Method for culturing Kappaphycus alvarezii and method for producing feed

Abstract

An object of the present invention is to provide a method for culturing Kappaphycus alvarezii in large quantities in the sea and a method for producing a feed containing Kappaphycus alvarezii. 【Solution means】The method for culturing Kappaphycus alvarezii according to the present invention is a method for culturing Kappaphycus alvarezii using a culture plate, wherein the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body. The method for culturing Kappaphycus alvarezii includes the following steps (1) to (3). Step (1): A step of culturing a sporophyte in which tetrasporangia are formed using a culture plate Step (2): A step of culturing young gametophytes using the culture plate on which the sporophyte in which tetrasporangia are formed in step (1) is cultured Step (3): A step of culturing and collecting gametophytes in the sea using the culture plate on which the young gametophytes are cultured in step (2)

Description

Technical Field

[0001] The present invention relates to a method for culturing Asparagopsis taxiformis and a method for producing feed.

Background Art

[0002] Conventionally, as one of the causes of global warming, the emission of methane derived from the belches of livestock such as cows has been a problem. In Japan, as one of the efforts until 2050 in the "Green Food System Strategy" determined by the Ministry of Agriculture, Forestry and Fisheries, the item of "utilization of methane-suppressing cows" is listed, and as part of it, the development of feed that suppresses methane derived from cow belches has been attracting attention.

[0003] As one of the substances that suppress the emission of methane derived from livestock belches, attention has been paid to incorporating the red alga Asparagopsis taxiformis (scientific name: Asparagops taxiformis). Since Asparagopsis taxiformis contains a large amount of bromoform, it is considered that by feeding livestock with feed containing Asparagopsis taxiformis, the synthesis of methane generated in the intestines of livestock can be suppressed by bromoform.

[0004] However, Asparagopsis taxiformis is very brittle, and when trying to collect it from the sea, the algal thallus collapses, so it is difficult to efficiently recover Asparagopsis taxiformis growing on rocky reefs from the sea. Therefore, in recent years, for example, a method of culturing Asparagopsis taxiformis collected in natural waters on land using an aquarium has been carried out (for example, the method described in Patent Document 1, etc.).

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0006] However, in the above-described method of culturing on land, for example, it is not easy to set conditions such as light, water temperature changes, waves, and nutrients to conditions suitable for culturing similar to the natural environment. Therefore, it is difficult to culture a large amount of Gelidium amansii. Therefore, there is a need for a method of culturing a large amount of Gelidium amansii by culturing it in the sea, which is the natural environment itself.

[0007] Therefore, the present invention has been made in view of the above circumstances, and an object thereof is to provide a method for culturing Gelidium amansii capable of culturing a large amount of Gelidium amansii in the sea, and a method for producing a feed containing Gelidium amansii.

Means for Solving the Problems

[0008] The method for culturing Gelidium amansii according to the present invention is a method for culturing Gelidium amansii using a culture plate, wherein the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body, and comprises the following steps (1) to (3), a method for culturing Gelidium amansii. Step (1): A step of culturing a sporophyte in which tetrasporangia are formed using a culture plate Step (2): A step of culturing young gametophytes using the culture plate on which the sporophyte in which tetrasporangia are formed in step (1) is cultured Step (3): A step of culturing and collecting gametophytes in the sea using the culture plate on which the young gametophytes are cultured in step (2)

[0009] According to such a configuration, the method for culturing Gelidium amansii can culture in the sea until the young gametophytes become gametophytes while preventing the young gametophytes from floating in the sea due to the influence of ocean currents, waves, etc. Therefore, the method for culturing Gelidium amansii can culture a large amount of Gelidium amansii in the sea.

[0010] The method for culturing Gelidium amansii according to the present invention, as one aspect, step (1) is Step (1-1): A step of placing a first culture plate in a first water tank; Step (1-2): A step of discharging carpospores from the carposporophyte into the first water tank by introducing a gametophyte having a carposporophyte into the first water tank, and Step (1-3): A step of culturing a sporophyte in which a tetrasporangium is formed by allowing the carpospores to settle between adjacent second protrusions of a second culture plate adjacent to the first culture plate and growing the sporophyte until the tetrasporangium is formed; comprising Step (2) is Step (2-1): A step of recovering the first culture plate to which the sporophyte in which the tetrasporangium is formed is attached from the first water tank and placing the first culture plate in a second water tank, and Step (2-2): A step of culturing the young gametophyte by allowing the tetraspores discharged from the tetrasporangium to settle between adjacent second protrusions of a second culture plate placed in the second water tank, thereby forming a young gametophyte and fixing the stolon of the young gametophyte and the rhizoid filaments extending from the stolon to the second protrusions; comprising Step (3) is Step (3-1): A step of culturing the gametophyte by recovering the second culture plate to which the young gametophyte is attached from the second water tank and immersing it in the sea, and Step (3-2): A step of recovering the cultured gametophyte together with the second culture plate from the sea. comprising.

[0011] According to such a configuration, the method for culturing Kappaphycus alvarezii can efficiently settle tetraspores between adjacent second protrusions on the second culture plate by fixing the tetraspores discharged from the sporophyte in which the tetrasporangium is formed and fixed to the first culture plate in the second water tank, so that young gametophytes can be efficiently cultured.

[0012] In the method for culturing Kappaphycus alvarezii according to the present invention, steps (1) to (3) may be performed at a water temperature of 20°C or higher and 35°C or lower.

[0013] According to such a configuration, the method for culturing the keyhole laver can culture the keyhole laver in an environment close to its habitat environment, so that the culturing of the keyhole laver can be further promoted.

[0014] In the method for culturing the keyhole laver according to the present invention, the gametophyte having a carpogonium in step (1-2) may be derived from the collected gametophyte.

[0015] According to such a configuration, the method for culturing the keyhole laver can efficiently culture the keyhole laver.

[0016] In the method for culturing the keyhole laver according to the present invention, in a plurality of second protrusions, the distance between adjacent second protrusions may be 0.1 mm or more and 5.0 mm or less.

