Processes and intermediates for preparing JAK1 inhibitors

Abstract

The present invention relates to processes for preparing itacitinib, or salts thereof, and related synthetic intermediates associated therewith.

Description

[Technical Field]

[0001] The present invention relates to processes for preparing itacitinib, its salts, and related synthetic intermediate compounds and salts thereof. Itacitinib and its salts are useful as inhibitors of the Janus kinase family of protein tyrosine kinases (JAKs) for the treatment of inflammatory diseases, myeloproliferative disorders, and other diseases. [Background technology]

[0002] Protein kinases (PKs) are a group of enzymes that regulate diverse and crucial biological processes, including cell proliferation, survival, differentiation, organogenesis, morphogenesis, angiogenesis, tissue repair, and regeneration, among others. Protein kinases exert their physiological functions by catalyzing the phosphorylation of proteins (or substrates), thereby regulating the cellular activity of the substrates in various biological contexts. In addition to their function in normal tissues and organs, many protein kinases also play specialized roles in a host of human diseases, including cancer. A subset of protein kinases (also known as oncogenic protein kinases), when dysregulated, can trigger tumor formation and growth and further contribute to tumor maintenance and progression (Blume-Jensen P. et al., Nature 2001, 411(6835):355-365). To date, oncogenic protein kinases represent one of the largest and most attractive groups of proteins targeted for cancer intervention and drug development.

[0003] Protein kinases can be classified into receptor and non-receptor types. Receptor tyrosine kinases (RTKs) have an extracellular portion, a transmembrane domain, and an intracellular portion, while non-receptor tyrosine kinases are entirely intracellular. The Janus kinase family of protein tyrosine kinases (JAKs) belongs to the non-receptor type of tyrosine kinases and includes the following family members: JAK1 (also known as Janus kinase-1), JAK2 (also known as Janus kinase-2), JAK3 (also known as Janus kinase, leukocyte, JAKL, L-JAK, and Janus kinase-3), and TYK2 (also known as protein tyrosine kinase-2).

[0004] Pathways involving JAKs and signal transducers and activators of transcription (STATs) are involved in the signal transduction of a wide range of cytokines. Cytokines are small polypeptides or glycoproteins that stimulate biological responses in virtually all cell types. Generally, cytokine receptors lack intrinsic tyrosine kinase activity and therefore require receptor-associated kinases to propagate phosphorylation cascades. JAKs perform this function. Cytokines bind to their receptors, causing receptor dimerization, which allows JAKs to phosphorylate each other as well as specific tyrosine motifs within the cytokine receptor. STATs that recognize these phosphotyrosine motifs are recruited to the receptor and are themselves activated by JAK-dependent tyrosine phosphorylation events. Upon activation, STATs dissociate from the receptor, dimerize, translocate to the nucleus, bind to specific DNA sites, and alter transcription (Scott, MJ, CJ Godshall, et al. (2002)). "JAKs, STATs, Cytokines, and Sepsis." Clin Diagn Lab Immunol 9(6):1153-9).

[0005] The JAK family plays a role in cytokine-dependent regulation of the proliferation and function of cells involved in immune responses. The JAK / STAT pathway, particularly all four JAK family members, is thought to play a role in the pathogenesis of asthma, chronic obstructive pulmonary disease, bronchitis, and other related lower respiratory tract inflammatory diseases. Furthermore, multiple cytokines signaling through JAK kinases have been implicated in upper respiratory tract inflammatory diseases or conditions, such as those affecting the nose and paranasal sinuses (e.g., rhinitis and sinusitis), whether or not they are classic allergic reactions. The JAK / STAT pathway has also been implicated in ocular inflammatory diseases / conditions, including, but not limited to, iritis, uveitis, scleritis, conjunctivitis, and chronic allergic reactions. Therefore, inhibition of JAK kinases may play a beneficial role in the treatment of these diseases.

[0006] Blocking signal transduction at the level of JAK kinases holds promise for developing therapies for human cancer. Inhibition of JAK kinases is expected to provide therapeutic benefits to patients suffering from skin immune disorders and skin sensitization, such as psoriasis. Therefore, inhibitors of Janus kinases or related kinases are widely sought, and several publications have reported effective classes of compounds. For example, the JAK inhibitor itacitinib (2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile) is reported in U.S. Patent Application Publication Nos. 2011 / 0224190 and 2015 / 0065484, the disclosures of which are incorporated herein by reference.

[0007] In view of the increasing demand for compounds for the treatment of disorders associated with the inhibition of kinases, such as Janus kinase, new and more efficient routes to itacitinib, its salts, and related intermediates are needed. The processes and compounds described herein help to meet these and other needs. Summary of the Invention

[0008] The present disclosure provides, inter alia, processes for preparing itacitinib, its salts, and related synthetic intermediate compounds and salts of the intermediates.

[0009] Accordingly, the present disclosure provides a process for preparing itacitinib, or a salt thereof, the process comprising: [ka] or a salt thereof, by combining (i) a salt of formula 2a and (ii) a compound of formula 2b: [ka] (In the formula, X - is a counter anion).

[0010] In some embodiments, the process for preparing itacitinib, or a salt thereof, provided herein comprises: Compound of Formula 50: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, and (ii) a compound of formula 2b: [ka] to form a compound of formula 51: [ka] (In the formula, X - is the counter anion, and P 50 is an amino protecting group).

[0011] The present disclosure further provides a process for preparing itacitinib, or a salt thereof, the process comprising reacting itacitinib with a compound of formula 2c: [ka] with a salt of a compound of formula 3: [ka] or a salt thereof to form itacitinib or a salt thereof.

[0012] The present disclosure further provides a process for preparing itacitinib, or a salt thereof, the process comprising reacting itacitinib with a compound of formula 2c: [ka] with a compound of formula 50: [ka] or a salt thereof to give a compound of formula 51: [ka] (P in the formula 50 is an amino protecting group) or a salt thereof, The compound of formula 51 is deprotected to give a compound of formula 52: [ka] or a salt thereof, and A compound of formula 52 or a salt thereof can be reacted with a compound of formula 53: [ka] in the presence of a coupling agent and a base to form itacitinib, or a salt thereof.

[0013] In some embodiments, the salt of Formula 2c is represented by Formula 2d: [ka] is prepared by a process comprising reacting with a base to form a salt of formula 2c.

[0014] In some embodiments, the salt of formula 2d is Formula 2P: [ka] with MeMgBr in the presence of a Grignard catalyst to form a compound of formula 1aP; [ka] The compound of formula 1aP is deprotected to give the compound of formula 1a: [ka] or a salt thereof, and reacting a compound of formula 1a or a salt thereof with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form a salt of formula 2d; In the formula, P 1 is an amino protecting group.

[0015] In some embodiments, the salt of formula 2d is Formula 22P: [ka] with MeMgBr in the presence of a Grignard catalyst to form a compound of formula 23P; [ka] The compound of formula 23P is reduced to give the compound of formula 1a: [ka] or a salt thereof, and reacting a compound of formula 1a or a salt thereof with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form a salt of formula 2d; In the formula, P 2 is an amino protecting group.

[0016] The present disclosure further provides a method for determining the function of Formula 50: [ka] or a salt thereof, wherein P 50 is an amino protecting group.

[0017] The present disclosure further provides a compound of Formula 3: [ka] or a salt thereof.

[0018] The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims. [Brief explanation of the drawings]

[0019] [Figure 1] 1 is an X-ray powder diffraction (XRPD) pattern of Compound 2d, Form I. [Figure 2] 1 is a differential scanning calorimetry (DSC) thermogram of Compound 2d, Form I. [Figure 3] 1 is a thermogravimetric analysis (TGA) thermogram of Compound 2d, Form I. [Figure 4] 1 is an XRPD pattern of Compound 2d, Form II. [Figure 5] 1 is a DSC thermogram of Compound 2d, Form II. [Figure 6] 1 is a TGA thermogram of Compound 2d, Form II. [Figure 7] 1 is an XRPD pattern of compound 2 hexafluorophosphate. [Figure 8] 1 is a DSC thermogram of Compound 2 hexafluorophosphate. [Figure 9] 1 is a TGA thermogram of Compound 2 hexafluorophosphate. DETAILED DESCRIPTION OF THE INVENTION

[0020] Processes and Intermediates The present disclosure provides processes for preparing the selective JAK1 inhibitor itacitinib, also known as 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile, and intermediates thereof, as well as salts and crystalline forms of itacitinib and intermediates. Itacitinib (also known as INCB039110) has the following structure: [ka] 2-(3-(4-(7H-Pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile is also referred to in the present disclosure as Compound 1. This compound and various processes for preparing the compound are disclosed in U.S. Patent Application Publication Nos. 2011 / 0224190, 2013 / 0060026, 2014 / 0256941, and 2015 / 0065484, which are incorporated herein by reference. Itacitinib is a potent JAK1 inhibitor that is more than 10-fold more selective than JAK2 and JAK3.

