External preparation for skin

Abstract

To provide an external preparation for skin, which exerts fibroblast-activating effects and collagen synthesis-promoting effects, obtained by finding out and blending a combination of ingredients that exhibits excellent preventive and ameliorating effects on skin aging, maintains or improves the skin in a youthful and healthy state, causes less irritation to the skin, and has excellent usability.SOLUTION: Provide are a fibroblast activator and a collagen synthesis promoter, both comprising niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an external preparation for skin that exhibits higher effectiveness by combining a plurality of components.

Background Art

[0002] Conventionally, the use of various compounds has been proposed as components that can be formulated in external preparations for skin, and external preparations for skin (such as external pharmaceuticals, quasi-drugs, and cosmetics) containing these compounds have been put on the market. For example, antioxidants such as vitamin C, vitamin E, and superoxide dismutase (hereinafter abbreviated as SOD); anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; cell-activating components such as α-hydroxycarboxylic acid, placenta extract, and γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, and hyaluronic acid; and humectants such as urea have been proposed. On the other hand, in consideration of safety, external preparations for skin containing various plant-derived components have been proposed. However, these compounds and plant-derived components alone are not sufficient in terms of effectiveness, and a combination of components that exhibits high effectiveness is required.

Disclosure of the Invention

Problems to be Solved by the Invention

[0003] In view of the problems of the above prior art, the present inventors have intensively studied combinations of highly effective compounds and plant-derived components as active ingredients of external preparations for skin. As a result, it has been found that a combination of niacinamide (alias: nicotinamide), a hydrolyzate of rice bran extract, and bamboo shoot extract exhibits high effectiveness as a component of an external preparation for skin. Conventionally, the effectiveness of niacinamide, the hydrolyzate of rice bran extract, and bamboo shoot extract alone is known from Patent Documents 1 to 5, but it has not been known that a synergistic effect is exhibited by combining them.

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Patent Document 5

Means for Solving the Problem

[0004] The present invention is a topical skin preparation containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients. Further, the present invention is a fibroblast activator containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients. Further, the present invention is a collagen synthesis promoter containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients.

Advantages of the Invention

[0005] The present invention exhibits a synergistic fibroblast activation effect and collagen synthesis promoting effect by combining niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract. Therefore, by formulating niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients, a highly effective topical skin preparation can be provided.

Brief Description of the Drawings

[0006]

Figure 1

Figure 2

Embodiments for Carrying Out the Invention

[0007] The rice bran used in the present invention is not particularly limited, and it is possible to use brown rice, germinated brown rice, polished rice, processed rice, pigmented rice (black rice, purple rice, red rice, etc.) or white bran and / or red bran contained in brown rice. As the type of rice, any of japonica rice, glutinous rice, etc. may be used. Examples of processed rice include anti-allergy rice, low-protein rice (e.g., low-glutelin rice), fortified rice (e.g., γ-aminobutyric acid rice), and the like.

[0008] In the present invention, first, an extract is prepared using the above-mentioned rice bran. The preparation of the extract is carried out by contacting the rice bran, which has been washed with water, dried, and finely cut or pulverized as necessary, with an extraction solvent by a conventional method such as an immersion method, a countercurrent extraction method, a steam distillation method, a subcritical extraction method, or a supercritical extraction method. Further, as will be described later, the extract is subjected to a hydrolysis treatment.

[0009] Examples of the extraction solvent include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination of two or more.

[0010] Among these extraction solvents, from the viewpoint of wide applicability to external skin preparations (cosmetics or quasi-drugs), the use of water or a mixed solvent of water and lower alcohols or water and polyhydric alcohols is preferred in the present invention.

[0011] In the present invention, when preparing an extract solution of rice bran, it is preferable to adjust the pH using an alkaline adjusting agent or an acidic adjusting agent as appropriate. Examples of the alkaline adjusting agent include sodium salts such as sodium hydroxide and sodium carbonate, and potassium salts such as potassium hydroxide. Among them, sodium hydroxide and sodium carbonate are preferable. Examples of the acidic adjusting agent include inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid, or organic acids such as acetic acid, lactic acid, malic acid, and citric acid.

