colony-stimulating factor, IL-10, and IL-6 has been shown following CAR T cell infusion. One sign of CRS is elevated cytokines including IL-6 (severely elevated), IFN-gamma, TNF-alpha (moderately elevated), and IL-2 (mildly elevated). Elevations of clinically available markers of inflammation including ferritin and C-reactive protein (CRP) have also been observed to correlate with CRS syndrome. The presence of CRS generally correlates with expansion of adoptively transferred cells and progressive immune activation. It has been demonstrated that the severity of CRS is determined by the disease burden at the time of infusion, as patients with high tumor burden experience greater sCRS.

[0319] Therefore, the present invention provides a CRS management strategy suitable for alleviating the physiological symptoms of uncontrolled inflammation after the diagnosis of CRS without compromising the anti-tumor efficacy of engineered cells (e.g., CAR T cells).CRS management strategies are known in the art.For example, systemic corticosteroids may be administered to rapidly reverse the symptoms of sCRS (e.g., grade 3 CRS) without compromising the initial anti-tumor response.

[0320] In some embodiments, anti-IL-6R antibody may be administered.An example of anti-IL-6R antibody is the monoclonal antibody tocilizumab, also known as atlizumab, approved by the US Food and Drug Administration (commercially available as Actemra or RoActemra).Tocilizumab is a humanized monoclonal antibody against interleukin-6 receptor (IL-6R).Administration of tocilizumab has demonstrated almost immediate regression of CRS.

[0321] CRS is generally managed based on the severity of the syndrome observed, and interventions are individualized accordingly. Decisions for management of CRS can be based on clinical signs and symptoms, not just laboratory values, and response to interventions.

[0322] Mild to moderate cases are generally treated with symptom management using fluid therapy, nonsteroidal anti-inflammatory drugs (NSAIDs) and antihistamines as needed for adequate relief of symptoms. More severe cases include patients with any degree of hemodynamic instability; in any hemodynamic instability, administration of tocilizumab is recommended. First-line management of CRS may be tocilizumab IV over 60 minutes at a labelled dose of 8 mg / kg (not to exceed 800 mg / dose) in some embodiments; tocilizumab may be repeated Q8 hours. If the response to the first dose of tocilizumab is suboptimal, additional doses of tocilizumab may be considered. Tocilizumab may be administered alone or in combination with corticosteroid therapy. Patients with persistent or progressive CRS symptoms with inadequate clinical improvement or poor response to tocilizumab within 12-18 hours may be treated with high-dose corticosteroid therapy, typically hydrocortisone 100 mg IV or methylprednisolone 1-2 mg / kg. In patients with more severe hemodynamic instability or more severe respiratory symptoms, patients may be administered high-dose corticosteroid therapy early in the course of CRS. Guidance for CRS management may be based on published criteria (Lee et al. (2019) Biol Blood Marrow Transplant, doi.org / 10.1016 / j.bbmt.2018.12.758; Neelapu et al. (2018) Nat Rev Clin Oncology, 15:47; Teachey et al. (2016) Cancer Discov, 6(6):664-679).

[0323] Concurrent with the clinical signs of CRS, features consistent with macrophage activation syndrome (MAS) or hemophagocytic lymphohistiocytosis (HLH) have been observed in patients treated with CAR-T therapy (Henter, 2007). MAS appears to be a response to immune activation resulting from CRS and should therefore be considered a manifestation of CRS. MAS is similar to HLH, also a response to immune stimulation. The clinical syndrome of MAS is characterized by high-grade non-releasing fever, cytopenias affecting at least two of the three lineages, and hepatosplenomegaly. It is associated with high serum ferritin, soluble interleukin-2 receptor, and triglycerides, as well as reduced circulating natural killer (NK) activity.

[0324] The modified immune cells comprising the CAR of the present invention can be used in the method of treatment as described herein.In one aspect, the present invention comprises a method of treating cancer in a subject in need thereof, comprising administering to the subject any one of the modified immune cells or progenitor cells disclosed herein.Yet another aspect of the present invention comprises a method of treating cancer in a subject in need thereof, comprising administering to the subject any one of the modified immune cells or progenitor cells produced by any one of the methods disclosed herein.

[0325] One aspect of the present invention provides a method of treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of modified T cells comprising a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR).

[0326] In another aspect, the present invention provides a method of treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of modified T cells comprising a first chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second chimeric antigen receptor (CAR) capable of binding to a prostate specific membrane antigen (PSMA-CAR), wherein the first CAR and the second CAR each comprise an antigen binding domain, a transmembrane domain, and an intracellular domain.

[0327] Another aspect of the present invention provides a method of treating prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of an engineered T cell comprising a bispecific chimeric antigen receptor (CAR) comprising an extracellular domain, a transmembrane domain, and an intracellular domain, the extracellular domain comprising an antigen binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen binding domain capable of binding to prostate specific membrane antigen (PSMA).

[0328] Another aspect of the invention provides a method of treating prostate cancer in a subject in need thereof comprising administering to the subject an effective amount of any of the modified T cells contemplated herein.

[0329] Yet another aspect includes a method of treating metastatic castration-resistant prostate cancer in a subject in need thereof comprising administering to the subject an effective amount of modified T cells comprising a first chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a second chimeric antigen receptor (CAR) capable of binding to a prostate specific membrane antigen (PSMA-CAR), wherein the first CAR and the second CAR each comprise an antigen binding domain, a transmembrane domain, and an intracellular domain.

[0330] Also provided is a method of treating metastatic castration-resistant prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of an engineered T cell comprising a bispecific chimeric antigen receptor (CAR) comprising an extracellular domain, a transmembrane domain, and an intracellular domain, the extracellular domain comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA) and an antigen-binding domain capable of binding to prostate specific membrane antigen (PSMA).

[0331] In another aspect, the present invention provides a method of treating metastatic castration-resistant prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of modified T cells comprising a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a dominant negative receptor.

[0332] In another aspect, the present invention provides a method of treating metastatic castration-resistant prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of an engineered T cell comprising a chimeric antigen receptor (CAR) capable of binding to a prostate stem cell antigen (PSCA-CAR) and a switch receptor.

[0333] In another aspect, the present invention provides a method of treating metastatic castration-resistant prostate cancer in a subject in need thereof, comprising administering to the subject an effective amount of engineered T cells comprising a chimeric antigen receptor (CAR) capable of binding to prostate stem cell antigen (PSCA-CAR) and a bispecific antibody, wherein the engineered T cells secrete the bispecific antibody.