[0021] The present disclosure provides a process for preparing itacitinib, or a salt thereof, comprising: Compounds of Formula 3: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, or a salt thereof, and (ii) a compound of formula 2b: [ka] (X in the formula - is a counter anion).

[0022] The present disclosure provides a process for preparing itacitinib, or a salt thereof, the process comprising: Compounds of Formula 3: [ka] or a salt thereof with a reagent selected from (i) a salt of Formula 2a, and (ii) a compound of Formula 2b: [ka] (In the formula, X - is a counter anion).

[0023] In some embodiments, the reagent is a salt of Formula 2a.

[0024] In some embodiments, the reagent is a compound of formula 2b.

[0025] In some embodiments, the reagent has formula 2a, where X - is Cl - It is the salt of the salt of

[0026] In some embodiments, the reagent has formula 2a, where X - is Cl - (which is the hydrochloride salt of

[0027] In some embodiments, about 1 to about 1.5 molar equivalents of the compound of Formula 3 or its salt relative to the reagent are utilized. In some embodiments, about 1.2 to about 1.4 molar equivalents of the compound of Formula 3 or its salt relative to the reagent are utilized. In some embodiments, about 1.3 molar equivalents of the compound of Formula 3 or its salt relative to the reagent are utilized.

[0028] In some embodiments, the reaction of the reagent with the compound of Formula 3 or a salt thereof is carried out in solvent component S1. In some embodiments, solvent component S1 comprises a polar protic solvent or a polar aprotic solvent.

[0029] In some embodiments, the solvent component S1 comprises an alcohol. In some embodiments, the solvent component S1 comprises a compound of formula C 1~6 In some embodiments, the solvent component S1 comprises ethanol. In some embodiments, the solvent component S1 is ethanol.

[0030] In some embodiments, the reaction of the compound of Formula 3 or a salt thereof with the reagent is carried out at a temperature of about 20° C. to about 30° C. In some embodiments, the reaction of the compound of Formula 3 or a salt thereof with the reagent is carried out at ambient temperature.

[0031] In some embodiments, the present application further provides a process for preparing itacitinib, or a salt thereof, the process comprising: Compound of Formula 50: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, and (ii) a compound of formula 2b: [ka] to form a compound of formula 51: [ka] (In the formula, X - is the counter anion, and P 50 is an amino protecting group).

[0032] In some embodiments, P 50 is R 50 -OC(O)-, wherein R 50 is C 1~6 It is alkyl.

[0033] In one embodiment, R 50 is methyl, ethyl, propyl, isopropyl, or butyl or t-butyl.

[0034] In some embodiments, P 50is t-butyl-OC(O)-.

[0035] In some embodiments, about 1 to about 1.5 molar equivalents of the compound of Formula 50 or its salt relative to the reagent are utilized. In some embodiments, about 1.2 to about 1.4 molar equivalents of the compound of Formula 50 or its salt relative to the reagent are utilized. In some embodiments, about 1.3 molar equivalents of the compound of Formula 50 or its salt relative to the reagent are utilized.

[0036] In some embodiments, the reaction of the reagent with the compound of Formula 50 or a salt thereof is carried out in solvent component S50. In some embodiments, solvent component S50 comprises a polar protic solvent or a polar aprotic solvent.

[0037] In some embodiments, solvent component S50 comprises an alcohol. In some embodiments, solvent component S50 comprises a compound of formula C 1~6 In some embodiments, the solvent component S50 comprises ethanol.

[0038] In some embodiments, the reaction of the compound of Formula 50 or a salt thereof with the reagent is carried out at a temperature of about 20° C. to about 30° C. In some embodiments, the reaction of the compound of Formula 50 or a salt thereof with the reagent is carried out at ambient temperature.

[0039] In some embodiments, the process for preparing itacitinib, or a salt thereof, comprises deprotecting a compound of formula 51 to obtain a compound of formula 52: [ka] or a salt thereof.

[0040] In some embodiments, deprotecting the compound of Formula 51 comprises treating the compound of Formula 51 with a strong acid A51.

[0041] In some embodiments, the strong acid A51 is HCl. In some embodiments, about 5-10 molar equivalents of HCl are used relative to the compound of Formula 51. In some embodiments, about 6-8 molar equivalents of HCl are used relative to the compound of Formula 51.

[0042] In some embodiments, the treatment of the compound of Formula 51 with strong acid A51 is carried out in solvent component S51. In some embodiments, solvent component S51 comprises a polar protic solvent and an organic solvent.

[0043] In some embodiments, the solvent component S51 comprises water, an alcohol, and a halogenated hydrocarbon. In some embodiments, the alcohol of the solvent component S50 is of formula C 1~6 Contains alkyl-OH.

[0044] In some embodiments, solvent component S51 comprises water, isopropanol, and dichloromethane.

[0045] In some embodiments, the treatment of the compound of Formula 51 with a strong acid A51 is carried out at reflux temperature.

[0046] In some embodiments, the treatment of the compound of Formula 51 with the strong acid A51 is carried out at a temperature of about 30°C to about 60°C.

[0047] In some embodiments, the treatment of the compound of Formula 51 with strong acid A51 is carried out at a temperature of about 35°C to about 50°C.

[0048] In some embodiments, the treatment of the compound of Formula 51 with the strong acid A51 is carried out at a temperature of about 40°C to about 45°C.

[0049] In some embodiments, the compound of Formula 52 or salt thereof is the dihydrochloride salt of the compound of Formula 52.

[0050] In some embodiments, the process for preparing itacitinib, or a salt thereof, comprises reacting a compound of formula 52, or a salt thereof, with a compound of formula 53: [ka] in the presence of a coupling agent to form itacitinib.

[0051] In some embodiments, the coupling agent is Sodium triacetoxyborohydride is.

[0052] In some embodiments, the reaction of a compound of Formula 52 or a salt thereof with a compound of Formula 53 is carried out in the presence of a base B53.

[0053] In some embodiments, base B53 is a tertiary amine. In some embodiments, base B53 is a tri(C 1~6 In some embodiments, base B53 is triethylamine.

[0054] In some embodiments, about 1 to about 1.5 molar equivalents of a compound of formula 53 relative to a compound of formula 52 or a salt thereof are utilized.

[0055] In some embodiments, about 1 to about 1.1 molar equivalents of a compound of formula 53 relative to a compound of formula 52 or a salt thereof are utilized.

[0056] In some embodiments, about 1 to about 3 molar equivalents of coupling agent are utilized relative to the compound of Formula 52 or a salt thereof. In some embodiments, about 2 molar equivalents of coupling agent are utilized relative to the compound of Formula 52 or a salt thereof. In some embodiments, about 1 to about 3 molar equivalents of base B53 are utilized relative to the compound of Formula 52 or a salt thereof. In some embodiments, about 2 molar equivalents of base B53 are utilized relative to the compound of Formula 52 or a salt thereof.

[0057] In some embodiments, the reaction of a compound of Formula 52 or a salt thereof with a compound of Formula 53 is carried out in solvent component S52. In some embodiments, solvent component S52 comprises an organic solvent.

[0058] In some embodiments, solvent component S52 comprises dichloromethane.

[0059] In some embodiments, the reaction of a compound of Formula 52, or a salt thereof, with a compound of Formula 53 is carried out at a temperature of about 20° C. to about 30° C. In some embodiments, the reaction of a compound of Formula 52, or a salt thereof, with a compound of Formula 53 is carried out at ambient temperature.

[0060] In some embodiments, the process for preparing itacitinib, or a salt thereof, further comprises reacting itacitinib with at least one equivalent of adipic acid to form itacitinib adipate (i.e., itacitinib adipic acid salt).

[0061] In some embodiments, the reagent is a salt of Formula 2a: X - is Cl - , Br - , I - , BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - In some embodiments, X - is Cl - , BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - In some embodiments, X - is BF4 - In some embodiments, X - is PF6 - In some embodiments, X - is AsF6 - In some embodiments, X - is SbF6 - In some embodiments, X - is ClO4 - In some embodiments, X- is Cl - is.

[0062] In some embodiments, the reagent is a compound of Formula 2b. The compound of Formula 2b can be prepared by a process comprising reacting a salt of Formula 2a with a base B1. In some embodiments, the reaction of the salt of Formula 2a with the base B1 is carried out in a solvent component S2 comprising water. In some embodiments, the base B1 is a strong base. In some embodiments, the basic material is a hydroxide. In some embodiments, the base B1 is an alkali metal hydroxide. In some embodiments, the base B1 is sodium hydride. In some embodiments, about 10 to about 15 molar equivalents of the base B1 relative to the salt of Formula 2a or its salt are utilized. In some embodiments, about 12 molar equivalents of the base B1 relative to the salt of Formula 2a or its salt are utilized. In some embodiments, the reaction of the salt of Formula 2a with the base B1 is carried out at a temperature of about -10°C to about 60°C. In some embodiments, the temperature is about 0°C to room temperature. In some embodiments, the temperature is about 40°C to about 60°C. In some embodiments, the temperature is between 0°C and room temperature, and then heated to between about 40°C and about 60°C.

