Composition containing Lysimachia congestiflora Hemsl. extract

Abstract

The Lily of the Valley extract according to the present invention inhibits tyrosinase activity, reduces the total amount of melanin, thereby exhibiting a whitening effect; promotes collagen synthesis in skin fibroblasts, inhibits collagenase activity, thereby improving wrinkles; increases skin elasticity; and exhibits an anti-glycation effect by suppressing glycation. It also promotes hyaluronic acid synthesis, thereby exhibiting a skin moisturizing effect; and improves skin texture by improving rough skin. It also exhibits a soothing effect against irritation and stinging, itching, heat, etc., and can therefore be usefully used in the production of cosmetics, medicines, or foods.

Description

Technical Field

[0001] The present invention relates to a composition containing Takemayu extract.

Background Art

[0002] Desiring fair and clean skin is a common desire. The color and brightness of the skin are genetically determined by the concentration and distribution of melanin in human skin, but are also affected by environmental or physiological conditions such as solar ultraviolet rays, fatigue or stress. Melanin is produced from tyrosine, an amino acid, through the catalytic action of an enzyme called tyrosinase, which is sequentially changed to DOPA and dopaquinone, and then through a non-enzymatic oxidation reaction. Although the pathway by which melanin is produced is known, the cause of the activation of tyrosinase in the mechanism that induces melanin synthesis has not yet been clarified.

[0003] On the other hand, generally known skin-whitening components include substances that inhibit tyrosinase enzyme activity, such as kojic acid or arbutin, hydroquinone, vitamin C (L-ascorbic acid) or their derivatives, and extracts of various plants. These can not only brighten the skin tone and achieve skin whitening by inhibiting the synthesis of melanin pigments, but also improve skin hyperpigmentation disorders such as freckles and chloasma caused by ultraviolet rays, hormones or genetics. However, when applied to the skin, there are problems such as restrictions on the amount of use due to safety issues such as irritation and redness, or little effect and no substantial effect can be expected.

[0004] Collagen is also a major matrix protein produced by skin fibroblasts and exists in the extracellular matrix. Its important functions include the mechanical firmness of the skin, the resistance and binding force of connective tissue, the support of cell adhesion, and the induction of cell division and differentiation (during the growth of an organism or the healing of a wound). Such collagen is known to decrease due to aging and photoaging caused by ultraviolet radiation, which is closely related to the formation of wrinkles on the skin. In recent years, while extensive research on skin aging has been developing, the important functions of collagen in the skin have become clear.

[0005] Active ingredients that promote collagen synthesis and show an effect of improving wrinkles are known. For example, retinoic acid, TGF (transforming growth factor), proteins derived from animal placenta, Betulinic acid, and chlorella extract are known as substances that promote collagen synthesis. However, the above-mentioned active ingredients have problems such as limitations in the amount of use due to safety issues such as irritation and redness when applied to the skin, or the effect is small and it is impossible to expect an effect of substantially promoting collagen synthesis in the skin and improving the skin function.

[0006] Glycation generally refers to a reaction in which simple sugars such as glucose or fructose form covalent bonds with proteins or fats without the involvement of enzymes. The accumulation of advanced glycation end products changes proteins into a hard and more brittle state. Glycated collagen in the extracellular matrix of the dermis causes the loss of skin elasticity and promotes the formation of wrinkles by preventing collagen from forming an appropriate structure. In addition, the final glycation products generated by glycation are brownish substances, which are considered to be the causative agents of the yellowing of skin color as skin aging progresses. It is known that the more specific advanced glycation end products accumulate, the less reflected light is reflected by the skin. As substances that suppress glycation, aminoguanidine, pyridoxamine, aspirin, etc. are known, but these substances have problems such as restrictions on the amount of use due to safety issues for the skin, or little effect and practically no expected effect.

[0007] The skin is composed of three layers: the epidermis, the dermis, and subcutaneous fat. Among these, the dermis, which exists inside the epidermis, plays the most important role in terms of skin aging, and thus has become a focus of intensive research in terms of skin beauty. The constituent fibers that play an important role in maintaining the structure of the dermis include cytokeratin, integrin, laminin, and collagen. Such fibrous constituent fibers basically constitute the framework of the dermis and play a role in maintaining healthy skin. Among these, cytokeratin, integrin, and laminin exist at the dermo-epidermal junction where the epidermis and the dermis are connected, and play a role in regulating the strength of the connection between the epidermis and the dermis. Collagen is generally present inside the dermis and is known to play a role in maintaining the structure and elasticity of the entire skin. In particular, in the case of collagen, when a normal structure is not generated, it is known to be significantly weaker than the normal type in terms of strength and elasticity and is not useful for maintaining the structure of the dermis. Therefore, many studies have been conducted to promote the synthesis of each constituent fiber to improve the texture of the skin and analyze the effects on the skin.

[0008] However, in conventional cosmetic compositions, only an increase in the synthetic amount of each constituent fiber has been confirmed, and no research has been conducted on the substantial effect of improving skin texture. Therefore, there is a need to develop a substance that has no skin irritation and has an effect of improving skin texture.

[0009] In addition, there is an urgent need to develop a component that is safe for living organisms, has stable active ingredients, and has excellent activity compared to existing substances having effects such as skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, and / or skin texture improvement.

Summary of the Invention

Problems to be Solved by the Invention

[0010] An object of the present invention is to provide a cosmetic composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, which contains Takemakuri extract as an active ingredient.

[0011] Another object of the present invention is to provide a topical skin preparation for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, which contains Takemakuri extract as an active ingredient.

[0012] An object of the present invention is to provide a pharmaceutical composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, which contains Takemakuri extract as an active ingredient.

[0013] An object of the present invention is to provide a food composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, which contains Takemakuri extract as an active ingredient.

