Amplifying plate assembly and method for detecting its state

Abstract

To provide an amplification plate assembly which solves a problem that a sealing method of a nucleic acid amplification plate is complex.SOLUTION: Provided is an amplification plate assembly, including: an amplification plate body 10 provided with a plurality of accommodating grooves 11; a frame structure 20 covering on the amplification plate body and including a mounting opening 211; a sealing film disposed on the frame structure and sealing the mounting opening; and an insertion structure 30 disposed between the frame structure and the amplification plate body, wherein the insertion structure 30 includes an insertion portion 31 and a cooperating portion cooperating with the insertion portion, the cooperating portion includes a first cooperating portion and a second cooperating portion 33, when the insertion portion is cooperated with the first cooperating portion, there is a predetermined distance between the sealing film and the amplification plate body, and when the insertion portion is cooperated with the second cooperating portion, the sealing film is attached to the amplification plate body.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to the field of consumables for nucleic acid detection, and specifically to an amplification plate assembly and a method for detecting its state.

Background Art

[0002] An amplification plate assembly is used to amplify nucleic acid extracted from a sample for subsequent detection. Before performing nucleic acid amplification, it is necessary to transfer the nucleic acid extracted from the sample to the amplification plate assembly and seal the amplification plate assembly using a sealing film.

[0003] In related technologies, a nucleic acid amplification plate is designed to facilitate the film sealing operation of a sample for nucleic acid detection. The nucleic acid amplification plate in related technologies needs to be used in combination with a sealing film reel. Specifically, the sealing film reel is attached to a sealing film reel fixing structure. When it is necessary to seal the amplification plate body, the sealing film is pulled out from the sealing film reel, and the sealing film is heat-pressed onto the amplification plate body by a sealing machine for sealing. Finally, the excess sealing film is cut by a cutting mechanism to complete the sealing of the nucleic acid amplification plate.

[0004] The sealing method of the nucleic acid amplification plate in related technologies is complicated and requires combination with an additional sealing film reel fixing structure and cutting structure, occupying space.

Summary of the Invention

Problems to be Solved by the Invention

[0005] The main object of the present invention is to provide an amplification plate assembly that solves the problem that the sealing method of the nucleic acid amplification plate in related technologies is complicated.

Means for Solving the Problems

[0006] To achieve the above object, the present invention provides an amplification plate assembly, comprising: an amplification plate body provided with a plurality of accommodation grooves; a frame structure covering the amplification plate body and having mounting openings facing the groove openings of the plurality of accommodation grooves; a sealing film provided on the frame structure for closing the mounting openings; and an insertion structure provided between the frame structure and the amplification plate body, including an insertion portion and an engaging portion engaged with the insertion portion, wherein one of the insertion portion and the engaging portion is provided on the frame structure and the other is provided on the amplification plate body, the engaging portion includes a first engaging portion and a second engaging portion, when the insertion portion is inserted into and engaged with the first engaging portion, a predetermined distance is provided between the sealing film and the amplification plate body, and when the insertion portion is inserted into and engaged with the second engaging portion, the sealing film is provided in close contact with the upper surface of the amplification plate body.

[0007] Furthermore, the first engaging portion and the second engaging portion are provided at intervals on one side of the amplification plate body or the frame structure so that the switching of the insertion structure between the first engaging portion and the second engaging portion can be realized by translating the frame structure.

[0008] Furthermore, the insertion portion includes insertion protrusions provided on the frame structure, the first engaging portion includes a first insertion groove, the second engaging portion includes a second insertion groove, both the first insertion groove and the second insertion groove are provided on the amplification plate body, and the groove bottom wall of the second insertion groove is lower than that of the first insertion groove.

[0009] Furthermore, the first insertion groove and the second insertion groove are provided at intervals on one side of the amplification plate body, and the switching of the insertion protrusions between the first insertion groove and the second insertion groove is realized by translating the frame structure.

[0010] Furthermore, there are a plurality of insertion protrusions, a plurality of insertion protrusions are provided at intervals at the first end of the frame structure, a plurality of insertion protrusions are provided at intervals at the second end of the frame structure, there are a plurality of first insertion grooves provided in one-to-one correspondence with the plurality of insertion protrusions, and there are a plurality of second insertion grooves provided in one-to-one correspondence with the plurality of insertion protrusions.

[0011] Furthermore, the first insertion groove is provided on the side wall of the amplification plate body and penetrates the top wall of the amplification plate body, and the second insertion groove is provided on the side wall of the amplification plate body and penetrates the top wall of the amplification plate body.

[0012] Furthermore, the frame structure includes an upper frame and side frames provided in the circumferential direction of the upper frame. The side frames are located on the outer side in the circumferential direction of the side wall of the amplification plate body. The amplification plate assembly further includes a first insertion hole provided on the side wall of the amplification plate body and a second insertion hole provided on the side frame. When the insertion protrusion is inserted into and engaged with the first insertion groove, the first insertion hole and the second insertion hole are provided opposite to each other. When the insertion part is inserted into and engaged with the second insertion groove, the first insertion hole and the second insertion hole are provided opposite to each other.

[0013] Furthermore, when the insertion part is inserted into and engaged with the first insertion groove, the projection of the first insertion hole on the side frame is completely located within the second insertion hole. When the insertion part is inserted into and engaged with the second insertion groove, at least a part of the projection of the first insertion hole on the side frame is located within the second insertion hole.