[0063] In some embodiments, the salt of Formula 2a or the compound of Formula 2b is Compounds of Formula 1a: [ka] or its salts can be prepared by a process which includes reacting it with Vilsmeier reagent formed from dimethylformamide.

[0064] In some embodiments, the salt of Formula 2a or the compound of Formula 2b is Compounds of formula 5a: [ka] or its salts can be prepared by a process which includes reacting it with Vilsmeier reagent formed from dimethylformamide.

[0065] In some embodiments, the compound of Formula 5a is a salt, for example, the compound of Formula 5a is a sodium salt.

[0066] In some embodiments, the reaction of a compound of formula 5a or a salt thereof with a Vilsmeier reagent produces a compound of formula 2c: [ka]

[0067] In some embodiments, after reaction with the Vilsmeier reagent, the compound of formula 2c can be converted to a compound of formula M + X - (In the formula, M + is a counter cation). In some embodiments, the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent. In some embodiments, the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride. In some embodiments, the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, and triphosgene. In some embodiments, the chlorinating agent is oxalyl chloride. In some embodiments, the chlorinating agent is phosphorus oxychloride. In some embodiments, the chlorinating agent is triphosgene.

[0068] In some embodiments, about 1 to about 5 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 1 to about 4 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 1 to about 3 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 1 molar equivalent of chlorinating agent is utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 2 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 3 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 4 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 5 molar equivalents of chlorinating agent are utilized relative to the compound of Formula 1a or 5a, or a salt thereof.

[0069] In some embodiments, about 10 to about 25 molar equivalents of dimethylformamide are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 10 to about 20 molar equivalents of dimethylformamide are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 10 to about 15 molar equivalents of dimethylformamide are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 11 to about 14 molar equivalents of dimethylformamide are utilized relative to the compound of Formula 1a or 5a, or a salt thereof. In some embodiments, about 11 to about 13 molar equivalents of dimethylformamide are utilized relative to the compound of Formula 1a or 5a, or a salt thereof.

[0070] In some embodiments, the preparation of the Vilsmeier reagent is carried out in solvent component S3. In some embodiments, solvent component S3 comprises an organic solvent. In some embodiments, solvent component S3 comprises a polar aprotic solvent. In some embodiments, solvent component S3 comprises acetonitrile, dimethylformamide, or a combination thereof.

[0071] In some embodiments, the Vilsmeier reagent is prepared at a temperature of about -10°C to about 60°C. In some embodiments, the Vilsmeier reagent is prepared at a temperature of about -10°C to about 30°C. For example, the Vilsmeier reagent is prepared at a temperature between about -10°C and about room temperature. For example, the temperature is about 0°C to about room temperature. In some embodiments, the Vilsmeier reagent is prepared at a temperature of about room temperature to about 60°C. In some embodiments, the Vilsmeier reagent is prepared at a temperature of about 30°C to about 70°C, about 40°C to about 70°C, about 30°C to about 60°C, or about 40°C to about 60°C.

[0072] In some embodiments, the reaction of a compound of Formula 1a or 5a, or a salt thereof, with a Vilsmeier reagent is carried out at a temperature of about 40°C to about 100°C. In some embodiments, the reaction of a compound of Formula 1a or 5a, or a salt thereof, with a Vilsmeier reagent is carried out at a temperature of about 70°C to about 100°C. In some embodiments, the reaction of a compound of Formula 1a or 5a, or a salt thereof, with a Vilsmeier reagent is carried out at a temperature of about 40°C to about 60°C. For example, the Vilsmeier reagent is prepared at a temperature of about 75°C to about 80°C, 80°C to 90°C, or 85°C to 90°C.

[0073] In some embodiments, the product of the reaction with the Vilsmeier reagent has formula 2c: [ka]

[0074] In some embodiments, the salt of Formula 2a is Formula 2c: [ka] salt and formula M + X - with a salt of M + is the countercation, X - is Cl - Counter anions other than

[0075] In some embodiments, M + is the alkali metal countercation. For example, M + Li + , Na + or K + In some embodiments, M + Na + In some embodiments, X - Br - , I - , BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - For example, X - is BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - In some embodiments, X - is BF4 - In some embodiments, X - is PF6 - In some embodiments, X - is AsF6 - In some embodiments, X - is SbF6 - In some embodiments, X - is ClO4 - is.

[0076] In some embodiments, the product of the reaction with the Vilsmeier reagent has formula 2c: [ka]

[0077] In some embodiments, the salt of Formula 2a is Formula 2d: [ka] The compound can be produced by a process comprising reacting a salt of the compound with a base.

[0078] In some embodiments, the compound of Formula 2b is prepared by a process comprising reacting a salt of Formula 2d with a base B2. In some embodiments, (i) reacting a salt of Formula 2d with a base B2 and (ii) reacting a salt of Formula 2a with a compound of Formula 3 are carried out in a single pot (e.g., a single reaction vessel). In some embodiments, the reaction of the salt of Formula 2d with a base B2 is carried out in a solvent component comprising water. In some embodiments, the base B2 is a strong base. In some embodiments, the base B2 is a hydroxide base. In some embodiments, the base B2 is an alkali metal hydroxide. For example, the base B2 is sodium hydroxide. In some embodiments, the reaction of the salt of Formula 2d with a base B2 is carried out at a temperature of about −10° C. to about 15° C. In some embodiments, the compound of Formula 1a or a salt thereof is a hydrochloride salt.

[0079] In some embodiments, the compound of formula 1a or salt thereof is Formula 1aP: [ka] (In the formula, P 1 can be prepared by a process comprising deprotecting a compound of formula (I) where I is an amino-protecting group.

[0080] In some embodiments, P 1 (R 1 )3Si, wherein R 1 is C 1~6In some embodiments, R 1 is methyl, ethyl, propyl, isopropyl, butyl, or t-butyl. In some embodiments, P 1 is t-butyldimethylsilyl. In some embodiments, the deprotection is carried out by reacting the compound of Formula 1aP with a base B3. In some embodiments, base B3 is a hydroxide base. For example, base B3 is ammonium hydroxide. In some embodiments, the deprotection is carried out in solvent component S4. In some embodiments, solvent component S4 comprises a polar protic solvent. In some embodiments, solvent component S4 comprises an alcohol. In some embodiments, solvent component S4 comprises a compound of Formula C 1~6 For example, the solvent component S4 contains methanol.

[0081] In some embodiments, the compound of formula 1aP is Formula 2P: [ka] with MeMgBr in the presence of a Grignard catalyst, In the formula, P 1 is an amino protecting group.

[0082] In some embodiments, the catalyst is an iron catalyst. In some embodiments, the iron catalyst is iron(III) acetylacetonate. In some embodiments, about 1 to about 2 molar equivalents of MeMgCl relative to the compound of Formula 2P are utilized. In some embodiments, about 1% to about 10% molar equivalents of catalyst relative to the compound of Formula 2P are utilized. In some embodiments, the reaction of the compound of Formula 2P with MeMgCl is carried out in solvent component S5. In some embodiments, solvent component S5 is di-C 1~6 For example, solvent component S5 comprises tetrahydrofuran. In some embodiments, the reaction of the compound of formula 2P with MeMgCl is carried out at about -It is carried out at a temperature of 10°C to about 30°C.

[0083] In some embodiments, the compound of formula 2P is Equation 12a: [ka] to form a compound of formula 2P.

[0084] In some embodiments, protection is achieved by reacting a compound of Formula 12a with an alkali metal hydride and P 1 -Y, where Y is halo. 1 -Y is (R 1 )3Si—Y, where Y is halo and R 1 is C 1~6 In some embodiments, P 1 (R 1 )Si, where R 1 is C 1~6 In one embodiment, R 1 is methyl, ethyl, propyl, isopropyl, or butyl or t-butyl. In some embodiments, P 1 is t-butyldimethylsilyl. In some embodiments, the alkali metal hydride is sodium hydride. In some embodiments, about 1 to about 2 molar equivalents of alkali metal hydride relative to the compound of Formula 12a are utilized. In some embodiments, about 1 to about 2 molar equivalents of P relative to the compound of Formula 12a are utilized. 1 In some embodiments, a compound of Formula 12a is used in combination with an alkali metal hydride and P 1 The reaction of the compound of Formula 12a with -Y is carried out at a temperature of about -10° C. to about 20° C. In some embodiments, the reaction of the compound of Formula 12a with an alkali metal hydride and P 1 The reaction with -Y is carried out in solvent component S6, which comprises an organic solvent. In some embodiments, solvent component S6 comprises di-C 1~6In some embodiments, the solvent component S6 comprises tetrahydrofuran.

[0085] In some embodiments, the compound of formula 1a or salt thereof is Formula 23P: [ka] (In the formula, P 2 can be prepared by a process comprising reducing a compound of which ##STR00012## where ##STR00013## is an amino protecting group.