[0014] An object of the present invention is to provide a method for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, which includes a step of treating the skin with the Takemakuri extract.

[0015] An object of the present invention is to provide the use of Lilium hansonii extract for producing a cosmetic composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement.

[0016] The above object and other objects of the present invention can all be solved by the present invention described in detail below.

Means for Solving the Problems

[0017] The inventors of the present invention have confirmed that Lilium hansonii extract not only shows a whitening effect by reducing the total amount of melanin, promotes collagen synthesis in skin fibroblasts, inhibits collagenase activity to improve wrinkles, enhances elasticity, shows a skin calming effect, shows an anti-glycation effect of suppressing glycation, but also promotes the synthesis of hyaluronic acid to show a skin moisturizing effect and can show a skin texture improvement effect by improving rough skin, and thus completed the present invention.

[0018] Therefore, the present invention relates to the use of Lilium hansonii extract for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization and skin texture improvement.

[0019] Hereinafter, the configuration of the present invention will be described in detail.

[0020] The present invention provides a composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or skin texture improvement, which contains Lilium hansonii extract as an active ingredient.

[0021] In the present invention, "Lilium hansonii" is also called Lilium amabile var. hansonii, is a perennial herb belonging to the genus Lilium in the order Liliales and the family Liliaceae, and is known to be distributed in Ulleungdo Island and the northeastern area in Korea.

[0022] In the present invention, the Lysimachia congestiflora is not limited to a particular variety, and cultivated or commercially available Lysimachia congestiflora may be used. Further, all parts of Lysimachia congestiflora may be used for the Lysimachia congestiflora extract, and there is no limitation on the extraction site. The production of the Lysimachia congestiflora extract is not limited by the form of the plant body, and the plant body includes all those that have undergone a processing process such as drying. For example, the Lysimachia congestiflora in the present invention may be the whole herb, root, stem, leaf, or flower of Lysimachia congestiflora, and preferably, it may be the whole herb, root, or flower of Lysimachia congestiflora.

[0023] In the present invention, the "extract" includes the extract obtained by extraction treatment, the diluted or concentrated solution of the extract, the dried product obtained by drying the extract, the crude or purified product of the extract, or all dosage forms of the extract that can be formed using the extract itself and the extract, such as a mixture thereof. The Lysimachia congestiflora extract of the present invention may be extracted from natural, hybrid, or variant plants of Lysimachia congestiflora, and extraction is also possible from plant tissue cultures.

[0024] In the extraction of the present invention, the extraction method is not particularly limited, and extraction may be carried out by a method commonly used in the art. Non-limiting examples of the extraction method include solvent extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc. These may be carried out alone or in combination of two or more methods.

[0025] In the present invention, the drying of the extract may be carried out by a known method within a range where useful components in the collected plant are not destroyed. For example, it may be carried out by a natural drying method in the shade. Also, crushing is sufficient if it is crushed to an extent that useful components of the plant can be sufficiently extracted in the subsequent extraction process, and it can be powdered. The drying and crushing steps may be carried out in a changed order or repeatedly as necessary.

[0026] In the present invention, the type of extraction solvent used to extract Anemone rivularis var. flore-pleno is not particularly limited, and any solvent known in the art may be used. In the present invention, the extract is obtained by extraction with water, a lower alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof. Further, non-limiting examples of the extraction solvent include water, lower alcohols having 1 to 6 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol, polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol, hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane, or mixtures thereof may be used. Specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. At this time, when two or more solvents are used in combination, the mixing ratio between the solvents is not particularly limited.

[0027] In the present invention, more specifically, ethanol, ethyl acetate, water, dichloromethane, butanol, hexane, etc. may be used as the solvent for extracting Anemone rivularis var. flore-pleno. The Anemone rivularis var. flore-pleno extract extracted with the solvent may be produced as a dry extract by vacuum distillation followed by freeze-drying or spray-drying. In the following examples, an Anemone rivularis var. flore-pleno extract was produced using a mixed solvent of water and ethanol as the extraction solvent, and this was filtered and concentrated under reduced pressure for use.

[0028] The extraction may be carried out by applying a method of extracting at an extraction temperature of 10 to 80°C, specifically 15 to 50°C, for 2 hours to 30 days, specifically 12 hours to 18 days, using a solvent having a weight 1 to 30 times, specifically 2 to 10 times, the weight of the dried Anemone rivularis var. flore-pleno. The process may include obtaining a liquid crude extract by continuously extracting 1 to 5 times including the drying and the crushed material.

[0029] In the present invention, the extract may be used as it is, or dried using freeze-drying, hot air drying, spray drying, etc. after filtering with a filter such as nylon or filter paper to remove floating solid particles, or filtering using a freeze-filtration method.

[0030] The liquid crude extract can be separated from the dried and crushed plant material by a method such as vacuum filtration and then undergo a concentration or drying process. For example, it may be a concentrated solution obtained by vacuum rotary concentration of the liquid crude extract at 20 to 100°C, preferably 30 to 70°C, or the liquid extract can be dried to obtain a powdered extract. The extract thus concentrated or powdered may be dissolved in water, alcohol, DMSO (dimethyl sulfoxide) or a mixed solvent thereof and used as needed.

[0031] In the present invention, the active ingredient may be a fraction of the Takeshima lily extract.

[0032] In the present invention, the term "fraction" of the terms used means the result obtained by performing fractionation to separate a specific component or group of specific components from a mixture containing various components.

[0033] In the present invention, the fractionation method for obtaining the fraction is not particularly limited and may be performed by a usual method of use in the technical field. Non-limiting examples of the fractionation method include a method of treating a Takeshima lily extract obtained by extracting Takeshima lily with a predetermined solvent to obtain a fraction from the extract.