[0017] According to such a configuration, the method for culturing the keyhole laver can easily culture the keyhole laver because the temporary root filaments extending from the stolons of the keyhole laver can be easily fixed to the second protrusions.

[0018] In the method for culturing the keyhole laver according to the present invention, the shape of the second protrusion may be cylindrical, and its diameter may be 1.0 mm or more and 5.0 mm or less.

[0019] According to such a configuration, the method for culturing the keyhole laver can easily culture the keyhole laver because the temporary root filaments extending from the stolons of the keyhole laver can be easily fixed to the second protrusions.

[0020] The method for manufacturing a feed according to the present invention is a method for manufacturing a feed for livestock, and includes the following steps (1') to (3'). Step (1'): A step of culturing the keyhole laver using the above method for culturing the keyhole laver Step (2'): A step of drying the keyhole laver cultured in step (1') Step (3'): A step of mixing the keyhole laver dried in step (2') as a raw material for the feed

[0021] By including step (1'), the method for manufacturing the feed can efficiently obtain a large amount of Kagimochi-nori, so that the feed containing Kagimochi-nori can be efficiently and mass-produced.

Effects of the Invention

[0022] According to the present invention, it is possible to provide a method for culturing Kagimochi-nori capable of culturing a large amount of Kagimochi-nori in the sea, and a method for manufacturing a feed containing Kagimochi-nori.

Brief Description of the Drawings

[0023]

Figure 1

Figure 2

Figure 3

Figure 4

Modes for Carrying Out the Invention

[0024] Hereinafter, embodiments of the present invention will be described, but the present invention is not limited to the following embodiments.

[0025] (Method for Culturing Kagimochi-nori) Hereinafter, the method for culturing Kagimochi-nori according to the embodiment of the present invention will be described with reference to the drawings. In the following drawings, the same or corresponding parts are denoted by the same reference numerals, and the description thereof will not be repeated.

[0026] The method for culturing Kagimochi-nori according to the present embodiment is A method for culturing Kappaphycus using a culture plate 2, comprising: The culture plate 2 has a plate body 21 and a plurality of second protrusions 23 protruding from one surface S side of the plate body 21. It includes the following steps (1) to (3). Step (1): A step of culturing a sporophyte on which tetrasporangia are formed using the culture plate 2 Step (2): A step of culturing young gametophytes using the culture plate 2 on which the sporophyte on which tetrasporangia are formed in step (1) is cultured. Step (3): A step of culturing and recovering gametophytes in the sea using the culture plate 2 on which the young gametophytes are cultured in step (2).

[0027] Steps (1) to (3) are preferably carried out at a water temperature of 20°C or higher and 35°C or lower from the viewpoint of promoting the culture of Kappaphycus by culturing Kappaphycus in an environment close to its habitat environment.

[0028] [Culture plate] FIG. 1 is a perspective view of the culture plate 2 used in the method for culturing Kappaphycus according to this embodiment. The culture plate 2 has a plate body 21, a plurality of first protrusions 22, and a plurality of second protrusions 23.

[0029] The plurality of first protrusions 22 protrude from one surface S side of the plate body 21 and are configured to be able to fix the seed thread B of Kappaphycus shown in FIG. 4 described later.

[0030] The shape of the first protrusion 22 is cylindrical from the viewpoint of facilitating the winding of the seed thread B of Kappaphycus around the first protrusion. The diameter of the first protrusion 22 is preferably 5 mm or more and 15 mm or less, and more preferably 7 mm or more and 13 mm or less. Note that the shape, diameter, etc. of the first protrusion 22 are not limited to this as long as the seed thread B of Kappaphycus can be fixed.

[0031] The height of the first protrusion 22 is preferably 10 mm or more and 20 mm or less, more preferably 12 mm or more and 18 mm or less, from the viewpoint of facilitating the winding of the seed thread B of the sea tangle around the first protrusion 22 while preventing the tip of the gametophyte A from colliding with the first protrusion 22 and collapsing.

[0032] A plurality of the first protrusions 22 are formed in sets of ten each on both of a pair of portions (hereinafter, the sea tangle placement portions S1, S1) that are symmetric with respect to a line L connecting the centers of the opposing long sides among the four sides that are the peripheral edges of the plate body 21 when viewed from the one surface S side of the plate body 21. That is, a total of 20 are formed on the entire plate body 21. Note that the number of the first protrusions 22 is not limited to this as long as the seed thread B of the sea tangle can be fixed.

[0033] In the pair of sea tangle placement portions S1, S1, the ten first protrusions 22 are each arranged in three rows parallel to the long side of the plate body 21. Among them, the two outer rows are each composed of four first protrusions 22, and the central row is composed of two first protrusions 22. Note that the arrangement, intervals, etc. of the first protrusions 22 are not particularly limited.

[0034] A plurality of second protrusions 23 (corresponding to the protrusion described in the claims) project from the one surface S side of the plate body 21 and are configured such that the temporary root threads A1 extending from the stolons of the gametophyte A shown in FIG. 4 described later can be fixed thereto.

[0035] The interval between adjacent second protrusions 23, 23 is preferably 0.1 mm or more and 5.0 mm or less, more preferably 0.5 mm or more and 2.0 mm or less, from the viewpoint of facilitating the fixing of the temporary root threads A1 extending from the stolons of the gametophyte A to the second protrusions 23.

[0036] The shape of the second protrusion 23 is cylindrical from the viewpoint of facilitating the fixation of the adventitious root filaments A1 extending from the stolons of the gametophyte A to the second protrusion 23. Further, the diameter of the second protrusion 23 is preferably 1.0 mm or more and 5.0 mm or less, and more preferably 1.2 mm or more and 3.0 mm or less. Note that the shape, diameter, etc. of the second protrusion 23 are not limited to this as long as the adventitious root filaments A1 extending from the stolons of the gametophyte A can be fixed thereto. The method for culturing Kappaphycus according to the present embodiment, as one aspect, has a cylindrical shape of the second protrusion 23 and a diameter thereof of 1.0 mm or more and 5.0 mm or less.