[0086] In some embodiments, the reduction of the compound of formula 23P is accomplished by a process comprising reacting the compound of formula 23P with hydrogen gas in the presence of a catalyst. For example, the catalyst may be Pd on carbon. 0 In some embodiments, the amount of catalyst relative to the compound of Formula 23P is about 5% to about 15% by weight. In some embodiments, the reaction of the compound of Formula 23P with hydrogen and the catalyst is carried out at a temperature of about 40° C. to about 70° C. In some embodiments, the reaction of the compound of Formula 23P with hydrogen and the catalyst is carried out at a temperature of about 50° C. to about 60° C. In some embodiments, the reaction of the compound of Formula 23P with hydrogen and the catalyst is carried out at a temperature of about 50° C. to about 55° C. In some embodiments, the reaction of the compound of Formula 23aP with hydrogen and the catalyst is carried out in solvent component S7. In some embodiments, solvent component S7 comprises a polar protic solvent. In some embodiments, solvent component S7 comprises an alcohol. In some embodiments, solvent component S7 comprises a compound of Formula C 1~6 Contains alkyl-OH. For example, solvent component S7 contains methanol.

[0087] In some embodiments, the compound of formula 23P is Formula 22P: [ka] with MeMgBr in the presence of a Grignard catalyst, In the formula, P 2 is an amino protecting group.

[0088] In some embodiments, the catalyst is an iron catalyst. For example, the iron catalyst is iron(III) acetylacetonate. In some embodiments, about 1 to about 2 molar equivalents of MeMgCl relative to the compound of Formula 22P are utilized. In some embodiments, about 1% to about 10% molar equivalents of catalyst relative to the compound of Formula 22P are utilized. In some embodiments, the reaction of the compound of Formula 22P with MeMgCl is carried out in solvent component S8. In some embodiments, solvent component S8 is di-C 1~6 The solvent component S8 may comprise an alkyl ether or a 4- to 10-membered heterocycloalkyl ether. For example, the solvent component S8 may comprise tetrahydrofuran. In some embodiments, the reaction of the compound of Formula 2P with MeMgCl is carried out at a temperature of from about -10°C to about 30°C.

[0089] In some embodiments, the compound of formula 22P is Formula 22a: [ka] to form a compound of formula 22P.

[0090] In some embodiments, protection is achieved by reacting a compound of Formula 22a with an alkali metal hydride and P 2 -Y, where Y is halo. 2 (R 1 )Si, where R 1 is C 1~6 In one embodiment, R 1 is methyl, ethyl, propyl, isopropyl, or butyl or t-butyl. In some embodiments, P 2 is t-butyldimethylsilyl. In some embodiments, the alkali metal hydride is sodium hydride.

[0091] In some embodiments, about 1 to about 2 molar equivalents of alkali metal hydride relative to the compound of Formula 22a are utilized. In some embodiments, about 1 to about 2 molar equivalents of P relative to the compound of Formula 22a are utilized. 2 In some embodiments, a compound of Formula 22a is used in combination with an alkali metal hydride and P 2 The reaction of the compound of Formula 22a with -Y is carried out at a temperature of about -10° C. to about 20° C. In some embodiments, the reaction of the compound of Formula 22a with an alkali metal hydride and P 2 The reaction with -Y is carried out in solvent component S9, which comprises an organic solvent. In some embodiments, solvent component S9 comprises di-C 1~6 For example, the solvent component S9 includes tetrahydrofuran.

[0092] In some embodiments, the compound of formula 1a or salt thereof is Formula 18a: [ka] can be prepared by a process comprising reacting a compound of formula Ia with an acid A1 to form a compound of formula Ia.

[0093] In some embodiments, acid A1 is a strong acid. For example, strong acid A1 is hydrochloric acid. In some embodiments, the reaction of the compound of formula 18a with acid A1 is carried out in solvent component S10, which comprises a polar protic solvent. In some embodiments, solvent component S10 comprises an alcohol. In some embodiments, solvent component S10 comprises a compound of formula C 1~6 For example, solvent component S10 includes isopropyl alcohol.

[0094] In some embodiments, the compound of Formula 18a or a salt thereof is Formula 17a: [ka] can be prepared by a process comprising reacting a compound of formula 17a with formamidine acetate and triethyl orthoformate to form a compound of formula 17b.

[0095] In some embodiments, about 10 to about 15 molar equivalents of formamidine acetate are utilized relative to the compound of Formula 17a. In some embodiments, about 10, about 11, about 12, about 13, about 14, or about 15 molar equivalents of formamidine acetate are utilized relative to the compound of Formula 17a. In some embodiments, about 12 molar equivalents of formamidine acetate are utilized relative to the compound of Formula 17a. In some embodiments, about 6 to about 10 molar equivalents of triethyl orthoformate are utilized relative to the compound of Formula 17a. In some embodiments, about 6, about 7, about 8, about 9, or about 10 molar equivalents of triethyl orthoformate are utilized relative to the compound of Formula 17a. For example, about 8 molar equivalents of triethyl orthoformate are utilized relative to the compound of Formula 17a. In some embodiments, the reaction of the compound of Formula 17a with formamidine acetate and triethyl orthoformate is carried out at a temperature of about 100°C to about 150°C. For example, the temperature can be about 110° C. to about 120° C. In some embodiments, the reaction of the compound of Formula 17a with formamidine acetate and triethyl orthoformate is carried out in solvent component S11, where solvent component S11 comprises a polar protic solvent. In some embodiments, solvent component S11 comprises an alcohol. In some embodiments, solvent component S11 comprises a compound of Formula C 1~6 For example, solvent component S11 includes 1-butanol.

[0096] In some embodiments, the compound of Formula 17a or a salt thereof is Formula 20a: [ka] with a compound of formula 21a: [ka] with a compound of formula 17a to form a compound of formula 17b.

[0097] In some embodiments, about 0.4 to about 1 molar equivalent of the compound of Formula 21a is utilized relative to the compound of Formula 20a. In some embodiments, the reaction of the compound of Formula 20a with the compound of Formula 21a is carried out at room temperature. In some embodiments, the reaction of the compound of Formula 20a with the compound of Formula 21a is carried out in solvent component S12, which comprises a polar aprotic solvent. For example, solvent component S12 comprises dimethylformamide.

[0098] In some embodiments, the compound of Formula 20a or salt thereof is Formula 19a: [ka] can be prepared by a process comprising reacting a compound of formula 20a with bromo-1,1-dimethoxyethane and a base B4 to form a compound of formula 20a.

[0099] In some embodiments, base B4 is an alkali metal carbonate. For example, base B4 is cesium carbonate. In some embodiments, about 1 to about 2 molar equivalents of base B4 are utilized relative to the compound of Formula 19a. In some embodiments, about 1 to about 2 molar equivalents of bromo-1,1-dimethoxyethane are utilized relative to the compound of Formula 19a. In some embodiments, the reaction of the compound of Formula 19a with bromo-1,1-dimethoxyethane is carried out at a temperature of about 70° C. to about 100° C. In some embodiments, the reaction of the compound of Formula 19a with bromo-1,1-dimethoxyethane is carried out in solvent component S13, which comprises a polar aprotic solvent. In some embodiments, solvent component S13 comprises dimethylformamide.

[0100] In some embodiments, the compound of Formula 17a or a salt thereof is Equation 16a: [ka] can be prepared by a process comprising reacting a compound of formula 17a with ethyl acetate and a base B5 to form a compound of formula 17a.

[0101] In some embodiments, base B5 is an alkali metal alkoxide. For example, in some embodiments, base B5 is potassium tert-butoxide, about 1 to about 3 molar equivalents of base B5 are utilized relative to the compound of Formula 16a. In some embodiments, about 1 to about 2 molar equivalents of ethyl acetate are utilized relative to the compound of Formula 16a. In some embodiments, about 2 molar equivalents of base B5 are utilized relative to the compound of Formula 16a. In some embodiments, the reaction of the compound of Formula 17a with ethyl acetate and base B5 is carried out at room temperature. In some embodiments, the reaction of the compound of Formula 17a with ethyl acetate and base B5 is carried out in solvent component S14, which comprises an organic solvent. In some embodiments, solvent component S14 is selected from the group consisting of di-C 1~6 For example, the solvent component S14 includes tetrahydrofuran.

[0102] In some embodiments, the compound of formula 5a or a salt thereof is Formula 27a: [ka] can be prepared by a process comprising hydrolyzing a compound of formula (I) in water in the presence of a base B6.

[0103] In some embodiments, base B6 is an alkali metal hydroxide. For example, base B6 is sodium hydroxide. In some embodiments, about 1 to about 2 molar equivalents of base B6 are utilized relative to the compound of Formula 27a. In some embodiments, about 1.5 molar equivalents of base B6 are utilized relative to the compound of Formula 27a. In some embodiments, the hydrolysis of the compound of Formula 27a is carried out at room temperature. In some embodiments, the hydrolysis of the compound of Formula 27a is carried out in solvent component S15, where solvent component S15 comprises an organic solvent. For example, solvent component S15 comprises tetrahydrofuran, acetone, or a combination thereof.

