H4 antagonist compounds

Abstract

Disclosed herein are novel compounds of formula (1): [Case 1] JPEG2023552888000100.jpg4852 and salts thereof (wherein Y, Z, R 1 , R 2 , R 3 , R 4 , R 5 and n is defined herein) and their use in the treatment, prevention, amelioration, control, or reduction of risk of disorders associated with the H4 receptor.

Description

[Technical Field]

[0001] This application relates to novel compounds and their use as histamine H4 receptor antagonists.The compounds described herein can be useful for treating or preventing diseases associated with H4 receptors.This application also relates to pharmaceutical compositions comprising these compounds, and the manufacture and use of these compounds and compositions in the prevention or treatment of such diseases associated with H4 receptors. [Background technology]

[0002] Histamine is a short-acting biogenic amine produced in mast cells, where it is stored in cytoplasmic granules and released in response to various immunological and non-immunological stimuli. Histamine release from mast cells has traditionally been associated with mild to severe signs and symptoms characteristic of hypersensitivity reactions, including erythema, urticaria, pruritus, tachycardia, hypotension, ventricular fibrillation, bronchospasm, and cardiac and respiratory arrest. Numerous additional sources have now been identified, including basophils, neurons, and cancer cells. In addition to modulating a wide range of physiological processes, histamine is involved in pathological conditions, including allergy and anaphylaxis, asthma and chronic inflammation, autoimmunity, cardiovascular, neuropsychiatric and endocrine disorders, and cancer.

[0003] Histamine exerts its diverse actions primarily through binding to four types of G protein-coupled receptors (GPCRs), designated H1-H4, which are differentially expressed in various cell types and show considerable diversity across species. H2 receptors are responsible for gastric acid secretion. H3 receptors regulate the release of histamine and other neuromodulators in the CNS, and H1 receptors are involved in wakefulness and inflammatory responses.

[0004] The high-affinity H4 receptor was identified in 2000, which exhibits constitutive activity and is expressed on the majority of cells of the immune system, including, but not limited to, mast cells, monocytes, dendritic cells, eosinophils, basophils, neutrophils, and T cells. This discovery has offered the enticing prospect of a novel drug target with therapeutic potential in acute and chronic inflammation, autoimmune diseases, host defense, and psychopathic pain.

[0005] H4R shares only 40% homology with its closest relative, H3R, and neither H2 nor H1 antagonists have been shown to inhibit histamine-induced eosinophil chemotaxis. Histamine has been shown to inhibit forskolin-induced cAMP responses in a pertussis toxin (PTx)-sensitive manner, suggesting that H4R signals through heterotrimeric Gαi / o proteins. Transient expression of H4R in heterologous cellular systems (e.g., HEK293 cells) is a widely used method to measure H4 ligand signaling and binding to generate predictions of functional potency and receptor affinity, respectively.

[0006] The discovery of H4R antagonists using these techniques and their investigation in various animal disease models, including asthma, chronic pruritus, dermatitis, rheumatoid arthritis, gastric ulcerogenesis, and colitis, has confirmed that H4R antagonism results in broad anti-inflammatory effects, demonstrating the therapeutic benefit of targeting this receptor. The first H4R antagonist has already undergone a Phase 2a clinical trial in patients with moderate to severe atopic dermatitis, further validating H4 as a druggable target in patients.

[0007] Despite the publication of several H4R ligands, there is still a need for the development of novel H4R antagonists with good drug candidate qualities. These antagonists should exhibit excellent low-nM potency and affinity with complete selectivity for H1-H3 receptors. These antagonists should not exhibit agonist activity due to the risks associated with eliciting a pro-inflammatory response, and ideally, should exhibit similar pharmacological profiles across species to support PK / PD in various animal models of disease. These antagonists should be metabolically stable, have excellent PK, be nontoxic, and demonstrate excellent H4 specificity in extensive safety panel profiling.

[0008] The human ether-a-go-go-related gene (hERG) encodes the pore-forming subunit of the rapidly activating delayed rectifier potassium channel (IKr), which plays a critical role in determining ventricular repolarization and the QT interval on the electrocardiogram (the QT interval is the time it takes for ventricular depolarization and repolarization). It is widely known that hERG is highly sensitive to inhibition by a wide range of structurally diverse compounds. Drug administration that inhibits or impairs the channel's ability to conduct electrical current across the cell membrane can result in a potentially fatal disorder called QT syndrome. Several clinically successful marketed drugs have a tendency to inhibit hERG, which carries the risk of sudden death as a side effect, making hERG inhibition an important target to be avoided during drug development.

[0009] The compounds of the present invention are antagonists of the H4 receptor. Certain compounds have low hERG inhibition, making them particularly beneficial. Summary of the Invention

[0010] The present invention provides compounds that have activity as H4 receptor antagonists, more specifically, compounds that combine H4 receptor antagonism with low hERG activity.

[0011] Thus, in one embodiment, the present invention provides a compound of formula (1):

[0012] [ka] or its salts (In the formula, Z is H, NH2 or C 1~3 is alkyl, Y is

[0013] [ka] is selected from the group consisting of n is 0 or 1, R 1 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 2 is H, optionally substituted C 1~6 Alkyl, optionally substituted C 3~6 cycloalkyl or an optionally substituted 3- to 6-membered heterocyclyl group, the optional substituents being OC 1~3 alkyl or 1 to 6 fluorine atoms, or R 2 is R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 3 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 1 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 2 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 4 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 4 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 5 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 5 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 4 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 6 is H or methyl) to provide.

[0014] Particular compounds are compounds of formula (1a) and (1b):

[0015] [ka] or salts thereof, 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0016] Particular compounds are compounds of formula (2a) and (2b):

[0017] [ka] or salts thereof, Z, R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0018] Particular compounds are compounds of formula (2c) and (2d):

[0019] [ka] or salts thereof, Z, R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0020] A particular compound is a compound of formula (2e):

[0021] [ka] or a salt thereof, 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0022] Particular compounds are compounds of formula (3a) and (3b):

[0023] [ka] or salts thereof, Z, R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0024] A particular compound is a compound of formula (3c):

[0025] [ka] or a salt thereof, 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0026] A particular compound is a compound of formula (4):

[0027] [ka] or salts thereof, 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0028] A particular compound is a compound of formula (5):

[0029] [ka] or a salt thereof, 2 , R 3 and n is as defined above.

[0030] A particular compound is a compound of formula (5a):

[0031] [ka] or a salt thereof, 2 , R 3 and n is as defined above.

[0032] Particular compounds are compounds of formula (6a) and (6b):

[0033] [ka] or salts thereof, 2 and R 3 is as defined above.

[0034] Particular compounds are compounds of formula (7a) and (7b):

[0035] [ka] or salts thereof, 2 and R 3 is as defined above.

[0036] This compound can be used as H4 receptor antagonist.This compound can be used for the manufacture of medicine.This compound or medicine can be used for treating, preventing, improving, controlling or reducing the risk of inflammatory disorders, including asthma, chronic pruritus, dermatitis, rheumatoid arthritis, gastric ulcerogenesis and colitis. DETAILED DESCRIPTION OF THE INVENTION

[0037] (Detailed Description of the Invention) The present invention relates to novel compounds.The present invention also relates to the use of novel compounds as H4 receptor antagonists.The present invention further relates to the use of novel compounds for use as H4 receptor antagonists or in the manufacture of medicaments for the treatment of dysfunction of the H4 system.The present invention further relates to compounds, compositions and medicaments that are selective H4 receptor antagonists.

[0038] The present invention further relates to compounds, compositions and medicaments useful in the treatment of acute and chronic inflammation, autoimmune diseases, impaired host defenses and neuropathic pain.

[0039] The present invention further relates to compounds, compositions and medicaments useful in the treatment of inflammatory disorders including asthma, chronic pruritus, dermatitis, rheumatoid arthritis, gastric ulcerogenesis and colitis.

[0040] The compounds of the present invention are compounds according to formula (1):

[0041] [ka] or its salts (In the formula, Z is H, NH2 or C 1~3is alkyl, Y is

[0042] [ka] is selected from the group consisting of n is 0 or 1, R 1 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 2 is H, optionally substituted C 1~6 Alkyl, optionally substituted C 3~6 cycloalkyl or an optionally substituted 3- to 6-membered heterocyclyl group, the optional substituents being OC 1~3 alkyl or 1 to 6 fluorine atoms, or R 2 is R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 3 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 1 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 2 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 4 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 4 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 5 to form a ring optionally substituted with 1 to 6 fluorine atoms, R5 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 4 to form a ring optionally substituted with 1 to 6 fluorine atoms, R 6 is H or methyl) Includes.

[0043] In the compounds herein, Z can be H. Z can be NH. Z can be C 1~3 Z can be alkyl. Z can be methyl.

[0044] In the compounds herein, Y can be an optionally substituted 3-aminopyrrolidine ring. Y can be an optionally substituted 3-aminoazetidine ring. Y can be an optionally substituted piperazine ring. Y can be an optionally substituted octahydro-1H-pyrrolo[3,4-b]pyridine ring system. Y can be 3-aminopyrrolidine. Y can be 3-aminoazetidine. Y can be piperazine. Y can be octahydro-1H-pyrrolo[3,4-b]pyridine. Y can be N-methylazetidin-3-amine. Y can be N-methylpyrrolidin-3-amine. Y can be N-methylpiperazine. Y can be (3R)-N-methylpyrrolidin-3-amine. Y can be (3R)-pyrrolidin-3-amine. Y can be (4aR,7aR)-octahydro-1H-pyrrolo[3,4-b]pyridine.

[0045] Y is

[0046] [ka] It can be said that:

[0047] Y is

[0048] [ka] It can be said that:

[0049] Y is

[0050] [ka] It can be said that:

[0051] Y is

[0052] [ka] It can be said that:

[0053] In the compounds herein, R 1 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 R can be alkyl. 1 R can be H or methyl. 1 can be H. R 1 is C 1~3 R can be alkyl. 1 is R 3 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 1 R can be methyl. 1 is R 3 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 1 is R 3 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 1 is R 3 can be attached to form a 3- to 6-membered heterocycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 1 is R 3can be joined to form a 5-membered heterocycloalkyl ring optionally substituted with 1 to 6 fluorine atoms.

[0054] In the compounds herein, R 2 is H, optionally substituted C 1~6 Alkyl, optionally substituted C 3~6 It can be a cycloalkyl or an optionally substituted 3-6 membered heterocyclyl group, the optional substituents being selected from OMe or 1-3 fluorine atoms, or R 2 is R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms. 2 R can be selected from the group consisting of H, methyl, ethyl, isopropyl, cyclopropyl, isobutyl, trifluoromethyl, CHOMe, CH(CH)OMe, C(CH)OMe, and oxetanyl. 2 can be H. R 2 R can be methyl. 2 R can be ethyl. 2 R can be isopropyl. 2 R can be cyclopropyl. 2 R can be isobutyl. 2 R can be trifluoromethyl. 2 can be CH2OMe. R 2 can be CH(CH3)OMe. R 2 can be C(CH3)2OMe. R 2 R can be oxetanyl. 2 is R 3 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 2 is R 3 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 2 is R 3can be bonded to form a four-membered ring optionally substituted with 1 to 6 fluorine atoms. 2 is R 3 can be bonded to form a 3- to 6-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 2 is R 3 can be attached to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 2 is R 3 can be joined to form a 4-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms.

[0055] In the compounds herein, R 3 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 R can be alkyl. 3 can be H. R 3 is C 1~3 R can be alkyl. 3 is R 1 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 4 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 3 R can be methyl. 3 is R 1 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 4 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 1R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be bonded to form a four-membered ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 1 can be attached to form a 3- to 6-membered heterocycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be bonded to form a 3- to 6-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 4 can be bonded to form a 3- to 6-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 1 can be attached to form a heterocycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be attached to form a cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 4 can be attached to form a cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 1 can be attached to form a 5-membered heterocycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be attached to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 2 can be attached to form a 4-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 3 is R 4can be joined to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms.

[0056] In the compounds herein, R 4 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 3 to form a ring optionally substituted with 1 to 6 fluorine atoms, or R 5 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 4 R can be selected from H, methyl, ethyl or isopropyl. 4 can be H. R 4 is C 1~3 R can be alkyl. 4 R can be methyl. 4 R can be ethyl. 4 R can be isopropyl. 4 is R 3 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 5 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 3 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 5 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 3 can be attached to form a cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 5 can be attached to form a cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 4 is R3 can be bonded to form a 3- to 6-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 5 can be bonded to form a 3- to 6-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 3 can be attached to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 4 is R 5 can be joined to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms.

[0057] In the compounds herein, R 5 is H or C optionally substituted with 1 to 6 fluorine atoms 1~3 alkyl or R 4 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 5 can be H. R 5 is C optionally substituted with 1 to 6 fluorine atoms 1~3 R can be alkyl. 5 is C 1~3 R can be alkyl. 5 is R 4 can be bonded to form a ring optionally substituted with 1 to 6 fluorine atoms. 5 R can be methyl. 5 is R 4 R can be bonded to form a 3- to 6-membered ring optionally substituted with 1 to 6 fluorine atoms. 5 is R 4 R can be bonded to form a five-membered ring optionally substituted with 1 to 6 fluorine atoms. 5 is R 4 can be attached to form a cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms. 5 is R4 can be joined to form a 5-membered cycloalkyl ring optionally substituted with 1 to 6 fluorine atoms.

[0058] In the compounds herein, n can be 0. n can be 1.

[0059] The compounds of the present invention are compounds according to formula (4):

[0060] [ka] or salts thereof, 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above.

[0061] base R 1 , R 2 , R 3 , R 4 and R 5 The part containing

[0062] [ka] It can be selected from the group consisting of:

[0063] The compound is

[0064] [ka]

[0065] [ka]

[0066] [ka]

[0067] [ka] or a salt thereof.

[0068] The compound is (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Cyclopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-Isobutyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-(methoxymethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((S)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 6-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6S,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6R,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6S,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6R,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-Isopropyl-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-6a-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (S)-6a-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-aminopyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((S)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-(2-methoxypropan-2-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Cyclopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((S)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-(3-aminoazetidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-(3-aminoazetidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine (R)-7-Isopropyl-4-(4-methylpiperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-(piperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((R)-7-cyclopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (3R)-1-(7-(methoxymethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (3R)-N-methyl-1-(6-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-(7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylazetidin-3-amine; (3R)-1-(7-isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-4-(3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazepin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-1-((S)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((R)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; 4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[(3R)-3-aminopyrrolidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[3-(methylamino)azetidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclobutane-1,7'-pyrimido[5,4-b][1,4]oxazine]-2'-amine; 7,7-dimethyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 8-Ethyl-4-[3-(methylamino)azetidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[(3R)-3-aminopyrrolidin-1-yl]-8-ethyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 8-Ethyl-4-[(4aR,7aR)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (3R)-1-(8-ethyl-8-methyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; 8-Ethyl-8-methyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclopentane-1,7'-pyrimido[5,4-b][1,4]oxazine]-2'-amine; (3R)-N-methyl-1-(6'H,8'H-spiro[cyclopentane-1,7'-pyrimido[5,4-b][1,4]oxazin]-4'-yl)pyrrolidin-3-amine; (3R)-1-(3,3-difluoro-6'H,8'H-spiro[cyclobutane-1,7'-pyrimido[5,4-b][1,4]oxazin]-4'-yl)-N-methylpyrrolidin-3-amine; 7-Ethyl-7-methyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 3,3-Difluoro-4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclobutane-1,7'-pyrimido[5,4-b][1,4]oxazine]-2'-amine; or a salt thereof.

[0069] The compound may be a salt of any of the compounds described above. The compound may be a dihydrochloride salt. The compound may be a hydrochloride salt. The compound may be a ditrifluoroacetate salt. The compound may be a trifluoroacetate salt.

