Method for stratifying subjects

Abstract

To provide a method of stratifying subjects.SOLUTION: A method of stratifying subjects is provided, comprising classifying a subject into a group with or without plaque adhesion, and classifying the subject into a group with or without detected periodontal pathogens in an oral sample collected from the subject.SELECTED DRAWING: None

Description

Technical Field

[0001] The present disclosure relates to a method for stratifying subjects and the like. Specifically, it relates to a method for stratifying subjects for determining an oral hygiene product or an oral hygiene method. Further, the present disclosure relates to an oral hygiene product or the like for a subject stratified by the above method.

Background Art

[0002] Periodontal disease is an inflammatory disease caused by the infection of periodontal pathogenic bacteria in the periodontal tissue, and is roughly classified into gingivitis and periodontitis according to the degree of progression. Plaque caused by poor oral hygiene is considered to be one of the causes of periodontal disease, and since gingivitis occurs when oral hygiene actions are stopped, the importance of oral hygiene actions for removing plaque is shown.

[0003] In addition, it is considered important to select appropriate oral care according to the situation of the oral environment, such as the presence or absence and degree of periodontal disease, in order to improve and keep the oral environment clean.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] The progression from gingivitis to periodontitis is considered to occur as an extension of the persistence of gingival inflammation due to plaque adhesion. However, since oral bacteria such as periodontal pathogenic bacteria and biological factors (systemic diseases) are complexly involved, it is difficult to evaluate periodontal disease only based on the presence or absence of plaque adhesion, and there are problems in screening in the absence of a dental expert.

[0006] For example, when a subject suffering from periodontal disease performs thorough toothbrushing immediately before an oral examination, the plaque cleaning status will be good during the examination. However, it is unlikely that the degree of periodontal disease will be improved by such toothbrushing. As can be understood from this, it is often difficult to evaluate periodontal disease only based on the presence or absence of plaque adhesion. Therefore, the development of a simple diagnostic method for periodontal disease and the like is required (Patent Document 1).

[0007] An object of the present disclosure is to provide a method for stratifying subjects. Another object of the present disclosure is also to provide a method for stratifying subjects in order to determine an oral hygiene product or an oral hygiene method suitable for the presence or degree of periodontal disease.

Means for Solving the Problems

[0008] The inventors of the present invention have found that by classifying subjects by combining not only the presence or absence of plaque but also the presence or absence of periodontal pathogenic bacteria in saliva, it is possible to predict the presence or degree of periodontal disease, and have further improved it.

[0009] The present disclosure includes, for example, the subject matter described in the following items. Item 1. Classifying a subject into a group with plaque adhesion or a group without plaque adhesion, and Classifying the subject into a group with detection of periodontal pathogenic bacteria or a group without detection of periodontal pathogenic bacteria in an oral sample collected from the subject including A method for stratifying a subject. Item 2. Furthermore, the method according to Item 1, including comparing the age of the subject with a reference value and classifying the subject into a group equal to or higher than the reference value or a group lower than the reference value. Item 3. The method according to Item 1 or 2, which is used for determining an oral hygiene product or an oral hygiene method. Item 4. In the method according to any one of Items 1 to 3, An oral hygiene product for a subject classified into the group with plaque adhesion. Item 5. In the method according to any one of Items 1 to 3, without plaque adhesion, and an oral hygiene product for a subject classified into a group with detection of the periodontal pathogen. Item 6. In the method according to any one of Items 1 to 3, without plaque adhesion, and an oral hygiene product for a subject classified into a group without detection of the periodontal pathogen. Item 7. In the method according to Item 2 or 3, without plaque adhesion, with detection of the periodontal pathogen, and an oral hygiene product for a subject classified into a group with the age being equal to or higher than the reference value. Item 8. In the method according to Item 2 or 3, without plaque adhesion, with detection of the periodontal pathogen, and an oral hygiene product for a subject classified into a group with the age being less than the reference value. Item 9. A method for measuring the presence or absence or degree of periodontal disease of a subject using the presence or absence of plaque adhesion of the subject and the presence or absence of detection of periodontal pathogens in an oral sample collected from the subject as indicators, comprising stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of the periodontal pathogen The method includes.

Advantages of the Invention

[0010] A method for stratifying subjects is provided. Also, oral hygiene products for the stratified subjects are provided.

Brief Description of the Drawings

[0011]

Figure 1

Embodiments for Carrying Out the Invention

[0012] Hereinafter, each embodiment included in the present disclosure will be described in more detail. The present disclosure includes a method for stratifying subjects. In this specification, the stratification method may be referred to as "the stratification method of the present disclosure".

[0013] In this specification, "stratification" means classifying and extracting subjects into several groups.

[0014] The stratification method of the present disclosure includes classifying subjects into a group with plaque attachment or a group without plaque attachment. The stratification method of the present disclosure may include confirming the presence or absence of plaque attachment.

[0015] The subject is not particularly limited as long as it is a human. For example, it may be a healthy person, a human suffering from periodontal disease (periodontal disease patient), or a human suspected of suffering from periodontal disease.

[0016] In this specification, "plaque" means a mass of bacteria adhering to the surface of teeth. Plaque is sometimes referred to as dental plaque.

[0017] The method for confirming the presence or absence of plaque attachment is not particularly limited, and known methods can be adopted. For example, a method of rubbing the tooth surface with the tip of a periodontal probe or a dental explorer to determine the presence or absence of plaque attachment, a method of using a plaque staining solution to stain the tooth surface to determine the presence or absence of plaque attachment, a method of determining the presence or absence of plaque attachment by image diagnosis, a method of detecting plaque with a sensor, etc. can be mentioned. In addition, since plaque attachment occurs as a result of insufficient oral hygiene, it is already known that there is a relationship with the number of toothbrushing times and the use of interdental cleaning tools, and that gingival bleeding occurs when plaque attachment continues. Therefore, an oral hygiene behavior questionnaire (survey) or an examination for examining bleeding (occult blood) from the gingiva can also be used as a substitute for plaque attachment.