[0034] In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol, and non-polar solvents such as hexane, ethyl acetate, chloroform, dichloromethane, and butanol. These may be used alone or in combination of two or more. When alcohol is used among the fractionation solvents, an alcohol having 1 to 6 carbon atoms may be used.

[0035] In the present invention, the "skin whitening effect" means not only brightening the skin tone by inhibiting the synthesis of melanin pigment, but also improving skin hyperpigmentation such as freckles and chloasma caused by ultraviolet rays, hormones or genetics.

[0036] In the present invention, the "wrinkle improvement effect" means suppressing or inhibiting the generation of wrinkles on the skin, or relaxing the wrinkles that have already been generated.

[0037] In the present invention, the "elasticity enhancing effect" means suppressing or inhibiting the loss of skin elasticity by increasing the elasticity of the skin, or relaxing the elasticity that has already decreased.

[0038] In the present invention, the "skin soothing effect" means an effect of relieving or improving skin irritation symptoms caused by external stimuli, and restoring the skin symptoms or skin troubles caused by external stimuli to their original state. The skin irritation means that symptoms such as erythema, itching, tingling or heat sensation occur on the skin due to external stimuli, and may be induced by skin photosensitivity or allergy. More specifically, the skin soothing effect may be to relieve skin symptoms or skin troubles caused by external stimuli.

[0039] In the present invention, the "anti-glycation effect" refers to preventing or suppressing the glycation phenomenon in which glucose existing in cells reacts with proteins and the protein functions in the body decline. In vivo glycation, a Schiff base, which is an initial glycation product, is formed by the non-enzymatic reaction between the amino group of proteins in the skin and tissues and reducing sugars, and is rearranged to generate a more stable Amadori product. Subsequently, as time passes, when the Amadori product is exposed to reactive oxygen species, it undergoes various stages such as cleavage, cross-linking, aggregation, and the formation of carbonyl groups, and finally, advanced glycation end products (AGEs) are formed. The formed advanced glycation product-protein bond is very stable and an irreversible reaction, so it continuously increases with age, creating skin that is not transparent and has lost its elasticity. Furthermore, if the accumulation of advanced glycation end products continues, various skin problems may occur. When skin problems occur due to the accumulation of advanced glycation end products, there may be phenomena such as the skin not becoming clear but looking yellowish due to the progression of glycation, the skin becoming hard and not soft, and unevenness occurring partially due to the deposition of advanced glycation end products. In this regard, the anti-glycation effect in the present invention may also include suppressing or preventing skin problems caused by the progression of glycation and the accumulation of advanced glycation end products, improving skin tone, and improving the texture of the skin.

[0040] In the present invention, the "skin moisturizing effect" refers to inhibiting or suppressing the reduction of skin moisture, or increasing the skin moisture content to smooth the skin surface and impart gloss.

[0041] In the present invention, the "skin texture improving effect" refers to smoothing the skin surface, imparting luster, and improving the skin tone brightly by suppressing or inhibiting the roughening of the skin surface due to the effects of aging, stress, etc.

[0042] On the one hand, as used herein, the term "contained as an active ingredient" means containing an amount sufficient to achieve the aforementioned skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturizing, or skin texture improvement activities of the Takeshima lily extract.

[0043] Therefore, the present invention provides a cosmetic composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturizing, or skin texture improvement, which contains the Takeshima lily extract as an active ingredient.

[0044] When the composition is used as a cosmetic composition, it may be manufactured in the form of a general emulsifier form or solubilizer form. For example, it may have the dosage forms of lotions such as softening lotion or nutritive lotion, emulsions such as facial lotion or body lotion, creams such as nutritive cream, moisture cream or eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation in liquid, solid or spray form, powder, makeup removers such as cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap, body wash and other detergents.

[0045] In addition, the Takeshima lily extract may further contain additives commonly used in the cosmetic field, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic emulsifiers, non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic activators, lipophilic activators or lipid vesicles. The additives may include all natural additives or synthetic additives.

[0046] The natural additive means components such as organic agricultural raw materials, plants and plant-derived raw materials, animals and animal-derived raw materials, minerals and mineral-derived raw materials, and water. As an example, it may be one or more additives selected from the group consisting of a humectant, an ultraviolet blocker, a neutralizing agent, a fragrance, a preservative, an antioxidant, a thickening agent, a viscosity modifier, a film-forming agent, and a pigment.

[0047] In the present invention, the natural additive means that it is obtained from natural substances or natural materials, or the components derived from natural substances or natural materials are simply modified, or it includes synthetic components among the components derived from nature, and it means excluding synthetic components synthesized by artificial methods that are not components derived from nature.

[0048] The natural raw material may, as an example, mean a cosmetic raw material of an organic agriculture standard and an eco-certification grade or a natural-derived grade equivalent thereto determined by the state.

[0049] The eco-certification standards refer to the certification standards for compositions composed of components that have undergone eco-cultivation and processing while excluding synthetic processes. Representative eco-certifications include Ecocert in France, Cosmos in Europe, USDA (US Department of Agriculture) in the United States, BDIH (Association of German Industries and Trading Firms) in Germany, and JAS (Japanese Association of Standard) in Japan. In line with the consumer needs that prefer recent eco-products, various countries have developed diverse eco-certification systems to certify the safety of components or products. Although there are partial differences in the detailed numerical values and ranges for each country, in terms of the primary components of raw materials and ingredients, there is a global unity in the scope of eco-certification within a large framework. For natural-origin natural components, they are included in the scope of eco-organic agricultural components. For other semi-processed ingredient categories, they are classified into PPAI (Physically Processed Agro Ingredient) and CPAI (Chemically Processed Agro Ingredient), and certifications such as Ecocert, Cosmos, or USDA are only granted to raw materials that meet these standards.