[0014] Furthermore, the amplification plate body includes a first end and a second end provided opposite to each other in the length direction of the amplification plate body. The first insertion holes are plural. A plurality of first insertion holes are provided on the side wall of the first end of the amplification plate body, and a plurality of first insertion holes are provided on the side wall of the second end of the amplification plate body. The first insertion grooves are plural. A plurality of first insertion grooves are provided on the side wall of the first end of the amplification plate body, and a plurality of first insertion grooves are provided on the side wall of the second end of the amplification plate body. The second insertion grooves are plural. A plurality of second insertion grooves are provided on the side wall of the first end of the amplification plate body, and a plurality of second insertion grooves are provided on the side wall of the second end of the amplification plate body.

[0015] Furthermore, the amplification plate body further includes a plurality of connecting rings provided in a one-to-one correspondence with a plurality of accommodating grooves. Each connecting ring is provided at the groove opening of the accommodating groove and is provided concentrically with respect to the groove opening of the accommodating groove. The upper surface of the connecting ring forms the upper surface of the amplification plate body.

[0016] Furthermore, the plurality of receiving grooves are provided in an array. A first identifier is provided in the first first receiving groove of each row, and a second identifier is provided in the first receiving groove of each column.

[0017] The present invention is a method for detecting the state of an amplification plate assembly. The amplification plate assembly is the above-described amplification plate assembly. The amplification plate body of the amplification plate assembly has a first long edge. When the insertion part is inserted into and engaged with the second engaging part, the distance that the frame structure protrudes from the first long edge of the amplification plate body is set as L1. When the insertion part is inserted into and engaged with the first engaging part, the distance that the frame structure protrudes from the first long edge of the amplification plate body is set as L4. The method for detecting the state of the amplification plate assembly includes moving the amplification plate body so that the amplification plate body is close to the signal transmission line between the signal transmitter and the signal receiver and has a first predetermined distance H1 (H1 < L1 and H1 < L4) between the first long edge of the amplification plate body and the signal transmission line between the signal transmitter and the signal receiver, and determining whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver has received the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate body. If the signal receiver has not received the signal transmitted from the signal transmitter, it is determined that the frame structure covers the amplification plate body. Further provided is a method for detecting the state of an amplification plate assembly.

[0018] The present invention is a method for detecting the state of an amplification plate assembly, where the amplification plate assembly is the above-described amplification plate assembly. The amplification plate body of the amplification plate assembly has a first long edge and a second long edge. When the insertion part is inserted into and engaged with the second engagement part, the distance L1 is defined as the distance that the frame structure protrudes from the first long edge of the amplification plate body, and the distance L2 is defined as the distance that the frame structure protrudes from the second long edge of the amplification plate body. The method for detecting the state of the amplification plate assembly is as follows: Move the amplification plate body so that the first long edge of the amplification plate body is close to the signal transmission line between the signal transmitter and the signal receiver, and there is a second predetermined distance H2 (L2 < H2 < L1) between the first long edge of the amplification plate body and the signal transmission line between the signal transmitter and the signal receiver. Then, determine whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate body. If the signal receiver has not received the signal transmitted from the signal transmitter, it is determined that the insertion part is inserted into and engaged with the second engagement part. Further provided is a method for detecting the state of an amplification plate assembly including the above steps.

[0019] The present invention relates to a method for detecting the state of an amplification plate assembly. The amplification plate assembly is the above-described amplification plate assembly. The amplification plate body of the amplification plate assembly has a first long edge and a second long edge. When the insertion part is inserted into and engaged with the first engagement part, the distance L4 is defined as the distance that the frame structure protrudes from the first long edge of the amplification plate body, and the distance L3 is defined as the distance that the frame structure protrudes from the second long edge of the amplification plate body. The method for detecting the state of the amplification plate assembly is as follows: move the amplification plate body so that the second long edge of the amplification plate body is close to the signal transmission line between the signal transmitter and the signal receiver, and there is a third predetermined distance H3 (L4 < H3 < L3) between the second long edge of the amplification plate body and the signal transmission line between the signal transmitter and the signal receiver; determine whether the signal receiver receives the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate body. If the signal receiver does not receive the signal transmitted from the signal transmitter, it is determined that the insertion part is inserted into and engaged with the first engagement part. Further provided is a method for detecting the state of the amplification plate assembly including the above steps.

Effect of the Invention

[0020] According to the technical aspect of the present invention, the amplification plate assembly includes an amplification plate body and a frame structure. An attachment port is provided in the frame structure, and the attachment port is closed by a sealing film. The sealing film is provided opposite to a plurality of accommodation grooves. The sealing film can be attached to the amplification plate body by heat pressing of a heat press machine to close the plurality of accommodation grooves and prevent the detection sample in the accommodation grooves from overflowing. On the other hand, an insertion part is further provided in the frame structure, and a first engagement part and a second engagement part that respectively engage with the insertion part are provided on the amplification plate body. When transporting the amplification plate assembly, the insertion part can be inserted into the first engagement part. At this time, since there is a predetermined distance between the sealing film and the amplification plate body, scratches are not generated between the sealing film and the amplification plate body due to the shaking during transportation, and the probability of the sealing film being damaged is effectively reduced. When it is necessary to seal the accommodation grooves, the insertion part can be inserted into the second engagement part. At this time, the sealing film is provided in close contact with the upper surface of the amplification plate body, and the sealing film can be heated by a heat press machine and adhered to the amplification plate body to realize the sealing of the accommodation grooves. In the above structure, by engaging the insertion part with different engagement parts, the probability of the sealing film being damaged during the transportation of the amplification plate assembly can be effectively reduced, and the sealing method between the sealing film and the amplification plate body is simple and rapid. Film sealing can be realized only by a heat press machine, and there is no need to separately provide other auxiliary devices.