[0018] As the teeth to be checked for the presence or absence of plaque adhesion, for example, all teeth may be used, or the target teeth may be used. As the target teeth, for example, a total of six teeth such as the upper right 6, upper right 1, upper left 6, lower right 6, lower left 1, and lower left 6 may be used. The numbers correspond to the numbers shown in FIG. 1. Also, teeth that can be imaged with a smartphone or the like (mainly the anterior tooth region), or teeth that can be imaged with an intraoral camera may be used.

[0019] When plaque adhesion is not recognized or is below the detection limit by the method described above, it is classified into the group without plaque adhesion, and otherwise, it is classified into the group with plaque adhesion. Also, as shown in the examples described later, when evaluating the presence or absence of plaque by the Plaque Index (PlI), if the average value of the measured values of PlI is, for example, less than 0.5 (preferably less than 0.25, more preferably 0), it is classified into the group without plaque adhesion, and if it is 0.5 or more (preferably 0.25 or more, more preferably greater than 0), it is classified into the group with plaque adhesion.

[0020] The stratification method of the present disclosure includes classifying the subject into a group with or without detection of periodontal pathogenic bacteria in an oral sample collected from the subject. The stratification method of the present disclosure may include detecting periodontal pathogenic bacteria in an oral sample collected from the subject.

[0021] Examples of the oral sample include saliva, tongue coating, plaque, gingival crevicular fluid (GCF), etc. Among them, saliva is preferred. The oral sample may be separated by a filter paper, a centrifuge, or the like. Note that the method, conditions, etc. for storing the oral sample collected from the subject are not particularly limited and can be carried out according to a conventional method.

[0022] The method for collecting an oral sample from a subject is not particularly limited and may be performed according to a conventional method. Also, the collected oral sample may be used as it is, or after being lyophilized or otherwise preserved and then dissolving the lyophilized product in an appropriate solvent described below for use. Further, the collected oral sample may be frozen and stored as it is, or after being dissolved in an appropriate solvent described below and then frozen and stored, and thawed for use at the time of use.

[0023] Examples of the solvent for dissolving the oral sample include physiological saline, PBS, phosphate buffer, Tris buffer, borate buffer, TE buffer, Good Buffer, etc. These solvents may contain a surfactant; an enzyme activator such as a salt of a metal such as zinc or magnesium, etc.

[0024] The periodontal pathogen is not particularly limited as long as it is a known periodontal pathogen. For example, Porphyromonas gingivalis strains, etc. may be mentioned.

[0025] The method for detecting a periodontal pathogen is not particularly limited as long as it can detect a periodontal pathogen. For example, a method of extracting nucleic acid contained in an oral sample and detecting the nucleic acid derived from a periodontal pathogen contained in the nucleic acid, a method of measuring the enzyme activity of an enzyme specific to a periodontal pathogen (for example, protease, etc.), a method of measuring by an antigen-antibody reaction using an antibody that recognizes a periodontal pathogen, etc. may be mentioned. For these methods, known methods can be adopted.

[0026] Examples of the nucleic acid include DNA, RNA, etc. The method for extracting nucleic acid is not particularly limited, and a known method can be adopted.

[0027] As a method for detecting nucleic acids derived from periodontal pathogens contained in nucleic acids, there are no particular limitations, and known methods can be employed. For example, a method of amplifying 16S rRNA using primers for 16S rRNA amplification, sequencing (confirming) the sequence of the amplified nucleic acid, and confirming the presence or absence of nucleic acids derived from periodontal pathogens; a method of using primers that specifically amplify nucleic acids derived from periodontal pathogens to amplify the nucleic acids and detecting the amplified nucleic acids to confirm the presence or absence of nucleic acids derived from periodontal pathogens, etc. can be mentioned.

[0028] The primers may be prepared by known methods or may be commercially available.

[0029] As the amplification method, known methods can be adopted. For example, PCR etc. can be mentioned.

[0030] Regarding nucleic acids derived from periodontal pathogens, databases etc. can be referred to. As the database, the HOMD (Human Oral Microbial Database) database can be used.

[0031] As the sequencing method, known methods can be adopted. Also, when the amplified nucleic acid has a homology of 97% or more with periodontal pathogens registered in the database, the amplified nucleic acid is judged to be derived from the periodontal pathogen.

[0032] When periodontal pathogens are not detected or are below the detection lower limit by the method described above, they are classified into the group without detection of periodontal pathogens, and otherwise, they are classified into the group with detection of periodontal pathogens. The detection lower limit can be appropriately set according to the measurement method. For example, in a saliva sample, it may be about 10 2 cfu / mL, it may be about 10 3 cfu / mL, it may be about 10 4 cfu / mL, or it may be about 10 5 cfu / mL.

[0033] The stratification method of the present disclosure may further include comparing the age of the subject with a reference value and classifying the subject into a group above or below the reference value. For example, for a subject classified into a group with detection of periodontal pathogenic bacteria, the age of the subject may be compared with a reference value, and the subject may be classified into a group above or below the reference value.

[0034] As the reference value for comparison with the age of the subject, for example, it can be 40 years old, 50 years old, etc. Preferably, it is 40 years old.

[0035] The stratification method of the present disclosure can classify the subject into a group with plaque attachment and detection of periodontal pathogenic bacteria; a group with plaque attachment and no detection of periodontal pathogenic bacteria; a group without plaque attachment and detection of periodontal pathogenic bacteria; or a group without plaque attachment and no detection of periodontal pathogenic bacteria.