[0012] For the extract obtained by the above treatment, a hydrolysis treatment is performed before extraction, after extraction, or in parallel with extraction. Examples of the hydrolysis treatment include methods using an acid, an alkali, or an enzyme, and the hydrolysis treatment using an enzyme is preferable.

[0013] Examples of the enzyme used for the hydrolysis treatment include proteolytic enzymes, amylase, cellulase, and lipase. These enzymes may be used alone or in combination of a plurality of enzymes according to the plant species.

[0014] Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase, bromelain, and complex proteolytic enzymes derived from microorganisms [e.g., Neurase (manufactured by Amano Enzyme Inc.)]. When using proteolytic enzymes, any of the above-mentioned enzymes can be used alone or in combination. Examples of amylolytic enzymes include α-amylase, β-amylase, glucoamylase, β-galactosidase, etc. When using amylolytic enzymes, any of the above-mentioned enzymes can be used alone or in combination. Examples of cellulolytic enzymes include cellulase, hemicellulase, pectinase, etc. When using cellulolytic enzymes, any of the above-mentioned enzymes can be used alone or in combination. Examples of lipolytic enzymes include lipase. When performing hydrolysis treatment with enzymes, one or more enzymes selected from the above enzyme groups can be used in combination.

[0015] The hydrolysis treatment using enzymes is carried out by adding one or more of the above enzymes to the above-mentioned extract solution of rice bran and causing an enzyme reaction under conditions near the optimum pH and optimum temperature of the enzyme used. When using a combination of two or more enzymes, depending on the characteristics of the enzymes used, the two or more enzymes may act simultaneously, or the reaction conditions may be changed or not changed, and they may act sequentially. The amount of enzyme used is preferably in the range of 0.001 to 50 parts by weight per 100 parts by weight of the solid content of the rice bran extract solution for one type of enzyme. The time of enzyme treatment varies depending on the type of enzyme used, etc., but generally ranges from 0.5 to 24 hours.

[0016] After the hydrolysis treatment using the above enzymes, the enzyme treatment solution is deactivated using an appropriate method such as heating the enzyme treatment solution to 80°C or higher to obtain an enzyme-treated decomposition product solution.

[0017] Next, the "bamboo shoots" used in the present invention will be described. "Bamboo shoots" are the young shoots of bamboo in the subfamily Bambusoideae of the family Poaceae. As the part of bamboo shoots used in the present invention, it may be the edible part, the non-edible skin part, or bamboo water.

[0018] Examples of the bamboo in the subfamily Bambusoideae of the family Poaceae used in the present invention include Phyllostachys bambusoides, Phyllostachys pubescens, Phyllostachys nigra, Phyllostachys aurea, Phyllostachys heterocycla, Bambusa multiplex, Semiarundinaria fastuosa, Sasa kurilensis, Sinobambusa tootsik, Chimonobambusa quadrangularis, Chimonobambusa marmorea, Pseudosasa japonica, and Pleioblastus simonii. However, the present invention is not limited thereto.

[0019] In the present invention, bamboo shoots refer to the young shoots of bamboo in the subfamily Bambusoideae of the family Poaceae. Although the preferred bamboo shoots used in the present invention vary depending on the species of bamboo, for example, in the case of Phyllostachys bambusoides, those harvested from June to September with a ground part of 10 to 500 cm are preferred, and those with a ground part of 30 cm to 200 cm are more preferred. Also, in the case of Phyllostachys pubescens, those harvested from early March to late June until young shoots emerge on the ground are preferred. If it grows beyond the above range, the edible part and skin of the young shoots become hard, and the amount of tyrosine, which is not preferable as a component of cosmetics, increases, making it unsuitable for the present invention.

[0020] The preparation of the extract can be carried out by contacting the bamboo shoot skin or edible part, after washing with water in advance to remove foreign substances if necessary, as it is or after drying, and then finely cutting or pulverizing if necessary, with an extraction solvent according to a conventional method such as the immersion method.

[0021] As the extraction solvent, the same extraction solvent as that of the above-mentioned rice bran extract can be used. When preparing the extract, there is no particular limitation on the pH of the extract, but it is preferably in the range of pH 2 to 8. Further, from the viewpoint of suppressing the dissolution of tyrosine, which is a component undesirable as a cosmetic, in the extract, it is more preferably in the range of pH 2 to 4. The adjustment of the pH is carried out by adding an acidic regulator such as lactic acid, citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. to the above-mentioned extraction solvent.