[0070] The compound may be selected from the group consisting of: (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-7-Cyclopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine ditrifluoroacetate; (S)-7-((R)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (S)-7-((S)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (6S,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (6R,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (6S,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (6R,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((S)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-7-ethyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine ditrifluoroacetate; (R)-7-Cyclopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine ditrifluoroacetate; (S)-7-((R)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride; (R)-4-(3-aminoazetidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine ditrifluoroacetate; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine hydrochloride; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine hydrochloride; (R)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (S)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (R)-8-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (S)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (R)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; 7-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; 7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; 7-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (S)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (R)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (S)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (R)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepine-2-amine ditrifluoroacetate; (R)-1-((S)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepin-4-yl)-N-methylpyrrolidin-3-amine trifluoroacetate; and (R)-1-((R)-8-Isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxoazepin-4-yl)-N-methylpyrrolidin-3-amine trifluoroacetate.

[0071] Specific examples of compounds include those that have low hERG activity.

[0072] Specific examples of compounds with low hERG activity are compounds selected from the group consisting of: (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-(methoxymethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 6-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-aminopyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-Isopropyl-4-(piperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; or salts thereof.

[0073] definition In this application, the following definitions apply unless otherwise indicated.

[0074] The term "treatment," in reference to the use of any of the compounds described herein, including compounds of Formula (1), Formula (1a), Formula (1b), Formula (2a), Formula (2b), Formula (2c), Formula (2d), Formula (2e), Formula (3a), Formula (3b), Formula (3c), and Formula (4), is used to describe any form of intervention in which a compound is administered to a subject suffering from, or at risk of, or potentially at risk for the disease or disorder in question. Thus, the term "treatment" encompasses both prophylactic (preventative) treatment and treatment in which measurable or detectable symptoms of the disease or disorder are present.

[0075] The term "effective therapeutic amount" (e.g., in relation to a method for treating a disease or condition) refers to an amount of a compound that is effective to produce a desired therapeutic effect. For example, if the condition is pain, an effective therapeutic amount is an amount sufficient to achieve a desired level of pain relief. A desired level of pain relief can be, for example, complete elimination of pain or a reduction in the severity of pain.

[0076] "C 1~3 The term "alkyl" as in "alkyl", "C 3~6 "Cycloalkyl" as in "cycloalkyl," "heterocycloalkyl" as in "3- to 6-membered heterocyclyl group," and "heterocyclyl" are all used in their conventional sense (e.g., as defined in the IUPAC Gold Book) unless otherwise indicated.

[0077] To the extent that any of the compounds described herein have chiral centers, the present invention extends to all optical isomers of such compounds, whether in the form of racemates or resolved enantiomers. The invention described herein relates to all crystal forms, solvates, and hydrates of any of the disclosed compounds, prepared as such. To the extent that any of the compounds disclosed herein have acidic or basic centers, such as carboxylate or amino groups, all salt forms of said compounds are included herein. For pharmaceutical use, the salts should be considered pharmaceutically acceptable salts.

[0078] The salt or pharmaceutically acceptable salt that can be mentioned includes acid addition salt and base addition salt.Such salt can be formed by conventional means, for example, by reacting the free acid or free base form of the compound with one or more equivalents of suitable acid or base, optionally in a solvent or medium in which the salt is insoluble, and then removing the solvent or medium using standard techniques (for example, in vacuo, by lyophilization or by filtration).Salt can also be prepared by exchanging the counterion of the compound in the form of salt with another counterion, for example, by using a suitable ion exchange resin.

[0079] Examples of pharmaceutically acceptable salts include acid addition salts derived from inorganic and organic acids, and salts derived from metals such as sodium, magnesium, potassium, and calcium.

[0080] Examples of acid addition salts include acetic acid, 2,2-dichloroacetic acid, adipic acid, alginic acid, arylsulfonic acids (e.g., benzenesulfonic acid, naphthalene-2-sulfonic acid, naphthalene-1,5-disulfonic acid, and p-toluenesulfonic acid), ascorbic acid (e.g., L-ascorbic acid), L-aspartic acid, benzoic acid, 4-acetamidobenzoic acid, butanoic acid, (+)camphoric acid, camphorsulfonic acid, and the like. Sulfonic acid, (+)-(1S)-camphor-10-sulfonic acid, capric acid, caproic acid, caprylic acid, cinnamic acid, citric acid, cyclamic acid, dodecyl sulfuric acid, ethane-1,2-disulfonic acid, ethanesulfonic acid, 2-hydroxyethanesulfonic acid, formic acid, fumaric acid, galactaric acid, gentisic acid, glucoheptonic acid, gluconic acid (e.g., D-gluconic acid), glucuronic acid (e.g., D-glucuronic acid), ronic acid), glutamic acid (e.g., L-glutamic acid), α-oxoglutaric acid, glycolic acid, hippuric acid, hydrobromic acid, hydrochloric acid, hydroiodic acid, isethionic acid, lactic acid (e.g., (+)-L-lactic acid and (±)-DL-lactic acid), lactobioic acid, maleic acid, malic acid (e.g., (-)-L-malic acid), malonic acid, (±)-DL-mandelic acid, metaphosphoric acid, methanesulfonic acid, 1- Included are acid addition salts formed with hydroxy-2-naphthoic acid, nicotinic acid, nitrate, oleic acid, orotic acid, oxalic acid, palmitic acid, pamoic acid, phosphoric acid, propionic acid, L-pyroglutamic acid, salicylic acid, 4-amino-salicylic acid, sebacic acid, stearic acid, succinic acid, sulfuric acid, tannic acid, tartaric acid (e.g., (+)-L-tartaric acid), thiocyanic acid, undecylenic acid, and valeric acid.

[0081] Also included are any solvates of compounds and their salts.Preferred solvates are those formed by incorporating molecules of non-toxic, pharmaceutically acceptable solvents (hereinafter referred to as solvating solvents) into the solid-state structure (e.g., crystalline structure) of the compounds of the present invention.Examples of such solvents include water, alcohols (such as ethanol, isopropanol, and butanol) and dimethyl sulfoxide.Solvates can be prepared by recrystallizing the compounds of the present invention with a solvent or a mixture of solvents containing a solvating solvent.Whether a solvate is formed in any given example can be determined by analyzing the crystals of the compound using well-known standard techniques, such as thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and X-ray crystallography.

[0082] Solvate can be stoichiometric solvate or non-stoichiometric solvate.Particular solvate can be hydrate, and the example of hydrate includes hemihydrate, monohydrate and dihydrate.For more detailed discussion of solvate and the method used to make and identify solvate, refer to Bryn et al., Solid-State Chemistry of Drugs, Second Edition, published by SSCI, Inc. of West Lafayette, IN, USA, 1999, ISBN 0-967-06710-3.

[0083] In the context of the present invention, the term "pharmaceutical composition" refers to a composition containing an active agent and one or more pharmaceutically acceptable carriers. Depending on the mode of administration and the nature of the dosage form, the composition may further contain components selected from, for example, diluents, adjuvants, excipients, vehicles, preservatives, fillers, disintegrants, wetting agents, emulsifiers, suspending agents, sweeteners, flavorings, fragrances, antibacterial agents, antifungal agents, lubricants, and dispersing agents. The composition may take the form of, for example, tablets, dragees, powders, elixirs, syrups, liquid preparations including suspensions, sprays, inhalants, tablets, lozenges, emulsions, solutions, cachets, granules, capsules, and suppositories, as well as liquid preparations for injection, including liposome preparations.

[0084] The compounds of the present invention may contain one or more isotopic substitutions, and a reference to a particular element includes within its scope all isotopes of that element. For example, a reference to hydrogen includes within its scope: 1 H, 2 H(D) and 3 Similarly, when carbon and oxygen are mentioned, the following are included within their scope: 12 C. 13 C and 14 C and 16 O and 18 O. In a similar manner, when a particular functional group is referred to, isotopic variations are included within its scope unless the context indicates otherwise. For example, when an alkyl group such as an ethyl group or an alkoxy group such as a methoxy group is referred to, variations in which one or more hydrogen atoms in the group are in the form of a deuterium or tritium isotope are also encompassed, for example, an ethyl group in which all five hydrogen atoms are deuterium isotopes (perdeuteroethyl) or a methoxy group in which all three hydrogen atoms are deuterium isotopes (trideuteromethoxy). Isotopes may be radioactive or non-radioactive.

[0085] Therapeutic dosage can vary depending on the patient's requirements, the severity of the condition to be treated and the compound used.Determining the appropriate dosage for specific circumstances is within the skill of the art.Generally, treatment is started with a smaller dosage that is less than the optimal dose of the compound.Then, this dosage is increased by small increments until the optimal effect under the circumstances is reached.For convenience, if desired, the total daily dosage can be divided and administered in portions throughout the day.

[0086] The magnitude of an effective dose of a compound will, of course, vary depending on the nature and severity of the condition being treated, as well as the particular compound and its route of administration. Selection of an appropriate dosage is within the ability of one skilled in the art without undue burden. Generally, the daily dose may be about 10 μg to about 30 mg per kg of body weight for humans and non-human animals, preferably about 50 μg to about 30 mg per kg of body weight for humans and non-human animals, e.g., about 50 μg to about 10 mg per kg of body weight for humans and non-human animals, e.g., about 100 μg to about 30 mg per kg of body weight for humans and non-human animals, e.g., about 100 μg to about 10 mg per kg of body weight for humans and non-human animals, and most preferably about 100 μg to about 1 mg per kg of body weight for humans and non-human animals.

[0087] Method for preparing compounds of formula (1) Compounds of formula (1) can be prepared in accordance with synthetic methods well known to those skilled in the art and described herein. Thus, in one embodiment, the present invention provides a method for preparing a compound defined as formula (1) above, comprising:

[0088] (A) When it is required to prepare a compound of formula (1) wherein Z is NH2, the reaction sequence illustrated in Scheme 1 below:

[0089] [ka]

[0090] Specifically, 2,4,6-trichloropyrimidin-5-ol (10), which is readily available by dealkylation of commercially available 2,4,6-trichloro-5-methoxypyrimidine, can be converted under Mitsunobu conditions to a protected amino alcohol (R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above, and PG 1 represents a suitable amino protecting group such as Boc, Cbz, Fmoc, Teoc or Bn) to give substituted trichloropyrimidines (R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above, and PG 1 represents a suitable amino protecting group such as Boc, Cbz, Fmoc, Teoc, or Bn. Typically, the Mitsunobu reaction is carried out using a trisubstituted phosphine reagent such as Ph3P or Bu3P in the presence of an azodicarboxylate species such as DEAD, DIAD, or TMAD in a solvent such as THF, toluene, MeCN, or DCM at about 0° C. to about room temperature, or occasionally with gentle heating at about 50° C. to 100° C.

[0091] Once the substituted trichloropyrimidine of formula (12) is formed, the amino functionality is protected by a protecting group PG 1 and is deprotected using conditions related to the nature of the compound and well understood by those skilled in the art, and the resulting product is then cyclized by an intramolecular SNAr substitution reaction or by an intramolecular transition metal catalyzed coupling reaction to give a compound of formula (13) (R 1 , R 2 , R 3 , R 4 , R 5and n is as defined above. The SNAr displacement reaction can be carried out in an open vessel, or optionally in a sealed vessel, optionally at a pressure greater than atmospheric, in a suitable solvent such as 1,4-dioxane, THF, DMF, acetone, DCM, MeCN, HO, EtOH, IPA, DMSO, or NMP, or a combination of suitable solvents, at a temperature between about room temperature and about 200°C using conventional heating, or optionally by heating with microwave irradiation, with a tertiary amine base such as TEA or DIPEA, or an inorganic base such as KCO, CsCO, NaCO, or NaHCO, or KO. t The transition metal catalyzed coupling reaction is typically carried out in the presence of a strong base such as Bu, NaH, or LiHMDS. The transition metal catalyzed coupling reaction is carried out in an open or optionally closed vessel, optionally at pressures greater than atmospheric pressure, in a solvent such as 1,4-dioxane, THF, DME, t in the presence of a sub-stoichiometric amount of a transition metal catalyst such as Pd(OAc) (CAS: 3375-31-3), Pd(dba) (CAS: 51364-51-3), Pd(dppf)Cl (CAS: 72287-26-4), Pd(PPh)Cl (CAS: 13965-03-2) or Pd(PPh) (CAS: 14221-01-3), optionally PhP, BuP, in a suitable solvent such as BuOH or toluene, or a combination of suitable solvents, at a temperature between about room temperature and about 200°C by heating using conventional heating or optionally with microwave irradiation. t Bu3P, XPhos (CAS: 564483-18-7), Xantphos (CAS: 161265-03-8), t NaO in the presence of a substoichiometric amount of a phosphine ligand such as BuBrettPhos (CAS: 1160861-53-9) or BINAP (CAS: 76189-55-4, 76189-56-5) t Bu or KO t It is usually carried out in the presence of an alkoxide base such as Bu, an inorganic base such as K3PO4, K2CO3, Cs2CO3 or NaOCN, or a tertiary amine base such as TEA or DIPEA, or a suitable salt combination.

[0092] Once formed, the compound of formula (13) can be prepared by displacing the 4-chloro substituent in the compound of formula (13) to form a compound of formula (15) (R 1 , R 2 , R 3 , R 4 , R 5 , n and Y are as defined above) with an amine of formula (14) (Y is as defined above). Once formed, the 2-chloro substituent in compounds of formula (15) can be reacted with a suitably protected NH equivalent (16) (PG) using another transition metal catalyzed coupling reaction similar to that described above. 2 can be replaced by a protecting group or group such as Boc, Cbz, (Boc)2, Ac, Bz, Bn, Bn2, PMB or DMB). Finally, the protecting group or group PG 2 is the protecting group PG 2 and conditions well understood by those skilled in the art to provide the desired compound of formula (17) (R 1 , R 2 , R 3 , R 4 , R 5 , n and Y are as defined above).

[0093] It will be understood by those skilled in the art that the order of steps arranged in Scheme 1 may be completed in an order different from that shown without affecting the overall success of the synthesis of the desired compound of formula (17). For example, in Scheme 2 below, displacement of the 4-chloro substituent in the substituted trichloropyrimidine of formula (12) by reaction with an amine of formula (14) using the SNAr displacement reaction or transition metal catalyzed coupling reaction described above is carried out in Step 2 to afford a substituted aminodichloropyrimidine of formula (18) (R 1 , R 2 , R 3 , R 4 , R 5, n and Y are as defined above, and PG 1 represents a suitable amino protecting group such as Boc, Cbz, Fmoc, Teoc or Bn. Once the substituted aminodichloropyrimidine of formula (18) is formed, the amino functionality can be protected with a protecting group PG 1 and well understood by those skilled in the art, the resulting product can then be cyclized to form a compound of formula (15) by an intramolecular SNAr substitution reaction as described above, or by an intramolecular transition metal catalyzed coupling reaction. Synthesis of the desired compound of formula (17) from a compound of formula (15) then follows as described in Scheme 1.

[0094] [ka]

[0095] Alternatively, as illustrated in Scheme 3 below, displacement of the 4-chloro substituent with an amine of formula (14) can be carried out directly on 2,4,6-trichloropyrimidin-5-ol (10) in step 1 of the sequence using an SNAr displacement reaction or transition metal-catalyzed coupling reaction similar to those described above to form the 4-amino-2,6-dichloropyrimidin-5-ol analog of formula (19) (Y is as defined above). Once formed, the 4-amino-2,6-dichloropyrimidin-5-ol analog of formula (19) can then undergo reaction with a protected amino alcohol of formula (11) in step 2 using Mitsunobu conditions similar to those described above to form the substituted aminodichloropyrimidine of formula (18). Synthesis of the desired compound of formula (17) from the substituted aminodichloropyrimidine of formula (18) then follows as described in Scheme 2.

[0096] [ka]

[0097] Scheme 4 below shows yet another variation, in which a protected 2,4,6-trichloropyrimidin-5-ol (PG 3 represents a suitable phenolic OH protecting group such as Me or PMB) can be converted to an amino alcohol of formula (21) (R 1 , R 2 , R 3 , R 4 , R 5 and n is as defined above) to displace the 4-chloro substituent in the protected 2,4,6-trichloropyrimidin-5-ol of formula (20) to form a protected 4-amino-2,6-dichloropyrimidin-5-ol of formula (22). 3 and conditions well understood by those skilled in the art to react with the protecting group PG in the protected 4-amino-2,6-dichloropyrimidin-5-ol of formula (22). 3 Removal of the aryl group allows for an intramolecular Mitsunobu reaction similar to that described above to form compounds of formula (13). The synthesis of the desired compounds of formula (17) from compounds of formula (13) then follows as described in Scheme 1.