[0036] In addition, the stratification method of the present disclosure can classify the subject into a group with plaque attachment, detection of periodontal pathogenic bacteria, and age above the reference value; a group with plaque attachment, detection of periodontal pathogenic bacteria, and age below the reference value; a group with plaque attachment, no detection of periodontal pathogenic bacteria, and age above the reference value; a group with plaque attachment, no detection of periodontal pathogenic bacteria, and age below the reference value; a group without plaque attachment, detection of periodontal pathogenic bacteria, and age above the reference value; a group without plaque attachment, detection of periodontal pathogenic bacteria, and age below the reference value; a group without plaque attachment, no detection of periodontal pathogenic bacteria, and age above the reference value; or a group without plaque attachment, no detection of periodontal pathogenic bacteria, and age below the reference value.

[0037] According to the stratification method of the present disclosure, the subject can be stratified according to the presence or degree of periodontal disease.

[0038] Periodontal disease is roughly classified into gingivitis and periodontitis (mild periodontitis, severe periodontitis). As used herein, "gingivitis" means a condition in which plaque accumulates on the gingival margin, causing inflammation, swelling, and bleeding of the gingiva. As used herein, "periodontitis" refers to a condition in which gingivitis has further progressed, with plaque accumulating subgingivally, the alveolar bone being destroyed, the space between the tooth and the gingiva becoming deeper, and a periodontal pocket being formed. In this specification, when the depth of the periodontal pocket is 4 mm or more and less than 6 mm, it is defined as "mild periodontitis", and when the depth of the periodontal pocket is 6 mm or more, it is defined as "severe periodontitis".

[0039] According to the stratification method of the present disclosure, even in the case where a dental professional is absent or a dental check-up has not been performed, based on, for example, the collection of saliva and the like, and the analysis results and the captured oral images, etc., the subjects can be stratified according to the presence or absence or the degree of periodontal disease according to the presence or absence of plaque adhesion and the presence or absence of periodontal pathogens in the oral sample.

[0040] As shown in the examples described later, when there is plaque adhesion, the proportion of subjects without periodontal disease is low, and the proportion of subjects with gingivitis and mild periodontitis is high. Therefore, it is considered that plaque adhesion is involved in the onset (occurrence of inflammation) of periodontal disease. Also, as shown in the examples described later, when there is detection of periodontal pathogens, the proportion of subjects with gingivitis is low, and the proportion of subjects with periodontitis (mild periodontitis and severe periodontitis) is high. Therefore, it is considered that the presence or absence of periodontal pathogens is involved in the exacerbation of periodontal disease.

[0041] According to the stratification method of the present disclosure, in the group with plaque adhesion and detection of periodontal pathogens, the proportion of subjects with periodontitis (especially severe periodontitis) is higher compared to other groups. Also, in the group with plaque adhesion and detection of periodontal pathogens, the proportion of subjects with gingivitis is lower compared to other groups (more specifically, the group with plaque adhesion but no detection of periodontal pathogens). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group with plaque adhesion and detection of periodontal pathogens are highly likely to have periodontitis (especially severe periodontitis) compared to the subjects classified into other groups. According to the stratification method of the present disclosure, in the group with plaque adhesion but no detection of periodontal pathogens, the proportion of subjects with gingivitis is higher compared to other groups (more specifically, the group with plaque adhesion and detection of periodontal pathogens). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group with plaque adhesion but no detection of periodontal pathogens are highly likely to have gingivitis compared to the subjects classified into other groups. According to the stratification method of the present disclosure, in the group without plaque adhesion but with detection of periodontal pathogens, the proportion of subjects with periodontitis is higher compared to other groups (more specifically, the group without plaque adhesion and no detection of periodontal pathogens). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group without plaque adhesion but with detection of periodontal pathogens are highly likely to have periodontitis compared to the subjects classified into other groups (more specifically, the group without plaque adhesion and no detection of periodontal pathogens). According to the stratification method of the present disclosure, in the group without plaque adhesion and no detection of periodontal pathogens, the proportion of subjects without periodontal disease (healthy subjects) is high. Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group without plaque adhesion and no detection of periodontal pathogens are highly likely to be subjects without periodontal disease (healthy subjects).

[0042] According to the stratification method of the present disclosure, in the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value, the proportion of subjects with periodontitis (especially severe periodontitis) is higher compared to other groups. Also, in the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value, the proportion of subjects with gingivitis is lower compared to other groups (more specifically, the group with plaque adhesion but no detection of periodontal pathogenic bacteria, and the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age less than the reference value). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value have a higher likelihood of having periodontitis (especially severe periodontitis) compared to the subjects classified into other groups. According to the stratification method of the present disclosure, in the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age less than the reference value, the proportion of subjects with gingivitis is higher compared to other groups (more specifically, the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age less than the reference value have a higher likelihood of having gingivitis compared to the subjects classified into other groups (more specifically, the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value). According to the stratification method of the present disclosure, in the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value, the proportion of subjects with periodontitis is higher compared to other groups (more specifically, the group without plaque adhesion and no detection of periodontal pathogenic bacteria, and the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age less than the reference value). Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age equal to or higher than the reference value have a higher likelihood of having periodontitis compared to the subjects classified into other groups (more specifically, the group without plaque adhesion and no detection of periodontal pathogenic bacteria, and the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age less than the reference value). According to the stratification method of the present disclosure, in the group where there is no plaque adhesion, there is detection of periodontal pathogenic bacteria, and the age is less than the reference value, the proportion of subjects without periodontal disease (healthy subjects) is high. Therefore, according to the stratification method of the present disclosure, it can be predicted (determined) that the subjects classified into the group where there is no plaque adhesion, there is detection of periodontal pathogenic bacteria, and the age is less than the reference value are likely to be subjects without periodontal disease (healthy subjects).