[0050] In the present invention, the organic agriculture and eco-certified raw materials and ingredients defined by the state refer to cosmetic raw materials and compositions that are composed only of components that comply with or can comply with the organic agricultural standards and eco-certification standards defined by the state and can meet the organic agriculture and eco-grades defined by the state. For example, it means being composed only of components that comply with or can comply with the standards defined by the Chinese government or the organic agriculture and eco-certification standards recognized in China.

[0051] In the present invention, the synthetic additive means a raw material that is not of natural origin and has undergone a chemical synthesis process.

[0052] Each of the components contained in the cosmetic composition according to the present invention may preferably be contained in the cosmetic composition of the present invention within a range not exceeding the maximum usage amount specified in the "Cosmetics Safety and Technical Standards" defined by the Chinese government.

[0053] When the Takemakusa lily extract is commercialized as a cosmetic, in the case of wash-off type cosmetics such as makeup removers or cleansers where the active ingredient stays on the skin for a short period, it may contain a relatively high concentration of the Takemakusa lily extract. On the other hand, in the case of leave-on type cosmetics such as lotions, emulsions, creams or essences where the active ingredient stays on the skin for a long time, it may contain a lower concentration of the Takemakusa lily extract compared to wash-off type cosmetics. Without being limited thereto, in one specific example of the present invention, the composition may contain the Takemakusa lily extract in an amount of 0.0001 parts by weight to 10 parts by weight (preferably 0.0001 parts by weight to 5 parts by weight) based on the total weight of the composition. When the composition of the present invention contains the Takemakusa lily extract in an amount less than 0.0001 parts by weight, sufficient skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturizing or skin texture improvement effects cannot be expected. When it contains more than 10 parts by weight, unnecessary reactions such as allergies may occur, or problems may occur with skin safety. This is to prevent such situations.

[0054] The present invention also provides a topical skin preparation for improving skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturizing or skin texture improvement, containing the Takemakusa lily extract as an active ingredient.

[0055] When using the Takeshima lily extract as a topical skin preparation, it may further contain adjuvants used in the field of dermatology, such as fatty substances, organic solvents, solubilizers, thickeners, and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic emulsifiers, non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic activators, lipophilic activators, or lipid vesicles, etc. Any other components commonly used in topical skin preparations. Also, the above components may be introduced in amounts commonly used in the field of dermatology.

[0056] When the Takeshima lily extract is used as a topical skin preparation, although not limited thereto, it may be in a dosage form such as an ointment, patch, gel, cream, or aerosol.

[0057] Furthermore, the present invention provides a pharmaceutical composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization, or skin texture improvement, containing the Takeshima lily extract as an active ingredient.

[0058] The pharmaceutical composition may be in the form of a solution, suspension, or emulsion in an oil or aqueous medium, or in the form of an extract, powder, granule, tablet, or capsule.

[0059] In addition, the composition may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may contain known skin whitening, wrinkle improvement, elasticity enhancement, skin soothing, anti-glycation, skin moisturizing, and skin texture improving ingredients. Furthermore, when containing skin whitening, wrinkle improvement, elasticity enhancement, skin soothing, anti-glycation, skin moisturizing, and skin texture improving ingredients, the effects of skin whitening, wrinkle improvement, elasticity enhancement, skin soothing, anti-glycation, skin moisturizing, and skin texture improvement of the composition of the present invention can be further enhanced. When adding the above ingredients, the skin safety by combined use, the ease of formulation, and the stability of the active ingredient are considered. In a specific example of the present invention, the composition may further contain one or more components selected from the group consisting of substances that inhibit tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, vitamin C and their derivatives, and various plant extracts as skin whitening ingredients known in the art. The additional ingredients may be contained in an amount of 0.0001 part by weight to 10 parts by weight based on the total weight of the composition, and the above content range can be adjusted according to requirements such as skin safety and the ease of formulation of the above Takeshima lily extract.

[0060] In addition, the composition of the present invention may further contain a pharmaceutically acceptable carrier.

[0061] The pharmaceutically acceptable carrier may contain many components such as buffer solutions, sterile water for injection, normal saline or phosphate buffered saline, sucrose, histidine, salts, and polysorbate.

[0062] The composition of the present invention may be administered orally or parenterally, and may be in the form of general pharmaceutical preparations, for example, administered in various dosage forms of oral and parenteral during clinical administration. However, when formulating, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants commonly used.

[0063] Solid dosage forms for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations may be prepared by mixing at least one or more excipients, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc., into the pharmaceutical composition of the present invention.

[0064] In addition to simple excipients, lubricants such as magnesium, stearate, talc, etc. are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, such as wetting agents, sweeteners, fragrances, preservatives, etc., may also be included.

[0065] Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As non-aqueous solvents and suspension solvents, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, etc. may be used. As the base of suppositories, witepsol, macrogol, tween 61, cocoa butter, laurin fat, glycerogelatin, etc. may be used.

[0066] In the present invention, the "effective amount" means the amount of the extract that can whiten the skin, improve wrinkles, increase elasticity, soothe skin irritation, suppress glycation, impart moisture to the skin, or improve the skin texture. When the composition of the present invention contains the effective amount of the Takashima lily extract, it can provide preferable skin whitening effect, wrinkle improvement effect, elasticity enhancement effect, skin soothing effect, anti-glycation effect, skin moisturizing effect, and skin texture improvement effect. The effective amount of the Takashima lily extract contained in the composition of the present invention varies depending on the form in which the composition is commercialized, the method by which the compound is applied to the skin, and the time it stays on the skin. For example, when the composition is commercialized in a pharmaceutical dosage form, it may contain the Takashima lily extract at a higher concentration than when it is commercialized as a cosmetic routinely applied to the skin. Therefore, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the Takashima lily extract, and it may be administered 1 to 6 times a day.

