Plant activators

Abstract

To provide a plant activator that resists being washed off.SOLUTION: A plant activator contains at least one compound selected from an oxo fatty acid or a derivative thereof or a salt thereof and a hydroxylated fatty acid or a derivative thereof or a salt thereof, and an unsaturated fatty acid (excluding the oxo fatty acid and hydroxylated fatty acid).SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a plant activator. [Background technology]

[0002] Technologies for regulating plant growth have been developed with the aim of improving the supply efficiency of grain plants and horticultural plants. In addition to measures such as optimizing temperature and sunlight conditions and fertilization, methods for activating plants using plant activators that have plant growth regulating effects such as growth promotion, dormancy suppression, and stress suppression have been reported.

[0003] Patent Document 1 reports a plant activator that is characterized by containing an oxo fatty acid derivative or its salt or ester as an active ingredient. The plant activator described in Patent Document 1 has excellent resistance-inducing and growth-promoting effects in plants, but the use of this activator to induce plant resistance and / or promote plant growth has a problem in that the effect of the activator may not last long enough after application to the plant.

[0004] From the viewpoint of labor saving in agricultural work, etc., the durability of the effect of the activating component of the plant activator is an issue. It is presumed that the insufficient durability of the effect of the plant activator described in Patent Document 1 is due to the possibility that the activator does not remain in the plant body at a sufficient concentration because it is washed off from the surface of the plant to which it is applied. However, there is no effective means for making the activator remain, and there has been a strong demand for a technology for obtaining a satisfactory performance in terms of the durability of the effect of the activating component. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2018 / 168860 Summary of the Invention [Problem to be solved by the invention]

[0006] Therefore, an object of the present invention is to provide a plant activator which has improved durability of effect, has a long residual effect in a plant body to which it is applied, and has excellent disease resistance inducing effect and growth promoting effect. [Means for solving the problem]

[0007] The present invention relates to a plant activator containing at least one compound selected from an oxo fatty acid, a derivative thereof, or a salt thereof, and a hydroxylated fatty acid, a derivative thereof, or a salt thereof, and an unsaturated fatty acid (excluding oxo fatty acid and hydroxylated fatty acid).

[0008] The plant activator is preferably an unsaturated fatty acid having 10 to 20 carbon atoms.

[0009] The plant activator is preferably one in which the unsaturated fatty acid is selected from the group consisting of mono- or polyunsaturated fatty acids such as oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid.

[0010] The plant activator is preferably one in which the unsaturated fatty acid is linoleic acid.

[0011] The oxo fatty acid or a derivative or a salt thereof has the following formula: HOOC-(R 1 )-CH=CH-C(=O)-R 2 (I) (In the formula, R 1 : a linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms, R 2 : an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and / or double bonds) A preferred plant activator is an oxo fatty acid having the structural formula:

[0012] The oxo fatty acid is R 1The hydrocarbon group has 8 to 10 carbon atoms, and R 2 The plant activator which is an oxo fatty acid having an alkyl group with 4 to 6 carbon atoms is preferred.

[0013] The oxo fatty acid is R 1 Preferred are plant enhancers which are oxo fatty acids containing a double bond which forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I).

[0014] The oxo fatty acid is R 1 is a linear or branched hydrocarbon group having 9 carbon atoms, R 2 However, a plant activator that is an oxo fatty acid, which is an alkyl group having 5 carbon atoms, is preferred.

[0015] The plant activator is preferably such that the oxo fatty acid is ketooctadecadienoic acid.

[0016] The plant activator is preferably one in which the oxo fatty acid is 13-oxo-9,11-octadecadienoic acid.

[0017] The hydroxylated fatty acid or its derivative or salt thereof is represented by the following formula (II) and / or (III): HOOC-(R 3 )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R 4 (II) HOOC-(R 3 )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R 4 (III) (In the formula, R 3 is a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and / or OH groups, and when it contains a double bond, the position of the double bond is not limited; R 4is a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and / or OH groups, and when it contains a double bond, the position of the double bond is not limited. A preferred plant activator is a hydroxylated fatty acid having the structural formula:

[0018] The hydroxylated fatty acid is R 3 The hydrocarbon group has 6 to 8 carbon atoms, and R 4 A plant activator in which the hydrocarbon group has 4 to 6 carbon atoms is preferred.