[0104] In some embodiments, the compound of formula 5a or salt thereof is a sodium salt of the compound of formula 5a. In some embodiments, the compound of formula 5a or salt thereof is a compound of formula 5a.

[0105] In some embodiments, the compound of Formula 5a can be prepared by a process comprising reacting the sodium salt of the compound of Formula 5a with a strong acid A2. For example, the strong acid A2 is hydrochloric acid. In some embodiments, (a) the reaction of the sodium salt of the compound of Formula 5a with the strong acid A2 and (b) the hydrolysis of the sodium salt of the compound of Formula 27a are carried out in a single pot.

[0106] In some embodiments, the compound of Formula 27a is Formula 26P: [ka] (P in the formula 1 is an amino protecting group) with a strong acid A3.

[0107] In some embodiments, P 1is p-toluenesulfonyl. For example, A3 is hydrochloric acid. In some embodiments, the reaction of the compound of Formula 26P with the strong acid A3 is carried out at room temperature. In some embodiments, the reaction of the compound of Formula 26P with the strong acid A3 is carried out in solvent component S16. In some embodiments, solvent component S16 is a compound of Formula C 1~6 In some embodiments, the solvent component S16 comprises ethanol.

[0108] In some embodiments, the compound of formula 26P is Formula 25P: [ka] (In the formula, P 1 is an amino protecting group) with an alkali metal alkoxide B8 to form a compound of formula 26P.

[0109] In some embodiments, about 0.1 molar equivalents of alkali metal alkoxide B8 relative to the compound of Formula 25P are utilized. In some embodiments, the reaction of the compound of Formula 25P with alkali metal alkoxide B8 is carried out at room temperature. In some embodiments, the reaction of the compound of Formula 25P with alkali metal alkoxide B8 is carried out in solvent component S17, which comprises a polar protic solvent. For example, alkali metal alkoxide B8 is sodium ethoxide. In some embodiments, solvent component S17 comprises an alcohol. In some embodiments, solvent component S17 comprises a compound of Formula C 1~6 Contains alkyl-OH. For example, solvent component S17 contains methanol.

[0110] In some embodiments, the compound of Formula 27a is Formula 25P: [ka] can be prepared by a process comprising reacting a compound of formula B9 with an alkali metal alkoxide B9 to form a compound of formula 27a.

[0111] In some embodiments, about 1 to about 2 molar equivalents of alkali metal alkoxide B9 relative to the compound of Formula 25P are utilized. In some embodiments, about 1 molar equivalent of alkali metal alkoxide B9 relative to the compound of Formula 25P is utilized. In some embodiments, the reaction of the compound of Formula 25P with alkali metal alkoxide B9 is carried out at a temperature of about 50° C. to about 80° C. In some embodiments, the reaction of the compound of Formula 25P with alkali metal alkoxide B9 is carried out in solvent component S18, which is a solvent having a formula C 1~6 Contains alkyl-OH. For example, solvent component S18 contains ethanol.

[0112] In some embodiments, the compound of formula 25P is Formula 2P: [ka] (In the formula, P 1 is an amino protecting group) with diethyl malonate and base B10.

[0113] In some embodiments, base B10 is an alkali metal carbonate. For example, base B10 is cesium carbonate. In some embodiments, the reaction of the compound of Formula 2P with base B10 is carried out at a temperature of about 40° C. to about 70° C. In some embodiments, the reaction of the compound of Formula 2P with base B10 is carried out in solvent component S19, which comprises a polar aprotic solvent. For example, solvent component S19 comprises dimethylformamide.

[0114] In some embodiments, a compound of formula 2P can be prepared by a process comprising protecting a compound of formula 12a to form a compound of formula 2P. In some embodiments, the protection comprises protecting a compound of formula 12a with a base B11 and P 1 -Y, where Y is halo. For example, P 1is p-toluenesulfonyl. In some embodiments, base B11 is an alkali metal hydroxide. For example, base B11 is sodium hydroxide. In some embodiments, the protection is achieved by reacting the compound of Formula 12a with base B11 in solvent component S20, which comprises a polar aprotic solvent. For example, solvent component S20 comprises acetone.

[0115] In some embodiments, the compound of formula 12a is Equation 11a: [ka] or a salt thereof with a strong acid A4.

[0116] In some embodiments, strong acid A4 is a strong acid. In some embodiments, the reaction of the compound of Formula 11a or a salt thereof with strong acid A4 is carried out in solvent component S21, which comprises a polar aprotic solvent. In some embodiments, solvent component S21 is a di-C 1~6 The solvent component S21 may comprise an alkyl ether or a 4- to 10-membered heterocycloalkyl ether. For example, the solvent component S21 may comprise tetrahydrofuran. In some embodiments, the reaction of the compound of Formula 11a or a salt thereof with the strong acid A4 is carried out at the reflux temperature of tetrahydrofuran.

[0117] In some embodiments, the compound of formula 11a is Equation 10a: [ka] can be prepared by a process comprising reacting a compound of formula (II) with (methoxymethyl)triphenylphosphonium chloride and a base B12.

[0118] In some embodiments, base B12 is an alkali metal alkoxide. For example, base B12 is potassium tert-butoxide. In some embodiments, the reaction of a compound of Formula 11a or a salt thereof with (methoxymethyl)triphenylphosphonium chloride and base B12 is carried out at a temperature of about 10° C. to about 30° C. In some embodiments, the reaction of a compound of Formula 11a or a salt thereof with (methoxymethyl)triphenylphosphonium chloride and base B12 is carried out in solvent component S22, which comprises a polar aprotic solvent. In some embodiments, solvent component S22 is a di-C 1~6 For example, the solvent component S22 includes tetrahydrofuran.

[0119] In some embodiments, the compound of formula 10a is Formula 9a: [ka] can be prepared by a process comprising reacting a compound of formula (I) with ammonia.

[0120] In some embodiments, the reaction of the compound of Formula 9a with ammonia is carried out at a temperature of about 40° C. to about 70° C. In some embodiments, the reaction of the compound of Formula 9a with ammonia is carried out in solvent component S23, which comprises an organic solvent. For example, solvent component S23 comprises toluene.

[0121] In some embodiments, the compound of formula 9a is Formula 8a: [ka] can be prepared by a process comprising reacting the compound of formula (I) with Vilsmeier reagent formed from dimethylformamide.

[0122] In some embodiments, the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent. In some embodiments, the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride. For example, the chlorinating agent is phosphorus oxychloride. In some embodiments, about 4 to about 6 molar equivalents (e.g., 5 molar equivalents) of the chlorinating agent are utilized relative to the compound of Formula 8a. In some embodiments, about 1 to about 3 molar equivalents (e.g., 2 molar equivalents) of dimethylformamide are utilized relative to the compound of Formula 8a. In some embodiments, the reaction of dimethylformamide with the chlorinating agent is performed at a temperature of about -10°C to about 20°C (e.g., about 0°C to about 10°C). In some embodiments, the reaction of the compound of Formula 8a with the Vilsmeier reagent is carried out at a temperature of about 80°C to about 130°C (e.g., about 90°C to about 120°C, or about 95°C to about 115°C).

[0123] In some embodiments, the compound of formula 12a is Equation 15a: [ka] can be prepared by a process comprising reacting a compound of formula (I) with a chlorinating agent.

[0124] In some embodiments, the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride. In some embodiments, the chlorinating agent is phosphorus oxychloride. In some embodiments, the reaction of the compound of Formula 15a with the chlorinating agent is carried out at a temperature of about 50° C. to about 100° C. In some embodiments, the reaction of the compound of Formula 15a with ammonia is carried out in solvent component S24, where solvent component S24 comprises an organic solvent. For example, solvent component S24 comprises toluene.

[0125] In some embodiments, the compound of formula 15a is (i) Formula 14a: [ka] with formamidine acetate and an alkali metal hydroxide to produce a compound of formula 14aa; [ka] (ii) reacting a compound of formula 14aa with a strong acid A4.

[0126] In some embodiments, the alkali metal hydride is sodium ethoxide. In some embodiments, the reaction of the compound of Formula 14a with formamidine acetate and an alkali metal hydroxide is carried out at a temperature of about 50° C. to about 100° C. In some embodiments, the reaction of the compound of Formula 14a with formamidine acetate and an alkali metal hydroxide is carried out in solvent component S25, which comprises a polar protic solvent. In some embodiments, solvent component S25 comprises an alcohol. In some embodiments, solvent component S25 comprises a compound of Formula C 1~6 For example, solvent component S25 includes methanol. For example, strong acid A4 is hydrochloric acid.

[0127] In some embodiments, the compound of formula 14a is Formula 13a: [ka] can be prepared by a process comprising reacting the compound of formula (I) with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide.