[0037] The height of the second protrusion 23 is not particularly limited as long as the adventitious root filaments A1 extending from the stolons of the gametophyte A can be fixed thereto, and may be, for example, 1.0 mm or more and 5.0 mm or less, or may be 2.0 mm or more and 4.0 mm or less.

[0038] The same number of a plurality of second protrusions 23 are formed in both of the pair of Kappaphycus placement portions S1, S1. Further, the number of the plurality of second protrusions 23 is larger than the number of the plurality of first protrusions 22. Note that the number of the plurality of second protrusions 23 is not particularly limited as long as carpospores, sporophytes on which tetrasporangia are formed, tetraspores, young gametophytes, and gametophytes can be fixed thereto.

[0039] The plurality of second protrusions 23 are symmetrically arranged across the line L in the pair of Kappaphycus placement portions S1, S1. Further, the plurality of second protrusions 23 are arranged around the plurality of first protrusions 22.

[0040] As shown in Fig. 1, on one surface S of the plate body 21 of the culture plate 2 (i.e., the surface from which the first protrusions 22 and the second protrusions 23 protrude), a plurality of convex portions 24 are formed between the plurality of first protrusions 22 and the plurality of second protrusions 23. The shape, size, number, etc. of the convex shape constituting the convex portion 24 are not particularly limited, but the height of the convex portion 24 is preferably configured to be lower than the height of the second protrusion 23, for example, 5.0 mm or less. By forming the plurality of convex portions 24, the culture plate 2 can prevent shellfish or the like that inhibit the growth of Sargassum horneri from landing on one surface of the plate body 21, and can fix the rhizoid filaments A1 extending from the stolons of the gametophyte A to the convex portions constituting the plurality of convex portions 24.

[0041] As shown in Fig. 1, a hole 25 is formed in the central portion of one surface S of the plate body 21 of the culture plate 2, that is, at a position corresponding to the position between the pair of Sargassum horneri placement portions S1, S1. As will be described later, when the culture plate 2 is fixed to the concrete block 3, the bolt 27 is inserted into the hole 25 so that the culture plate 2 can be fixed to the concrete block 3.

[0042] As shown in Fig. 1, on the opposing short sides of the four sides that form the periphery of the culture plate 2, a pair of cuts 26, 26 are provided inward from the edge. The pair of cuts 26, 26 are formed in parallel with a gap at the central portion of one short side. Note that the position where the pair of cuts 26, 26 are formed is not particularly limited to the pair of opposing short sides, and may be formed on any of the four sides that form the periphery of the culture plate 2. By providing the pair of cuts 26, 26, the culture plate 2 can hook and fix the end of the seed thread B of Sargassum horneri to the pair of cuts 26, 26.

[0043] The material of the culture plate 2 is not particularly limited as long as carpospores, sporophytes on which tetrasporangia are formed, and tetraspores can adhere, and examples include synthetic resins and biodegradable resins.

[0044] Examples of the synthetic resin include polyethylene, polypropylene, polyvinyl chloride, polystyrene, syndiotactic polystyrene, ABS resin, AES resin, polyamide, polyacetal, polyester, polycarbonate, modified polyphenylene ether, polysulfone, polyarylate, polyetherimide, polyamideimide, polyphenylene sulfide, liquid crystal polyester, PEEK, PEN, paraffin wax, montan wax, carnauba wax, fatty acid ester, glycerite, modified wax, silane-modified polyolefin polymer, and the like.

[0045] Examples of the biodegradable resin include polyvinyl alcohol, polyglycolic acid, polybutylene succinate, polybutylene succinate-co-adipate, polybutylene adipate terephthalate, polyethylene terephthalate succinate, polylactic acid, polyhydroxyalkanoate, 3-hydroxybutyric acid-3-hydroxyhexane copolymer polyester, biopolybutylene succinate, polybutylene adipate terephthalate·polylactic acid compound, starch polyester resin, cellulose acetate, and the like.

[0046] The synthetic resin and the biodegradable resin may contain an inorganic filler. Examples of the inorganic filler include silica powder, calcium carbonate powder, limestone powder, shirasu powder, bone ash powder, diatomaceous earth powder, clay powder, and shell powders such as oyster, scallop, abalone, ark shell, clam, mussel, and clam, and coral powder. By including the inorganic filler, the strength of the culture plate 2 is improved and the seaweed is likely to grow.

[0047] As shown in FIG. 1, the shape of the culture plate 2 in plan view is rectangular. The shape of the culture plate in plan view is not particularly limited and may be, for example, trapezoidal, parallelogram-shaped, rhombus-shaped, or the like, other than rectangular.

[0048] [Step (1)] In step (1), using the culture plate 2, a sporophyte in which tetrasporangia are formed is cultured.

[0049] Specifically, step (1) is, as one aspect, Step (1-1): A step of placing the first culture plate 2 in the first water tank; Step (1-2): A step of putting a gametophyte having a carposporophyte into the first water tank, thereby discharging carpospores from the carposporophyte into the first water tank, and Step (1-3): A step of culturing a sporophyte in which tetrasporangia are formed by implanting carpospores between adjacent second protrusions 23, 23 of the first culture plate 2 and growing the sporophyte until a sporophyte is formed from the implanted carpospores and tetrasporangia are formed. includes.

[0050] <Step (1-1)> In step (1-1), the first culture plate 2 is placed in the first water tank.

[0051] The first culture plate 2 is the same as the above-described culture plate 2.

[0052] As an aspect of placing the first culture plate 2, for example, an aspect of suspending the first culture plate 2 from above the first water tank, an aspect of arranging the first culture plate 2 on the bottom surface of the first water tank, an aspect of arranging the first culture plate 2 on the side surface of the first water tank, etc. can be mentioned. Among these, from the viewpoint of facilitating the implantation of carpospores in step (1-3) described later, the aspect of placing the first culture plate 2 is preferably an aspect of arranging the first culture plate 2 on the bottom surface of the first water tank.

[0053] Note that the number of the first culture plates 2 installed in the first water tank is not particularly limited, and may be one or two or more.

