Pre-moistened wipes with virucidal properties against non-enveloped viruses

Abstract

Disclosed is a pre-impregnated wipe product that is highly suitable for destroying non-enveloped viruses, including norovirus. The wipe composition contains a first antimicrobial agent blended with a second agent, such as a preservative. Even at a very low concentration in the wipe composition, the wipe product can completely destroy and kill non-enveloped viruses within a relatively short contact time.

Description

[Technical Field]

[0001]

[0001] This application is based on and claims priority to U.S. Provisional Patent Application No. 62 / 547,219, filed August 18, 2017, which is incorporated herein by reference. [Background technology]

[0002] Pathogenic organisms such as bacteria, fungi, and viruses continue to cause infectious diseases in humans as well as livestock and pets. Over the past few decades, disinfectant formulations have been developed to reduce or destroy pathogenic organisms and, accordingly, slow the rate of infection. Any literally hard surface can become contaminated, including floors, walls, countertops, windows, window sills, sinks, faucets, trash cans, electrical appliances, and cabinet surfaces. Disinfectants have been developed to treat hard surfaces used in hospitals, nursing homes, schools, and homes. To treat such surfaces, antimicrobial compositions for decontamination, disinfection, and / or sterilization can be added to wipes.

[0003] One particular class of antimicrobial compositions contains quaternary ammonium compounds, also known as "quats," as the microbicidal agent. The use of quats as biocides is well known. Certain amine biocides have also been used in the past.

[0004] In one application, antimicrobial compositions can be incorporated into wipe products and packaged as pre-moistened wipes. These products are generally inexpensive to produce, easy to use, and disposable after use. Pre-moistened wipes impregnated with quat-based antimicrobial compositions have been shown to provide broad-spectrum activity against many types of microorganisms, including many different bacterial strains, and have also demonstrated very fast kill times. However, quat-based and other biocide formulations incorporated into pre-moistened wipes have shown a lack of effectiveness against certain viruses in some applications.

[0005]

[0005] Viruses are classified into families based on size, symmetry, type of nucleic acid genome or deoxyribonucleic acid, and replication mode. Viruses of different families differ, for example, in their resistance to disinfectants. For example, enveloped viruses are usually more sensitive to disinfectants and therefore easier to control than non-enveloped viruses. Non-enveloped viruses have complex external structures and are more resistant to disinfectants as well as harsh environmental conditions. An example of a non-enveloped virus is norovirus. Norovirus can cause viral gastroenteritis in humans. Norovirus can affect people of all ages. Norovirus outbreaks can occur in closed or semi-closed communities, such as long-term care facilities, overnight camps, hospitals, schools, prisons, clubs, dormitories, and cruise ships, where the infection can spread very rapidly.

[0006] In view of the above, there is currently a need for disinfectant compositions that have broader spectrum antimicrobial properties, particularly against viruses such as non-enveloped viruses. There is a need for a method of enhancing the antimicrobial properties of pre-moistened wipes so that they are effective against non-enveloped viruses such as norovirus. Summary of the Invention

[0007]

[0007] Generally, the present disclosure is directed to wipe compositions having antimicrobial properties. The wipe compositions contain a blend of antimicrobial agents and / or preservatives that provide the wipe compositions with broad-spectrum activity against many different microorganisms, including viruses such as non-enveloped viruses. In addition to two or more antimicrobial agents and / or preservatives, the wipe compositions can contain various other components, such as organic solvents, alkanolamines, chelating agents, one or more surfactants, and / or liquid carriers. The wipe compositions are highly suitable for disinfecting hard surfaces. Particularly advantageously, the wipe compositions of the present disclosure not only exhibit rapid disinfection performance against many hospital-grade microorganisms, such as Staphylococcus aureus and Pseudomonas aeruginosa, but also disrupt and kill disinfectant-resistant viruses, such as norovirus.

[0008] In one embodiment, for example, the wipe composition of the present disclosure includes an aqueous solution containing a first antimicrobial agent blended with a preservative. For example, the first antimicrobial agent can include a quaternary ammonium cation or a tertiary amine. Meanwhile, the preservative can include polyhexamethylene biguanide or a salt thereof. The quaternary ammonium cation can include a halide salt of the quaternary ammonium cation. For example, the quaternary ammonium cation can include alkyldimethylbenzylammonium chloride, dialkyldimethylammonium chloride, alkyldimethylethylbenzylammonium chloride, or a mixture thereof. Alternatively, the quaternary ammonium cation can include a carbonate / bicarbonate salt of the quaternary ammonium cation.

[0009] The first antimicrobial agent and preservative may be present in the wipe composition in a concentration sufficient to completely inactivate various viruses when tested according to AOAC Test Method 961.02 and ASTM Test E2362 for presaturated paper towels after a contact time of 10 minutes or 5 minutes. For example, the completely inactivated viruses may include feline calicivirus or norovirus.

[0010] In one embodiment, the first antimicrobial agent may be present in the wipe composition in association with a preservative, i.e., a second agent, such that the weight ratio of the first antimicrobial agent to the preservative is from about 50:1 to about 1:10, e.g., from about 20:1 to about 1:8, e.g., from about 15:1 to about 1:5, e.g., from about 10:1 to about 1:3. In one embodiment, the wipe composition may comprise a concentrate intended to be diluted before use. For example, the concentrate may contain water in an amount of from about 10% to about 65% by weight. Upon dilution, the wipe composition may contain more than about 95% by weight, e.g., more than about 96% by weight, e.g., more than about 98% by weight. In one embodiment, the concentrate is formulated so that 1 part concentrate is added to about 100 parts to about 300 parts water. The wipe composition may have a pH of 8 or greater, e.g., 8.5 or greater, e.g., 9 or greater, e.g., 9.5 or greater, e.g., 10 or greater.

[0011]

[0011] Various other ingredients and components can be included in the wiping composition. For example, the wiping composition may contain a pH builder, one or more nonionic surfactants, a chelating agent, and / or an organic solvent.

[0012] In one embodiment, a surfactant is present that comprises an ethoxylated alcohol. For example, the ethoxylated alcohol is ethoxylated C 12 ~C 14 Alternatively, the surfactant may be a C alkyl alcohol having a glycoside and / or ethylene oxide unit. 12 ~C 15 It may contain a secondary alkanol, such as alkyloxypolyethyleneoxyethanol. Each surfactant may generally be present in the composition in an amount of from about 0.01% to about 2% by weight.

[0013] The wiping composition may further contain a chelating agent. The chelating agent may include, for example, tetrasodium EDTA. The chelating agent may generally be present in the composition in an amount of from about 0.01% to about 2% by weight.

[0014] The wiping composition may further contain a pH builder. The pH builder may be added, for example, to increase the pH of the composition. pH builders that can be used in accordance with the present disclosure include alkanolamines such as monoethanolamine, hydroxides such as alkali or alkaline earth metal hydroxides, carbonates, bicarbonates, borates, silicates such as metasilicates, or mixtures thereof.

[0015] In one embodiment, the present disclosure is directed to a pre-moistened wipe product containing the aforementioned wiping composition. The pre-moistened wipe product may include a liquid-absorbent substrate impregnated with the wiping composition. The liquid-absorbent substrate may include a meltblown web, a coform web, a spunbond web, an airlaid web, an airlaced web, a hydroentangled web, a bonded-carded web, or a laminate thereof.

