Virus inactivation method, virus inactivation device, and air blower equipped with the virus inactivation device

Abstract

To inactivate a virus existing in the air or a virus deposited on an article.SOLUTION: Plant-derived waste containing polyphenols or its extract is irradiated with light to produce hydroxide radicals. The produced hydroxide radicals are used to inactivate a virus included in an object of interest.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a virus inactivation method, a virus inactivation device, and a blower equipped with the virus inactivation device, for example, to viruses present in the air or viruses attached to clothing. [Background technology]

[0002] Diseases caused by viruses have a significant impact on the world, not only as a matter of health but also as an economic problem. For example, COVID-19, influenza virus, norovirus, and adenovirus infect humans and cause a variety of symptoms such as fever, chills, diarrhea, headache, sore throat, joint and muscle pain, cough, and runny nose. Furthermore, viruses that infect livestock, such as avian influenza and swine influenza, have also caused cases of human infection, leading to economic damage because infected livestock must be culled.

[0003] Patent Document 1 discloses a technique for generating charged water particles containing radicals by electrostatic atomizing water, in which the charged water particles react with at least one of ammonia, acetaldehyde, and acetic acid to decompose at least one of these substances and remove airborne bacteria. The technique in Patent Document 1 is based on the method for decomposing odor components using charged water particles and the apparatus for decomposing odor components disclosed in Patent Document 2.

[0004] Incidentally, Non-Patent Document 1 discloses a technology that generates reactive oxygen species by irradiating waste extracts from wine production with light, and then uses these generated reactive oxygen species to kill bacteria and fungi. In Non-Patent Document 1, white grape pomace and white wine lees were used as waste from wine production, and the generation of reactive oxygen species and antibacterial activity were confirmed in both cases. [Prior art documents] [Patent Documents]

[0005] [Patent Document 1] Japanese Patent Publication No. 2015-119883 [Patent Document 2] Patent No. 5764752 [Non-patent literature]

[0006] [Non-Patent Document 1] Biocontrol Sci, 21(2):113-121, 2016 [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] However, no technology is known for inactivating viruses using plant-derived waste, such as waste from food processing like wine production, and there has been a great need for a system that can effectively utilize plant-derived waste. Therefore, in view of the above circumstances, the present invention aims to provide a virus inactivation method, a virus inactivation device, and a blower equipped with the virus inactivation device that effectively utilize plant-derived waste, such as waste from wine production, to treat viruses present in the air and viruses attached to objects. [Means for solving the problem]

[0008] As a result of diligent research conducted by the inventors to achieve the above-mentioned objectives, they discovered that reactive oxygen species exhibiting an inactivating effect against viruses can be generated by irradiating plant-derived waste containing polyphenols or extracts thereof with light, thus completing the present invention.

[0009] This invention encompasses the following: (1) A method for inactivating viruses, comprising the steps of: irradiating plant-derived waste containing polyphenols or an extract thereof with light to generate hydroxyl radicals; and inactivating viruses contained in the waste to be treated with the generated hydroxyl radicals. (2) The virus inactivation method according to (1), characterized in that the plant-derived waste is waste from wine production. (3) The virus inactivation method according to (2), characterized in that the waste in wine production is the pomace and / or lees after grape pressing. (4) Irradiate the above-mentioned plant-derived waste or its extract with light at 10 to 1000 mW / cm². 2 The method for inactivating a virus according to (1), characterized in that the interval is 2 to 30 seconds. (5) The method for inactivating a virus according to (1), characterized by spraying a solution containing plant-derived waste or an extract thereof and irradiating the mist-like solution with light. (6) A radical generation unit comprising plant-derived waste containing polyphenols or an extract thereof, which generates hydroxyl radicals from the plant-derived waste or an extract thereof, The radical generation unit comprises a light irradiation unit that irradiates the above-mentioned plant-derived waste or its extract with light, A virus inactivation device that generates hydroxyl radicals by irradiating the above-mentioned plant-derived waste or its extract from a radical generation unit with light from a light irradiation unit, and inactivates viruses contained in the treated material with the generated hydroxyl radicals. (7) The virus inactivation apparatus according to (6), characterized in that the plant-derived waste is waste from wine production. (8) The virus inactivation apparatus according to (7), characterized in that the waste in wine production is pomace and / or lees after grape pressing. (9) The above-mentioned light irradiation unit irradiates plant-derived waste or its extract with light at a rate of 10 to 1000 mW / cm². 2 The virus inactivation device according to (6), characterized in that the time is set to 2 to 30 seconds. (10) The radical generation unit is equipped with a spraying device that sprays a solution containing plant-derived waste or an extract thereof, The virus inactivation device according to (6), characterized in that the light irradiation unit irradiates light onto the mist-like solution sprayed from the spraying device. (11) A ventilation device comprising an air passage having an air intake port and an exhaust port, and a virus inactivation device as described in any of (6) to (10) above, characterized in that air that has come into contact with hydroxyl radicals generated by the virus inactivation device is exhausted from the exhaust port in the air passage. (12) The radical generation unit in the virus inactivation device is detachably arranged, and the ventilation device according to (11).

