Whitening composition and method for producing the same

Abstract

To provide a skin-whitening composition and a method for producing the same.SOLUTION: A method for producing a skin-whitening composition includes the steps of: extracting Morus alba root bark with a first alcohol liquid to yield a Morus alba root bark alcohol extract; extracting Glechoma hederacea with a second alcohol liquid to yield a Glechoma hederacea alcohol extract; extracting Michelia alba flower with a third alcohol liquid to yield a Michelia alba flower alcohol extract; and mixing the Morus alba root bark alcohol extract, Glechoma hederacea alcohol extract, Michelia alba flower alcohol extract, a humectant, and a solvent to create a skin-whitening composition.SELECTED DRAWING: Figure 5

Description

[Technical Field]

[0001] The present disclosure relates to a whitening composition and a method for producing the same, and more particularly to a whitening composition containing a plant extract and a method for producing the same. [Background technology]

[0002] Factors such as ultraviolet radiation, endocrine influences, and friction stimulate melanocytes to produce melanin, resulting in skin blemishes. However, the love of beauty is human nature, and having beautiful, fair, and blemish-free skin is a dream pursued by many consumers. In Asian cultures, in particular, fair skin has long been associated with youth and beauty. Skin-whitening products are popular among consumers due to their benefits, such as reducing pigmentation, mitigating blemishes, evening out skin tone, and removing blemishes.

[0003] However, current skin whitening products still suffer from the following drawbacks: Many conventional skin whitening products designed to reduce dark spots focus on removing superficial dark spots in the epidermis. However, melanin is formed in the middle and deep (basal) layers of the epidermis, and if only focusing on removing dark spots in the epidermis, dark spots will quickly reappear. Furthermore, currently available skin whitening ingredients, such as hydroquinone, kojic acid, arbutin, ellagic acid, and vitamin C (ascorbic acid) and its derivatives, are prone to skin irritation. For example, while hydroquinone can quickly whiten the skin, it is prone to skin irritation and hypersensitivity, resulting in side effects such as dermatitis, erythema, burns, leukoplakia, and irregular skin depigmentation. Therefore, hydroquinone has been banned from use in cosmetics by the Ministry of Health. For example, skin-whitening ingredients such as fruit acids and salicylic acid tend to cause local skin irritation, such as redness, peeling, dryness, itching, and a burning sensation.

[0004] In view of the above, there is a need for the development of a whitening product that has an excellent whitening effect and is also low in irritation. Summary of the Invention

[0005] The present disclosure provides a skin whitening composition comprising an alcohol extract of mulberry bark (Morus alba root bark), an alcohol extract of Glechoma hederacea, an alcohol extract of Michelia alba flower, a moisturizer, and a solvent.

[0006] In some embodiments, the weight ratio of the mulberry bark alcohol extract, the kakidooshi (Prunus persica) alcohol extract, and the ginkgo bark alcohol extract is 30-95:1-20:1-20.

[0007] In some embodiments, the total weight of the mulberry bark alcohol extract, the kakidoshuoshō alcohol extract, and the ginkgo bark alcohol extract is 0.01 wt% to 20 wt%, based on 100 wt% of the total weight of the whitening composition.

[0008] In some embodiments, the mulberry bark alcohol extract is obtained by extracting mulberry bark with a first alcohol liquid, the ginkgo bark alcohol extract is obtained by extracting ginkgo bark with a second alcohol liquid, and the ginkgo bark alcohol extract is obtained by extracting ginkgo bark with a third alcohol liquid, and the first alcohol liquid, the second alcohol liquid, and the third alcohol liquid each contain an alcohol having 1 to 10 carbon atoms.

[0009] In some embodiments, the first alcohol liquid, the second alcohol liquid, and the tertiary alcohol liquid each comprise methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol, n-pentanol, 3-methylbutanol, 2-methylbutanol, 2,2-dimethylpropanol, 2-pentanol, 3-pentanol, 3-methyl-2-butanol, 2-methyl-2-butanol, hexanol, heptanol, octanol, nonanol, decanol, ethylene glycol, 1,2-propanediol, 1,4-butanediol, 1,6-hexanediol, neopentyl glycol, trimethylolpropane, glycerin, or a combination thereof.

[0010] In some embodiments, the whitening composition further comprises a skin conditioner, an antioxidant, a thickener, an emulsifier, an emulsion stabilizer, a fragrance, an emollient, or a combination thereof.

[0011] In some embodiments, the whitening composition is an essence, essence cream, eye cream, face cream, hand cream, lotion, mask, toner, sunscreen, or barrier cream.

[0012] The present disclosure provides a method for producing a whitening composition, including the steps of extracting mulberry bark with a first alcoholic liquid to obtain a mulberry bark alcoholic extract, extracting ginkgo bark with a second alcoholic liquid to obtain a ginkgo bark alcoholic extract, extracting marigold with a third alcoholic liquid to obtain a ginkgo bark alcoholic extract, and mixing the mulberry bark alcoholic extract, the marigold alcoholic extract, the ginkgo bark alcoholic extract, a moisturizer, and a solvent to form a whitening composition.