[0019] The hydroxylated fatty acid is R 3 But -(CH 2 ) n -(n is an integer from 4 to 12), 4 But, C n H 2n+1 A plant activator having the structure -(n is an integer of 2 to 8) is preferred.

[0020] The hydroxylated fatty acid is R 3 is an alkylene group with 7 carbon atoms (-(CH 2 ) 7 -) and R 4 is an alkyl group with 5 carbon atoms (CH 3 CH 2 CH 2 CH 2 CH 2 -) plant activators are preferred.

[0021] The plant activator is preferably such that the hydroxylated fatty acid is hydroxyoctadecenoic acid.

[0022] The plant activator is preferably such that the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecenoic acid.

[0023] The plant activator is preferably such that the hydroxylated fatty acid is 9,12,13-trihydroxy-10-octadecenoic acid.

[0024] In addition, "octadecaenoic acid" is a conventional notation (for example, JP-A-3-14539, etc.), and the above-mentioned "9,10,13-trihydroxy-11-octadecenoic acid" is also written as "9,10,13-trihydroxyoctadec-11-enoic acid" or "9,10,13-trihydroxy-11-octadecenoic acid". Similarly, the above-mentioned "9,12,13-trihydroxy-10-octadecenoic acid" is also written as "9,12,13-trihydroxyoctadec-10-enoic acid" or "9,12,13-trihydroxy-10-octadecenoic acid". In addition, in the examples, the manufacturer's name is also written in parentheses. In addition, the above explanation applies to all "octadecaenoic acid" used in this specification, claims, drawings and abstract.

[0025] The structural formula of “9,10,13-trihydroxy-11-octadecenoic acid” is shown in the following structural formula (1).

[0026] [ka]

[0027] The structural formula of “9,12,13-trihydroxy-10-octadecenoic acid” is shown in the following structural formula (2).

[0028] [ka]

[0029] It is preferable that the plant activator contains the unsaturated fatty acid such that the ratio of the at least one compound selected from the oxo fatty acid or its derivative or salt thereof and the hydroxylated fatty acid or its derivative or salt thereof to the unsaturated fatty acid is 10% to 90% by weight.

[0030] The derivatives of the oxo fatty acids and hydroxylated fatty acids are preferably esters of oxo fatty acids and hydroxylated fatty acids, respectively. As salts of the oxo fatty acids and hydroxylated fatty acids, salts such as sodium salts, potassium salts, and ammonium salts, which will be described later, can be used.

[0031] The plant activator is preferably a plant activator used as a spray or immersion agent to be brought into contact with the stems, leaves or roots of a plant, or as a soil drenching agent.

[0032] The plant activator is preferably a plant activator used for plants selected from the Brassicaceae, Poaceae, Leguminosae, Solanaceae, Rosaceae, Amaranthaceae, and Malvaceae families. Effect of the Invention

[0033] The plant activator of the present invention has excellent disease resistance inducing effects and growth promoting effects, and since it is effectively prevented from being washed off from the plant body, these effects are highly persistent. [Brief description of the drawings]

[0034] [Figure 1] 1 is a graph showing the effect of the coexistence of unsaturated fatty acids on resistance to washing off. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0035] Plant activators The plant activator of the present invention is characterized by containing at least one compound selected from an oxo fatty acid or a derivative or a salt thereof and a hydroxylated fatty acid or a derivative or a salt thereof, and an unsaturated fatty acid (excluding oxo fatty acid and hydroxylated fatty acid).

[0036] In the present invention, "plant activation" means adjusting the growth activity of a plant in some way to activate or maintain it, and is a concept that includes plant growth regulating actions such as growth promotion (a concept that includes the expansion of stems and leaves, and the promotion of tuber and root growth, etc.), dormancy suppression, induction or conferring of resistance to plant stress (e.g., disease, etc.), and anti-aging.