[0334] G. Increase in immune cells Either before or after the cells have been modified to express a CAR, the cells can be activated and expanded in number using methods such as those described in, for example, U.S. Patent Nos. 6,352,694; 6,534,055; 6,905,680; 6,692,964; 5,858,358; 6,887,466; 6,905,681; 7,144,575; 7,067,318; 7,172,869; 7,232,566; 7,175,843; 5,883,223; 6,905,874; 6,797,514; 6,867,041; and U.S. Patent Application Publication No. 20060121005. For example, the T cells of the present invention can be expanded by contacting with a surface to which an agent that stimulates CD3 / TCR complex-associated signals and a ligand that stimulates costimulatory molecules on the surface of T cells are attached.In particular, the T cell population can be stimulated by contacting with anti-CD3 antibody or its antigen-binding fragment, or anti-CD2 antibody immobilized on the surface, or by contacting with a protein kinase C activator (e.g., bryostatin) together with calcium ionophore.For costimulation of accessory molecules on the surface of T cells, a ligand that binds to the accessory molecule is used.For example, T cells can be contacted with anti-CD3 antibody and anti-CD28 antibody under conditions suitable for stimulating the proliferation of T cells. Examples of anti-CD28 antibodies include 9.3, B-T3, XR-CD28 (Diaclone, Besancon, France), which can be used in the present invention, although other methods and reagents known in the art can be used as well (see, e.g., ten Berge et al., Transplant Proc. (1998) 30(8): 3975-3977; Haanen et al., J. Exp. Med. (1999) 190(9): 1319-1328; and Garland et al., J. Immunol. Methods (1999) 227(1-2): 53-63).

[0335] The expansion of T cells by the methods disclosed herein can be about 10-fold, 20-fold, 30-fold, 40-fold, 50-fold, 60-fold, 70-fold, 80-fold, 90-fold, 100-fold, 200-fold, 300-fold, 400-fold, 500-fold, 600-fold, 700-fold, 800-fold, 900-fold, 1000-fold, 2000-fold, 3000-fold, 4000-fold, 5000-fold, 6000-fold, 7000-fold, 8000-fold, 9000-fold, 10,000-fold, 100,000-fold, 1,000,000-fold, 10,000,000-fold or more, and any and all whole integers and partial integers therebetween. In one embodiment, T cells are expanded in the range of about 20-fold to about 50-fold.

[0336] Following culture, the T cells can be incubated in the cell medium in the culture device for a period of time or until the cells reach confluence or a high cell density for optimal passaging before passaging the cells to another culture device. The culture device can be any culture device commonly used for in vitro culture of cells. Preferably, the level of confluence is 70% or more before passaging the cells to another culture device. More preferably, the level of confluence is 90% or more. The period of time can be any time suitable for in vitro cell culture. The T cell medium can be replaced at any time during the culture of the T cells. Preferably, the T cell medium is replaced about every 2-3 days. The T cells are then harvested from the culture device, after which the T cells can be used immediately or cryopreserved and stored for later use. In one aspect, the invention includes cryopreserving the expanded T cells. The cryopreserved T cells are thawed before introducing the nucleic acid into the T cells.

[0337] In another embodiment, the method comprises isolating T cells and expanding the T cells. In another embodiment, the invention further comprises cryopreserving the T cells prior to expansion. In yet another embodiment, the cryopreserved T cells are thawed for electroporation with RNA encoding the chimeric membrane protein.

[0338] Another procedure for ex vivo expansion of cells is described in U.S. Patent No. 5,199,942 (incorporated herein by reference). Expansion as described in U.S. Patent No. 5,199,942 can be an alternative or an addition to other expansion methods described herein. Briefly, ex vivo culture and expansion of T cells includes the addition of cell growth factors or other factors such as those described in U.S. Patent No. 5,199,942, such as flt3-L, IL-1, IL-3 and c-kit ligand. In one embodiment, expanding T cells includes culturing T cells with a factor selected from the group consisting of flt3-L, IL-1, IL-3 and c-kit ligand.

[0339] The culturing step as described herein (following contact with an agent as described herein or electroporation) can be very short, e.g., less than 24 hours, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, or 23 hours. The culturing step as further described herein (contact with an agent as described herein) can be longer, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or more days.

[0340] Various terms are used to describe cultured cells. Cell culture generally refers to cells taken from a living organism and grown under controlled conditions. Primary cell culture is a culture of cells, tissues or organs taken directly from an organism before the first subculture. Cells are expanded in culture when they are placed in a growth medium under conditions that facilitate cell growth and / or division, resulting in a larger cell population. When cells are expanded in culture, the proliferation rate of the cells is typically measured by the time required for the number of cells to double, otherwise known as the doubling time.

[0341] Each round of subculture is called a passage. When cells are subcultured, they are said to be passaged. A particular cell population or cell line is sometimes referred to or characterized by the number of times they have been subcultured. For example, a cultured cell population that has been subcultured 10 times may be referred to as a P10 culture. A primary culture, i.e., the first culture after isolating cells from tissue, is called P0. After the first subculture, the cells are described as a secondary culture (P1 or passage 1). After the second subculture, the cells become a tertiary culture (P2 or passage 2), and so on. It will be understood by those skilled in the art that there may be many population doublings during the subculture period; therefore, the population doubling number of a culture is greater than the number of passages. The expansion of cells between subcultures (i.e., the number of population doublings) depends on many factors, including but not limited to the seeding density, substrate, medium, and subculture interval.

[0342] In one embodiment, cells may be cultured for a few hours (about 3 hours) to about 14 days or any integer value of time therebetween. Conditions suitable for T cell culture include an appropriate medium (e.g., Minimum Essential Medium or RPMI Media 1640 or X-vivo 15 (Lonza)) that may contain factors necessary for growth and survival, including serum (e.g., fetal bovine serum or human serum), interleukin-2 (IL-2), insulin, IFN-gamma, IL-4, IL-7, GM-CSF, IL-10, IL-12, IL-15, TGF-beta and TNF-α or any other additive for cell growth known to those of skill in the art. Other additives for cell growth include, but are not limited to, detergents, plasmanate, and reducing agents such as N-acetyl-cysteine ​​and 2-mercaptoethanol. Culture media can include RPMI 1640, AIM-V, DMEM, MEM, α-MEM, F-12, X-Vivo 15 and X-Vivo 20, Optimizer, either serum-free or supplemented with appropriate amounts of serum (or plasma) or a defined set of hormones and / or cytokines in sufficient amounts for T cell growth and expansion, with addition of amino acids, sodium pyruvate and vitamins. Antibiotics, such as penicillin and streptomycin, are included only in experimental cultures and not in cultures of cells to be infused into subjects. Target cells are grown under conditions necessary to support growth, such as an appropriate temperature (e.g., 37° C.) and atmosphere (e.g., air+5% CO2). 2 ) is maintained below

