Moisturizing and antioxidant composition containing Campanumoea maculata extract and PDRN

Abstract

The invention discloses a moisturizing and antioxidant composition containing a Campanumoea maculata extract and PDNR, and belongs to the technical field of cosmetics. The composition is prepared from the following components: an extract of barks of the calyx ampelopsis, an extract of fruits of annona squamosa, an extract of leaf cells of desert rose, an extract of fruits of fructus corni and polydeoxyribonucleotide (PDRN), and the composition has good moisturizing and anti-oxidation effects through the synergistic effect of the five components of the extract of the barks of the calyx ampelopsis, the extract of the fruits of the fructus corni and the polydeoxyribonucleotide (PDRN).

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a moisturizing and antioxidant composition containing Euphorbia neriifolia extract and PDRN. BACKGROUND

[0002] Skin oxidative stress is a core pathological factor that accelerates aging, which leads to collagen degradation and skin barrier function decline through active oxygen (ROS)-mediated cell damage pathways. At present, natural plant extracts (such as polyphenols and terpenoids) are widely used in anti-aging cosmetics due to their antioxidant activity. However, single plant active ingredients have the following significant limitations in application: Limited target: single component is difficult to comprehensively cover the multi-pathway damage mechanism of oxidative stress, resulting in insufficient antioxidant efficacy; High effective dose: high concentration is often required to achieve the desired effect, which may cause skin irritation or formulation stability problems; Lack of synergistic mechanism: conventional compounding schemes mostly rely on simple combination, and there is a lack of synergistic effect among components, making it difficult to break through the efficacy bottleneck.

[0003] In addition, the synergistic application of plant extracts and bioactive ingredients (such as nucleic acid substances) in the prior art is not sufficient, especially lacking a solution that can simultaneously optimize antioxidant and moisturizing efficacy and is suitable for sensitive skin. Therefore, it is urgent to develop a compounding system that realizes multi-component synergistic effect through scientific proportioning, to break through the limitations of existing technologies with low dose and multi-target strategy. SUMMARY

[0004] The purpose of the present application is to provide a moisturizing and antioxidant composition containing Euphorbia neriifolia extract and PDRN, which uses natural plant extracts and PDRN for compounding, has high safety, and has significant moisturizing and antioxidant efficacy.

[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions: In a first aspect, the present application provides a moisturizing and antioxidant composition containing Euphorbia neriifolia extract, which comprises Euphorbia neriifolia bark extract, Syagrus romanzoffiana fruit extract, Adenium obesum leaf cell extract, Cornus officinalis fruit extract, and PDRN in a mass ratio of 1:0.8-1:2-3:1-2:1-1.5.

[0006] In a second aspect, the present application provides the use of the moisturizing and antioxidant composition containing Euphorbia neriifolia extract of the first aspect in the preparation of cosmetics with moisturizing and antioxidant efficacy.

[0007] Preferably, the dosage form of the cosmetic is any one of a cream and an essence.

[0008] In a third aspect, the present application provides a serum with moisturizing and antioxidant effects, which is composed of the following components in mass percentage: The moisturizing and antioxidant composition containing Euphorbia maculata extract in the first aspect: 4-6 wt%; Dipotassium glycyrrhizinate: 0.1-0.3 wt%; Hydroxypropyl methyl cellulose: 0.5-1.5 wt%; Deionized water is supplemented to 100 wt%.

[0009] In a fourth aspect, the present application provides a preparation method of the serum in the third aspect, which comprises the following steps: A1: uniformly mix Euphorbia maculata bark extract, Annas comosa fruit extract, Adenium obesum leaf cell extract, Cornus officinalis fruit extract, dipotassium glycyrrhizinate, and hydroxypropyl methyl cellulose using 2 / 3 of deionized water at 50-60℃, and cool to 2-8℃ to obtain a mixed solution A; A2: mix PDRN using the remaining deionized water at 2-8℃ to obtain a mixed solution B; A3: uniformly mix the mixed solution A and the mixed solution B at 2-8℃ to obtain the serum, seal the serum in an ampoule, and store it at 2-8℃ in the dark.

[0010] Compared with the prior art, the present application has the following beneficial effects: The present application synchronously breaks through the antioxidant and moisturizing effects through the synergistic matching of active ingredients and low-temperature process innovation, and takes into account the safety and universality, providing an efficient solution for sensitive skin care, as follows: 1. Synergistic antioxidant effect The present application compounds Euphorbia maculata bark extract, Annas comosa fruit extract, Adenium obesum leaf cell extract, Cornus officinalis fruit extract, and PDRN in a specific mass ratio to form a synergistic mechanism, significantly improving the antioxidant efficiency, and having no negative effect on skin cell activity.