[0098] [ka]

[0099] Those skilled in the art will understand that the reaction steps illustrated in Schemes 1-4 can be combined in various ways as needed to successfully prepare the desired compound of formula (17). It will also be apparent that there may be additional steps, including functional group modification, protection, or deprotection, introduced into the overall synthetic sequence. For example, a carboxylic acid or ester group may be reduced to an alcohol, which is then protected with a silyl-based protecting group. An amide, nitrile, or nitro group may be reduced to an amine, which is then protected with a carbamate-based protecting group. Primary or secondary amines may be further substituted using alkylation reactions. A Boc protecting group may be reduced to a methyl group.

[0100] (B) When Z is H, the preparation of a compound of formula (1) is required: 4,6-dichloropyrimidin-5-ol (23) or protected 4,6-dichloropyrimidin-5-ol (PG 3 represents a suitable phenolic OH protecting group such as Me or PMB), the desired compound of formula (25) (R 1 , R 2 , R 3 , R 4 , R 5 , n and Y are as defined above) to obtain:

[0101] [ka]

[0102] Alternatively, the 2-chloro substituent in compounds of formula (15) can be removed using reductive conditions such as treatment with H gas in the presence of a transition metal catalyst such as palladium on carbon or palladium hydroxide on carbon in a solvent such as MeOH or EtOH, optionally in the presence of a tertiary amine base such as EtN or DIPEA, optionally at greater than atmospheric pressure, to provide the desired compound of formula (25). Alternatively, reductive dichlorination can be carried out using ammonium formate in the presence of a transition metal catalyst such as palladium on carbon or palladium hydroxide on carbon in a solvent such as MeOH or EtOH at a temperature between about room temperature and about the boiling point of the solvent used:

[0103] [ka]

[0104] (C) When Z is methyl, the preparation of a compound of formula (1) is required: 4,6-dichloro-2-methylpyrimidin-5-ol (26) or protected 4,6-dichloro-2-methylpyrimidin-5-ol (PG 3 represents a suitable phenolic OH protecting group such as Me or PMB), the desired compound of formula (28) (R 1 , R 2 , R 3 , R 4 , R 5 , n and Y are as defined above) to obtain:

[0105] [ka]

[0106] Alternatively, the 2-chloro substituent in the compound of formula (15) can be reacted with Pd(PPh3)4 (CAS: 14221-01-3), PdCl2(dppe) (CAS: 19978-61-1), Pd in ​​a suitable solvent such as 1,4-dioxane, HO, THF or DME, or a mixture of suitable solvents, at a temperature between about room temperature and about 200°C by heating using conventional heating, or optionally with microwave irradiation, in an open or optionally sealed vessel, optionally at a pressure greater than atmospheric pressure. It can be substituted with a methyl group using a transition metal catalyzed coupling reaction such as treatment with MeB(OH)2, MeBPin (CAS:94242-85-0) or trimethylboroxine (CAS:823-96-1) in the presence of a palladium catalyst such as (dppf)Cl2 (CAS:72287-26-4) or Pd2(dba)3 (CAS:51364-51-3), optionally in the presence of a phosphine ligand such as P(Cy)3, in the presence of an inorganic base such as K2CO3 or Cs2CO3. Alternatively, other methods known to those skilled in the art can be used, such as MeMgBr or MeMgCl in combination with a nickel catalyst, such as Ni(dppf)Cl (CAS: 67292-34-6), in a solvent such as THF, EtO, DME, or 1,4-dioxane at elevated temperatures; MeZn in combination with a palladium catalyst, such as PdCl(dppe) (CAS: 19978-61-1) or Pd(dppf)Cl (CAS: 72287-26-4), in a solvent such as toluene or 1,4-dioxane at elevated temperatures; MeAl in combination with a palladium catalyst, such as Pd(PPh) (CAS: 14221-01-3), in a solvent such as THF, hexane, or heptane at elevated temperatures; or MeSn in combination with a palladium catalyst, such as Pd(PPh) (CAS: 14221-01-3), in a solvent such as THF or DMF at elevated temperatures.

[0107] [ka]

[0108] (E) Converting one compound of formula (1) into another compound of formula (1): Furthermore, one compound of formula (1) can be converted into another compound of formula (1) by methods well known to those skilled in the art. Examples of synthetic procedures for converting one functional group into another are described in standard textbooks such as March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, 7th Edition, Michael B. Smith, John Wiley, 2013, (ISBN: 978-0-470-46259-1), Organic Syntheses, Online Edition, www.orgsyn.org, (ISSN 2333-3553), and Fiesers' Reagents for Organic Synthesis, Volumes 1-17, John Wiley, edited by Mary Fieser (ISBN: 0-471-58283-2).

[0109] In many of the above reactions, it may be necessary to protect one or more groups to prevent the reaction from occurring at an undesired location on the molecule. Examples of protecting groups and methods for protecting and deprotecting functional groups can be found in Greene's Protective Groups in Organic Synthesis, Fifth Edition, Editor: Peter GM Wuts, John Wiley, 2014, (ISBN: 9781118057483).

[0110] The compounds made by the above-described methods can be isolated and purified by any of a variety of methods well known to those skilled in the art, examples of which include recrystallization and chromatographic techniques such as column chromatography (e.g., flash chromatography), HPLC and SFC under normal or reverse phase conditions.

[0111] Pharmaceutical preparations While it is possible for the active compound to be administered alone, it is preferable to supply it as a pharmaceutical composition (eg, formulation).

[0112] Therefore, in another embodiment of the present invention, there is provided a pharmaceutical composition comprising at least one compound of formula (1) as defined above, together with at least one pharmaceutically acceptable excipient.

[0113] The composition may be a tablet composition.

[0114] The composition may be a capsule composition.

[0115] Pharmaceutically acceptable excipients can be selected from, for example, carriers (e.g., solid, liquid, or semi-solid carriers), adjuvants, diluents (e.g., solid diluents such as fillers or bulking agents; and liquid diluents such as solvents and co-solvents), granulating agents, binders, flow aids, coating agents, release-controlling agents (e.g., release-suppressing or retarding polymers, or waxes), binding agents, disintegrants, buffering agents, lubricants, preservatives, antifungal and antibacterial agents, antioxidants, buffering agents, tonicity adjusting agents, thickeners, flavoring agents, sweetening agents, pigments, plasticizers, taste-masking agents, stabilizers, or any other excipients conventionally used in pharmaceutical compositions.

[0116] The term "pharmaceutically acceptable," as used herein, means compounds, substances, compositions, and / or dosage forms that are suitable for use in contact with the tissues of a subject (e.g., a human subject) without undue toxicity, irritation, allergic response, or other problem or complication, and that are, within the scope of sound medical judgment, commensurate with a reasonable benefit / risk ratio. Each excipient must also be "acceptable" in the sense of being compatible with the other ingredients of the formulation.

[0117] Pharmaceutical compositions containing compounds of formula (1) can be formulated according to known techniques, see, for example, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, PA, USA.

[0118] The pharmaceutical compositions can be in any form suitable for oral, parenteral, topical, intranasal, intrabronchial, sublingual, ocular, otic, rectal, vaginal or transdermal administration.

[0119] Pharmaceutical dosage forms suitable for oral administration include tablets (coated or uncoated), capsules (hard or soft shell), caplets, pills, lozenges, syrups, liquids, powders, granules, elixirs and suspensions, sublingual tablets, wafers, or patches such as buccal patches.

[0120] The tablet composition can contain a unit dose of the active compound together with an inert diluent or carrier, such as a sugar or sugar alcohol, for example, lactose, sucrose, sorbitol, or mannitol; and / or a non-sugar-derived diluent, such as sodium carbonate, calcium phosphate, calcium carbonate, or a cellulose or derivative thereof, such as microcrystalline cellulose (MCC), methylcellulose, ethylcellulose, hydroxypropylmethylcellulose, and a starch, such as corn starch. Tablets may also contain standard ingredients such as binders and granulating agents, such as polyvinylpyrrolidone, disintegrants (e.g., swellable cross-linked polymers, such as cross-linked carboxymethylcellulose), lubricants (e.g., stearates), preservatives (e.g., parabens), antioxidants (e.g., BHT), buffers (e.g., phosphate buffer or citrate buffer), and effervescent agents, such as citrate / bicarbonate mixtures. Such excipients are well known and need not be discussed in detail here.

[0121] Tablets may be designed to release drug upon contact with gastric fluids (immediate-release tablets) or in a controlled manner over an extended period of time or upon contact with specific areas of the GI tract (controlled-release tablets).

[0122] The pharmaceutical compositions typically contain about 1% (w / w) to about 95%, preferably % (w / w), of the active ingredient and 99% (w / w) to 5% (w / w) of a pharmaceutically acceptable excipient (e.g., as defined above), or a combination of such excipients. Preferably, the compositions contain about 20% (w / w) to about 90% of the active ingredient and 80% (w / w) to 10% of a pharmaceutical excipient, or a combination of excipients. The pharmaceutical compositions contain about 1% to about 95%, preferably about 20% to 90%, of the active ingredient. The pharmaceutical compositions according to the present invention may be in unit dose form, such as in the form of ampoules, vials, suppositories, pre-filled syringes, dragees, powders, tablets, or capsules.

[0123] Tablets and capsules may contain, for example, 0-20% disintegrant, 0-5% lubricant, 0-5% flow aid, and / or 0-99% (w / w) filler / bulking agent (depending on drug dose). Tablets and capsules may contain 0-10% (w / w) polymer binder, 0-5% (w / w) antioxidant, and 0-5% (w / w) pigment. Slow-release tablets typically also contain 0-99% (w / w) release-controlling (e.g., retarding) polymer (depending on dose). Film coatings for tablets or capsules typically contain 0-10% (w / w) polymer, 0-3% (w / w) pigment, and / or 0-2% (w / w) plasticizer.

[0124] Parenteral formulations typically contain 0-20% (w / w) buffer, 0-50% (w / w) cosolvent, and / or 0-99% (w / w) water for injection (WFI) (depending on dose, if lyophilized). Formulations for intramuscular depots may also contain 0-99% (w / w) oil.

[0125] The pharmaceutical formulations may be supplied to the patient in a "patient pack" containing an entire course of treatment in a single package, usually a blister pack.

[0126] The compounds of formula (1) are generally supplied in unit dosage form and, thus, typically contain sufficient compound to achieve a desired level of biological activity. For example, a formulation may contain 1 nanogram to 2 grams of active ingredient, e.g., 1 nanogram to 2 milligrams of active ingredient. Within these ranges, particular subranges of compound are 0.1 milligram to 2 grams of active ingredient (more generally, 10 milligrams to 1 gram, e.g., 50 milligrams to 500 milligrams), or 1 microgram to 20 milligrams (e.g., 1 microgram to 10 milligrams, e.g., 0.1 milligram to 2 milligrams of active ingredient).

[0127] For oral compositions, a unit dosage form may contain from 1 milligram to 2 grams, more usually 10 milligrams to 1 gram, for example, 50 milligrams to 1 gram, for example, 100 milligrams to 1 gram of active compound.

[0128] The active compound is administered to a patient (e.g., a human or animal patient) in need thereof in an amount sufficient to achieve the desired therapeutic effect (effective amount). The exact amount of compound to be administered can be determined by the supervising physician according to standard procedures. [Example]

[0129] The present invention will now be illustrated, but not limited to, by reference to the following examples.

[0130] [Examples 1-1 to 17-15] The compounds of Examples 1-1 to 17-15 shown below in Table 1 were prepared. The NMR and LCMS characteristics and methods used to prepare them are described in Table 3. The starting materials are listed in Table 2.

[0131] [Table 1-1]

[0132] [Table 1-2]

[0133] [Table 1-3]

[0134] [Table 1-4]

[0135] [Table 1-5]

[0136] General Procedure Preparative routes are not included, but relevant intermediates are commercially available. Commercially available reagents were utilized without further purification. Final compounds and intermediates are named using ChemDraw Professional, version 17.0.0.206(121). Room temperature (RT) refers to approximately 20-27 °C. 1 H NMR spectra were recorded at 400 or 500 MHz on either Bruker, Varian, or Jeol instruments. Chemical shift values ​​are expressed in parts per million (ppm), or σ values, relative to the following solvents: chloroform - d = 7.26 ppm, DMSO - d = 2.50 ppm, and methanol - d = 3.31 ppm. The following abbreviations are used for NMR signal multiplicity: s = singlet, br = broad, d = doublet, t = triplet, q = quartet, and m = multiplet. Coupling constants are listed as J values ​​measured in Hz. NMR and mass spectroscopy results were corrected for background peaks. Chromatography refers to column chromatography performed using 60-120 mesh or 40-633 μm, 60 Å silica gel under nitrogen pressure (flash chromatography). PL-HCO3MP SPE refers to StratoSpheres HCO3, available from Polymer Laboratories. -Refers to bonded macroporous polystyrene solid-phase extraction cartridges. Microwave-mediated reactions were performed in a Biotage Initiator or CEM Discover microwave reactor.

[0137] LCMS analysis LCMS analysis of compounds was performed under electrospray conditions using the equipment and methods shown in the table below:

[0138] [Table 2]

[0139] [Table 3-1]

[0140] [Table 3-2]

[0141] The experimental entries and LCMS data in Tables 2 and 3 are presented in the following format: (instrument system, method): mass ion, retention time, UV detection wavelength.

[0142] compound purification Final purification of compounds was achieved by reversed-phase column chromatography, preparative reversed-phase HPLC, chiral HPLC or chiral SFC using the equipment and methods detailed below, and data are presented in the following format: Purification technique: [Phase (column description, column length x internal diameter, particle size), solvent flow rate, gradient - expressed as % of mobile phase B in mobile phase A (over time), mobile phase (A), mobile phase (B)].

[0143] Reverse-phase column chromatography Teledyne Isco instrument using a pre-packed disposable silica-based C18 (17%) / Silicycle / 40–63 μm, 60 Å stationary phase column with an eluent flow rate range of 15–200 mL / min and UV detection (254 and 280 nm).

[0144] Preparative HPLC purification: Shimadzu LC-20AP binary system with SPD-20A UV detector Mass spectrometers operated on a Waters 2767 equipped with a PDA detector and a Waters ZQ equipped with an electrospray ion source operated in positive ion mode.

[0145] Chiral HPLC purification: Shimadzu LC-20AP binary system with SPD-20A UV detector

[0146] Chiral SFC purification: Waters SFC200 Purification method A Preparative HPLC: [reverse phase (BEH C-18, 50 × 30 mm, 5 μm), 40 mL / min, gradient 5% (0.5 min), 5% to 25% (6.4 min), 100% (1.6 min), 100% to 5% (0.5 min), mobile phase (A): 10 mM ammonium carbonate in water, pH 10, (B): 100% acetonitrile].

[0147] Purification method B Preparative HPLC: [reverse phase (BEH C-18, 50 × 30 mm, 5 μm), 40 mL / min, gradient 10% (0.5 min), 10% to 30% (6.4 min), 100% (1.6 min), 100% to 10% (0.5 min), mobile phase (A): 10 mM ammonium carbonate in water, pH 10, (B): 100% acetonitrile].

[0148] Purification method C Preparative HPLC: [reverse phase (BEH C-18, 50 × 30 mm, 5 μm), 40 mL / min, gradient 10% (0.5 min), 10% to 30% (6.4 min), 100% (1.6 min), 100% to 10% (0.5 min), mobile phase (A): 10 mM ammonium bicarbonate in water, pH 10, (B): 100% acetonitrile].

[0149] Purification method D Preparative HPLC: [reverse phase (BEH C-18, 50 × 30 mm, 5 μm), 40 mL / min, gradient 22% (0.5 min), 22% to 42% (6.4 min), 100% (1.6 min), 100% to 22% (0.5 min), mobile phase (A): 10 mM ammonium carbonate in water, pH 10, (B): 100% acetonitrile].