[0037] The plant activator of the present invention contains at least one compound selected from oxo fatty acid or its derivative or its salt and hydroxylated fatty acid or its derivative or its salt as an active ingredient for exhibiting such a plant activating effect. By containing at least one compound selected from such a compound or a derivative or its salt, the plant activator of the present invention can impart a growth promoting effect to a plant by applying it to a part of a stem, leaf or root of a plant. In a plant body inoculated with the plant activator of the present invention, an increase in leaf length and leaf weight, which are growth indicators of a plant, and promotion of tuber or tuberous root growth are confirmed compared to an untreated plant, so that the plant activator of the present invention is considered to impart a growth promoting effect to a plant. By using the plant activator of the present invention, the growth of the plant body can be promoted, and the yield of the plant body such as vegetables, grains, and fruits can be increased. The plant growth promoting effect of the plant activator of the present invention is very high, and as a result, it can bring about an excellent yield increase effect and improved harvesting efficiency of commercial crops. Furthermore, by applying the plant activator of the present invention to a part of the stems, leaves, or roots of a plant, the salicylic acid pathway involved in resistance induction in the plant body can be activated, and as a result, resistance to diseases and the like can be induced in the plant.

[0038] However, as described above, although the plant activator containing an oxo fatty acid derivative or its salt or ester as an active ingredient has an excellent activating effect, the active ingredient may be washed away from the site of action by, for example, rain before or while the active ingredient can produce a sufficient effect. In many applications including local application of the plant activator, such washing away of the active ingredient in the plant activator by rain, wind erosion and other erosive forces may significantly reduce the efficacy and duration of action of the plant activator.

[0039] The plant activator of the present invention further contains an unsaturated fatty acid in addition to the above-mentioned active ingredient, that is, at least one compound selected from oxo fatty acid or its derivative or its salt and hydroxylated fatty acid or its derivative or its salt. The unsaturated fatty acid of the present invention does not include oxo fatty acid and hydroxylated fatty acid. The unsaturated fatty acid contained in the plant activator of the present invention is hydrophobic and has low water solubility. As a result, the unsaturated fatty acid of the present invention can impart the plant activator with a water-repellent property at the inoculation site. That is, the plant activator of the present invention is hardly washed off. Furthermore, the active ingredient, oxo fatty acid or its derivative or its salt and / or hydroxylated fatty acid or its derivative or its salt, exists so as to be included in the unsaturated fatty acid coexisting in the plant activator. Therefore, the plant activator of the present invention shows remarkable resistance to being washed off by rain, etc., and the active ingredient adheres more strongly to the surface of the plant body such as the leaves of the plant, and as a result, the duration of the activating effect of the plant activator is increased.

[0040] The oxo fatty acid or a derivative or a salt thereof of the present invention is represented by the following formula: HOOC-(R 1 )-CH=CH-C(=O)-R 2 (I) (In the formula, R 1 A linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms. R 2: an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and / or double bonds) An oxo fatty acid having the structural formula: or a derivative or salt thereof can be suitably used.

[0041] In one embodiment of the present invention, R in the oxo fatty acid 1 The hydrocarbon group has 8 to 10 carbon atoms, and R 2 The number of carbon atoms in the alkyl group is 4 to 6. In another embodiment, R 1 contains a double bond that forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I). 1 is a linear or branched hydrocarbon group having 9 carbon atoms, and R 2 is preferably an alkyl group having 5 carbon atoms.

[0042] The oxo fatty acid of the present invention specifically includes ketooctadecadienoic acid. For example, ketooctadecadienoic acid includes, but is not limited to, 9-oxo-10,12-octadecadienoic acid (9-oxoODA), 13-oxo-9,11-octadecadienoic acid (13-oxoODA), 5-oxo-6,8-octadecadienoic acid, 6-oxo-9,12-octadecadienoic acid, 8-oxo-9,12-octadecadienoic acid, 10-oxo-8,12-octadecadienoic acid, 11-oxo-9,12-octadecadienoic acid, 12-oxo-9,13-octadecadienoic acid, and 14-oxo-9,12-octadecadienoic acid and their isomers.