[0022] The extraction conditions such as the extraction temperature and extraction time vary depending on the type of solvent used and the pH. For example, in the case of the immersion method using water as the extraction solvent, the extraction temperature is generally in the range of 1 to 90°C, preferably 40 to 80°C, and the extraction time is generally in the range of 0.5 to 24 hours, preferably 0.5 to 6 hours when the extraction temperature is 40°C.

[0023] The extract obtained under the above conditions is generally adjusted to a pH of 4 to 8, and this can be used as it is as a component of a skin external preparation, or can be used at a desired concentration by concentration under reduced pressure or the like.

[0024] The present invention is a skin external preparation containing, as active ingredients, a hydrolyzate of the above-mentioned rice bran extract, a bamboo shoot extract, and niacinamide.

[0025] When formulating the active ingredient according to the present invention in a topical skin preparation, in addition to the hydrolyzate of rice bran extract, bamboo shoot extract, and niacinamide which are essential components, components usually used in topical skin preparations (topical pharmaceuticals, quasi-drugs, cosmetics), for example, oily components, surfactants (synthetic type, natural product type), emulsifiers, emulsification aids, moisturizers, anti-inflammatory agents, thickeners, preservatives and bactericides, anti-acne agents, cell activators, powder components, ultraviolet absorbers, antioxidants, whitening agents, pigments, fragrances, etc. can be appropriately formulated as needed.

[0026] Here, examples of the oily components include plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa butter, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, etc., such as plant-derived squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin, etc.; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane, etc.; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid, etc.; higher alcohols such as lauryl alcohol, cetyl alcohol, pantothenyl alcohol, stearyl alcohol, etc.; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (such as octyldodecyl stearate), etc.

[0027] As surfactants, for example, nonionic surfactants such as polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitol fatty acid ester, etc.; anionic surfactants such as fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc.; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N, N-dialkylmorpholinium salts, polyethylene polyamine fatty acid amide salts, etc.; amphoteric surfactants such as N, N-dimethyl-N-alkyl-N-carboxymethylammonio betaine, N, N, N-trialkyl-N-alkylene ammoniocarboxy betaine, N-acylamidopropyl-N′, N′-dimethyl-N′-β-hydroxypropylammonio sulfobetaine, etc. can be used.

[0028] As emulsifiers and / or emulsification aids, stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (such as sodium), complexes of sugar and protein, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soy-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzyme-treated stevia), silicates (such as aluminum, magnesium), carbonates (such as calcium, sodium), saponin and its derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented cereals (such as wheat, beans, miscellaneous grains), etc. can also be blended.

[0029] Examples of moisturizers include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, etc. Further examples include saccharides such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation broth, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, octyldodecyl higher fatty acid, seaweed extract, estradiol, various amino acids and their derivatives.

[0030] Examples of thickeners include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan, fucoidan, etc.; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, guar gum, etc.; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, etc.; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinyl pyrrolidone, acrylic acid-methacrylic acid copolymer, etc.; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, polyacrylic acid, etc.

[0031] Examples of anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives, etc.

[0032] Examples of antiseptic and bactericidal agents include urea; benzoic acid or its salts, paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, etc.; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisquinolinium bromide, resorcin, jamal (imidazodainyl urea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenyl ether, hinokitiol, 1,2 - pentanediol, propanediol, 50% concentrated benzalkonium chloride solution, essential oils such as peppermint oil, eucalyptus oil, etc., bark distillates, radish fermentation broth, ethanol derived from plants such as sugarcane, corn, etc., or 1,3 - butylene glycol, etc.

[0033] Examples of cell activators include pantothenyl alcohol, menthol, dl - menthol, and γ - oryzanol, etc.

[0034] Examples of anti - acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, etc.

[0035] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose - based powder, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybean, adzuki bean, etc.), etc.

[0036] Examples of ultraviolet absorbers include ethyl para - aminobenzoate, ethylhexyl para - dimethylaminobenzoate, amyl salicylate and its derivatives, 2 - ethylhexyl para - methoxycinnamate, octyl cinnamate, oxybenzone, 2,4 - dihydroxybenzophenone, 2 - hydroxy - 4 - methoxybenzophenone - 5 - sulfonate, 4 - tertiary - butyl - 4 - methoxybenzoylmethane, 2-(2 - hydroxy - 5 - methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, and the like.