[0343] The medium used to culture T cells can include an agent that can costimulate T cells. For example, an agent that can stimulate CD3 is an antibody to CD3, and an agent that can stimulate CD28 is an antibody to CD28. The cells isolated by the method disclosed herein can be expanded approximately 10-fold, 20-fold, 30-fold, 40-fold, 50-fold, 60-fold, 70-fold, 80-fold, 90-fold, 100-fold, 200-fold, 300-fold, 400-fold, 500-fold, 600-fold, 700-fold, 800-fold, 900-fold, 1000-fold, 2000-fold, 3000-fold, 4000-fold, 5000-fold, 6000-fold, 7000-fold, 8000-fold, 9000-fold, 10,000-fold, 100,000-fold, 1,000,000-fold, 10,000,000-fold, or more. In one embodiment, T cells are expanded in the range of about 20-fold to about 50-fold, or more. In one embodiment, human regulatory T cells are expanded via KT64.86 artificial antigen presenting cells (aAPCs) coated with anti-CD3 antibodies. Methods for expanding and activating T cells can be found in U.S. Patent Nos. 7,754,482, 8,722,400, and 9,555,105, the contents of which are incorporated herein in their entireties.

[0344] In one embodiment, the method of expanding T cells can further include isolating the expanded T cells for further application.In another embodiment, the method of expanding can further include subsequent electroporation of the expanded T cells before culturing.Subsequent electroporation can include introducing a nucleic acid encoding an agent into the expanded T cell population, such as transducing the expanded T cells with a nucleic acid, transfecting the expanded T cells, or electroporating the expanded T cells, and the agent further stimulates the T cells.The agent can stimulate the T cells by further expanding, stimulating effector function or another T cell function, etc.

[0345] H. Methods for Producing Engineered Immune Cells The present disclosure provides methods for producing or generating the modified immune cells of the invention or their precursors (e.g., T cells) for tumor immunotherapy, e.g., adoptive immunotherapy.

[0346] In some embodiments, CAR is introduced into cells by expression vector. The expression vector comprising the nucleic acid sequence encoding the CAR of the present invention is provided herein. Suitable expression vectors include lentivirus vectors, gamma retrovirus vectors, foamy virus vectors, adeno-associated virus (AAV) vectors, adenovirus vectors, engineered hybrid viruses, naked DNA including but not limited to transposon-mediated vectors, such as integrases such as Sleeping Beauty, Piggybak and Phi31. Some other suitable expression vectors include herpes simplex virus (HSV) and retrovirus expression vectors.

[0347] In certain embodiments, the nucleic acid encoding the CAR is introduced into the cell via viral transduction. In certain embodiments, the viral transduction comprises contacting the immune cell or progenitor cell with a viral vector comprising the nucleic acid encoding the CAR. In certain embodiments, the viral vector is an adeno-associated virus (AAV) vector. In certain embodiments, the AAV vector comprises the 5'ITR and 3'ITR from AAV6. In certain embodiments, the AAV vector comprises the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). In certain embodiments, the AAV vector comprises a polyadenylation (polyA) sequence. In certain embodiments, the polyA sequence is the bovine growth hormone (BGH) polyA sequence.

[0348] Adenoviral expression vectors are based on adenovirus and have a low capacity for integration into genomic DNA, but high efficiency for transfection into host cells. Adenoviral expression vectors contain sufficient adenoviral sequences to (a) assist in packaging of the expression vector, and (b) ultimately express CAR in host cells. In some embodiments, the adenoviral genome is a 36 kb linear double-stranded DNA into which a foreign DNA sequence (e.g., a nucleic acid encoding a CAR) may be inserted to replace a large piece of adenoviral DNA to generate the expression vector of the present invention (see, e.g., Danthinne and Imperiale, Gene Therapy (2000) 7(20): 1707-1714).

[0349] Another expression vector is based on adeno-associated virus (AAV) and utilizes the adenovirus conjugation system. This AAV expression vector has a high integration frequency into the host genome. This vector can infect non-dividing cells, and is therefore useful for delivering genes into mammalian cells, for example, in tissue culture or in vivo. AAV vector has a wide host range for infection. Details regarding the generation and use of AAV vectors are described in U.S. Patent Nos. 5,139,941 and 4,797,368.

[0350] Retroviral expression vectors can integrate into host genomes, deliver large amounts of foreign genetic material, infect a wide range of species and cell types, and package into special cell lines.Retroviral vectors are constructed by inserting nucleic acid (e.g., the nucleic acid encoding CAR) into a specific location in the viral genome to produce a virus that is replication-defective.Retroviral vectors can infect a wide variety of cell types, but the integration and stable expression of CAR requires the division of host cells.

[0351] Lentiviral vectors are composite retroviruses derived from lentiviruses and contain other genes with regulatory or structural functions in addition to the common retroviral genes gag, pol and env (see, for example, U.S. Patent Nos. 6,013,516 and 5,994,136). Some examples of lentiviruses include human immunodeficiency virus (HIV-1, HIV-2) and simian immunodeficiency virus (SIV). Lentiviral vectors have been generated by multiple attenuation of HIV pathogenicity genes, for example, genes env, vif, vpr, vpu and nef are deleted, making the vector biologically safe. Lentiviral vectors can infect non-dividing cells and can be used for both in vivo and ex vivo gene transfer and expression, for example, of nucleic acids encoding CAR (see, for example, U.S. Patent No. 5,994,136).

[0352] The expression vector containing the nucleic acid of the present disclosure can be introduced into the host cell by any means known to those skilled in the art. The expression vector may contain a viral sequence for transfection if desired. Alternatively, the expression vector may be introduced by fusion, electroporation, biolistics, transfection, lipofection, etc. The host cell may be grown and expanded in culture before the introduction of the expression vector, followed by treatment suitable for the introduction and integration of the vector. The host cell may then be expanded and screened by a marker present in the vector. Various markers that may be used are known in the art and may include hprt, neomycin resistance, thymidine kinase, hygromycin resistance, etc. As used herein, the terms "cell", "cell line" and "cell culture" may be used interchangeably. In some embodiments, the host cell is an immune cell or a precursor thereof, such as a T cell, a NK cell, or a NKT cell.

[0353] The present invention also provides genetically engineered cells that contain and stably express the CAR of the present disclosure.In some embodiments, the genetically engineered cells are genetically engineered T lymphocytes (T cells), naive T cells (TN), memory T cells (e.g., central memory T cells (TCM), effector memory cells (TEM)), natural killer cells (NK cells), and macrophages that can generate therapeutically relevant progeny.In certain embodiments, the genetically engineered cells are autologous cells.In certain embodiments, the modified cells are resistant to T cell exhaustion.