[0013] In some embodiments, the first alcohol liquid, the second alcohol liquid, and the tertiary alcohol liquid each comprise methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol, n-pentanol, 3-methylbutanol, 2-methylbutanol, 2,2-dimethylpropanol, 2-pentanol, 3-pentanol, 3-methyl-2-butanol, 2-methyl-2-butanol, hexanol, heptanol, octanol, nonanol, decanol, ethylene glycol, 1,2-propanediol, 1,4-butanediol, 1,6-hexanediol, neopentyl glycol, trimethylolpropane, glycerin, or a combination thereof.

[0014] In some embodiments, the first alcohol liquid comprises a first alcohol having a volume percentage concentration of 50% to 100%, the second alcohol liquid comprises a second alcohol having a volume percentage concentration of 50% to 100%, and the tertiary alcohol liquid comprises a tertiary alcohol having a volume percentage concentration of 50% to 100%.

[0015] In some embodiments, the first alcoholic liquid comprises a first alcohol and water, the second alcoholic liquid comprises a second alcohol and water, and the tertiary alcoholic liquid comprises a tertiary alcohol and water.

[0016] In some embodiments, mulberry bark is extracted with the first alcoholic liquid, kakidooshi with the second alcoholic liquid, and ginkgo bark with the third alcoholic liquid, each at a temperature of 20°C to 70°C.

[0017] In some embodiments, the weight ratio of mulberry bark to the first alcoholic liquid is 1:10 to 1:20, the weight ratio of kakidooshi to the second alcoholic liquid is 1:10 to 1:20, and the weight ratio of ginkgo bark to the tertiary alcoholic liquid is 1:10 to 1:20.

[0018] In some embodiments, the time for extracting mulberry bark with the first alcoholic liquid is 12 to 96 hours, the time for extracting kakidooshi with the second alcoholic liquid is 12 to 96 hours, and the time for extracting ginkgo bark with the third alcoholic liquid is 12 to 96 hours. [Brief explanation of the drawings]

[0019] A more complete understanding of the present disclosure can be obtained by reading the following detailed description of the embodiments and by referring to the drawings, in which: [Figure 1] 1 shows the results of a skin irritation test for Example 1 of the present disclosure, a control group, and a positive control group. [Figure 2] 1 shows the results of an eye irritation test for Example 1 of the present disclosure, a control group, and a positive control group. [Figure 3] 1 shows the results of improving surface spots with an essence according to an embodiment of the present disclosure and a commercially available whitening product. [Figure 4] 1 shows the results of improvement of mid-layer spots by an essence according to an example of the present disclosure and a commercially available whitening product. [Figure 5] 1 shows the results of improving deep dark spots with an essence according to an embodiment of the present disclosure and a commercially available whitening product. DETAILED DESCRIPTION OF THE INVENTION

[0020] The following embodiments will be described in detail with reference to the drawings. For clarity, many practical details will be described in the following description. However, it should be understood that these practical details are not intended to limit the present disclosure. That is, in some embodiments of the present disclosure, these practical details are not necessary. In addition, to simplify the drawings, some well-known structures and elements are shown in the drawings in a schematic manner.

[0021] In this specification, ranges expressed as "a certain value to another value" are used as generalizations to avoid listing every single value within the range in the specification. Thus, the recitation of a particular numerical range includes any value within that numerical range and any smaller numerical range defined by any value within that numerical range, just as if such value and smaller numerical range were expressly recited in the specification.

[0022] Human skin color is determined by melanin, which is produced by melanocytes in the skin. Melanin is primarily synthesized in melanosomes in the skin. Melanogenesis is a complex chemical reaction involving enzymes, resulting in the production of eumelanin and pheomelanin, collectively known as L-dopa melanin. Melanin production begins with the oxidation of L-tyrosine by tyrosinase (TYR) (the rate-determining step) to produce L-dopaquinone (DQ). After DQ is produced, it becomes a substrate for the production of eumelanin and pheomelanin through intramolecular cyclization and oxidation. The regulatory mechanisms driving this synthesis are now known to involve three cell signaling pathways: (1) melanocortin-1 receptor (MC1R) signaling, (2) Wnt / β-catenin signaling, and (3) tyrosine kinase receptor (TKR) / stem cell factor (SCF) signaling. A common feature of these cell signaling pathways is the upstream master regulator microphthalmia-associated transcription factor (MITF), which activates TYR.Taking the common MC1R messaging example, α-MSH is a precursor polypeptide derived from pro-opiomelanocortin. Upon binding to MC1R, it activates downstream adenylyl cyclase, catalyzing the production of cyclic adenosine monophosphate (cAMP), and promotes the expression of TYR and tyrosinase-related protein-1 / 2 (TRP-1 / 2), resulting in increased melanin production.

[0023] Therefore, the whitening mechanism includes the following: (1) Preventing stimulation of melanocytes. When skin is damaged, an inflammatory response occurs, and inflammatory factors affect melanin production. Therefore, melanin production can be reduced by inhibiting the production of inflammatory melanin-promoting factors (e.g., prostaglandin E2 (PEG2)). (2) Inhibiting melanin formation. Blocking signals that produce melanin (e.g., α-melanocyte-stimulating hormones (α-MSH)) and inhibiting the activity of melanin-forming enzymes (e.g., TYR). (3) Inhibiting melanin transfer. Reducing melanin accumulation in the stratum corneum improves whitening effects.