[0016] As previously mentioned, the wiping composition incorporated into the pre-moistened wipes contains a first antimicrobial agent in combination with a second agent, such as a preservative. The first antimicrobial agent may include a quaternary ammonium cation and / or a tertiary amine and may be present in the wipe composition in an amount less than about 2 wt. %, such as less than about 1.5 wt. %, such as less than about 1 wt. %, such as less than about 0.5 wt. %, or less than about 0.4 wt. %. Indeed, in one embodiment, the first antimicrobial agent is present in the wipe composition in an amount less than 0.1 wt. %. Similarly, the second agent, i.e., a preservative, including polyhexamethylene biguanide or a salt thereof may be present in the wipe composition in an amount less than about 1.5 wt. %, such as less than about 1 wt. %, such as less than about 0.5 wt. %, such as less than about 0.3 wt. %, or less than about 0.1 wt. %. For example, polyhexamethylene biguanide or a salt thereof may be present in the wiping composition in an amount less than about 1,000 ppm, such as less than about 800 ppm, for example less than about 700 ppm, for example less than about 600 ppm, for example less than about 500 ppm. Polyhexamethylene biguanide or a salt thereof is generally present in the wiping composition in an amount greater than about 10 ppm.

[0017] In one particular embodiment, the wiping composition comprises a metasilicate and / or monoethanolamine, a chelating agent, and C 11 ~C 15 It contains a quaternary ammonium chloride in combination with a nonionic surfactant, including alkyl polyethyleneoxyethanol. In another embodiment, the wiping composition contains a quaternary ammonium carbonate / bicarbonate, a glycoside surfactant, and an organic solvent, such as isopropanol, in combination with polyhexamethylene biguanide or its salt.

[0018] The present disclosure is also directed to a method for increasing the effectiveness of pre-moistened wipes against various microorganisms, such as non-enveloped viruses. The method includes adding to the pre-moistened wipe a composition containing the aforementioned first antimicrobial agent, along with a preservative, including a biguanide compound. The first antimicrobial agent and the biguanide compound are incorporated into the pre-moistened wipe in a concentration sufficient to control and destroy non-enveloped viruses, such as norovirus, that come into contact with the wipe.

[0019]

[0019] Other features and aspects of the present disclosure are discussed in more detail below. DETAILED DESCRIPTION OF THE INVENTION

[0020]

[0020] Those skilled in the art will appreciate that this discussion is merely a description of exemplary embodiments and is not intended to limit the broader aspects of the present disclosure.

[0021] The present disclosure generally relates to an antimicrobial wiping composition that is highly suitable for wetting a wipe and using the wipe to disinfect various surfaces. The present disclosure also relates to a method for increasing the effectiveness of pre-impregnated wipes against viruses, including non-enveloped viruses. According to the present disclosure, the wiping composition contains a first antimicrobial agent blended with a preservative, i.e., a second agent, such that the antimicrobial agent acts synergistically with the preservative to destroy and kill various types of microorganisms, including viruses. Many disinfectant compositions are ineffective against, for example, non-enveloped viruses, which have complex external structures and are more resistant to harsh environmental conditions. These viruses can be particularly dangerous. For example, the non-enveloped virus norovirus can cause viral gastroenteritis in humans and, if left unchecked, can rapidly multiply, leading to outbreaks in closed or semi-closed communities, such as long-term care facilities, overnight camps, hospitals, schools, prisons, clubs, dormitories, and cruise ships. Of particular advantage, the wipe compositions of the present disclosure, which are based on a blend of antimicrobial agents and preservatives, can control and kill Norovirus.

[0021]

[0022] For example, pre-moistened wipes impregnated with the wiping compositions of the present disclosure can completely inactivate norovirus and other similar microorganisms, such as feline calicivirus, when tested according to AOAC Test Method 961.02 and ASTM Test E2362 for Pre-Impregnated Paper Towels in a contact time of 10 minutes or less, such as 8 minutes or less, for example 6 minutes or less, such as 5 minutes or less, for example 4 minutes or less, or even 3 minutes or less.

[0022]

[0023] Generally, the wipe compositions of the present disclosure contain a quaternary ammonium cation and / or a tertiary amine combined with a second agent, i.e., a preservative, which may comprise a biguanide compound.The use of two different agents not only enhances the activity spectrum of the wipe compositions against multiple microorganisms, but also makes them highly effective against many viruses, including non-enveloped viruses.Unexpectedly, it has also been discovered that the effect against non-enveloped viruses can be achieved at very low concentrations.

[0023]

[0024] In one embodiment, the first antimicrobial agent can comprise a quaternary ammonium carbonate, which can be represented by the formula:

[0024] [ka]

[0025] (In the formula, R 1 is C1~C 20 is an alkyl or aryl substituted alkyl group, R 2 is C8~C 20 is an alkyl group, preferably R 1 is R 2 is the same as R 1 is C8~C 12 alkyl group) and the composition further comprises the corresponding quaternary ammonium bicarbonate salt.

[0026] [ka]

[0027] (In the formula, R 1 C1~C may be the same or different from the above 20 is an alkyl or aryl substituted alkyl group, R 2 is the same as above or different C8~C 20 is an alkyl group, but preferably R 1 is R 2 is the same as R 1 is C8~C 12 alkyl group) In one embodiment, the first antimicrobial agent contained in the composition is a diC8-C 12 Including alkylammonium carbonates / bicarbonates. For example, in one particular embodiment, the antimicrobial or preservative composition contains didecyldimethylammonium carbonate and didecyldimethylammonium bicarbonate.

[0028] However, in other embodiments, the carbonate / bicarbonate of the quaternary ammonium cation may be selected from dioctyldimethylammonium carbonate, decyloctyldimethylammonium carbonate, benzalkonium carbonate, benzethonium carbonate, stearalkonium carbonate, cetrimonium carbonate, behentrimonium carbonate, dioctyldimethylammonium bicarbonate, decyloctyldimethylammonium bicarbonate, benzalkonium bicarbonate, benzethonium bicarbonate, stearalkonium bicarbonate, cetrimonium bicarbonate, behentrimonium bicarbonate, and mixtures of one or more such carbonates.

[0029] In another embodiment, the first antimicrobial agent can include a quaternary ammonium halide. The quaternary ammonium halide can include, for example, an alkyl quaternary ammonium halide or a benzyl ammonium halide.

[0030] Quaternary ammonium compounds, also known as "quats," typically contain at least one quaternary ammonium cation with a suitable anion. Quats generally have the general formula (1):

[0031] [ka]

[0032] The groups R1, R2, R3, and R4 can vary within wide limits, and examples of quaternary ammonium compounds with antimicrobial properties are well known to those skilled in the art. Typically, two of R1, R2, R3, and R4 are lower alkyl, meaning having 1 to 4 carbon atoms, such as methyl, ethyl, propyl, or butyl groups. Additionally, two of R1, R2, R3, and R4 are longer-chain alkyl groups or benzyl groups, having 6 to 24 carbon atoms. A - is a monovalent anion or one equivalent of a polyvalent anion of an inorganic or organic acid. - Suitable anions for are in principle all inorganic or organic anions, in particular halides, such as chloride or bromide, carboxylates, sulfonates, phosphates or mixtures thereof.