[0043] According to the present disclosure, for example, even in the case of subjects classified into the group without plaque adhesion, it is possible to predict (determine) subjects suffering from periodontal disease or subjects with a high degree of such suffering. For example, as shown in the examples described later, when there is detection of periodontal pathogenic bacteria even without plaque adhesion, it can be predicted (determined) that there is a possibility of having periodontitis. Also, for example, as shown in the examples described later, when there is detection of periodontal pathogenic bacteria even without plaque adhesion and the age of the subject is equal to or greater than the reference value, it can be predicted (determined) that there is a possibility of having periodontitis. By using the age as the reference value, it is expected that the subjects can be stratified more accurately according to the presence or absence and degree of periodontal disease.

[0044] According to the present disclosure, for example, even in the case of subjects classified into the group with detection of periodontal pathogenic bacteria, it is possible to predict (determine) subjects without periodontal disease (healthy subjects). For example, as shown in the examples described later, even when there is detection of periodontal pathogenic bacteria, if there is no plaque adhesion, it can be predicted (determined) that the subject is likely to be a subject without periodontal disease (healthy subject). Also, for example, as shown in the examples described later, even when there is detection of periodontal pathogenic bacteria, if there is no plaque adhesion and the age of the subject is less than the reference value, it can be predicted (determined) that the subject is likely to be a subject without periodontal disease (healthy subject). By using the age as the reference value, it is expected that the subjects can be stratified more accurately according to the presence or absence and degree of periodontal disease.

[0045] According to the stratification method of the present disclosure, subjects can be stratified according to the presence or degree of periodontal disease. Therefore, the stratification method of the present disclosure can be suitably used to determine oral hygiene products or oral hygiene methods suitable for the subjects.

[0046] Examples of oral hygiene products include toothbrushes, interdental cleaners, ointments, pastes, pastas, gels, liquids, sprays, mouthwashes, liquid dentifrices, dentifrice pastes, gums, tablets, troches, drops, films, toothbrushing log devices for recording the number of toothbrushing times or toothbrushing time, light irradiation devices for sterilizing periodontal pathogenic bacteria, and the like. The oral hygiene products may be pharmaceuticals, quasi-drugs, foods, or the like. Examples of oral hygiene methods include toothbrushing methods, optimal usage methods of oral hygiene products, and the like. Although not particularly limited, more specifically, for example, the time for one toothbrushing (brushing) is set to 3 minutes or more, the number of toothbrushing times per day is set to 3 times or more, all teeth are evenly brushed to every corner, the size selection and usage method of interdental cleaners, and the like.

[0047] According to the present disclosure, oral hygiene products or oral hygiene methods suitable for the stratified subjects can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods for subjects classified into a group with plaque adhesion can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods for subjects classified into a group without plaque adhesion can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods for subjects classified into a group with detection of periodontal pathogenic bacteria can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods for subjects classified into a group without detection of periodontal pathogenic bacteria can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods for subjects classified into a group with plaque adhesion and detection of periodontal pathogenic bacteria can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having plaque adhesion and no detection of periodontal pathogenic bacteria can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having no plaque adhesion and having detection of periodontal pathogenic bacteria can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having no plaque adhesion and no detection of periodontal pathogenic bacteria can be provided.

[0048] Further, according to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having plaque adhesion, having detection of periodontal pathogenic bacteria, and having an age equal to or higher than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having plaque adhesion, having detection of periodontal pathogenic bacteria, and having an age less than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having plaque adhesion, having no detection of periodontal pathogenic bacteria, and having an age equal to or higher than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having plaque adhesion, having no detection of periodontal pathogenic bacteria, and having an age less than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having no plaque adhesion, having detection of periodontal pathogenic bacteria, and having an age equal to or higher than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having no plaque adhesion, having detection of periodontal pathogenic bacteria, and having an age less than a reference value can be provided. According to the present disclosure, for example, an oral hygiene product or an oral hygiene method for a subject classified into a group having no plaque adhesion, having no detection of periodontal pathogenic bacteria, and having an age equal to or higher than a reference value can be provided. According to the present disclosure, for example, oral hygiene products or oral hygiene methods can be provided for subjects classified into a group having no plaque adhesion, no detection of periodontal pathogenic bacteria, and an age less than a reference value.

[0049] The oral hygiene products may be, for example, for reducing plaque, suppressing plaque formation, killing periodontal pathogenic bacteria, suppressing the growth of periodontal pathogenic bacteria, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, and the like.

[0050] For subjects classified into a group having plaque adhesion, for example, reducing plaque, suppressing plaque formation, etc. are presumed to be effective for suppressing inflammation. As a means for reducing plaque, improvement of mechanical plaque removal (increase in the number of toothbrushing, use of interdental cleaners, etc.) is presumed to be particularly effective. For subjects classified into a group having no plaque adhesion and no detection of periodontal pathogenic bacteria, for example, maintaining the quality of plaque in a good state, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, etc. are presumed to be effective for preventing periodontal disease. Even for subjects classified into a group having no plaque adhesion, for subjects classified into a group having detection of periodontal pathogenic bacteria, for example, killing periodontal pathogenic bacteria, suppressing the growth of periodontal pathogenic bacteria, preventing the invasion of periodontal pathogenic bacteria into gingival cells, etc. are presumed to be effective for suppressing the progression of periodontal disease.