Advantages of the Invention

[0010] According to the virus inactivation method of the present invention, viruses present in the air or viruses attached to objects can be effectively inactivated by a simple procedure. Further, the virus inactivation device according to the present invention can effectively inactivate viruses in the air or viruses attached to objects with a simple configuration. Furthermore, the ventilation device according to the present invention can supply air in a state where the existing viruses are inactivated from the exhaust port by including the virus inactivation device.

Brief Description of the Drawings

[0011] [Figure 1] It is a schematic configuration diagram of a virus inactivation device to which the present invention is applied. [Figure 2] It is a schematic configuration diagram showing another example of a virus inactivation device to which the present invention is applied. [Figure 3] It is a schematic configuration diagram of a ventilation device to which the present invention is applied. [Figure 4] It is a schematic configuration diagram showing another example of a ventilation device to which the present invention is applied. [Figure 5] It is a schematic configuration diagram showing still another example of a ventilation device to which the present invention is applied. [Figure 6] It is a schematic configuration diagram of a decontamination device including a virus inactivation device to which the present invention is applied.

Embodiments for Carrying Out the Invention

[0012] Hereinafter, the present invention will be described in detail with reference to the drawings.

[0013] The virus inactivation method according to the present invention utilizes hydroxyl radicals generated by irradiating plant-derived waste containing polyphenols or extracts thereof with light. Here, plant-derived waste containing polyphenols can be, for example, the residue (press residue) after pressing a plant containing polyphenols, the residue after distillation of a plant containing polyphenols, and the residue after solvent extraction of a plant containing polyphenols.

[0014] Examples of polyphenols include catechins (catechin, gallocatechin, epicatechin, epigallocatechin, catechin gallate, gallocatechin gallate, epicatechin gallate, epigallocatechin gallate, etc.); catechin polymers; flavonols (quercetin, myricetin, kaempferol, etc.) and their glycosides; flavones (avigenin, chrysin, luteolin, etc.); isoflavones (daidzein, genistein, etc.) and their glycosides; flavanones (naringenin, hesperetin, etc.) and their glycosides; anthocyanidins (cyanidin, delphinidin, pelargonidin, etc.) and their glycosides; chalcones; curcumins; lignans; coumarins; phenylcarboxylic acids, etc.

[0015] Plants containing polyphenols are not particularly limited, but examples include plants containing anthocyanins such as grapes, purple sweet potatoes, blueberries, bilberries, mulberries, blackberries, haskap berries, and blackcurrants; plants containing catechins such as green tea, black tea, and oolong tea; plants containing cocoa polyphenols such as cocoa; plants containing rutin such as buckwheat and buckwheat of the Polygonaceae family; plants containing curcumin such as turmeric; plants containing isoflavones from leguminous plants such as water oak, soybeans, bean sprouts, double bean sprouts, kudzu root, red clover and red clover sprouts; plants containing chlorogenic acids (coffee polyphenols) such as coffee; and plants containing other polyphenols such as ferulic acid, tannins, ellagic acid, lignans, or coumarins.

[0016] In particular, it is preferable to use plants containing anthocyanins, especially fruits containing anthocyanins, as the plants containing polyphenols. Among fruits containing anthocyanins, grapes are particularly preferred. That is, in this invention, it is preferable to use waste obtained from the use of grapes as the plant-derived waste containing polyphenols. When grapes are used as a raw material for fruit juice beverages or wine, the skins and other parts containing anthocyanins become waste. In addition, waste from wine production may include the stems and seeds after destemming. Furthermore, in wine production, the sediment that settles at the bottom of the tank becomes waste.

[0017] In the present invention, although not particularly limited, it is preferable to use waste products from wine production. Specifically, as plant-derived waste products containing polyphenols, it is preferable to use pomace and / or lees after grape pressing.