[0024] The present disclosure provides a whitening composition containing a natural plant extract and a method for producing the whitening composition. The whitening composition contains an alcohol extract of mulberry bark, an alcohol extract of kakido oak, an alcohol extract of ginkgo tree, a moisturizer, and a solvent. The whitening composition of the present disclosure has the triple whitening effects of antioxidant, anti-inflammatory, and tyrosinase activity inhibition, and also has the advantages of low skin and eye irritation. This whitening composition can effectively improve blemishes in the superficial layer (stratum corneum) of human skin, as well as in the middle and deep layers (basal layer), improve melanin deposition, and achieve excellent whitening effects by reducing blemishes. The whitening composition of the present disclosure can be applied to skin care products, cosmetics, or pharmaceuticals. In some embodiments, the moisturizer includes 1,3-butylene glycol, sodium hyaluronate, glycerin, inositol, trehalose, propylene glycol, sorbitol, 1,2,6-hexanetriol, or a combination thereof. In some embodiments, the solvent is a polar solvent such as, for example, water or an alcohol.

[0025] In some embodiments, the weight ratio of the mulberry bark alcohol extract, the kakido oshio extract, and the ginkgo bark alcohol extract is 30-95:1-20:1-20, for example, 40-50:2-5:1-5. In some embodiments, based on 100 wt% of the total weight of the whitening composition, the total weight of the mulberry bark alcohol extract, the kakido oshio extract, and the ginkgo bark alcohol extract is 0.01 wt%-20 wt%, for example, 0.01, 0.02, 0.03, 0.04, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, or 20 wt%. A whitening composition having the above weight ratio range and / or weight percentage range has excellent triple whitening effects of antioxidant, anti-inflammatory, and tyrosinase activity inhibition, and is less likely to irritate the skin and eyes. In some embodiments, the moisturizer is 1 wt% to 25 wt%, based on 100 wt% of the total weight of the whitening composition.

[0026] In some embodiments, the whitening composition further comprises a cosmetically or physiologically acceptable medium, such as, but not limited to, a skin conditioner, antioxidant, thickener, emulsifier, emulsion stabilizer, fragrance, emollient, or combination thereof. In some embodiments, the skin conditioner comprises 1,2-hexanediol, arginine, allantoin, panthenol, octamethylpolysiloxane, squalane, tocopheryl acetate, arginine, or combinations thereof. In some embodiments, the antioxidant comprises p-hydroxyacetophenone. In some embodiments, the thickener comprises acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sodium acrylic acid (ester) copolymer, carbomer, xanthan gum, hydroxyethyl cellulose, or combinations thereof. In some embodiments, the emulsifier is a nonionic, anionic, or amphoteric surfactant, such as polysorbate-60, sorbate isostearate, glyceryl stearate, polyethylene glycol-75 stearate (PEG-75 stearate), ceteth-20, steareth-20, lecithin, cetearyl olivate, sorbitan olivate, or a combination thereof. In some embodiments, the emulsion stabilizer includes behenyl alcohol, cetyl alcohol, or a combination thereof. In some embodiments, the emollient includes isononyl isononanoate, polydimethylsiloxane, tri(ethylhexanoate), isohexadecane, caprylic / capric triglyceride, ethylhexyl palmitate, or a combination thereof.

[0027] In some embodiments, the whitening composition is, but is not limited to, an essence, an essence cream, an eye cream, a face cream, a hand cream, a lotion, a mask, a toner, a sunscreen, or a barrier cream.

[0028] In some embodiments, the skin lightening composition is an essence, and the essence comprises an alcohol extract of mulberry bark, kakido-oshi and ginkgo tree, a moisturizer, a solvent, and a thickener. In some embodiments, the essence further comprises a skin conditioner, an antioxidant, an emulsifier, a fragrance, or a combination thereof.

[0029] In some embodiments, the skin lightening composition is an eye cream, and the eye cream comprises an alcohol extract of mulberry bark, kakido-oshi and ginkgo tree, a moisturizer, a solvent, an emulsifier, and an emollient, hi some embodiments, the eye cream further comprises a skin conditioner, an antioxidant, an emulsion stabilizer, a thickener, a fragrance, or a combination thereof.

[0030] In some embodiments, the skin lightening composition is an emulsion, and the emulsion comprises an alcohol extract of mulberry bark, kakido-oshi and ginkgo tree, a moisturizer, a solvent, an emulsifier, and an emollient, hi some embodiments, the emulsion further comprises a skin conditioner, an antioxidant, an emulsion stabilizer, a thickener, an essence, or a combination thereof.

[0031] In some embodiments, the skin lightening composition is a mask comprising an alcohol extract of mulberry bark, cinnamon bark, and ginkgo tree, a moisturizer, a solvent, and a thickener. In some embodiments, the mask further comprises a skin conditioner, an antioxidant, an emulsifier, a fragrance, or a combination thereof.

[0032] In some embodiments, the skin lightening composition is a lotion, and the lotion comprises an alcohol extract of mulberry bark, kakido-oshi and ginkgo tree, a moisturizer, and a solvent. In some embodiments, the lotion further comprises a skin conditioner, an antioxidant, an emulsifier, a fragrance, or a combination thereof.