[0037] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A or its derivatives (e.g., retinol palmitate), and the like.

[0038] Examples of skin - lightening agents include kojic acid or its derivatives, ascorbic acid or its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, resorcinol derivatives, potassium 4 - methoxysalicylate, vitamin E or its derivatives, nicotinic acid or its derivatives, magnolignan (5,5'-dipropyl - biphenyl - 2,2’ - diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α - hydroxy acid, AMP (adenosine monophosphate, adenosine 1 - phosphate), and one or more selected therefrom.

[0039] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate; kojic acid ethers; kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbate-2-phosphate, magnesium L-ascorbate-2-phosphate, sodium L-ascorbate-2-sulfate, and magnesium L-ascorbate-2-sulfate; ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid) and L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid); 6-position acylates of those ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.); L-ascorbic acid tetra-fatty acid esters such as L-ascorbic acid tetraisopalmitate and L-ascorbic acid tetralaurate; 3-O-ethylascorbic acid; sodium L-ascorbate-2-phosphate-6-O-palmitate. Examples of the hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of the resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of the 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivatives include nicotinamide and benzyl nicotinate. Examples of the vitamin E derivatives include vitamin E nicotinate and vitamin E linoleate. Examples of the α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0040] In the present invention, by using in combination the combination of fermented products according to the present invention and active ingredients of topical skin preparations such as niacinamide, dipotassium glycyrrhizinate, ascorbic acid or its derivatives, tranexamic acid, vitamin E or its derivatives, etc., it is also suggested that synergistic effects such as wrinkle improvement and whitening effect can be further exerted.

[0041] The application sites of the topical skin preparation (including cosmetics, quasi-drugs, and topical pharmaceuticals) containing the combination of fermented products according to the present invention include the entire skin including the scalp, and there is no particular limitation. Therefore, examples of the dosage form include, for example, emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushes, white powders, facial washes, body shampoos, soaps, mask preparations, and bath agents, etc., but the present invention is not limited thereto.

[0042] Next, the present invention will be further specifically described by production examples, formulation examples, examples, and test examples, but the present invention is not limited thereto. In the following, all parts are by weight, and all % are by weight.

[0043] Production Example 1. Hydrolysate of rice bran extract 1500 g of 0.1 M lactic acid aqueous solution was added to 500 g of rice bran, and after stirring to thoroughly mix the rice bran and the lactic acid aqueous solution, it was left standing at room temperature for 1 day. Next, the insoluble matter was removed by filtration, and the filtrate was treated with papain. The enzyme treatment was carried out by using 1.2 mg of the enzyme and holding it at 80 °C for 1 hour at the optimum pH of the enzyme. The insoluble matter generated by the treatment was filtered off, and the filtrate was adjusted to pH 6.5 with phytic acid to obtain 731 g of a pale yellow transparent rice bran extract hydrolysis solution (solid content concentration 3.7%).

[0044] Production Example 2. Bamboo shoot skin extract (1) 1000 g of purified water and 0.92 g of lactic acid were added to 100 g of the dried and pulverized product of the skin of Phyllostachys pubescens bamboo shoots, and extraction was carried out at 40 °C for 4 hours. The extract was filtered to obtain 532 g of a light brown bamboo shoot skin extract solution (solid content concentration 0.6%).

[0045] Production Example 3. Bamboo Shoots Skin Extract (2) 100 g of the dried and ground skin of bamboo shoots of Sasa albo-marginata was added to 1000 g of purified water, and extraction was carried out at 40 °C for 4 hours. The extract was filtered to obtain 521 g of a light brown bamboo shoots skin extract solution (solid content concentration: 0.5%).

[0046] Production Example 4. Bamboo Shoots Skin Extract (3) In Production Example 1, except that 100 g of the dried and ground skin of bamboo shoots of Phyllostachys bambusoides was used instead of Sasa albo-marginata, 640 g of a light brown transparent Phyllostachys bambusoides extract (solid content concentration: 0.6%) was obtained in the same process as in Production Example 2.