[0051] As oral hygiene products for subjects classified into a group having plaque adhesion, for example, those for reducing plaque, suppressing plaque formation, etc. may be used. As oral hygiene products for subjects classified into a group having no plaque adhesion, for example, those for killing periodontal pathogenic bacteria, suppressing the growth of periodontal pathogenic bacteria, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, etc. may be used. As oral hygiene products for subjects classified into a group having detection of periodontal pathogenic bacteria, for example, those for killing periodontal pathogenic bacteria, suppressing the growth of periodontal pathogenic bacteria, inhibiting the invasion of periodontal pathogenic bacteria into gingival cells, etc. may be used. For subjects classified into the group without detection of periodontal pathogenic bacteria, oral hygiene products may be, for example, those for suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, maintaining the oral bacterial flora, and the like. For subjects classified into the group with plaque adhesion and detection of periodontal pathogenic bacteria, oral hygiene products may be, for example, those for reducing plaque, suppressing plaque formation, killing periodontal pathogenic bacteria, inhibiting the growth of periodontal pathogenic bacteria, and the like. For subjects classified into the group with plaque adhesion and without detection of periodontal pathogenic bacteria, oral hygiene products may be, for example, those for reducing plaque, suppressing plaque formation, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, and the like. For subjects classified into the group without plaque adhesion and with detection of periodontal pathogenic bacteria, oral hygiene products may be, for example, those for suppressing plaque formation, killing periodontal pathogenic bacteria, inhibiting the growth of periodontal pathogenic bacteria, and the like. For subjects classified into the group without plaque adhesion and without detection of periodontal pathogenic bacteria, oral hygiene products may be, for example, those for suppressing plaque formation, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, and the like. For subjects classified into the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age above the reference value, oral hygiene products may be, for example, those for reducing plaque, suppressing plaque formation, killing periodontal pathogenic bacteria, inhibiting the growth of periodontal pathogenic bacteria, inhibiting the invasion of periodontal pathogenic bacteria into gingival cells, and the like. For subjects classified into the group with plaque adhesion, detection of periodontal pathogenic bacteria, and age below the reference value, oral hygiene products may be, for example, those for reducing plaque, suppressing plaque formation, suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, and the like. For subjects classified into the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age above the reference value, oral hygiene products may be, for example, those for killing periodontal pathogenic bacteria, inhibiting the growth of periodontal pathogenic bacteria, and the like. For subjects classified into the group without plaque adhesion, with detection of periodontal pathogenic bacteria, and age below the reference value, oral hygiene products may be, for example, those for suppressing the colonization of periodontal pathogenic bacteria, preventing the colonization of periodontal pathogenic bacteria, and the like.

[0052] The present disclosure can provide a program for realizing the above-described stratification method of the present disclosure. That is, the program causes a computer to have a first function of obtaining the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria in the oral sample obtained by the first function, it is a program for realizing a second function of stratifying the subject.

[0053] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and the stratification of a subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, the above-described description can be incorporated by reference.

[0054] The stratification result can be provided to the subject via a network, for example. Also, for example, oral hygiene products or oral hygiene methods for the subjects classified into the above-described groups can be presented to the subject via a network. For example, information such as the stratification result, oral hygiene products, or oral hygiene methods can be transmitted to the subject's smartphone, tablet terminal, etc. Also, for example, based on the stratification result, proposals, guidance, etc. of oral hygiene products or oral hygiene methods can be provided to the subject via a network.

[0055] The present disclosure also includes a method for measuring (predicting) the presence or absence or degree of periodontal disease of a subject. In this specification, this method may be referred to as "the method for measuring the presence or absence or degree of periodontal disease of the present disclosure".

[0056] The method for measuring the presence or absence or degree of periodontal disease of the present disclosure preferably uses the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample as indicators. The method for measuring the presence or absence or degree of periodontal disease of the present disclosure may further use age as an indicator.

[0057] The method for measuring the presence or absence or degree of periodontal disease according to the present disclosure preferably includes stratifying subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria. The method for measuring the presence or absence or degree of periodontal disease according to the present disclosure may further include stratifying subjects based on age.

[0058] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and methods for stratifying subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, etc., the description of the "stratification method of the present disclosure" described above can be incorporated by reference.

[0059] According to the method for measuring the presence or absence or degree of periodontal disease according to the present disclosure, based on the stratification result, the presence or absence or degree of periodontal disease of the subject can be measured (predicted).

[0060] The present disclosure can provide a program for realizing the method for measuring the presence or absence or degree of periodontal disease according to the present disclosure described above. That is, the program causes a computer to have a first function of acquiring the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria acquired by the first function, a second function of stratifying the subject, and based on the stratification result, a third function of measuring (predicting) the presence or absence or degree of periodontal disease of the subject.

[0061] The present disclosure also includes a method for assisting in the stratification of subjects according to the presence or absence or degree of periodontal disease. In this specification, this method may be referred to as the "method for assisting the stratification of the present disclosure".

[0062] The method for assisting the stratification of the present disclosure preferably uses the presence or absence of plaque adhesion of the subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample as indicators. The method for assisting the stratification of the present disclosure may further use age as an indicator.

[0063] The method for assisting stratification of the present disclosure preferably includes stratifying a subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogens. The method for assisting stratification of the present disclosure may further include stratifying a subject based on age.

[0064] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogens in an oral sample, and a method for stratifying a subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogens, etc., the description of the "stratification method of the present disclosure" described above can be incorporated by reference.

[0065] According to the method for assisting stratification of the present disclosure, it is possible to assist in stratifying a subject according to the presence or absence or degree of periodontal disease.

[0066] The present disclosure can provide a program for realizing the method for assisting stratification of the present disclosure described above. That is, the program causes a computer to have a first function of obtaining the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogens in an oral sample, and based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogens in the oral sample obtained by the first function, it is a program for realizing a second function of stratifying a subject.

[0067] The present disclosure also includes a method for assisting in determining (diagnosing) the presence or absence or degree of periodontal disease of a subject. In this specification, this method may be referred to as "a method for assisting in determining the presence or absence or degree of periodontal disease of the present disclosure".

[0068] The method for assisting in determining the presence or absence or degree of periodontal disease of the present disclosure preferably uses the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogens in an oral sample as indicators. The method for assisting in determining the presence or absence or degree of periodontal disease of the present disclosure may further use age as an indicator.

