Oral composition

Abstract

To provide an oral composition containing charcoal with high versatility in order to effective utilize the charcoal.SOLUTION: An oral composition has charcoal and at least one selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, an amino acid, a peptide and a protein.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an oral composition containing charcoal and specific other materials.

[0002] Most of charcoal is a porous substance composed of carbon, oxygen, hydrogen, calcium, etc. in addition to carbon, and has the property of adsorbing various substances in its fine pores.

[0003] Charcoal is industrially used as a decolorizing agent (Patent Document 1) that adsorbs coloring components and a deodorizing agent (Patent Document 2) that adsorbs odor substances, and is used as an aid for filtration and purification. In recent years, supplements utilizing the effects of charcoal have attracted attention.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] However, when charcoal is used in an oral composition such as a supplement, the effects exerted by charcoal have not been sufficiently studied.

[0006] An object of the present invention is to provide an oral composition with high versatility for the purpose of effectively utilizing charcoal.

Means for Solving the Problems

[0007] As a result of intensive investigations and research on the functions of charcoal, the inventors of the present invention have found that by combining charcoal with 3-hydroxy-3-methylbutyric acid, creatine, amino acids or peptides (hereinafter also referred to as specific other materials), charcoal improves the undesirable taste such as bitterness of the specific other materials, improves the hardness when formulated into tablets, and exhibits an excellent muscle strengthening effect, thus leading to the present invention.

[0008] That is, the present invention is as follows. [1] An oral composition comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides. [2] An oral composition for muscle strengthening, comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides. [3] An oral composition comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides, wherein the taste of the other material is improved by the charcoal. [4] A tablet comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides.

Effects of the Invention

[0009] According to the present invention, by combining charcoal with specific other materials, an oral composition in which the taste of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides is improved by charcoal can be provided. Further, according to the present invention, a tablet with improved hardness can be provided. Furthermore, according to the present invention, an oral composition that can be expected to have a remarkable muscle strengthening effect can be provided.

Brief Description of the Drawings

[0010]

Figure 1

Figure 2

Figure 3

Mode for Carrying Out the Invention

[0011] The composition of the present invention is characterized by containing carbon and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides. The specific other materials used together with carbon may be used alone or in combination of two or more.

[0012] According to the present invention, a composition with improved taste derived from a specific other material can be obtained by combining carbon with a specific other material. Therefore, the composition of the present invention has high usability as an oral composition.

[0013] According to the present invention, when made into tablets by combining carbon with a specific other material, the hardness of the tablets is improved. Therefore, the composition of the present invention has high usability as tablets.

[0014] According to the present invention, a dramatic muscle strengthening effect is exerted by combining carbon with a specific other material. Therefore, the composition of the present invention has high usability as an oral composition for muscle strengthening.

[0015] Hereinafter, the materials used in the present invention will be described in detail.

[0016] [Carbon] Charcoal is obtained by treating plants, compounds, and synthetic resins under high-temperature conditions and is mainly used as fuel and as an aid for filtration and purification. There are no particular restrictions on the raw materials for charcoal that can be used in the present invention as long as they can be orally ingested. Examples include trees such as oaks, oaks, chestnuts, larch, red pine, and cedar used for Binchotan charcoal, bamboo, coconut shells, walnut shells, rice husks, etc. It is preferable to use bamboo, red pine, coconut shells, and rice husks, and bamboo is particularly preferable. As the charcoal, it is preferable to use activated charcoal obtained by carbonizing the raw materials and further activating them by high-temperature treatment. Since it exhibits excellent taste-improving effects, tablet hardness and stability-improving effects, and muscle-strengthening effects when combined with specific other materials, it is most preferable to use activated charcoal derived from bamboo. The activated charcoal is not particularly limited as long as its adsorption ability is activated. For example, in the case of commercially available products, those with notations of activated charcoal in the raw material specification sheets, etc. may be mentioned. The shape of the charcoal in the present invention is not limited as long as it is suitable for consumption. Examples include powder form, granular form, fibrous form, formed charcoal, etc., and powder form and granular form are preferable.