[0150] Purification method E Preparative HPLC: [Reverse phase (Gemini-NX 30x150 mm, 5 μm), 40 mL / min, gradient 10% (0.5 min), 10% to 100% (6.4 min), 100% (1.6 min), 100% to 10% (0.5 min), mobile phase (A): 10 mM ammonium bicarbonate in water, pH 10, (B): 100% acetonitrile].

[0151] Purification method F Preparative HPLC: [Reverse phase (X-BRIDGE C-18, 250 × 19 mm, 5 μm), 11 mL / min, gradient 10% to 32% (30 min), 32% (4 min), 100% (2 min), 100% to 10% (6 min), mobile phase (A): 5 mM ammonium bicarbonate + 0.1% ammonia in water, (B): 100% acetonitrile].

[0152] Purification method G Preparative HPLC: [reverse phase (BEH C-18, 150 × 30 mm, 5 μm), 40 mL / min, gradient 80% (0.5 min), 80% to 100% (6.4 min), 100% (1.6 min), 100% to 80% (0.5 min), mobile phase (A): 10 mM ammonium bicarbonate in water, pH 10, (B): 100% acetonitrile].

[0153] Abbreviations used throughout this specification Ac = acetate aq. = aqueous Bn = benzyl Bz = benzoyl Boc = tert-butyloxycarbonyl n BuOH = n-butanol t BuOH = t-butanol Bu3P = tri-n-butylphosphine t Bu3P = tri-tert-butylphosphine Cbz = benzyloxycarbonyl COMU = (1-cyano-2-ethoxy-2-oxoethylideneaminooxy)dimethylamino-morpholino-carbenium hexafluorophosphate DCM = dichloromethane DEAD = diethyl azodicarboxylate DIAD = diisopropyl azodicarboxylate DIC = N,N'-diisopropylcarbodiimide DIPEA = N,N-diisopropylethylamine DMA = N,N-dimethylacetamide DMAP = 4-(dimethylamino)pyridine DMB = 3,4-dimethoxybenzyl DME = dimethoxyethane DMF = N,N-dimethylformamide DMSO = dimethyl sulfoxide EDC = 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide ES = electrospray ionization Et3N = triethylamine Et2O = diethyl ether EtOAc = ethyl acetate EtOH = ethanol Fmoc = fluorenylmethyloxycarbonyl h=time HATU = 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate H2O = Water HCl = hydrogen chloride, hydrochloric acid HOBt = hydroxybenzotriazole HPLC = High-Performance Liquid Chromatography IPA = Propan-2-ol LC = liquid chromatography MeCN = acetonitrile MeOH = methanol min=minutes MS=mass spectrometry nm = nanometers NMP = N-methyl-2-pyrrolidone NMR=nuclear magnetic resonance P(Cy)3 = tricyclohexylphosphine PMB = 4-methoxybenzyl PPh3, Ph3P = triphenylphosphine PTSA = para-toluenesulfonic acid PyBOP = (benzotriazol-1-yloxy)tripyrrolidinophosphonium Hexafluorophosphate RP-flash = reversed-phase flash chromatography RP-HPLC = reversed-phase high-performance liquid chromatography RT=room temperature sat.=saturated SFC = Supercritical Fluid Chromatography SNAr = nucleophilic aromatic substitution TBAF = tetrabutylammonium fluoride TEA = triethylamine Teoc = β-(trimethylsilyl)ethoxycarbonyl TFA = trifluoroacetic acid TFAA = trifluoroacetic anhydride THF = tetrahydrofuran TMAD = N,N,N',N'-tetramethylazodicarboxamide T3P = 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxide

[0154] Synthesis of intermediates: Route 1 Typical Procedure for the Preparation of Intermediate 2, 2,4,6-Trichloropyrimidin-5-ol

[0155] [ka] Under a nitrogen atmosphere, a solution of 2,4,6-trichloro-5-methoxypyrimidine (Intermediate 1) (2.60 g, 12.2 mmol) in DCM (121 mL) was cooled to 0 °C and treated dropwise with boron tribromide (neat, 4.05 mL, 42.6 mmol). The reaction mixture was stirred at room temperature for 18 h, then cooled below 0 °C and carefully quenched with methanol (20 mL), then diluted with water (120 mL) and the phases separated. The aqueous layer was extracted with DCM (3 × 100 mL), and the combined organic extracts were dried (NaSO), filtered, and concentrated in vacuo to give a light tan solid (2.27 g). The crude material was purified by flash chromatography on silica gel (80 g cartridge) using 0% to 20% EtOAc in DCM to afford 2,4,6-trichloropyrimidin-5-ol (Intermediate 2) (1.67 g, 62%) as a white solid. Data for intermediate 2 are in Table 2.

[0156] Route 2 Typical Procedure for the Preparation of Protected Amino Alcohols, Illustrated by the Preparation of Intermediate 10, tert-Butyl (R)-(1-cyclopropyl-2-hydroxyethyl)carbamate

[0157] [ka]

[0158] (R)-2-((tert-Butoxycarbonyl)amino)-2-cyclopropylacetic acid (Intermediate 9) (2 g, 0.009 mol) was dissolved in THF (20.0 mL) and cooled to 0 °C. A solution of borane THF complex in THF (1.0 M, 32 mL, 0.032 mol) was added dropwise at 0 °C, and the resulting mixture was stirred at room temperature for 16 h. The reaction mixture was quenched by the addition of methanol, then the solvent was removed in vacuo, and the residue was partitioned between HO (50 mL) and EtOAc (30 mL), and the phases were separated. The aqueous layer was further extracted with EtOAc (2 × 50 mL), and the combined organic layers were washed with saturated aqueous NaHCO solution. The organic layer was dried (NaSO) and the solvent removed in vacuo to give the crude product, which was purified by column chromatography (normal phase 60-120 mesh silica gel, 0-30% EtOAc in hexanes) to give tert-butyl (R)-(1-cyclopropyl-2-hydroxyethyl)carbamate (Intermediate 10) (1.8 g, 96%) as a colorless gum. Data for intermediate 10 are in Table 2.

[0159] Route 3 Typical procedure for the preparation of protected amino alcohols, exemplified by the preparation of intermediate 14, tert-butyl ((2S,3R)-1-hydroxy-3-methoxybutan-2-yl)carbamate

[0160] [ka]

[0161] To a stirred solution of D-allothreonine (3.0 g, 25.2 mmol) in THF (41 mL) and HO (41 mL) was added a solution of sodium carbonate (5.60 g, 52.8 mmol) in HO (13 mL). The resulting mixture was stirred for 5 minutes, followed by the addition of di-tert-butyl dicarbonate (6.59 g, 30.2 mmol). The mixture was stirred overnight at room temperature, then water (15 mL) was added, and the mixture was extracted with diethyl ether (2 × 10 mL). The aqueous layer was acidified to pH = 4 with 1 M aqueous HCl and extracted with EtOAc (3 × 25 mL). The combined EtOAc layers were dried (NaSO) and concentrated in vacuo to give (tert-butoxycarbonyl)-D-allothreonine (5.10 g, 92%) as a white solid. H NMR (500 MHz, chloroform-d) δ 1.23–1.36 (m, 3H), 1.45 (s, 9H), 4.06–4.24 (m, 1H), 4.30–4.41 (m, 1H), 5.58 (d, J = 7.4 Hz, 1H), 6.16 (br. s, 2H).

[0162] To a stirred solution of (tert-butoxycarbonyl)-D-allothreonine (5.10 g, 23.3 mmol) in dry acetonitrile (320 mL) at room temperature, AgO (26.95 g, 116.0 mmol) and methyl iodide (14.5 mL, 233.0 mmol) were added, and the resulting mixture was stirred in the dark for 4 days. The mixture was filtered through Celite and washed several times with DCM. The filtrate was concentrated in vacuo to give the crude product, which was purified by flash chromatography (0–100% EtOAc in hexanes, 80 g SiO cartridge) to give methyl N-(tert-butoxycarbonyl)-O-methyl-D-allothreoninate as a colorless liquid (3.80 g, 66%). 1H NMR (400 MHz, chloroform-d) δ 1.20 (d, J = 6.5 Hz, 3H), 1.45 (s, 9H), 3.36 (s, 3H), 3.59–3.67 (m, 1H), 3.76 (s, 3H), 4.39–4.46 (m, 1H), 5.24–5.30 (m, 1H).

[0163] To a stirred solution of methyl N-(tert-butoxycarbonyl)-O-methyl-D-allothreoninate (3.80 g, 15.4 mmol) in THF (30 mL) under nitrogen at 0 °C was added a solution of LiBH in THF (2.0 M, 11.5 mL, 23.1 mmol), and the mixture was allowed to warm slowly to room temperature and stirred overnight. The reaction mixture was quenched with saturated aqueous NH Cl (15 mL) and extracted with ethyl acetate (3 × 25 mL). The combined organic extracts were dried over sodium sulfate and concentrated in vacuo. The residue was purified by flash chromatography (0–100% EtOAc in hexanes, 80 g SiO cartridge) to afford tert-butyl ((2S,3R)-1-hydroxy-3-methoxybutan-2-yl)carbamate (Intermediate 14) (3.10 g, 92%) as a colorless liquid. Data for intermediate 14 are in Table 2.

[0164] Route 4 Typical procedure for the preparation of protected amino alcohols, exemplified by the preparation of intermediate 18, tert-butyl (S)-(1-hydroxy-3-methoxy-3-methylbutan-2-yl)carbamate

[0165] [ka]

[0166] A solution of methyl iodide (3.6 mL, 57.8 mmol) in dry EtO (39 mL) was slowly added to magnesium turnings (1.17 g, 48.2 mmol) in dry EtO (10 mL). After complete consumption of the magnesium, a solution of 3-(tert-butyl) 4-methyl(S)-2,2-dimethyloxazolidine-3,4-dicarboxylate (Intermediate 17) (5 g, 19.3 mmol) in dry EtO (20 mL) was added dropwise at such a rate that the solution began to reflux. After complete addition, the reaction mixture was stirred for an additional 10 min, and then saturated aqueous NH4Cl (70 mL) was carefully added. The layers were separated, and the aqueous layer was extracted with EtO (2 × 70 mL). The combined organic extracts were dried (MgSO), filtered, and concentrated under reduced pressure to give tert-butyl (S)-4-(2-hydroxypropan-2-yl)-2,2-dimethyloxazolidine-3-carboxylate (4.62 g, 92%) as a colorless oil, which was used directly in the next step without any purification. 1 H NMR (400 MHz, chloroform-d) δ 1.16 (s, 3H), 1.17 (s, 3H), 1.49 (s, 9H), 1.50 (s, 3H), 1.58 (s, 3H), 3.72–3.82 (m, 1H), 3.92–4.02 (m, 2H), 5.25 (s, 1H).

[0167] To a solution of tert-butyl (S)-4-(2-hydroxypropan-2-yl)-2,2-dimethyloxazolidine-3-carboxylate (5.12 g, 19.7 mmol) in DMF (19.0 mL) cooled to 0 °C, NaH (60% dispersion in mineral oil, 1.02 g, 25.6 mmol) and methyl iodide (2.46 mL, 39.5 mmol) were added, and the reaction mixture was stirred at room temperature for 2 h. MeOH (2.0 mL) was added to quench the reaction, and the mixture was then diluted with DCM (50.0 mL) and washed twice with water. The organic layer was dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–100%) in hexanes to afford tert-butyl (S)-4-(2-methoxypropan-2-yl)-2,2-dimethyloxazolidine-3-carboxylate (4.9 g, 91%) as a colorless oil that crystallized on standing. 1 H NMR (400 MHz, chloroform-d) δ 1.13 (s, 3H), 1.19 (s, 3H), 1.48 (s, 9H), 1.49 (s, 3H), 1.61 (s, 3H), 3.21 (s, 3H), 3.83–3.89 (m, 1H), 3.90–4.11 (m, 1H), 4.12–4.18 (m, 1H).

[0168] To a stirred solution of tert-butyl (S)-4-(2-methoxypropan-2-yl)-2,2-dimethyloxazolidine-3-carboxylate (3.60 g, 13.2 mmol) in MeOH (55.0 mL) was added PTSA (250 mg, 1.32 mmol), and the resulting mixture was stirred at room temperature for 30 minutes. The reaction was quenched with saturated aqueous NaHCO (10 mL). The solvent was removed under reduced pressure, and HO (15 mL) was added. The aqueous layer was extracted with EtOAc (3 × 25 mL), and the combined organic extracts were dried (NaSO), filtered, and concentrated under reduced pressure to give tert-butyl (S)-(1-hydroxy-3-methoxy-3-methylbutan-2-yl)carbamate (Intermediate 18) (3.0 g, 98%) as a white solid. Data for intermediate 18 are in Table 2.

[0169] Route 5 Typical Procedure for the Preparation of Protected Amino Alcohols, Illustrated by the Preparation of Intermediate 20, 2-(Trimethylsilyl)ethyl (2-hydroxy-1-(oxetan-3-yl)ethyl)carbamate

[0170] [ka]

[0171] Under N2, a 500 mL flask was charged with methyl 2-(((benzyloxy)carbonyl)amino)-2-(oxetan-3-ylidene)acetate (Intermediate 19) (4.06 g, 14.6 mmol) and MeOH (240 mL). Mg chips (3.56 g, 146 mmol) were then added, and the mixture was stirred at room temperature for 3 h (Note: copious H2 evolution was observed). The resulting mixture was cooled to 0 °C, and aqueous saturated NH4Cl (85 mL) was added slowly and carefully. The mixture was concentrated under reduced pressure until most of the MeOH was removed, and the residue was extracted with DCM (3 x 60 mL). The combined organic phase was washed with brine (50 mL), dried over MgSO4, and concentrated under reduced pressure. The residue was purified by column chromatography (dry load, 80 g SiO, 0:100 to 90:10, EtOAc:hexanes) to afford methyl 2-(((benzyloxy)carbonyl)amino)-2-(oxetan-3-yl)acetate (2.41 g, 59%) as a colorless solid. 1 H NMR (400 MHz, DMSO-d6) δ 3.20 - 3.37 (m, 1H), 3.62 (s, 3H), 4.35 (t, J = 6.3 Hz, 1H), 4.38 - 4.48 (m, 2H), 4.50 - 4.62 (m, 2H), 5.05 (s, 2H), 7.27 - 7.41 (m, 5H), 7.86 (d, J = 8.0 Hz, 1H).

[0172] Under N2, a 100 mL flask was charged with methyl 2-(((benzyloxy)carbonyl)amino)-2-(oxetan-3-yl)acetate (2.35 g, 8.41 mmol) and anhydrous THF (35 mL). The resulting solution was cooled to 0 °C, and then a solution of LiBH4 in THF (2 M, 8.4 mL, 16.8 mmol) was added dropwise. The resulting mixture was stirred at 0 °C for 30 min, and then the mixture was further stirred at room temperature for 2 h. The resulting mixture was cooled to 0 °C and carefully quenched by the addition of water (20 mL), further stirred at room temperature for 30 min, EtOAc (40 mL) was added, the phases were separated, and the aqueous phase was extracted with EtOAc (2 × 20 mL). The combined organic phases were dried over MgSO4 and concentrated under reduced pressure to give benzyl (2-hydroxy-1-(oxetan-3-yl)ethyl)carbamate (1.90 g, 90%) as a colorless solid. 1 H NMR (500 MHz, DMSO-d6) δ 3.04 - 3.13 (m, 1H), 3.20 - 3.27 (m, 1H), 3.30 - 3.36 (m, 1H), 3.78 - 3.85 (m, 1H), 4.33 - 4.41 (m, 2H), 4.48 - 4.57 (m, 2H), 4.65 (t, J = 5.6 Hz, 1H), 5.03 (s, 2H), 7.15 (d, J = 8.8 Hz, 1H), 7.29 - 7.33 (m, 1H), 7.33 - 7.39 (m, 4H).