[0011] 2. Long-acting moisturizing optimization The serum of the present application is prepared by low-temperature stepwise premixing and then mixing, so that the prepared serum has significantly enhanced skin absorption efficiency and achieves a long-lasting hydration and moisturizing effect.

[0012] 3. Safety and universality The serum of the present application is mild and non-irritating, suitable for people with sensitive skin, and has high biological safety and stability. DETAILED DESCRIPTION

[0013] For a better understanding of the present application, it is described further below in connection with specific examples, in which the terms used in the examples are intended to describe specific embodiments and do not constitute a limitation to the scope of protection of the present application.

[0014] The experimental methods in the following tests without specific conditions are generally according to the conventional conditions, or according to the conditions suggested by the manufacturers. Unless otherwise specified, the percentages and parts are calculated by weight.

[0015] Part of the raw materials and sources are as follows: PDRN: purchased from Regenesis (Shandong) Biotechnology Co., Ltd., model: RJMPDRN-850K.

[0016] Pachysandra terminalis bark: purchased from Shanghai Qianfei Chemical Co., Ltd.

[0017] Cherimoya fruit extract: purchased from Shanghai Yanganfu Industry Co., Ltd. Desert rose leaf cell extract: purchased from Shanghai Xinsengmeike Biological Co., Ltd. Fructus corni: purchased from Guangzhou Nuoyuan Biological Technology Co., Ltd. Other raw materials or reagents are all conventional market sales.

[0018] The preparation method of the Pachysandra terminalis bark extract described in the present application refers to the preparation method of Pachysandra terminalis bark extract 1 disclosed in the patent "Antioxidant composition containing Pachysandra terminalis bark extract and application thereof" (application number: CN202510334360.3), and the specific steps are as follows: S1. Dry the Pachysandra terminalis bark to a water content of ≤5%, and grind it into coarse powder; S2. Mix the Pachysandra terminalis bark coarse powder with rapeseed oil and polyglyceryl-3 diisostearate, heat to 55℃, and stir at 300 rpm for 20 min to obtain a mixed solution; S3. Under N2 atmosphere, ultrasonic-assisted extraction is performed on the mixed solution in S2 to obtain an extract, the extraction temperature is 55℃, the ultrasonic frequency is 30 kHz, and the extraction time is 45 min; S4. Filter the extract to remove insoluble substances, centrifuge, and take the supernatant to obtain the Pachysandra terminalis bark extract; In step S2, the mass ratio of Pachysandra terminalis bark coarse powder, rapeseed oil and polyglyceryl-3 diisostearate is 1:3:0.2.

[0019] The preparation method of the Fructus corni extract refers to the preparation method of the Fructus corni extract rich in morroniside disclosed in the patent "Fructus corni extract rich in morroniside and preparation method and application thereof" (application number: CN202510000746.0), and the specific steps are as follows: (1) Take the Fructus Corni Schizandrae medicinal material (produced in Qinling, Shaanxi), dry and crush into powder, and reserve. Take 1 Kg of 60% ethanol according to the ratio of 1:10, adjust the pH to 5, and then put it into the extraction tank; then add the Fructus Corni Schizandrae powder and mix evenly to obtain the mixture to be extracted.

[0020] (2) Add 0.5% pectinase and cellulase of the dry weight of the Fructus Corni Schizandrae to the mixture to be extracted, wherein the mass ratio of pectinase to cellulase is 1:1; first constant temperature 50℃ for 1h, then increase the temperature to 70℃ for 1h; then increase the temperature to 90℃ for 30min to obtain the crude extract; then reduce the temperature to 50℃, and pour the crude extract into the storage tank; centrifuge the crude extract, collect the filtrate and discard the residue; add the filtrate to the concentrator in three times, and then centrifuge to obtain the crude extract. Note that the circulation extraction refers to the operation of promoting the contact between the material and the solvent by stirring, shaking, etc.