[0017] [Specific other materials] The composition of the present invention contains at least one (selected from 3-hydroxy-3-methylbutyric acid, creatine, amino acids, and peptides) as an essential component. Hereinafter, the specific other materials will be described in detail.

[0018] (3-Hydroxy-3-methylbutyric acid) 3-Hydroxy-3-methylbutyric acid (HMB) is a metabolite of the essential amino acid leucine and is synthesized in the human body. HMB in the present invention may be any physiologically available form of HMB, such as free acid and salts, as well as derivatives thereof. The form of HMB is not particularly limited, and examples include salts such as free acid, calcium salt, sodium salt, potassium salt, magnesium salt, esters, lactones, hydrates, etc. Among them, salts are preferred, and calcium salt (HMBCa) is particularly preferred because it exhibits excellent tablet hardness improvement effect and muscle strengthening effect when combined with charcoal. HMB in the oral composition of the present invention can be synthesized by known methods or commercially available products can be used. Commercially available products are not limited as long as they are applicable to foods.

[0019] (Creatine) Creatine is a kind of organic acid biosynthesized from arginine, glycine and methionine in the animal body. Creatine reacts with ATP and becomes creatine phosphate, which is a storage form of energy, by possessing an energy-rich phosphate group. Creatine phosphate synthesizes ATP by supplying a phosphate group when energy is insufficient and supplies energy. In the present invention, creatine may be any physiologically available form of creatine, such as free acid and salts, as well as derivatives thereof. The form of creatine is not particularly limited, and examples include salts such as free acid, calcium salt, sodium salt, potassium salt, magnesium salt, esters, lactones, hydrates, etc. Among them, creatine calcium salt, creatine monohydrate and tricreatine malate are preferred, and creatine monohydrate is particularly preferred because it exhibits excellent tablet hardness improvement effect and muscle strengthening effect when combined with charcoal. Creatine in the oral composition of the present invention can be synthesized by known methods or commercially available products can be used. Commercially available products are not limited as long as they are applicable to foods.

[0020] (Amino acid) An amino acid is an organic compound having functional groups of both an amino group and a carboxyl group, and includes amino acids contained as residues in proteins in the bodies of animals such as the human body, and amino acids and their salts that are not contained in proteins but exist naturally. In the present invention, for example, alanine, arginine, asparagine, aspartic acid, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, leucine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, cysteine, valine, cystine, hydroxyproline, citrulline, ornithine, etc. can be mentioned, and arginine, glutamine, isoleucine, leucine, citrulline, valine, ornithine are preferable, and valine, leucine, isoleucine are particularly preferable because they exhibit excellent tablet hardness improving action and muscle strengthening action when combined with carbon. Two or more kinds of amino acids may be used in combination. In the present invention, it may be obtained by using a method such as extraction from a natural material containing an amino acid, or an industrially produced amino acid may be used. Also, in the present invention, commercially available products can be used.

[0021] (Peptide) A peptide refers to a substance in which two or more amino acids are dehydrated and bonded between an amino group and a carboxyl group to form a peptide bond, and salts thereof. That is, the peptides in the present invention include, for example, oligopeptides having 10 or fewer amino acids, polypeptides having 11 or more amino acids, proteins, and the like. Examples of peptides include carnosine, anserine, and balenine, which are imidazole dipeptides, as well as kyotorphin, aspartame, glolin, valetine, pseudoproline, glycylglutamine, glycyltyrosine, alanylglutamine, glycylglycine, homanserine, glutathione, ophthamic acid, norophthamic acid, isoleucine-proline-proline (IPP), glypromate, leupeptin, eisenine, collagen, peptide hormones (melanostatin, thyrotropin-releasing hormone, growth hormone, insulin, glucagon, etc.), and in addition, animal proteins derived from livestock meats such as cattle, pigs, and chickens, fish, milk, eggs, etc., and their decomposed peptides, and plant proteins derived from soybeans, wheat, corn, peas, etc., and their decomposed peptides. In the present invention, peptides are preferably carnosine, anserine, balenine, collagen peptides, milk protein, soy protein, and collagen. Since they exhibit excellent tablet hardness improvement and muscle strengthening effects when combined with charcoal, it is particularly preferable to use carnosine, anserine, balenine, and milk protein. Two or more peptides may be used in combination. Also, industrially produced peptides may be used as the peptides of the present invention. Further, in the present invention, commercially available products can be used.