[0047] Test Example 1. Evaluation Test of Fibroblast Activation Effect Human dermal-derived fibroblasts NB1RGB were placed in a 96-well microplate containing Eagle's minimum essential medium containing 0.5% NCS at 1×10 4Seeds were sown individually or in holes, and pre-cultured for 1 day under the conditions of 37 °C and 5.0% CO₂. Then, as a sample solution, nicotinamide, the hydrolyzate of Production Example 1, and the extract of Production Example 2, which together form the sample of the present invention, were added to the medium. Here, nicotinamide was added to the medium so that its concentration was 1 mM, and for the mixed composition consisting of the combination of the hydrolyzate of Production Example 1 and the extract of Production Example 2, three concentrations were set such that the final concentration as a solution was 0.5% (A), 1.0% (B), and 2.0% (C), and these were added. After adding the sample of the present invention, it was cultured for an additional 3 days under the same conditions as the pre-culture. Next, the medium was removed, 0.03% MTT was added, and it was held at 37 °C for 1 hour. Then, the generated formazan was extracted with isopropanol, and the MTT value was measured at a wavelength of 570 - 630 nm using a microplate reader (Model 680, manufactured by Bio-Rad). Further, as a comparative control, a comparative test group (comparative samples 1A - 1C) when nicotinamide (1 mM) and the hydrolyzate of Production Example 1 [0.5% (A), 1.0% (B), 2.0% (C)] were added, a comparative test group (comparative samples 2A - 2C) when nicotinamide (1 mM) and the extract of Production Example 2 [0.5% (A), 1.0% (B), 2.0% (C)] were added, and a comparative test group (comparative sample 3) when only nicotinamide (1 mM) was added as the sample solution were set up, and the test was conducted by the same operation as above. Further, the same operation as above was also performed for a control (Control) where only the medium was added instead of the sample solution, and the relative values of the MTT values at the time of adding each of the sample of the present invention and comparative samples 1 - 3 with respect to the MTT value obtained here were determined, and the fibroblast MTT activity rate (%) was obtained.

[0048] The results of Test Example 1 are shown in Figure 1. As shown in Figure 1, for samples 1A - 1C of the present invention, a concentration-dependent cell respiration activity, that is, a fibroblast activation effect, was confirmed. Further, it was also confirmed that the fibroblast activation effect was higher compared to comparative samples 1A - 1C, comparative samples 2A - 2C, and comparative sample 3. That is, by combining the two components (mixed composition) of the hydrolyzate of rice bran extract and the extract of bamboo shoots with nicotinamide, a higher fibroblast activation effect was obtained compared to combining nicotinamide with the hydrolyzate of rice bran extract or the extract of bamboo shoots alone at the same concentration as the said mixed composition.

[0049] Test Example 2. Evaluation Test of Collagen Synthesis Promoting Effect Human dermal-derived fibroblasts NB1RGB were seeded at 1×10 4 cells / well in a 96-well microplate containing Eagle's minimum essential medium supplemented with 0.5% NCS, and pre-cultured for 1 day under the conditions of 37°C and 5.0% CO2. Then, as a sample solution, niacinamide, the hydrolyzate of Production Example 1, and the extract of Production Example 2 were added to the medium to form the sample 1 of the present invention. Here, niacinamide was added to the medium so that its concentration was 1 mM, and a mixed composition consisting of a combination of the hydrolyzate of Production Example 1 and the extract of Production Example 2 was added at three concentrations with final concentrations as solutions of 0.5% (A), 1.0% (B), and 2.0% (C) to set test groups. After adding Sample 1A - 1C of the present invention respectively, each medium was further cultured for 5 days under the same conditions as the pre-culture conditions. Next, the medium was removed, the cells were fixed with cold methanol and cold ethanol, and then stained with a saturated picric acid aqueous solution containing 0.1% Sirius Red. After washing with purified water, extraction was performed with a 0.1% NaOH:methanol = 1:1 solution, and the amount of collagen was measured at a wavelength of 540 nm using a microplate reader (Model 680, manufactured by Bio-Rad). Furthermore, as comparative controls, comparative test groups (comparative samples 1A - 1C) with niacinamide (1 mM) and the hydrolyzate of Production Example 1 [0.5% (A), 1.0% (B), 2.0% (C)] added, comparative test groups (comparative samples 2A - 2C) with niacinamide (1 mM) and the extract of Production Example 2 [0.5% (A), 1.0% (B), 2.0% (C)] added, and a comparative test group (comparative sample 3) with only niacinamide (1 mM) added as the sample solution were set, and the test was conducted in the same manner as above. Also, for the case without addition of a sample (Control) where the medium was added instead of the sample solution, the same operation as above was performed, and the relative value of the amount of collagen at the time of addition of each of the sample 1 of the present invention and comparative samples 1 - 3 to the amount of collagen obtained here was determined and taken as the collagen synthesis rate (%).