[0033] In one embodiment, the quaternary ammonium compound may have the following R groups: R is benzyl or C 6~18 -alkyl and R2 is C 1~18 -Alkyl or -[(CH2)2-O] n R5 (wherein n=1 to 20), and R3 and R4 are each independently C 1~4 -alkyl, R5 is hydrogen or unsubstituted or substituted phenyl, A - is a monovalent anion or one equivalent polyvalent anion of an inorganic or organic acid.

[0034] In one embodiment, the quaternary ammonium compound may include a dialkylammonium compound, such as a dimethyldialkylammonium compound, which may have from about 8 to about 12 carbon atoms, such as from about 8 to about 10 carbon atoms in each of the alkyl groups.

[0035] Examples of dimethyldialkylammonium compounds that can be used as the first antimicrobial agent include dimethyldioctylammonium compounds such as dimethyldioctylammonium chloride, dimethyldidecylammonium compounds such as dimethyldidecylammonium chloride, etc. Mixtures of dimethyldialkylammonium compounds can also be used, and other anions such as those mentioned above can also be used.

[0036] In another embodiment, the first antimicrobial agent may comprise a benzyl ammonium compound, such as an alkyl dimethyl benzyl ammonium compound. Generally, the alkyl group may contain from about 10 to about 18 carbon atoms, such as from about 12 to about 16 carbon atoms.

[0037] Examples of alkyldimethylbenzylammonium compounds that can be used as the first antimicrobial agent include C 12 Alkyldimethylbenzylammonium chloride, C 14 Alkyldimethylbenzylammonium chloride, and C 16 Alkyl dimethyl benzyl ammonium chloride may also be used. In addition, a mixture of these alkyl dimethyl benzyl ammonium compounds may be used. For example, the alkyl dimethyl benzyl ammonium compound may be C 12 , C 14 , and C 16 The alkyl dimethyl benzyl ammonium chloride blends are generally of the alkyl dimethyl benzyl ammonium chloride type. 16 Higher concentrations of C than alkyl constituents 12 Alkyl and C14 It is preferred that they contain alkyl moieties. Note that other anions, including those mentioned above, can also be used.

[0038] In yet another embodiment, the first antimicrobial agent may include a quaternary ammonium propionate. The quaternary ammonium propionate may include, for example, a poly(oxyalkyl)ammonium propionate. In a particular embodiment, for example, the first antimicrobial agent may include N,N-didecyl-N-methyl-poly(oxyethyl)ammonium propionate.

[0039] In addition to the quaternary ammonium cation, the first antimicrobial agent may include an amine, which may be present alone or in combination with the quaternary ammonium cation.

[0040] Suitable amines include, but are not limited to, tertiary amines such as (C6-C16) alkylamines. The term "(C6-C16) alkylamine" encompasses all amines containing a (C6-C16) alkyl group. One (C6-C16) alkylamine is N,N-bis(3-aminopropyl)dodecylamine, available from Lonza, Inc. as Lonzabac® 12.30 and 12.100. The chemical structure of N,N-bis(3-aminopropyl)dodecylamine is as follows:

[0041] [ka]

[0042]

[0038] Other representative tertiary amines include, for example, N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, N-(3-aminopropyl)-N-decyl-1,3-propanediamine, N-(3-aminopropyl)-N-tetradecyl-1,3-propanediamine, and their acid addition compounds. Other similar tertiary amines can also be used.

[0043] In accordance with the present disclosure, the above-described first antimicrobial agent is combined with at least a second agent, such as a preservative. The second agent is selected to act synergistically with the first antimicrobial agent to destroy and / or inhibit the growth of one or more target microorganisms, including non-enveloped viruses such as norovirus. Thus, the second agent, i.e., the preservative, acts as an adjuvant for the first antimicrobial agent.

[0044] In one embodiment, the preservative may comprise a guanidine, particularly a biguanide, and / or its substitution products, salts, analogs, derivatives, and / or combinations thereof. Biguanides are commonly represented by the formula:

[0045] [ka]

[0046] In the formula, R 1 , R 2 , R 3 , and R 4 are each independently selected from hydrogen, optionally substituted alkyl, optionally substituted phenyl, ethylene glycol, diethylene glycol, methylene glycol, and tetraethylene glycol, or R 1 , R 2 , R 3 , and R 4 One of them is

[0047] [ka]

[0048] wherein R 5 , R 6 , and R 7 are each independently selected from hydrogen, optionally substituted alkyl, optionally substituted phenyl, ethylene glycol, diethylene glycol, methylene glycol, and tetraethylene glycol. Substituents for the alkyl and phenyl groups include, but are not limited to, halo (e.g., chloro, bromo, fluoro, or iodo), hydroxy, and amino. The alkyl group may have 1 to 6 carbons and may be saturated or unsaturated, straight-chained, or branched.

[0049] In one embodiment, the preservative may comprise a polymeric biguanide, otherwise known as polybiguanide, or a salt, analog, or derivative thereof. In one embodiment, the polybiguanide may be a copolymer or heteropolymer. The polybiguanide may be linear, branched, cyclic, and / or dendritic. The number of polymer repeat units may vary from 2 to 1,000, e.g., 5 to 750, e.g., 10 to 500, e.g., 25 to 250, e.g., 50 to 100 repeat units. In a particular embodiment, the polybiguanide may include polyhexamethylene biguanide (PHMB), polyhexamethylene monoguanide (PHMG), polyethylene biguanide (PEB), polytetramethylene biguanide (PTMB), polyethylenehexamethylene biguanide (PHMB), polymethylene biguanides (PMBs), poly(allylbiguanidnio-co-allylamine), poly(N-vinyl-biguanide), polyallyl biguanide, and the like.

[0050] For example, in one particular embodiment, the preservative may include a polyalkylene biguanide, such as polyhexamethylene biguanide, hi one embodiment, the preservative may include polyhexamethylene biguanide hydrochloride (PHMB), also known as polyaminopropyl biguanide (PABP).

[0051] PHMB is commonly represented by the following formula but is known to exist as a complex mixture of polymeric biguanides with a variety of terminal groups, including guanidine (not shown).

[0052] [ka]

[0053] The n value represents the number of repeating units in the biguanide polymer. More specifically, PHMB may be a mixture of various biguanide polymers that may contain various combinations of end groups, such as amine, cyanoguanidino, and guanidine. Based on these three end groups alone, there may be at least six possible biguanide polymers. There may be one biguanide polymer with two terminal amine groups, designated PHMB-AA, one with two terminal cyanoguanidino groups, designated PHMB-CGCG, and one with two terminal guanidine groups, designated PHMB-GG (see below). There may also be three biguanide polymers with combinations of two different end groups. Furthermore, based on the above end groups, these include amine-cyanoguanidino (PHMB-ACG), amine-guanidino (PHMB-AG), and guanidine-cyanoguanidino (GCG). Thus, a sample of PHMB may contain a mixture of polymeric biguanides with the three mentioned end groups. Additionally, some of the compositions may include in-chain polymeric guanides (not shown). The subscript "n" represents the average number of repeating groups, and a distribution of polymer lengths exists for each of the polymers shown below.

[0054] [ka]

[0055] In the formula, n can be from about 1 to about 50, for example, from about 1 to about 20. Polyhexamethylene biguanides, such as polyhexamethylene biguanide hydrochloride, have a broad antimicrobial spectrum and are fast acting. Furthermore, the antimicrobial agents are stable over a wide pH range.