[0067] The composition of the present invention may be used alone or in combination with methods such as surgery, radiotherapy, hormone therapy, chemotherapy, and the use of biological response modifiers.

[0068] The present invention also provides a quasi-drug for skin whitening, wrinkle improvement, elasticity enhancement, skin soothing, anti-glycation, skin moisturizing, or skin texture improvement, which contains the Takashima lily extract as an active ingredient.

[0069] In the present invention, the "quasi-drug" may further contain a pharmaceutically acceptable carrier, excipient, or diluent, in addition to containing the Takashima lily extract as an active ingredient. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, and may include, for example, a filler, bulking agent, binder, wetting agent, disintegrant, surfactant, lubricant, sweetener, fragrance, or preservative, but is not limited thereto.

[0070] The quasi-drug may be, for example, a disinfectant cleaner, shower foam, ointment solution, wet tissue, coating agent, etc., and preferably may be manufactured as a semi-solid preparation such as an external ointment or lotion, but is not limited thereto. The formulation method, dosage, usage method, constituent components, etc. of the quasi-drug may be appropriately selected from ordinary techniques known in the technical field.

[0071] The present invention also provides a food composition for whitening the skin, improving wrinkles, enhancing elasticity, calming the skin, anti-glycation, moisturizing the skin or improving skin texture, containing the Takeshima lily extract as an active ingredient.

[0072] The food dosage form means a food in which the Takeshima lily extract is added to food materials such as beverages, teas, spices, gums, confectioneries, etc., or a food manufactured by encapsulation, powderization, suspension, etc.

[0073] Since the food dosage form can be taken daily, high effects of skin whitening, wrinkle improvement, elasticity increase, skin calming, anti-glycation, skin moisturization and skin texture improvement can be expected, so it is very useful.

[0074] When the Takeshima lily extract is used as a food additive, the Takeshima lily extract may be added as it is, or used together with other foods or food components, and may be appropriately used by ordinary methods. The mixing amount of the active ingredient may be appropriately determined according to its purpose of use (prevention, health or therapeutic treatment). Generally, when manufacturing a food or beverage, the composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw materials. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health regulation, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

[0075] There are no particular restrictions on the types of the foods. Examples of the foods to which the substance can be added include meats, sausages, bread, chocolate, candies, snacks, confectioneries, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, drinking water, tea, drink agents, alcoholic beverages, and vitamin complexes, etc., and all health foods in the ordinary sense are included.

[0076] When the food dosage form is a beverage, various flavoring agents or natural carbohydrates, etc. may be contained as additional components like ordinary beverages. The natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As sweeteners, natural sweeteners such as thaumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrates is generally about 0.01 - 0.04 g, preferably about 0.02 - 0.03 g per 100 mL of the composition of the present invention.

[0077] In addition to the above, the food dosage form may contain various nutritional agents, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the food dosage form may contain pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not very important, but it is generally selected from the range of 0.01 - 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

[0078] In addition, the present invention provides a method for whitening the skin, improving wrinkles, enhancing elasticity, calming the skin, anti - glycation, moisturizing the skin, or improving skin texture, which includes the step of treating the skin with the extract of *Erythronium japonicum* Miq.

[0079] The present invention also provides the use of the Takemakuyuri extract for producing a cosmetic composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or pore refinement.

Effects of the Invention

[0080] The Takemakuyuri extract according to the present invention reduces the total amount of melanin, inhibits tyrosinase activity to exhibit a whitening effect, promotes collagen synthesis in skin fibroblasts, inhibits collagenase activity to improve wrinkles and enhance skin elasticity, exhibits a skin calming effect against irritation, stinging, itching, heat sensation, etc., shows an anti-glycation effect of suppressing glycation, promotes the synthesis of hyaluronic acid to exhibit a skin moisturizing effect, and shows a pore refinement effect by improving rough skin, so that the Takemakuyuri extract can be usefully used in the production of cosmetics, pharmaceuticals or foods.

Brief Description of the Drawings

[0081]

Figure 1

Modes for Carrying Out the Invention

[0082] Hereinafter, the present application will be described in detail by way of examples. However, the protection scope of the present application is not limited by the examples described below.

Examples

[0083] <Examples 1 to 3> Production of Takemakuyuri Extract

[0084] The whole herb (Example 1), roots (Example 2), and flowers (Example 3) of Lilium hansonii were prepared. An aqueous ethanol solution of 70% corresponding to 10 times the mass of each was added, and extraction was carried out at room temperature for 3 days, followed by filtration under reduced pressure. The filtered extract was concentrated and dried using a rotary evaporator (Buchi, Switzerland) to produce a Lilium hansonii extract.

[0085] <Comparative Examples 1 - 3> Preparation of extracts of Lilium plants

[0086] For each of the whole herbs of Lilium candidum (Comparative Example 1), Lilium Siberia (Comparative Example 2), and Lilium martagon (Comparative Example 3), an aqueous ethanol solution of 70% corresponding to 10 times the mass was added, and extraction was carried out at room temperature for 3 days, followed by filtration under reduced pressure. The filtered extract was concentrated and dried using a rotary evaporator (Buchi, Switzerland) to produce each extract.

[0087] <Experimental Example 1> Confirmation of the effect of improving skin wrinkles and enhancing elasticity

[0088] 1) Effect of increasing total collagen amount

[0089] Three types of Lilium hansonii extracts and extracts of Lilium plants were added to the culture medium of human-derived fibroblasts, and the effect of increasing the total collagen amount at the cell level was compared.

[0090] The measurement of the increased total collagen amount was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). The concentrations (μg / ml) of the Lilium hansonii extracts prepared in Examples 1 - 3 and the extracts of Lilium plants prepared in Comparative Examples 1 - 3 before the experiment were changed, and the cytotoxicity was evaluated for human-derived fibroblasts. A concentration without cytotoxicity was selected (100 μg / ml), and the degree of increase in the total collagen amount was evaluated.