[0018] Furthermore, in the present invention, the extract of plant-derived waste containing polyphenols can refer to a solution obtained by immersing the residual components (presses) remaining after pressing a plant containing polyphenols, the residual components after distillation of a plant containing polyphenols, and the residual components after solvent extraction of a plant containing polyphenols in a solvent, and extracting the polyphenols contained in the residual components. Examples of solvents for extracting polyphenol components include water and aqueous organic solvents. For example, in wine production, the presses remaining after grape pressing or the sediment settled at the bottom of a wine tank can be immersed in a suitable solvent, and a solution obtained by extracting the polyphenol components contained in the presses or sediment can be used.

[0019] While not particularly limited, grape varieties used in wine production include, for example, white wine grape varieties such as Chardonnay, Semillon, Riesling, Sauvignon Blanc, Müller-Thurgau, Muscat, Verdelé, Koshu, Traminer, Delaware, and Niagara. Red wine grape varieties include, for example, Cabernet Sauvignon, Cabernet Franc, Merlot, Pinot Noir, Syrah, Alicante, Nebbiolo, Muscat Bailey A, and Black Queen.

[0020] Furthermore, in this invention, the plant-derived waste containing polyphenols is not limited to the grape-derived waste mentioned above, but can also be used from plants belonging to the citrus genus (Citrus) of the Rutaceae family, such as lime, lemon, grapefruit, summer orange, iyokan, unshu mandarin, and ponkan; the forum genus of the Rutaceae family, such as long-leaf kumquat and round kumquat; the grape genus of the Vitaceae family, such as grapes; the genus Vaccinium of the Ericaceae family, such as blueberries and cranberries; the genus Malus of the Rosaceae family, such as apples; and the genus Diospyros of the Ebenaceae family, such as persimmons.

[0021] Furthermore, the plant-derived waste containing polyphenols can consist of one type of plant-derived waste, or two or more types of plant-derived waste can be used. For example, a combination of grape pomace and coffee beans after extraction can be used as plant-derived waste containing polyphenols.

[0022] In the virus inactivation method according to the present invention, hydroxyl radicals generated by irradiating plant-derived waste containing polyphenols or an extract thereof with light are applied to viruses to inactivate them. Here, virus inactivation means the effect of removing or reducing the virus's ability to infect or reproduce. The viruses to be treated are not particularly limited, but include both enveloped and non-enveloped viruses. The viruses to be treated may be any of double-stranded DNA viruses, single-stranded DNA viruses, double-stranded RNA viruses, single-stranded (+) RNA viruses, single-stranded (-) RNA viruses, and reverse transcription viruses. Examples of viruses that can be inactivated include viruses belonging to the Caliciviridae, Orthomyxoviridae, Coronaviridae, and Herpesviridae families. Examples of viruses belonging to the Caliciviridae family include viruses belonging to the genera Norovirus, Sapovirus, Lagovirus, Nebovirus, and Vesivirus. Viruses belonging to the Orthomyxoviridae family include those belonging to the genera Influenza A, Influenza B, Influenza C, Togotovirus, and Isavirus. The Coronaviridae family includes the Retovirus subfamily and the Orthocoronavirus subfamily. Viruses belonging to the Retrovirus subfamily of the Coronaviridae family include those belonging to the Alpharetovirus genus. Viruses belonging to the Orthocoronavirus subfamily of the Coronaviridae family include those belonging to the Alphacoronavirus, Betacoronavirus, Deltacoronavirus, and Gammacoronavirus genera. Furthermore, viruses belonging to the Herpesviridae family include those belonging to the genera Simplevirus, Valisserovirus, Lymphocryptovirus, Cytomegalovirus, Roseolovirus, and Radinovirus.

[0023] The light used to irradiate plant-derived waste containing polyphenols or its extract can be arbitrarily determined from the wavelength range of visible light of 360 nm to 830 nm. To provide higher energy, it is preferable to use visible light with a shorter wavelength. For example, it can be visible light of 360 to 450 nm, preferably visible light of 360 to 400 nm, and more preferably visible light of 360 to 380 nm.