[0056] In one embodiment, the preservative may comprise a bisbiguanide, which is commonly represented by the formula below, although other forms are known to exist:

[0057] [ka]

[0058] (Wherein A and A 1 each represents either (1) an alkyl or alkoxy group containing 1 to about 4 carbon atoms, a nitro group, or a phenyl group optionally substituted with a halogen atom; (2) an alkyl group containing 1 to about 12 carbon atoms; or (3) an alicyclic group containing 4 to about 12 carbon atoms; X and X 1 each represents an alkylene group containing 1 to 3 carbon atoms; Z and Z 1 may each be 0 or 1; R and R 1 each represents either hydrogen, an alkyl group containing 1 to about 12 carbon atoms, or an aralkyl group containing 7 to about 12 carbon atoms; n is an integer from 2 to 12; (CH2) n The chain may optionally be interrupted with oxygen or sulfur atoms, aromatic nuclei, etc., or substituted with halide, hydroxyl, alkyl, alkenyl, alkynyl, or acetyl groups, aromatic nuclei, etc. In one embodiment, (CH) nThe chain may optionally be replaced by a divalent bridging group, where the divalent bridging group may be selected from, but is not limited to, alkylene, alicyclic group, cyclic nucleus, aromatic nucleus, etc., and may be substituted or interrupted by oxygen or sulfur atoms, aromatic nucleus, etc. Representative bisbiguanide compounds include, but are not limited to, chlorhexidine, alexidine, trifluoromethylphenyl bisbiguanide, analogs, derivatives, and / or salts thereof.

[0059]

[0047] When the wiping composition is in a ready-to-use form and is impregnated onto a wiper, the first antimicrobial agent and preservative can be included in the wiping composition in relatively small amounts and still be effective against a wide range of microorganisms, including non-enveloped viruses. For example, one or more quaternary ammonium compounds and / or amine compounds can be present in the composition in an amount less than about 2 wt%, such as less than about 1.5 wt%, such as less than about 1 wt%, such as less than about 0.6 wt%, such as less than about 0.4 wt%, such as less than about 0.3 wt%, such as less than about 0.2 wt%. In one embodiment, for example, the quaternary ammonium compounds and / or amine compounds can be present in the wiping composition in an amount less than 0.1 wt%, such as less than about 0.8 wt%, such as less than about 0.6 wt%. For example, the one or more quaternary ammonium compounds can be present in the composition in an amount greater than about 0.001 wt%.

[0060] Similarly, the preservative or biguanide compound may generally be present in the wiping composition in an amount less than about 2 wt. %, for example, less than about 1.8 wt. %, for example, less than about 1.6 wt. %, for example, less than about 1.4 wt. %, for example, less than about 1.2 wt. %, for example, less than about 1 wt. %, for example, less than about 0.8 wt. %, for example, less than about 0.6 wt. %, for example, less than about 0.4 wt. %, for example, less than about 0.2 wt. %, or even less than 0.1 wt. For example, one or more biguanide compounds may be present in the wiping composition in an amount less than about 0.08 wt. %, for example, less than about 0.06 wt. %. For example, one or more biguanide compounds may be present in the composition at a concentration of less than about 800 ppm, for example, less than about 600 ppm, for example, less than about 500 ppm, for example, less than about 400 ppm. Generally, the polybiguanide compound is present in the composition in an amount greater than about 10 ppm, for example, greater than about 0.001% by weight.

[0061] The first antimicrobial agent and preservative may be present in the wipe composition in a variety of different weight ratios. In one embodiment, the first antimicrobial agent may be present in an amount greater than the second agent, i.e., the preservative. Alternatively, the preservative may be present in an amount greater than the first antimicrobial agent. For example, the weight ratio of the first antimicrobial agent to the second agent in the wipe composition may generally be from about 50:1 to about 1:10, e.g., from about 20:1 to about 1:8, e.g., from about 15:1 to about 1:5, e.g., from about 10:1 to about 1:3.

[0062] In addition to the above components, the wiping composition can also contain a liquid carrier. The liquid carrier can include, for example, a polar solvent such as water. The liquid carrier is generally present in the composition in an amount greater than about 20% by weight, for example, greater than about 30% by weight, for example, greater than about 40% by weight, for example, greater than about 50% by weight, for example, greater than about 60% by weight, for example, greater than about 70% by weight, for example, greater than about 80% by weight, for example, greater than about 90% by weight, for example, greater than about 95% by weight, for example, greater than about 96% by weight, for example, greater than about 97% by weight, for example, greater than about 98% by weight, for example, greater than about 99% by weight. Generally, the liquid carrier is present in an amount less than about 99.9% by weight.

[0063] In addition to the liquid carrier, the wiping composition can also optionally contain one or more organic solvents. The organic solvent can include, for example, an alcohol. Suitable alcohols include, for example, ethanol, propanol, benzyl alcohol, phenoxyethanol, isopropanol, and mixtures thereof. Alternatively, the organic solvent can include a glycol ether. Examples of glycol ethers include diethylene glycol propyl ether, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, ethylene glycol monoisopropyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monobutyl ether, diethylene glycol monoethyl ether, diethylene glycol n-butyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol dibutyl ether, propylene glycol n-butyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether, dipropylene glycol methyl ether, dipropylene glycol butyl ether, dipropylene glycol n-propyl ether, ethylene glycol hexyl ether, and combinations thereof. In one embodiment, for example, the organic solvent present in the composition comprises dipropylene glycol n-propyl ether, ethylene glycol phenyl ether, or a mixture thereof.

[0064] When present in the composition, one or more organic solvents may generally be present in an amount greater than about 0.1% by weight, e.g., greater than about 0.2% by weight. The one or more organic solvents are generally present in an amount less than about 25% by weight, e.g., less than about 20% by weight, e.g., less than about 15% by weight. In one embodiment, a relatively small amount of organic solvent is present in the wiping composition. However, in some embodiments, it is believed that even the presence of a relatively small amount of solvent can improve the effectiveness of the composition against various microorganisms, including viruses. For example, in one embodiment, the organic solvent is present in an amount of about 0.01% to about 2% by weight, e.g., about 0.05% to about 0.8% by weight.

[0065] In one embodiment, the wiping composition can also contain a pH builder. For example, a pH builder can be added to the composition to increase the pH. In one embodiment, the pH builder can include an alkanolamine. Specific alkanolamines that can be used as pH builders include monoethanolamine, 1-amino-2-propanol, 3-amino-1-propanol, 2-(methylamino)ethanol, 2-(ethylamino)ethanol, 2-(propylamino)ethanol, 2-(isopropylamino)ethanol, diethanolamine, triethanolamine, diisopropanolamine, triisopropanolamine, or mixtures thereof. Other examples of pH builders include hydroxides, such as alkali and alkaline earth metal hydroxides, carbonates, silicates, bicarbonates, and mixtures thereof. For example, the pH builder can include sodium metasilicate, such as sodium metasilicate pentahydrate. In one embodiment, multiple pH builders can be present in the wiping composition. For example, the wiping composition can contain both a silicate and an alkanolamine.