[0021] (3) Dilute the crude extract with 10 times pure water, add a polyamide packed column for chromatography, and then elute with pure water, wherein the flow rate of the sample loading is controlled at 2 times the column volume / h, 6 times the column volume of pure water is used for elution at a flow rate of 2 times the column volume / h, and the eluate is collected from the start of the sample loading; the collected eluate is added to the concentrator for concentration for 3 times, and then centrifuged to obtain the Fructus Corni Schizandrae extract. The pH value of the Fructus Corni Schizandrae extract is 5.6, and the relative density is 1.084.

[0022] The mass ratio of each component in the composition with moisturizing and antioxidant effects according to the present application is as follows: Composition 1: The mass ratio of the bark extract of Malpighia emarginata DC, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Fructus Corni Schizandrae, and PDRN is 1:0.9:2.5:1.5:1.3.

[0023] Composition 2: The mass ratio of the bark extract of Malpighia emarginata DC, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Fructus Corni Schizandrae, and PDRN is 1:1:3:2:1.

[0024] Composition 3: The mass ratio of the bark extract of Malpighia emarginata DC, the fruit extract of Annona glabra, the leaf cell extract of Adenium obesum, the fruit extract of Fructus Corni Schizandrae, and PDRN is 1:0.8:2:1:1.5.

[0025] Composition ①: Different from composition 1, composition ① does not contain the extract of the bark of the tree of the genus Clockflower, and is composed of the extract of the fruit of the tree of the genus Chimon, the extract of the leaf cell of the desert rose, the extract of the fruit of the tree of the genus Cornus, and PDRN, with a mass ratio of 0.9:2.5:1.5:1.3; and the rest of the raw materials are the same as those in composition 1.

[0026] Composition ②: Different from composition 1, composition ② does not contain the extract of the fruit of the tree of the genus Chimon, and is composed of the extract of the bark of the tree of the genus Clockflower, the extract of the leaf cell of the desert rose, the extract of the fruit of the tree of the genus Cornus, and PDRN, with a mass ratio of 1:2.5:1.5:1.3; and the rest of the raw materials are the same as those in composition 1.

[0027] Composition ③: Different from composition 1, composition ③ does not contain the extract of the leaf cell of the desert rose, and is composed of the extract of the bark of the tree of the genus Clockflower, the extract of the fruit of the tree of the genus Chimon, the extract of the fruit of the tree of the genus Cornus, and PDRN, with a mass ratio of 1:0.9:1.5:1.3; and the rest of the raw materials are the same as those in composition 1.

[0028] Composition ④: Different from composition 1, composition ④ does not contain the extract of the fruit of the tree of the genus Cornus, and is composed of the extract of the bark of the tree of the genus Clockflower, the extract of the fruit of the tree of the genus Chimon, the extract of the leaf cell of the desert rose, and PDRN, with a mass ratio of 1:0.9:2.5:1.3; and the rest of the raw materials are the same as those in composition 1.

[0029] Composition ⑤: Different from composition 1, composition ⑤ does not contain PDRN, and is composed of the extract of the bark of the tree of the genus Clockflower, the extract of the fruit of the tree of the genus Chimon, the extract of the leaf cell of the desert rose, and the extract of the fruit of the tree of the genus Cornus, with a mass ratio of 1:0.9:2.5:1.5; and the rest of the raw materials are the same as those in composition 1.

[0030] Composition ⑥: Different from composition 1, composition ⑥ is composed of the extract of the bark of the tree of the genus Clockflower, the extract of the fruit of the tree of the genus Chimon, the extract of the leaf cell of the desert rose, the extract of the fruit of the tree of the genus Cornus, and PDRN, with a mass ratio of 1:0.5:1:3:2.

[0031] The mass percentage and preparation steps of each component in the serum with the moisturizing and antioxidant effects according to the application are as follows: Serum 1: composition 1: 6wt%, dipotassium glycyrrhizinate: 0.3wt%, hydroxypropyl methyl cellulose: 1.5wt%, and deionized water is supplemented to 100wt%. Preparation method: A1: Mix the extract of Euphorbia neriifolia, the extract of Annona montana, the extract of Adenium obesum, the extract of Cornus officinalis, dipotassium glycyrrhizinate, hydroxypropyl methyl cellulose with 2 / 3 of deionized water at 55°C, and cool to 8°C to obtain a mixed solution A; A2: Mix PDRN with the remaining deionized water at 8°C to obtain a mixed solution B. A3: Mix the mixed solution A and the mixed solution B at 8°C to obtain a serum, and seal the serum and store it at 8°C in the dark.