[0069] A method for assisting in determining the presence or absence or degree of periodontal disease in the present disclosure preferably includes stratifying subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria. The method for assisting in determining the presence or absence or degree of periodontal disease in the present disclosure may further include stratifying subjects based on age.

[0070] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and methods for stratifying subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, etc., the description of the "stratification method of the present disclosure" described above can be incorporated by reference.

[0071] According to the method for assisting in determining the presence or absence or degree of periodontal disease in the present disclosure, based on the stratification result, it is possible to assist in determining the presence or absence or degree of periodontal disease in the subject.

[0072] The present disclosure can provide a program for realizing the method for assisting in determining the presence or absence or degree of periodontal disease in the present disclosure described above. That is, the program causes a computer to have a first function of obtaining the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, a second function of stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria obtained by the first function, and a third function of determining the presence or absence or degree of periodontal disease in the subject based on the stratification result.

[0073] The present disclosure also includes a method for measuring (predicting) the risk of onset or exacerbation of periodontal disease in a subject. In this specification, this method may be referred to as the "method for measuring the risk of onset or exacerbation of periodontal disease in the present disclosure".

[0074] The method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure preferably uses the presence or absence of plaque adhesion in the subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample as indicators. The method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure may further use age as an indicator.

[0075] The method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure preferably includes stratifying the subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria. The method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure may further include stratifying the subjects based on age.

[0076] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and the method of stratifying subjects based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, etc., the description of the "stratification method according to the present disclosure" described above can be incorporated by reference.

[0077] According to the method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure, based on the stratification result, the risk of onset or exacerbation of periodontal disease in the subject can be measured (predicted).

[0078] The present disclosure can provide a program for realizing the method for measuring the risk of onset or exacerbation of periodontal disease according to the present disclosure described above. That is, the program causes a computer to have a first function of acquiring the presence or absence of plaque adhesion in a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, a second function of stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria acquired by the first function, and a third function of measuring (predicting) the risk of onset or exacerbation of periodontal disease in the subject based on the stratification result.

[0079] The present disclosure also includes a method for assisting in determining the risk of onset or exacerbation of periodontal disease in a subject. In this specification, this method may be referred to as "a method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure".

[0080] The method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure preferably uses the presence or absence of plaque adhesion in a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample as indicators. The method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure may further use age as an indicator.

[0081] The method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure preferably includes stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria. The method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure may further include stratifying the subject based on age.

[0082] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and the method of stratifying a subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, etc., the description of the "stratification method of the present disclosure" described above can be incorporated by reference.

[0083] According to the method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure, based on the stratification result, it is possible to assist in determining the risk of onset or exacerbation of periodontal disease in the subject.

[0084] The present disclosure can provide a program for realizing the method for assisting in determining the risk of onset or exacerbation of periodontal disease according to the present disclosure described above. That is, the program causes a computer to have a first function of acquiring the presence or absence of plaque adhesion in a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, a second function of stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria acquired by the first function, and a third function of determining the risk of onset or exacerbation of periodontal disease in the subject based on the stratification result.

[0085] The present disclosure also includes a method for assisting in determining (presenting) an oral hygiene product or an oral hygiene method suitable for a subject according to the presence or absence or degree of periodontal disease in the subject. In the present specification, this method may be referred to as "a method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure".

[0086] The method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure preferably uses the presence or absence of plaque adhesion in the subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample as indicators. The method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure may further use age as an indicator.

[0087] The method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure preferably includes stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria. The method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure may further include stratifying the subject based on age.

[0088] Regarding the presence or absence of plaque adhesion, the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and the method of stratifying the subject based on the presence or absence of plaque adhesion and the presence or absence of detection of periodontal pathogenic bacteria, etc., the description of the "stratification method of the present disclosure" described above can be incorporated by reference.

[0089] According to the method for assisting in determining an oral hygiene product or an oral hygiene method of the present disclosure, based on the stratification result, it is possible to assist in determining (presenting) an oral hygiene product or an oral hygiene method suitable for the subject.

[0090] The present disclosure can provide a program for realizing a method for assisting in determining the oral hygiene product or oral hygiene method of the present disclosure described above. That is, the program causes a computer to have a first function of obtaining the presence or absence of plaque adhesion of a subject and the presence or absence of detection of periodontal pathogenic bacteria in an oral sample, and based on the presence or absence of plaque adhesion obtained by the first function and the presence or absence of detection of periodontal pathogenic bacteria in the oral sample, a second function of stratifying the subject, and a third function of determining (presenting) an oral hygiene product or oral hygiene method suitable for the subject based on the stratification result.

[0091] In addition, in this specification, "comprising" includes "consisting essentially of" and "consisting of" (The term "comprising" includes "consisting essentially of” and "consisting of."). Further, the present disclosure includes all arbitrary combinations of the constituent elements described in this specification.

[0092] Moreover, the various characteristics (properties, structures, functions, etc.) described for each embodiment of the present disclosure above may be combined in any manner when specifying the subject matter encompassed by the present disclosure. That is, the present disclosure includes all subject matters consisting of any combination of the combinable characteristics described in this specification.

Example

[0093] The content of the present disclosure will be specifically described using the following test examples. However, the present disclosure is not limited to these in any way. In the following, unless otherwise specifically mentioned, the experiments are carried out under atmospheric pressure and normal temperature conditions.

[0094] For 449 subjects (297 males and 152 females (average age 45.5 years)), the following items were confirmed according to the described methods respectively.