[0354] Modified cells (e.g., containing CAR) can be produced by stably transfecting an expression vector containing the nucleic acid of the present disclosure into a host cell. Additional methods for generating modified cells of the present disclosure include, but are not limited to, chemical transformation methods (e.g., using calcium phosphate, dendrimers, liposomes, and / or cationic polymers), non-chemical transformation methods (e.g., electroporation, phototransformation, gene electrotransfer and / or hydrodynamic delivery), and / or particle-based methods (e.g., impalefection and / or magnetofection using a gene gun). Transfected cells expressing the CAR of the present disclosure can be expanded ex vivo.

[0355] Physical methods for introducing expression vectors into host cells include calcium phosphate precipitation, lipofection, particle bombardment, microinjection, electroporation, etc. Methods for producing cells containing vectors and / or exogenous nucleic acids are well known in the art. See, for example, Sambrook et al. (2001), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. Chemical methods for introducing expression vectors into host cells include colloidal dispersion systems such as macromolecular complexes, nanocapsules, microspheres, beads, and lipid-based systems including oil-in-water emulsions, micelles, mixed micelles, and liposomes.

[0356] Lipids suitable for use can be obtained from commercial sources. For example, dimyristyl phosphatidylcholine ("DMPC") can be obtained from Sigma, St. Louis, MO; dicetyl phosphate ("DCP") can be obtained from K & K Laboratories (Plainview, NY); cholesterol ("Choi") can be obtained from Calbiochem-Behring; dimyristyl phosphatidylglycerol ("DMPG") and other lipids may be obtained from Avanti Polar Lipids, Inc. (Birmingham, AL). Stock solutions of lipids in chloroform or chloroform / methanol can be stored at about -20°C. Chloroform can be used as the sole solvent since it evaporates more readily than methanol. "Liposome" is a generic term that encompasses a variety of single and multilamellar lipid vesicles formed by the formation of enclosed lipid bilayers or aggregates. Liposomes can be characterized as having a vesicular structure with a phospholipid bilayer membrane and an internal aqueous medium. Multilamellar liposomes have multiple lipid layers separated by aqueous medium. They form spontaneously when phospholipids are suspended in an excess of aqueous solution. Before the formation of closed structures, the lipid components undergo self-rearrangement, trapping water and dissolved solutes between the lipid bilayers (Ghosh et al., 1991 Glycobiology 5: 505-10). Also included are compositions that have structures in solution that differ from the normal vesicular structure. For example, lipids may adopt micellar structures or simply exist as non-uniform aggregates of lipid molecules. Lipofectamine-nucleic acid complexes are also envisioned.

[0357] Regardless of the method used to introduce exogenous nucleic acid into host cells or the method used to expose cells to the inhibitor of the present invention, various assays can be carried out to confirm the presence of nucleic acid in host cells.Such assays include, for example, molecular biological assays well known to those skilled in the art, such as Southern blotting, Northern blotting, RT-PCR and PCR; biochemical assays, such as detecting the presence or absence of specific peptides, for example, by immunological means (ELISA and Western blot), or by the assays described herein to identify agents that fall within the scope of the present invention.

[0358] In one embodiment, the nucleic acid introduced into the host cell is RNA. In another embodiment, the RNA is mRNA, including in vitro transcribed RNA or synthetic RNA. RNA can be produced by in vitro transcription using a template generated by polymerase chain reaction (PCR). Interesting DNA from any source can be directly converted into a template for in vitro mRNA synthesis by PCR using suitable primers and RNA polymerase. The source of DNA can be, for example, genomic DNA, plasmid DNA, phage DNA, cDNA, synthetic DNA sequence or any other suitable DNA source.

[0359] PCR may be used to generate templates for in vitro transcription of mRNA that is then introduced into cells. Methods for performing PCR are well known in the art. Primers for use in PCR are designed to have a region that is substantially complementary to the DNA region to be used as a template for PCR. "Substantially complementary" as used herein refers to a nucleotide sequence that is complementary to most or all of the bases in the primer sequence. A substantially complementary sequence is capable of annealing or hybridizing with the DNA target of interest under the annealing conditions used for PCR. Primers can be designed to be substantially complementary to any portion of the DNA template. For example, primers can be designed to amplify gene portions that are normally transcribed in cells (open reading frames), including 5' UTRs and 3' UTRs. Primers can also be designed to amplify portions of genes that code for a particular domain of interest. In one embodiment, primers are designed to amplify the coding region of human cDNA, including all or part of 5' UTRs and 3' UTRs. Primers useful for PCR are generated by synthetic methods well known in the art. A "forward primer" is a primer that contains a nucleotide region that is substantially complementary to nucleotides on a DNA template that is upstream of the DNA sequence to be amplified. "Upstream" is used herein to refer to the 5th position of the DNA sequence to be amplified relative to the coding strand. A "reverse primer" is a primer that contains a nucleotide region that is substantially complementary to a double-stranded DNA template that is downstream of the DNA sequence to be amplified. "Downstream" is used herein to refer to the 3' position of the DNA sequence to be amplified relative to the coding strand.

[0360] Chemical structures capable of promoting RNA stability and / or translation efficiency may also be used. The RNA preferably has a 5' UTR and a 3' UTR. In one embodiment, the 5' UTR is zero to 3000 nucleotides long. The length of the 5' UTR and 3' UTR sequences to be added to the coding region can be altered by different methods, including but not limited to designing primers for PCR that anneal to different regions of the UTR. Using this approach, one skilled in the art can modify the length of the 5' UTR and 3' UTR required to achieve optimal translation efficiency after transfection of the transcribed RNA.

[0361] 5'UTR and 3'UTR can be the naturally occurring endogenous 5'UTR and 3'UTR for the gene of interest. Alternatively, the UTR sequence that is not endogenous to the gene of interest can be added by incorporating the UTR sequence in the forward primer and reverse primer, or by any other modification of the template. The use of the UTR sequence that is not endogenous to the gene of interest can be useful for modifying the stability and / or translation efficiency of RNA. For example, it is known that AU-rich elements in 3'UTR sequence can reduce the stability of mRNA. Therefore, 3'UTR can be selected or designed to increase the stability of transcribed RNA based on the properties of UTR that are well known in the art.