[0021] The drawings for the specification constituting a part of the present application are for further understanding of the present invention, and the schematic embodiments and their descriptions in the present invention interpret the present invention and do not unduly limit the present invention. In the drawings,

Brief Description of the Drawings

[0022]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Embodiments for Carrying Out the Invention

[0023] Explanation of Reference Signs 10... Amplification plate body, 11... Accommodation groove, 12... First end, 13... Second end, 14... Connecting ring, 15... First identifier, 16... Second identifier, 20... Frame structure, 21... Upper frame, 211... Mounting port, 22... Side frame, 30... Insertion structure, 31... Insertion part, 311... Insertion protrusion, 32... First engaging part, 321... First insertion groove, 33... Second engaging part, 331... Second insertion groove, 40... First insertion hole, 50... Second insertion hole.

[0024] Hereinafter, while referring to the drawings in the embodiments of the present invention, the technical aspects of the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. The following description of at least one exemplary embodiment is actually only an exemplification and does not limit the present invention and its application or use in any way. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without the need for creative labor are included within the protection scope of the present invention.

[0025] It should be noted that the terms used herein are only for explaining specific embodiments and are not intended to limit the exemplary embodiments of the present application. As used herein, unless otherwise clearly indicated in the context, even if the singular form is used, it shall be construed to include the plural form. Also, when terms such as "including" and / or "having" are used in this specification, it should be understood that they indicate the presence of features, things, operations, devices, assemblies, and / or combinations thereof.

[0026] Unless otherwise specified, the members and the relative arrangements, mathematical formulas, and numerical values described in these embodiments do not limit the scope of the present invention. At the same time, for the convenience of explanation, it should be understood that the dimensions of each part shown in the drawings are not drawn according to the actual proportional relationship. Although the techniques, methods, and devices known to those skilled in the art may not be discussed in detail, when appropriate, such techniques, methods, and devices should be regarded as part of the patented specification. In all the examples shown and discussed herein, any specific value is not limiting and should be construed merely as illustrative. Therefore, other examples of the exemplary embodiments can have different values. It should be noted that in the following drawings, similar reference signs and letters indicate similar items. Therefore, if an item is defined in one drawing, there is no need to discuss it further in subsequent drawings.

[0027] As shown in FIGS. 1 to 3, the amplification plate assembly of the present embodiment includes an amplification plate body 10, a frame structure 20, a sealing film, and an insertion structure 30. Here, a plurality of accommodation grooves 11 are provided in the amplification plate body 10. The frame structure 20 covers the amplification plate body 10, and the frame structure 20 has mounting openings 211 facing the openings of the plurality of accommodation grooves 11. The sealing film is provided on the frame structure 20 to close the mounting openings 211. The insertion structure 30 is provided between the frame structure 20 and the amplification plate body 10, and the insertion structure 30 includes an insertion portion 31 and an engaging portion that engages with the insertion portion 31. The insertion portion 31 is provided on the frame structure 20, and the engaging portion is provided on the amplification plate body 10. The engaging portion includes a first engaging portion and a second engaging portion 33. When the insertion portion 31 is inserted into and engaged with the first engaging portion, there is a predetermined distance between the sealing film and the amplification plate body 10. When the insertion portion 31 is inserted into and engaged with the second engaging portion 33, the sealing film is provided in close contact with the upper surface of the amplification plate body 10.

[0028] According to the amplification plate assembly according to this embodiment, the amplification plate assembly includes an amplification plate body 10 and a frame structure 20. An attachment port 211 is opened in the frame structure 20, and the attachment port 211 is closed by a sealing film. The sealing film is provided opposite to a plurality of accommodation grooves 11. The sealing film can be attached to the amplification plate body 10 by hot pressing of a hot press machine to close the plurality of accommodation grooves 11 and prevent the specimen sample in the accommodation grooves from overflowing. On the other hand, an insertion part 31 is further provided in the frame structure 20, and a first engagement part and a second engagement part 33 that respectively engage with the insertion part 31 are provided on the amplification plate body 10. When transporting the amplification plate assembly, the insertion part 31 can be inserted into and engaged with the first engagement part. At this time, since there is a predetermined distance between the sealing film and the amplification plate body 10, no scratches will occur between the sealing film and the amplification plate body 10 due to the shaking during transportation, effectively reducing the probability of the sealing film being damaged. When it is necessary to seal the accommodation grooves 11, the insertion part 31 can be inserted into and engaged with the second engagement part 33. At this time, the sealing film is provided in close contact with the upper surface of the amplification plate body 10, and the sealing film can be heated by a hot press machine and adhered to the amplification plate body 10 to achieve the sealing of the accommodation grooves 11. In the above structure, by engaging the insertion part 31 with different engagement parts, the probability of the sealing film being damaged during the transportation of the amplification plate assembly can be effectively reduced. At the same time, the sealing method between the sealing film and the amplification plate body 10 is simple and rapid, and film sealing can be realized only by a hot press machine, without the need to separately provide other auxiliary devices.

