Skin beautifying method, composition kit, multi-component skin penetration method, and multi-component skin penetration system

Abstract

To achieve a sufficient rejuvenation effect to enhance the user's level of satisfaction.SOLUTION: Provided is a skin beautification method (excluding medical treatment) including: a step for applying a first composition containing one or more substances selected from a sirtuin gene activator, a growth factor agent, and a proteoglycan; and a step for applying a second composition containing one or more substances, other than the first composition, selected from the sirtuin gene activator, the growth factor agent, and the proteoglycan.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] This invention relates to a skin beautifying method for beautifying the skin, a multi-component skin penetration method, and a multi-component skin penetration system.

Background Art

[0002] Conventionally, as a skin beautifying composition that improves wrinkles and has excellent usability, a skin beautifying composition characterized by containing an acetic acid extract of onion bulbs and sodium bicarbonate, and a skin beautifying method of applying the same (excluding medical acts) have been proposed (see Patent Document 1). In this skin beautifying method, even with only the acetic acid extract of onion bulbs, improvement of wrinkles, moisturizing effect, and skin beautifying effect (improvement of texture) are recognized. However, it is said that by containing sodium bicarbonate together, a more excellent skin beautifying effect (especially improvement of wrinkles) can be recognized in a shorter period. Here, in the beauty industry, further effects are required, and an effect of looking younger is required. That is, in addition to improvement of wrinkles, moisturizing effect, and skin beautifying effect, a lifting effect that improves facial sagging and wrinkles is also required. However, the above-described beauty method did not achieve a lifting effect.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] In view of the above problems, an object of this invention is to obtain a sufficient effect of looking younger and improve the satisfaction of users.

Means for Solving the Problems

[0005] This invention is a searchuin gene activatorand Growth factor agent one side of Applying a first composition containing the searchin gene activator and the growth factor agent the other side of and applying a second composition containing and the search gene activator is any one or a plurality of those selected from NAD+, NMN, 5-deazafavin, and niacinamide, and the growth factor agent is any one or a plurality of those selected from EGF, FGF, PDGF, exosome, and human stem cell culture agent A skin beautifying method (excluding medical practices), characterized by the above.

Advantages of the Invention

[0006] The present invention can provide a skin beautifying method, a multi-component skin penetration method, and a multi-component skin penetration system that can obtain a sufficient youthful appearance effect.

Brief Description of the Drawings

[0007]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Embodiments for Carrying Out the Invention

[0008] This invention involves applying a first composition containing a first active ingredient and a second composition containing a second active ingredient, either separately or by mixing them all together, onto the skin. The first active ingredient and the second active ingredient are such that one is a growth factor agent such as a human stem cell culture agent, and the other is a sirtuin gene activator such as NMN. Also, as a method of application, a first penetrant composed of the first active ingredient and a second penetrant composed of the second active ingredient are each or collectively adjusted with an adjuster such as purified water to form a liquid for spraying (liquid for application), and can be applied. As a method of application, it can be applied using a penetration device that promotes penetration into the skin, or by applying with fingers, or by applying with a pack. The penetration device can be an appropriate device such as a spraying device for spraying, an iontophoresis device that allows beauty components ionized negatively by flowing a weak negative current towards the skin to penetrate into the skin, an electroporation device that applies an electric pulse to the skin to temporarily create fine holes in the cell membrane and allow beauty components to penetrate to the dermis, a roller-type penetration promoter with a plurality of fine needles provided on the circumferential surface of a cylindrical roller, etc. Here, the skin referred to means all the skin of the body, such as the facial skin, neck skin, décolletage skin, limb skin, chest skin, abdominal skin, back skin, genital skin, head skin, etc. Hereinafter, an embodiment of this invention will be described together with the drawings.

[0009] Figure 1 is a configuration diagram showing the system configuration of the multi-component skin penetration system 1. The multi-component skin penetration system 1 is composed of a first penetrant 2, a second penetrant 3, an adjuster 4, and a spraying device 5.

[0010] The first penetrant 2 is an agent composed of a first active ingredient that penetrates into the skin, and is composed of a liquid or powder. The second penetrant 3 is an agent composed of a second active ingredient that penetrates into the skin, and is composed of a liquid or powder.

[0011] In this example, one of the first penetrant 2 (first active ingredient) and the second penetrant 3 (second active ingredient) is a sirtuin gene activator such as NMN (NMN agent), and the other is a growth factor agent such as a human stem cell culture agent.

[0012] The sirtuin gene activator can be a NAD+ precursor, a NAD+ analog component, NAD (NAD+), niacinamide, or a plurality of these, and can be in the form of a powder or a liquid. The NAD+ precursor can be nicotinamide mononucleotide (hereinafter referred to as NMN). The NAD+ analog component can be deazafavin or 5-deazafavin. 5-deazafavin can be 5-deazafavin (TND1128) or 5-deazafavin (F420) or both. The sirtuin gene activator can be in the form of a powder or a liquid. In the case of a powder, it can be powdered by microgranulation processing or a freeze-drying method.

[0013] NMN is a powder or liquid of nicotinamide mononucleotide (NMN), and is collected from breast milk, edamame, broccoli, avocado, tomato, and other organisms. When NMN is in liquid form, it is prepared by extraction from the above-mentioned organisms. In the case of NMN powder, it is formed by granulation such as microgranulation processing.

[0014] The growth factor agent can be a growth factor, exosome, stem cell culture supernatant, or a plurality of these, and can be in the form of a powder or a liquid. The growth factor can be EGF (epidermal growth factor), FGF (fibroblast growth factor), PDGF (platelet-derived growth factor), IGF-1 (insulin-like growth factor), HGF (hepatocyte growth factor), KGF (keratinocyte growth factor), TGF-β (transforming growth factor), or a plurality of these. For FGF, any appropriate FGF family member can be used, and it can be any one or more of FGF1 to FGF23, preferably any one or more of FGF1 to FGF14, more preferably any one or more of FGF1 to FGF10, and even more preferably FGF1 or FGF2. The growth factor agent can be in the form of a liquid or a powder. In the case of a powder, it can be obtained by freeze-drying the liquid growth factor agent by a freeze-drying method.

[0015] The growth factor agent is derived from the culture supernatant of human stem cells. This human stem cell culture supernatant can be obtained by culturing human stem cells from appropriate sources such as umbilical cord-derived, umbilical cord blood-derived, adipose-derived, dental pulp-derived, etc., and can be made into a liquid or powder.

[0016] In the culture of human stem cells, it is preferably subcultured up to a predetermined generation in subculture, and desirably up to 5 generations. Also, it is preferable to subculture while the growth curve during culture is linear. By doing so, it is possible to prevent the contamination of aging cells and prevent the contamination of the growth factor agent from aging cells.