[0362] In one embodiment, the 5' UTR can contain the Kozak sequence of the endogenous gene. Alternatively, if a 5' UTR that is not endogenous to the gene of interest is added by PCR as described above, the consensus Kozak sequence can be redesigned by adding a 5' UTR sequence. Although the Kozak sequence can increase the translation efficiency of some RNA transcripts, it does not appear to be necessary for all RNAs to allow efficient translation. The necessity of the Kozak sequence for many mRNAs is known in the art. In another embodiment, the 5' UTR can be derived from an RNA virus whose RNA genome is stable in cells. In another embodiment, various nucleotide analogs can be used in the 3' UTR or 5' UTR to prevent exonuclease degradation of mRNA.

[0363] To allow RNA synthesis from a DNA template without the need for gene cloning, a transcription promoter should be attached to the DNA template upstream of the sequence to be transcribed.When a sequence that functions as a promoter for RNA polymerase is added to the 5' end of the forward primer, the RNA polymerase promoter will be incorporated upstream of the open reading frame to be transcribed in the PCR product.In one embodiment, the promoter is a T7 polymerase promoter as described elsewhere herein.Other useful promoters include, but are not limited to, T3 and SP6 RNA polymerase promoters.The consensus nucleotide sequences for T7, T3 and SP6 promoters are known in the art.

[0364] In one embodiment, mRNA has both a cap on the 5' end and a 3' poly(A) tail, which determine ribosome binding, translation initiation and the stability of mRNA in cells.On circular DNA templates, such as plasmid DNA, RNA polymerase produces long concatemeric products that are not suitable for expression in eukaryotic cells.Transcription of plasmid DNA linearized at the end of 3'UTR produces mRNA of normal size, which is not effective for eukaryotic transfection even when it is polyadenylated after transcription.

[0365] On a linear DNA template, phage T7 RNA polymerase can extend the 3' end of a transcript beyond the final base of the template (Schenborn and Mierendorf, Nuc Acids Res., 13:6223-36 (1985); Nacheva and Berzal-Herranz, Eur. J. Biochem., 270:1485-65 (2003)).

[0366] The polyA / T segment of the transcribed DNA template can be generated during PCR by using a reverse primer containing a polyT tail (size can be 50-5000T), such as a 100T tail, or after PCR by any other method, including but not limited to DNA ligation or in vitro recombination. The poly(A) tail also provides stability to RNAs and reduces their degradation. In general, the length of the poly(A) tail positively correlates with the stability of the transcribed RNA. In one embodiment, the poly(A) tail is 100-5000 adenosines.

[0367] The poly(A) tail of the RNA can be further extended by the use of a poly(A) polymerase, such as Escherichia coli (E. coli) polyA polymerase (E-PAP), after in vitro transcription. In one embodiment, increasing the length of the poly(A) tail from 100 nucleotides to 300-400 nucleotides results in an approximately two-fold increase in the translation efficiency of the RNA. In addition, attachment of different chemical groups to the 3' end can increase the stability of the mRNA. Such attachments can contain modified / artificial nucleotides, aptamers and other compounds. For example, poly(A) polymerase can be used to incorporate ATP analogs into the poly(A) tail. The ATP analogs can further increase the stability of the RNA.

[0368] The 5' cap also provides stability to the RNA molecule. In a preferred embodiment, the RNA produced by the methods disclosed herein comprises a 5' cap. The 5' cap is provided using techniques known in the art and described herein (Cougot, et al., Trends in Biochem. Sci., 29:436-444 (2001); Stepinski, et al., RNA, 7:1468-95 (2001); Elango, et al., Biochim. Biophys. Res. Commun., 330:958-966 (2005)).

[0369] In some embodiments, the RNA is electroporated into cells as in vitro transcribed RNA. Any solute suitable for cell electroporation can be included, which can contain factors that promote cell permeability and viability, such as sugars, peptides, lipids, proteins, antioxidants, and detergents.

[0370] In some embodiments, the nucleic acid encoding the CAR of the present disclosure will be RNA, for example, in vitro synthesized RNA. Methods for in vitro synthesis of RNA are known in the art; any known method can be used to synthesize RNA comprising a sequence encoding a CAR. Methods for introducing RNA into a host cell are known in the art. See, for example, Zhao et al. Cancer Res. (2010) 15: 9053. Introducing RNA comprising a nucleotide sequence encoding a CAR into a host cell can be performed in vitro, ex vivo, or in vivo. For example, host cells (e.g., NK cells, cytotoxic T lymphocytes, etc.) can be electroporated in vitro or ex vivo with RNA comprising a nucleotide sequence encoding a CAR.

[0371] The disclosed methods can be applied to modulating T cell activity in basic research and therapy in the areas of cancer, stem cells, acute and chronic infections, and autoimmune diseases, including assessing the ability of genetically modified T cells to kill targeted cancer cells.

[0372] The method also provides the ability to control the expression level over a wide range, for example by varying the amount of promoter or input RNA, allowing expression levels to be individually adjusted.Furthermore, the PCR-based technique of mRNA production makes it extremely easy to design mRNAs with different structures and combinations of their domains.

[0373] One advantage of the RNA transfection method of the present invention is that RNA transfection is essentially transient and vector-free.RNA transgenes can be delivered to lymphocytes and expressed therein after a short period of in vitro cell activation as a minimal expression cassette without the need for any additional viral sequences.Under these conditions, the integration of transgenes into the host cell genome is unlikely to occur.Due to the transfection efficiency of RNA and the ability of RNA to uniformly modify the entire lymphocyte population, cloning of cells is unnecessary.

[0374] Genetic modification of T cells with in vitro transcribed RNA (IVT-RNA) utilizes two different strategies, both of which have been successfully tested in various animal models. Cells are transfected with in vitro transcribed RNA by lipofection or electroporation. To achieve long-term expression of the transferred IVT-RNA, it is desirable to stabilize the IVT-RNA using various modifications.

[0375] Several IVT vectors are known from the literature that are utilized in standard procedures as templates for in vitro transcription and that have been genetically modified to produce stabilized RNA transcripts. Currently, the protocols used in the art are based on plasmid vectors with the following structure: a 5' RNA polymerase promoter that allows RNA transcription, followed by the gene of interest flanked by untranslated regions (UTRs) either at the 3' and / or 5', as well as a 3' polyadenylation cassette containing 50-70 A nucleotides. Prior to in vitro transcription, the circular plasmid is linearized downstream of the polyadenylation cassette by a type II restriction enzyme (the recognition sequence corresponds to the cleavage site). The polyadenylation cassette thus corresponds to the subsequent poly(A) sequence in the transcript. As a result of this procedure, some nucleotides remain as part of the enzyme cleavage site after linearization, extending or masking the poly(A) sequence at the 3' end. It is unclear whether this non-physiological overhang affects the amount of protein produced in cells from such a construct.