[0091] After adding each sample to the culture medium of human fibroblasts and culturing for 1 day, a culture solution was obtained, and the degree of increase in the total amount of collagen at each concentration was measured at 450 nm using a spectrophotometer with a PICP EIA kit. For comparison of the effects, the degree of increase in the total amount of collagen was measured in the same manner for the culture medium of fibroblasts without any addition (control group) and the sample added with vitamin C at a final concentration of 52.8 μg / ml. The total amount of collagen was measured as UV absorbance, and the increase rate of the total amount of collagen was calculated as the ratio of the relative total amount of collagen to the control group. The results were summarized in Table 1 below.

[0092]

Table 1

[0093] As can be seen from the results in Table 1 above, the Takeshima lily extract is excellent in promoting collagen synthesis and increasing the total amount of collagen, and shows a collagen total amount increasing effect similar to or better than that of vitamin C and lily plant extracts, which are known to have the effect of increasing the total amount of collagen at the test concentrations.

[0094] 2) Collagenase activity inhibitory effect

[0095] The collagenase activity inhibitory effect was confirmed as follows for the Takeshima lily extract or three kinds of lily plant extracts.

[0096] Fibroblasts, which are normal human skin cells, were inoculated into a 24-well microplate at a cell number of 2.5×10 4 per well and cultured for 24 hours under the conditions of 10% serum DMEM medium and 37°C. Then, the 10% serum DMEM medium was removed, and after washing once with a phosphate buffer solution, the fibroblasts were further cultured for 30 minutes in a serum-free DMEM medium added with Examples 1 to 3 and Comparative Examples 1 to 3. At this time, as a control group, the fibroblasts were further cultured for 30 minutes in a serum-free DMEM medium not containing the extract.

[0097] After 30 minutes of sample treatment, fibroblasts were stimulated with 10 ng / ml of TNF-α (tumor necrosis factor-α), a substance known to generate MMP-1, which is a collagenase, and then cultured for 24 hours. At this time, the TNF-α untreated group and the treated group were set as the untreated group without TNF-α treatment and the treated group with TNF-α treatment, respectively, among the control groups that did not contain the Takeshima lily extract of the present invention.

[0098] The supernatant of each well was collected, and the amount (ng / ml) of newly synthesized MMP-1 was measured using an MMP-1 analysis kit (Amersham, USA). The collagenase activity inhibition rate was calculated as the MMP-1 production inhibition rate (%) according to the following formula 1. The results are shown in Table 2 below.

[0099] In the following formula 1, the amount of MMP-1 in the negative control group means the amount of MMP-1 in the TNF-α untreated group, the amount of MMP-1 in the positive control group means the amount of MMP-1 in the TNF-α treated group, and the amount of MMP-1 in the experimental group means the group to which Examples 1 to 3 and Comparative Examples 1 to 3 were added at each concentration.

[0100]

Formula

[0101] (X = the amount of MMP-1 in the experimental group, Y = the amount of MMP-1 in the positive control group, Z = the amount of MMP-1 in the negative control group)

[0102]

Table 2

[0103] As can be seen from the results in Table 2 above, when evaluating Examples 1 to 3 and Comparative Examples 1 to 3, it can be seen that at the same concentration, the Takeshima lily extract has an excellent inhibitory ability against MMP-1, which is a collagenase, at a level about 2 to 3 times that of the lily plant extract.

[0104] <Experimental Example 2> Confirmation of skin whitening effect

[0105] 1) Tyrosinase inhibitory effect

[0106] The tyrosinase enzyme was extracted from mushrooms and the one commercially available from SIGMA was used. First, tyrosine as a substrate was dissolved in distilled water to make a 0.3 mg / ml solution, and 1.0 ml of this solution was put into each test tube. Then, 1.0 ml of potassium-phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water were added thereto. 0.2 ml of the sample solutions prepared by mixing Examples 1 to 3 and Comparative Examples 1 to 3 of the present invention in an ethanol solution at a final concentration of 100 μg / ml respectively were put into the reaction solution, and then reacted in a 37°C constant temperature bath for 10 minutes. At this time, for the control group, only 0.2 ml of ethanol solution was put instead of the sample solution, and vitamin C was used as the positive control group. 0.1 ml of a 2500 units / ml tyrosinase solution was put into this reaction solution, and reacted again in a 37°C constant temperature bath for 10 minutes. The test tubes containing this reaction solution were put into ice water to rapidly cool and stop the reaction, and the absorbance at a wavelength of 475 nm was measured with a photoelectric spectrophotometer, and the results are shown in Table 3 below. Each tyrosinase inhibitory effect was calculated by the following formula (2).

[0107]

Formula

[0108]

Table 3

[0109] As shown in Table 3, the tyrosinase inhibitory effect by the extract of <Takeshima lily> can be confirmed. In particular, Examples 1 to 3 had a higher inhibitory effect than ascorbic acid (vitamin C) known as a tyrosinase inhibitor at a concentration of 100 μg / ml, and the whitening effect was very excellent.

[0110] 2) Confirmation of the effect of reducing the total amount of melanin

[0111] Examples 1 to 3 and Comparative Examples 1 to 3 were each added to the culture medium of mouse melanoma cells (B-16 mouse melanoma cell), and the whitening effect at the cellular level was experimented on.

[0112] Each extract was experimented with a final concentration of 100 μg / ml in the culture medium, and arbutin, which is the control group, was also added to the medium to be 100 μg / ml. Each was treated with B-16 melanoma cells and cultured for 3 days.