[0024] Also, the light used to irradiate plant-derived waste containing polyphenols or its extract may have an irradiance and irradiation time such that hydroxyl radicals can be generated from the waste or its extract. As an example, the irradiance can be 10 to 1000 [mW / cm 2 for 2 to 30 [seconds], preferably the irradiance is 50 to 100 [mW / cm 2 for 2 to 30 [seconds], and more preferably the irradiance is 50 to 100 [mW / cm 2 for 2 to 15 [seconds]. Also, the irradiance can be 100 [mW / cm 2 for 15 to 30 [seconds], or the irradiance can be 50 [mW / cm 2 for 2 to 6 [seconds]. By setting the light irradiation conditions within this range, sufficient hydroxyl radicals for virus inactivation can be rapidly generated.

[0025] As described above, according to the virus inactivation method of the present invention, hydroxyl radicals are generated by irradiating light on plant-derived waste containing polyphenols or its extract, thereby inactivating the virus. The inactivation of the virus can be quantitatively evaluated, for example, by the TCID 50 (Median tissue culture infectious dose, 50% infectious dose) method or the plaque method. According to the virus inactivation method of the present invention, TCID 50When the viral load (infectious viral load) is measured by law, it can be statistically significantly reduced compared to the untreated virus, and more specifically, the viral load can be reduced by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%.

[0026] The virus inactivation method according to the present invention, as described above, can be realized, for example, as a virus inactivation device 1 as shown in Figure 1. The virus inactivation device 1 shown in Figure 1 comprises a waste holding unit 2 (radical generation unit) containing plant-derived waste or an extract thereof containing polyphenols, and a light irradiation unit 3 that irradiates the plant-derived waste or an extract thereof held in the waste holding unit 2 with light of a predetermined wavelength and irradiance.

[0027] In the virus inactivation device 1, the waste holding section 2 can be made of a frame filled with the aforementioned plant-derived waste, or a nonwoven or woven fabric impregnated with an extract of the plant-derived waste. Furthermore, it is preferable that the waste holding section 2 in the virus inactivation device 1 is detachably arranged.

[0028] In the virus inactivation device 1 configured in this way, light of a predetermined wavelength and irradiance is irradiated from the light irradiation unit 3 onto the waste holding unit 2. As a result, hydroxyl radicals are generated in the space 4 between the waste holding unit 2 and the light irradiation unit 3 from polyphenol components contained in plant-derived waste or its extract. Therefore, by introducing a gas containing viruses into this space 4, the viruses can be inactivated in the space 4.

[0029] Furthermore, if the waste holding unit 2 in the virus inactivation device 1 is detachable, the waste holding unit 2 can be replaced when the amount of hydroxyl radicals generated decreases. This prevents a decrease in the amount of hydroxyl radicals generated and allows for the continuous treatment of virus-containing gases.

[0030] On the other hand, the virus inactivation device 1 to which the present invention is applied is not limited to the configuration shown in Figure 1, and as shown in Figure 2, it may also be equipped with a spraying device 5 that sprays plant-derived waste containing polyphenols or an extract thereof, instead of the waste holding unit 2. In the virus inactivation device 1, the spraying device 5 comprises a tank 6 filled with a solution containing plant-derived waste or an extract solution of plant-derived waste, and a spray nozzle 8 connected to the tank 6 via a pipe member 7. The tank 6 can be additionally filled with a solution containing plant-derived waste or an extract solution of plant-derived waste.

[0031] In the virus inactivation device 1 configured in this way, an extract of plant-derived waste can be sprayed from the spraying device 5. The virus inactivation device 1 then irradiates the sprayed plant-derived waste extract with light of a predetermined wavelength and irradiance from the light irradiation unit 3. As a result, hydroxyl radicals are generated from the polyphenol components contained in the sprayed extract in the space 4 between the waste holding unit 2 and the light irradiation unit 3. Therefore, by introducing a gas containing viruses into this space 4, the viruses can be inactivated in the space 4.

[0032] According to the virus inactivation device 1 shown in Figure 2, a solution containing plant-derived waste or an extract of plant-derived waste is sprayed by the spraying device 5, allowing for the generation of hydroxyl radicals over a relatively wide area (space 4), and thus enabling the treatment of virus-containing gas over a wide area. Furthermore, with the virus inactivation device 1 shown in Figure 2, the solution containing plant-derived waste or an extract of plant-derived waste can be additionally filled into the tank 6, allowing for the treatment of virus-containing gas over a long period of time.