[0017] The human stem cell culture agent is the supernatant obtained when stem cells collected from humans are cultured in a culture solution or a powder obtained by freeze-drying this supernatant by a freeze-drying method, and can be made into a human stem cell culture supernatant agent. The human stem cell culture supernatant agent can be a human umbilical cord stem cell culture supernatant obtained from a human umbilical cord, a human umbilical cord blood stem cell culture supernatant obtained from human umbilical cord blood, a human adipose stem cell culture supernatant obtained from human adipose tissue, a human bone marrow stem cell culture supernatant obtained from human bone marrow fluid, a supernatant obtained by culturing other human cells, or a supernatant powder obtained by powdering these supernatants by a freeze-drying method.

[0018] Both the first penetrant 2 and the second penetrant 3 are preferably 100% of the sirtuin gene activator or 100% of the growth factor agent, but other components may be contained in each. Note that all % notations in this specification are shown as mass percentages.

[0019] The sirtuin gene activator can be 10 ml or more, preferably 50 ml or more, more preferably 100 ml or more, still more preferably 150 ml or more, and even more preferably 200 ml or more. Also, the sirtuin gene activator can be 1000 ml or less, preferably 800 ml or less, more preferably 600 ml or less, and still more preferably 500 ml or less.

[0020] The growth factor agent can be 0.1 mg or more, preferably 0.5 mg or more, and more preferably 1 mg or more. Also, the growth factor agent can be 20 mg or less, preferably 15 mg or less, and more preferably 10 mg or less.

[0021] The regulator 4 can be purified water or physiological saline. As the purified water, ordinary purified water or sterilized purified water obtained by sterilizing purified water can be used. As the physiological saline, appropriate ones such as injection-grade physiological saline obtained by sterilizing purified water by filtration or distillation and further removing pyrogenic substances can be used.

[0022] The regulator 4 can be 0.5 ml or more, preferably 1 ml or more, and more preferably 2 ml or more. Also, the regulator 4 is preferably 20 ml or less, more preferably 15 ml or less, still more preferably 10 ml or less, and suitably 5 ml or less.

[0023] Note that the ratio of the growth factor agent, the sirtuin gene activator, and the regulator 4 can be within the ranges of the respective amounts described above. Therefore, the above amounts are sufficient for application centered on the face or the scalp, but for application to the whole body, etc., the amounts can be increased in a ratio within the above range.

[0024] The first penetrant 2 is adjusted by the adjuster 4 and used as the first spraying liquid (the first coating liquid). Similarly, the second penetrant 3 is adjusted by the adjuster 4 and used as the second spraying liquid (the second coating liquid). Note that the first penetrant 2 and the second penetrant 3 can be mixed and adjusted by the adjuster 4 to be used as one integrated spraying liquid (integrated coating liquid).

[0025] The spraying liquid (the first spraying liquid, the second spraying liquid, or the integrated spraying liquid) adjusted in this way can have a viscosity of 200 mPa·s or less, preferably 100 mPa·s or less, more preferably 50 mPa·s or less, even more preferably 10 mPa·s or less, still more preferably 5 mPa·s or less, even more preferably 3 mPa·s or less, still more preferably 2 mPa·s or less, and most preferably 1 mPa·s or less. Thereby, it can be prevented from clogging inside when spraying from the spray port 12a of the spraying device 5 described later.

[0026] The spraying device 5 is a device that sprays a pressurized liquid onto an object (human skin in this embodiment), and will be described in detail below.

[0027] The spraying device 5 includes a main body 30 that sends out air, a conduit 20 having one end connected to the main body 30, and a handpiece 10 connected to the other end of the conduit 20 and operated by an operator.

[0028] The handpiece 10 is a longitudinal unit that can be held by the operator's hand. A spray cover 11 is provided at the tip, and the other end of the conduit 20 is connected to the rear end. A liquid input section 15 for injecting a liquid for spraying, which is obtained by adjusting the first penetrant 2 or the second penetrant 3 with the regulator 4, is provided on the tip side from the center of the upper surface of the handpiece 10. The liquid input section 15 is provided with a liquid input hole 15b in the lid portion on the upper surface, and a liquid storage section 15a is provided inside the handpiece 10. This liquid storage section 15a is a sterile chamber, and the liquid for spraying is injected into the liquid storage section 15a from the liquid input hole 15b with a syringe or the like. On the rear end side from the center of the upper surface of the handpiece 10, there is provided a setting section 19 having a display section 19a (see FIG. 2) for displaying the current spray setting, a switching button 19b (see FIG. 2) for switching the spray setting of the liquid for spraying, an operation button 19c (see FIG. 2) for switching the start and stop of the spraying of the liquid for spraying, and a control section 19d for executing various controls. A dial-type spray amount adjustment section 18 for adjusting the spray amount of the liquid for spraying in one spraying is provided on the right side surface (front surface in FIG. 1) of the handpiece 10, and a manual spray button 16 for performing one spraying by pressing the spray setting when manual spraying is provided on the left side surface (rear surface in FIG. 1) of the handpiece 10.

[0029] The conduit 20 is provided inside with a pipe 20a (see FIG. 2) for supplying gas (air) to the handpiece 10 and an electric wire 20b (see FIG. 2) for supplying power to the handpiece 10. This conduit 20 can be freely bent, and the operator can freely move the handpiece 10 and use it for the treatment while leaving the main body 30 in place.

[0030] The main body 30 has an operation start button 30a for switching the start / stop of the operation provided on the front surface and a power cord (not shown) for connecting to a household power supply provided on the rear surface, and one end of the conduit 20 is connected to the side surface.

[0031] FIG. 2 is a block diagram showing the configuration of the multi-component skin penetration system 1. Inside the handpiece 10, a spray tip 12 is provided on the tip side, and a transparent and substantially cylindrical spray cover 11 is attached to the outer periphery of the spray tip 12. The spray cover 11 is cylindrical, with a shape where the tip is cut perpendicular to the axis of the cylinder, and gas vent spaces 11a are provided facing each other at two locations on the left and right at the tip. That is, a circular ring-shaped tip on the plane is mostly present, and at least a part of it is a concave portion that forms the gas vent space 11a. In the illustrated example, this gas vent space 11a is formed by a concave notch groove obtained by partially switching the end portion, which is the open portion of the spray cover 11.