[0066] When present, the one or more pH builders may be included in the composition in an amount greater than about 0.001% by weight, such as greater than about 0.01%, for example, greater than about 0.02% by weight, such as greater than about 0.1% by weight, for example, greater than about 0.2% by weight, for example, greater than 0.3% by weight. The one or more pH builders may generally be present in an amount less than about 5% by weight, such as less than 3% by weight, for example, less than about 2% by weight, for example, less than about 1% by weight.

[0067] The wiping compositions of the present disclosure may generally have a basic pH. For example, the pH may generally be greater than about 8, such as greater than about 8.5, such as greater than about 9, such as greater than about 9.5, such as greater than about 10, such as greater than about 10.5. The pH of the wiping composition is generally less than about 14, such as less than about 13.5, such as less than about 13.

[0068] In one embodiment, the wiping composition of the present disclosure can also contain a surfactant. Any suitable surfactant, including nonionic surfactants and / or cationic surfactants, may be added to the composition. The surfactant may generally be present in the wiping composition in an amount greater than about 0.05% by weight, for example, greater than about 0.08% by weight. The surfactant is generally present in an amount less than about 20% by weight, for example, less than about 15% by weight, for example, less than about 10% by weight, for example, less than about 5% by weight, for example, less than about 3% by weight, for example, less than about 2% by weight.

[0069] In one embodiment, one or more nonionic surfactants may be present in the wiping composition. Nonionic surfactants may, in one embodiment, include compounds formed by condensation of a hydrophilic alkylene oxide group with an aliphatic or alkylaromatic hydrophobic compound. Examples of classes of nonionic surfactants are as follows: Long chain tertiary amine oxides corresponding to the general formula: R1R2R3N→O wherein R1 contains an alkyl, alkenyl, or monohydroxyalkyl group of about 8 to about 18 carbon atoms, up to about 10 ethylene oxide moieties, and up to one glyceryl moiety; and R2 and R3 contain 1 to about 3 carbon atoms and up to about one hydroxy group, e.g., a methyl, ethyl, propyl, hydroxyethyl, or hydroxypropyl group. Examples of amine oxides suitable for use in the present invention include dimethyldodecylamine oxide, oleyldi(2-hydroxyethyl)amine oxide, dimethyloctylamine oxide, dimethyldecylamine oxide, dimethyltetradecylamine oxide, di(2-hydroxyethyl)tetradecylamine oxide, 3-dodecaoxy-2-hydroxypropyldi(3-hydroxypropyl)amine oxide, and dimethylhexadecylamine oxide.

[0070] Polyethylene oxide condensates of alkylphenols, for example, the condensation products of alkylphenols having alkyl groups containing from about 6 to 12 carbon atoms in either a linear or branched configuration with ethylene oxide present in an amount equivalent to 6 to 60 moles of ethylene oxide per mole of alkylphenol. The alkyl substituents in such compounds can be derived from the polymerization of propylene, diisobutylene, octane, or nonane.

[0071]

[0059] Those derived from the condensation of ethylene oxide with the product resulting from the reaction of propylene oxide and ethylenediamine products, and whose composition can be varied depending on the desired balance between hydrophobic and hydrophilic components, are suitable. For example, compounds containing about 40 to about 80% by weight of polyoxyethylene and having a molecular weight of about 5,000 to about 11,000, resulting from the reaction of ethylene oxide groups with the reaction product of ethylenediamine and excess propylene oxide, and a hydrophobic base having a molecular weight of about 2,500 to 3,000, are satisfactory.

[0072]

[0060] Condensation products of straight or branched chain aliphatic alcohols having 8 to 18 carbon atoms with ethylene oxide, for example, coconut alcohol ethylene oxide condensates having 10 to 30 moles of ethylene oxide per mole of coconut alcohol, the coconut alcohol portion having 10 to 14 carbon atoms.

[0073] Specific surfactants that can be used in the composition are nonylphenol ethoxylates (6-12 moles), primary alcohol ethoxylates (3-12 moles), and secondary alcohol ethoxylates (3-12 moles).

[0074] The nonionic surfactants described herein are selected to adsorb or otherwise bind to the fibers of the dry substrate of the cleaning wipe, thereby preventing the active disinfectant from adsorbing to the fibers of the dry substrate. Without being bound by theory, it is believed that the nonionic surfactants described herein alter the relative equilibrium at the surface of the cleaning wipe by modifying the surface to be less hydrophobic and the wiping composition to be less hydrophilic. For example, in the case of a quat solution, such as a dialkyl or alkylbenzyl quat solution, the net result is that the hydrophobic wiper surface is less attractive to the hydrophobic hydrocarbon tail of the quat solution. The hydrophobic group has an affinity for the substrate fiber surface. Unlike anionic surfactants, the nonionic nature of the surfactants does not attract cationic quat or chlorine-based disinfectant solutions, preventing the active disinfectant from binding to the substrate fibers.

[0075] Representative commercially available nonionic surfactants are C11-C15 secondary alkanols (alkyloxypolyethyleneoxyethanols) condensed with 7, 9, 12, 20, or 40 moles of ethylene oxide, manufactured by Dow Chemical under the tradenames Tergitol® 15-S-7, 15-S-9, 15-S-12, 15-S-20, and 15-S-40. Another representative nonionic surfactant of the same type is sold by Dow Chemical under the tradenames Tergitol® TMN-6 and TMN-10, which is believed to comprise the reaction product of trimethylnonanol and ethylene oxide. Another representative nonionic surfactant is sold by BASF under the tradename Irgasurf® HL560. Still other nonionic surfactants include block copolymers of polyoxyethylene and polyoxypropylene, which are sold by BASF under the tradename Pluronic®. A single member of any of the foregoing nonionic surfactant compositions can be used in the wiping composition, or mixtures of such representative nonionic surfactant materials can be used.

[0076] Another nonionic surfactant that may be present in the wiping composition is a glycoside. For example, the glycoside may include decyl octyl glycoside of a D-glucopyranose oligomer.

[0077] The composition may also contain a chelating agent. Suitable chelating agents include, but are not limited to, ethylenediaminetetraacetic acid (EDTA) and its salts (e.g., tetrasodium EDTA available as Versene 100® from Dow Europe SA, Horgen, Switzerland), diammonium ethylenediaminetetraacetate, aminocarboxylic acids, aminophosphonic acids, fatty acid salts, mixtures thereof, and the like. Aminophosphonic acids include ethylenediaminetetramethylenephosphonic acid, diethylenetriaminepenta(methylenephosphonic acid), ethylenediaminetri(methylenephosphonic acid), and hexamethylenediaminetetra(methylenephosphonic acid). Generally, preferred chelating agents include methylglycine diacetate (MGDA), glutamic acid, N,N-diacetic acid (GLDA), iminodisuccinic acid (IDS); ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), diethylenethamine pentamethylenephosphonic acid (DETPMP), hydroxyethyliminodiacetic acid (HEIDA), nitrilothacetic acid (NTA), aspartic acid diethoxysuccinic acid (AES), aspartic acid-N,N-diacetic acid (ASDA), diethylenethaminepentamethylenephosphonic acid (DTPMPA), hydroxyethylenediaminetetraacetic acid (HEDTA), hydroxyethylethylenediaminetriacetic acid (HEEDTA), iminodifumaric acid (IDF), iminoditartaric acid (iminoditartaric acid). acid) (IDT), iminodimaleic acid (IDMAL), iminodimalic acid (IDM), ethylenediamine difumaric acid (EDDF), ethylenediamine dimalic acid (EDDM), ethylenediamine ditartaric acid (EDDT), ethylenediamine dimaleic acid (EDDMAL), and aminotri(methylenephosphonic acid) (ATMP).More preferably, the chelating agent is selected from iminodisuccinic acid (IDS), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), hydroxyethylenediaminetetraacetic acid (HEDTA), hydroxyethylethylenediaminetriacetic acid (HEEDTA), iminodifumaric acid (IDF), iminoditataric acid (IDT), iminodimaleic acid (IDMAL), iminodimalic acid (IDM), ethylenediaminedifumaric acid (EDDF), ethylenediaminedimalic acid (EDDM), ethylenediamineditataric acid (EDDT), ethylenediaminedimaleic acid (EDDMAL), and aminotri(methylenephosphonic acid) (ATMP).