[0022] According to the present invention, by combining charcoal with a specific other material, the taste of the other material is improved by the charcoal. Therefore, by making the composition of the present invention, since a taste improvement effect by charcoal is expected, the utilization applications of charcoal can be expanded.

[0023] In addition, according to the present invention, when made into tablets by combining carbon with specific other materials, the hardness decreases. Therefore, by using the composition of the present invention, since an improvement effect in disintegration in the digestive tract is expected due to the decrease in the hardness of the tablets, the utilization applications of carbon can be expanded.

[0024] In addition, the composition of the present invention exhibits a dramatic muscle strengthening effect by blending carbon with specific other materials. For example, it can be used as so-called health foods such as pharmaceuticals (including quasi-drugs), foods for specified health use, food with nutrient function claims, foods with function claims, etc., for which efficacy display has been approved by a prescribed authority, as well as general foods, food additives, and the like. Therefore, since the composition of the present invention has high usability as an oral composition for muscle strengthening, the utilization applications of carbon can be expanded.

[0025] The composition of the present invention can be used as an oral composition for muscle strengthening used for muscle strengthening. Such an oral composition for muscle strengthening contains carbon and specific other materials, and is not particularly limited as long as it can be distinguished from other products as a product in terms of being used for muscle strengthening. For example, those in which the function of muscle strengthening is indicated in any of the main body, packaging, instruction manual, and promotional materials of the product according to the present invention are included in the scope of the present invention. Note that the oral composition for muscle strengthening of the present invention is not limited to those in which the components of the combination in the present invention (carbon and specific other materials) are indicated as active ingredients for muscle strengthening on the packaging of the product or the like. For example, it may not specify the active ingredient, or it may indicate only carbon or a specific other material as the active ingredient.

[0026] Specifically, the oral composition for muscle enhancement of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods. Among so-called health foods, examples include those labeled with "supporting the power to build muscles", "suppressing muscle breakdown", "enhancing muscle strength", "suppressing, maintaining, improving, and enhancing the decline of walking ability", "aiming to improve metabolism through muscle enhancement", "suppressing the decline of metabolism", "preventing and recovering from muscle fatigue", "suppressing and maintaining the decline of balance ability", "shaping up", "macho", "slim macho", "beautiful body", "preventing bedridden state", "preventing from becoming bedridden", "preventing falls", "aiming to improve metabolism through muscle enhancement", "maintaining muscle mass and muscle strength, etc.

[0027] Regarding the content of carbon and specific other materials (hereinafter sometimes referred to as the components of the present invention) in the composition of the present invention, it may be appropriately contained within the range where the effects are exhibited.

[0028] Specifically, when the oral composition of the present invention is a tablet, spherical, capsule-shaped supplement or pharmaceutical, it is preferable that the components of the present invention are contained in an amount of 5 to 99% by mass in terms of dry mass, more preferably 8 to 98% by mass, and particularly preferably 10 to 97% by mass. When the oral composition of the present invention is a chewable supplement or pharmaceutical, it is preferable that the components of the present invention are contained in an amount of 0.1 to 70% by mass in terms of dry mass, more preferably 0.5 to 60% by mass, and particularly preferably 1 to 50% by mass. In the case of powder-like, granular supplements or pharmaceuticals, it is preferable that the components of the present invention are contained in an amount of 0.1 to 90% by mass in terms of dry mass, more preferably 0.5 to 80% by mass, and particularly preferably 1 to 70% by mass. In the case of liquid supplements or pharmaceuticals, it is preferable that the components of the present invention are contained in an amount of 5 to 99% by mass in terms of dry mass, more preferably 8 to 98% by mass, and particularly preferably 10 to 97% by mass.