[0032] At the center position of the spray tip 12 that is the tip of the handpiece 10, a spray port 12a for spraying the spray liquid is provided, and a gas release portion 14 is provided behind the spray port 12a. A spray gas chamber 14b is provided inside this gas release portion 14, and this spray gas chamber 14b communicates with the spray port 12a. The air port 14a at the tip of the spray gas chamber 14b and the spray port 12a are provided facing each other. A spray liquid chamber 13 is provided on the outer periphery between the air port 14a and the spray port 12a, and this spray liquid chamber 13 communicates with the spray port 12a. The spray liquid chamber 13 serves as a sterile chamber and communicates with a liquid storage portion 15a in which the spray liquid is stored. Also, the discharge rate of the spray liquid and gas discharged from the gas release portion 14 through the spray port 12a can be 100 km / h or more, preferably 500 km / h or more, and more preferably 1,000 km / h or more. Also, the upper limit of this discharge rate can be 5,000 km / h or less, preferably 3,000 km / h or less, and more preferably 2,000 km / h or less. By discharging the compressed gas at high speed in a short time in this way, the spray liquid can be atomized and discharged. By atomizing and spraying (coating) in this way, the spray liquid can penetrate deeper and more into the skin.

[0033] The spraying tip 12 having a spray port 12a has its outer periphery and the spray tip side covered by a spray cover 11. Therefore, the spraying liquid and gas sprayed from the spray port 12a are forcefully sprayed (injected) onto the skin to be sprayed while being confined within the spray cover 11, and the excess gas (including the spraying liquid) is discharged to the outside from the gas vent space 11a.

[0034] Inside the handpiece 10, a solenoid 17 is provided at the rear stage of the gas release portion 14. This solenoid 17 is provided in the middle of a pipe 20a that supplies gas to the spray gas chamber 14b of the gas release portion 14, and switches the passage / blockage of the gas in the pipe 20a. This solenoid 17 and a control unit 19d that controls it function as a spray control unit.

[0035] Also, a control unit 19d (see FIG. 1) is provided inside the handpiece 10. This control unit 19d, in response to the operation of the switching button 19b, switches the spray setting of the spraying liquid to any one of manual spraying where spraying is performed once when the manual spray button 16 is pressed, first intermittent spraying where instantaneous short-time spraying (for example, 0.1 seconds, etc.) is continuously performed at a slow first time interval (for example, 1.5 second intervals), second intermittent spraying where short-time spraying (for example, 0.1 seconds, etc.) is continuously performed at a second time interval shorter than the first intermittent spraying (for example, 1.0 second intervals), and third intermittent spraying where short-time spraying (for example, 0.1 seconds, etc.) is continuously performed at a third time interval even shorter than the second intermittent spraying (for example, 0.5 second intervals). Note that the instantaneous short-time spraying can be spraying of 1 second or less, preferably 0.5 second or less, more preferably 0.3 second or less, even more preferably 0.2 second or less, and most preferably 0.1 second or less. Also, the lower limit of the instantaneous short-time spraying can be 0.001 seconds or more, preferably 0.01 seconds or more, and preferably 0.05 seconds or more.

[0036] Further, when the manual spray button 16 is pressed (or when it is time to spray in the first intermittent spray, the second intermittent spray, or the third intermittent spray) when the spray setting is manual spray, the control unit 19d turns on the solenoid 17 for a predetermined instantaneous time (e.g., 0.1 second, etc.) at the moment of spraying to release the pipe 20a and allow the gas to pass through, and then turns off the solenoid 17 to close the pipe 20a and prevent the gas from passing through, thereby executing spray control. By performing spray control in this way, a negative pressure is generated in the spray liquid chamber 13 at the moment the solenoid 17 turns off, and the spray liquid in the liquid storage section 15a is supplied to the spray liquid chamber 13. Then, with the instantaneous supply of gas when the solenoid 17 is turned on next time, the spray liquid is sprayed from the spray port 12a together with the gas.

[0037] Further, the control unit 19d also executes an operation of displaying the current spray setting and error codes in the event of an error on the display unit 19a. Further, the control unit 19d executes these operations after being turned on by the operation button 19c, and stops and does not execute each operation when it is turned off by the operation button 19c. When the solenoid 17 is in the OFF state, the gas supplied from the pipe 20a flows through an exhaust path (not shown).

[0038] These manual spray button 16, solenoid 17, display unit 19a, switching button 19b, operation button 19c, and control unit 19d are supplied with power from the main body 30 through the electric wire 20b.

[0039] A spray amount adjustment unit 18 is provided downstream of the solenoid 17 in the handpiece 10. This spray amount adjustment unit 18 acts on the pipe 20a to adjust the gas supply amount when the solenoid 17 is turned on. Thereby, the amount of gas and spray liquid ejected in one spray can be adjusted. Note that the spray amount adjustment unit 18 may be configured to adjust the spray amount by changing the opening size of the spray port 12a. In this case, the spray range and spray intensity can also be adjusted.

[0040] The main body 30 is provided with a control unit 31, a power supply unit 32, and a gas supply unit 33. The control unit 31 controls the supply of gas by the gas supply unit 33. Specifically, when the operation start button 30a (see FIG. 1) is pressed and the power is turned on, the gas supply unit 33 is driven to start the supply of gas, and at the same time, the power supply from the electric wire 20b to the unit inside the handpiece 10 is started. When the operation start button 30a (see FIG. 1) is pressed again and the power is turned off, the supply of the gas and the power supply are stopped.

[0041] The power supply unit 32 supplies power to each unit. During the time when the power is on, the gas supply unit 33 drives the internal pump to continuously send gas through the pipe 20a to the spray gas chamber 14b of the handpiece 10. In this embodiment, the surrounding air is continuously sent as the gas, but a gas cylinder (not shown) can be attached to supply oxygen, or supply hydrogen, or supply an appropriate mixed gas, etc., and an appropriate gas can be supplied.

[0042] FIG. 3 is a flowchart showing the operation of the multi-component skin penetration system 1. When the operation start button 30a (see FIG. 1) is pressed and the power is turned on, the control unit 19d accepts the input of the first spray liquid from the liquid input hole 15b (step S1). The received first spray liquid is supplied from the liquid storage unit 15a to the spray liquid chamber 13.

[0043] The control unit 19d accepts the switching of the spray setting by the switching button 19b (step S2).

[0044] When the operation button 19c is pressed, the control unit 19d performs a first spray liquid spraying step of executing spraying according to the spray setting (step S3). That is, if the spray setting is manual spraying, spraying is executed at the timing when the manual spray button 16 is pressed. If the spray setting is the first intermittent spray, intermittent spraying at the first time interval is automatically and continuously executed. If the spray setting is the second intermittent spray, intermittent spraying at the second time interval is automatically and continuously executed. If the spray setting is the third intermittent spray, intermittent spraying at the third time interval is automatically and continuously executed.