[0078] The ability of chelating agents to remove metal ions is enhanced by preventing the precipitation of hardness (calcium) from the solution. Chelating agents may also function to bind other metal ions that may adversely affect the effectiveness of the antiseptic components in the composition.

[0079] The chelating agent is preferably present in the composition in an amount of from about 0.01% to about 10%, for example from about 0.02% to about 1%, by weight of the composition.

[0068] The wiping compositions of the present disclosure may further optionally contain corrosion inhibitors, complexing agents, adjuvants, preservatives, fragrances, colorants, and the like. Representative corrosion inhibitors include, for example, organophosphorus compounds and blends of organophosphorus compounds with polymeric components. Representative adjuvants include, for example, polyethylene glycol or other similar compounds. Colorants and fragrances can be added, provided they do not interfere with the function of the composition and can aid in the identification of the composition. Generally, optional additional components comprise less than about 20% by weight of the composition. For example, each of the above-identified ingredients or components may be present in the composition in an amount generally between about 0.01% and about 5% by weight. For example, each of the above-identified ingredients may be present in the composition in an amount between about 0.1% and about 2% by weight, e.g., between about 0.3% and about 1% by weight.

[0080] The wiping compositions of the present disclosure can be supplied as concentrates and diluted before use. Alternatively, the disinfecting compositions can be provided as ready-to-use compositions.

[0081] In one embodiment, when formulated as a concentrate, the wipe composition can contain a first antimicrobial agent, which can generally comprise a quaternary ammonium cation in an amount of about 1% to about 50% by weight, e.g., about 1% to about 40% by weight, e.g., about 1% to about 25% by weight. Meanwhile, a preservative, which can comprise a biguanide compound, can be present in the concentrate in an amount of about 0.5% to about 10% by weight, e.g., about 0.5% to about 5% by weight, e.g., about 0.5% to about 3% by weight. In one embodiment, the concentrate can also contain one or more pH builders. The pH builder can be present in an amount of about 0.5% to about 40% by weight. In one embodiment, for example, a silicate can be present in an amount of about 0.5% to about 5% by weight. Alternatively, the alkanolamine alone or in combination with the silicate may be present in the concentrate in an amount of from about 5% to about 40% by weight, such as from about 10% to about 35% by weight, for example, from about 15% to about 30% by weight.

[0082] Concentrates formulated according to the present disclosure may also contain one or more surfactants. For example, one or more surfactants may be present in the concentrate in an amount of about 2% to about 15% by weight, e.g., about 2% to about 10% by weight. Additionally, one or more chelating agents may be present in an amount of about 1% to about 10% by weight, e.g., about 1% to about 8% by weight, e.g., about 1% to about 4% by weight. Optionally, the concentrate may also contain an organic solvent in an amount of about 5% to about 40% by weight, e.g., about 10% to about 35% by weight, e.g., about 15% to about 25% by weight. The remainder of the concentrate may comprise water. Water may generally be present in the concentrate in an amount of about 10% to about 80% by weight, e.g., about 30% to about 70% by weight.

[0083] In one embodiment, concentrates made in accordance with the present disclosure can be wiped with water before being applied to a wiping substrate. For example, the concentrates may be formulated so that 1 part concentrate can be mixed with about 100 parts to about 300 parts water. For example, in one embodiment, 1 part concentrate can be mixed with 128 parts water. In another embodiment, the concentrates may be formulated so that 1 part concentrate can be diluted with 256 parts water.

[0084] A wide variety of microorganisms can be killed or controlled in accordance with the present disclosure. For example, the wipe compositions of the present disclosure can control gram-positive bacteria, gram-negative bacteria, and the like. In addition to bacteria, the antimicrobial compositions of the present disclosure can also kill or prevent the growth of a variety of other microorganisms, such as fungi, spores, yeast, and mycobacteria. Of particular advantage, the wipe compositions of the present disclosure are particularly well suited to destroying viruses, even non-enveloped viruses such as norovirus.

[0085]

[0074] When used as a hard surface cleaner, the wiping composition can be delivered to the surface to be cleaned, sanitized, or disinfected by conventional means, such as pouring the composition onto the surface; spraying; which is applied to the surface via spray means including, but not limited to, pump spray applicators, pressurized spray applicators, and the like; saturated wipes; rags and buckets; mops and buckets; sponges and buckets; or via automated cleaning equipment and other similar and conventional methods that apply the antimicrobial or disinfectant composition to a surface for the purpose of sanitizing or disinfecting the surface.

[0086] To use the wiping compositions of the present disclosure, a surface is treated with a substrate by spraying, pouring, wiping, or otherwise applying the wiping composition to the surface. After being applied to the surface, the wiping composition is allowed to remain on the surface for a period of time. The wiping composition is applied to the surface and allowed to dry.

[0087] Surfaces that can be disinfected with the composition include, but are not limited to, those installed in dairy farms, homes, healthcare facilities, canneries, food processing plants, restaurants, hospitals, institutions, and industries. Any suitable hard surface, especially one that is frequently touched, can be treated according to the present disclosure. Hard surfaces can be made of, for example, glass, metals such as aluminum or stainless steel, ceramics, stone such as granite or marble, plastic or polymer materials, and the like. Specific areas of application include hard surfaces in the home, such as kitchen countertops, cabinets, appliances, trash cans, laundry areas, trash buckets, bathroom fixtures, toilets, cisterns, faucets, mirrors, vanities, bathtubs, and showers. The composition can also be used to disinfect floors, walls, furniture, mirrors, bathroom fixtures, windows, and wood surfaces, such as fence rails, porch rails, decks, roofing, siding, window frames, and door frames. Areas in a hospital include beds, gurneys, tables, canisters, toilets, trash cans, stands, cabinets, shower stalls, floors, doorknobs, bed rails, walls, or any other non-porous surface.

[0088] One particularly useful application method is to impregnate the wipe substrate with the wiping composition. In this embodiment, the wipe is a disposable wipe impregnated with the wiping composition and stored in a container from which the wipe is dispensed to the user. The container containing the wipes may contain a single wipe or multiple wipes. Suitable containers include a user-tearable pouch containing a single wipe, such as a moist paper towel, or a pouch containing several wipes in a stack, roll, or other suitable configuration with a resealable opening that allows the wipes to be removed one by one through the opening. Pouches are generally made from a fluid-impermeable material such as film, coated paper, or foil, or other similar fluid-impermeable material. Another method of dispensing the wipes of the present disclosure is to place them in a fluid-impermeable container with an opening for removing the wipes from the container. The container may be a molded plastic container with a fluid-impermeable lid. Typically, the lid has an opening for removing the wipes in the container. The wipes in the container may be stacked alternately such that when a wipe is removed from the container, the next wipe is placed in the opening of the container, ready for removal by the user. Alternatively, the wipes may be a continuous material with perforations between individual wipes in the continuous material. A continuous wipe material with perforations may be in a folded or roll form. Typically, in a roll form, the wipe material is dispensed from the center of the rolled material. In the case of an alternating stack, when a wipe is removed from the container, the next wipe is placed in the opening to facilitate removal of the next wipe when needed.