[0029] Of course, in other embodiments not shown, the insertion part may be provided on the amplification plate body, and the engagement part may be provided on the frame structure.

[0030] As shown in FIGS. 1 to 3, in the present embodiment, the insertion part 31 includes an insertion protrusion 311 provided on the frame structure 20, the first engaging part includes a first insertion groove 321, the second engaging part 33 includes a second insertion groove 331, the first insertion groove 321 and the second insertion groove 331 are both provided on the amplification plate body 10, and the groove bottom wall of the second insertion groove 331 is lower than the groove bottom wall of the first insertion groove 321. In the above structure, when the insertion protrusion 311 is inserted into the first insertion groove 321, there is a predetermined distance between the sealing film and the amplification plate body 10. When the insertion protrusion 311 is inserted into the second insertion groove 331, the sealing film is provided in close contact with the upper surface of the amplification plate body 10. At the same time, the insertion protrusion 311, the first insertion groove 321, and the second insertion groove 331 have a simple structure and are easy to process. The connection between the insertion protrusion 311 and the first insertion groove 321 and the second insertion groove 331 is simple, and the assembly can be completed quickly.

[0031] As shown in FIGS. 1 to 3, in the present embodiment, the first insertion groove 321 and the second insertion groove 331 are provided at intervals on one side of the amplification plate body 10 so that the insertion protrusion 311 can be switched between the first insertion groove 321 and the second insertion groove 331 by translating the frame structure 20. With the above structure, it is easy to switch the insertion protrusion 311 between the first insertion groove 321 and the second insertion groove 331. Specifically, in the present embodiment, the frame structure 20 realizes the switching of the insertion protrusion 311 between the first insertion groove 321 and the second insertion groove 331 by being gripped by the gripping device. When moving the insertion protrusion 311 from the first insertion groove 321 into the second insertion groove 331, the gripping device moves downward to grip the frame structure 20, horizontally moves the amplification plate body 10, and then the gripping device lowers the frame structure 20, whereby the switching of the insertion protrusion 311 from the first insertion groove 321 to the second insertion groove 331 can be realized.

[0032] As shown in FIGS. 1 to 3, in the present embodiment, there are a plurality of insertion protrusions 311. A plurality of insertion protrusions 311 are provided at intervals on the first end 12 of the frame structure 20, and a plurality of insertion protrusions 311 are provided at intervals on the second end 13 of the frame structure 20. There are a plurality of first insertion grooves 321, which are provided in a one-to-one correspondence with the plurality of insertion protrusions 311. There are a plurality of second insertion grooves 331, which are provided in a one-to-one correspondence with the plurality of insertion protrusions 311. In the above structure, a plurality of insertion protrusions 311 are provided at intervals on the first end 12 of the frame structure 20. Similarly, a plurality of insertion protrusions 311 are provided at intervals on the second end 13 of the frame structure 20. With such an installation mode, when the frame structure 20 covers the amplification plate body 10, it has a plurality of contact points, so that the frame structure 20 can stably cover the amplification plate body 10. Accordingly, in order to ensure that the insertion protrusions 311 can be inserted into and engaged with the first insertion grooves 321 and the second insertion grooves 331 respectively, the first insertion grooves 321 and the second insertion grooves 331 need to be provided corresponding to the insertion protrusions 311.

[0033] As shown in FIGS. 1 to 3, in the present embodiment, the first insertion groove 321 is provided on the side wall of the amplification plate body 10 and penetrates the top wall of the amplification plate body 10. The second insertion groove 331 is provided on the side wall of the amplification plate body 10 and penetrates the top wall of the amplification plate body 10. In the above structure, both the first insertion groove 321 and the second insertion groove 331 are provided on the side wall of the amplification plate body 10 and can penetrate the top wall. Therefore, the insertion protrusion 311 can be inserted into the first insertion groove 321 or the second insertion groove 331 from top to bottom, and the assembly of the frame structure 20 and the amplification plate body 10 can be smoothly carried out. At the same time, with such an installation mode, the space on the side wall of the amplification plate body 10 is effectively utilized, and in the amplification plate assembly, the first insertion groove 321 and the second insertion groove 331 are prevented from excessively occupying the space on the top wall of the amplification plate body 10.

[0034] As shown in FIGS. 1 to 3, in the present embodiment, the frame structure 20 includes an upper frame 21 and side frames 22 provided in the circumferential direction of the upper frame 21. The side frames 22 are located on the outer side in the circumferential direction of the side wall of the amplification plate body 10. The amplification plate assembly further includes a first insertion hole 40 provided in the side wall of the amplification plate body 10 and a second insertion hole 50 provided in the side frame 22. When the insertion protrusion 311 is inserted and engaged with the first insertion groove 321, the first insertion hole 40 and the second insertion hole 50 are provided opposite to each other. When the insertion part 31 is inserted and engaged with the second insertion groove 331, the first insertion hole 40 and the second insertion hole 50 are provided opposite to each other. In the above structure, the frame structure 20 can realize the movement of the amplification plate assembly by the gripping of the gripping device, and can realize the switching of the insertion protrusion 311 between the first insertion groove 321 and the second insertion groove 331. Specifically, when the amplification plate body 10 is arranged away from the frame structure 20, the clamping claws of the gripping device can be individually inserted into the first insertion hole 40 of the amplification plate body 10 to realize the individual clamping of the amplification plate body 10. It is also possible to individually insert into the second insertion hole 50 of the frame structure 20 to realize the individual clamping of the frame structure 20. When the frame structure 20 covers the amplification plate body 10, regardless of whether the insertion protrusion 311 of the frame structure 20 engages with the first insertion groove 321 or the second insertion groove 331, the clamping claws of the gripping device can be simultaneously inserted into the first insertion hole 40 and the second insertion hole 50 to realize the overall movement of the amplification plate assembly.