[0054] In addition, when two or more first culture plates 2 are installed, from the viewpoint of efficiently attaching carpospores to the first culture plate 2, it is preferable that the first culture plates 2 be arranged without gaps.

[0055] <Step (1-2)> In step (1-2), by introducing a gametophyte having a carposporophyte into the first water tank, carpospores are released from the carposporophyte into the first water tank.

[0056] The gametophyte having a carposporophyte is preferably derived from the recovered gametophyte from the viewpoint of efficiently culturing Eucheuma striatum.

[0057] Examples of the recovered gametophyte include a gametophyte recovered from the habitat area of Eucheuma striatum, a gametophyte that has grown in a water tank and is recovered from the water tank, and the like. Among these, from the viewpoint of releasing more carpospores by using a gametophyte that has grown sufficiently in the natural environment, the recovered gametophyte is preferably a gametophyte recovered from the habitat area of Eucheuma striatum.

[0058] Examples of the mode of introducing the gametophyte having a carposporophyte include a method of introducing water containing the gametophyte having a carposporophyte into the first water tank, a method of installing a culture plate 2 to which the gametophyte having a carposporophyte is attached in the first water tank, and the like.

[0059] Note that the culture plate 2 to which the gametophyte having a carposporophyte is attached may be formed, for example, by a method of naturally implanting a gametophyte having a carposporophyte floating in the sea on one surface of the culture plate 2; a method of artificially implanting the collected gametophyte having a carposporophyte on one surface S of the culture plate 2, and the like.

[0060] <Step (1-3)> In step (1-3), by implanting carpospores between adjacent second protrusions 23, 23 of the first culture plate 2, a sporophyte is formed from the implanted carpospores, and the sporophyte is grown until a tetrasporangium is formed, thereby culturing the sporophyte in which the tetrasporangium is formed.

[0061] [Step (2)] In step (2), using the culture plate 2 on which the sporophyte in which the tetrasporangium was formed in step (1) was cultured, a young gametophyte is cultured.

[0062] Specifically, step (2) is, as one aspect, Step (2-1): recovering the first culture plate 2 to which the sporophyte in which the tetrasporangium was formed is attached from the first water tank and placing the first culture plate 2 in the second water tank, and Step (2-2): By implanting the tetraspores released from the tetrasporangium between adjacent second protrusions 23, 23 of the second culture plate 2 placed in the second water tank, a young gametophyte is formed from the tetraspores, and the stolon of the young gametophyte and the rhizoid filaments extending from the stolon are fixed to the second protrusion 23, thereby culturing the young gametophyte. includes.

[0063] <Step (2-1)> In step (2-1), the first culture plate 2 to which the sporophyte in which the tetrasporangium was formed is attached is recovered from the first water tank and the first culture plate 2 is placed in the second water tank.

[0064] Examples of the mode of installing the first culture plate 2 to which the sporophyte with tetrasporangia formed is attached include, for example, a mode of suspending the first culture plate 2 from above the second water tank, a mode of disposing the first culture plate 2 on the bottom surface of the second water tank, a mode of disposing the first culture plate 2 on the side surface of the second water tank, and the like. Among these, from the viewpoint of facilitating the efficient diffusion of the tetraspores described later into the second water tank, the mode of installing the first culture plate 2 is preferably a mode of suspending the first culture plate 2 from above the second water tank or a mode of disposing the first culture plate 2 on the side surface of the second water tank, and more preferably a mode of suspending the first culture plate 2 from above the second water tank.

[0065] Note that the number of the first culture plates 2 to which the sporophyte with tetrasporangia formed is attached and installed in the second water tank is not particularly limited, and it may be one or two or more.

[0066] <Step (2-2)> In step (2-2), by implanting the tetraspores released from the tetrasporangia between the adjacent second protrusions 23, 23 of the second culture plate 2 installed in the second water tank, young gametophytes are formed from the tetraspores, and the stolons of the young gametophytes and the rhizoids extending from the stolons are fixed to the second protrusions 23, thereby culturing the young gametophytes.

[0067] The second culture plate 2 is the same as the above-described culture plate 2.

[0068] Examples of the mode of installing the second culture plate 2 include, for example, a mode of suspending the second culture plate 2 from above the second water tank, a mode of disposing the second culture plate 2 on the bottom surface of the second water tank, a mode of disposing the second culture plate 2 on the side surface of the second water tank, and the like. Among these, from the viewpoint of facilitating the implantation of the tetraspores, the mode of installing the second culture plate 2 is preferably a mode of disposing the second culture plate 2 on the bottom surface of the second water tank.

[0069] Note that the number of the second culture plates 2 installed in the second water tank is not particularly limited, and may be one or two or more.

[0070] Further, when two or more second culture plates 2 are installed, from the viewpoint of efficiently attaching tetraspores to the second culture plates 2, it is preferable that the second culture plates 2 are arranged without gaps.

[0071] [Step (3)] In step (3), using the culture plate 2 on which the young gametophytes were cultured in step (2), the gametophytes are cultured and collected in the sea.

[0072] Specifically, step (3) is, as one aspect, Step (3-1): A step of culturing the gametophytes by recovering the second culture plate 2 to which the young gametophytes are attached from the second water tank and submerging it in the sea, and Step (3-2): A step of recovering the cultured gametophytes together with the second culture plate 2 from the sea, is included.

[0073] <Step (3-1)> In step (3-1), the second culture plate 2 to which the young gametophytes are attached is recovered from the second water tank and submerged in the sea to culture the gametophyte A.

[0074] Step (3-1) is, as one aspect, as shown in FIG. 2, culturing the gametophyte A by submerging the second culture plate 2 to which the young gametophytes are attached in the sea. FIG. 2 is a diagram showing the state of submerging the second culture plate 2 to which the young gametophytes are attached in the sea in one aspect of step (3-1). As shown in FIG. 2, the second culture plate 2 to which the young gametophytes are attached (hereinafter, also simply referred to as the culture plate 2) is attached to at least a part of the surface T of the concrete block 3.

[0075] As shown in FIG. 2, the culture plate 2 is disposed across the concrete block 3 at the center of the surface T of the concrete block 3. Note that the position where the culture plate 2 is disposed can be appropriately changed according to the size, shape, etc. of the culture plate 2.