[0032] Serum 2: Composition 2: 5wt%, dipotassium glycyrrhizinate: 0.2wt%, hydroxypropyl methyl cellulose: 1wt%, and deionized water to 100wt%. The preparation steps are the same as those of the serum 1.

[0033] Serum 3: Composition 3: 4wt%, dipotassium glycyrrhizinate: 0.1wt%, hydroxypropyl methyl cellulose: 0.5wt%, and deionized water to 100wt%. The preparation steps are the same as those of the serum 1.

[0034] Serum ①: Composition 1: 6wt%, dipotassium glycyrrhizinate: 0.3wt%, hydroxypropyl methyl cellulose: 1.5wt%, and deionized water to 100wt%. Preparation method: Mix deionized water with each component in Composition 1, dipotassium glycyrrhizinate, and hydroxypropyl methyl cellulose at 55°C, and cool to 8°C to obtain a serum, seal it, and store it at 8°C in the dark.

[0035] Test 1: Antioxidant test determination 1-1: ABTS inhibition rate determination ABTS reacts with potassium persulfate to generate green ABTS·. + Free radicals. This radical has a maximum absorption at 752nm. Therefore, by detecting the absorbance at 752nm, the concentration can be determined. After a substance is added to the ABTS· + free radical working solution, if the absorbance at 752nm decreases, it indicates that the substance has free radical scavenging activity and is an antioxidant.

[0036] Test samples: Composition 1-3, Composition ①-⑥.

[0037] Test sample preparation: Prepare the test sample into a mixed solution of 0.1mg / mL using deionized water.

[0038] Use the total antioxidant capacity (ABTS method) test kit (purchased from Shanghai Zymen, model ml092653) to detect the sample solution, and the detection method strictly follows the kit instructions.

[0039] The experimental results are shown in Table 1.

[0040] Table 1 Effect of composition on ABST clearance rate Sample name Radical ABST clearance rate (%) Composition 1 48.33 Composition 2 46.93 Composition 3 46.81 Composition 1 36.57 Composition 2 33.76 Composition 3 27.42 Composition 4 36.81 Composition 5 36.59 Composition 6 41.31 As can be seen from the results of comparative compositions 1-3, the antioxidant effect of composition 1 is the best, and therefore composition 1 is selected as the best embodiment.

[0041] As can be seen from the results of comparative composition 1 and compositions ①-⑥, in the composition provided by the present application, there is a significant synergistic effect between the four components of Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract and PDRN. Specifically, as can be seen from the results of comparative composition 1 and compositions ①-⑤, there is a certain synergistic effect between the four components of Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract in the present application; as can be seen from the results of comparative composition 1 and composition ⑥, the composition obtained by compounding the Euclea natalensis bark extract, Annona glabra fruit extract, Adenium obesum leaf cell extract and Cornus officinalis fruit extract in the quality ratio defined in the present application with PDRN has a better ABST free radical clearance rate.

[0042] 1-2: Composition ROS assay for clearing human keratinocytes Sample solution: The sample solution is a mixture prepared by formulating compositions 1-3 and compositions ①-⑥ into a solution with a concentration of 5 mg / mL using Promocell complete culture medium.

[0043] Cell culture: Human primary skin keratinocytes (NEKs) are isolated from normal skin tissue and cultured in Promocell complete culture medium (Keratinocyte Growth Medium 2, C-20011) at 37°C, 5% CO2, and subcultured every 5 days when the cells grow to near confluence.

[0044] Cell viability detection: Take NEKs cells in the best growth state, and perform routine treatment. The cell suspension density is adjusted to 8×10 4 -1×10 5Cells were seeded at a concentration of 100 μL per well of the cell suspension in a 96-well plate and incubated at 37°C in a 5% CO2 incubator. 50 μL of the sample solution was added to each well and incubated for 24 hours. A negative control group (sampling solution replaced with an equal amount of cell culture medium) and a blank control group (cell culture medium alone) were set up. Three replicate wells were set up for each experimental group. After 24 hours of incubation, 10 μL of CCK-8 reagent was added to each well and incubated for 2 hours. Absorbance was measured at 450 nm using a microplate reader, with a reference wavelength of 600 nm or higher.