[0035] Note that the area of the first insertion hole 40 is smaller than the area of the second insertion hole 50. Since the frame structure 20 has two covering states, by increasing the area of the second insertion hole 50, when the insertion projection 311 of the frame structure 20 engages with the first insertion groove 321, the overlapping area of the first insertion hole 40 and the second insertion hole 50 becomes sufficiently large. Therefore, when the insertion projection 311 engages with the first insertion groove 321, the clamping claws can be simultaneously inserted into the first insertion hole 40 and the second insertion hole 50, making it easier to clamp the entire amplification plate assembly. Accordingly, when the insertion projection 311 of the frame structure 20 engages with the second insertion groove 331, the overlapping area of the second insertion hole 50 and the first insertion hole 40 is also increased, and the clamping claws can be simultaneously inserted into the first insertion hole 40 and the second insertion hole 50.

[0036] Specifically, as shown in FIGS. 1 to 3, when the insertion portion 31 is inserted into and engaged with the first insertion groove 321, the projection of the first insertion hole 40 onto the side frame 22 is completely located within the second insertion hole 50. When the insertion portion 31 is inserted into and engaged with the second insertion groove 331, at least a part of the projection of the first insertion hole 40 onto the side frame 22 is located within the second insertion hole 50. With the above structure, since the first insertion hole 40 has a sufficient area for the clamping claws of the gripping device to be inserted, the stability when the gripping device moves the entire amplification plate assembly can be enhanced. Preferably, in this embodiment, the projection of the second insertion hole 50 onto the side frame 22 completely covers the first insertion hole 40.

[0037] As shown in FIGS. 1 to 3, in the present embodiment, the amplification plate body 10 has a first end 12 and a second end 13 provided opposite to each other in the length direction of the amplification plate body 10. The first insertion holes 40 are plural, and a plurality of first insertion holes 40 are provided on the side wall of the first end 12 of the amplification plate body 10, and a plurality of first insertion holes 40 are provided on the side wall of the second end 13 of the amplification plate body 10. The first insertion grooves 321 are plural, and a plurality of first insertion grooves 321 are provided on the side wall of the first end 12 of the amplification plate body 10, and a plurality of first insertion grooves 321 are provided on the side wall of the second end 13 of the amplification plate body 10. The second insertion grooves 331 are plural, and a plurality of second insertion grooves 331 are provided on the side wall of the first end 12 of the amplification plate body 10, and a plurality of second insertion grooves 331 are provided on the side wall of the second end 13 of the amplification plate body 10. In the above structure, since the plurality of first insertion holes 40, the plurality of first insertion grooves 321, and the plurality of second insertion grooves 331 are provided at the first end 12 and the second end 13 in the length direction of the amplification plate body 10 respectively, when switching the insertion projection 311 between the first insertion groove 321 and the second insertion groove 331, the frame structure 20 can be grasped and lifted by the clamping claws and translated, and the switching of the insertion projection 311 between the first insertion groove 321 and the second insertion groove 331 can be realized.

[0038] In addition, in the present embodiment, the upper edge of the second insertion hole 50 is longer than the upper edge of the first insertion hole 40. Also, in the present embodiment, the clamping claws are composed of a plurality of clamping blocks, and the plurality of clamping blocks can be close to or separated from each other. When clamping the frame structure 20, the distance between the plurality of clamping blocks is increased to ensure that the clamping blocks are inserted only into the second insertion hole 50 and not into the first insertion hole 40, thereby realizing the clamping of the frame structure 20. When clamping the entire amplification plate assembly, the distance between the plurality of clamping blocks is decreased so that the clamping blocks can be inserted into both the first insertion hole 40 and the second insertion hole 50 simultaneously, thereby realizing the overall clamping of the frame structure 20.

[0039] The consumables for nucleic acid detection further include an extraction plate for nucleic acid detection. The extraction plate for nucleic acid detection is a 96-well plate with a larger volume than the amplification plate assembly. The length of the amplification plate assembly can be set to the same size as the width of the extraction plate for nucleic acid detection. Insertion holes into which the clamping claws of the gripping device are inserted are provided in the length direction of the amplification plate assembly, and the insertion holes of the extraction plate for nucleic acid detection are provided in the width direction of the extraction plate for nucleic acid detection. In this way, the extraction plate for nucleic acid detection and the amplification plate assembly can be gripped by the same gripping device, improving the versatility of the amplification plate assembly.