[0029] Although there is no particular limitation on the amount of carbon intake in the present invention, from the viewpoint of more significantly exerting the effects of the present invention (taste improvement, improvement of tablet hardness, and muscle strengthening) when combined with other materials, it is preferable to ingest carbon in an amount of 1 mg / day or more, more preferably 5 mg / day or more, and even more preferably 10 mg / day or more per adult per day. The upper limit is, for example, 50000 mg / day, preferably 30000 mg / day, and more preferably 10000 mg / day.

[0030] Also, although there is no particular limitation on the amount of HMB intake in the present invention, from the viewpoint of more significantly exerting the tablet hardness improvement effect and muscle strengthening effect when combined with other materials, it is preferable to ingest 100 mg / day or more, more preferably 200 mg / day or more, and even more preferably 300 mg / day or more per adult per day. The upper limit is, for example, 10000 mg / day, preferably 8000 mg / day, and more preferably 5000 mg / day. And although there is no particular limitation on the intake amounts of creatine, amino acids, and peptides of the present invention, it is preferable to ingest 0.001 mg / day or more, more preferably 0.005 mg / day or more, and even more preferably 0.01 mg / day or more per adult per day. The upper limit is, for example, 10000 mg / day, preferably 5000 mg / day, and more preferably 1000 mg / day. The oral composition of the present invention may be appropriately designed so that the daily intake amount is the above-mentioned intake amount, and it may be ingested once or divided into multiple times. For example, in the case of tablets, capsules, pills, or chewables, the number of intakes per day is 1 to 9 times, and it is sufficient if the total amount can be ingested as the above-mentioned intake amount. The oral composition of the present invention can be contained in one container or, for example, divided into 2 to 3 multiple containers as one-day supply so that the daily intake amount is the above-mentioned intake amount.

[0031] The blending mass ratio of carbon and HMB is preferably in the range of 1:0.01 to 5000, more preferably in the range of 1:0.1 to 3000, still more preferably in the range of 1:1 to 1000, and particularly preferably in the range of 1:3 to 300 from the viewpoint of more significantly exerting the excellent tablet hardness improving effect and muscle strengthening effect when combined. The blending mass ratio of carbon and creatine, amino acid or peptide is preferably in the range of 1:0.000001 to 30, more preferably in the range of 1:0.000003 to 10, still more preferably in the range of 1:0.000005 to 1, and particularly preferably in the range of 1:0.00001 to 0.1 from the viewpoint of more significantly exerting the excellent tablet hardness improving effect and muscle strengthening effect when combined.

[0032] In the case of tablet-shaped, round, capsule-shaped, chewable, powder-shaped, granular supplements and pharmaceuticals with respect to the total amount of the components of the present invention, it is preferable that carbon is contained in an amount of 0.0001 to 70% by mass in terms of dry mass, more preferably 0.001 to 50% by mass, and particularly preferably 0.01 to 30% by mass from the viewpoint of more significantly exerting the excellent tablet hardness improving effect and muscle strengthening effect when combined with other materials. In the case of liquid supplements and pharmaceuticals, it is preferable that carbon is contained in an amount of 0.000001 to 10% by mass in terms of dry mass, more preferably 0.00001 to 5% by mass, and particularly preferably 0.0001 to 3% by mass from the viewpoint of more significantly exerting the excellent tablet hardness improving effect and muscle strengthening effect when combined with other materials.

[0033] Examples of the form of the composition of the present invention include tablet form, capsule form, powder form, granule form, liquid form, granular form, rod form, plate form, block form, solid form, round form, paste form, cream form, caplet form, gel form, chewable form, stick form, etc. Among these, tablet form, capsule form, powder form, granule form, and liquid form are preferable, and tablet form and granule form are particularly preferable. Specifically, supplements, container-packed beverages filled in PET bottles, cans, bottles, etc., instant beverages to be dissolved in water (hot water), milk, fruit juice, etc. for drinking, and food additives can be exemplified. In particular, supplements and instant beverages are preferable in that they can be easily drunk and can enhance palatability.