[0043] In addition, esters are preferred as derivatives of oxo fatty acids. Examples of esters of oxo fatty acids of the present invention include, but are not limited to, methyl esters, ethyl esters, propyl esters, butyl esters, pentyl esters, isopentyl esters, octyl esters, etc. In addition, examples of salts of oxo fatty acids include ammonium salts such as ammonium salts and alkyl ammonium salts such as tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, alkali metal salts such as sodium salts, lithium salts, potassium salts, and metal salts such as cobalt salts and manganese salts, but are not particularly limited as long as they are one or more agriculturally acceptable salts, such as salts contained in fertilizers.

[0044] The hydroxylated fatty acid or its derivative or salt thereof of the present invention is represented by the following formula (II) and / or (III): HOOC-(R 3 )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R 4 (II) HOOC-(R 3 )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R 4 (III) (In the formula, R 3 is a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and / or OH groups, and when it contains a double bond, the position of the double bond is not limited; R 4 is a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and / or OH groups, and when it contains a double bond, the position of the double bond is not limited. A hydroxylated fatty acid having the structural formula: or a derivative or salt thereof can be suitably used.

[0045] In one embodiment of the present invention, R in the hydroxylated fatty acid 3 The hydrocarbon group has 6 to 8 carbon atoms, and R4 In another embodiment, the hydrocarbon group of R in the hydroxylated fatty acid has 4 to 6 carbon atoms. 3 is -(CH 2 ) n -(n is an integer from 4 to 12), 4 is C n H 2n+1 -(n is an integer of 2 to 8). 3 is an alkylene group with 7 carbon atoms (-(CH 2 ) 7 -) and R 4 is an alkyl group with 5 carbon atoms (CH 3 CH 2 CH 2 CH 2 CH 2 -) is preferred.

[0046] The hydroxylated fatty acids of the present invention specifically include hydroxyoctadecenoic acids, such as, but not limited to, 9,10,13-trihydroxy-11-octadecenoic acid and / or 9,12,13-trihydroxy-10-octadecenoic acid and their isomers.

[0047] In addition, as the derivative of hydroxylated fatty acid, ester is preferable. Examples of ester of hydroxylated fatty acid of the present invention include, but are not limited to, methyl ester, ethyl ester, propyl ester, butyl ester, pentyl ester, isopentyl ester, octyl ester, etc. In addition, examples of salt of hydroxylated fatty acid include ammonium salt such as alkyl ammonium salt such as ammonium salt, tetramethylammonium salt, etc., alkaline earth metal salt such as calcium salt, magnesium salt, etc., alkali metal salt such as sodium salt, lithium salt, potassium salt, cobalt salt, manganese salt, etc., but are not particularly limited as long as it is one or more kinds of salts that are agriculturally acceptable, such as salts contained in fertilizers, etc.

[0048] The unsaturated fatty acid of the present invention is not particularly limited, but an unsaturated fatty acid having about 10 to 20 carbon atoms may be preferable from the viewpoint of low solubility in water. For example, an unsaturated fatty acid having 18 carbon atoms is more preferable. In addition, the number of unsaturated bonds in the molecular structure of the unsaturated fatty acid of the present invention is not limited, and it may be a monounsaturated fatty acid or a polyunsaturated fatty acid. For example, the unsaturated fatty acid may be, but is not limited to, unsaturated fatty acids such as oleic acid, linoleic acid, oleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. In addition, the unsaturated fatty acid may be in the form of either a free fatty acid or a salt thereof (a salt that can be generally used, for example, the salt exemplified above), and one or more of these may be selected. As the unsaturated fatty acid of the present invention, a commercially available product may be used, or it may be a synthetic product from a commercially available compound. In addition, as the unsaturated fatty acid, one type of unsaturated fatty acid may be used, or two or more types may be used.

[0049] In addition, when isomers exist in the compounds exemplified in this specification, all possible isomers can be used in the present invention, unless otherwise specified.