[0033] A method for preparing a whitening composition includes extracting mulberry bark with a first alcoholic liquid to obtain a mulberry bark alcoholic extract, extracting ginkgosi fruit with a second alcoholic liquid to obtain a ginkgosi fruit alcoholic extract, extracting ginkgo bark with a third alcoholic liquid to obtain a ginkgo bark alcoholic extract, and mixing the mulberry bark alcoholic extract, the ginkgosi fruit alcoholic extract, the ginkgo bark alcoholic extract, a moisturizer, and a solvent to form a whitening composition. In some embodiments, the extraction steps of mulberry bark, ginkgosi fruit, and ginkgo bark are carried out simultaneously, and the first, second, and third alcoholic liquids are the same. In some embodiments, the weight ratio of the total weight of mulberry bark, kakidoushi, and ginkgo bark to the total alcoholic liquid is 1:10 to 1:20. In other words, the solid-liquid ratio is 1:10 to 1:20, for example, 1:10, 1:12, 1:14, 1:16, 1:18, or 1:20. In some embodiments, the weight ratio of mulberry bark, kakidoushi, and ginkgo bark is 100-250:1-20:1-20. In some embodiments, the weight of mulberry bark is greater than the weight of kakidoushi. In some embodiments, the weight of kakidoushi is greater than the weight of ginkgo bark. For example, the extraction rate of mulberry bark is about 4.0%, the extraction rate of kakidoushi is about 6.5%, and the extraction rate of ginkgo bark is about 20.0%.

[0034] In some embodiments, the weight ratio of mulberry bark to the first alcoholic liquid is 1:10 to 1:20, for example, 1:10, 1:12, 1:14, 1:16, 1:18, or 1:20. The weight ratio of kakidooshi to the second alcoholic liquid is 1:10 to 1:20, for example, 1:10, 1:12, 1:14, 1:16, 1:18, or 1:20. The weight ratio of ginkgo bark to the third alcoholic liquid is 1:10 to 1:20, for example, 1:10, 1:12, 1:14, 1:16, 1:18, or 1:20.

[0035] In some embodiments, the first alcohol liquid, the second alcohol liquid, and the third alcohol liquid each comprise an alcohol having 1 to 10 carbon atoms, such as an alcohol having 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 carbon atoms. In some embodiments, the alcohol comprises a monohydric alcohol, a dihydric alcohol, or a combination thereof. In some embodiments, the first alcohol liquid, the second alcohol liquid, and the tertiary alcohol liquid each comprise methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol, n-pentanol, 3-methylbutanol, 2-methylbutanol, 2,2-dimethylpropanol, 2-pentanol, 3-pentanol, 3-methyl-2-butanol, 2-methyl-2-butanol, hexanol, heptanol, octanol, nonanol, decanol, ethylene glycol, 1,2-propanediol, 1,4-butanediol, 1,6-hexanediol, neopentyl glycol, trimethylolpropane, glycerin, or a combination thereof.

[0036] In some embodiments, the first alcoholic liquid comprises a first alcohol and a solvent, such as, but not limited to, water. In some embodiments, the first alcoholic liquid comprises only alcohol. In some embodiments, the second alcoholic liquid comprises a second alcohol and a solvent, such as, but not limited to, water. In some embodiments, the second alcoholic liquid comprises only alcohol. In some embodiments, the third alcoholic liquid comprises a tertiary alcohol and a solvent, such as, but not limited to, water. In some embodiments, the third alcoholic liquid comprises only alcohol.

[0037] In some embodiments, the first alcohol liquid includes a first alcohol and the volume percentage of the first alcohol is 50% to 100%, for example, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100%. The second alcohol liquid includes a second alcohol and the volume percentage of the second alcohol is 50% to 100%, for example, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100%. The third alcohol liquid includes a tertiary alcohol and the volume percentage of the tertiary alcohol is 50% to 100%, for example, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100%.

[0038] In some embodiments, mulberry bark is extracted with the first alcoholic liquid, kakidooshi with the second alcoholic liquid, and ginkgo tree with the third alcoholic liquid, each at a temperature of 20°C to 70°C, for example, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70°C. In some embodiments, the time for extracting mulberry bark with the first alcoholic liquid is 12 to 96 hours, for example, 12, 24, 36, 48, 60, 72, 84, or 96 hours. In some embodiments, the time for extracting kakidooshi with the second alcoholic liquid is 12 to 96 hours, for example, 12, 24, 36, 48, 60, 72, 84, or 96 hours. In some embodiments, the time for extracting the marigold with the tertiary alcoholic liquid is 12 to 96 hours, for example, 12, 24, 36, 48, 60, 72, 84, or 96 hours. The above temperature and / or extraction time ranges can effectively extract the whitening components in the mulberry bark, the hollyhock root, and the marigold, thereby obtaining a mixed plant extract with triple whitening effects of antioxidant, anti-inflammatory, and tyrosinase activity inhibition.

[0039] The features of the present disclosure will be described in more detail below with reference to Experimental Examples 1 to 9. The following examples will be described, but the materials used, their amounts and proportions, processing details, processing flow, etc. may be changed as appropriate without departing from the spirit of the present disclosure. Therefore, the present disclosure should not be interpreted as being limited by the following embodiments.