[0128] In some embodiments, the reaction of the compound of Formula 13a with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide is carried out at a temperature of about 80° C. to about 100° C. In some embodiments, the reaction of the compound of Formula 13a with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide is carried out in solvent component S26, which comprises a polar aprotic solvent. In some embodiments, solvent component S26 comprises dimethyl sulfoxide.

[0129] In some embodiments, the compound of Formula 3 or salt thereof is Formula A1: [ka] It is formed by a process comprising reacting a compound of formula (II) with hydrazine.

[0130] In some embodiments, the hydrazine is hydrazine hydrate.

[0131] In some embodiments, about 1 to about 3 molar equivalents of hydrazine relative to the compound of Formula A1 are utilized. In some embodiments, about 1.5 to about 2.5 molar equivalents of hydrazine relative to the compound of Formula A1 are utilized. In some embodiments, about 2 to about 2.2 molar equivalents of hydrazine relative to the compound of Formula A1 are utilized. In some embodiments, about 2.1 molar equivalents of hydrazine relative to the compound of Formula A1 are utilized.

[0132] In some embodiments, the reaction of the compound of Formula A1 is carried out in solvent component S27. In some embodiments, solvent component S27 comprises an organic solvent. In some embodiments, solvent component S27 comprises an aprotic organic solvent.

[0133] In some embodiments, solvent component S27 comprises acetonitrile.

[0134] In some embodiments, the reaction of the compound of Formula A1 with hydrazine is carried out at a temperature of about 20° C. to about 30° C. In some embodiments, the reaction of the compound of Formula A1 with hydrazine is carried out at ambient temperature.

[0135] In some embodiments, the compound of formula 50 or a salt thereof is Formula 54: [ka] with hydrazine to form a compound of formula 50 or a salt thereof.

[0136] In some embodiments, the hydrazine is hydrazine hydrate.

[0137] In some embodiments, about 1 to about 3 molar equivalents of hydrazine are utilized relative to the compound of Formula 54. In some embodiments, about 1.5 to about 2.5 molar equivalents of hydrazine are utilized relative to the compound of Formula 54. In some embodiments, about 2 to about 2.2 molar equivalents of hydrazine are utilized relative to the compound of Formula 54. In some embodiments, about 2.1 molar equivalents of hydrazine are utilized relative to the compound of Formula 54.

[0138] In some embodiments, the reaction of the compound of Formula 54 is carried out in solvent component S54. In some embodiments, solvent component S54 comprises an organic solvent. In some embodiments, solvent component S54 comprises an aprotic organic solvent.

[0139] In some embodiments, solvent component S54 comprises acetonitrile.

[0140] In some embodiments, the reaction of the compound of Formula 54 with hydrazine is carried out at a temperature of about 20° C. to about 30° C. In some embodiments, the reaction of the compound of Formula 54 with hydrazine is carried out at ambient temperature.

[0141] Also provided herein are compounds of formula 50: [ka] or a salt thereof, wherein P 50 is an amino protecting group.

[0142] In some embodiments, the compound of formula 50 is [ka] or a salt thereof.

[0143] Also provided herein are compounds of formula 3: [ka] or a salt thereof is provided.

[0144] In some embodiments, the compound of formula 1a or salt thereof is Equation 12a: [ka] with t-butyldimethylsilyl chloride to produce a compound of formula 12b: [ka] Reacting a compound of formula 12b with MeMgBr in the presence of a Grignard catalyst to produce a compound of formula 12c: [ka] deprotecting a compound of formula 12c to produce a compound of formula 1a or a salt thereof.

[0145] In some embodiments, the compound of formula 1a or salt thereof is Formula 22a: [ka] with t-butyldimethylsilyl chloride and MeMgBr in the presence of a Grignard catalyst to produce a compound of formula 23a; [ka] The compound of formula 23a can be prepared by a process comprising reacting the compound of formula 23a with hydrogen and palladium on carbon to produce a compound of formula 1a or a salt thereof.

[0146] In some embodiments, the process for preparing itacitinib, or a salt thereof, comprises reacting it with a compound of formula 2c: [ka] with a salt of formula 3: [ka] or a salt thereof to form itacitinib or a salt thereof.

[0147] In some embodiments, the process for preparing itacitinib, or a salt thereof, comprises reacting it with a compound of formula 2c: [ka] The salt of formula 50: [ka] or a salt thereof to form a compound of formula 51: [ka] (In the formula, P 50 is an amino protecting group), or a salt thereof; The compound of formula 51 is deprotected to give the compound of formula 52: [ka] or a salt thereof, and The compound of formula 52, or a salt thereof, is reacted with a compound of formula 53: [ka] in the presence of a coupling agent and a base to form itacitinib, or a salt thereof.

[0148] In some embodiments, the salt of Formula 2c is represented by Formula 2d: [ka] is prepared by a process comprising reacting with a base to form a salt of formula 2c.

[0149] In some embodiments, the salt of formula 2d is Formula 2P: [ka] with MeMgBr in the presence of a Grignard catalyst to form a compound of formula 1aP; [ka] The compound of formula 1aP is deprotected to give the compound of formula 1a: [ka] or a salt thereof, and reacting a compound of formula 1a or a salt thereof with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form a salt of formula 2d; In the formula, P 1 is an amino protecting group.

[0150] In some embodiments, the salt of formula 2d is Formula 22P: [ka] with MeMgBr in the presence of a Grignard catalyst to form a compound of formula 23P; [ka] The compound of formula 23P is reduced to give the compound of formula 1a: [ka] or a salt thereof, and reacting a compound of formula 1a or a salt thereof with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form a salt of formula 2d; In the formula, P 2 is an amino protecting group.

[0151] The present application further provides itacitinib, or a salt thereof, prepared according to the processes provided herein.

[0152] The present application further provides salts of itacitinib prepared according to the processes provided herein.

[0153] The present application further provides Itacitinib prepared according to the processes provided herein.

[0154] The present application further provides itacitinib or a pharmaceutically acceptable salt thereof prepared according to the processes provided herein.

[0155] The present application further provides pharmaceutically acceptable salts of itacitinib prepared according to the processes provided herein.

[0156] The present application further provides itacitinib adipic acid salt prepared according to the processes provided herein.

[0157] At various places in the present specification, substituents of compounds of the invention are disclosed in groups or in ranges. It is specifically intended that the invention include each and every individual subcombination of the members of such groups and ranges. For example, "C 1~6The term "alkyl" is specifically intended to individually disclose methyl, ethyl, C3 alkyl, C4 alkyl, C5 alkyl, and C6 alkyl.

[0158] It will be further understood that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination.

[0159] In some embodiments, reagent or solvent components may be referenced by numbers (e.g., solvent component S1 or base B1). These numbers may be omitted in some embodiments, as they exist solely to further the antecedents of subsequent dependent claims.

[0160] For compounds of the invention in which a variable appears more than once, each variable can be a different moiety independently selected from the group defining the variable. For example, when a structure is described having two R groups simultaneously present on the same compound, the two R groups can represent different moieties independently selected from the group defined for R. As another example, optionally multiple substituents can be: [ka] When shown in the form, it is understood that the substituent R can occur p times on the ring and R can be a different moiety at each occurrence. Each R group can be a (CH) n It is understood that any hydrogen atom bonded to a ring atom can be replaced, including one or both of the hydrogen atoms. Additionally, in the above example, when the variable Q is defined to include a hydrogen, such as when Q is CH, NH, etc., any floating substituent, such as R in the above example, can replace a hydrogen of the Q variable, as well as a hydrogen of the other non-variable components of the ring.

[0161] As used herein, the term "alkyl," used alone or in combination with other terms, refers to a saturated hydrocarbon group that may be straight-chained or branched. In some embodiments, the alkyl group contains 1 to 12, 1 to 8, or 1 to 6 carbon atoms. Examples of alkyl moieties include, but are not limited to, chemical groups such as methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl, isobutyl, and sec-butyl; and higher homologs such as 2-methyl-1-butyl, n-pentyl, 3-pentyl, n-hexyl, 1,2,2-trimethylpropyl, n-heptyl, and n-octyl. In some embodiments, the alkyl moiety is methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, or 2,4,4-trimethylpentyl. In some embodiments, the alkyl moiety is methyl.

[0162] As used herein, the terms "halo" and "halogen," employed alone or in combination with other terms, refer to fluoro, chloro, bromo, and iodo.

[0163] As used herein, the term "4- to 10-membered heterocycloalkyl ether" refers to a non-aromatic ring or ring system having at least one oxygen heteroatom ring member and 4 to 10 ring members, optionally containing one or more alkenylene groups as part of the ring structure. The term "heterocycloalkyl" includes monocyclic 4-, 5-, 6-, and 7-membered heterocycloalkyl groups. Examples of 4- to 10-membered heterocycloalkyl ethers include tetrahydrofuran, tetrahydropyran, dioxane, and the like.