[0045] ROS detection: NEKs cells in the best growth state were cultured with Promocell special culture medium, and the cell suspension density was adjusted to 8×10 4 -1×10 5 Cells were seeded in 96-well plates at a concentration of 100 μL per well at 37°C in a 5% CO2 incubator. Vitamin K3 was added to each well at a final concentration of 10 μmol / L. The experimental group received 50 μL of sample solution, the positive control group received 50 μL of vitamin E solution (cell culture medium, resulting in a final vitamin E concentration of 200 μmol / L), and the blank control group received 50 μL of cell culture medium. After 24 hours of incubation, 1 μL of ROS fluorescent colorimetric reagent (CellRox, Thermo) was added to each well and incubated for 4 hours. Fluorescence was measured using a microplate reader with an excitation wavelength of 485 nm and an emission wavelength of 520 nm.

[0046] Relative cell viability / % = (test group test value - blank group test value) / (negative group test value - blank group test value) × 100% The test results are expressed as mean values, as shown in Table 2.

[0047] ROS inhibition rate / %=(fluorescence intensity of blank group - fluorescence intensity of experimental group or positive group) / fluorescence intensity of blank group × 100% The test results are expressed as mean values, as shown in Table 3.

[0048] Table 2 Effects of the composition on NEKs activity Group Cell viability / % Negative group 100 Composition 1 98.42 Composition 2 99.73 Composition 3 99.64 Composition 1 98.19 Composition 2 98.76 Composition 3 97.42 Composition 4 97.98 Composition 5 98.51 Composition 6 99.36 According to the results in Table 2, the composition has little effect on the viability of NEKs cells. Based on this, the next step is to conduct ROS detection test.

[0049] Table 3 Effect of the composition on ROS inhibition rate Group ROS inhibition rate / % Composition 1 34.57 Composition 2 33.59 Composition 3 32.41 Composition 1 20.16 Composition 2 22.31 Composition 3 21.52 Composition 4 20.73 Composition 5 22.48 Composition 6 28.19 Vitamin E 45.73 As shown in Table 3, the results of compositions 1-3 show that composition 1 has the best ROS inhibition rate, so composition 1 is selected as the best embodiment.

[0050] From the ROS inhibition rate results of composition 1 and compositions ①-⑥, it can be seen that in the composition provided by the present application, there is a significant synergistic effect between the E. punctata bark extract, the A. integrifolia fruit extract, the R. chalceroides leaf cell extract, the M. alba fruit extract and the PDRN. Specifically, from the results of composition 1 and compositions ①-⑤, it can be seen that there is a certain synergistic effect between the E. punctata bark extract, the A. integrifolia fruit extract, the R. chalceroides leaf cell extract, the M. alba fruit extract and the PDRN in the present application; from the results of composition 1 and composition ⑥, it can be seen that the composition obtained by compounding the E. punctata bark extract, the A. integrifolia fruit extract, the R. chalceroides leaf cell extract, the M. alba fruit extract and the PDRN in the quality ratio defined in the present application has better ROS inhibition rate effect.

[0051] Test 2: Human safety test 2-1. Human skin patch test Tested substances: serum 1-3, serum ①.

[0052] Selection of subjects: healthy volunteers aged 21-57 years old were selected, voluntarily participated and signed the informed consent, could complete the test during the test period, randomly divided into groups, and 30 subjects were set in each group.

[0053] Experimental method: qualified patch equipment was selected, and the closed patch test method was used. 1 g of the tested substance was placed in the patch equipment and evenly coated, and then the low-sensitization adhesive tape was applied to the flexor of the forearm of the subject. After 24 hours, the tested substance was removed, and the skin reaction was observed and recorded at 0.5, 24 and 48 hours after removal, respectively.

[0054] Result evaluation: the skin adverse reaction grading was determined according to the skin adverse reaction grading of human trial test in the “Cosmetic Safety Technology Specification” (2015 edition), and the grading standard is shown in Table 4, and the results are shown in Table 5.

[0055] Table 4 Skin adverse reaction grading standard Grade classification Phenomenon 0 level Skin has no reaction 1 level Skin appears pinkish red spots 2 level Skin appears red spots, infiltration and papules 3 level Skin appears red spots, edema, papules and blisters 4 level Skin appears red spots, edema and bullae Table 5 Results of human skin patch test

[0056] 2-2. Human application test for sensitive skin Tested substances: serum 1-3, serum ①.

[0057] Selection of subjects: healthy volunteers aged 21-44 years old were selected, who met the positive lactic acid stinging test, voluntarily participated and signed the informed consent, could complete the test during the test period, randomly divided into groups, and 30 subjects were set in each group.