[0040] As shown in FIGS. 1 to 3, in this embodiment, the amplification plate body 10 further includes a plurality of connecting rings 14. The plurality of connecting rings 14 are provided in a one-to-one correspondence with the plurality of accommodating grooves 11. Each connecting ring 14 is provided at the groove opening of the accommodating groove 11 and is provided concentrically with respect to the groove opening of the accommodating groove 11. The upper surface of the connecting ring 14 forms the upper surface of the amplification plate body 10. In the above structure, the heat press machine can adhere the sealing film to the connecting ring 14 by heat pressing. The connecting ring 14 can enhance the adhesion effect between the amplification plate body 10 and the sealing film, ensuring the sealing effect of the accommodating groove 11 by the sealing film.

[0041] As shown in FIGS. 1 to 3, in this embodiment, the plurality of accommodating grooves 11 are provided in an array. A first label 15 is provided in the first accommodating groove 11 in each row, and a second label 16 is provided in the first accommodating groove 11 in each column. In the above structure, the first label 15 can be a character label, and the second label 16 can be a numerical label, making it easy for the operator to position any one of the plurality of accommodating grooves 11.

[0042] In this embodiment, when viewed in the direction from top to bottom, the amplification plate body 10 has a first rectangular structure having two long edges (the first long edge and the second long edge, respectively) provided opposite to each other, and two short edges (corresponding to the first end 12 and the second end 13 of the amplification plate body 10 described above, respectively) connected between both ends of the first long edge and both ends of the second long edge. Here, a plurality of first insertion grooves 321 and a plurality of second insertion grooves 331 corresponding one-to-one to the plurality of first insertion grooves 321 are provided at the first end 12 of the amplification plate body 10. As shown in FIG. 3, the adjacent first insertion groove 321 and second insertion groove 331 form a set of insertion groove structures. Among the set of insertion groove structures, the first insertion groove 321 is provided close to the second long edge, and the second insertion groove 331 among the set of insertion groove structures is provided close to the first long edge. That is, in FIG. 3, the long edge located in the upper right is the first long edge, and the long edge located in the lower left is the second long edge. In FIGS. 4 and 5, the long edge located on the right side is the first long edge, and the long edge located on the left side is the second long edge.

[0043] As shown in FIGS. 1 and 2, when viewed in the direction from top to bottom, the frame structure 20 has a second rectangular structure, and the length of the second rectangular structure is larger than the length of the first rectangular structure. Therefore, the frame structure 20 can cover the amplification plate body 10. Further, since the second rectangular structure is wider than the first rectangular structure and has a certain interval, the frame structure 20 can be moved relative to the amplification plate body 10 in the width direction. As a result, the insertion portion 31 can be switched between a position where it engages with the first engaging portion 32 and a position where it engages with the second engaging portion 33. FIG. 4 is a schematic side configuration diagram of the amplification plate assembly when the insertion portion 31 engages with the second engaging portion 33. The distance L1 by which the frame structure 20 protrudes from the first long edge of the amplification plate body 10 is larger than the distance L2 by which the frame structure 20 protrudes from the second long edge of the amplification plate body 10. FIG. 5 is a schematic side configuration diagram of the amplification plate assembly when the insertion portion 31 engages with the first engaging portion 32. The distance L3 by which the frame structure 20 protrudes from the second long edge of the amplification plate body 10 is larger than the distance L4 by which the frame structure 20 protrudes from the first long edge of the amplification plate body 10.

[0044] The present invention further provides a method for detecting the state of an amplification plate assembly. The amplification plate assembly is the above-described amplification plate assembly. The method for detecting the state of the amplification plate assembly includes moving the amplification plate body 10 so that the amplification plate body 10 is close to the line connecting the signal transmitter and the signal receiver, and has a first predetermined distance H1 between the first long edge of the amplification plate body 10 and the line connecting the signal transmitter and the signal receiver; and determining whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate body 10. If the signal receiver has not received the signal transmitted from the signal transmitter, it is determined that the frame structure covers the amplification plate body 10.

[0045] In the above aspect, a method for detecting the state of an amplification plate assembly is provided. Specifically, the amplification plate body 10 can be moved to a position close to the signal transmission line between the signal transmitter and the signal receiver, and a first predetermined distance H1 can be provided between the first long edge of the amplification plate body 10 and the signal transmission line between the signal transmitter and the signal receiver. Then, it is determined whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is found that since there is no object shielding the signal, the frame structure 20 does not cover the amplification plate body 10. Also, if the signal receiver has not received the signal transmitted from the signal transmitter, it is found that the signal is shielded by the frame structure 20, and at this time, it can be determined that the frame structure covers the amplification plate body 10. In the above aspect, the covering state of the amplification plate assembly can be determined through the signal transmitter and the signal receiver, facilitating the subsequent film sealing operation of the amplification plate assembly.

[0046] When the insertion part 31 engages with the second engaging part 33, let the distance that the frame structure 20 protrudes from the first long edge of the amplification plate body 10 be L1, and when the insertion part 31 engages with the first engaging part 32, let the distance that the frame structure 20 protrudes from the first long edge of the amplification plate body 10 be L4. As shown in FIGS. 4 and 5, L4 is smaller than L1.

[0047] In addition, the above-mentioned first predetermined distance H1 is smaller than the distance that the frame structure 20 protrudes from the first long edge of the amplification plate body 10. The distance that the frame structure 20 protrudes from the first long edge of the amplification plate body 10 mentioned here means the smaller one of L1 and L4, that is, the above distance means L4.