[0045] When the spray setting is changed (step S4: YES), the control unit 19d accepts the change in the spray setting (step S2) and executes spraying with the changed spray setting (step S3).

[0046] When spraying this liquid for spraying (such as the first liquid for spraying, the second liquid for spraying, etc.), the tip of the spray cover 11 is brought into contact with the skin and spraying is performed. When the circular ring-shaped tip of the spray cover 11 is in full contact with the skin and spraying is performed, excess gas is discharged from the gas vent space 11a at the tip together with the spraying liquid. For this reason, most of the spraying liquid in the form of fine mist penetrates into the skin by the force of the discharge, and it is possible to prevent the excess gas from being discharged from the gas vent space 11a and pressing the skin too hard with the gas pressure.

[0047] That is, if spraying is performed in a state where the entire circumference of the tip of the spray cover 11 is in contact with the skin without the gas vent space 11a, the skin is pressed by the discharged gas and the spraying liquid, and since the gas in the spray cover 11 has increased, the skin remains in a state of being pressed and dented. Then, excessive pressure is applied to the skin, which may conversely stagnate blood flow and prevent the penetration of the spraying liquid. On the other hand, by discharging the excess gas from the gas vent space 11a, the skin is not continuously pressured, and the skin returns to its original normal state immediately after the gas and the spraying liquid are sprayed onto the skin. For this reason, the penetrated spraying liquid can dissolve into the blood flow and lymph flow without stress, and a light and appropriate stimulus is given to the skin to promote the blood flow and lymph flow, and the penetrated spraying liquid penetrates deeper.

[0048] If the spraying of the first liquid for spraying is not completed even after completion (step S5: NO), a second liquid for spraying spraying step for accepting the second liquid for spraying is performed (step S6). The accepted second liquid for spraying is supplied from the liquid storage unit 15a to the spray liquid chamber 13. After that, the processing from step S2 and subsequent steps is repeated, and spraying of the second liquid for spraying is executed.

[0049] With the above configuration and operation, the active ingredient can be atomized into fine mist and sprayed. Since the amount of the liquid for spraying used in one spray (instantaneous spray such as for 0.1 second) is small, even for a precious and expensive active ingredient, a large number of spraying times with a small amount sprayed each time can be obtained. Thus, it is possible to spray the entire face by spraying one place at a time in order, and it is also possible to spray the neck and décolletage as well. Further, it is possible to spray on the head, such as spraying the entire head or spraying a plurality of times on the part where intensive penetration is desired on the head.

[0050] When a growth factor agent such as a human stem cell culture solution is used for the penetrant (the first penetrant or the second penetrant), if it is sprayed on the skin of the face, neck, décolletage, etc., the firmness can be enhanced, and effects such as a lifting effect, an effect of eyes opening, an effect of reducing wrinkles, an effect of making the contour thinner, and an effect of making the skin fresh and rejuvenated can be obtained. In particular, when a human umbilical cord stem cell culture supernatant or a growth factor agent derived from a human umbilical cord stem cell culture supernatant is used, these effects can be obtained more remarkably.

[0051] Also, when spraying on a damaged or burned part on the skin using a human stem cell culture solution, an effect that the wound or burn heals earlier than usual can be obtained, and an effect that a beautiful state without a trace remains even after healing can be obtained. In particular, when a human umbilical cord stem cell culture supernatant or a growth factor agent derived from a human umbilical cord stem cell culture supernatant is used, these effects can be obtained more remarkably.

[0052] Also, when a growth factor agent such as a human stem cell culture solution is used for the penetrant (the first penetrant or the second penetrant) and it is sprayed on the head, hair growth effects such as a hair growth effect, an effect of increasing the volume of hair, an effect of the hairline advancing, an effect of enhancing the firmness of hair, an effect of strengthening the texture of hair, an effect of making hair thicker, and an effect of turning white hair back to black hair, and a young-looking effect can be obtained. In particular, when a human umbilical cord stem cell culture supernatant or a growth factor agent derived from a human umbilical cord stem cell culture supernatant is used, these effects can be obtained more remarkably.

[0053] In addition, when a sirtuin gene activator such as NMN is used in the penetrant (the first penetrant or the second penetrant), spraying it on the skin of the face, neck, décolletage, etc. can enhance firmness, and achieve effects such as a lifting effect, an effect of making the eyes look more open, an effect of reducing wrinkles, an effect of making the contour thinner, and an effect of making the skin look fresh and rejuvenated.

[0054] In addition, when spraying a sirtuin gene activator such as NMN on the damaged or burned areas of the skin, the effect of faster healing of the wounds or burns than normal can be obtained, and an effect of achieving a beautiful state without leaving a trace after healing can also be obtained.

[0055] Also, when a sirtuin gene activator such as NMN is used in the penetrant (the first penetrant or the second penetrant) and sprayed on the head, effects such as hair growth, an increase in hair volume, a forward movement of the hairline, an increase in hair firmness, an increase in hair texture, an increase in hair thickness, and a return of gray hair to black hair can be obtained.

[0056] And when a growth factor agent such as a human stem cell culture solution is used in one of the first penetrant and the second penetrant, and a sirtuin gene activator such as NMN is used in the other, for each of the above-mentioned effects, they can be obtained more significantly than in either one of the cases, and more effects can be obtained by combining both than by applying one of them in double the amount.

[0057] In particular, when the spraying of the first spraying solution using the first penetrant and the spraying of the second spraying solution using the second penetrant are carried out separately in sequence, these active ingredients can penetrate the skin one by one, and because each penetrates in sequence by blood flow and lymph flow, a synergistic effect can be exerted. In particular, when such a treatment is performed on the face, significant effects can be obtained on the change in contour and the change in eye size, and effects that may make people suspect that cosmetic surgery has been done can be obtained.

[0058] In addition, since the first spraying liquid and the second spraying liquid are sprayed by the spraying device 5, each spraying can be performed quantitatively and with a constant intensity. Therefore, regardless of the operator's arm movement, if the spraying is performed on the same location in the same order, the same effect can be obtained regardless of who performs the treatment.

Example

[0059] Examples of the multi-component skin penetration method using the multi-component skin penetration system 1 described above will be described below. The first penetrant 2, the second penetrant 3, and the conditioner 4 used in this example are as follows.