[0058] Experimental method: Volunteers randomly divided the bilateral face into test side and control side, after cleansing, according to the product use method, the product was evenly applied to the facial skin of the test side, and the control side did not use any product. After 28 days of trial, the volunteers fed back the local skin reaction, and if there was adverse reaction, the dermatologist evaluated the adverse reaction.

[0059] Result evaluation: The skin adverse reaction grading was judged according to the skin adverse reaction grading of human trial test in “Cosmetic Safety Technology Specification” (2015 edition), and the grading standard was shown in table 4, and the results were shown in table 6.

[0060] Table 6 Results of human trial test

[0061] From the results of human skin patch test, during the test period, essence 1-3 and essence 1 were safe, and the subjects had no adverse reactions.

[0062] From the results of sensitive skin test, during the test period, no subjects had skin adverse reactions, indicating that essence 1-3 and essence 1 were safe, mild and non-irritating, and suitable for sensitive skin population.

[0063] 2-3: Moisturizing property test: Test material: essence 1-3, essence 1.

[0064] Selection of subjects: healthy volunteers aged 21-44 years old were selected, who met the positive lactic acid stinging test, voluntarily participated and signed the informed consent, could complete the test according to the requirements during the test period, and were randomly divided into groups, with 30 subjects in each group.

[0065] Test method: The subjects washed their forearms, sat quietly for 30 min in the set humidity environment, and selected a square test area with a side length of 3 cm on the left and right forearms of the subjects. The left arm was used as the test area of the test material, and the symmetric area of the right arm was used as the blank control. The moisture content of each test site was detected by Corneometer CM825, repeated 3 times, and the average value was recorded.

[0066] The calculation formula of skin hydration rate is: Hydration rate = (test detection value-blank control detection value) / blank control detection value x 100% After using the sample for 2 hours, the average skin hydration rate of the subjects was shown in table 7.

[0067] Table 7 Effect of essence on skin hydration rate Group Hydration rate / % Serum 1 43.72 Serum 2 41.53 Serum 3 42.35 Serum 1 33.42 From the data in Table 7, it can be seen that the serums 1-3 have a significant effect of improving skin hydration. Compared with the results of serum 1 and serum 1, it can be seen that the serum prepared by the preparation method of the application has a more significant effect of improving skin hydration.

[0068] The above-described embodiments are part of, but not all of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

Claims

1. A moisturizing antioxidant composition containing a variegated bellflower tree extract and PDNR, characterized in that: The composition comprises a variegated bellflower tree bark extract, an Annona mume fruit extract, a desert rose leaf cell extract, a cornus officinalis fruit extract, and PDRN in a mass ratio of 1:0.8-1:2-3:1-2:1-1.

5.

2. The moisturizing antioxidant composition containing the Campanula variegata extract and PDNR according to claim 1, characterized in that: The composition comprises a variegated bellflower tree bark extract, an Annona mume fruit extract, a desert rose leaf cell extract, a cornus officinalis fruit extract, and PDRN in a mass ratio of 1:0.9:2.5:1.5:1.

3.

3. Use of the moisturizing and antioxidant composition containing the Campanula variegata extract and PDNR according to claim 1 or 2 in the preparation of cosmetics with moisturizing and antioxidant effects.

4. The application according to claim 3, characterized in that The dosage form of the cosmetic is any one of a face cream and an essence.

5. An essence with moisturizing and anti-oxidation effects, characterized in that: The essence contains 4-6 wt % of the moisturizing antioxidant composition containing the variegated bellflower tree extract and PDNR according to claim 1 or 2.

6. The essence with moisturizing and anti-oxidation effects according to claim 5, characterized in that: The essence also contains the following components in percentage by weight: Dipotassium glycyrrhizate: 0.1-0.3wt%; Hydroxypropyl methylcellulose: 0.5-1.5wt%; Deionized water was added to make up to 100 wt%.

7. A method for preparing the essence with moisturizing and antioxidant effects according to claim 6, characterized in that: The following steps are involved: A1: Mix 2 / 3 of the amount of deionized water with the variegated bellflower bark extract, Annona mume fruit extract, Rosa deserticola leaf cell extract, Cornus officinalis fruit extract, dipotassium glycyrrhizate, and hydroxypropyl methylcellulose at 50-60°C, and cool to 2-8°C to obtain a mixed solution A. A2: Mix PDRN and the remaining deionized water at 2-8°C to obtain mixed solution B. A3: Mix the mixture A and the mixture B at 2-8°C to obtain the essence, seal it, and store it at 2-8°C away from light.

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