[0376] In another aspect, RNA construct is delivered into cell by electroporation.See, for example, the formulation and methodology of electroporation of nucleic acid construct into mammalian cell as taught in US 2004 / 0014645, US 2005 / 0052630A1, US 2005 / 0070841A1, US 2004 / 0059285A1, US 2004 / 0092907A1.Various parameters, including the electric field strength required for electroporation of any known cell type, are generally known from related research literature and numerous patents and applications in this field.See, for example, US Patent No. 6,678,556, US Patent No. 7,171,264, and US Patent No. 7,173,116. Devices for therapeutic applications of electroporation are commercially available, for example, MedPulser™ DNA Electroporation Therapy System (Inovio / Genetronics, San Diego, Calif.), U.S. Pat. No. 6,567,694; U.S. Pat. No. 6,516,223, U.S. Pat. No. 5,993,434, U.S. Pat. No. 6,181,964, U.S. Pat. No. 6,241,701, and U.S. Pat. No. 6,233,482; electroporation can also be used for in vitro transfection of cells, for example, as described in US20070128708A1. Electroporation can also be utilized to deliver nucleic acid into cells in vitro. Thus, electroporation-mediated administration of nucleic acid, including expression constructs, into cells using any of the many available devices and electroporation systems known to those skilled in the art represents an exciting new means to deliver RNA of interest to target cells.

[0377] I. Pharmaceutical Compositions and Formulations In another aspect, the present disclosure provides pharmaceutical compositions comprising any therapeutically effective amount of modified cells envisaged herein.Also provided are immune cell populations of the present invention, compositions containing such cells and / or enriched with such cells, for example, compositions in which CAR expressing cells account for at least 50%, 60%, 70%, 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more of the total cells or a certain type of cells, such as T cells or CD8+ cells or CD4+ cells, in the composition.Compositions are, among others, pharmaceutical compositions and formulations for administration, such as for adoptive cell therapy.Also provided are therapeutic methods for administering cells and compositions to subjects, for example, patients.

[0378] Also provided are compositions that contain cells for administration, including pharmaceutical compositions and formulations, such as unit dose form compositions that contain the number of cells for administration at a given dose or fraction thereof.Pharmaceutical compositions and formulations generally contain one or more optional pharmaceutically acceptable carriers or excipients.In some embodiments, the composition contains at least one additional therapeutic agent.

[0379] The term "pharmaceutical formulation" refers to a preparation in a form that allows the biological activity of the active ingredient contained therein to be effective, and that does not contain additional ingredients that are unacceptably toxic to the subject to which the formulation will be administered. "Pharmaceutically acceptable carrier" refers to an ingredient in a pharmaceutical formulation, other than the active ingredient, that is non-toxic to the subject. Pharmaceutically acceptable carriers include, but are not limited to, buffers, excipients, stabilizers, or preservatives. In some aspects, the choice of carrier is determined, in part, by the particular cell and / or by the method of administration. Thus, there is a wide variety of suitable formulations. For example, the pharmaceutical composition can contain a preservative. Suitable preservatives can include, for example, methylparaben, propylparaben, sodium benzoate, and benzalkonium chloride. In some aspects, a mixture of two or more preservatives is used. The preservative or mixtures thereof are typically present in an amount of about 0.0001% to about 2% by weight of the total composition. Carriers are described, for example, in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980).Pharmaceutically acceptable carriers are generally nontoxic to recipients at the dosages and concentrations employed and include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzylammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl alcohol or benzyl alcohol; alkyl parabens such as methylparaben or propylparaben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose, or sorbitol; salt-forming counterions such as sodium; metal complexes (e.g., Zn-protein complexes); and / or non-ionic surfactants such as polyethylene glycol (PEG).

[0380] In some aspects, a buffering agent is included in the composition. Suitable buffering agents include, for example, citric acid, sodium citrate, phosphoric acid, potassium phosphate, and various other acids and salts. In some aspects, a mixture of two or more buffering agents is used. The buffering agent or mixture is typically present in an amount of about 0.001% to about 4% by weight of the total composition. Methods for preparing administrable pharmaceutical compositions are known. Exemplary methods are described in more detail, for example, in Remington: The Science and Practice of Pharmacy, Lippincott Williams & Wilkins; 21st ed. (May 1, 2005).

[0381] The formulation may comprise an aqueous solution. The formulation or composition may also contain more than one active ingredient useful for the particular indication, disease, or condition treated by the cells, preferably with complementary activities to the cells, each of which does not adversely affect the other. Such active ingredients are suitably present in combination in an amount effective for the intended purpose. Thus, in some embodiments, the pharmaceutical composition further comprises other pharma- ceutical active agents or drugs, such as chemotherapeutic agents, such as asparaginase, busulfan, carboplatin, cisplatin, daunorubicin, doxorubicin, fluorouracil, gemcitabine, hydroxyurea, methotrexate, paclitaxel, rituximab, vinblastine, and / or vincristine. The pharmaceutical composition, in some embodiments, contains the cells in an amount effective to treat or prevent the disease or condition, such as a therapeutically or prophylactically effective amount. The therapeutic or prophylactic effectiveness is, in some embodiments, monitored by periodically evaluating the subject being treated. The desired dosage can be delivered by administration of a single bolus of cells, by administration of multiple boluses of cells, or by administration of continuous infusions of cells.

[0382] Formulations include those for oral, intravenous, intraperitoneal, subcutaneous, intrapulmonary, transdermal, intramuscular, intranasal, buccal, sublingual, or suppository administration. In some embodiments, the cell population is administered parenterally. The term "parenteral" as used herein includes intravenous, intramuscular, subcutaneous, rectal, vaginal, and intraperitoneal administration. In some embodiments, the cells are administered to the subject using peripheral systemic delivery by intravenous, intraperitoneal, or subcutaneous injection. The composition is provided in some embodiments as a sterile liquid preparation, such as an isotonic aqueous solution, suspension, emulsion, dispersion, or viscous composition, which in some aspects may be buffered to a selected pH. Liquid preparations are usually easier to prepare than gels, other viscous compositions, and solid compositions. Additionally, liquid compositions are somewhat more convenient to administer, especially for administration by injection. On the other hand, viscous compositions can be formulated within a suitable viscosity range to provide a longer contact period with a particular tissue. The liquid or viscous compositions can include a carrier, which can be a solvent or dispersion medium containing, for example, water, saline, phosphate buffered saline, polyol (e.g., glycerol, propylene glycol, liquid polyethylene glycol), and suitable mixtures thereof.