[0089] Disposable wipes offer advantages over other application media, such as reusable sponges or wipes. Unlike sponges, wipes, etc., which are used repeatedly, impregnated wipes are used only once and then discarded. Reused sponges or wipes are problematic because the sponges or wipes may harbor microorganisms that are not easily killed by the disinfecting composition.

[0090] The wiping composition can be impregnated into the wipe such that the wipe is pre-moistened and dispenses or releases the wiping composition onto the surface as the wipe is moved across the surface to be treated. Generally, the wiping composition is impregnated into the wipe such that the wiping action of the wipe dispenses the wiping composition onto the surface. Generally, the wiping composition is used in an amount of about 2 to 10 parts by weight per part by weight of wiper substrate, more preferably about 3 to about 5 parts by weight per part by weight of wiper substrate. Within these ranges, complete penetration of the substrate can be achieved. It should be noted that the amount of wiping solution may be increased or decreased to achieve complete penetration of the wipe substrate, depending on the particular wipe substrate.

[0091]

[0080] Suitable wipe substrates include woven and nonwoven materials. Essentially any nonwoven web material can be used. Exemplary nonwoven materials include, but are not limited to, meltblown, coform, spunbond, airlaid, hydroentangled nonwovens, spunlace, bonded-carded webs, and laminates thereof. Optionally, nonwovens can be laminated with film materials as well. The fibers used to make the wipe substrate can be cellulosic fibers, thermoplastic fibers, and mixtures thereof. The fibers can also be continuous, discontinuous, staple fibers, and mixtures thereof. The basis weight of the nonwoven web is about 12 g / m 2 ~200g / m 2 , or even more.

[0092] In one embodiment, the wipe is impregnated with a liquid component containing both active and inactive ingredients within legally permitted limits, and the wiping composition dispensed by the wipe contains active ingredients within legally permitted limits. After being applied to a surface, the antimicrobial wiping composition remains on the surface for a period of time.

[0093] The following examples are intended to provide a more complete understanding of the present invention, but are not intended to limit the invention. [Example]

[0094] Example 1 A wide variety of wiping compositions were formulated and tested. In Table 1, the formulations were loaded into the wipe substrate at a load ratio of 4 parts liquid to 1 part wipe (4:1), except for Sample No. 4, which was loaded into the substrate at a load ratio of 5 parts liquid to 1 part wipe. The substrate material was a woven material made from 100% polypropylene fibers, except for Sample No. 4, where the substrate material was a 100% polyester material.

[0095] [Table 1]

[0096] The pre-impregnated wipes were then evaluated for virucidal efficacy. Specifically, the pre-impregnated wipes were tested according to the Pre-impregnated Paper Towel Test, modified from test AOAC 961.02, which is based on ASTM test E2362. The pre-impregnated wipes were tested on hard surfaces against feline calicivirus, a virus that can also serve as a surrogate for norovirus.

[0097]

[0086] Feline calicivirus, also a member of the Caliciviridae family, serves as a useful model virus for efficacy testing of norovirus because these viruses share many similar properties and feline calicivirus can be grown in cell culture. The test procedure simulated product use. This method complies with U.S. Environmental Protection Agency (EPA) requirements. According to regulatory authorities, a test sample passes the virucidal efficacy test for pre-impregnated or saturated paper towels if the challenge virus is completely inactivated at all dilutions. Cytotoxicity is evident when the titer is at least 3 Log. 10The reduction in cytotoxicity must be demonstrated above the cytotoxic level. The results of the microeffect test are shown in Table 2.

[0098] [Table 2]

[0099] As noted above, the formulations containing the first and second antimicrobial agents were effective against the test viruses, but sample No. 1 failed the test. Example 2 A wide variety of wiping compositions were formulated and tested similarly to the procedures described in Example 1. Specifically, the following formulations were tested:

[0100] [Table 3]

[0101] Pre-impregnated wipes made with the above formulations were then evaluated for virucidal efficacy. Specifically, the pre-impregnated wipes were tested according to the Pre-impregnated Paper Towel Test, AOAC 961.02, which is based on ASTM Test E2362, with modifications. The pre-impregnated wipes were tested on hard surfaces against feline calicivirus, a virus that can also serve as a surrogate for norovirus.

[0102]

[0090] The results of the micro-effect test are shown below.

[0103] [Table 4]