[0034] When the composition of the present invention is made into tablets, pills, or chewables, it is preferable to add one or more of excipients, lubricants, and fluidizing agents to enhance moldability and improve the storage stability of the obtained tablet-shaped, pill-shaped, or chewable composition. In particular, the use of excipients and lubricants can further enhance storage stability. An excipient is added to improve the handling or molding of the composition or to facilitate administration. There are no particular restrictions on the excipients that can be used in the present invention. Examples include starches such as starch, pregelatinized starch, partially pregelatinized starch, and starch hydrolyzates, or their derivatives, crystalline cellulose, powdered cellulose, sugar alcohols, lactose, brewer's yeast, low-substituted hydroxypropyl cellulose, hydroxypropyl cellulose, refined sugar, light anhydrous silicic acid, calcium silicate, titanium oxide, precipitated calcium carbonate, reduced maltose, maltose, and the like. These may be used alone or in combination of two or more. In the present invention, from the viewpoints of moldability and storage stability, hydroxypropyl cellulose, powdered cellulose, pregelatinized starch, pregelatinized starch, sugar alcohol, reduced maltose, and maltose are preferably used as excipients. A lubricant is used to reduce the friction between the tablet press mortar and the tablet when compressing the powder for tablets and to prevent tableting problems such as sticking. There are no particular restrictions on the lubricants that can be used in the present invention as long as they can achieve the above object. Examples include stearic acid or its salts such as stearic acid, calcium stearate, and magnesium stearate, sodium stearyl fumarate, sucrose fatty acid ester, talc, polyethylene glycol, vegetable oils and fats, and hydrogenated oils. These may be used alone or in combination of two or more. In the present invention, from the viewpoints of moldability and storage stability, calcium stearate and sucrose fatty acid ester are preferably used as lubricants. A fluidizing agent is used to improve the fluidity of the mixed powder or granules. There are no particular restrictions on the fluidizing agents that can be used in the present invention. Examples include silicon dioxide, aluminum silicate, magnesium aluminum silicate, calcium phosphate, magnesium carbonate, magnesium oxide, and the like.These may be used alone or in combination of two or more. In the present invention, from the viewpoints of moldability and storage stability, silicon dioxide and magnesium carbonate are preferably used as the fluidizing agent. In the present invention, commercially available products can be used for all of the excipient, lubricant, and fluidizing agent.

[0035] The composition of the present invention can be produced by a known method by adding other components other than the components of the present invention, if necessary. Examples of other components other than the components of the present invention include vitamins such as water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), fat-soluble vitamins (vitamin A, D, E, K); minerals such as calcium, magnesium, phosphorus, iron; sulfur-containing compounds contained in taurine, garlic, etc.; flavonoids or flavonoid classes such as hesperidin, quercetin; animal fats and oils; vegetable fats and oils; pulverized products or extracts of animals and plants, etc.

Examples

[0036] Hereinafter, the present invention will be described based on examples, but the present invention is not limited to these examples, and the present invention can take various aspects as long as the problems of the present invention can be solved.

[0037] The following were used as the test substances in Tests 1 to 2. · As the charcoal, powder of bamboo-derived activated carbon (commercially available product) was used. · As HMB or its derivative, HMBCa (commercially available product) was used. · As creatine, creatine monohydrate (commercially available product) was used. · As the amino acid, L-isoleucine (commercially available product) was used. · As the peptide, collagen peptide (commercially available product) was used. · As the protein, soy protein (commercially available product) was used.

[0038] [Test 1: Sensory evaluation of oral composition] (1) Preparation of Samples Regarding the samples of Comparative Examples 1 to 5 and Examples 1 to 7 described in Table 1 below, after mixing the raw materials in the described compounding ratios, tablets with a weight of 300 mg per tablet and a tablet diameter of 9 mm were produced using a tableting machine. The units of the numerical values in the table are %.