[0383] Sterile injectable solutions can be prepared by incorporating the cells in a solvent, for example, in a solvent mixed with a suitable carrier, diluent or excipient, such as sterile water, physiological saline, glucose, dextrose, etc. The composition can contain auxiliary substances, such as wetting agents, dispersing or emulsifying agents (e.g., methylcellulose), pH buffering agents, gelling or viscosity enhancing additives, preservatives, flavoring agents and / or coloring agents, depending on the desired route of administration and preparation. In some aspects, standard textbooks may be consulted for preparing appropriate preparations.

[0384] Various additives can be added to enhance the stability and sterility of the composition, including antibacterial preservatives, antioxidants, chelating agents, and buffers.Prevention of microbial action can be ensured by various antibacterial and antifungal agents, such as paraben, chlorobutanol, phenol, and sorbic acid.Prolonged absorption of injectable pharmaceutical dosage forms can be achieved by using agents that delay absorption, such as aluminum monostearate and gelatin.

[0385] Formulations to be used for in vivo administration are generally sterile. Sterility may be readily accomplished, for example, by filtration through sterile filtration membranes.

[0386] The contents of the articles, patents and patent applications, and all other documents and electronically available information mentioned or cited in this specification are incorporated herein by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference. Applicants reserve the right to physically incorporate into this application any and all materials and information from any such articles, patents, patent applications, or other physical and electronic documents.

[0387] Although the present invention has been described with reference to specific embodiments thereof, it should be understood by those skilled in the art that various modifications and equivalent substitutions may be made without departing from the true spirit and scope of the present invention. It will be readily apparent to those skilled in the art that other suitable modifications and adaptations of the methods described herein may be made using appropriate equivalents without departing from the scope of the embodiments disclosed herein. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of the appended claims. Although specific embodiments have been described in detail herein, the same will be more clearly understood by reference to the following examples, which are included for illustrative purposes only and are not intended to be limiting. EXAMPLES

[0388] Experimental Examples The invention will now be described with reference to the following examples, which are provided for purposes of illustration only, and the invention is not limited to these examples, but includes all variations that are evident as a result of the teachings provided herein.

[0389] material and method Cell lines and primary human T lymphocyte cultures: Primary human CD4 and CD8 T cells were isolated from healthy volunteer donors by leukapheresis followed by negative selection using the RosetteSep kit (Stem Cell Technologies). All specimens were collected under a University Review Board-approved protocol, and written informed consent was obtained from each donor. Primary human CD4 and CD8 T cell mixtures (1:1) were stimulated with anti-CD3 / CD28 Dynabeads (Life Technologies).

[0390] CAR constructs and lentiviral transduction: A PSCA CAR composed of scFv from a humanized anti-PSCA Ab (2B3) was constructed and cloned into the retroviral vector MSGV. The scFv domain against PSCA was synthesized and / or amplified by PCR, linked to the CD8 transmembrane domain and the 4-1BB, CD28, ICOS, or ICOS.YMNM, and CD3 zeta intracellular signaling domains, and subcloned into a pTRPE lentiviral vector. T cells were transduced with the lentiviral vector at an MOI of 5.

[0391] Flow cytometry: Transduction efficiency of transduced cells was determined using flow cytometry after staining the transduced cells with biotin-labeled polyclonal anti-mouse F(ab)2 antibody (Jackson Immunoresearch). The following antibody was used for flow cytometry experiments: PE-conjugated streptavidin. Data were acquired on a FACSCalibur (BC Biosciences) and analyzed by FlowJo.

[0392] CD107a assay: E:T was 1:2 (1 × 10 5 Effectors: 2×10 5 Cells were plated in 160 μL of R10 medium in 96-well plates at 100 μl / well. Of note, 20 μL of phycoerythrin-labeled anti-CD107a Ab was added and the plate was incubated at 37° C. for 1 h, after which Golgi Stop (2 mL of Golgi Stop in 3 mL of R10 medium, 20 mL / well; BD Biosciences, 51-2092 KZ) was added and the plate was incubated for an additional 2.5 h. Then, 5 mL of FITC-anti-CD8 and 5 mL of streptavidin-allophycocyanin (APC)-anti-CD3 were added and incubated at 37° C. for 30 min. After incubation, samples were washed with FACS buffer and analyzed by flow cytometry.

[0393] Enzyme-linked immunosorbent assay (ELISA): Wash target cells and culture at 1 × 10 cells in R10 medium. 6 Of note, 100 μL of each target cell type was added in triplicate to a 96-well round-bottom plate (Corning). Effector T cells were washed and suspended at 1 × 10 6 The T cells were resuspended at 100 cells / mL, and then 100 μL of the T cells were combined with the target cells in the indicated wells. The plates were incubated at 37° C. for 18 to 24 hours. After incubation, the supernatants were collected and subjected to ELISA assays (eBioscience).

[0394] Luciferase-based cytolytic T cell (CTL) assay: Briefly, click beetle green luciferase (CBG)-T2A-eGFP was transduced into PSCA tumor cells by lentivirus and selected for expression of GFP. Tumor cells were incubated with different ratios of T cells at 37°C for 8 hours. Of note, 100 mL of the mixture was transferred to a 96-well white luminometer plate, 100 mL of substrate was added, and luminescence was immediately determined. Results are reported as percent killing based on luciferase activity in wells with tumor but no T cells. (% killing=100-((RLU from wells with effector and target cell co-cultures) / (RLU from wells with target cells)×100).

[0395] Example 1: Various prostate stem cell antigen (PSCA) specific CARs were generated herein. The antigen binding domain was derived from a humanized anti-PSCA antibody (2B3) (US Patent Application Publication No. US2010 / 0297004, the contents of which are incorporated herein by reference in their entirety) (Figure 1). 2B3 scFv was used in combination with various intracellular domains, including 4-1BB and CD3 zeta (2B3.BBZ CAR), CD28 and CD3 zeta (2B3.28Z CAR), ICOS and CD3 zeta (2B3.ICOSZ CAR), and mutant ICOS (ICOS.YMNM CAR) and CD3 zeta (2B3.ICOS.YMNM CAR). PSCA CARs were also generated, including PD1-CD28 switch receptor, TGFbR / IL12R switch receptor and dominant negative receptor (TGFbRDN). Dual CARs were also developed, including specificity for PSCA and PSMA. PSCA CARs have also been used in combination with bispecific antibodies (e.g., aPD-L1 / CD28 or aTGFbRII-CD28).