[0040] Experimental Example 1: Preparation of plant alcohol extracts

[0041] In Comparative Example 1, the method for producing the mulberry bark alcohol extract includes the steps of crushing and drying the mulberry bark, then mixing the mulberry bark with 95% alcohol by volume in a solid-liquid ratio of 1:15, soaking the mulberry bark at room temperature (25°C) and stirring for 72 hours, performing solid-liquid separation, concentrating the filtrate, and then freeze-drying to obtain the mulberry bark alcohol extract of Comparative Example 1.

[0042] In Comparative Example 2, the method for preparing the mulberry bark alcohol extract includes the steps of mixing dried whole mulberry plant with 95% alcohol by volume in a solid-liquid ratio of 1:15, soaking the mulberry bark at room temperature (25°C) and stirring for 72 hours, separating the solid and liquid, concentrating the filtrate, and then freeze-drying it to obtain the mulberry bark alcohol extract of Comparative Example 2.

[0043] In Comparative Example 3, the method for preparing the mulberry bark alcohol extract includes the steps of mixing dried ginkgo bark with 95% alcohol by volume in a solid-liquid ratio of 1:15, soaking the ginkgo bark at room temperature (25°C) and stirring for 72 hours, separating the solid from the liquid, concentrating the filtrate, and then freeze-drying it to obtain the ginkgo bark alcohol extract of Comparative Example 3.

[0044] In Example 1, the method for preparing the mixed alcohol extract of mulberry bark, kakidoushi, and ginkgo bark includes the steps of grinding dried mulberry bark, kakidoushi, and ginkgo bark in a 220:8:1 weight ratio to obtain plant pieces, mixing the plant pieces with 95% volumetric alcohol in a solid-liquid ratio of 1:15, soaking the plant pieces at room temperature (25°C) and stirring for 72 hours, separating the solids and liquids, concentrating the filtrate, and freeze-drying it to obtain the mixed alcohol extract of mulberry bark, kakidoushi, and ginkgo bark of Example 1. The extraction yields of mulberry bark, kakidoushi, and ginkgo bark were approximately 4.0%, 6.5%, and 20.0%, respectively. The weight ratio of the alcohol extracts of mulberry bark, kakidooshi and ginkgo tree is about 44:2.6:1.

[0045] Experimental Example 2: Tyrosinase activity inhibition test

[0046] In Experimental Example 2, the inhibitory effects of the mixed alcohol extract of mulberry bark, cinnamon bark, and ginkgo bark of Example 1 and kojic acid on tyrosinase activity were tested. The test results are shown in Table 1 below. [Table 1]

[0047] Since tyrosinase is a key enzyme in the process of melanin production in the skin, effectively reducing the activity of tyrosinase can reduce melanin production and achieve skin whitening effects. Kojic acid is a well-known commercially available whitening ingredient. As can be seen from Table 1, the IC of the extract of Example 1 50 The value is the IC of kojic acid 50 , which indicates that the extract of Example 1 has a high inhibitory effect on tyrosinase. Therefore, the inhibitory effect of the extract of Example 1 on melanin is superior to that of kojic acid. Specifically, the inhibitory effect of the extract of Example 1 is five times that of kojic acid.

[0048] Experimental Example 3: Antioxidant test, anti-inflammatory test, and tyrosinase activity inhibition test

[0049] In Experimental Example 3, the extracts of Comparative Examples 1, 2, 3, and Example 1 were subjected to a water-soluble antioxidant test, a fat-soluble antioxidant test, a test for inhibiting the inflammatory melanin-promoting factor PGE2, and a test for inhibiting tyrosinase. The test results are shown in Table 2 below.

[0050] The water-soluble antioxidant test involves the following steps. First, prepare an ABTS radical solution using equal volumes of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid; ABTS) (14 mM) and potassium persulfate (4.9 mM). Store the solution at room temperature for 16 hours, protected from light. ABTS is oxidized by the action of oxidizing agents to form green ABTS. In the presence of antioxidants, the generation of ABTS is suppressed. The absorbance of ABTS at 734 nm can be measured to calculate the total antioxidant capacity of the sample. Before using the ABTS radical solution, adjust the OD734 absorbance value of this solution to approximately 0.7. The solution was diluted to a concentration of 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL. For the test, 100 μL of the extract solution of Example 1 or 100 μL of the extract solutions of Comparative Examples 1 to 3 at concentrations of 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL was added to a 96-well microplate containing 100 μL of ABTS radical solution. After 20 minutes of reaction, the absorbance values ​​were recorded at OD734 using a SpectraMax M2 multifunction microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).

[0051] The fat-soluble antioxidant test involves the following steps: 50 μL of the extract solution of Example 1 at concentrations of 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL, or 50 μL of the extract solutions of Comparative Examples 1 to 3 at concentrations of 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL, is added to a 96-well microplate containing 150 μL of 1,1-diphenyl-2-trinitrophenylhydrazine (di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium; DPPH) (200 μM). After 15 minutes of reaction, absorbance values ​​are recorded at a wavelength of 515 nm using a SpectraMax M2 multifunction microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).