[0164] The processes described herein can be monitored according to any suitable method known in the art. For example, product formation can be monitored by spectroscopic means, such as nuclear magnetic resonance spectroscopy (e.g., 1 H or 13C), infrared spectroscopy, or spectrophotometry (e.g., UV-visible light), or by chromatography, such as high performance liquid chromatography (HPLC) or thin layer chromatography (TLC), or other related techniques.

[0165] As used herein, the terms "reacting" and "contacting" are used as known in the art and generally refer to bringing together chemical reagents in a manner that allows their interaction at the molecular level to achieve a chemical or physical change. In some embodiments, the reaction involves two reagents, where one or more equivalents of the second reagent are used relative to the first reagent. The reaction steps of the processes described herein can be carried out for times and under conditions suitable for the preparation of the specified product.

[0166] The compounds of the present invention also include pharmaceutically acceptable salts of the compounds described herein. As used herein, the term "pharmaceutically acceptable salts" refers to salts formed by adding a pharmaceutically acceptable acid or base to a compound disclosed herein. As used herein, the term "pharmaceutically acceptable" refers to a substance that is toxicologically acceptable for use in pharmaceutical applications and does not adversely interact with the active ingredient. Pharmaceutically acceptable salts, including mono- and di-salts, include, but are not limited to, those derived from organic and inorganic acids, such as, but not limited to, acetic acid, lactic acid, citric acid, cinnamic acid, tartaric acid, succinic acid, fumaric acid, maleic acid, malonic acid, mandelic acid, malic acid, oxalic acid, propionic acid, hydrochloric acid, hydrobromic acid, phosphoric acid, nitric acid, sulfuric acid, glycolic acid, pyruvic acid, methanesulfonic acid, ethanesulfonic acid, toluenesulfonic acid, salicylic acid, benzoic acid, and similar known acceptable acids. Lists of suitable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418 and Journal of Pharmaceutical Science, 66, 2 (1977), each of which is incorporated herein by reference in its entirety.

[0167] Preparation of compounds can involve the protection and deprotection of various chemical groups. The need for protection and deprotection and the selection of appropriate protecting groups can be easily determined by one skilled in the art. The chemical properties of protecting groups can be found, for example, in Greene, et al., Protective Groups in Organic Synthesis, 4th Ed., Wiley & Sons, 2007 (incorporated herein by reference in its entirety). Adjustments to the protecting groups and methods of formation and cleavage described herein can be made as necessary to take into account various substituents.

[0168] The reactions of the processes described herein can be carried out in a suitable solvent that can be easily selected by one skilled in the art of organic synthesis. A suitable solvent can be substantially non-reactive with the starting materials (reactants), intermediates, or products at the temperature at which the reaction is carried out, for example, a temperature that can range from the freezing temperature of the solvent to the boiling temperature of the solvent. A given reaction can be carried out in one solvent or a mixture of two or more solvents. Depending on the specific reaction step, a suitable solvent for a particular reaction step can be selected. In some embodiments, the reaction can be carried out in the absence of a solvent, such as when at least one of the reagents is a liquid or gas.

[0169] Suitable solvents may include halogenated solvents such as carbon tetrachloride, bromodichloromethane, dibromochloromethane, bromoform, chloroform, bromochloromethane, dibromomethane, butyl chloride, dichloromethane, ethylene tetrachloride, trichloroethylene, 1,1,1-trichloroethane, 1,1,2-trichloroethane, 1,1-dichloroethane, 2-chloropropane, α,α,α-trifluorotoluene, 1,2-dichloroethane, 1,2-dibromoethane, hexafluorobenzene, 1,2,4-trichlorobenzene, 1,2-dichlorobenzene, chlorobenzene, fluorobenzene, mixtures thereof, and the like.

[0170] Suitable solvents may include ether solvents such as dimethoxymethane, tetrahydrofuran, 1,3-dioxane, 1,4-dioxane, furan, diethyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, triethylene glycol dimethyl ether, anisole, t-butyl methyl ether, mixtures thereof, and the like.

[0171] Suitable protic solvents may include, by way of example and without limitation, water, methanol, ethanol, 2-nitroethanol, 2-fluoroethanol, 2,2,2-trifluoroethanol, ethylene glycol, 1-propanol, 2-propanol, 2-methoxyethanol, 1-butanol, 2-butanol, I-butyl alcohol, t-butyl alcohol, 2-ethoxyethanol, diethylene glycol, 1-pentanol, 2-pentanol, or 3-pentanol, neo-pentyl alcohol, t-pentyl alcohol, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, cyclohexanol, benzyl alcohol, phenol, or glycerol, mixtures thereof, and the like.

[0172] Suitable aprotic solvents may include, by way of example and without limitation, tetrahydrofuran (THF), N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMA), 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU), 1,3-dimethyl-2-imidazolidinone (DMI), N-methylpyrrolidinone (NMP), formamide, N-methylacetamide, N-methylformamide, acetonitrile, dimethyl sulfoxide, propionitrile, ethyl formate, methyl acetate, hexachloroacetone, acetone, ethyl methyl ketone, ethyl acetate, sulfolane, N,N-dimethylpropionamide, tetramethylurea, nitromethane, nitrobenzene or hexamethylphosphoramide, mixtures thereof, and the like.

[0173] Suitable hydrocarbon solvents may include benzene, cyclohexane, pentane, hexane, toluene, cycloheptane, methylcyclohexane, heptane (e.g., n-heptane), ethylbenzene, m-xylene, o-xylene, or p-xylene, octane, indane, nonane, or naphthalene, mixtures thereof, and the like.

[0174] Supercritical carbon dioxide and ionic liquids can also be used as solvents.

[0175] The reactions of the processes described herein can be carried out at any suitable temperature, which can be readily determined by one skilled in the art. The reaction temperature will depend, for example, on the melting and boiling points of the reagents and solvent (if present), the thermodynamics of the reaction (e.g., a highly exothermic reaction may need to be carried out at a lower temperature), and the kinetics of the reaction (e.g., a high activation energy barrier may require a higher temperature). "High temperature" refers to a temperature greater than room temperature (about 22°C).

[0176] The reactions of the processes described herein can be carried out in air or under an inert atmosphere. Typically, reactions involving reagents or products that are substantially reactive with air can be carried out using air-sensitive synthetic techniques well known to those skilled in the art.

[0177] In some embodiments, preparation of compounds may involve the addition of acids or bases, for example to effect catalysis of a desired reaction or the formation of salt forms (such as acid addition salts).

[0178] Exemplary acids may be inorganic or organic. Inorganic acids include hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, and nitric acid. Organic acids include formic acid, acetic acid, propionic acid, butanoic acid, benzoic acid, 4-nitrobenzoic acid, methanesulfonic acid, p-toluenesulfonic acid, benzenesulfonic acid, tartaric acid, trifluoroacetic acid, propiolic acid, butyric acid, 2-butynoic acid, vinylacetic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, and decanoic acid.

[0179] Exemplary bases include alkali metal hydroxides (e.g., lithium hydroxide, sodium hydroxide, and potassium hydroxide) and alkali metal carbonates (e.g., lithium carbonate, sodium carbonate, potassium carbonate, and sodium bicarbonate). Some exemplary strong bases include, but are not limited to, hydroxides, alkoxides, metal amides, metal hydrides, metal dialkylamides, and arylamines, where alkoxides include the lithium, sodium, and potassium salts of methyl oxide, ethyl oxide, and t-butyl oxide, metal amides include sodium amide, potassium amide, and lithium amide, metal hydrides include sodium hydride, potassium hydride, and lithium hydride, and metal dialkylamides include the sodium and potassium salts of methyl-, ethyl-, n-propyl-, i-propyl-, n-butyl-, t-butyl-, trimethylsilyl-, and cyclohexyl-substituted amides.

[0180] The present invention also includes salt forms of the compounds described herein.Examples of salts (salt forms) include, but are not limited to, inorganic or organic acid salts of basic residues such as amines, alkali or organic salts of acidic residues such as carboxylic acids, etc. Generally, salt forms can be prepared by reacting a free base or acid with a stoichiometric amount or excess of the desired salt-forming inorganic or organic acid or base in a suitable solvent or various combinations of solvents.A list of suitable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418, the disclosure of which is incorporated herein by reference in its entirety.

[0181] In carrying out the preparation of compounds according to the processes described herein, conventional isolation and purification procedures such as concentration, filtration, extraction, solid phase extraction, recrystallization, chromatography, and the like can be used to isolate the desired product.

[0182] In some embodiments, the compounds of the present invention and salts thereof are substantially isolated. "Substantially isolated" means that the compound is at least partially or substantially separated from the environment in which it was formed or detected. Partial separation can include, for example, a composition enriched for the compound of the present invention. Substantial separation can include a composition containing at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, or at least about 99% by weight of the compound of the present invention or a salt thereof. Methods for isolating compounds and their salts are routine in the art.