[0039]

Table 1

[0040] (2) Evaluation of Samples As subjects, 5 healthy adults were randomly selected. For these 5 subjects, a questionnaire was conducted and a sensory evaluation was performed regarding the evaluation items in Table 2 below. Specifically, the samples of each example were evaluated based on any one of the comparative examples as a reference (0 point), and scores ranging from -4 to +4 were given respectively. Note that -4 points indicates the worst and +4 points indicates the best. For each evaluation item, the average score of the 5 subjects was calculated and is shown in Table 3 below. "Average" in Table 3 refers to the average score of the scores of each evaluation item. Note that the comparative example used as a reference for each example is as shown in Table 3 below.

[0041]

Table 2

[0042]

Table 3

[0043] (3) Discussion Regarding each comparative example and example, the evaluation results of palatability were discussed.

[0044] As shown in Table 3, Examples 1 to 5 in which carbon was combined with Comparative Examples 1 to 5 containing HMBCa, creatine, isoleucine, collagen peptide, or soy protein had improved bitterness, astringency, mildness, odor, and sweetness compared to Comparative Examples 1 to 5. Also, Examples 6 and 7 in which carbon was blended with the combination of HMBCa and soy protein and the combination of isoleucine and creatine also had improved bitterness, astringency, mildness, odor, and sweetness compared to Comparative Examples 2 and 3. Therefore, it was found that when carbon was combined with other materials of the present invention, a composition in which the taste of other materials was improved by carbon could be obtained.

[0045] [Test 2: Evaluation of Tablet Hardness] (1) Evaluation of Samples Examples 1 to 5 and Comparative Examples 1 to 5 prepared in Test 1 were evaluated using a tablet hardness tester (manufactured by Okada Seiko Co., Ltd.). The results are shown in Table 4.

[0046] [Table 4]

[0047] (2) Consideration For each of the comparative examples and examples, the evaluation results of the hardness of the tablets were considered.

[0048] Generally, it is known that the lower the hardness of a tablet, the better its disintegrability in the digestive tract. As shown in Table 4, Examples 1 to 5 in which HMBCa, creatine, isoleucine, collagen peptide, or soy protein was combined with carbon had a lower tablet hardness than Comparative Examples 1 to 5 containing only other materials. Therefore, by combining carbon with specific other materials, the hardness of the tablet can be lowered, and it can be expected that a tablet with good disintegrability in the digestive tract and excellent immediate effect can be obtained.

[0049] The following were used as test substances in Tests 3 to 4. · As carbon, powdered activated carbon derived from bamboo (commercially available product) was used. · As HMB or its derivative, HMBCa (commercially available product) was used. · As creatine, creatine monohydrate (commercial product) was used. · As the amino acid, L-leucine (commercial product) was used. · As the peptide, an imidazole dipeptide (a chicken extract containing 15% or more of anserine and carnosine; commercial product) was used. · As the protein, milk protein (commercial product) was used.

[0050] [Test 3: Evaluation of myoblast activation effect] (1) In a 37 °C, 5% CO2 incubator, using a 75 cm2 flask, a mouse myoblast cell line (C2C12) was cultured in DMEM containing 10% (V / V) FBS. (2) The cells suspended by trypsin treatment were seeded at a cell density of 10000 cells / well into each well of a collagen-coated 96-well plate from the 75 cm2 flask. (3) Pre-cultured for 24 hours in a 37 °C, 5% CO2 incubator. (4) 200 μL each of DMEM containing a test substance at a predetermined concentration shown in Table 5 below, prepared so that hydrogen peroxide (H2O2) was 7.5 μM, and containing 0.5% (V / V) DMSO - 10% (V / V) FBS, was added, and cultured in a 37 °C, 5% CO2 incubator for 20 hours. As a control, DMEM containing 0.5% (V / V) DMSO - 10% (V / V) FBS and not containing a test substance prepared so that H2O2 was 7.5 μM was used. The concentration unit in Table 5 is μg / mL. (5) After removing the medium, each well was washed once with 200 μL / well of PBS. Then, 150 μL / well of a Cell Counting Kit-8 (manufactured by Dojindo Laboratories) solution diluted 30-fold with DMEM was added. (6) After allowing the plate after addition to stand in a 37 °C, 5% CO2 incubator to develop color appropriately, 100 μL was dispensed from each well into a new 96-well plate, and the absorbance at 450 nm of each well was measured. Based on the obtained data, the cell viability (% of control) relative to the control was calculated according to the following formula (1).