[0052] The inhibition test for the inflammatory melanin-promoting factor PGE2 involves the following steps: First, HaCaT cells are seeded into 12-well culture plates (1.5 x 105 / well) and cultured for 18 to 20 hours to allow stable adherent growth. The next day, the extract solution of Example 1 or the extract solutions of Comparative Examples 1 to 3 at concentrations of 7.8, 15.6, 31.3, 62.5, 125, 250, or 500 μg / mL are added to the culture plates and incubated for 12 hours. After incubation, the cells are incubated with tumor necrosis factor-α (TNF-α) (10 ng / mL) and interferon-γ (IFNγ) (100 U / mL) for 48 hours. Finally, the supernatant is collected and the PGE2 content is measured using an ELISA (R&D Systems).

[0053] The tyrosinase inhibition test involves the following steps. This test is performed in a 96-well microplate. First, 50 μL of tyrosinase solution (10 U in phosphate buffered saline (PBS)) is mixed with 50 μL of the extract solution of Example 1 at a concentration of 0.975, 1.95, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL, or 50 μL of the extract solutions of Comparative Examples 1 to 3 at a concentration of 0.975, 1.95, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μg / mL. The mixture is then incubated at 37°C for 15 minutes, and 100 μL of tyrosine solution is added to the mixture until the final concentration reaches 1 mM. After 15 minutes of reaction, the absorbance values ​​were recorded at a wavelength of 492 nm using a SpectraMax M2 multifunction microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) to evaluate the activity of tyrosinase and the inhibitory activity of the test substances. [Table 2]

[0054] As shown in Table 2 above, the extract of Example 1 has triple whitening effects of antioxidant, anti-inflammatory, and tyrosinase activity inhibition. The extract of Example 1 has a more comprehensive whitening effect than the extracts of Comparative Examples 1, 2, and 3.

[0055] Experimental Example 4: Safety Evaluation

[0056] In this example, a safety evaluation test is carried out using an in vitro bionic skin platform, and the extract of Example 1 is subjected to a skin irritation test and an eye irritation test.

[0057] The skin irritation test includes the following steps: This test is performed using EpiDerm, which is recognized by OECD TG 439. TMThe test was conducted using the SIT (EPI-200-SIT) human skin model. The extract of Example 1 was dissolved in glycerin to a final concentration of 2 wt% and then directly applied to a portion of the skin model surface. After 60 minutes of incubation, the extract was washed and the skin model was cultured for 42 hours. Finally, a cell viability assay (MTT assay) was used to evaluate the effect on cell activity of Example 1 or the positive control group (5 wt% SDS). The irritation assessment criteria were based on UN GHS (Type 2), with cell viability of 50% or less considered to be skin irritant. See Figure 1 for the test results. Figure 1 shows the skin irritation test results for Example 1, the control group, and the positive control group of the present disclosure. Experimental data are expressed as mean ± standard deviation (mean ± SD), with three samples per group.

[0058] The eye irritation test includes the following steps: This test is based on the EpiOcular method recognized by OECD TG 492. TM The test was conducted using the EIT (OCL-200-EIT) human corneal epithelium model. The extract from Example 1 was dissolved in glycerin to a final concentration of 2 wt% and then applied directly to a portion of the surface of the corneal epithelium model. After 30 minutes of incubation, the extract was washed and the epithelium model was cultured for 120 hours. Finally, the effect of Example 1 or the positive control group (5 wt% methyl acetate) on cellular activity was evaluated using a cell viability assay (MTT). The irritation assessment criteria were based on the UN GHS (Type 2: Irritant or No Category), with cell viability of 60% or less considered to be ocular irritant. See Figure 2 for test results. Figure 2 shows the eye irritation test results for Example 1, the control group, and the positive control group of the present disclosure. Experimental data are expressed as mean ± standard deviation (SD), with two samples per group.

[0059] As a result of the skin irritation test, as shown in Figure 1, the survival rate of the control group was 100.0 ± 3.0%, the survival rate of Example 1 was 74.5 ± 5.9%, and the survival rate of the positive control group was 3.2 ± 0.3%. As a result of the eye irritation test, as shown in Figure 2, the survival rate of the control group was 100.0 ± 0.9%, the survival rate of Example 1 was 104.9 ± 2.8%, and the survival rate of the positive control group was 36.1 ± 1.1%. As is clear from Figures 1 and 2, the extract of Example 1 was non-irritating in both the skin irritation test and the eye irritation test.

[0060] Experimental Example 5: Essence preparation and human skin whitening effect test

[0061] Please refer to Table 3 below for the range of ingredients and content of the essence, and the content of each ingredient in the essence of Example 2. Please refer to Example 1 above for the preparation process of the mixed alcohol extract of mulberry bark, hollyhock, and ginkgo bark. [Table 3]

[0062] Essences prepared within the above ranges or with the ingredient contents of the essence of Example 2 exhibit excellent triple whitening effects: antioxidant, anti-inflammatory, and tyrosinase activity inhibition. Regarding whitening effects, the essence of Example 2 was compared with a commercially available whitening product containing ethyl vitamin C (3-o-ethyl ascorbic acid) and salicylic acid. Volunteers were randomly divided into two groups, with a female:male ratio of 6:1 in each group, and a single-blind study was conducted. After washing their faces every morning and evening, volunteers took an appropriate amount of the test substance and applied it to their entire faces for four weeks (28 days). Volunteers underwent a VISIA skin analysis on days 0, 7, 14, and 28 of the study period. After using the same facial cleanser and makeup remover, the volunteers waited for 15 minutes in a skin testing room at a temperature of 20-25°C and a humidity level of 50±10%. Then, their skin was photographed using a 7th-generation VISIA skin testing device (manufacturer: Canfield Scientific). The lighting in the skin testing room was fully lit, and external light sources were blocked with cloth curtains. Data for superficial blemishes, intermediate blemishes (ultraviolet blemishes), and deep blemishes (brown blemishes) were analyzed using VISIA Skin Test Analysis Software version 8.5.2.