[0060] First penetrant 2: NMN <Details> · β-Nicotinamide mononucleotide · Particle size: 16 mesh or less · Bulk density: 0.6 g / ml or more · Purity 99% or more (composition ratio: 100%) · Processing: Micro granulation processing · Shape: White crystalline powder · Amount: 500 mg · Amount used in this example: 1 / 3 (= 166.67 mg)

[0061] Second penetrant 3: Human umbilical cord stem cell culture supernatant <Details> · Human mesenchymal stem cells derived from umbilical cord matrix (hMSC-UC) · Proliferating cell culture · Passage 2 (third culture) · Storage method: Frozen storage · Amount: 1 ml · Amount of material in this example: Total amount

[0062] Conditioner 4: Purified water · Purity 400,000 times that of tap water · Amount: 400 ml · Amount used in this example: (1) 3 ml is used for mixing with 166.67 g of NMN powder as the first penetrant 2... After mixing, about 3.17 ml of the second spraying liquid (2) Use 2 ml for mixing with 1 ml of the human umbilical cord stem cell culture supernatant as the second penetrant... After mixing, 3 ml of the first spray liquid

[0063] 0. Preparation First, perform prior application cleansing on the skin of the subject, and as a prior preparation, perform a massage to drain the lymph in the neck, décolletage, and face.

[0064] 1. Spraying of the first spray liquid (NMN) Using the spraying device 5, the first spray liquid was sprayed on the décolletage, neck, and face. The spraying was performed one by one in order with the tip of the spray cover 11 in contact with the skin. The total number of sprays was approximately 1,000 times.

[0065] 2. Spraying of the second spray liquid (human umbilical cord stem cell culture supernatant) Using the spraying device 5, the second spray liquid was sprayed on the décolletage, neck, and face. The spraying was performed one by one in order with the tip of the spray cover 11 in contact with the skin. The total number of sprays was approximately 1,000 times.

[0066] As a result of performing the above treatments on two subjects, the following effects were obtained.

[0067]

Table 1

[0068] In particular, since the change in the contour is easy to see, an example of the change in Subject 1 will be described with reference to FIG. 4. FIG. 4(A) is a photograph at the stage of completing the above "0. Preparation", that is, at the stage of finishing the lymph-draining massage. FIG. 4(B) is a photograph of the state after spraying NMN and then spraying the human umbilical cord stem cell culture supernatant. FIG. 4(C) is a photograph showing the state where the face contour in FIG. 4(A) before spraying and the face contour in FIG. 4(B) after spraying are superimposed. Thus, it is clearly understood that a great effect can be obtained by simply performing this treatment once, in which NMN and human stem cell culture solution are sequentially applied after draining the lymph (Before) (After).

[0069] In addition, the subject was said by a friend to have had plastic surgery the next day, and was also said by people met one week after the treatment to have had plastic surgery. That is, it can be seen that a great effect can be obtained by simply performing this treatment once, in which NMN and human stem cell culture solution are sequentially applied, and the effect is sustained.

Example

[0070] In Example 2, only the order of spraying was changed from Example 1. That is, the first penetrant 2 and the second penetrant 3 were interchanged as follows. (1) 2 ml is used for mixing with 1 ml of human umbilical cord stem cell culture supernatant as the first penetrant 2... After mixing, 3 ml of the first spraying liquid (2) 3 ml is used for mixing with 166.67 g of NMN powder as the second penetrant 3... After mixing, approximately 3.17 ml of the second spraying liquid Other than this, the content was the same as in Example 1, and massage, spraying of the first spraying liquid, and spraying of the second spraying liquid were performed in this order. As a result of performing the above treatment on two subjects, the following effects were obtained.

[0071]

Table 2

[0072] In addition, since subject 3 had atopy, the first spraying liquid (human umbilical cord stem cell culture supernatant) and the second spraying liquid (NMN) were also sprayed on the atopic part. As a result, improvement of atopic symptoms was observed, and improvement in skin moisture, firmness, and gloss of the atopic part was observed.

Example

[0073] Next, using the same formulation and procedure as in Example 1, spraying was performed on the scalp, which is a part of the skin, to confirm the hair growth and hair follicle activation effect. At this time, for the preliminary massage, the back of the neck and the scalp were massaged. The spraying solution and the procedure other than that were the same as in Example 1. A series of procedures was regarded as one time, and a total of four procedures were performed to measure the effect. The interval between each time was in the range of one week to one month. As a result of performing the procedure on two subjects, a clear hair growth effect (scalp skin beautifying effect) was observed in both subjects.

Example

[0074] Next, using the same formulation and procedure as in Example 2, spraying was performed on the scalp to confirm the hair growth and hair follicle activation effect. At this time, for the preliminary massage, the back of the neck and the scalp were massaged. The spraying solution and the procedure other than that were the same as in Example 2. A series of procedures was regarded as one time, and a total of four procedures were performed to measure the effect. The interval between each time was in the range of one week to one month. As a result of performing the procedure on two subjects, a clear hair growth effect (scalp skin beautifying effect) was observed in both subjects.

[0075] <Examples 5 to 10> Furthermore, the procedure was performed in various forms to measure the effect. Regarding the content of the preliminary massage, the spraying of the first penetrant 2, and the spraying of the second penetrant 3, the same formulation and method as in Examples 3 and 4 described above were used. Furthermore, an example in which holes were made on the surface of the skin with a roller (refer to the penetration promoter 105 described later) before spraying with the spraying device 5, and an example in which a shampoo containing proteoglycan was used for the usual shampoo separately from the procedure were added. In each example, a series of procedures was regarded as one time, and a total of four procedures were performed, and the interval between each time was in the range of one week to one month for implementation and measurement. As a result of the implementation, the results shown in Table 3 below were obtained.

[0076]

Table 3

[0077] This invention is not limited to the above-described embodiments and can be various other embodiments.

[0078] For example, before spraying the first spraying liquid, holes may be made on the surface of the skin with a roller (refer to the penetration promoter 105 described later) in which a plurality of fine needles of a predetermined length are arranged on the outer circumference of a cylinder, and then the spraying of the first spraying liquid and the spraying of the second spraying liquid may be continued. In this case, the penetration of the spraying liquid into the skin can be improved, and more effects can be obtained. In this case, the needles are preferably made of titanium. Also, the predetermined length can be 0.1 mm to 1 mm, preferably 0.1 to 0.5 mm, and preferably 0.25 mm.

[0079] Also, as a preliminary preparation, massaging to promote lymph flow improves blood flow and lymph flow, so better effects (effects by massage + effects by spraying) can be obtained, but the spraying of the first spraying liquid and the spraying of the second spraying liquid may be performed without such preliminary preparation. Even in this case, clear changes can be obtained before and after spraying.