[0048] The above-mentioned signal transmission line refers to the transmission path from the transmission of a signal by a signal transmitter to the reception of the signal by a signal receiver, and may be a straight connection line formed between the signal transmitter and the signal receiver in a opposed type optocoupler, or in a reflective type optocoupler, the signal transmitter and the signal receiver are located at the same end, and the signal receiver receives the signal by receiving the reflected light.

[0049] The present invention further provides a method for detecting the state of an amplification plate assembly. The amplification plate assembly is the above-mentioned amplification plate assembly, and the method for detecting the state of the amplification plate assembly is to move the amplification plate body 10 so that the first long edge of the amplification plate body 10 is close to the signal transmission line between the signal transmitter and the signal receiver, and there is a second predetermined distance H2 between the first long edge of the amplification plate body 10 and the signal transmission line between the signal transmitter and the signal receiver, and to determine whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate body 10. If the signal receiver has not received the signal transmitted from the signal transmitter, it is determined that the insertion part 31 is inserted into and engaged with the second engaging part 33.

[0050] In the above aspect, not only can the coating state of the amplification plate assembly be determined through the signal transmitter and the signal receiver, but also it is possible to determine which engaging portion the insertion portion 31 of the frame structure is specifically engaged with. Specifically, when the insertion portion 31 is inserted into and engaged with the second engaging portion 33, the distance L1 by which the frame structure 20 protrudes from the first long edge of the amplification plate main body 10 is greater than the distance L2 by which the frame structure 20 protrudes from the second long edge of the amplification plate main body 10. A second predetermined distance H2 can be set so as to satisfy L2 < H2 < L1. When the signal transmitted from the transmitter is blocked by the frame structure, it is determined that the insertion portion 31 is inserted into and engaged with the second engaging portion 33, that is, it is found that the sealing film of the frame structure at that time is attached to the amplification plate main body 10.

[0051] The present invention further provides a method for detecting the state of an amplification plate assembly. The amplification plate assembly is the above-described amplification plate assembly. The method for detecting the state of the amplification plate assembly includes moving the amplification plate main body 10 such that the second long edge of the amplification plate main body 10 is close to the signal transmission line between the signal transmitter and the signal receiver (that is, the line connecting the signal transmitter and the signal receiver), and having a third predetermined distance H3 between the second long edge of the amplification plate main body 10 and the signal transmission line between the signal transmitter and the signal receiver, and determining whether the signal receiver has received the signal transmitted from the signal transmitter. If the signal receiver can receive the signal transmitted from the signal transmitter, it is determined that the frame structure does not cover the amplification plate main body 10. If the signal receiver has not received the signal transmitted from the signal transmitter, it is determined that the insertion portion 31 is inserted into and engaged with the first engaging portion 32.

[0052] In the above aspect, not only can the coating state of the amplification plate assembly be determined through the signal transmitter and the signal receiver, but it is also possible to determine which engaging portion the insertion portion 31 of the frame structure is specifically engaged with. Specifically, when the insertion portion 31 is inserted into and engaged with the first engaging portion 32, the distance L3 by which the frame structure 20 protrudes from the second long edge of the amplification plate body 10 is greater than the distance L4 by which the frame structure 20 protrudes from the first long edge of the amplification plate body 10. A third predetermined distance H3 can be set such that L4 < H3 < L3. When the signal transmitted from the transmitter is blocked by the frame structure, it is found that the insertion portion 31 is inserted into and engaged with the second engaging portion 33, that is, there is a predetermined distance between the sealing film of the frame structure and the amplification plate body 10 at that time.

[0053] In the above-described embodiment, the signal transmitted from the signal transmitter is a laser signal, and both the signal transmitter and the signal receiver are fixedly installed. By moving the amplification plate assembly, detection cooperation with the signal transmitter and the signal receiver is realized. In other embodiments, the signal transmitter and the signal receiver may be provided movably, the amplification plate assembly may be fixedly arranged, and detection cooperation may be realized by moving the signal transmitter and the signal receiver.

[0054] In the description of the present invention, the orientation or positional relationship indicated by orientation words such as "front, rear, top, bottom, left, right", "horizontal, vertical, perpendicular, horizontal", "top, bottom", etc. is usually based on the orientation or positional relationship shown in the drawings. This is merely for the convenience of explaining the present invention and simplifying the explanation. Unless otherwise stated, these orientation words do not indicate that the indicated device or element must have a specific orientation or be configured and operate in a specific orientation. It should be understood that they do not limit the protection scope of the present invention. Also, the orientation words "inside, outside" mean the inside and outside of the contour of each member itself.

[0055] For the sake of explanation, here, spatial relative terms such as "above...", "over...", "on the upper surface of...", "upper", etc. are used to describe the spatial positional relationship between a device or feature as illustrated and other devices or features. It should be understood that spatial relative terms are intended to include different orientations in uses or operations other than the illustrated orientation of the device. For example, when the device in the drawing is inverted, a device described as "above another device or structure" or "on another device or structure" will be described as "below another device or structure" or "under another device or structure" after inversion. For this reason, the exemplary term "above..." can include two orientations, "above..." and "below...". This device may be positioned in other different ways (rotated 90 degrees or in other orientations), and accordingly, the spatial relative descriptions used here are interpreted.

[0056] Note that the use of terms such as "first", "second", etc. to limit components is merely for the convenience of distinguishing the corresponding components. Without other explanations, the above terms have no special meaning and should not be understood as limiting the protection scope of the present invention.