[0063] FIG. 3 shows the results of improving superficial dark spots using an essence according to an embodiment of the present disclosure and a commercially available whitening product. Bar 310 shows the test results for the essence of Example 2, and bar 320 shows the test results for the commercially available whitening product. As shown in FIG. 3, after using the commercially available whitening product for 7 days, the volunteer's superficial dark spots showed a negative improvement of -7.8%. After using the essence of Example 2 for 7 days, the volunteer's superficial dark spots showed a 7.1% improvement. After using it for 28 days, the volunteer's superficial dark spots maintained a 3.0% improvement. FIG. 4 shows the results of improving mid-layer dark spots using an essence according to an embodiment of the present disclosure and a commercially available whitening product. Bar 410 shows the test results for the essence of Example 2, and bar 420 shows the test results for the commercially available whitening product. As shown in FIG. 4, after using the commercially available whitening product for 7 days, the volunteer's mid-layer dark spots showed a negative improvement of -0.68%. After using the essence of Example 2 for 7 days, the volunteer's mid-layer dark spots showed a 4.9% improvement. After using the essence of Example 2 for 28 days, the volunteer's mid-layer dark spots maintained a 0.5% improvement. FIG. 5 shows the results of improving deep dark spots using an essence of an example of the present disclosure and a commercially available whitening product. Bar 510 shows the test results for the essence of Example 2, and bar 520 shows the test results for the commercially available whitening product. As shown in FIG. 5, after using the commercially available whitening product for 7 days, the volunteer's deep dark spots showed a negative improvement of 0.7%. After using the essence of Example 2 for 7 days, the volunteer's deep dark spots improved by 10.8%. After using the essence of Example 2 for 28 days, the volunteer's deep dark spots maintained a 7.9% improvement. As can be seen from Figures 3 to 5, apart from commercially available whitening products, the essence of Example 2 can effectively and significantly reduce melanin spots in the surface, middle and deep layers of the epidermis.

[0064] Experimental Example 6: Preparation of eye cream

[0065] Please refer to Table 4 below for the range of ingredients and content of the eye cream, and the content of each ingredient in the eye cream of Example 3. Please refer to Example 1 above for the preparation process of the mixed alcohol extract of mulberry bark, hollyhock, and ginkgo bark. [Table 4-1] [Table 4-2]

[0066] The eye cream prepared with the above content range or the component contents of the eye cream of Example 3 has excellent triple whitening effects of antioxidant, anti-inflammatory and tyrosinase activity inhibition.

[0067] Experimental Example 7: Preparation of emulsion

[0068] See Table 5 below for the ingredients and content ranges of the emulsion, and the content of each ingredient in the emulsion of Example 4. See Example 1 above for the preparation process of the mixed alcohol extract of mulberry bark, hollyhock, and ginkgo bark. [Table 5]

[0069] The emulsion prepared with the above content range or the component contents of the emulsion of Example 4 has excellent triple whitening effects of antioxidant, anti-inflammatory and tyrosinase activity inhibition.

[0070] Experimental Example 8: Preparation of mask liquid

[0071] See Table 6 below for the range of ingredients and content of the mask solution, as well as the content of each ingredient in the mask solution of Example 5. See Example 1 above for the preparation process of the mixed alcohol extract of mulberry bark, hollyhock, and ginkgo bark. [Table 6]

[0072] The mask liquid prepared with the above content range or the component contents of the mask liquid of Example 5 has excellent triple whitening effects of antioxidant, anti-inflammatory and tyrosinase activity inhibition.

[0073] Experimental Example 9: Preparation of lotion

[0074] Please refer to Table 7 below for the ingredients and content ranges of the lotion, and the content of each ingredient in the lotion of Example 6. Please refer to Example 1 above for the preparation process of the mixed alcohol extract of mulberry bark, hollyhock, and ginkgo bark. [Table 7]

[0075] The lotion prepared with the above content range or the ingredient content of the lotion of Example 6 has excellent triple whitening effects of antioxidant, anti-inflammatory and tyrosinase activity inhibition.

[0076] Based on the above, the present disclosure provides a whitening composition containing three types of plant alcohol extracts and a method for producing the same. The plant alcohol extracts include mulberry bark alcohol extract, kakidoshuu alcohol extract, and ginkgo bark alcohol extract. These plant alcohol extracts have excellent antioxidant and anti-inflammatory properties and are effective in inhibiting tyrosinase activity, so they can work together to reduce superficial, intermediate, and deep skin blemishes. The whitening composition of the present disclosure has excellent whitening effects and is gentle on the eyes and skin.

[0077] Although the present disclosure has been described in detail with reference to certain embodiments, other embodiments are possible, and therefore, the spirit and scope of the appended claims should not be limited to the description of the embodiments herein.