[0173] A 100 mL flask was charged with 10% Pd / C (178 mg, 0.167 mmol Pd), and the flask was then purged with N. A solution of benzyl (2-hydroxy-1-(oxetan-3-yl)ethyl)carbamate (1.68 g, 6.69 mmol) in MeOH (34 mL) was then added. The atmosphere was replaced with H by four evacuation / H cycles, and the suspension was stirred under H (1 atm) at room temperature for 5 h. The atmosphere in the flask was replaced with N, and the suspension was filtered over Celite, and the solid residue was washed several times with MeOH. The filtrate was concentrated under reduced pressure to give 2-amino-2-(oxetan-3-yl)ethan-1-ol (784 mg, 100%) as a colorless oil. 1 H NMR (400 MHz, chloroform-d) δ 2.90 - 3.00 (m, 1H), 3.18 - 3.29 (m, 2H), 3.50 - 3.56 (m, 1H), 4.47 (t, J = 6.2 Hz, 1H), 4.58 (t, J = 6.2 Hz, 1H), 4.73 - 4.83 (m, 2H). Three exchangeable protons are not observed.

[0174] A 200 mL flask was charged with 2-amino-2-(oxetan-3-yl)ethan-1-ol (784 mg, 6.69 mmol) and 1,4-dioxane (57 mL) under N2. Next, Et3N (1.4 mL, 10.0 mmol) was added, followed by a solution of 2,5-dioxopyrrolidin-1-yl(2-(trimethylsilyl)ethyl)carbonate (CAS: 78269-85-9) (1.77 g, 6.82 mmol) in 1,4-dioxane (10 mL). A thick suspension immediately formed and was stirred at room temperature for 16 h. The resulting clear solution was concentrated under reduced pressure, and the residue was dissolved in a mixture of EtOAc (75 mL) and saturated aqueous NH4Cl (50 mL). The phases were separated, and the aqueous phase was extracted with EtOAc (2 × 25 mL). The combined organic phases were washed with brine (25 mL), dried over MgSO, and concentrated under reduced pressure. The residue was purified by column chromatography (dry load, 40 g SiO, 50:50 to 100:0, EtOAc:hexanes) to afford 2-(trimethylsilyl)ethyl (2-hydroxy-1-(oxetan-3-yl)ethyl)carbamate (Intermediate 20) (1.68 g, 96%) as a colorless oil. Data for intermediate 20 are in Table 2.

[0175] Route 6 Typical procedure for the preparation of protected amino alcohols, exemplified by the preparation of intermediate 26, tert-butyl (2,6-dichloro-5-hydroxypyrimidin-4-yl)((2R,3S)-3-hydroxybutan-2-yl)carbamate

[0176] [ka]

[0177] To a solution of 2,4-dichloro-6-(((2R,3S)-3-hydroxybutan-2-yl)amino)pyrimidin-5-ol (Intermediate 25) (1.02 g, 4.05 mmol) in a mixture of THF (40 mL) and water (20 mL) was added di-tert-butyl dicarbonate (927 mg, 4.25 mmol) and NaHCO3 (714 mg, 8.5 mmol) and the reaction mixture was stirred at room temperature for 18 hours. The mixture was then concentrated under reduced pressure, and the residue was directly purified by silica gel chromatography (dry-loaded) using a gradient of 0-100% EtOAc in hexanes followed by a gradient of 0-15% MeOH in DCM to afford tert-butyl (2,6-dichloro-5-hydroxypyrimidin-4-yl)((2R,3S)-3-hydroxybutan-2-yl)carbamate (Intermediate 26) (155 mg, 11%) as a colorless oil and tert-butyl (2,4-dichloro-6-(((2R,3S)-3-hydroxybutan-2-yl)amino)pyrimidin-5-yl)carbonate (350 mg, 25%) as a pale yellow oil. The undesired O-Boc product could be converted separately to the desired N-Boc product by stirring with NaHCO in THF / HO. Data for intermediate 26 are in Table 2.

[0178] Route 7 Typical procedure for the preparation of protected amino alcohols exemplified by the preparation of intermediate 36, tert-butyl (1,1,1-trifluoro-3-hydroxypropan-2-yl)carbamate

[0179] [ka]

[0180] Under N2, a 100 mL flask was charged with methyl 2-((tert-butoxycarbonyl)amino)-3,3,3-trifluoro-2-hydroxypropanoate (Intermediate 35) (2.71 g, 9.92 mmol) and anhydrous Et2O (50 mL). The resulting mixture was cooled to 0 °C, and then TFAA (1.40 mL, 9.92 mmol) and pyridine (1.60 mL, 19.8 mmol) were added. The mixture was stirred at 0 °C for 1 h, and then at room temperature for an additional 16 h. The resulting suspension was filtered, and the solid was washed with Et2O (2 × 25 mL). The filtrate was washed with water (25 mL), dried over MgSO4, and concentrated under reduced pressure to give crude methyl 2-((tert-butoxycarbonyl)imino)-3,3,3-trifluoropropanoate (2.51 g, 99%) as a clear oil, which was used in the next step without further purification. 1 H NMR (500 MHz, chloroform-d) δ 1.58 (s, 9H), 3.95 (s, 3H).

[0181] A 100 mL oven-dried flask under N was charged with methyl 2-((tert-butoxycarbonyl)imino)-3,3,3-trifluoropropanoate (2.51 g, 9.84 mmol) and anhydrous EtO (30 mL). The resulting solution was cooled to -78 °C, and then a solution of LiAlH in THF (2 M, 9.84 mL, 19.7 mmol) was slowly added. The mixture was then stirred for 16 h while slowly warming to room temperature. The resulting solution was cooled to 0 °C and carefully quenched by the sequential addition of water (0.75 mL), aqueous NaOH (3.8 M, 0.75 mL), and water (2.3 mL). The resulting suspension was warmed to room temperature, and MgSO was added. The suspension was filtered, and the solid was washed with THF (4 × 20 mL). The filtrate was concentrated under reduced pressure, and the residue was purified by column chromatography (dry pour, 40 g SiO, 5:95 to 70:30, EtOAc:hexanes) to afford tert-butyl (1,1,1-trifluoro-3-hydroxypropan-2-yl)carbamate (Intermediate 36) (1.38 g, 61%) as a colorless solid. Data for intermediate 36 are in Table 2.

[0182] Route 8 Typical Procedure for the Preparation of Protected Amino Acids, Illustrated by the Preparation of Intermediate 61, 2-(((tert-butoxycarbonyl)amino)methyl)butanoic Acid

[0183] [ka]

[0184] 2-(Aminomethyl)butanoic acid (Intermediate 60) (1.5 g, 9.8 mmol) was dissolved in DCM (5.0 mL) and TEA (379 mg, 14.7 mmol) was added dropwise at 0 °C. Di-tert-butyl dicarbonate (512 mg, 11.7 mmol) was then added and the reaction mixture was stirred at room temperature for 1 h. The solvent was removed in vacuo and the residue was partitioned between HO (100 mL) and DCM (60 mL). The aqueous layer was washed with 1 M citric acid solution and then further extracted with DCM (2 × 60 mL). All organic layers were combined and dried (NaSO) and the solvent removed in vacuo to give the crude product, which was purified by column chromatography on silica using 16% EtOAc in hexanes to give 2-(((tert-butoxycarbonyl)amino)methyl)butanoic acid (Intermediate 61) (2.4 g, 86%) as a colorless gum. Data for intermediate 61 are in Table 2.

[0185] General synthetic procedure: Route A Typical Procedure for the Preparation of Fused Pyrimidines, Illustrated by Example 1-1, Preparation of (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine

[0186] [ka]

[0187] To a solution of 2,4,6-trichloropyrimidin-5-ol (Intermediate 2) (384 mg, 1.926 mmol) and tert-butyl (2-hydroxyethyl)carbamate (Intermediate 3) (621 mg, 3.851 mmol) in THF (11.0 mL) cooled to 0 °C, DIAD (0.57 mL, 2.888 mmol) and PPh3 (758 mg, 2.888 mmol) were added, and the reaction mixture was stirred for 2 h. The THF was then removed under reduced pressure, and silica was added. The residue was purified by silica gel chromatography (dry-packed) using a gradient of 0–60% EtOAc in hexanes to afford tert-butyl (2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)carbamate (633 mg, 96%) as a white solid. 1 H NMR (500 MHz, chloroform-d) δ 1.45 (s, 9H), 3.56 (q, J = 5.5 Hz, 2H), 4.17 (t, J = 5.0 Hz, 2H), 5.05 (s, 1H).

[0188] To a solution of tert-butyl (2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)carbamate (633 mg, 1.848 mmol) in DCM (5.10 mL) was added TFA (5.10 mL), and the reaction mixture was stirred at room temperature for 10 minutes. The mixture was then concentrated in vacuo to dryness to afford 2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethan-1-amine trifluoroacetate (675 mg, >100%) as a colorless oil, which was used directly in the next step without any purification. LCMS (System 1, Method D): m / z 242 / 244 (M+H) + (ES + ), 0.54 min, 190~320nm.

[0189] To a solution of 2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethan-1-amine trifluoroacetate (659 mg, 1.848 mmol) in 1,4-dioxane (5.00 mL) was added DIPEA (0.97 mL, 5.545 mmol), and the reaction mixture was heated at 80 °C for 18 h. The mixture was then diluted with water and extracted with EtOAc (3 × 10.0 mL). The combined organic extracts were dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry-packed) using a gradient of 0–100% EtOAc in hexanes to afford 2,4-dichloro-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine (279 mg, 73%) as a white solid. LCMS (System 1, Method D): m / z 206 / 208 (M+H) + (ES + ), 1.87 min, 190~320nm.

[0190] To a solution of 2,4-dichloro-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine (279 mg, 1.354 mmol) in 1,4-dioxane (2.90 mL) was added tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (271 mg, 1.354 mmol) and DIPEA (0.47 mL, 2.708 mmol), and the mixture was heated to 80° C. for 18 hours. After cooling to room temperature, the mixture was diluted with water and extracted with EtOAc (3×10.0 mL). The combined organic extracts were dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography using a gradient of EtOAc (0–100%) in hexanes to afford tert-butyl (R)-(1-(2-chloro-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (218 mg, 44%) as a white solid. LCMS (System 1, Method D): m / z 370 / 372 (M+H) + (ES + ), 2.40 min, 190~320nm.

[0191] To a solution of tert-butyl (R)-(1-(2-chloro-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (127 mg, 0.343 mmol) in a mixture of THF (0.83 mL) and DMF (0.22 mL), di-tert-butyl dicarbonate (112 mg, 0.515 mmol), EtN (0.102 mL, 0.755 mmol), and DMAP (21 mg, 0.172 mmol) were added, and the reaction mixture was stirred at room temperature for 18 hours. The mixture was then concentrated under reduced pressure, the residue was diluted with DCM, and silica was added. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–40%) in hexanes to afford tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (113 mg, 70%) as a colorless oil. LCMS (System 1, Method D): m / z 470 / 472 (M+H) + (ES + ), 2.75 min, 190~320nm.

[0192] tert-Butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (114 mg, 0.243 mmol) and tert-butyl carbamate (28 mg, 0.2 To a degassed solution of CsCO (43 mmol), CsCO (198 mg, 0.606 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (22.2 mg, 0.024 mmol), and XPhos (CAS: 564483-18-7) (23.1 mg, 0.049 mmol) were added, and the reaction mixture was heated at 110 °C for 18 h. After cooling to room temperature, the mixture was filtered through a pad of Celite, and the residue was washed with EtOAc. The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel chromatography using a gradient of EtOAc (0-100%) in hexanes followed by a gradient of MeOH (0-30%) in DCM to afford tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (62 mg, 46%) as a pale yellow oil and tert-butyl (R)-2-amino-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (54 mg, 49%) as a yellow oil. LCMS (System 1, Method D): m / z 551 (M+H) + (ES + ), 2.11 min, 190~320nm. LCMS (System 1, Method D): m / z 451 (M+H) + (ES + ), 1.96 min, 190~320nm.

[0193] To a solution of tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (62 mg, 0.113 mmol) and tert-butyl (R)-2-amino-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (54 mg, 0.120 mmol) in DCM (1.10 mL) was added TFA (0.8 mL) and the reaction mixture was stirred at room temperature for 2 hours. The mixture was then concentrated to dryness, and the residue was purified using purification method A to give (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine, Example 1-1 (42 mg, 73%) as a white solid. Data for Example 1-1 are in Table 3.

[0194] Route B Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-3, Preparation of (R)-7-Ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine Dihydrochloride

[0195] [ka]

[0196] Under N2, a 100 mL flask was charged with tert-butyl (R)-(1-hydroxybutan-2-yl)carbamate (Intermediate 6) (2.55 g, 13.5 mmol), 2,4,6-trichloropyrimidin-5-ol (Intermediate 2) (1.50 g, 7.50 mmol), and anhydrous THF (30 mL). The resulting solution was cooled to 0 °C, and then Ph3P (3.15 g, 12.0 mmol) was added. Once Ph3P was completely dissolved, DIAD (2.36 mL, 12.0 mmol) was added dropwise over approximately 5 minutes. The reaction mixture was stirred at 0 °C for 10 minutes, then allowed to warm to room temperature and stirred for an additional 16 hours. The mixture was concentrated to dryness, and the residue was purified by column chromatography (dry load, 80 g SiO, 0:100 to 50:50, EtOAc:hexanes) to afford tert-butyl (R)-(1-((2,4,6-trichloropyrimidin-5-yl)oxy)butan-2-yl)carbamate (1.78 g, 64%) as a colorless solid. 1 H NMR (500 MHz, DMSO-d6) δ 0.89 (t, J = 7.4 Hz, 3H), 1.38 (s, 9H), 1.40 - 1.51 (m, 1H), 1.59 - 1.69 (m, 1H), 3.61 - 3.70 (m, 1H), 3.97 - 4.11 (m, 2H), 6.86 (d, J = 8.4 Hz, 1H).

[0197] A 100 mL flask was charged with tert-butyl (R)-(1-((2,4,6-trichloropyrimidin-5-yl)oxy)butan-2-yl)carbamate (1.78 g, 4.80 mmol) and DCM (20 mL). TFA (10.0 mL, 135 mmol) was then added, and the resulting solution was stirred at room temperature for 10 minutes. This mixture was co-evaporated with toluene under reduced pressure to give crude (R)-1-((2,4,6-trichloropyrimidin-5-yl)oxy)butan-2-amine trifluoroacetate as a colorless oil. The product was used in the next step without further purification. LCMS (System 1, Method E): m / z 234 / 236 (M-Cl) +(ES + ), 2.05 min, 190~320nm.

[0198] Under N2, a 20 mL sealable tube was charged with crude (R)-1-((2,4,6-trichloropyrimidin-5-yl)oxy)butan-2-amine trifluoroacetate (ca. 4.80 mmol) and 1,4-dioxane (13.5 mL). DIPEA (2.5 mL, 14.4 mmol) was then added, the tube was sealed, and the resulting solution was stirred at 80 °C for 16 h. The mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified by column chromatography (dry load, 40 g SiO2, 0:100 to 80:20, EtOAc:hexanes) to afford (R)-2,4-dichloro-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine (1.01 g, 90% overall over two steps) as a colorless oil. LCMS (System 1, Method D): m / z 234 / 236 (M+H) + (ES + ), 2.18 min, 190~320nm.

[0199] A 50 mL flask was charged with (R)-2,4-dichloro-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine (1.01 g, 4.31 mmol) and THF (21.5 mL). Next, DIPEA (1.5 mL, 8.6 mmol) was added, followed by di-tert-butyl dicarbonate (1.70 g, 7.77 mmol) and DMAP (53 mg, 0.43 mmol). The resulting solution was stirred at room temperature for 16 h. The mixture was evaporated under reduced pressure, and the residue was purified by column chromatography (dry load, 40 g SiO, 0:100 to 30:70, EtOAc:hexanes) to give tert-butyl (R)-2,4-dichloro-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (1.36 g, 94%) as a colorless solid. LCMS (System 1, Method D): m / z 278 / 280 (M-56+H) + (ES +), 2.68 min, 190~320nm.

[0200] Under N2, a 20 mL sealable tube was charged with tert-butyl (R)-2,4-dichloro-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (680 mg, 2.03 mmol), tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (448 mg, 2.24 mmol), and anhydrous 1,4-dioxane (5.8 mL). DIPEA (0.71 mL, 4.1 mmol) was added, the tube was then sealed, and the mixture was stirred at 80 °C for 16 h. The resulting mixture was cooled to room temperature and then diluted with DCM (30 mL) to dissolve and precipitate the solids. The resulting solution was concentrated under reduced pressure onto silica gel, and the residue was purified by column chromatography (dry load, 40 g SiO, 0:100 to 50:50, EtOAc:hexanes) to afford tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (878 mg, 87%) as a colorless solid. LCMS (System 1, Method D): m / z 442 / 444 (M-56+H) + (ES + ), 2.90 min, 190~320nm.