[0050] The plant activator of the present invention contains at least one compound selected from at least one oxo fatty acid or its derivative or its salt and at least one hydroxylated fatty acid or its derivative or its salt, and at least one unsaturated fatty acid. That is, the unsaturated fatty acid that can be used in the present invention is not limited to compatibility with any particular type of oxo fatty acid or its derivative or its salt, or hydroxylated fatty acid or its derivative or its salt, and a wide range of unsaturated fatty acids can be used in the plant activator of the present invention together with the oxo fatty acid or its derivative or its salt, or the hydroxylated fatty acid or its derivative or its salt. For example, the plant activator of the present invention may contain at least one compound selected from at least one oxo fatty acid selected from 13-oxoODA and 9-oxoODA, or a derivative or a salt thereof, and at least one hydroxylated fatty acid selected from 9,10,13-trihydroxy-11-octadecenoic acid and / or 9,12,13-trihydroxy-10-octadecenoic acid, or a derivative or a salt thereof, and at least one unsaturated fatty acid selected from oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. For example, the plant activator of the present invention may contain at least one compound selected from ketooctadecadienoic acid, or a derivative or a salt thereof, and hydroxyoctadecenoic acid, or a derivative or a salt thereof, and at least one unsaturated fatty acid selected from oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. For example, the plant activator of the present invention may contain 13-oxo-9,11-octadecadienoic acid, or a derivative or a salt thereof, and linoleic acid; it may contain 9,10,13-trihydroxy-11-octadecaenoic acid and / or 9,12,13-trihydroxy-10-octadecenoic acid, or a derivative or a salt thereof, and linoleic acid; or it may contain 13-oxo-9,11-octadecadienoic acid and / or 9,10,13-trihydroxy-11-octadecaenoic acid and / or 9,12,13-trihydroxy-10-octadecenoic acid, or a derivative or a salt thereof, and linoleic acid.

[0051] Since the plant activator of the present invention contains unsaturated fatty acids, washing off of the plant activator from the plant after inoculation is minimized. Moreover, in the plant activator of the present invention, the coexistence of unsaturated fatty acids does not reduce or destroy the excellent disease resistance induction effect and growth promotion effect of the active ingredient for the activating effect, i.e., at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts. In addition, the unsaturated fatty acids do not have adverse effects on the plant body to which they are applied. As a result, the efficacy and life span of the plant activator can be enhanced. Therefore, the plant activator of the present invention can reduce the frequency of application of the plant activator to the plant body and also reduce the application amount of the plant activator.

[0052] As described above, the plant activator of the present invention is a plant activator containing at least one compound selected from naturally occurring oxo fatty acid or its derivative or its salt, hydroxylated fatty acid or its derivative or its salt, and natural unsaturated fatty acid. Therefore, the plant activator of the present invention is also excellent in that it has a low environmental impact.

[0053] In the plant activator of the present invention, the unsaturated fatty acid and the active ingredient for the activation effect, i.e., at least one compound selected from oxo fatty acid, its derivative or its salt, and hydroxylated fatty acid, its derivative or its salt, are preferably contained in an amount of about 90% or less by weight of at least one compound selected from oxo fatty acid, its derivative or its salt, and hydroxylated fatty acid, its derivative or its salt, relative to the unsaturated fatty acid. If the ratio of at least one compound selected from oxo fatty acid, its derivative or its salt, and hydroxylated fatty acid, its derivative or its salt, relative to the unsaturated fatty acid is greater than 90%, the content of hydrophobic components in the plant activator is low, and washing off may not be effectively prevented. In addition, the unsaturated fatty acid and the active ingredient for the activation effect, i.e., at least one compound selected from oxo fatty acid, its derivative or its salt, and hydroxylated fatty acid, its derivative or its salt, are preferably contained in an amount of about 10% or more by weight of at least one compound selected from oxo fatty acid, its derivative or its salt, and hydroxylated fatty acid, its derivative or its salt, relative to the unsaturated fatty acid. If the ratio of at least one compound selected from oxo fatty acid or its derivative or its salt and hydroxylated fatty acid or its derivative or its salt to unsaturated fatty acid is less than 10%, the amount of active ingredient for activation effect in the plant activator is small, and the plant activation effect may be insufficient. For example, the ratio of at least one compound selected from oxo fatty acid or its derivative or its salt and hydroxylated fatty acid or its derivative or its salt to unsaturated fatty acid in the plant activator is about 10% or more and about 90% or less by weight. By containing the active ingredient for activation effect and unsaturated fatty acid in this ratio in the plant activator, a good activation effect and resistance to washing off can be obtained.