[0051]

Table 5

[0052]

Number

[0053] The measurement results of the myoblast activation effect are shown in Fig. 1. The larger the numerical value, the higher the myoblast activation activity.

[0054] As shown in Fig. 1, under the condition of causing cell damage to myoblasts with H2O2, the composition combined with carbon, HMBCa, imidazole dipeptide, milk protein or creatine showed an excellent myoblast activation effect compared with each material alone. Therefore, it can be seen that the composition of the present invention has an excellent muscle strengthening effect.

[0055] [Test 4: Evaluation of myotube cell activation effect] (1) In a 37°C, 5% CO2 incubator, using a 75 cm2 flask, mouse myoblast cell line (C2C12) was cultured in DMEM containing 10% (V / V) FBS. (2) The cells suspended by trypsin treatment were seeded at a cell density of 5000 cells / well in each well of a 96-well plate coated with collagen from a 75 cm2 flask. (3) Pre-cultured for 48 hours in a 37°C, 5% CO2 incubator. (4) The medium was changed from DMEM containing 10% (V / V) FBS to a differentiation induction medium (DMEM containing 10% (V / V) FBS prepared so that the human insulin solution was 100 ng / mL and the horse serum was 2% (V / V)), and cultured in a 37°C, 5% CO2 incubator to induce differentiation into myotube cells. (5) 200 μL each of a differentiation induction medium containing a test substance at a predetermined concentration shown in Table 6 below, prepared such that H2O2 was 30 μM, and containing 0.5% (V / V) DMSO, was added and cultured in a 37 °C, 5% CO2 incubator for 20 hours. As a control, a differentiation induction medium containing 0.5% (V / V) DMSO and not containing a test substance prepared such that H2O2 was 30 μM was used. The concentration unit in Table 6 is μg / mL. (6) After removing the medium, each well was washed once with 200 μL / well of PBS. Subsequently, 150 μL / well of a Cell Counting Kit-8 (manufactured by Dojindo Laboratories) solution diluted 30-fold with DMEM was added. (7) After allowing the plate after addition to stand in a 37 °C, 5% CO2 incubator to develop color appropriately, 100 μL was dispensed from each well into a new 96-well plate, and the absorbance at 450 nm of each well was measured. Based on the obtained data, the cell activation activity (% of control) relative to the control was calculated according to the following formula (1).

[0056]

Table 6

[0057]

Equation

[0058] The measurement results of the myotube cell activation effect are shown in Figures 2 and 3. The larger the numerical value, the higher the myotube cell activation activity.

[0059] As shown in FIGS. 2 and 3, under the condition of causing cell damage to myotube cells with H2O2, a composition combined with carbon and one or more other materials selected from HMBCa, leucine, milk protein, and creatine showed an excellent myotube cell activation effect compared to each material alone. Also, Example 13 combining carbon and imidazole dipeptide showed an excellent myotube cell activation effect compared to Comparative Examples 11 and 13 using them alone. Therefore, it can be seen that the composition of the present invention has an excellent muscle strengthening effect.

[0060] [Production Example 1] (Production of tablets) Tablets (450 mg / granule) composed of the following components were formed using a tableting device. When the taste of the obtained tablets was evaluated, the unfavorable taste of other materials was improved. Also, when 9 granules of the obtained tablets were ingested per day, it was confirmed that they had a muscle strengthening effect.