[0201] Under N2, a 20 mL sealable tube was charged with tert-butyl carbamate (310 mg, 2.64 mmol), Cs2CO3 (1.15 g, 3.53 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (80.7 mg, 0.0882 mmol), and XPhos (CAS: 564483-18-7) (168 mg, 0.353 mmol). Next, a solution of tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (878 mg, 1.76 mmol) in 1,4-dioxane (14 mL) was added, and the resulting suspension was sparged with N for 15 min. The tube was then sealed, and the mixture was stirred at 100 °C for 3 h. The mixture was concentrated under reduced pressure onto silica gel, and the residue was purified by column chromatography (dry pour, 40 g SiO, 0:100 to 60:10, EtOAc:hexanes) to give the still impure product as a yellow solid. The product was further purified by reverse-phase column chromatography (MeOH injection, 60 g C-18, 10:90 to 95:5, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to afford, after lyophilization, tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (630 mg, 62%) as a colorless solid. LCMS (System 1, Method D): m / z 579 (M+H) + (ES + ), 2.20 min, 190~320nm.

[0202] Under N2, a 50 mL flask was charged with tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-7-ethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (630 mg, 1.09 mmol) and 1,4-dioxane (5.5 mL). Once the solid dissolved, a solution of HCl in 1,4-dioxane (4 M, 5.5 mL, 22 mmol) was added, and the resulting mixture was stirred vigorously at 45 °C for 3 h. The resulting suspension was concentrated under reduced pressure, and the residue was dissolved in water (10 mL) and lyophilized to dryness. The resulting yellow solid was purified by reverse-phase column chromatography (HO injection, 60 g C-18, isocratic 5:95, MeCN:HO) to afford (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride, Example 1-3 (324 mg, 77%) as a colorless solid after lyophilization. Data for Examples 1-3 are in Table 3.

[0203] Route C Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-8, Preparation of 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine

[0204] [ka]

[0205] A 5 mL flask was charged with 4-((R)-3-aminopyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine (Example 2-3) (20 mg, 0.0657 mmol). Next, a solution of di-tert-butyl dicarbonate (15.1 mg, 0.0690 mmol) in THF (0.66 mL) was added, followed by a solution of NaHCO3 (11.6 mg) in water (0.33 mL). The resulting mixture was stirred at room temperature for 60 hours. The mixture was diluted with EtOAc (10 mL) and brine (5 mL), the phases were separated, and the aqueous phase was extracted with EtOAc (2 x 5 mL). The combined organic phases were dried over NaSO and concentrated to dryness to give tert-butyl ((3R)-1-(2-amino-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)carbamate (17.0 mg, 64%) as a colorless solid. This product was used in the next step without further purification. LCMS (System 1, Method D): m / z 405 (M+H) + (ES + ), 1.79 min, 190~320nm.

[0206] Under N2, a 10 mL flask was charged with tert-butyl ((3R)-1-(2-amino-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)carbamate (27.0 mg, 0.0668 mmol) and anhydrous THF (3.3 mL). The solution was cooled to 0 °C, and then a solution of LiAlH4 in THF (2 M, 0.17 mL, 0.34 mmol) was added dropwise. The resulting mixture was heated to reflux for 5 h, then cooled to 0 °C and carefully quenched by the addition of Na2SO4.10H2O (Danger: exotherm and H2 evolution). The resulting suspension was filtered, and the collected solid was washed several times with MeOH. The filtrate was filtered through a 0.45 μm filter and concentrated under reduced pressure. The residue was purified using purification method B to give 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine, Example 1-8 (14.0 mg, 66%) as a colorless solid. Data for Examples 1-8 are in Table 3.

[0207] Route D Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-13, Preparation of 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine

[0208] [ka]

[0209] Under N2, a 50 mL flask was charged with 2-(trimethylsilyl)ethyl (2-hydroxy-1-(oxetan-3-yl)ethyl)carbamate (Intermediate 20) (1.05 g, 4.01 mmol), 2,4,6-trichloropyrimidin-5-ol (Intermediate 2) (0.500 g, 2.51 mmol), and anhydrous THF (12.5 mL). The resulting solution was cooled to 0 °C, and then Ph3P (986 mg, 3.76 mmol) was added. Once Ph3P was completely dissolved, DIAD (0.740 mL, 3.76 mmol) was added dropwise over approximately 5 minutes. The reaction mixture was then stirred at 0 °C for 10 minutes, then warmed to room temperature, and stirred for an additional 16 hours. The mixture was concentrated to dryness, and the residue was purified by column chromatography (dry injection, 40 g SiO, 0:100 to 80:20, EtOAc:hexanes) to give the crude product, which was further purified by reverse-phase column chromatography (DMSO injection, 12 g C-18, 5:95 to 95:5, 0.1% formic acid in MeCN:water) to give 2-(trimethylsilyl)ethyl (1-(oxetan-3-yl)-2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)carbamate (847 mg, 63%). LCMS (System 1, Method D): m / z 414 / 416 (M-CO+H) + (ES + ), 2.20 min, 190~320nm.

[0210] Under N2, a 20 mL sealable tube was charged with 2-(trimethylsilyl)ethyl (1-(oxetan-3-yl)-2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)carbamate (690 mg, 1.28 mmol), tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (256 mg, 1.28 mmol), and anhydrous 1,4-dioxane (6.4 mL). DIPEA (0.45 mL, 2.58 mmol) was then added, the tube was sealed, and the mixture was stirred at 80 °C for 18 h. The resulting solution was concentrated to dryness, and the residue was purified by column chromatography (dry load, 40 g SiO, 0:100 to 90:10, EtOAc:hexanes) to afford tert-butyl ((3R)-1-(2,6-dichloro-5-(2-(oxetan-3-yl)-2-(((2-(trimethylsilyl)ethoxy)carbonyl)amino)ethoxy)pyrimidin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (730 mg, 94%) as a colorless solid. LCMS (System 1, Method D): m / z 578 / 580 (M-CO+H) + (ES + ), 2.32 min, 190~320nm.

[0211] Under N2, a 10 mL flask was charged with tert-butyl ((3R)-1-(2,6-dichloro-5-(2-(oxetan-3-yl)-2-(((2-(trimethylsilyl)ethoxy)carbonyl)amino)ethoxy)pyrimidin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (210 mg, 0.346 mmol) and anhydrous THF (3.5 mL). A solution of TBAF in THF (1 M, 0.865 mL, 0.865 mmol) was then added, and the resulting yellow solution was stirred at room temperature for 16 h. The reaction was quenched by the addition of aqueous saturated NH4Cl (1 mL) and water (1 mL). The resulting mixture was extracted with EtOAc (3 x 15 mL), and the combined organic phases were dried over MgSO4 and concentrated to dryness. The residue was purified by column chromatography (dry injection, 12 g SiO, 0:100 to 10:90, MeOH:DCM) to give the crude product, which was combined with the product from a similar reaction performed on approximately twice the scale and purified by reverse-phase column chromatography (MeOH injection, 12 g C-18, 5:95 to 70:30, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to give tert-butyl ((3R)-1-(5-(2-amino-2-(oxetan-3-yl)ethoxy)-2,6-dichloropyrimidin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (142 mg, 26%) as a colorless solid. LCMS (System 1, Method E): m / z 326 / 328 (M-Cl-BOC+H) + (ES + ), 1.97 min, 190~320nm.

[0212] Under N2, a 5 mL sealable tube was charged with tert-butyl ((3R)-1-(5-(2-amino-2-(oxetan-3-yl)ethoxy)-2,6-dichloropyrimidin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (142 mg, 0.307 mmol), Cs2CO3 (200 mg, 0.614 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (14.1 mg, 0.0154 mmol), and XPhos (CAS: 564483-18-7) (29.3 mg, 0.0614 mmol). 1,4-Dioxane (3.0 mL) was then added, and the resulting suspension was sparged with N2 for 15 min. The tube was then sealed, and the mixture was stirred for 3 h at 100° C. The mixture was concentrated under reduced pressure onto silica gel, and the residue was purified by column chromatography (dry load, 40 g SiO, 0:100 to 90:10, EtOAc:hexanes) to afford tert-butyl ((3R)-1-(2-chloro-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (70 mg, 54%) as a colorless solid. LCMS (System 1, Method D): m / z 426 / 428 (M+H) + (ES + ), 2.00 min, 190~320nm.

[0213] Under N2, a 5 mL sealable tube was charged with tert-butyl carbamate (28.9 mg, 0.247 mmol), Cs2CO3 (107 mg, 0.329 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (15.1 mg, 0.0164 mmol), and XPhos (CAS: 564483-18-7) (31.3 mg, 0.0657 mmol). A solution of tert-butyl ((3R)-1-(2-chloro-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (70.0 mg, 0.164 mmol) in 1,4-dioxane (1.6 mL) was then added, and the resulting suspension was sparged with N for 15 min. The tube was then sealed, and the mixture was stirred at 110 °C for 11 h. The mixture was concentrated under reduced pressure onto silica gel, and the residue was purified by reverse-phase column chromatography (DMSO injection, 30 g C-18, 5:95 to 90:10, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to give tert-butyl((3R)-1-(2-((tert-butoxycarbonyl)amino)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4- b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (15 mg, 18%) as a colorless solid, and tert-butyl ((3R)-1-(2-amino-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (15 mg, 22%) as a colorless solid. LCMS (System 1, Method D): m / z 507 (M+H) + (ES + ), 2.06 min, 190~320nm. LCMS (System 1, Method D): m / z 407 (M+H) + (ES + ), 1.83 min, 190~320nm.

[0214] A 5 mL flask was charged with a mixture of tert-butyl ((3R)-1-(2-((tert-butoxycarbonyl)amino)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate and tert-butyl ((3R)-1-(2-amino-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (total 0.0620 mmol) and DCM (1.0 mL) under N. TFA (0.2 mL) was then added, and the resulting solution was stirred at room temperature for 5 h. The solution was co-evaporated under reduced pressure with toluene, and the residue was purified using purification method C to give, after lyophilization, 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine, Example 1-13 (13.6 mg, 72%) as a colorless solid. Data for Examples 1-13 are in Table 3.

[0215] Route E Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-14, Preparation of (R)-7,7-Dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine Dihydrochloride

[0216] [ka]

[0217] To a solution of 2,4,6-trichloro-5-methoxypyrimidine (Intermediate 1) (1.00 g, 4.68 mmol) in 1,4-dioxane (7.00 mL) were added 2-amino-2-methylpropan-1-ol (Intermediate 21) (0.47 mL, 4.92 mmol) and DIPEA (2.00 mL, 11.7 mmol), and the reaction mixture was heated at 100° C. for 3 hours. After cooling to room temperature, the mixture was diluted with water, and the aqueous layer was extracted with EtOAc (3×20 mL). The combined organic extracts were washed with brine, dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–80%) in hexanes to afford 2-((2,6-dichloro-5-methoxypyrimidin-4-yl)amino)-2-methylpropan-1-ol (891 mg, 72%) as a colorless oil that crystallized to a white solid on standing. LCMS (System 1, Method E): m / z 266 / 268 (M+H) + (ES + ), 2.05 min, 190~320nm.

[0218] To a solution of 2-((2,6-dichloro-5-methoxypyrimidin-4-yl)amino)-2-methylpropan-1-ol (800 mg, 3.01 mmol) in DMA (6.0 mL) was added LiCl (318 mg, 7.51 mmol), and the mixture was stirred for 20 minutes at 160° C. under microwave heating. After cooling to room temperature, the mixture was directly purified by reverse-phase column chromatography (C-18, isocratic 5:95, 0.1% formic acid in MeCN:water) to give 2,4-dichloro-6-((1-hydroxy-2-methylpropan-2-yl)amino)pyrimidin-5-ol formate (130 mg, 17%) as a brown oil. LCMS (System 1, Method D): m / z 252 / 254 (M+H) + (ES + ), 1.99 min, 190~320nm.

[0219] To a solution of 2,4-dichloro-6-((1-hydroxy-2-methylpropan-2-yl)amino)pyrimidin-5-ol formate (200 mg, 0.793 mmol) in THF (4.7 mL) cooled to 0° C., Ph3P (416 mg, 1.59 mmol) and DIAD (0.313 mL, 1.59 mmol) were added, and the reaction mixture was stirred at room temperature overnight. The mixture was then concentrated to dryness to give crude 2,4-dichloro-7,7-dimethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine, which was used directly in the next step without any purification. LCMS (System 1, Method D): m / z 234 / 236 (M+H) + (ES + ), 1.87 min, 190~320nm.

[0220] To a solution of crude 2,4-dichloro-7,7-dimethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazine (0.793 mmol) in THF (7.9 mL) was added di-tert-butyl dicarbonate (537 mg, 2.46 mmol), EtN (0.33 mL, 2.46 mmol), and DMAP (48 mg, 0.396 mmol), and the reaction mixture was stirred at room temperature for 18 h. The mixture was then concentrated under reduced pressure and silica was added. The residue was purified by silica gel chromatography (dry-packed) using a gradient of 0–50% EtOAc in hexanes to afford tert-butyl 2,4-dichloro-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (30 mg, 11% overall over two steps) as a colorless oil. LCMS (System 1, Method D): m / z 278 / 280 (M-56+H) + (ES + ), 2.26 min, 190~320nm.

[0221] To a solution of tert-butyl 2,4-dichloro-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (30 mg, 0.089 mmol) in 1,4-dioxane (0.3 mL) was added tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (20 mg, 0.099 mmol) and DIPEA (0.031 mL, 0.18 mmol), and the mixture was heated to 80° C. for 18 hours. After cooling to room temperature, the mixture was diluted with water, and the aqueous layer was extracted with EtOAc (3×10.0 mL). The combined organic extracts were dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–60%) in hexanes to afford tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (37 mg, 83%) as a colorless oil. LCMS (System 1, Method D): m / z 442 / 444 (M-56+H) + (ES + ), 2.41 min, 190~320nm.

[0222] tert-Butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (37 mg, 0.074 mmol) and tert-butyl carbamate (13 mg To a degassed solution of CsCO (61 mg, 0.186 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (6.8 mg, 0.00743 mmol), and XPhos (CAS: 564483-18-7) (7.1 mg, 0.0149 mmol) were added, and the reaction mixture was heated to 110 °C for 2 h. After cooling to room temperature, the mixture was filtered through a pad of Celite, and the residue was washed with EtOAc. The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel chromatography using a gradient of EtOAc (0–100%) in hexanes, followed by reverse-phase column chromatography (C-18, isocratic 5:95, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to afford tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (22 mg, 51%) as a colorless oil. LCMS (System 1, Method D): m / z 579 (M+H) + (ES + ), 2.29 min, 190~320nm.

[0223] To a solution of tert-butyl (R)-4-(3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-((tert-butoxycarbonyl)amino)-7,7-dimethyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (22 mg, 0.038 mmol) in 1,4-dioxane (0.48 mL) was added a solution of HCl in 1,4-dioxane (4 M, 0.48 mL, 1.9 mmol) and the reaction mixture was heated to 45° C. for 3 h. After cooling to room temperature, the mixture was concentrated to dryness. The residue was diluted with water and purified by reverse-phase column chromatography (HO injection, 4 g of C-18, isocratic 5:95, MeCN:HO) to afford (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine dihydrochloride, Example 1-14 (12 mg, 90%) as a white solid. Data for Examples 1-14 are in Table 3.