[0054] The plant activator of the present invention may contain a diluent or carrier suitable for use as a plant activator, if necessary. These additive components are not particularly limited as long as they are agriculturally acceptable agents. In addition, other than the diluent and carrier, components that are usually used in agricultural chemical formulations may also be contained.

[0055] The stabilized plant activator of the present invention can be applied to plants by any method. For example, it can be used as a spray or immersion agent that is brought into contact with the stems, leaves, or roots of a plant, or as a soil drenching agent. The specific application method can be appropriately selected depending on the cultivated plant to be applied and the form of use, and examples thereof include ground liquid spraying, ground solid spraying, aerial liquid spraying, aerial solid spraying, liquid surface spraying, application within a facility, soil mixing application, soil drenching application, surface treatment such as coating treatment, seedling box application, single flower treatment, and plant base treatment. In the plant to which it is applied, the plant activator of the present invention imparts to the plant a plant growth promoting effect and resistance to stress such as disease. The plant activator of the present invention further has high resistance to washing off, and therefore may be particularly preferable in the application for treating the stems and leaves of cultivated plants among the above application methods.

[0056] The plant to which the plant activator of the present invention can be applied is not particularly limited, and can be used effectively for plants in general. For example, it can be suitably applied to plants of the Brassicaceae, Poaceae, Leguminosae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae families. In addition, the plants to which the plant activator is applied are not limited to wild-type plants, and may be, for example, mutants or transformants. In addition, the varieties of each plant are not particularly limited. EXAMPLES

[0057] The present invention will be described based on examples, but the present invention is not limited to only the examples.

[0058] <Preparation of plant activators> Example 1 As at least one compound selected from oxo fatty acid or its derivative or its salt and hydroxylated fatty acid or its derivative or its salt, 13-oxo-9,11-octadecadienoic acid (13-oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical Company, 100 μg / 100 μL ethanol solution), which is a ketooctadecadienoic acid, was used. In addition, linoleic acid (primary linoleic acid from Fujifilm Wako Pure Chemical Industries, Ltd.) was used as an unsaturated fatty acid. 5 μL (5 μg) of 13-oxoODA was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 1 (ratio of oxo fatty acid to linoleic acid = 10%). Example 2 As in Example 1, 13-oxoODA was used as the oxo fatty acid, and linoleic acid was used as the unsaturated fatty acid. 45 μL (45 μg) of 13-oxoODA was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 2 (ratio of oxo fatty acid to linoleic acid=90%). Example 3 As at least one compound selected from oxo fatty acids or derivatives thereof or salts thereof and hydroxylated fatty acids or derivatives thereof or salts thereof, hydroxyoctadecaenoic acids, such as 9,10,13-trihydroxy-11-octadecenoic acid (Larodan Fine Chemicals, 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (English: 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid), 200 mg / L ethanol solution), and 9,12,13-trihydroxy-10-octadecenoic acid (Larodan Fine Chemicals, 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (English: 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid), A mixture of 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (200mg / L ethanol solution) and 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200mg / L ethanol solution) in a ratio of 2:1 was used. First, 200μL (40μg) of 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200mg / L ethanol solution) was mixed with 100μL (20μg) of 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200mg / L ethanol solution) to prepare 300μL of hydroxylated fatty acid mixture. In addition, linoleic acid (primary linoleic acid from Fujifilm Wako Pure Chemical Industries, Ltd.) was used as an unsaturated fatty acid. 25μL of this hydroxylated fatty acid mixture (5μg of hydroxylated fatty acid mixture) was added to 50μg of linoleic acid and mixed. This was used as the test plant activator of Example 3 (ratio of hydroxylated fatty acid to linoleic acid = 10%). Example 4 As in Example 3, a 2:1 hydroxylated fatty acid mixture of 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid and 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid was used as the hydroxylated fatty acid, and linoleic acid was used as the unsaturated fatty acid. 225 μL of this hydroxylated fatty acid mixture (45 μg of hydroxylated fatty acid mixture) was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 3 (ratio of hydroxylated fatty acid to linoleic acid = 90%). Comparative Example 1 13-oxoODA (100 μg / 100 μL ethanol solution) of Example 1 was used as a test plant activator of Comparative Example 1 not containing linoleic acid. Comparative Example 2 The 200 μg / mL hydroxylated fatty acid mixture prepared in Example 3 was used as a test plant activator of Comparative Example 2, which did not contain linoleic acid.