[0113] Thereafter, the cultured cells were treated with trypsin, detached from the culture vessel, centrifuged, and then melanin was extracted. The detached cells were added with 1 ml of sodium hydroxide solution (1N), boiled for 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

[0114] The amount of the melanin was measured by the method shown by the absorbance per unit cell number (10 6 cells), calculated by the inhibition rate (%) with respect to the total amount of relative melanin to the control group, and the results were summarized in Table 4.

[0115] [Table 4]

[0116] As can be seen from the results in Table 4 above, the whitening effect was confirmed in all examples with respect to the Takemashigure extract. In particular, in Examples 1 to 3, when compared with arbutin whose whitening effect is known, the melanin inhibitory effect is even more excellent at the same concentration, and it can also be confirmed that when compared with the lily plant extract which is a comparative example, it shows an effect more than twice as excellent.

[0117] <Experimental Example 3> Confirmation of the moisturizing effect of the skin - 1

[0118] 1) Confirmation of the effect of enhancing hyaluronic acid synthesis

[0119] Human dermal fibroblasts were cultured in DMEM medium containing 10% fetal bovine serum at a density of 1×10 5 cells / mL, dispensed into 24-well plates at 500 μL per well, and cultured for 18 hours. Then, the samples were diluted in DMEM medium without fetal bovine serum, and each sample was added at the following concentrations. As a positive control group, epidermal growth factor (EGF) known to promote hyaluronic acid production was treated at a concentration of 10 ppb. After culturing for 24 hours, the cell culture supernatant was collected, and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems).

[0120] Also, based on the hyaluronic acid concentration of the non-additive control group as a reference (100%), the hyaluronic acid concentrations of the experimental groups treated with each sample were quantified and shown in Table 5 below.

[0121]

Table 5

[0122] As shown in Table 5 above, it was confirmed that hyaluronic acid synthesis increased in all examples with respect to the Takeshima lily extract. In particular, Examples 1 to 3 showed even better efficacy compared to the positive control group, despite being at a higher concentration than the positive control group, and it can be seen that the effect is very excellent compared to the comparative examples which are extracts of other lily plants.

[0123] <Production Example 1> Production of essence containing Takeshima lily extract

[0124] To confirm the skin-improving effect of the Takeshima lily extract produced in Examples 1 to 3 above, an essence was produced according to the composition in Table 6 below.

[0125]

Table 6

[0126] <Experimental Example 4> Confirmation of Skin Moisturizing Effect - 2

[0127] 1) Change in Skin Moisture Content Before and After Using Essence

[0128] To experiment on the skin moisturizing effect, skin evaluation was conducted using the essence produced in Production Example 1. As evaluation subjects, 20 women aged 30 to 40 were randomly selected in groups of 5 each, and after applying Essences A to D to the entire face respectively, the skin moisture content was measured 2 weeks later. Specifically, after washing the faces of the 20 subjects with soap, the skin was adapted to constant temperature and humidity conditions (temperature 20 ± 2°C, relative humidity 40 ± 2%). Using a corneometer (Corneometer, CM825), the skin surface capacitance was repeatedly measured 3 times. The average results are shown in Table 7.

[0129]

Table 7

[0130] As shown in Table 7 above, when using essences (A to C) containing Anemone rivularis Buch.-Ham. ex DC. extract for 2 weeks, it was confirmed that the moisture content on the skin surface was significantly higher than that of the control group (D), and it was confirmed that Anemone rivularis Buch.-Ham. ex DC. extract has an excellent moisturizing effect.

[0131] <Experimental Example 5> Confirmation of Skin Texture Improvement Effect

[0132] 1) Confirmation of Skin Texture Improvement Effect on Human Skin

[0133] A sensory evaluation was conducted to confirm the effect of the essence produced in Production Example 1 on improving the texture of the skin. After recruiting 40 women in their 30s to 50s, 10 people were randomly selected and each was applied with Essences A to D twice a day on the entire face for 4 weeks. After 4 weeks of use, scores from 1 to 10 were assigned according to the degree of improvement in skin roughness, and the effect of improving the skin texture was compared. At this time, the closer to 1 point, the less the effect of improving the skin texture, and the closer to 10 points, the better the effect of improving the skin texture. The average score for each is shown in Table 8 below.

[0134]

Table 8

[0135] As shown in Table 8 above, it can be seen that when using the essence containing the Gypsophila pacifica extract (A to C), the effect of improving the skin texture is even better than when using the one without the Gypsophila pacifica extract (D).

[0136] 2) Questionnaire evaluation of skin improvement degree

[0137] To confirm the skin improvement effect of Essence A of Production Example 1, a questionnaire evaluation was conducted. 11 women in their 30s to 50s were applied with A twice a day on the entire face for 4 weeks. After 4 weeks of use, a questionnaire sheet was created to evaluate the degree of skin improvement and is shown in Figure 1. In Figure 1, the percentage of subjects who answered "Yes" and "Very much so" is shown.

[0138] As shown in Figure 1, it was confirmed that more than 70% of the users who used Essence A of Production Example 1 containing the Gypsophila pacifica extract felt an excellent effect of improving the skin texture.

[0139] <Experimental Example 6> Confirmation of anti-glycation effect

[0140] To confirm the anti-glycation efficacy, the glycation inhibition activity was measured using L-arginine and glucose.

[0141] First, 1 ML - arginine and 1 M glucose were dissolved using 1 M phosphate buffer solution (pH 7.4), and diluted with 1 M phosphate buffer solution to prepare a sample with a concentration of 100 μg / ml. After mixing 1 ML - arginine and 1 M phosphate buffer solution at a ratio of 1:4, 80 μl of the mixture was dispensed into each well of a 96 - well plate. Then, 100 μl each of the sample diluted to 100 μg / ml and 0.01 M aminoguanidine used as a positive control group were added thereto. After thoroughly mixing these samples, finally, glucose diluted with 1 M phosphate buffer solution to a final concentration of 0.1 M was added, and the mixture was reacted at 70°C for 4 hours. The absorbance of the 96 - well plate was measured at 420 nm using a spectrophotometer to measure the degree of glycation.