[0080] Also, the first liquid for spraying was sprayed 1,000 times, and the second liquid for spraying was sprayed 1,000 times. However, it is not limited to this, and it can be 500 times each, or 300 times each, or conversely, increased to 1,500 times each, etc., and can be an appropriate number of times. The number of sprays depends on the area of the skin to be sprayed, but for one type of liquid for spraying, it can be 10 times or more, preferably 50 times or more, more preferably 100 times or more, even more preferably 300 times or more, and suitably 500 times or more. Also, the upper limit of the number of sprays for one type of liquid for spraying can be 3,000 times or less, preferably 2,000 times or less, more preferably 1,500 times or less, and suitably 1,000 times or less.

[0081] With respect to the area of the skin to be coated, the area covered inside the tip of the circular spray cover 11 can be coated at 40% or more of the coating area, preferably 50% or more, more preferably 60% or more, even more preferably 70% or more, still more preferably 80% or more, and suitably 90% or more. Also, spraying at the same position can be done once, but spraying two or more times may allow more active ingredients to penetrate. In particular, it is preferable to spray two or more times partially on parts such as fine wrinkles around the eyes, nasolabial folds, and forehead wrinkles that need to be intensively cared for. Thereby, the penetration of the active ingredient can be increased in the parts that need to be intensively cared for, and a more preferable effect can be obtained in the parts that need to be intensively cared for.

[0082] Also, for the liquid for spraying using the first penetrant 2 and the second penetrant 3, either the simultaneous input of all components in which both are mixed with the conditioner 4 and then put into the liquid storage part 15a, or the sequential input of components in which the first penetrant 2 and the conditioner 4 are mixed to form the first liquid for spraying and put into the liquid storage part 15a for spraying, and then within a predetermined time, the second penetrant 3 and the conditioner 4 are mixed to form the second liquid for spraying and put into the liquid storage part 15a can be used.

[0083] The predetermined time from the completion of spraying the liquid for the first spraying to the start of spraying the liquid for the second spraying in the case of sequential input of components can be within one week, preferably within 48 hours, preferably within 24 hours, more preferably within 12 hours, even more preferably within 6 hours, even more preferably within 3 hours, even more preferably within 1 hour, even more preferably within 30 minutes, and suitably within 10 minutes. By sequentially inputting in this way, the components of the first penetrant 2 can be sufficiently penetrated, and the components of the second penetrant 3 can also be sufficiently penetrated. Then, the effects of both components can be obtained simultaneously, and a synergistic effect can be exerted. In particular, by shortening the predetermined time from the spraying of the liquid for the first spraying (the first penetrant 2 + the conditioner 4) to the spraying of the liquid for the second spraying (the second penetrant 3 + the conditioner 4), the synergistic effect by both components can be enhanced. Also, by sequentially inputting rather than mixing all and inputting simultaneously, each component can be firmly penetrated.

[0084] When inputting all components at once, the overall spraying time can be shortened compared to sequential input by component. For example, in the case of sequential input by component, after spraying the liquid for the first spraying 1,000 times on each part of the face (the neck and décolletage may be added further), it is necessary to spray the liquid for the second spraying 1,000 times again on each part of the face (the neck and décolletage may be added further), and it takes time for a total of 2,000 sprays. However, in the case of inputting all components at once, since the spraying on each part of the face (the neck and décolletage may be added further) can be completed in 1,000 times in total, it can be shortened to half the time.

[0085] In the case of simultaneous input of all components, in addition to the first penetrant 2 and the second penetrant 3, another third penetrant (an agent composed of a third active ingredient) can be further mixed and then all components can be simultaneously input and sprayed. The third penetrant can be an appropriate substance. For example, it can be a cannabinoid (third active ingredient) such as CBD (cannabidiol), CBN (cannabinol), or CBG (cannabigerol). When it is such a cannabinoid, it is preferably one from which THC (tetrahydrocannabinol) has been removed.

[0086] In addition, the third penetrant can also be a proteoglycan. In this case, especially when applied to the head, the effects on the hair (such as the hair growth effect, the effect of increasing hair volume, the effect of the hairline advancing, the effect of enhancing hair stiffness, the effect of strengthening hair texture, the effect of thickening hair, the effect of turning gray hair back to black hair, etc.) can be improved.

[0087] In the case of sequential input by component, after spraying the first liquid for spraying (the first penetrant 2 + conditioner 4) and the second liquid for spraying (the second penetrant 3 + conditioner 4), the third liquid for spraying (the third penetrant + conditioner 4) can also be sprayed within a predetermined time. Furthermore, it is also possible to add, for example, spraying the third liquid for spraying (the fourth penetrant + conditioner 4) within the predetermined time.

[0088] Also, the first active ingredient was applied (sprayed) in this order with NMN (sirtuin gene activator) and the second active ingredient with human stem cell culture solution (growth factor agent), but the order can be reversed so that the first active ingredient is the human stem cell culture solution (growth factor agent) and the second active ingredient is NMN (sirtuin gene activator) and applied (sprayed) in this order. In this case as well, each active ingredient can be sequentially penetrated to exert their respective synergistic effects.

[0089] Also, either the first active ingredient or the second active ingredient can be the human stem cell culture solution and the other can be a proteoglycan, and they can be applied (sprayed) in order respectively. In this case as well, the respective effects can be fully exerted to obtain a synergistic effect. Also in this case, by applying to the scalp, a high effect of hair growth and hair nourishment can be obtained.

[0090] In addition, the method of application is preferably spraying as described above, but is not limited thereto, and other methods such as applying to the skin by hand or permeating a pack or the like and placing it on the skin can also be used. At that time, by making a plurality of small holes in the skin using a fine needle and then performing the application, the permeability of the active ingredient can be enhanced. Also in this case, a certain degree of effect can be obtained, and the synergistic effect of each active ingredient can be exerted by applying them in order.

[0091] Further, this first embodiment can be provided as a composition kit (beauty kit, skin external use kit). This composition kit can be a set of the first penetrant 2 and the second penetrant 3, a set of the first penetrant 2, the second penetrant 3 and the conditioner 4, a set of the first penetrant and the conditioner 4, a set of the second penetrant and the conditioner 4, or a set of the first penetrant 2, the second penetrant 3, the conditioner 4 and the spraying device 5 (penetration promoter). In this case, the user can purchase these, adjust the first penetrant 2 with the conditioner 4 and apply it to the skin with the spraying device 5, and then adjust the second penetrant 3 with the conditioner 4 and apply it to the skin with the spraying device 5 and use it in such a form.