[0396] CAR expression was measured in T cells co-electroporated with in vitro transcribed RNA of PSCA CAR (2B3.BBZ) and PMSA CAR (J591.BBZ) (Figure 2, top panel). PD-L1-Fc staining of PSCA CAR co-electroporated with bispecific antibody 10A5-1412 (aPDL1-aCD28 bispecific Ab) or TGFB3-1412 (aTGFbRII-aCD28 bispecific Ab) is shown in the bottom panel of Figure 2.

[0397] CD107a was measured in RNA co-electroporated T cells stimulated with PC3-PSCA-PSMA or K562 (Figure 3).

[0398] T cells lentivirally transduced with PSCA(2B3) CAR with a mutant ICOS signaling domain (ICOS.YMNM) showed improved lytic potential, reduced in vitro cytokine production, and in vivo antitumor activity equivalent to 4-1BB signaling PSCA CAR (Figure 4 and Figure 6). PSCA CARs with either ICOS or ICOS.YMNM signaling domains were constructed and cloned into lentiviral vectors. CAR expression levels were equivalent to either 4-1BB or CD28 signaling domain CARs (Figure 4, top). Cytokine production (IFN-gamma) was measured following stimulation with the PSCA-positive cell lines PC3.PSCA.PMSA.CBG or PC3.PSCA.PMSA.CBG.PD-L1 (Figure 4, bottom panel).

[0399] The results of a CD107a assay for T cells expressing PSCA CARs with either ICOS or ICOS.YMNM signaling domains are shown in Figure 5A. Figure 5B illustrates the results of a killing assay of T cells expressing PSCA CARs with either ICOS or ICOS.YMNM signaling domains against the tumor line PC3-PSCA.

[0400] FIG. 6 illustrates the BLI results of the PC3-PSCA-CBG-PDL1 tumor model. For lentiviral-transduced (LVV TD) T cells, 1e per mouse was administered at 21 days post-tumor inoculation. 6 The cells were transduced intravenously (iv). After subcutaneous injection of the various PSCA CARs, tumor BLI (mean radiance) (Figure 7) and tumor size (Figure 8) were measured.

[0401] Bispecific CARs were also generated herein. The vectors used in the study encoding PSMA (2F5 scFv)-targeted CAR and PSCA-targeted CAR linked by a Gly4Ser element are depicted in Figure 9A. Surface expression of CAR on lentiviral-transduced CAR T cells at the end of the first expansion is depicted in Figure 9B. The percentage of lentiviral-transduced CAR T cells expressing PSMA or PSCA-CAR, or bispecific CAR, was measured by staining with human recombinant PSMA-Fc and PSCA-His proteins and analyzed by flow cytometry (Figure 9C). CAR T cells expressing the indicated scFv were co-cultured with targets for 4 hours, and the percentage of CD107a expression was quantified on CD8-positive cells (Figure 9D).

[0402] Bispecific CAR T cells were co-cultured with PC3-PSCA cells (effector:target=1:1). After 24 hours of co-culture, supernatants were obtained and cytokine production was analyzed by ELISA (Figure 10A). Bispecific CAR T cells were tested for cytolytic activity against PC3-PSCA cells at different E:T ratios for 8 hours (Figure 10B). Bispecific CAR T cells were co-cultured with PC3-PSMA cells (effector:target=1:1). After 24 hours of co-culture, supernatants were obtained and cytokine production was analyzed by ELISA (Figure 10C). Bispecific CAR T cells were tested for cytolytic activity against PC3-PSMA cells at different E:T ratios for 8 hours (Figure 10D).

[0403] Figures 11A-11B illustrate the finding that TGFbR-IL12R switch receptor can enhance T cell function. Figure 11A (top right panel) shows IFN-gamma production of NK cells transfected with TGFbR-IL12R co-cultured with K562 with or without TGFb1 in culture. Figure 11A (bottom right panel) shows pSmad staining of TGFbR-IL12R switch receptor transfected T cells after stimulation with TGFbeta. Figure 11B shows cytokine production of NY-ESO-1 positive tumors stimulated with NY-ESO-1 TCR transduced T cells co-transfected with TGFbR-IL12R switch receptor.

[0404] Other Aspects The recitation of a list of elements in any definition of a variable herein includes that definition of the variable as any single element or combination (or subcombination) of the listed elements. The recitation of an embodiment herein includes that embodiment as any single embodiment or in combination with any other embodiment or portion thereof.

[0405] The disclosures of any and all patents, patent applications, and publications cited herein are incorporated herein by reference in their entirety. Although the present invention has been disclosed with reference to specific embodiments, it is apparent that other embodiments and variations of the present invention may be devised by those skilled in the art without departing from the spirit and scope of the present invention. The appended claims are intended to be construed to cover all such embodiments and equivalent variations.

Claims

1. A chimeric antigen receptor (CAR) comprising an antigen-binding domain capable of binding to prostate stem cell antigen (PSCA), a transmembrane domain, and an intracellular domain, and comprising the amino acid sequence of SEQ ID NO: 21, 23, 25, or 27.

2. A nucleic acid comprising a polynucleotide sequence encoding the CAR of claim 1.

3. A vector comprising the nucleic acid of claim 2.

4. (a) is an expression vector; and / or (b) selected from the group consisting of a DNA vector, an RNA vector, a plasmid, a lentiviral vector, an adenoviral vector, an adeno-associated viral vector, and a retroviral vector; The vector of claim 3.

5. A modified immune cell or a precursor thereof comprising the CAR of claim 1, the nucleic acid of claim 2, or the vector of claim 3.

6. The modified cell of claim 5, further comprising a PSMA-CAR, wherein the PSMA-CAR comprises an antigen-binding domain, a transmembrane domain, and an intracellular domain.

7. 7. The modified cell of claim 5 or 6, further comprising a switch receptor.

8. A modified cell described in any one of claims 5 to 7, which is a modified T cell.

9. A pharmaceutical composition comprising a therapeutically effective amount of the modified cells of any one of claims 5 to 8.

10. A pharmaceutical composition for treating a disease in a subject in need thereof, comprising an effective amount of the modified cells of any one of claims 5 to 8.

11. (a) the disease is cancer; (b) the disease is cancer and the cancer is prostate cancer; or (c) the disease is cancer, and the cancer is metastatic castration-resistant prostate cancer; 11. The pharmaceutical composition of claim 10.

12. (a) the modified T cell further comprises a dominant negative receptor; and / or (b) the engineered T cell further comprises a switch receptor; A pharmaceutical composition according to any one of claims 9 to 11.

13. The pharmaceutical composition according to any one of claims 9 to 12, characterized in that it is used in combination with lymphodepleting chemotherapy.

14. The pharmaceutical composition of any one of claims 9 to 13, wherein the subject is a human.

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