[0095] Test Example 1 Presence or absence of plaque (measurement of PlI (Plaque Index)) The PlI was measured for six representative teeth per person (Figure 1). The representative teeth were the upper right 6, upper right 1, upper left 6, lower right 6, lower left 1, and lower left 6. However, when 6 was missing, the measurement was taken at 7. PlI is an index indicating the degree of dental plaque adhesion. The PlI was measured for six representative teeth according to the following criteria. The value at the most adherent site (maximum value) in one tooth was adopted. <Criteria> 0: No adhesion was observed 1: Dental plaque adhesion less than 1 / 3 of the tooth surface 2: Dental plaque adhesion from 1 / 3 to less than 2 / 3 of the tooth surface 3: Dental plaque adhesion of 2 / 3 or more of the tooth surface The average value was calculated from the PlI of six representative teeth. When the average value of PlI was 0, it was designated as "no plaque", and when the average value of PlI was greater than 0, it was designated as "plaque present".

[0096] Test Example 2 Analysis of salivary flora DNA was extracted from the saliva of the subjects, the 16S rRNA gene possessed by bacteria was amplified by PCR, and then bacterial flora analysis was performed using the next-generation sequencer Miseq (Illumina, Inc.). (The minimum value of the composition ratio of the detected periodontal pathogenic bacteria was about 0.001%. Since there were about 10 8 cfu / mL of bacteria in the saliva, the detection limit was estimated to be about 10 3 cfu / mL). From the results of the analysis, the groups were classified into those with or without the detection of Porphyromonas gingivalis (sometimes denoted as Pg) (groups below the detection limit value).

[0097] Test Example 3 Classification of periodontal diseases The CPI (Community Periodontal Index) was measured by a dental professional's examination. <Criteria> Code 0: Sound Code 1: Bleeding Code 2: Calculus deposition Code 3: Shallow periodontal pocket (PD = 4 - 5 mm) Code 4: Deep periodontal pocket (PD ≥ 6 mm) The codes of representative teeth were measured respectively, and the maximum code was taken as the representative value for each subject. By combining codes 1 and 2 as gingivitis, each subject was classified into the following 4 groups.

[0098]

Table 1

[0099] 1. Classification by the presence or absence of plaque The classification results of periodontal diseases in the group with plaque attachment and the group without plaque attachment are shown below.

[0100]

Table 2

[0101] In the group with plaque attachment, it was confirmed that the proportion of subjects with gingivitis or periodontitis was high. On the other hand, even in the group without plaque attachment, since about 24% of the subjects had periodontitis, it was found that the presence or absence and degree of periodontal disease could not be predicted only by the presence or absence of plaque attachment (there is a possibility of missing subjects at risk of periodontal disease).

[0102] 2. Classification by the presence or absence of plaque and salivary Pg After classifying into the group with plaque attachment and the group without plaque attachment, it was further classified into the group with salivary Pg detection and the group without salivary Pg detection. The classification results of periodontal diseases in each group are shown below.

[0103]

Table 3

[0104] Even in the group without plaque attachment, in the group with salivary Pg detection, it was confirmed that the proportion of subjects with periodontitis was higher compared to the group without salivary Pg detection. In addition, in the group with plaque attachment, among the group with detectable salivary Pg, the proportion of subjects with periodontitis (especially severe periodontitis) was higher compared to other groups, and in the group without detectable salivary Pg, the proportion of subjects with gingivitis was higher compared to other groups. Further, when compared with the case of classification only by the presence or absence of plaque ([Table 2]), it was confirmed that in the group with plaque attachment and detectable salivary Pg, the proportion of subjects with gingivitis decreased and the proportion of subjects with periodontitis increased. In addition, when there is plaque attachment, the proportion of subjects without periodontal disease is low, and the proportion of subjects with gingivitis and mild periodontitis is high. Therefore, it is considered that plaque attachment is involved in the onset (occurrence of inflammation) of periodontal disease. Also, when there is detectable salivary Pg, the proportion of subjects with gingivitis is low, and the proportion of subjects with mild periodontitis and severe periodontitis is high. Therefore, it is considered that the presence or absence of periodontal pathogens in oral samples is involved in the exacerbation of periodontal disease. From the above, it is suggested that by classifying in combination with the presence or absence of plaque attachment and salivary Pg, it is possible to predict the presence or absence or the degree of periodontal disease more accurately compared to the case of classification only by the presence or absence of plaque attachment. It is suggested that the combination of the presence or absence of plaque attachment and salivary Pg is an effective evaluation index for predicting the presence or absence or the degree of periodontal disease.

[0105] 3. Classification by the presence or absence of plaque, salivary Pg, and age After classification into the group with plaque attachment and the group without plaque attachment, it was further classified into the group with detectable salivary Pg and the group without detectable salivary Pg. In the group with detectable salivary Pg, it was further classified into the group of those 40 years old or older and the group of those under 40 years old. The classification results of periodontal disease in each group are shown below.

[0106]

Table 4

[0107] Even in the group without plaque attachment, salivary Pg was detected. In the group with salivary Pg detection and an age of 40 years or older, it was confirmed that the proportion of subjects with periodontitis was higher compared to the group with an age of less than 40 years. Furthermore, when compared with the classification combining plaque attachment and salivary Pg ([Table 3]), in the group without plaque attachment, with salivary Pg detection, and an age of less than 40 years, it was confirmed that the proportion of subjects with periodontitis decreased and the proportion of subjects without periodontal disease increased. Also, in the group with plaque attachment, in the group with salivary Pg detection and an age of 40 years or older, it was confirmed that the proportion of subjects with periodontitis (especially severe periodontitis) was higher compared to the group with an age of less than 40 years. From the above, it was suggested that by classifying in combination with the presence or absence of plaque attachment, salivary Pg, and age, it is possible to predict the presence or absence or degree of periodontal disease more accurately compared to classifying only by the presence or absence of plaque attachment or by combining plaque attachment and salivary Pg. It was suggested that the combination of the presence or absence of plaque attachment, salivary Pg, and age is a more effective evaluation index for determining the presence or absence or degree of periodontal disease.