[0076] The culture plate 2 is fixed by inserting bolts 27 into holes 25 (shown in FIG. 1) and fitting the bolts 27 into holes (not shown) provided on the surface of the concrete block 3.

[0077] A plurality of convex portions 31 are formed on the surface T of the concrete block 3 where the culture plate 2 is not attached.

[0078] As shown in FIG. 2, the shape of the convex portion 31 is rock-like. Note that the shape of the convex portion 31 is not particularly limited.

[0079] The height of the convex portion 31 is not particularly limited, and for example, it may be 5.0 mm or more and 30.0 mm or less.

[0080] The interval between adjacent convex portions 31, 31 is not particularly limited, and for example, it may be 5.0 mm or more and 40.0 mm or less.

[0081] Since a plurality of convex portions 31 are formed on the surface T of the concrete block 3 where the culture plate 2 is not attached, keyaki moss can be naturally implanted on the plurality of convex portions 31. Note that the arrangement of the plurality of convex portions 31 is not particularly limited, and for example, it may be irregular.

[0082] In another aspect, in step (3-1), as shown in FIG. 3, gametophyte A is cultured by submerging the second culture plate 2 to which young gametophytes are attached in the sea. FIG. 3 is a diagram showing the state of submerging the second culture plate 2 to which young gametophytes are attached in the sea in one aspect of step (3-1). As shown in FIG. 3, in addition to the above-mentioned concrete block 3, the culture plate 2 further includes a seaweed bed growth reef 4, and a plurality of pedestals 5 having the culture plate 2 are arranged on the upper surface of the seaweed bed growth reef 4.

[0083] As shown in FIG. 3, the upper surface of the pedestal 5 has the culture plate 2 attached to the concrete block 3. In FIG. 3, the culture plate 2 is attached to the concrete block 3, but the culture plate 2 may not be attached to the concrete block 3.

[0084] The height of the pedestal 5 is not particularly limited, and for example, it may be 0.3 m or more and 0.4 m or less.

[0085] The area of the upper surface of the pedestal 5 is not particularly limited as long as it can accommodate at least one concrete block 3. For example, it may be 2 0.2 m 2 or more and 0.3 m

[0086] The shape of the pedestal 5 is not particularly limited, and for example, it may be square, rectangular, etc. in top view.

[0087] The number of pedestals 5 arranged on the upper surface of the seaweed bed growth reef 4 can be appropriately changed according to the area of the upper surface of the seaweed bed growth reef 4 on which a plurality of pedestals 5 are arranged, which will be described later.

[0088] The height of the seaweed bed growth reef 4 is not particularly limited as long as it can be installed in the sea. For example, it may be 0.8 m or more and 1.0 m or less.

[0089] The area of the upper surface of the seaweed bed growth reef 4 is not particularly limited as long as a plurality of pedestals 5 equipped with the culture plates 2 can be arranged. For example, it may be 6.0 m 2 or more and 8.0 m 2 or less.

[0090] As shown in FIG. 3, the shape of the seaweed bed growth reef 4 is substantially hexagonal columnar. Note that the shape of the seaweed bed growth reef 4 is not particularly limited and can be appropriately changed according to, for example, the location of immersion, the environment such as ocean currents, etc.

[0091] As shown in FIG. 3, a cubic fence 6 is provided on the seaweed bed growth reef 4 so as to cover the pedestals 5 equipped with a plurality of the culture plates 2. By providing the fence 6 on the seaweed bed growth reef 4, the Kappaphycus can be protected from damage by fish or the like. Note that the shape of the fence 6 is not particularly limited as long as it can cover the pedestals 5 equipped with a plurality of the culture plates 2. Note that the fence 6 may not be provided on the seaweed bed growth reef 4.

[0092] Further, as shown in FIG. 3, a hanging portion 7 configured to be suspendable using a crane or the like is provided on the upper surface of the seaweed bed growth reef 4. By providing the hanging portion 7 on the seaweed bed growth reef 4, the entire culture plate 2 can be lifted from the sea, so that a large amount of Kappaphycus can be recovered without breaking the thalli of the Kappaphycus. In FIG. 3, the hanging portion 7 is provided on the upper surface where a plurality of pedestals 5 equipped with the culture plates 2 are arranged. However, as long as it is possible to recover without breaking the thalli of the Kappaphycus, the location of its arrangement is not particularly limited. Also, the shape of the hanging portion 7 is reverse U-shaped with respect to the upper surface of the seaweed bed growth reef 4, but its shape is not particularly limited.

[0093] Note that, as another aspect of step (3-1), although not shown in the drawings, one or more culture plates 2 may be attached to a floating member such as a raft with a rope or the like and suspended in the sea for immersion to culture the gametophytes.

[0094] The submerged location of the culture plate 2 is preferably a location at a water depth of 0.1 m or more and 15.0 m or less, more preferably a location at a water depth of 0.3 m or more and 10.0 m or less, from the viewpoint of culturing gametophytes under appropriate culture conditions.

[0095] In step (3-2), the cultured gametophyte A is recovered from the sea together with the second culture plate 2.

[0096] Examples of the method for recovering the cultured gametophyte A from the sea together with the second culture plate 2 include a method of recovering by diving and a method of lifting using a crane or the like. In the step (3-1), when the method of submerging the culture plate 2 is a method of attaching one or more culture plates 2 to a floating member such as a raft with a string or the like and suspending it in the sea for submerging, the method for recovering the cultured gametophyte A from the sea together with the culture plate 2 can adopt, in addition to the above recovery method, a method of winding up the rope to which the culture plate 2 is attached for recovery.