[0224] Route F Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-20, Preparation of 7-Isopropyl-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine

[0225] [ka]

[0226] To a solution of tert-butyl ((3R)-1-(2-chloro-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (Intermediate 30) (100 mg, 0.243 mmol) in DMF (1.30 mL) cooled to 0° C., NaH (60% dispersion in mineral oil, 15 mg, 0.364 mmol) and iodomethane (0.018 mL, 0.291 mmol) were added, and the reaction mixture was stirred at 0° C. for 30 minutes, then warmed to room temperature and stirred for an additional hour. The mixture was then quenched with water, and the aqueous layer was extracted with EtOAc (3×5.00 mL). The combined organic extracts were dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–40%) in hexanes to afford tert-butyl ((3R)-1-(2-chloro-7-isopropyl-8-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (68 mg, 66%) as a pale yellow oil. LCMS (System 1, Method D): m / z 426 / 428 (M+H) + (ES + ), 2.83 min, 190~320nm.

[0227] To a degassed solution of tert-butyl ((3R)-1-(2-chloro-7-isopropyl-8-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (68 mg, 0.16 mmol) and tert-butyl carbamate (19 mg, 0.16 mmol) in 1,4-dioxane (1.60 mL) was added CsCO (130 mg, 0.399 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (14.6 mg, 0.016 mmol) and XPhos (CAS: 564483-18-7) (15.2 mg, 0.032 mmol), and the reaction mixture was heated at 110 °C for 18 h. The mixture was cooled to room temperature, filtered through a pad of Celite, and the residue was washed with EtOAc. The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel chromatography using a gradient of 0-50% EtOAc in hexanes to give tert-butyl ((3R)-1-(2-((tert-butoxycarbonyl)amino)-7-isopropyl-8-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (43 mg, 53%) as a pale yellow oil. LCMS (System 1, Method D): m / z 507 (M+H) + (ES + ), 2.17 min, 190~320nm.

[0228] To a solution of tert-butyl ((3R)-1-(2-((tert-butoxycarbonyl)amino)-7-isopropyl-8-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (43 mg, 0.085 mmol) in DCM (0.50 mL) was added TFA (0.50 mL), and the reaction mixture was stirred at room temperature for 2 hours. The mixture was then concentrated to dryness, and the residue was purified by purification method D to afford 7-isopropyl-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine, Example 1-20 (12 mg, 46%) as a white solid. Data for Examples 1-20 are in Table 3.

[0229] Route G Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 1-21, Preparation of 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine

[0230] [ka]

[0231] To a solution of 2,4,6-trichloro-5-methoxypyrimidine (Intermediate 1) (320 mg, 1.5 mmol) in ethanol (10 mL), pyrrolidin-2-ylmethanol (Intermediate 31) (227 mg, 2.25 mmol) and TEA (0.42 mL, 3.0 mmol) were added, and the reaction mixture was stirred at room temperature for 1 hour. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in DCM (10 mL). The DCM solution was washed with water (10 mL), dried over Na2SO4, and concentrated under reduced pressure. The residue was purified using flash chromatography with 0 to 60% ethyl acetate in hexane to afford (1-(2,6-dichloro-5-methoxypyrimidin-4-yl)pyrrolidin-2-yl)methanol (400 mg, 96%) as a white solid. LCMS (System 1, Method D): m / z 278 / 280 (M+H) + (ES + ), 2.13 min, 190~320nm.

[0232] To a solution of (1-(2,6-dichloro-5-methoxypyrimidin-4-yl)pyrrolidin-2-yl)methanol (400 mg, 1.44 mmol) in DMF (5 mL) was added LiCl (152 mg, 3.6 mmol), and the mixture was stirred for 20 min at 160° C. under microwave heating. The reaction mixture was concentrated, and the residue was purified by flash chromatography using 0 to 100% ethyl acetate in hexanes to give 2,4-dichloro-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazine (150 mg, 42%) as a white solid and 2,4-dichloro-6-(2-(hydroxymethyl)pyrrolidin-1-yl)pyrimidin-5-ol (100 mg, 26%) as a white solid. 2,4-Dichloro-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazine: 1H NMR (500 MHz, chloroform-d) δ 1.48–1.58 (m, 1H), 1.99–2.10 (m, 1H), 2.14–2.19 (m, 1H), 2.20–2.26 (m, 1H), 3.44–3.50 (m, 1H), 3.56–3.64 (m, 1H), 3.72–3.80 (m, 2H), 4.62–4.67 (m, 1H). LCMS (System 1, Method D): m / z 246 / 248 (M+H) + (ES + ), 2.20 minutes, 190-320 nm. 2,4-Dichloro-6-(2-(hydroxymethyl)pyrrolidin-1-yl)pyrimidin-5-ol: LCMS (System 1, Method D): m / z 264 / 266 (M+H) + (ES + ), 1.97 min, 190~320nm.

[0233] To a solution of 2,4-dichloro-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazine (200 mg, 0.813 mmol) and tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (0.163 g, 0.813 mmol) in 1,4-dioxane (4 mL) was added DIPEA (0.28 mL, 1.63 mmol), and the resulting mixture was stirred at 80° C. for 24 hours. The reaction mixture was concentrated, and the residue was purified by flash chromatography using 0 to 60% ethyl acetate in hexanes to afford tert-butyl ((3R)-1-(2-chloro-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (252 mg, 75%) as a white solid. LCMS (System 1, Method D): m / z 410 / 412 (M+H) + (ES + ), 2.64 min, 190~320nm.

[0234] To a degassed solution of tert-butyl ((3R)-1-(2-chloro-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (150 mg, 0.366 mmol) and tert-butyl carbamate (42.9 mg, 0.366 mmol) in 1,4-dioxane (4 mL) was added CsCO (0.477 g, 1.46 mol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (21.0 mg, 0.0365 mmol), and XPhos (CAS: 564483-18-7) (34.9 mg, 0.0732 mmol). The solution was degassed with N and stirred at 80° C. for 18 hours. A mixture of tert-butyl ((3R)-1-(2-((tert-butoxycarbonyl)amino)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate and tert-butyl ((3R)-1-(2-amino-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate was observed by LCMS. The reaction mixture was filtered under vacuum over a bed of Celite, the residue was washed with ethyl acetate, and the filtrate was concentrated to give a mixture of two products as a yellow oil, which was used directly in the next step without any purification. LCMS (System 1, Method D): m / z 491 (M+H) + (ES + ), 2.11 min, 190~320nm. LCMS (System 1, Method D): m / z 391 (M+H) + (ES + ), 1.91 min, 190~320nm.

[0235] The yellow oil from the above step was dissolved in DCM (2.0 mL) and TFA (2.0 mL) was added at room temperature. The mixture was stirred at room temperature for 1 hour and then concentrated to dryness. The residue was purified using reverse-phase column chromatography (C-18, isocratic 5:95, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to afford 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine, Example 1-21 (21 mg, 20% over two steps) as a white solid. Data for Examples 1-21 are in Table 3.

[0236] Route H Typical Procedure for the Preparation of Fused Pyrimidines, Illustrated by Example 7-1, Preparation of (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine

[0237] [ka]

[0238] A 25 mL flask was charged with 20% Pd(OH) / C (15.1 mg, 10 mol% Pd). The atmosphere was replaced with N, and then a solution of tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7-isopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (Intermediate 41) (110 mg, 0.215 mmol) in EtOH (2.1 mL) was added, followed by ammonium formate (135 mg, 2.15 mmol). The reaction mixture was heated to reflux for 3 h. After cooling to room temperature, the reaction mixture was filtered through a pad of Celite, and the residue was washed several times with MeOH. The filtrate was concentrated under reduced pressure to afford tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-7-isopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate as a white solid (103 mg, 100%), which was used directly in the next step without further purification. LCMS (System 1, Method D): m / z 478 (M+H) + (ES + ), 2.22 min, 190~320nm.

[0239] To a solution of tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-7-isopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (103 mg, 0.216 mmol) in DCM (1.00 mL) was added TFA (1.00 mL), and the reaction mixture was stirred at room temperature for 2 hours. The mixture was then concentrated to dryness, and the residue was purified by purification method E to afford (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine, Example 7-1 (43 mg, 72%), as a white foam. Data for Example 7-1 are in Table 3.

[0240] Route I Typical Procedure for the Preparation of Fused Pyrimidines, Illustrated by Example 7-2, Preparation of (R)-1-((R)-7-cyclopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine

[0241] [ka]

[0242] To tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2-chloro-7-cyclopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (Intermediate 42) (270 mg, 0.530 mmol) in MeOH (20 mL) was added 10% Pd / C catalyst (50 mg), and the resulting mixture was stirred under H (1 atmosphere pressure) for 8 h at room temperature. The reaction mixture was then filtered over a bed of Celite, and the residue was washed with MeOH. The filtrate was concentrated under reduced pressure to give tert-butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-7-cyclopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (250 mg, 99%) as a brown solid, which was carried on to the next step without purification. LCMS (System 4, Method C): m / z 476 (M+H) + (ES + ), 4.84 minutes, 228nm

[0243] tert-Butyl (R)-4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-7-cyclopropyl-6,7-dihydro-8H-pyrimido[5,4-b][1,4]oxazine-8-carboxylate (250 mg, 0.526 mmol) was dissolved in DCM (5.00 mL), and TFA (5 mL) was added dropwise at 0° C., and the resulting mixture was stirred at room temperature for 2 hours. The solvent was removed in vacuo and the residue was triturated with hexanes (2 × 20 mL) and diethyl ether (2 × 10 mL) to give the crude product (150 mg), which was purified by purification method F to give (R)-1-((R)-7-cyclopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine, Example 7-2 (35 mg, 22%) as a white solid. Data for Example 7-2 are in Table 3.

[0244] Route J Typical Procedure for the Preparation of Fused Pyrimidines, Illustrated by Example 10-1, Preparation of (3R)-1-(7-Isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine

[0245] [ka]

[0246] A vial was charged with tert-butyl ((3R)-1-(2-chloro-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (Intermediate 30) (110 mg, 0.267 mmol), trimethylboroxine (CAS: 823-96-1) (0.15 mL, 1.07 mmol), KCO (74 mg, 0.534 mmol), and DME (5.80 mL). Next, [1,1'-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (CAS: 72287-26-4) (20 mg, 0.027 mmol) was added, and the mixture was degassed with nitrogen for 15 minutes. The tube was sealed, and the mixture was heated at 100 °C for 18 hours. After cooling to room temperature, the mixture was filtered through a pad of Celite, and the residue was washed with EtOAc. The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel chromatography using a gradient of 0 to 100% EtOAc in hexanes to afford tert-butyl ((3R)-1-(7-isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (69 mg, 66%) as a colorless oil. LCMS (System 1, Method D): m / z 392 (M+H) + (ES + ), 2.02 min, 190~320nm.

[0247] To a solution of tert-butyl ((3R)-1-(7-isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (61 mg, 0.156 mmol) in DCM (1.00 mL) was added TFA (1.00 mL), and the reaction mixture was stirred at room temperature for 1 hour. After concentration to dryness, the residue was purified by purification method D to afford (3R)-1-(7-isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine, Example 10-1 (35 mg, 77%) as a white solid. Data for Example 10-1 are in Table 3.

[0248] Route K Typical Procedures for the Preparation of Fused Pyrimidines, Illustrated by Examples 11-10, Preparation of (S)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-2-amine

[0249] [ka]

[0250] To a solution of 2,4,6-trichloropyrimidin-5-ol (Intermediate 2) (550 mg, 2.76 mmol) and tert-butyl (S)-2-(2-hydroxyethyl)pyrrolidine-1-carboxylate (Intermediate 66) (1.19 g, 5.52 mmol) in THF (15.5 mL) cooled to 0° C., Ph3P (1.09 g, 4.14 mmol) and DIAD (0.815 mL, 4.14 mmol) were added, and the reaction mixture was stirred at room temperature for 3 hours. The THF was then removed by concentration under reduced pressure, and silica was added. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–50%) in hexanes to afford tert-butyl (S)-2-(2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)pyrrolidine-1-carboxylate (844 mg, 77%) as a colorless oil. 1 H NMR (400 MHz, chloroform-d) δ 1.45 (s, 9H), 1.75–1.83 (m, 1H), 1.83–1.97 (m, 3H), 1.99–2.11 (m, 1H), 2.25–2.36 (m, 1H), 3.26–3.48 (m, 2H), 3.95–4.06 (m, 1H), 4.07–4.23 (m, 2H).

[0251] To a solution of tert-butyl (S)-2-(2-((2,4,6-trichloropyrimidin-5-yl)oxy)ethyl)pyrrolidine-1-carboxylate (820 mg, 2.07 mmol) and tert-butyl (R)-methyl(pyrrolidin-3-yl)carbamate (Intermediate 4) (414 mg, 2.07 mmol) in 1,4-dioxane (4.73 mL) was added DIPEA (1.08 mL, 6.2 mmol), and the reaction mixture was heated at 80° C. for 3 h. The mixture was then diluted with water, and the aqueous layer was extracted with EtOAc (×3). The combined organic extracts were washed with brine, dried (MgSO), filtered, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (dry packing) using a gradient of EtOAc (0–80%) in hexanes to afford tert-butyl (S)-2-(2-((4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2,6-dichloropyrimidin-5-yl)oxy)ethyl)pyrrolidine-1-carboxylate (1.07 g, 92%) as a colorless oil. LCMS (System 1, Method D): m / z 560 / 562 (M+H) + (ES + ), 2.39 min, 190~320nm.

[0252] To a solution of tert-butyl (S)-2-(2-((4-((R)-3-((tert-butoxycarbonyl)(methyl)amino)pyrrolidin-1-yl)-2,6-dichloropyrimidin-5-yl)oxy)ethyl)pyrrolidine-1-carboxylate (1.07 g, 1.91 mmol) in DCM (8.00 mL) was added TFA (8.00 mL), and the reaction mixture was stirred at room temperature for 10 minutes. The mixture was then concentrated to dryness to give crude (R)-1-(2,6-dichloro-5-(2-((S)-pyrrolidin-2-yl)ethoxy)pyrimidin-4-yl)-N-methylpyrrolidin-3-amine ditrifluoroacetate as a yellow oil, which was used directly in the next step without any purification. LCMS (System 1, Method D): m / z 360 / 362 (M+H) + (ES+ ), 1.35 min, 190~320nm.

[0253] To a solution of crude (R)-1-(2,6-dichloro-5-(2-((S)-pyrrolidin-2-yl)ethoxy)pyrimidin-4-yl)-N-methylpyrrolidin-3-amine ditrifluoroacetate (1.91 mmol) in 1,4-dioxane (5.1 mL) was added DIPEA (1.33 mL, 7.64 mmol) and the reaction mixture was heated at 80° C. for 18 h. The mixture was then concentrated under reduced pressure, and the residue was purified by silica gel chromatography (dry loading) using a gradient of MeOH (0–30%) in DCM to afford (R)-1-((S)-2-chloro-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)-N-methylpyrrolidin-3-amine (620 mg, 100%) as a beige solid. LCMS (System 1, Method D): m / z 324 / 326 (M+H) + (ES + ), 1.86 min, 190~320nm.

[0254] To a solution of (R)-1-((S)-2-chloro-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)-N-methylpyrrolidin-3-amine (618 mg, 1.91 mmol) in THF (19.1 mL) was added di-tert-butyl dicarbonate (833 mg, 3.82 mmol), EtN (0.567 mL, 4.2 mmol), and DMAP (117 mg, 0.954 mmol), and the reaction mixture was stirred at room temperature for 18 hours. The mixture was then concentrated under reduced pressure, and silica was added. The residue was purified by silica gel chromatography (dry-packed) using a gradient of EtOAc (0–70%) in hexanes to afford tert-butyl ((R)-1-((S)-2-chloro-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (480 mg, 59%) as a colorless oil. LCMS (System 1, Method D): m / z 424 / 426 (M+H) + (ES + ), 2.27 min, 190~320nm.

[0255] tert-Butyl ((R)-1-((S)-2-chloro-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (480 mg, 1.13 mmol) and tert-butyl carbamate (199 mg) in 1,4-dioxane (11.3 mL) To a degassed solution of CsCO (922 mg, 2.83 mmol), tris(dibenzylideneacetone)dipalladium(0) (CAS: 51364-51-3) (104 mg, 0.113 mmol), and XPhos (CAS: 564483-18-7) (216 mg, 0.453 mmol) were added, and the reaction mixture was heated at 110 °C for 3 h. After cooling to room temperature, the mixture was filtered through a pad of Celite, and the residue was washed with EtOAc. The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel chromatography using a gradient of EtOAc (0–100%) in hexanes, followed by reverse-phase column chromatography (C-18, isocratic 5:95, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to afford tert-butyl ((R)-1-((S)-2-((tert-butoxycarbonyl)amino)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (291 mg, 51%) as a beige solid. LCMS (System 2, Method F): m / z 505 (M+H) + (ES + ), 2.15 min, 254 nm.