[0050] The results of Test Example 2 are shown in Fig. 2. As shown in Fig. 2, a concentration-dependent collagen synthesis promoting effect was confirmed for the samples 1A to 1C of the present invention. Also, it was confirmed that the samples 1A to 1C of the present invention had a higher collagen synthesis promoting effect compared to Comparative Samples 1A to 1C, Comparative Samples 2A to 2C, and Comparative Sample 3. That is, by combining the hydrolyzate of rice bran extract and the extract of bamboo shoots (mixed composition) with niacinamide, a higher collagen synthesis promoting effect can be obtained than by combining niacinamide with the hydrolyzate of rice bran extract or the extract of bamboo shoots at the same concentration as the mixed composition alone.

[0051] As described above, by combining niacinamide according to the present invention with the hydrolyzate of rice bran extract and the extract of bamboo shoots, based on the remarkable fibroblast activating effect and collagen synthesis promoting effect, it is possible to provide an active ingredient that improves the state of the dermis and exhibits an improving effect on wrinkles, sagging, etc.

[0052] Formulation Example 1. Lotion [Ingredients] Parts Eucalyptus oil 0.2 Polyoxyethylene (5.5) cetyl alcohol 5.0 Niacinamide 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Stearyl glycyrrhizinate 0.05 Isopropylmethylphenol 0.1 Allantoin 0.1 D-Pantothenyl alcohol 0.1 Salicylic acid 0.5 Urea 5.0 L-Menthol 0.9 DL-Menthol 0.2 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Hydrolyzate of Production Example 1 0.5 Extract of Production Example 2 0.5 Methylparaben 0.1 Thymol 0.003 Photosensitizer No. 201 0.002 Purified water in an amount such that the total amount is 100 parts

[0053] Formulation Example 2. Lotion [Ingredients] parts Glyceryl caprylate 3.0 Polyglyceryl-10 laurate 3.0 Cetanol 2.0 Behenyl alcohol 2.0 Methylparaben 0.1 Niacinamide 5.0 Ascorbic acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc oxide 2.0 Hydrolysate of Production Example 1 1.0 Extract of Production Example 3 1.0 dl-Camphor 0.5 Glycerin 2.0 1,3-Butylene glycol 5.0 Potassium hydroxide 0.5 Purified water in an amount such that the total amount is 100 parts

[0054] Formulation Example 3. Lotion [Ingredients] parts Jojoba oil 1.0 Polyoxyethylene (5.5) cetyl alcohol 5.0 Methylparaben 0.1 Niacinamide 5.0 Ascorbic acid glucoside 2.0 Tranexamic acid 2.0 ε-Aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water in an amount such that the total amount is 100 parts

[0055] Formulation Example 4. Emulsion [Ingredients] parts Squalane 5.0 Cyclopentasiloxane 1.0 Hexanal 3.0 Hexyldecyl isostearate 1.0 Glyceryl tri(caprylate / caprate) 1.0 Polyglyceryl-10 laurate 5.0 Polyglyceryl-10 isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 Ascorbic acid glucoside 2.0 Arbutin 3.0 Potassium hydroxide 0.5 Hydrolyzate of Production Example 1 1.0 Extract of Production Example 2 1.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethyl cellulose 0.3 Xanthan gum 0.2 Silkworm polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Sodium Hyaluronate 1.0 Purified water in an amount such that the total amount is 100 parts

[0056] Formulation Example 5. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of Magnesium L - Ascorbyl Phosphate were used instead of 2.0 parts of Ascorbyl Glucoside in the components of Formulation Example 4.

[0057] Formulation Example 6. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of 3 - O - Ethyl Ascorbic Acid were used instead of 2.0 parts of Ascorbyl Glucoside in the components of Formulation Example 4.