[0061] Carbon powder (activated carbon from bamboo) 3% HMBCa 75% Creatine monohydrate 0.01% L-Valine 0.05% L-Isoleucine 0.05% L-Leucine 0.1% Chicken extract 0.001% (Containing 15% or more of anserine and carnosine) L-Glutamine 0.01% L-Ornithine hydrochloride 0.01% L-Arginine 0.01% L-Citrulline 0.01% Milk protein 0.001% Hydroxypropyl cellulose 4% Crystalline cellulose 10% Calcium stearate 2% Silicon dioxide 1% Reduced maltose the balance

[0062] [Production Example 2] (Production of tablets) A tablet (250 mg) composed of the following components was formed using a tableting machine. When the taste of the obtained tablet was evaluated, the unfavorable taste of other materials was improved. Also, when 5 tablets of the obtained tablet were ingested per day, it was confirmed to have a muscle-strengthening effect.

[0063] Charcoal powder (activated carbon from rice husk) 10% HMBCa 50% Creatine monohydrate 1% Collagen peptide 0.01% Ground black garlic powder 3% Lactic acid bacteria 0.1% Calcium stearate 2% Silicon dioxide 2% Crystalline cellulose 10% Reduced maltose the balance

[0064] [Production Example 3] (Production of Granules) After mixing the following components, a granulator was produced using a continuous fluidized granulation dryer. When the taste of the obtained granulator was evaluated, the unfavorable taste of other materials was improved. Also, when the obtained granulator was ingested, it was confirmed to have a muscle-strengthening effect.

[0065] Charcoal powder (activated carbon from bamboo) 5% HMBCa 50% Chicken extract 1% (Containing 15% or more of anserine and carnosine) Rice-derived glucosylceramide 2% Aspartame 0.1% Thiamine hydrochloride 0.5% Riboflavin 0.5% Vitamin B6 0.2% Cyanocobalamin 0.2% Flavor 0.2% Reduced palatinose 7% Sucrose fatty acid ester 5% Hydroxypropyl cellulose the balance

[0066] [Production Example 4] (Production of Instant Powdered Beverage) After mixing the following components, the mixture was subjected to a continuous fluidized granulation dryer to produce a powder for use in an instant powdered beverage. When the taste of the obtained powder dissolved in 100 mL of water was evaluated, the unfavorable taste of other ingredients was improved. In addition, when the obtained granule was ingested, it was confirmed to have a muscle strengthening effect. The ratio of the components is a value in terms of dry mass.

[0067] Charcoal powder (activated carbon from coconut shells) 3% Charcoal powder (activated carbon from Japanese red pine) 1% Charcoal powder (activated carbon from rice husks) 1% HMBCa 3% Soybean protein 1% Chia seed ground powder 3% Barley leaf extract powder 1% Aspartame 1% Erythritol 0.1% Sucralose 5% Malic acid 0.5% Vitamin mix 1% Dye preparation 0.5% Flavor 0.3% Polydextrose the balance

Industrial Applicability

[0068] According to the present invention, by combining charcoal with at least one selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids, and peptides, improvement in taste by charcoal and a hardness reducing effect of tablets are brought about, and the use of charcoal in foods and beverages can be expanded. Furthermore, as an effect of the present invention, an oral composition having an added value of a muscle strengthening effect can be provided.

Claims

【Claim 1】 An oral composition comprising carbon and one or more peptides selected from milk protein and soy protein (excluding (1) and (2) described below); (1) Foods such as natto, anko, tofu, aburaage, namaage, okara, yuba, soy milk, koji, kamaboko, ganmodoki, miso, soy sauce, natto, milk, condensed milk, powdered milk, fermented milk, butter, cheese, cream, ice cream or soft cream containing carbon, and foods made from the above foods. (2) A tablet-type oral administration composition comprising 65% by weight or more of particulate matter as an active ingredient and one or more additives, (a) The particulate matter does not exhibit solubility and swelling in water, has a strain rate of 2% or less when subjected to a pressure of 2 MPa, and a crushing strength of 5 MPa or more, (b) The tablet-type oral administration composition contains a total of 1% by weight or more of one or more additives for particulate preparations based on the tablet-type oral administration composition, and when 0.5 mL of an aqueous solution or aqueous dispersion of 1% by weight of the additive for particulate preparations is dropped onto a fluororesin plane and heated and dried, a thin film is formed. An oral administration composition characterized by this.).

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