[0092] Similarly, the above-described Examples 4 to 10 can be provided as a composition kit for hair growth, that is, a hair growth kit. This hair growth kit can be a set of the first penetrant 2 and the second penetrant 3, a set of the first penetrant 2, the second penetrant 3 and the conditioner 4, a set of the first penetrant and the conditioner 4, a set of the second penetrant and the conditioner 4, or a set of the first penetrant 2, the second penetrant 3, the conditioner 4 and the spraying device 5 (penetration promoter). In this case, the user can purchase these, adjust the first penetrant 2 with the conditioner 4 and apply it to the skin with the spraying device 5, and then adjust the second penetrant 3 with the conditioner 4 and apply it to the skin with the spraying device 5 and use it in such a form.

[0093] FIG. 5 is a configuration diagram showing another example of the composition kit. The composition kit 101 includes a first skin care product 120 with a first penetrant 102 contained in a first container 125, a second skin care product 130 with a second penetrant 103 contained in a second container 135, a liquid container product 140 with a liquid 104 serving as an adjuster contained in a liquid storage container 145, and a penetration promoter 105 that promotes the penetration of beauty ingredients into the skin.

[0094] The first penetrant 102 can be the same as the above-described first penetrant 2 or can be the above-described first penetrant 2 with other components added thereto. The second penetrant 103 can be the same as the above-described second penetrant 3 or can be the above-described second penetrant 3 with other components added thereto. Other components to be included can be appropriately selected components such as niacinamide, collagen, hyaluronic acid, elastin, retinol (vitamin A), vitamin C, ceramide, amino acids, neelwan, fullerene, honey extract, or a plurality of these.

[0095] Niacinamide, also known as nicotinamide, D-melanin, or wrinkle niacin, is a type of vitamin B. Niacinamide can be contained in an amount of 0.01% or more, preferably 0.05% or more, more preferably 0.1% or more, even more preferably 0.5% or more, and still more preferably 1% or more. Also, niacinamide can be 70% or less, preferably 50% or less, more preferably 30% or less, even more preferably 20% or less, still more preferably 10% or less, and most preferably 5% or less, and preferably 3.5% or less.

[0096] The first penetrant 102 and the second penetrant 103 are preferably powders that dissolve in a liquid such as water, purified water, or saline solution. This allows the first penetrant 102 and the second penetrant 103 to be stored in a stable state and used as a liquid during use.

[0097] It is preferable that the first skin care product 120 and the second skin care product 130 sterilize the first container 125 and the second container 135, respectively accommodate the powdery first penetration enhancer 102 and the powdery second penetration enhancer 103 in a clean room, and close and seal the lids 123 and 133, respectively. Thereby, it becomes possible to provide the first skin care product 120 and the second skin care product 130 without containing a preservative component.

[0098] The penetration promoter 105 is a needle roller type beauty instrument in which a cylindrical roller 152 is rotatably supported at one end of a rod-shaped handle 153 serving as a handle, and a large number of needles 151 protrude from the surface of the roller 152. More than half of the base side of the needle 151 is embedded in the roller 152 and the tip protrudes from the surface of the roller 152. The protruding length of the needle 151 can have a lower limit of 0.1 mm or more, preferably 0.2 mm or more, can have an upper limit of 1.0 mm or less, preferably 0.7 mm or less, more preferably 0.5 mm or less, even more preferably 0.4 mm or less, and even more preferably 0.3 mm or less. When used for home use, it is preferably 0.25 mm within this range. The material of the needle 151 can be a metal material, preferably titanium. By making the needle 151 titanium, the antibacterial property can be enhanced. Further, the needles 151 are arranged at equal intervals in a plurality of places on the circumferential outer periphery of the roller 152. The number of the needles 151 can have a lower limit of 200 or more, preferably 300 or more, and more preferably 400 or more. Also, the number of the needles 151 can have an upper limit of 1,000 or less, preferably 900 or less, and more preferably 800 or less. In this embodiment, 540 needles 151 are arranged in 6 rows in parallel and at equal intervals, with one row of 60 needles 151 arranged in a circle at equal intervals. In this embodiment, the penetration promoter 105 is a needle roller type beauty instrument, but the present invention is not limited to this, and the above-described spray device 5 (see FIG. 1) may be used as the penetration promoter 105.

[0099] Both the first container 125 and the second container 135 are composed of main bodies 124, 134 made of glass bottles and lids 123, 133 that seal the openings above the main bodies 124, 134. The first penetrant 102 or the second penetrant 103 is respectively accommodated in the internal spaces of the main bodies 124, 134.

[0100] The sizes of the main bodies 124, 134 are of a sufficient volume to dissolve the first penetrant 102 or the second penetrant 103, which is a powder, in the liquid 104. This volume can have a lower limit of 1 ml or more, preferably 2 ml or more, more preferably 3 ml or more, and even more preferably 4 ml or more. Also, this volume preferably has an upper limit of 20 ml or less, more preferably 15 ml or less, still more preferably 10 ml or less, and even more preferably 7 ml or less. In this embodiment, the volume is set to 4 ml, and a configuration is adopted in which 3 ml of the liquid 104 is introduced to dissolve the first penetrant 102 or the second penetrant 103.

[0101] Next, Examples 11 to 18 in which the user used the composition kit 101 for home use will be described together with Table 4. In Examples 11 to 18, the first penetrant 102 and the second penetrant 103 were each dissolved in 3 ml of purified water (liquid 104) for use.

[0102] Also, in Examples 11 to 14, a needle roller type beauty device was used as the penetration promoter 105, and after making holes in the facial skin with the needle roller type beauty device, the first penetrant was applied and the second penetrant was applied. The effects after performing this series of operations 5 times were measured, and for the 5 measurements, the interval between each measurement was in the range of 1 week to 1 month.

[0103] Also, in Examples 15 to 18, a spray device 5 was used as the penetration promoter 105, and after applying (spraying) the first penetrant with this spray device 5, the second penetrant was applied (sprayed) with the spray device 5. In Examples 15 to 18, the effects in one implementation were measured.

[0104] In addition, since Examples 11 to 18 are for home use, no prior massage or the like was performed. Also, for the first penetrant and the second penetrant in Examples 11 to 18, either 1 mg of human umbilical cord stem cell culture supernatant (freeze-dried powder) is dissolved in 3 ml of purified water (Liquid 104) for use, or 150 mg of NMN (powder) is dissolved in 3 ml of purified water (Liquid 104) for use, or 1 mg of proteoglycan (freeze-dried powder) is dissolved in 3 ml of purified water (Liquid 104) for use.

[0105]

Table 4

[0106] As shown in Table 4, the effects were demonstrated in all cases. Figure 6 is an explanatory diagram illustrating the before and after of Example 16 with photographs. In this example, a 76-year-old woman is the subject.