[0104] Aspect 1 1. A method for destroying non-enveloped viruses on a surface in proximity, comprising: 1. A method comprising the step of impregnating a liquid absorbent substrate with a wiping composition, wherein the wiping composition comprises a first antimicrobial agent, wherein the first antimicrobial agent comprises a quaternary ammonium cation or a tertiary amine, and wherein the wiping composition further comprises a polybiguanide or a salt thereof. Aspect 2 2. The method of embodiment 1, wherein the wiping composition is concentrated sufficiently to completely inactivate feline calicivirus when tested according to AOAC Test Method 961.02 and ASTM Test Method E2362 for Pre-Impregnated Paper Towels at a contact time of 10 minutes. Aspect 3 2. The method of embodiment 1, wherein the wipe composition is concentrated sufficiently to completely inactivate Norovirus when tested according to AOAC Test Method 961.02 and ASTM Test Method E2362 for Pre-Impregnated Paper Towels for a 10 minute contact time. Aspect 4 Aspect 4. The method of any one of aspects 1 to 3, wherein the quaternary ammonium cation comprises a halide salt of a quaternary ammonium cation. Aspect 5 Aspect 5. The method of any one of aspects 1 to 4, wherein the quaternary ammonium cation comprises alkyldimethylbenzyl ammonium chloride, dialkyldimethylammonium chloride, alkyldimethylethylbenzyl ammonium chloride, or a mixture thereof. Aspect 6 Aspect 6. The method of any one of aspects 1 to 5, wherein the quaternary ammonium cation comprises a carbonate / bicarbonate salt of a quaternary ammonium cation. Aspect 7 7. The method of any one of aspects 1 to 6, wherein the composition further comprises an organic solvent. Aspect 8 Aspect 8. The method of any of aspects 1 to 7, wherein the wiping composition comprises a concentrate, and further comprising the step of diluting the concentrate before impregnating the liquid-absorbent substrate with the wiping composition. Aspect 9 Aspect 9. The method of any one of aspects 1 to 8, wherein the wiping composition has a pH of 8 or greater. Aspect 10 10. The method of any one of aspects 1 to 9, wherein the first antimicrobial agent is present in a weight ratio relative to the polybiguanide or salt thereof of about 50:1 to about 1:10, such as about 20:1 to about 1:8, such as about 15:1 to about 1:5, for example about 10:1 to about 1:3. Aspect 11 Aspect 11. The method of any one of aspects 1 to 10, further comprising a pH builder, wherein the pH builder comprises monoethanolamine, diethanolamine, triethanolamine, potassium carbonate, sodium carbonate, sodium bicarbonate, sodium metasilicate, or a combination thereof. Aspect 12 a liquid-absorbent substrate; a wiping composition contained within the substrate; and A pre-moistened wipe product comprising: A pre-moistened wipe product, wherein the wiping composition comprises an aqueous solution containing a first antimicrobial agent in combination with a polybiguanide or a salt thereof, wherein the first antimicrobial agent comprises a quaternary ammonium cation or a tertiary amine. Aspect 13 Aspect 13. The pre-moistened wipes of aspect 12, wherein the first antimicrobial agent comprises a halide salt of a quaternary ammonium cation. Aspect 14 Aspect 13. The pre-moistened wipe product of aspect 12, wherein the first antimicrobial agent comprises alkyldimethylbenzyl ammonium chloride, dialkyldimethylammonium chloride, alkyldimethylethylbenzyl ammonium chloride, or a mixture thereof. Aspect 15 Aspect 13. The pre-moistened wipe product of aspect 12, wherein the first antimicrobial agent comprises a carbonate / bicarbonate salt of a quaternary ammonium cation. Aspect 16 16. The pre-moistened wipe product of any of aspects 12-15, wherein the first antimicrobial agent is present in a weight ratio to the polybiguanide or salt thereof of from about 50:1 to about 1:10, such as from about 20:1 to about 1:8, such as from about 15:1 to about 1:5, for example from about 10:1 to about 1:3. Aspect 17 Aspect 17. The pre-moistened wipes product of any of aspects 12-16, wherein the wiping composition further comprises a nonionic surfactant. Aspect 18 The nonionic surfactant is a glycoside or C containing ethylene oxide units. 11 ~C 15 18. The pre-moistened wipe product of embodiment 17, comprising an alkanol. Aspect 19 Aspect 19. The pre-moistened wipes product of any of aspects 12 to 18, wherein the wiping composition contains water in an amount greater than about 90% by weight, such as greater than about 95% by weight, for example, greater than about 96% by weight. Aspect 20 Aspect 20. The pre-moistened wipes product of any of aspects 12-19, wherein the wiping composition further comprises an organic solvent. Aspect 21 Aspect 21. The pre-moistened wipes product of any of aspects 12-20, wherein the wiping composition further comprises a chelating agent. Aspect 22 22. The pre-moistened wipes product of any of aspects 12 to 21, wherein the liquid-absorbent substrate comprises a nonwoven web. Aspect 23 22. The pre-moistened wipes product of any of claims 12 to 21, wherein the liquid-absorbent substrate comprises a meltblown web, a coform web, a spunbonded web, an airlaid web, an airlaced web, a hydroentangled web, a bonded-carded web, or a laminate thereof. Aspect 24 Aspect 13. The pre-moistened wipe product of aspect 12, wherein the first antimicrobial agent comprises the tertiary amine.

[0091] These and other modifications and variations to the present invention may be practiced by those skilled in the art without departing from the spirit and scope of the present invention, as more particularly set forth in the appended claims. It should further be understood that aspects of the various embodiments may be interchanged both in whole or in part. Furthermore, those skilled in the art should recognize that the foregoing description is illustrative only and does not limit the invention as further set forth in such appended claims.

Claims

1. 1. A method for destroying non-enveloped viruses on a surface in proximity, comprising:

1. A method comprising the step of impregnating a liquid absorbent substrate with a wiping composition, wherein the wiping composition comprises a first antimicrobial agent, the first antimicrobial agent comprising a carbonate and / or bicarbonate salt of a quaternary ammonium cation or a tertiary amine selected from bis(3-aminopropyl)dodecylamine, N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, N-(3-aminopropyl)-N-decyl-1,3-propanediamine, and N-(3-aminopropyl)-N-tetradecyl-1,3-propanediamine, and the wiping composition further comprises a polybiguanide or a salt thereof, wherein the weight ratio of the first antimicrobial agent to the polybiguanide or salt thereof is from 0.75:1 to 3.5:

1.

2. 10. The method of claim 1, wherein the first antimicrobial agent and the polybiguanide or salt thereof in the wipe composition are in concentrations sufficient to completely inactivate feline calicivirus when tested according to AOAC Test Method 961.02 and ASTM Test Method E2362 for pre-impregnated paper towels at a contact time of 10 minutes.

3. 10. The method of claim 1, wherein the first antimicrobial agent and the polybiguanide or salt thereof in the wipe composition are in concentrations sufficient to completely inactivate Norovirus when tested according to AOAC Test Method 961.02 and ASTM Test Method E2362 for pre-impregnated paper towels at a contact time of 10 minutes.

4. The method of claim 1 , wherein the composition further comprises an organic solvent.

5. 5. The method of any one of claims 1 to 4, wherein the wiping composition comprises a concentrate, and further comprising the step of diluting the concentrate before impregnating the liquid-absorbent substrate with the wiping composition.

6. The method of any one of claims 1 to 5, wherein the wiping composition has a pH of 8 or greater.

7. 7. The method of any one of claims 1 to 6, further comprising a pH builder, wherein the pH builder comprises monoethanolamine, diethanolamine, triethanolamine, potassium carbonate, sodium carbonate, sodium bicarbonate, sodium metasilicate, or a combination thereof.

8. a liquid absorbent substrate; a wiping composition contained within the substrate; and A pre-moistened wipe product comprising:

1. A pre-moistened wipe product, wherein the wipe composition comprises an aqueous solution containing a first antimicrobial agent in combination with a polybiguanide or salt thereof, the first antimicrobial agent comprising a carbonate and / or bicarbonate salt of a quaternary ammonium cation or a tertiary amine selected from bis(3-aminopropyl)dodecylamine, N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, N-(3-aminopropyl)-N-decyl-1,3-propanediamine, and N-(3-aminopropyl)-N-tetradecyl-1,3-propanediamine, and wherein the weight ratio of the first antimicrobial agent to polybiguanide or salt thereof is from 0.75:1 to 3.5:

1.

9. 10. The pre-moistened wipes product of claim 8, wherein the wiping composition further comprises a non-ionic surfactant.

10. The nonionic surfactant is a glycoside or C containing ethylene oxide units. １１ ~C １５ 10. The pre-moistened wipe product of claim 9, comprising an alkanol.

11. 11. The pre-moistened wipe product of any one of claims 8 to 10, wherein the wiping composition contains water in an amount greater than 90% by weight.

12. 12. The pre-moistened wipe product of any one of claims 8 to 11, wherein the wiping composition further comprises an organic solvent.

13. 13. The pre-moistened wipe product of any one of claims 8 to 12, wherein the wiping composition further comprises a chelating agent.

14. 14. The pre-moistened wipes product of any one of claims 8 to 13, wherein the liquid-absorbent substrate comprises a nonwoven web.

15. 14. The pre-moistened wipes product of any one of claims 8 to 13, wherein the liquid-absorbent substrate comprises a meltblown web, a coform web, a spunbond web, an airlaid web, an airlaced web, a hydroentangled web, a bonded-carded web, or a laminate thereof.

16. 9. The pre-moistened wipe product of claim 8, wherein the first antimicrobial agent comprises the tertiary amine.

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