[0078] Obviously, those skilled in the art can make various modifications and variations to the structure of the present disclosure without departing from the scope or spirit of the disclosure. In view of the foregoing, it is intended that the present disclosure cover modifications and variations of the present disclosure that fall within the scope of the appended claims. [Explanation of symbols]

[0079] 310, 320, 410, 420, 510, 520 bar

Claims

1. A whitening composition comprising: Mulberry bark alcohol extract, Kakidoushi alcohol extract, Ginkgo tree alcohol extract, Moisturizer and Contains a solvent, The whitening composition, wherein the weight ratio of the mulberry bark alcohol extract, the kakido oshio alcohol extract, and the ginkgo bark alcohol extract is 30-95:1-20:1-20, and the total weight of the mulberry bark alcohol extract, the kakido oshio alcohol extract, and the ginkgo bark alcohol extract is 0.01 wt%-20 wt% based on 100 wt% of the total weight of the whitening composition.

2. 2. The whitening composition according to claim 1, wherein the mulberry bark alcohol extract is obtained by extracting mulberry bark with a first alcohol liquid, the kakidooshi berry alcohol extract is obtained by extracting kakidooshi berry with a second alcohol liquid, and the ginkgo bark alcohol extract is obtained by extracting ginkgo bark with a third alcohol liquid, and the first alcohol liquid, the second alcohol liquid, and the third alcohol liquid each contain an alcohol having 1 to 10 carbon atoms.

3. 3. The skin whitening composition of claim 2, wherein the first alcohol liquid, the second alcohol liquid, and the third alcohol liquid each comprise methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol, n-pentanol, 3-methylbutanol, 2-methylbutanol, 2,2-dimethylpropanol, 2-pentanol, 3-pentanol, 3-methyl-2-butanol, 2-methyl-2-butanol, hexanol, heptanol, octanol, nonanol, decanol, ethylene glycol, 1,2-propanediol, 1,4-butanediol, 1,6-hexanediol, neopentyl glycol, trimethylolpropane, glycerin, or a combination thereof.

4. 10. The skin whitening composition of claim 1, further comprising a skin conditioner, an antioxidant, a thickener, an emulsifier, an emulsion stabilizer, a fragrance, an emollient, or a combination thereof.

5. The whitening composition according to claim 1 , wherein the whitening composition is an essence, an essence cream, an eye cream, a face cream, a hand cream, a milky lotion, a mask liquid, a lotion, a sunscreen, or a barrier cream.

6. Extracting mulberry bark with a first alcoholic liquid to obtain an alcohol extract of mulberry bark; Extracting the oak leaf with a second alcoholic liquid to obtain an alcohol extract of the oak leaf; extracting the ginkgo tree with a tertiary alcohol liquid to obtain a ginkgo tree alcohol extract; The method for producing a whitening composition includes a step of mixing the mulberry bark alcohol extract, the kaki dousha alcohol extract, the ginkgo bark alcohol extract, a moisturizer, and a solvent to form a whitening composition, wherein the weight ratio of the mulberry bark alcohol extract, the kaki dousha alcohol extract, and the ginkgo bark alcohol extract is 30-95:1-20:1-20, and the total weight of the mulberry bark alcohol extract, the kaki dousha alcohol extract, and the ginkgo bark alcohol extract is 0.01 wt%-20 wt% based on 100 wt% of the total weight of the whitening composition.

7. 7. The method of claim 6, wherein the first alcohol liquid, the second alcohol liquid, and the third alcohol liquid each comprise methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, tert-butanol, n-pentanol, 3-methylbutanol, 2-methylbutanol, 2,2-dimethylpropanol, 2-pentanol, 3-pentanol, 3-methyl-2-butanol, 2-methyl-2-butanol, hexanol, heptanol, octanol, nonanol, decanol, ethylene glycol, 1,2-propanediol, 1,4-butanediol, 1,6-hexanediol, neopentyl glycol, trimethylolpropane, glycerin, or a combination thereof.

8. The first alcohol liquid contains a first alcohol having a volume percent concentration of 50% to 100%; The second alcohol liquid contains a second alcohol having a volume percent concentration of 50% to 100%; 7. The method of claim 6, wherein the tertiary alcohol liquid comprises a volume percent concentration of 50% to 100% tertiary alcohol.

9. the first alcohol liquid comprises a first alcohol and water; the second alcohol liquid comprises a second alcohol and water; The method of claim 6 , wherein the tertiary alcohol liquid comprises a tertiary alcohol and water.

10. The method according to claim 6, wherein the mulberry bark is extracted with the first alcoholic liquid, the kakidooshi with the second alcoholic liquid, and the ginkgo tree with the third alcoholic liquid, each at a temperature of 20°C to 70°C.

11. the weight ratio of the mulberry bark to the first alcohol liquid is 1:10 to 1:20; The weight ratio of the kakidooshi to the second alcohol liquid is 1:10 to 1:20, 7. The method according to claim 6, wherein the weight ratio of the marigold to the tertiary alcohol liquid is 1:10 to 1:

20.

12. The time for extracting the mulberry bark with the first alcohol liquid is 12 to 96 hours; The time for extracting the persimmon fruit with the second alcohol liquid is 12 hours to 96 hours; 7. The method of claim 6, wherein the time for extracting the marigold with the third alcoholic liquid is 12 hours to 96 hours.

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