[0058] Formulation Example 7. Cream [Ingredients] parts Olive Oil 5.0 Jojoba Oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Di(octyldodecyl / phytosteryl / behenyl) Lauroyl Glutamate 5.0 Glyceryl Caprylate 1.0 Glyceryl Stearate 1.0 Isostearyl Glyceryl 3.0 Niacinamide 5.0 γ - Oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic Acid 2.5 D - Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium Chloride 0.05 Urea 3.0 β - Glycyrrhetinic Acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium Glycyrrhetinate 0.1 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Lactic acid bacteria-fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysophosphatidylcholine 0.5 Oil-soluble carrot extract 2.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-Camphor 0.3 l-Menthol 0.5 Purified water in an amount such that the total amount is 100 parts

[0059] Prescription Example 8. Pack [Ingredients] parts Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Niacinamide 5.0 Xanthan gum 2.0 Polyglyceryl-6 myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated lecithin 3.0 Hydrolyzed lecithin 3.0 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Purified water in an amount such that the total amount is 100 parts

[0060] Formulation Example 9. Hair Shampoo [Ingredients] Parts Sodium lauryl sulfate 10.0 Glyceryl monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl alcohol 2.0 Behenyl alcohol 2.0 Dimethicone 3.0 Niacinamide 5.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Zinc pyrithione 0.3 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water in an amount such that the total is 100 parts

[0061] Formulation Example 10. Hair Conditioner [Ingredients] Parts Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Niacinamide 5.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Zinc pyrithione 0.3 Hydrolysate of Production Example 1 1.0 Extract of Production Example 4 1.0 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water in an amount such that the total is 100 parts

[0062] Formulation Example 11. Cosmetic for washing [Ingredients] parts Potassium cocoyl glycinate 5.0 Glycerin 10.0 Glyceryl caprylate 1.0 Sodium lauroyl aspartate 10.0 Niacinamide 5.0 Cetanol 3.0 Myristyl alcohol 3.0 Isopropylmethyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Triclosan 0.1 Triclocarban 0.5 Triclocarban 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Purified water in an amount such that the total amount is 100 parts

[0063] Formulation Example 12. Sheet Mask Impregnate the non-woven fabric with the following components to obtain a sheet mask. [Components] parts Glycerin 3.0 1,3-Butylene glycol 2.0 L-Ascorbic acid 2-glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Ammophila extract 1.0 Niacinamide 5.0 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Potassium hydroxide appropriate amount Purified water in an amount such that the total amount is 100 parts

[0064] Formulation Example 13. Skin Lotion [Components] parts Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Hyaluronic acid hydrolyzate 1.0 Lactic acid bacteria culture 1.0 Niacinamide 5.0 Hydrolysate of Production Example 1 1.0 Extract of Production Example 2 1.0 Citric acid 0.3 Sodium citrate 0.6 Purified water in an amount such that the total amount is 100 parts

[0065] Formulation Example 14. Anti-Wrinkle Cream [Components] parts Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba wax 1.0 Shea butter 2.0 Behenyl alcohol 1.0 Stearyl alcohol 1.5 Candelilla wax 0.5 Niacinamide 5.0 Lactic acid bacteria-fermented rice 3.0 Hydrolyzate of Production Example 1 1.0 Extract of Production Example 2 1.0 Hydrogenated lecithin 2.0 Extract of Catalpa ovata G. Don 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide appropriate amount Purified water an amount such that the total amount is 100 parts

[0066] Formulation Example 15. Wrinkle-improving cream Among the components of Formulation Example 14, a wrinkle-improving cream was obtained in the same manner as Formulation Example 14, except that 1.0 part of the extract of Production Example 3 was used instead of 1.0 part of the extract of Production Example 2.

[0067] Formulation Example 16. Wrinkle-improving cream Among the components of Formulation Example 15, a wrinkle-improving cream was obtained in the same manner as Formulation Example 15, except that 1.0 part of the extract of Production Example 4 was used instead of 1.0 part of the extract of Production Example 2.

Claims

1. A fibroblast activator containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients.

2. A collagen synthesis promoter containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients.

3. A topical skin preparation containing niacinamide, a hydrolyzate of rice bran extract, and a bamboo shoot extract as active ingredients.

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