[0097] The method for culturing Kappaphycus according to the present embodiment, as a preferred embodiment, Step (1) is Step (1-1): A step of installing the first culture plate 2 in the first water tank, Step (1-2): A step of putting gametophytes having carposporophytes into the first water tank, whereby carpospores are released into the first water tank from the carposporophytes, and Step (1-3): By implanting carpospores between adjacent second protrusions 23, 23 of the first culture plate 2, a sporophyte is formed from the implanted carpospores, and the sporophyte is grown until a tetrasporangium is formed, thereby culturing the sporophyte in which the tetrasporangium is formed. including Step (2) is Step (2-1): A step of recovering the first culture plate 2 to which the sporophyte in which the tetrasporangium is formed is attached from the first water tank and installing the first culture plate 2 in the second water tank, and Step (2-2): By placing the tetraspores released from the tetrasporangium between the adjacent second protrusions 23, 23 of the second culture plate 2 installed in the second water tank, young gametophytes are formed from the tetraspores, and the stolons of the young gametophytes and the rhizoid filaments extending from the stolons are fixed to the second protrusion 2 to culture the young gametophytes. including Step (3) is Step (3-1): Recovering the second culture plate 2 to which the young gametophyte is attached from the second water tank and immersing it in the sea to culture the gametophyte A, and Step (3-2): Recovering the cultured gametophyte A together with the second culture plate 2 from the sea. including.

[0098] Note that, as shown in FIG. 4, the method for culturing Kagi-kennori according to the present embodiment may be performed by previously fixing the seed thread B of Kagi-kennori to the first protrusion 22 of the first culture plate 2 and / or the second culture plate 2. FIG. 4 is a diagram for explaining a method for culturing Kagi-kennori using the culture plate 2 to which the seed thread B of Kagi-kennori is fixed.

[0099] In this specification, the seed thread means a thread to which a gametophyte having a carpogonium, a carpospore, a sporophyte on which a tetrasporangium is formed, a tetraspore, a young gametophyte, or a gametophyte A is attached.

[0100] The seed thread B of Kagi-kennori is not particularly limited as long as it can attach carpogonia, sporophytes on which tetrasporangia are formed, and tetraspores and has flexibility and strength that can be fixed to the culture plate 2. For example, twisted threads such as ropes and octopus threads, wires, tapes, etc. can be mentioned.

[0101] The outer diameter of the seed thread B of Kagi-kennori is not particularly limited, and may be, for example, 1.0 mm or more and 40.0 mm or less.

[0102] The method for fixing the seed thread B of Kagi-kennori is, more specifically, as shown in Fig. 4. First, one end of the seed thread B of Kagi-kennori is sandwiched from one side S of the culture plate 2 toward the other side S' through one of the pair of cuts 26, 26 of the culture plate 2, and attached to the culture plate 2 such that one end of the seed thread B of Kagi-kennori is positioned on the other side S' of the culture plate 2. Then, with the seed thread B of Kagi-kennori having one end fixed, on one side S of the culture plate 2, it is hooked along the circumferential surface of each first protrusion 22 and locked while changing the direction, and by stretching it between each pair of first protrusions 22, the seed thread B of Kagi-kennori is fixed to the first protrusions 22 of the culture plate 2.

[0103] Also, as shown in Fig. 4, the method for fixing the seed thread B of Kagi-kennori is, after looping the seed thread B of Kagi-kennori around the first protrusions 22, further sandwiching the other end of the seed thread B of Kagi-kennori from one side S of the culture plate 2 toward the other side S' through the cut 26 of the pair of cuts 26, 26 of the culture plate 2 where one end of the seed thread B of Kagi-kennori has not been sandwiched, in the same manner as one end, and fixing the other end of the seed thread B of Kagi-kennori to the culture plate 2 as well. That is, by fixing both ends of the seed thread B of Kagi-kennori to the pair of cuts 26, 26 of the culture plate 2, the seed thread B of Kagi-kennori can be reliably attached to the culture plate 2.

[0104] The distance between the seed thread B of Kagi-kennori and one side S of the culture plate 2 is not particularly limited as long as the seed thread B of Kagi-kennori is in a moderately tense state and the adventitious root threads extending from the stolons of the gametophyte A can be fixed to the second protrusions 23. For example, it may be 30 mm or more and 50 mm or less.

[0105] By performing the above steps (1 - 3) using the first culture plate 2 to which the seed thread B of Kagi-kennori is fixed, the carpospores can settle not only between adjacent second protrusions 23, 23 of the first culture plate 2 but also on the seed thread B of Kagi-kennori.

[0106] Also, by performing the above step (2-2) using the second culture plate 2 on which the seed thread B of Sargassum horneri is fixed, the tetraspores can settle not only between adjacent second protrusions 23, 23 of the first culture plate 2 but also on the seed thread B of the Sargassum horneri.

[0107] The method for culturing Sargassum horneri according to the present embodiment is a method for culturing Sargassum horneri using a culture plate 2, wherein the culture plate 2 has a plate main body 21 and a plurality of second protrusions 23 protruding from one surface S side of the plate main body 21, and includes the following steps (1) to (3). Step (1): A step of culturing a sporophyte in which tetrasporangia are formed using the culture plate 2 Step (2): A step of culturing a young gametophyte using the culture plate 2 in which the sporophyte in which tetrasporangia are formed in step (1) is cultured Step (3): A step of culturing and collecting gametophyte A in the sea using the culture plate 2 in which the young gametophyte is cultured in step (2)

[0108] With such a configuration, the method for culturing Sargassum horneri can culture the young gametophyte in the sea until it becomes gametophyte A while preventing the young gametophyte from floating in the sea due to the influence of ocean currents, waves, etc. because the young gametophyte is fixed to the culture plate 2. Therefore, the method for culturing Sargassum horneri can culture a large amount of Sargassum horneri in the sea.