[0033] As described above, the virus inactivation device 1 to which the present invention is applied can inactivate viruses contained in gases with hydroxyl radicals generated from plant-derived waste containing polyphenols or extracts thereof. Therefore, by applying the virus inactivation device 1 to a ventilation device, viruses in the air can be inactivated. For example, as shown in Figure 3, a ventilation device 10 equipped with the virus inactivation device 1 can be configured in which the virus inactivation device 1 is placed in the middle of a ventilation passage 13 that has an intake port 11 for taking in ambient air and an exhaust port 12 for discharging the treated air. Here, the ventilation device 10 is not limited in any way by the size of the device or other components, and includes any device that passes air through the ventilation passage 13 and supplies air from the exhaust port 12. Examples of ventilation devices 10 include air conditioners that supply air set to a desired temperature, air purifiers that remove airborne particles, hair dryers, dryers, and blowers that circulate air within the device. Therefore, although not shown in the figures, the ventilation device 10 may be equipped with a fan, motor, temperature control device (cooling device or heater), filter, etc., as means for blowing air.

[0034] In the ventilation device 10 configured in this way, air introduced from the intake port 11 is exposed to hydroxyl radicals within the virus inactivation device 1, thereby inactivating viruses contained in the air. Therefore, the ventilation device 10 can supply virus-inactivated air from the exhaust port 12. More specifically, as shown in Figure 4, the ventilation device 10 can be equipped with the virus inactivation device 1 shown in Figure 2. In this case, in space 4, light is irradiated under predetermined conditions onto an extract of plant-derived waste in mist form, and hydroxyl radicals generated as a result are present. The air introduced from the intake port 11 is then exposed to hydroxyl radicals in space 4. The virus-inactivated air is then discharged from the exhaust port 12.

[0035] In this case, the ventilation device 10 may also be equipped with a filter 14 between the virus inactivation device 1 and the exhaust port 12, as shown in Figure 5. The filter 14 can be, for example, a frame filled with the plant-derived waste described above, or a nonwoven or woven fabric impregnated with an extract of the plant-derived waste. If the filter 14 contains the plant-derived waste containing the polyphenols described above or an extract thereof, it can eliminate residual hydroxyl radicals.

[0036] Incidentally, the virus inactivation device 1 described above can be used not only in the ventilation device 10, but also in the decontamination device 20 as shown in Figure 6. Here, the decontamination device 20 is used to decontaminate any object 21 that may be contaminated by viruses, such as masks, medical goggles, and clothing of medical personnel. The decontamination device 20 comprises a virus inactivation device 1 having a spraying device 5 and a light irradiation unit 3 that irradiates light onto an extraction solution of plant-derived waste sprayed into the space 4, and a platform 22 on which the object to be treated is placed.

[0037] As described above, the decontamination device 20 can decontaminate the object to be treated 21 placed on the mounting platform 22 by hydroxyl radicals generated in the space 4. In other words, the virus inactivation device 1 can not only inactivate viruses in the air but also inactivate viruses attached to the object to be treated. At this time, the polyphenol components contained in the sprayed plant-derived waste extract solution have antioxidant properties, unlike hydroxyl radicals, and therefore can add functionality to the object to be treated due to the antioxidant properties of the polyphenol components. [Examples]

[0038] The present invention will be described in more detail below using examples, but the technical scope of the present invention is not limited to these examples.

[0039] [Example 1] In this example, based on the finding that hydroxyl radicals are generated via hydrogen peroxide when polyphenols are irradiated with light under predetermined conditions (Tsukada M, Nakashima T, Kamachi T, Niwano Y. Prooxidative potential of photo-irradiated aqueous extracts of grape pomace, a recyclable resource from winemaking. PLoS One, 11(6):e0158197, 2016), we investigated whether viruses could be inactivated by the hydroxyl radicals generated in this way.

[0040] <Experimental Method> In this example, influenza virus and norovirus were used as the treatment targets. Influenza virus was used to infect MDCK cells (derived from canine kidney cells), and norovirus was used to infect RAW264.7 cells (derived from mouse macrophages), and both were cultured. In this example, the viral load was 3 × 10⁶. 5 The volume was set to 100 μL / well.

[0041] A polyphenol-containing sample and a virus solution were mixed in a 1:10 ratio, irradiated with ultraviolet light (405 nm, 20 W) for 0, 15, 30, and 120 seconds, and then subjected to TCID. 50 The amount of virus reduction was quantified using the method. In this example, the polyphenol-containing samples used were grape skin water extract (polyphenol content: 760 μg / mL) and persimmon bud water extract (polyphenol content: 390 μg / mL), and water was used as the control solvent.