[0107] Figure 6(A) is a photograph of the state before the start, Figure 6(B) is a photograph of the state after spraying NMN (powder) dissolved in purified water with the spraying device 5, and Figure 6(C) is a photograph of the state after spraying the human umbilical cord stem cell culture supernatant (freeze-dried powder) dissolved in purified water with the spraying device 5. In this example, the spraying of NMN (powder) was carried out over about 15 minutes, and after an interval of about 10 minutes for photography and the next preparation, the spraying of the human umbilical cord stem cell culture supernatant (freeze-dried powder) was carried out over about 15 minutes, and the whole process from start to finish took about 40 minutes.

[0108] The lifting effect, eye opening, wrinkle improvement, cheek position, skin firmness, skin gloss, and whitening all show remarkable effects. Figure 6 compares the skin from the upper lip to the nose, which is the most obvious. It is clearly visible that from before the treatment in Figure 6(A) to after NMN in Figure 6(B), the wrinkles become fewer and shallower. Also, compared with the improved state in Figure 6(B) after spraying NMN (powder) with the spraying device 5, it can be visually confirmed that the state in Figure 6(C) after spraying the human umbilical cord stem cell culture supernatant (freeze-dried powder) with the spraying device 5 is further improved. Thus, by applying the first penetrant 102 and the second penetrant 103 in sequence, a two-stage improvement is obtained, resulting in a high anti-aging effect.

[0109] Next, Examples 19 to 57 will be described together with Tables 5 and 6. These examples were carried out with various combinations of the sirtuin gene activator and the growth factor agent, and the effects were measured.

[0110]

Table 5

[0111]

Table 6

[0112] The sirtuin gene activator uses one or more of NAD+, NMN, 5-deazafavin, and niacinamide, and all are used by dissolving the powdery substances in purified water as the solvent.

[0113] The growth factor agent uses one or more of EGE, EGF, PDGF, exosomes, and human stem cell culture supernatant, and all of them are in powder form and dissolved in purified water as the solvent for use. As FGF, FGF1 is used. The human stem cell culture supernatant uses a human umbilical cord stem cell culture supernatant (freeze-dried powder) derived from the umbilical cord. Also, EGE, EGF, PDGF, exosomes, and human stem cell culture supernatant used as the growth factor agent are all in powder form by the freeze-drying method and dissolved in purified water as the solvent for use.

[0114] In Table 5 and Table 6, the numerical values in each example indicate volume, and the symbol "-" indicates that the component is not formulated. Specifically, the numerical values (0.2, 0.5, 1, 2, 5) of the growth factor agent all indicate volume and the unit is ml. The numerical values (50, 100, 150, 200, 250, 300, 500) of the sirtuin gene activator all indicate volume and the unit is mg. The numerical values (2, 3) of the solvent (purified water) all indicate volume and the unit is ml.

[0115] For Examples 19 to 57, no prior massage or the like was performed. The first penetrant dissolved in the solvent was sprayed onto the entire facial skin and the skin under the chin using the spray device 5 also used in Example 1. After a pause of about 3 minutes, the second penetrant dissolved in the solvent was sprayed onto the entire facial skin and the skin under the chin using the spray device 5.

[0116] As described in the effects of each example, improvements were observed in all of the lift-up effect, eye opening, wrinkle improvement, cheek position, skin firmness, skin gloss, and whitening effects. As the sirtuin gene activator, the example using NAD+ obtained the highest effect.

[0117] In addition, for Examples 49 to 52 where NAD+ was used as a sirtuin gene activator and dissolved in purified water and sprayed onto the skin with a spraying device 5 without using a growth factor agent, although the effect was lower compared to the examples using a growth factor agent, a certain degree of effect was obtained. Regarding Examples 49 to 52, NAD+ and a growth factor agent may be mixed, dissolved in purified water, and sprayed with the spraying device 5. In this case, as the growth factor agent, a growth factor, exosome, stem cell culture supernatant, or a plurality of these can be used, and it is preferably an exosome. Even in this case, although the effect is lower than when the growth factor agent and the sirtuin gene activator are separately and sequentially applied, a certain degree of effect is obtained. Specifically, when 1 mg of exosome and 200 mg of NAD+ were mixed and dissolved in 3 ml of purified water and sprayed with the spraying device 5, significant improvements were observed in eye opening, wrinkle improvement, firmness of the skin, gloss of the skin, and whitening, and improvements were also observed in the lifting effect and the position of the cheeks.

Industrial Applicability

[0118] This invention can be used in industries such as beauty salons and hair growth salons.

Explanation of Symbols

[0119] 1… Multi-component skin penetration system 12a… Spray opening 15a… Liquid storage part 17… Solenoid 19d… Control part 33… Gas supply part

Claims

1. applying a first composition containing one of a sirtuin gene activator and a growth factor agent; applying a second composition containing the other of the sirtuin gene activator and the growth factor agent; The sirtuin gene activator is any one or more selected from NAD+, NMN, 5-deazaflavin, and niacinamide; The growth factor agent is any one or more selected from EGF, FGF, PDGF, exosomes, and human stem cell culture agents. Skin beauty treatments (excluding medical procedures).

2. The sirtuin gene activator is any one or more selected from NAD+, NMN, and 5-deazaflavin. The skin cosmetic method according to claim 1 (excluding medical procedures).

3. The growth factor agent is any one or more selected from EGF, FGF, exosomes, and human umbilical cord stem cell culture supernatant. The skin cosmetic method according to claim 1 (excluding medical procedures).

4. A composition kit for use in the skin beauty method according to claim 1, 2 or 3, comprising: a first skin care product in which a first composition containing one of the sirtuin gene activator and the growth factor agent is contained in a first container; and a second skin application device containing a second composition containing the other of the sirtuin gene activator and the growth factor agent in a second container. Composition kit.

5. the first composition is in powder form; The second composition is also in powder form; A liquid container that contains a liquid that dissolves the first composition and the second composition in a third container. The composition kit according to claim 4.

6. A liquid storage section that stores a liquid to be sprayed containing an active ingredient; a spray nozzle for spraying the spray liquid contained in the liquid container; A gas supply unit that supplies a gas; a spray control unit that instantly supplies gas supplied from the gas supply unit to the spray nozzle to spray the spray liquid together with the gas from the spray nozzle, A multiple-component skin penetration method for penetrating multiple components into the skin by the skin beauty method according to claim 1, 2, or 3, A first penetrant containing the first composition for preparing the spray liquid and a second penetrant containing the second composition different from the first composition for preparing the spray liquid are adjusted with an adjuster, and the spray liquid is manually or intermittently sprayed at a discharge speed of 100 km / h or more. Multiple ingredient skin penetration method.

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