[0109] The method for culturing Sargassum horneri according to the present embodiment, as one aspect, step (1) is Step (1-1): A step of installing the first culture plate 2 in the first water tank, Step (1-2): A step of discharging carpospores into the first water tank by introducing a gametophyte having a carposporophyte into the first water tank, and Step (1 - 3): By implanting carpospores between adjacent second protrusions 23, 23 on the first culture plate, a sporophyte is formed from the implanted carpospores, and the sporophyte is grown until a tetrasporangium is formed, thereby culturing the sporophyte on which the tetrasporangium is formed. including Step (2) Step (2 - 1): Recovering the first culture plate 2 to which the sporophyte on which the tetrasporangium is formed is attached from the first water tank, and placing the first culture plate 2 in the second water tank, and Step (2 - 2): By implanting the tetraspores released from the tetrasporangium between adjacent second protrusions 23, 23 on the second culture plate 2 placed in the second water tank, a young gametophyte is formed from the tetraspores, and the stolon of the young gametophyte and the rhizoid filaments extending from the stolon are fixed to the second protrusion 23, thereby culturing the young gametophyte. including Step (3) Step (3 - 1): Recovering the second culture plate 2 to which the young gametophyte is attached from the second water tank and submerging it in the sea to culture gametophyte A, and Step (3 - 2): Recovering the cultured gametophyte A together with the second culture plate 2 from the sea. including.

[0110] With such a configuration, the method for culturing Kappaphycus striatum efficiently implants tetraspores between adjacent second protrusions 23, 23 on the second culture plate 2 by implanting the tetraspores released from the sporophyte on which the tetrasporangium is formed and fixed to the first culture plate 2 in the second water tank, so that young gametophytes can be efficiently cultured.

[0111] In the method for culturing Kappaphycus striatum according to this embodiment, steps (1) to (3) are performed at a water temperature of 20°C or higher and 35°C or lower, so that Kappaphycus striatum can be cultured in an environment close to its natural habitat, thus promoting the culture of Kappaphycus striatum more effectively.

[0112] In the method for culturing Kappaphycus alvarezii according to this embodiment, since the gametophyte having a carposporophyte in step (1-2) is derived from the collected gametophyte, Kappaphycus alvarezii can be efficiently cultured.

[0113] In the method for culturing Kappaphycus alvarezii according to this embodiment, in a plurality of second protrusions 23, since the distance between adjacent second protrusions 23, 23 is 0.1 mm or more and 5.0 mm or less, the temporary root filaments extending from the stolons of Kappaphycus alvarezii can be easily fixed to the second protrusions 23, so that Kappaphycus alvarezii can be easily cultured.

[0114] In the method for culturing Kappaphycus alvarezii according to this embodiment, since the shape of the second protrusion is cylindrical and its diameter is 1.0 mm or more and 5.0 mm or less, the temporary root filaments extending from the stolons of Kappaphycus alvarezii can be easily fixed to the second protrusions 23, so that Kappaphycus alvarezii can be easily cultured.

[0115] Note that the method for culturing Kappaphycus alvarezii according to the present invention can be variously modified without being limited to the above embodiment.

[0116] In the method for culturing Kappaphycus alvarezii according to the above embodiment, the use of the first culture plate 2 and the second culture plate 2 has been described. However, the method for culturing Kappaphycus alvarezii according to the present invention is not limited thereto, and for example, it may be performed using only one culture plate 2.

[0117] Also, in the method for culturing Kappaphycus alvarezii according to the above embodiment, the use of the first water tank and the second water tank has been described. However, the method for culturing Kappaphycus alvarezii according to the present invention is not limited thereto, and for example, it may be performed using only one water tank.

[0118] Also, in the method for culturing Kappaphycus alvarezii according to the above embodiment, the fact that the culture plate 2 has the first protrusion 22 has been described. However, the method for culturing Kappaphycus alvarezii according to the present invention is not limited thereto, and for example, the culture plate 2 may not have the first protrusion 22.

[0119] (Method for manufacturing feed) The method for manufacturing feed according to this embodiment is a method for manufacturing feed for livestock, and includes the following steps (1') to (3'). Step (1'): A step of culturing Kagimochi using the method for culturing Kagimochi Step (2'): A step of drying the Kagimochi cultured in step (1') Step (3'): A step of blending the dried Kagimochi in step (2') as a raw material for feed

[0120] [Step (1')] In step (1'), Kagimochi is cultured using the method for culturing Kagimochi.

[0121] The method for culturing Kagimochi is the same as the method for culturing Kagimochi according to the above embodiment.

[0122] [Step (2')] Step (2'): The Kagimochi cultured in step (1') is dried.

[0123] The method for drying Kagimochi is not particularly limited, and examples include a method of natural drying and a method of drying at a high temperature.

[0124] The drying temperature of Kagimochi is not particularly limited, and for example, it may be 10°C or higher and 90°C or lower, or it may be 20°C or higher and 80°C or lower.

[0125] [Step (3')] In step (3'), the Kagimochi dried in step (2') is blended as a raw material for feed.

[0126] The blending amount of Sargassum horneri is not particularly limited as long as it can suppress methane derived from livestock belching. For example, it may be 0.1% by mass or more and 50.0% by mass or less, 0.5% by mass or more and 30.0% by mass or less, or 1.0% by mass or more and 10.0% by mass or less with respect to the whole feed. Note that the blending amount of Sargassum horneri can be appropriately changed according to the type, size, gender, etc. of livestock.

[0127] In addition, the method for producing the feed according to the present embodiment may include a step of pulverizing dried Sargassum horneri before step (3') as necessary. By including this step, the method for producing the feed according to the present embodiment can uniformly blend Sargassum horneri in step (3').

[0128] Further, the method for producing the feed according to the present embodiment may include a molding step for molding the feed blended with Sargassum horneri into pellets or the like after step (3') as necessary. By including this step, the method for producing the feed according to the present embodiment can produce feed in a size that is easy for livestock to eat.

[0129] The method for producing the feed according to the present embodiment is a method for producing livestock feed. By including the above steps (1') to (3'), a large amount of Sargassum horneri can be efficiently obtained, so that the feed blended with Sargassum horneri can be efficiently and mass-produced.

[0130] Note that the method for culturing Sargassum horneri and the method for producing the feed according to the present invention are not limited to the above embodiments, and various modifications are possible without departing from the gist of the present invention.