[0256] To a solution of tert-butyl ((R)-1-((S)-2-((tert-butoxycarbonyl)amino)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-4-yl)pyrrolidin-3-yl)(methyl)carbamate (291 mg, 0.577 mmol) in 1,4-dioxane (5.77 mL) was added a solution of HCl in 1,4-dioxane (4 M, 5.77 mL, 23.1 mmol), and the reaction mixture was heated at 45° C. for 3 hours. The mixture was cooled to room temperature and then concentrated to dryness. The residue was diluted with water and purified by reverse-phase column chromatography (C-18, isocratic 5:95, MeCN:HO) and then repurified by reverse-phase column chromatography (C-18, isocratic 5:95, 10 mM ammonium bicarbonate in MeCN:water, pH 10) to afford (S)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxoazepin-2-amine, Example 11-10 (85 mg, 48%) as a white solid. Data for Examples 11-10 are in Table 3.

[0257] [Table 4-1]

[0258] [Table 4-2]

[0259] [Table 4-3]

[0260] [Table 4-4]

[0261] [Table 4-5]

[0262] Table 4-6

[0263] Table 5-1

[0264] Table 5-2

[0265] Table 5-3

[0266] Table 5-4

[0267] Table 5-5

[0268] Table 5-6

[0269] Table 5-7

[0270] Table 5-8

[0271] Table 5-9

[0272] Table 5-10

[0273] Table 5-11

[0274] Table 5-12

[0275] Table 5-13

[0276] Table 5-14

[0277] Table 5-15

[0278] Table 5-16

[0279] Table 5-17

[0280] Table 5-18

[0281] Table 5-19

[0282] [Table 5-20]

[0283] biological activity H4 antagonist functional cAMP Gi assay HEKf cells were infected overnight using a baculovirus expressing the human H4 receptor, then centrifuged at 1,200 rpm for 5 minutes, frozen in cell freezing medium (Sigma), and stored at -150°C. On the day of the assay, cells were thawed and resuspended in HBSS containing 500 nM IBMX to achieve a density of 1,500 cells / well. H4 ligand was prepared in DMSO and stamped into low-volume plates at 25 nL using a LabCyte ECHO acoustic dispenser. Cells were plated at 10 μL / well in the presence of 1 μM forskolin, centrifuged at 1,200 rpm for 1 minute, and incubated for 30 minutes before adding Cisbio cAMP detection reagent to a total volume of 20 μL / well. For antagonist assays, cells were preincubated with the H4 antagonist ligand for 30 minutes, followed by EC 80 Histamine was added at a concentration of 0.05% and incubated for an additional 30 minutes. After adding detection reagent and shaking for 60 minutes at room temperature, cAMP accumulation was measured using HTRF on a PheraStar plate reader. EC 50 Values ​​were generated using a four-parameter logistic fit equation to quantify agonist potency. Functional agonist affinity values ​​were generated using the Cheng-Prusoff equation, and antagonist assay data were used to calculate pK b The value of was calculated.

[0284] H4 antagonist functional dynamic mass redistribution assay HEKf cells were infected with a baculovirus expressing the human H4 receptor and plated onto fibronectin-coated EPIC plates at a density of 10,000 cells / well and incubated overnight at 37°C. The cell medium was changed to 30 μL HBSS containing 20 mM HEPES per well, and 30 nL DMSO was added per well using a LabCyte ECHO acoustic dispenser. After 2 hours of equilibration at room temperature, 30 nL of H4 ligand prepared in DMSO was stamped onto the seeded EPIC plates using a LabCyte ECHO acoustic dispenser, and dynamic cell mass redistribution was monitored using a Corning EPIC plate reader. After 45 minutes of measurement, 30 nL / well of histamine EC4 was added. 80 Antagonist assay data was obtained by adding and monitoring EC values. The maximum baseline-corrected response (pm) was used to generate concentration-response curves. EC 50 Values ​​were generated using a four-parameter logistic fit equation to quantify agonist potency. Functional agonist affinity values ​​were generated using the Cheng-Prusoff equation, and antagonist assay data were used to calculate pK b values ​​were calculated.

[0285] hERG assay hERG assay data were determined by Metrion Biosciences, Cambridge (UK) using the experimental protocol detailed below.

[0286] A Chinese hamster ovary (CHO) cell line stably expressing the human ether-a-go-go related gene was grown and passaged under standard culture conditions. Cells were prepared for the assay using a dissociation protocol designed to optimize cell health, yield, and sealing and assay quality. Test samples were obtained as 10 mM stock solutions in 100% DMSO. All sample handling and serial dilutions were performed using glass vessels and glass-lined plates. A maximum working concentration of 30 μM was prepared from the 10 mM sample stock solution using a 1.333-fold dilution into the external recording solution (0.3% DMSO v / v). For single-concentration assays, test samples were screened at 30 μM on a minimum of three individual cells. pIC 50 In the assay, test samples were screened at 1, 3, 10, and 30 μM on a minimum of three individual cells. Each concentration of sample was added cumulatively twice to the same cell to construct each four-point concentration-response curve.

[0287] All experiments were performed on a QPatch gigaseal automated patch clamp platform. The compositions of the external and internal recording solutions for QPatch experiments are shown below in Table A. All solutions were filtered (0.2 μm) before each experiment.

[0288] [Table 6]

[0289] All recordings were performed in conventional whole-cell configuration at room temperature (approximately 21°C) using standard single-hole tips (Rchip 1.5-4 MΩ). A series of resistance values ​​(4-15 MΩ) were compensated for >80%. Currents were generated from a holding potential of -90 mV using the industry-standard "+40 / -40" voltage protocol, as shown in Figure A below. This was applied at a stimulation frequency of 0.1 Hz.

[0290] [ka]

[0291] Once the whole-cell configuration was achieved, vehicle (0.3% DMSO v / v in the external recording solution) was applied to each cell in two bolus additions, with a 2-minute recording period between each addition to allow for stable recording. After the vehicle phase, either: i) For single concentration assays - a single concentration of test sample was applied at 30 μM as five bolus additions per test concentration, spaced 2 minutes apart; or ii) pIC 50 For the assay - four concentrations of test sample were applied at 2 min intervals from 1 μM to 30 μM as two bolus additions per test concentration; The effect on hERG tail current amplitude was then measured during a 4-minute recording period. For each sweep of the voltage protocol, membrane currents and passive properties of individual cells were recorded using QPatch assay software (version 5.0). The peak outward tail current amplitude generated during the test pulse to -40 mV was measured relative to the instantaneous leak current measured during the initial prepulse step to -40 mV. For QC purposes, the minimum current amplitude for this assay is a peak outward current of >200 pA measured at the end of the vehicle phase. The QPatch analysis software calculated the average peak current for the last three sweeps at the end of each concentration application period, exported the data to Excel, and examined using a bioinformatics suite developed to run in Pipeline Pilot (Biovia, USA). This template calculates the percent inhibition for each test concentration application period as the reduction in average peak current or charge number relative to the value measured at the end of the control (i.e., vehicle) period. Percent inhibition values ​​from each cell were used to construct concentration-response curves using a four-parameter logistic fit with 0% and 100% inhibition levels and a free Hill slope coefficient, fixed at very low and very high concentrations, respectively. The IC 50 Calculate the 50% inhibitory concentration (IC) and Hill coefficient, but include only data from cells where the Hill slope is within the range 0.5 > nH < 2.0. The IC reported below 50Data represent the average of at least three individual cells (N≧3). By convention, test samples that fail to achieve >40% inhibition at the highest concentration are given an equivocal IC due to poor or unconstrained fitness. 50 In this case, any IC value that is 0.5 log units higher than the highest concentration tested is used. 50 For example, if a sample fails to demonstrate a mean inhibition of >40% at the highest concentration of 30 μM, an IC value of 100 μM was returned. 50 value, i.e., pIC 50 Report ≦4.0.

[0292] The majority of the examples are prepared as single enantiomers or single diastereomers. However, some compounds are prepared as racemates or mixtures of diastereomers, and sometimes these racemates or diastereomers are separated into single isomers using reverse-phase HPLC, chiral HPLC, or chiral SFC techniques. For these particular compounds, the isomer assignment (isomer 1, isomer 2) is based on the retention time of the compound using the separation technique used in the final isomer separation step. By implication, this can be the retention time of reverse-phase HPLC, chiral HPLC, or chiral SFC, which will vary from compound to compound.

[0293] [Table 7-1]

[0294] [Table 7-2]

Claims

1. Compound of formula (1): 【Chemistry 1】 or its salts (In the formula, Z is H, NH ２ or C １～３ is alkyl, Y is, 【Chemistry 2】 is selected from the group consisting of n is 0 or 1; R １ is H or C optionally substituted with 1 to 6 fluorine atoms １～３ alkyl or R ３ to form a ring optionally substituted with 1 to 6 fluorine atoms, R ２ is H, optionally substituted C １～６ Alkyl, optionally substituted C ３～６ cycloalkyl or an optionally substituted 3- to 6-membered heterocyclyl group, the optional substituents being OC １～３ alkyl or 1 to 6 fluorine atoms, or R ２ is R ３ to form a ring optionally substituted with 1 to 6 fluorine atoms, R ３ is H or C optionally substituted with 1 to 6 fluorine atoms １～３ alkyl or R １ to form a ring optionally substituted with 1 to 6 fluorine atoms, or R ２ to form a ring optionally substituted with 1 to 6 fluorine atoms, or R ４ to form a ring optionally substituted with 1 to 6 fluorine atoms, R ４ is H or C optionally substituted with 1 to 6 fluorine atoms １～３ alkyl or R ３ to form a ring optionally substituted with 1 to 6 fluorine atoms, or R ５ to form a ring optionally substituted with 1 to 6 fluorine atoms, R ５ is H or C optionally substituted with 1 to 6 fluorine atoms １～３ alkyl or R ４ to form a ring optionally substituted with 1 to 6 fluorine atoms, R ６ is H or methyl).

2. Formula (2a) or (2b): 【Transformation 3】 2. The compound according to claim 1, wherein:

3. Formula (3a) or (3b): 【Chemistry 4】 3. The compound according to claim 2, wherein:

4. Formula (2c) or (2d): 【Transformation 5】 2. The compound according to claim 1, wherein:

5. Formula (2e): 【Transformation 6】 2. The compound according to claim 1, wherein:

6. Formula (3c): 【Transformation 7】 6. The compound according to claim 5, wherein:

7. Formula (4): 【Transformation 8】 2. The compound according to claim 1, wherein:

8. Z is H, NH ２ or methyl.

9. Z is NH ２ 9. The compound of claim 8, wherein:

10. R １ 10. The compound of claim 1, wherein is H.

11. R ２ is H, methyl, ethyl, isopropyl, cyclopropyl, isobutyl, trifluoromethyl, CH ２ OMe, CH(CH ３ ) OMe, C(CH ３ ) ２ 11. The compound of any one of claims 1 to 10, selected from the group consisting of OMe and oxetanyl.

12. R ２ The compound of claim 11 , wherein is ethyl or isopropyl.

13. R ３ 13. The compound of any one of claims 1 to 12, wherein is H or methyl.

14. R ２ is ethyl, and R ３ 12. The compound of any one of claims 1 to 11, wherein is methyl.

15. R ４ 15. The compound of any one of claims 1 to 14, wherein is H, methyl, ethyl or isopropyl.

16. R ４ The compound of claim 15, wherein is H.

17. 16. The compound of any one of claims 1 to 15, wherein n is 0.

18. 16. The compound of any one of claims 1 to 15, wherein n is 1.

19. (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-cyclopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-isobutyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-(methoxymethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((S)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(oxetan-3-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 6-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6S,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6R,7R)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6S,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (6R,7S)-6,7-dimethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 7-Isopropyl-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-6a-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (S)-6a-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-aminopyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-((S)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-7-(2-methoxypropan-2-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-cyclopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((S)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-(3-aminoazetidin-1-yl)-7-ethyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-4-(3-aminoazetidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine (R)-7-isopropyl-4-(4-methylpiperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-(piperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((R)-7-cyclopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (3R)-1-(7-(methoxymethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (3R)-N-methyl-1-(6-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-((S)-7-((R)-1-methoxyethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-1-(7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylazetidin-3-amine; (3R)-1-(7-isopropyl-2-methyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)-N-methylpyrrolidin-3-amine; (R)-4-(3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 7-isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,7a,8,9,10-hexahydropyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazepin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-8-isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (R)-1-((S)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; (R)-1-((R)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; 4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[(3R)-3-aminopyrrolidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[3-(methylamino)azetidin-1-yl]-6a,7,8,9,9a,10-hexahydro-6H-cyclopenta[e]pyrimido[5,4-b][1,4]oxazepin-2-amine; 4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclobutane-1,7'-pyrimido[5,4-b][1,4]oxazin]-2'-amine; 7,7-dimethyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 8-ethyl-4-[3-(methylamino)azetidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 4-[(3R)-3-aminopyrrolidin-1-yl]-8-ethyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 8-ethyl-4-[(4aR,7aR)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (3R)-1-(8-ethyl-8-methyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-4-yl)-N-methylpyrrolidin-3-amine; 8-ethyl-8-methyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclopentane-1,7'-pyrimido[5,4-b][1,4]oxazin]-2'-amine; (3R)-N-methyl-1-(6′H,8′H-spiro[cyclopentane-1,7′-pyrimido[5,4-b][1,4]oxazin]-4′-yl)pyrrolidin-3-amine; (3R)-1-(3,3-difluoro-6′H,8′H-spiro[cyclobutane-1,7′-pyrimido[5,4-b][1,4]oxazin]-4′-yl)-N-methylpyrrolidin-3-amine; 7-ethyl-7-methyl-4-[(3R)-3-(methylamino)pyrrolidin-1-yl]-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 3,3-difluoro-4'-[(3R)-3-(methylamino)pyrrolidin-1-yl]-6'H,8'H-spiro[cyclobutane-1,7'-pyrimido[5,4-b][1,4]oxazin]-2'-amine; 2. The compound of claim 1, or a salt thereof, selected from the group consisting of:

20. (R)-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-ethyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-(methoxymethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-(2-methoxypropan-2-yl)-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7,7-dimethyl-4-(3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 6-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-(methylamino)pyrrolidin-1-yl)-6a,7,8,9-tetrahydro-6H-pyrimido[5,4-b]pyrrolo[1,2-d][1,4]oxazin-2-amine; (R)-4-((R)-3-aminopyrrolidin-1-yl)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; 4-((R)-3-aminopyrrolidin-1-yl)-7-(trifluoromethyl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (S)-7-((R)-1-methoxyethyl)-4-(3-(methylamino)azetidin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-7-isopropyl-4-(piperazin-1-yl)-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-2-amine; (R)-1-((R)-7-isopropyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-yl)pyrrolidin-3-amine; (R)-8-methyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-((R)-3-(methylamino)pyrrolidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-4-((R)-3-aminopyrrolidin-1-yl)-8-isopropyl-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; (S)-8-Isopropyl-4-(3-(methylamino)azetidin-1-yl)-6,7,8,9-tetrahydropyrimido[5,4-b][1,4]oxazepin-2-amine; 20. The compound of claim 19, selected from the group consisting of:

21. 21. A compound according to any one of claims 1 to 20 having H4 receptor activity.

22. 22. The compound of claim 21, which exhibits low hERG activity.

23. 23. A pharmaceutical composition comprising a compound according to any one of claims 1 to 22 and a pharmaceutically acceptable excipient.

24. 24. A compound or composition according to any one of claims 1 to 23 for use in medicine.

25. 24. A compound or composition according to any one of claims 1 to 23 for use in the treatment of inflammatory disorders including asthma, chronic pruritus, dermatitis, rheumatoid arthritis, gastric ulcerogenesis and colitis.

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