[0108] 4. Classification by the Presence or Absence of Plaque, Salivary Pg, and Age After classifying into the group with plaque attachment and the group without plaque attachment, it was further classified into the group with salivary Pg detection and the group without salivary Pg detection. In the group with salivary Pg detection, it was further classified into the group with an age of 50 years or older and the group with an age of less than 50 years. The classification results of periodontal disease in each group are shown below.

[0109]

Table 5

[0110] In the group without plaque adhesion and with detection of salivary Pg, when classified by age of 50 years, the difference in the proportion of subjects with periodontitis between the group aged 50 years or older and the group under 50 years became smaller compared to when classified by age of 40 years ([Table 4]) (in the group under 50 years of age, the proportion of subjects with mild or severe periodontitis increased), which was confirmed. Also, in the group with plaque adhesion and with detection of salivary Pg, when classified by age of 50 years, the difference in the proportion of subjects with periodontitis between the group aged 50 years or older and the group under 50 years became smaller compared to when classified by age of 40 years ([Table 4]) (in the group under 50 years of age, the proportion of subjects with periodontitis increased), and it was confirmed that the proportion of subjects without periodontal disease decreased. In addition, when classified by age of 30 years, the number of subjects in whom salivary Pg was detected was small (about 20%), suggesting that it was not appropriate as a classification criterion for age. From this, it was suggested that when using age as an index, by setting the standard at 40 years, the presence or absence or degree of periodontal disease can be judged more accurately.

Claims

1. A method for stratifying subjects, comprising: classifying the subjects into a group with plaque attachment or a group without plaque attachment, and classifying the subjects into a group with detection of periodontal pathogens in an oral sample collected from the subjects or a group without such detection and suggesting that subjects with plaque attachment and detection of periodontal pathogens in the oral sample are highly likely to have severe periodontitis, suggesting that subjects with plaque attachment but without detection of periodontal pathogens in the oral sample are highly likely to have gingivitis, suggesting that subjects without plaque attachment but with detection of periodontal pathogens in the oral sample are highly likely to have periodontitis, and suggesting that subjects without plaque attachment and without detection of periodontal pathogens in the oral sample are highly likely not to have periodontal disease.

2. A method for stratifying subjects, comprising: classifying the subjects into a group with plaque attachment or a group without plaque attachment, classifying the subjects into a group with detection of periodontal pathogens in an oral sample collected from the subjects or a group without such detection, and comparing the age of the subjects with a reference value and classifying the subjects into a group above the reference value or a group below the reference value, suggesting that subjects with plaque attachment, detection of periodontal pathogens in the oral sample, and an age above the reference value are highly likely to have severe periodontitis, suggesting that subjects with plaque attachment, detection of periodontal pathogens in the oral sample, and an age below the reference value are highly likely to have gingivitis, suggesting that subjects without plaque attachment, with detection of periodontal pathogens in the oral sample, and an age above the reference value are highly likely to have periodontitis, and A method characterized in that a subject without plaque adhesion, with detection of periodontal pathogenic bacteria in the oral sample, and with an age less than the reference value is suggested to be likely not to have periodontal disease.

3. The method according to claim 2, wherein the reference value of age is 40 years old.

4. The method according to any one of claims 1 to 3, characterized in that it is used to determine an oral hygiene product or an oral hygiene method suitable for the subject.

5. A method for determining an oral hygiene product suitable for a subject, classifying the subject into a group with plaque adhesion or a group without plaque adhesion, and classifying the subject into a group with detection of periodontal pathogenic bacteria or a group without detection of periodontal pathogenic bacteria in an oral sample collected from the subject including, and further, for a subject with plaque adhesion and with detection of periodontal pathogenic bacteria in the oral sample, determining that an oral hygiene product for improving severe periodontitis is a suitable oral hygiene product, for a subject with plaque adhesion and without detection of periodontal pathogenic bacteria in the oral sample, determining that an oral hygiene product for improving gingivitis is a suitable oral hygiene product, for a subject without plaque adhesion and with detection of periodontal pathogenic bacteria in the oral sample, determining that an oral hygiene product for improving periodontitis is a suitable oral hygiene product, and for a subject without plaque adhesion and without detection of periodontal pathogenic bacteria in the oral sample, determining that an oral hygiene product for preventing periodontal disease is a suitable oral hygiene product, including, a method.

6. A method for determining an oral hygiene product suitable for a subject, classifying the subject into a group with plaque adhesion or a group without plaque adhesion, classifying the subject into a group with detection of periodontal pathogenic bacteria or a group without detection of periodontal pathogenic bacteria in an oral sample collected from the subject, and Comparing the age of the subject with a reference value and classifying the subject into a group of equal to or greater than the reference value or a group of less than the reference value, and further, For subjects with plaque adhesion, detection of periodontal pathogens in the oral sample, and an age equal to or greater than the reference value, it is determined that an oral hygiene product for improving severe periodontitis is a suitable oral hygiene product. For subjects with plaque adhesion, detection of periodontal pathogens in the oral sample, and an age less than the reference value, it is determined that an oral hygiene product for improving gingivitis is a suitable oral hygiene product. For subjects without plaque adhesion, detection of periodontal pathogens in the oral sample, and an age equal to or greater than the reference value, it is determined that an oral hygiene product for improving periodontitis is a suitable oral hygiene product, and For subjects without plaque adhesion, detection of periodontal pathogens in the oral sample, and an age less than the reference value, it is determined that an oral hygiene product for preventing the onset of periodontal disease is a suitable oral hygiene product. A method comprising the above.

7. The method according to claim 6, wherein the reference value of age is 40 years old.

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