[0131] The present invention includes the following aspects. [1] A method for culturing Sargassum horneri using a culture plate, wherein the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body, and a method for culturing Sargassum horneri comprising the following steps (1) to (3). Step (1): culturing the sporophyte on which tetrasporangia have formed using a culture plate Step (2): culturing young gametophytes using the culture plate on which the sporophyte on which tetrasporangia have formed in Step (1) has been cultured Step (3): culturing and recovering gametophytes in the sea using the culture plate on which young gametophytes have been cultured in Step (2) [2] Step (1) is Step (1-1): placing a first culture plate in a first water tank Step (1-2): introducing gametophytes having carposporophytes into the first water tank, thereby releasing carpospores from the carposporophytes into the first water tank, and Step (1-3): allowing the carpospores to settle between adjacent second protrusions of the first culture plate, growing the sporophyte formed from the settled carpospores until tetrasporangia are formed, thereby culturing the sporophyte on which tetrasporangia have formed including Step (2) is Step (2-1): recovering the first culture plate to which the sporophyte on which tetrasporangia have formed is attached from the first water tank and placing the first culture plate in a second water tank, and Step (2-2): allowing the tetraspores released from the tetrasporangia to settle between adjacent second protrusions of a second culture plate placed in the second water tank, thereby forming young gametophytes from the tetraspores and fixing the stolons of the young gametophytes and the rhizoid filaments extending from the stolons to the second protrusions, thereby culturing the young gametophytes including Step (3) is Step (3-1): recovering the second culture plate to which young gametophytes are attached from the second water tank and submerging it in the sea, thereby culturing the gametophytes, and Step (3-2): recovering the cultured gametophytes together with the second culture plate from the sea including the method for culturing Kappaphycus alvarezii according to [1]. [3] The method for culturing Kappaphycus alvarezii according to [1] or [2], wherein Steps (1) to (3) are performed at a water temperature of 20°C or higher and 35°C or lower [4] The gametophyte having a carpophyte in the project (1-2) is derived from the collected gametophyte, and the method for culturing Kappaphycus alvarezii according to [2]. [5] In a plurality of second protrusions, the distance between adjacent second protrusions is 0.1 mm or more and 5.0 mm or less, and the method for culturing Kappaphycus alvarezii according to any one of [1] to [4]. [6] The shape of the second protrusion is columnar, and its diameter is 1.0 mm or more and 5.0 mm or less, and the method for culturing Kappaphycus alvarezii according to any one of [1] to [5]. [7] A method for producing livestock feed, comprising the following steps (1') to (3'), and a method for producing feed. Step (1'): A step of culturing Kappaphycus alvarezii using the method for culturing Kappaphycus alvarezii according to any one of [1] to [6]. Step (2'): A step of drying the Kappaphycus alvarezii cultured in step (1'). Step (3'): A step of blending the Kappaphycus alvarezii dried in step (2') as a raw material for feed.

Explanation of symbols

[0132] 2 Culture plate 21 Plate body 22 First protrusion 23 Second protrusion 24 Convex part 25 Hole 26 Notch 27 Bolt 3 Concrete block 31 Convex part 4 Seaweed bed growth reef 5 Pedestal 6 Fence 7 Hanging part A Gametophyte A1 Temporary root filaments extending from the stolon of the gametophyte B Seed thread of Kappaphycus alvarezii L A line connecting the centers of the opposite long sides among the four sides that are the periphery of the plate body S One surface of the plate body S1 Placement part of Kappaphycus alvarezii S’ The other surface of the plate body Surface of the T-shaped concrete block

Claims

1. A method for culturing Kappaphycus using a culture plate, comprising: the culture plate having a plate body and a plurality of protrusions protruding from one surface side of the plate body; A method for culturing Kappaphycus, comprising the following steps (1) to (3). Step (1): Placing the culture plate in a water tank and culturing the sporophyte with tetrasporangia using the culture plate. Step (2): Using the culture plate on which the sporophyte with tetrasporangia formed in Step (1) was cultured, culturing young gametophytes in a water tank. Step (3): Using the culture plate on which the young gametophytes were cultured in Step (2), culturing and recovering the gametophytes in the sea.

2. Step (1) includes: Step (1-1): Placing a first culture plate in a first water tank. Step (1-2): By introducing gametophytes with carposporophytes into the first water tank, carpospores are released from the carposporophytes into the first water tank, and Step (1-3): By implanting carpospores between adjacent protrusions of the first culture plate, sporophytes are formed from the implanted carpospores, and the sporophytes are grown until tetrasporangia are formed, thereby culturing the sporophyte with tetrasporangia. including Step (2) includes: Step (2-1): Recovering the first culture plate to which the sporophyte with tetrasporangia is attached from the first water tank and placing the first culture plate in a second water tank, and Step (2-2): By implanting the tetraspores released from the tetrasporangia between adjacent protrusions of a second culture plate placed in the second water tank, young gametophytes are formed from the tetraspores, and the stolons of the young gametophytes and the rhizoids extending from the stolons are fixed to the protrusions, thereby culturing the young gametophytes. including Step (3) includes: Step (3-1): Recovering the second culture plate to which the young gametophytes are attached from the second water tank and immersing it in the sea to culture the gametophytes, and Step (3-2): Recovering the cultured gametophytes together with the second culture plate from the sea. The method for culturing Kappaphycus according to Claim 1, including.

3. The method for culturing Kappaphycus according to Claim 1, wherein steps (1) to (3) are performed at a water temperature of 20°C or higher and 35°C or lower.

4. The method for culturing Kappaphycus according to Claim 2, wherein the gametophytes with carposporophytes in Step (1-2) are derived from the recovered gametophytes.

5. The method for culturing Kagimori according to claim 1, wherein, in a plurality of protrusions, the distance between adjacent protrusions is 0.1 mm or more and 5.0 mm or less.

6. The method for culturing Kagimori according to claim 1, wherein the protrusions are columnar in shape and the diameter thereof is 1.0 mm or more and 5.0 mm or less.

7. A method for producing livestock feed, comprising the following steps (1') to (3'), the method for producing feed. Step (1'): A step of culturing Kagimori using the method for culturing Kagimori according to any one of claims 1 to 6 Step (2'): A step of drying the Kagimori cultured in step (1') Step (3'): A step of blending the Kagimori dried in step (2') as a raw material for feed

Images