[0042] <Result> The results of this example showed that the most effective inactivation was achieved with a UV light irradiation time of 15 seconds, and the persimmon calyx water extract was used to inactivate influenza virus and norovirus, respectively. 50 / mL=10 4 / mL (a reduction of 99.9%) and TCID 50 / mL=10 2 / mL (a decrease of 90%), and TCID was obtained with grape skin water extract.50 / mL=10 3 / mL (99% reduction rate) and TCID 50 / mL=10 2 The result was / mL (a decrease of 90%).

[0043] These results indicate that, regardless of the type of polyphenol, light irradiation can be used to inactivate viruses.

[0044] [Example 2] In this example, the amount of hydrogen peroxide generated when the grape skin extract and persimmon bud extract used in Example 1 were irradiated with light was measured.

[0045] <Experimental Method> In Example 1, the effectiveness of the virus inactivation test was obtained for grape skin water extract and persimmon bud water extract. Therefore, the conditions of 15 seconds and 30 seconds of light irradiation were investigated. First, each sample was prepared as a 5-fold and 10-fold dilution of the original concentration, and irradiated with light (402 nm, 20 W) for the specified times. After irradiation, the amount of hydrogen peroxide was quantified using the principle commonly used for measuring hydrogen peroxide levels (quantification based on the formation of a complex of xylinol orange and ferric (produced by the oxidation of peroxide-dependent divalent iron ions)). A 2-fold dilution series of 10 μM hydrogen peroxide solutions was used as the calibration curve standard.

[0046] <Result> The measurement results are shown in Table 1.

[0047] [Table 1]

[0048] As shown in Table 1, the results of Example 1 showed that the persimmon-derived polyphenol-containing extract tended to have higher inactivating activity than the grape-derived polyphenol-containing extract, and the test results of this example supported this finding. Furthermore, while gallic acid (a single polyphenol) tends to lose its efficacy with prolonged irradiation, it was considered that the generation of hydroxyl radicals over a long period of time is possible when using plant-derived extracts that are thought to contain multiple types of polyphenols.

Claims

1. A virus inactivation method comprising the steps of: irradiating plant-derived waste containing polyphenols or an extract thereof with light to generate hydroxyl radicals; and inactivating viruses contained in the target to be treated with the generated hydroxyl radicals.

2. 2. The virus inactivation method according to claim 1, wherein the plant-derived waste is waste from wine production.

3. 3. The virus inactivation method according to claim 2, wherein the waste material from wine production is press pomace and / or lees remaining after grape pressing.

4. The plant-derived waste or its extract is irradiated with light at a rate of 10 to 1000 mW / cm 2 2. The method for inactivating viruses according to claim 1, wherein the heating time is 2 to 30 seconds.

5. 2. The method for inactivating viruses according to claim 1, wherein a solution containing plant-derived waste or an extract thereof is sprayed and the atomized solution is irradiated with light.

6. a radical generating unit that includes a plant-derived waste or an extract thereof containing polyphenols and generates hydroxyl radicals from the plant-derived waste or the extract thereof; a light irradiating unit that irradiates the plant-derived waste or the extract thereof of the radical generating unit with light, A virus inactivation device that generates hydroxyl radicals by irradiating light from a light irradiation unit onto the plant-derived waste or an extract thereof in the radical generation unit, and inactivates viruses contained in the treatment target using the generated hydroxyl radicals.

7. 7. The virus inactivation apparatus according to claim 6, wherein the plant-derived waste is waste from wine production.

8. 8. The virus inactivation device according to claim 7, wherein the waste material in wine production is press pomace and / or lees remaining after grape pressing.

9. The light irradiation unit irradiates the plant-derived waste or its extract with light at a rate of 10 to 1000 mW / cm 2 7. The virus inactivation device according to claim 6, wherein the time is set to 2 to 30 seconds.

10. the radical generating unit includes a spraying device that sprays a solution containing plant-derived waste or an extract thereof, 7. The virus inactivation device according to claim 6, wherein the light irradiating unit irradiates the atomized solution sprayed from the spray device with light.

11. 11. A ventilation device comprising: a ventilation duct having an intake port and an exhaust port; and the virus inactivation device according to claim 6, wherein air that has come into contact with hydroxyl radicals generated in the virus inactivation device is exhausted from the exhaust port in the ventilation duct.

12. 12. The ventilation device according to claim 11, wherein the radical generating section in the virus inactivation device is detachably disposed.

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