[0059] <Evaluation method> The test plant activators prepared in the above Examples 1 to 4 and Comparative Examples 1 and 2 were tested and evaluated by the following methods.

[0060] <Water repellency test> A water repellency test was carried out on the test plant activators of Examples 1 to 4 and Comparative Examples 1 and 2 not containing unsaturated fatty acids. The water used in the water repellency test was ion-exchanged water. In this water repellency test, a polypropylene resin plate was first prepared, and a spinach leaf with a length of about 15 cm was fixed to the resin plate. 5 μL of each test plant activator was spread and applied with a spatula to a width of 1 cm in the length direction on the spinach leaf, and then dried to form an evaluation surface for each test plant activator. The resin plate on which the spinach leaf was fixed was fixed to a jig so that the inclination angle of the evaluation surface with respect to the horizontal was 24 degrees, and 50 μL of ion-exchanged water was dropped from a position 2.5 cm above each evaluation surface along the length of the spinach leaf so that it passed through the evaluation surface, and the time required for the water to flow down a section of 11.5 cm including the evaluation surface (1 cm) from the drop position on the spinach leaf was measured. The results are shown in FIG. 1.

[0061] As shown in Fig. 1, water ran down quickly when the evaluation surface included the test plant activator containing unsaturated fatty acid (Examples 1 to 4). In other words, it was found that the surface to which the test plant activator containing unsaturated fatty acid was applied had high water repellency.

[0062] Therefore, when a plant activator containing unsaturated fatty acids such that the ratio of oxo fatty acids or their derivatives or salts, or hydroxylated fatty acids or their derivatives or salts to the unsaturated fatty acids is 10% to 90% by weight is applied, the resistance of the plant activator in contact with a fluid (water) is suppressed. As a result, the plant activator applied to a plant body is resistant to being washed off by rain or the like that falls continuously after application. As a result, the plant activator of the present invention is less likely to wash off the active ingredient, and adheres to the surface of the plant body such as the leaves of the plant for a longer period, and therefore the duration of the plant activating effect can be increased.

[0063] From the above results, it is apparent that the plant activator of the present invention is resistant to washing off and has a high and sustained plant activating effect.

Claims

1. A plant activator comprising ketooctadecadienoic acid or a derivative or a salt thereof, and linoleic acid, A plant activator (not including cyclodextrin) containing linoleic acid such that the ratio of the ketooctadecadienoic acid or a derivative or a salt thereof to the linoleic acid is 10% to 90% by weight.

2. 2. The plant activator according to claim 1, wherein the ketooctadecadienoic acid or a derivative or a salt thereof has the following formula: P.S. 1 )-CH=CH-C(=O)-R 2 (I) (In the formula, R 1 : a linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms, R 2 : an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and / or double bonds) or a derivative or salt thereof.

3. 3. The plant activator according to claim 2, wherein the ketooctadecadienoic acid is R 1 The hydrocarbon group has 8 to 10 carbon atoms, and R 2 The alkyl group has 4 to 6 carbon atoms.

4. 4. The plant activator according to claim 3, wherein the ketooctadecadienoic acid is R 1 is a ketooctadecadienoic acid containing a double bond which forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I).

5. 5. The plant activator according to claim 4, wherein the ketooctadecadienoic acid is R 1 is a linear or branched hydrocarbon group having 9 carbon atoms; R 2 is ketooctadecadienoic acid, which is an alkyl group having 5 carbon atoms.

6. 2. The plant activator according to claim 1, wherein the ketooctadecadienoic acid is 13-oxo-9,11-octadecadienoic acid.

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