[0057] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art can make various changes and modifications to the present invention. Any modifications, equivalent replacements, improvements, etc. within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An amplification plate body (10) provided with a plurality of accommodation grooves (11); A frame structure (20) covering the amplification plate body (10) and having mounting openings (211) facing the groove openings of the plurality of accommodation grooves (11); A sealing film provided on the frame structure (20) for closing the mounting openings (211); An insertion structure (30) provided between the frame structure (20) and the amplification plate body (10), including an insertion part (31) and an engagement part engaging with the insertion part (31). One of the insertion part (31) and the engagement part is provided on the frame structure (20), and the other is provided on the amplification plate body (10). The engagement part includes a first engagement part (32) and a second engagement part (33). When the insertion part (31) is inserted into and engaged with the first engagement part (32), there is a predetermined distance between the sealing film and the amplification plate body (10). When the insertion part (31) is inserted into and engaged with the second engagement part (33), the sealing film is provided in close contact with the upper surface of the amplification plate body (10). The insertion structure (30) is included; The first engagement part (32) and the second engagement part (33) are provided at intervals on one side of the amplification plate body (10) or the frame structure (20) so that the switching of the insertion structure (30) between the first engagement part (32) and the second engagement part (33) is realized by translating the frame structure (20). The amplification plate assembly is characterized by this.

2. The insertion part (31) includes an insertion protrusion (311) provided on the frame structure (20); The first engagement part (32) includes a first insertion groove (321), the second engagement part (33) includes a second insertion groove (331), and both the first insertion groove (321) and the second insertion groove (331) are provided on the amplification plate body (10); The groove bottom wall of the second insertion groove (331) is lower than the groove bottom wall of the first insertion groove (321). The amplification plate assembly according to Claim 1 is characterized by this.

3. There are a plurality of the insertion protrusions (311). A plurality of the insertion protrusions (311) are provided at intervals at the first end of the frame structure (20), and a plurality of the insertion protrusions (311) are provided at intervals at the second end of the frame structure (20); There are a plurality of the first insertion grooves (321), which are provided in a one-to-one correspondence with the plurality of the insertion protrusions (311); The second insertion grooves (331) are plural and are provided in a one-to-one correspondence with the plurality of insertion projections (311). The amplification plate assembly according to claim 2, characterized in that.

4. The first insertion groove (321) is provided on the side wall of the amplification plate body (10) and penetrates the top wall of the amplification plate body (10). The second insertion groove (331) is provided on the side wall of the amplification plate body (10) and penetrates the top wall of the amplification plate body (10). The amplification plate assembly according to claim 2, characterized in that.

5. The frame structure (20) includes an upper frame (21) and side frames (22) provided in the circumferential direction of the upper frame (21). The side frames (22) are located on the outer side in the circumferential direction of the side wall of the amplification plate body (10). The amplification plate assembly further includes a first insertion hole (40) provided on the side wall of the amplification plate body (10) and a second insertion hole (50) provided on the side frame (22). When the insertion projection (311) is inserted into and engaged with the first insertion groove (321), the first insertion hole (40) and the second insertion hole (50) are provided opposite to each other. When the insertion portion (31) is inserted into and engaged with the second insertion groove (331), the first insertion hole (40) and the second insertion hole (50) are provided opposite to each other. The amplification plate assembly according to claim 2, characterized in that.

6. When the insertion portion (31) is inserted into and engaged with the first insertion groove (321), the projection of the first insertion hole (40) onto the side frame (22) is completely located within the second insertion hole (50). When the insertion portion (31) is inserted into and engaged with the second insertion groove (331), at least a part of the projection of the first insertion hole (40) onto the side frame (22) is located within the second insertion hole (50). The amplification plate assembly according to claim 5, characterized in that.

7. The amplification plate body (10) includes a first end (12) and a second end (13) provided opposite to each other in the length direction of the amplification plate body (10). The first insertion holes (40) are plural. A plurality of the first insertion holes (40) are provided on the side wall of the first end (12) of the amplification plate body (10), and a plurality of the first insertion holes (40) are provided on the side wall of the second end (13) of the amplification plate body (10). The plurality of the first insertion grooves (321) are provided on the side walls of the first end (12) of the amplification plate body (10), and the plurality of the first insertion grooves (321) are provided on the side walls of the second end (13) of the amplification plate body (10). The amplification plate assembly according to claim 5, wherein the plurality of the second insertion grooves (331) are provided on the side walls of the first end (12) of the amplification plate body (10), and the plurality of the second insertion grooves (331) are provided on the side walls of the second end (13) of the amplification plate body (10).

8. The amplification plate body (10) further includes a plurality of connecting rings (14) provided in a one-to-one correspondence with the plurality of the accommodating grooves (11). Each of the connecting rings (14) is provided at the groove opening of the accommodating groove (11) and is provided concentrically with respect to the groove opening of the accommodating groove (11). The amplification plate assembly according to any one of claims 1 to 7, wherein the upper surface of the connecting ring (14) forms the upper surface of the amplification plate body (10).

9. The plurality of the accommodating grooves (11) are provided in an array. A first identifier (15) is provided in the first accommodating groove (11) in each row. The amplification plate assembly according to any one of claims 1 to 7, wherein a second identifier (16) is provided in the first accommodating groove (11) in each column.

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