[0142] The Glycation experimental group of the following formula 3 was an experimental group in which glycation was induced by adding 1 ML - arginine and 1 M glucose. To measure the absorbance of the sample itself, only 1 ML - arginine and the sample were added without adding glucose, and the absorbance was measured at 420 nm.

[0143] The glycation inhibitory activity is determined by the following formula 3. The experiment was conducted three times each and shown as the average value.

[0144]

Equation

[0145]

Table 9

[0146] As shown in the results of Table 9 above, an anti - glycation effect was confirmed in all examples for the extract of Anemone rivularis Buch.-Ham. When compared with aminoguanidine, which is known as an anti - glycation substance, an anti - glycation effect at a level similar to that of aminoguanidine was shown. Due to such an excellent anti - glycation effect of the extract of Anemone rivularis Buch.-Ham., it can be expected that skin troubles caused by advanced glycation end products will be improved.

[0147] <Experimental Example 7> Confirmation of Skin Soothing Effect

[0148] 1) Confirmation of Stimulation Relief Effect

[0149] To confirm the stimulation relief efficacy of the Anemone rivularis Buch.-Ham. extract, the inhibitory effect on PGE2 (Prostaglandin E2), a stimulation-related substance, was measured.

[0150] First, to measure prostaglandin E2 (PGE2), mouse macrophage RAW 264.7 Mouse macrophage cell line (KCLB no 40071, Korean Cell Line Bank, Seoul, Korea) was purchased and dispensed in 100 μl aliquots into a 96-well microplate at a cell seeding concentration of 2.5×10 6 cells / ml. As the medium, DMEM (Gibco BRL, Grand Island, NY, USA) medium supplemented with 10% FBS (GibcoBRL, Grand Island, NY, USA) and 1% penicillin / streptomycin (Thermo Scientific Hyclone, Waltham, MA, USA) was used, and the cells were cultured in an environment of 5% CO 2 , 37 °C. LPS (Lipopolysaccharide) (Sigma, St. Louis, MO, US) at 1 μg / ml was added to the cell culture solution in which the RAW 264.7 cells were dispensed to induce stimulation, and Examples 1 to 3 and Comparative Examples 1 to 3 were treated. Then, the cells were cultured for 24 hours, centrifuged at 900 rpm and 4 °C for 5 minutes, and the cell culture solution was collected to confirm the production amount of prostaglandin E2 (PGE2) produced in the culture solution. Prostaglandin E2 (PGE2) was measured according to the manufacturer's instructions using a commercially available PGE2 ELISA kit (MyBioSource Co., Ltd., San Diego, CA, USA).

[0151]

Table 10

[0152] As a result, in Examples 1 to 3 for the Takeshima lily extract, a reduction effect on the amount of PGE2 production was confirmed in all cases. That is, it was confirmed that the Takeshima lily extract can relieve skin irritation due to the PGE2 production inhibitory effect.

[0153] 2) Confirmation of the sedative effect on the burning sensation, itching, and heat sensation

[0154] After inducing irritation to confirm the skin sedative effect of the Takeshima lily extract, it was evaluated whether the burning sensation, itching, and heat sensation were sedated.

[0155] First, a chamber with an aqueous solution of 0.2% sodium lauryl sulfate (SLES) applied was attached to the arm sites of 15 healthy adult males and 15 adult females. After 8 hours, the pinch chamber was removed, and the burning sensation, itching, and heat sensation felt by the evaluators were evaluated according to the criteria shown in the following table. The evaluation criteria are as shown in Table 11 below.

[0156] <Skin Sedative Effect Evaluation Criteria>

[0157]

Table 11

[0158] Next, the burning sensation, itching, and heat sensation felt after applying Essences A to D manufactured earlier, which were randomly selected, once a day (at 9:00 am) for 3 days to the skin sites where SLES was applied were evaluated. The results are as follows.

[0159]

Table 12

[0160]

Table 13

[0161]

Table 14

[0162] As shown in Tables 12 to 14 above, it was confirmed that the degree of stinging, itching, and heat sensation after using Essences A to C containing the Takeshima lily extract was significantly reduced. On the other hand, in the case of Essence D that does not contain the Takeshima lily extract, it was confirmed that the difference between the 0th day and the 3rd day was very small.

Claims

1. A cosmetic composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or skin texture improvement, comprising an extract of Lilium hansonii as an active ingredient, wherein the extract of Lilium hansonii is an extract obtained by extracting Lilium hansonii with a mixed solvent of water and a lower alcohol having 1 to 6 carbon atoms.

2. The cosmetic composition according to Claim 1, wherein the extract of Lilium hansonii is contained in an amount of 0.0001 to 10 parts by weight based on 100 parts by weight of the composition.

3. A pharmaceutical composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or skin texture improvement, comprising an extract of Lilium hansonii as an active ingredient, wherein the extract of Lilium hansonii is an extract obtained by extracting Lilium hansonii with a mixed solvent of water and a lower alcohol having 1 to 6 carbon atoms.

4. A topical skin preparation for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or skin texture improvement, comprising an extract of Lilium hansonii as an active ingredient, wherein the extract of Lilium hansonii is an extract obtained by extracting Lilium hansonii with a mixed solvent of water and a lower alcohol having 1 to 6 carbon atoms.

5. A food composition for skin whitening, wrinkle improvement, elasticity enhancement, skin calming, anti-glycation, skin moisturization or skin texture improvement, comprising an extract of Lilium hansonii as an active ingredient, wherein the extract of Lilium hansonii is an extract obtained by extracting Lilium hansonii with a mixed solvent of water and a lower alcohol having 1 to 6 carbon atoms.

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