[0183] In some embodiments, itacitinib, intermediates for preparing itacitinib reagents, and salts thereof may include both anhydrous forms of the substance and solvated / hydrated forms of the substance. Different forms of the same substance have different bulk properties, for example, with respect to hygroscopicity, solubility, stability, etc. Forms with high melting points often have good thermodynamic stability, which is advantageous for extending the shelf life of drug formulations containing solid forms. Forms with low melting points are often less thermodynamically stable, but are advantageous in that they result in increased water solubility and increased drug bioavailability. Forms with low hygroscopicity are desirable for their stability to heat and humidity and resistance to degradation during long-term storage.

[0184] In some embodiments, solid forms of Compound 1, intermediates for preparing Compound 1, and salts thereof are crystalline. In some embodiments, salts of Compound 1 provided herein (e.g., phosphate salts of Compound 1) are crystalline. As used herein, the terms "crystalline" or "crystalline form" refer to a particular lattice configuration of a crystalline substance. Different crystalline forms of the same substance generally have different crystal lattices (e.g., unit cells), which result from different physical properties unique to each crystalline form. In some cases, the different lattice configurations have different water or solvent contents.

[0185] The different solid forms and salt forms thereof can be identified by solid state characterization methods such as X-ray powder diffraction (XRPD). Other characterization methods, such as differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic vapor sorption (DVS), solid state NMR, etc., can further aid in the identification of the form as well as the determination of stability and solvent / water content.

[0186] An XRPD pattern of reflections (peaks) is typically considered a fingerprint of a particular crystalline form. It is well known that the relative intensities of XRPD peaks can vary widely, particularly depending on sample preparation techniques, crystal size distribution, various filters used, sample mounting procedures, and the specific instrument employed. In some cases, new peaks may be observed or existing peaks may disappear, depending on the type or settings of the instrument. As used herein, the term "peak" refers to a reflection having a relative height / intensity of at least about 4% of the maximum peak height / intensity. Furthermore, instrumental variations and other factors can affect 2θ values. Thus, peak assignments such as those reported herein may vary by plus or minus about 0.2° (2θ), and the terms "substantially" and "about," as used herein in the context of XRPD, are meant to encompass such variations.

[0187] Similarly, temperature readings for DSC, TGA, or other thermal experiments can vary by about ±3° C. depending on the instrument, the particular settings, sample preparation, etc. Thus, crystalline forms reported herein having DSC thermograms "substantially" as shown in any of the figures or the term "about" are understood to accommodate such variations.

[0188] Generally, the term "about" means ±10%. In some embodiments, the term "about" means ±5%.

[0189] In some embodiments, the solid and salt forms are substantially isolated. "Substantially isolated" means that the solid, salt, or crystalline form is at least partially or substantially separated from the environment in which it was formed or detected. Partial separation can include, for example, compositions enriched in the solid and salt forms. Substantial separation can include compositions containing at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, or at least about 99% by weight of the solid and salt forms. Methods for isolating the solid and salt forms are routine in the art.

[0190] In some embodiments, the solid forms and salt forms described herein may be found together with other substances, such as water and solvents (e.g., hydrates and solvates), or may be isolated.

[0191] The phrase "pharmaceutically acceptable" is used herein to refer to salts, materials, compositions, and / or dosage forms that are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without undue toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit / risk ratio.

[0192] The salt-forming reactions described herein can be carried out at any suitable temperature, which can be readily determined by one skilled in the art. The reaction temperature will depend, for example, on the melting and boiling points of the reagents and solvent (if present), the thermodynamics of the reaction (e.g., highly exothermic reactions may need to be carried out at lower temperatures), and the kinetics of the reaction (e.g., high activation energy barriers may require higher temperatures).

[0193] As used herein, the expressions "ambient temperature" and "room temperature" or "rt" are art-recognized and generally refer to a temperature near the temperature of the room in which the reaction is carried out, e.g., from about 20°C to about 30°C, e.g., the reaction temperature.

[0194] Protecting groups as described herein (e.g., P 1 or P 2 ) include, but are not limited to, the amine protecting groups described in Wuts and Greene, Protective Groups in Organic Synthesis, 4th ed., John Wiley & Sons: New Jersey, pages 696-887 (and, inparticular, pages 872-887) (2007), which is incorporated herein by reference in its entirety. Examples of protecting groups described herein include CH2OC(=O)C(CH3)3, CHOCH2CH2Si(CH3)3, benzyloxycarbonyl (Cbz), 2,2,2-trichloroethoxycarbonyl (Troc), 2-(trimethylsilyl)ethoxycarbonyl (Teoc), 2-(4-trifluoromethylphenylsulfonyl)ethoxycarbonyl (Tsc), t-butoxycarbonyl (BOC), 1-adamantyloxycarbonyl (Adoc), 2-adamantylcarbonyl (2-Adoc), 2,4-dimethylaminomethyl ... Tylpent-3-yloxycarbonyl (Doc), cyclohexyloxycarbonyl (Hoc), 1,1-dimethyl-2,2,2-trichloroethoxycarbonyl (TcBOC), vinyl, 2-chloroethyl, 2-phenylsulfonylethyl, allyl, benzyl, 2-nitrobenzyl, 4-nitrobenzyl, diphenyl-4-pyridylmethyl, N',N'-dimethylhydrazinyl, methoxymethyl, t-butoxymethyl (Bum), benzyloxymethyl (BOM), 2-tetrahydropyranyl (THP), tri(C 1~4 In some embodiments, the protecting group is a tri(C 1~4In some embodiments, the protecting group is t-butyldimethylsilyl. In some embodiments, the protecting group is p-toluenesulfonyl.

[0195] In some embodiments, one or more constituent atoms of the compounds (products or synthetic intermediates) presented herein can be replaced or substituted with an isotope of the atom at natural or non-natural abundance. In some embodiments, the compounds contain at least one deuterium atom. For example, in some embodiments, one or more hydrogen atoms in the compounds presented herein can be replaced or substituted with deuterium (e.g., -CD3 replaces -CH3). 1~6 (One or more hydrogen atoms of the alkyl group may be replaced with deuterium atoms.) In some embodiments, the compound contains two or more deuterium atoms. In some embodiments, the compound contains 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, or 1-8 deuterium atoms.

[0196] In some embodiments, one or more hydrogen atoms of itacitinib, or a salt thereof, are replaced by deuterium atoms.

[0197] In some embodiments, three or more hydrogen atoms of itacitinib, or a salt thereof, are replaced by deuterium atoms.

[0198] In some embodiments, 50 or more hydrogen atoms of itacitinib, or a salt thereof, are replaced by deuterium atoms.

[0199] In some embodiments, 51 or more hydrogen atoms of itacitinib, or a salt thereof, are replaced by deuterium atoms.

[0200] Embodiment 1. A process for preparing itacitinib, or a salt thereof, comprising: Compounds of Formula 3: [ka] or a salt thereof, by combining (i) a salt of formula 2a and (ii) a compound of formula 2b: [ka] (In the formula, X - is a counter anion).

[0201] 2. The process of embodiment 1, wherein about 1 to about 1.5 molar equivalents of the compound of formula 3 or salt thereof relative to the reagent is utilized.

[0202] 3. The process of embodiment 1, wherein about 1.2 to about 1.4 molar equivalents of the compound of formula 3 or salt thereof relative to the reagent is utilized.

[0203] 4. The process of embodiment 1, wherein about 1.3 molar equivalents of the compound of formula 3 or a salt thereof relative to the base reagent is utilized.

[0204] 5. The process of any one of embodiments 1 to 4, wherein the reaction of the reagent with the compound of formula 3 or salt thereof is carried out in solvent component S1.

[0205] 6. The process of embodiment 5, wherein the solvent component S1 comprises a polar protic solvent or a polar aprotic solvent.

[0206] 7. The process of embodiment 5 or 6, wherein the solvent component S1 comprises an alcohol.

[0207] 8. The solvent component S1 is a compound of formula C 1~6 The process of any one of embodiments 5 to 7, comprising alkyl-OH.

[0208] 9. The process of any one of embodiments 5-8, wherein the solvent component S1 comprises ethanol.

[0209] 10. The process of any one of embodiments 5 to 9, wherein the reacting of the compound of formula 3 or salt thereof with a reagent is carried out at a temperature of about 20°C to about 30°C.

[0210] 11. The process of any one of embodiments 5 to 9, wherein the reacting of the compound of formula 3 or salt thereof with a reagent is carried out at ambient temperature.

[0211] 12. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 50: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, and (ii) a compound of formula 2b: [ka] to form a compound of formula 51: [ka] (In the formula, X - is the counter anion, and P 50 is an amino protecting group.

[0212] 13.P 50 R 50 -OC(O)-, wherein R 50 is C 1~6 13. The process of embodiment 12, wherein the alkyl is alkyl.

[0213] 14.In the formula, R 50 14. The process of embodiment 13, wherein is methyl, ethyl, propyl, isopropyl, butyl, or t-butyl.

[0214] 15.In the formula, P 50 13. The process of embodiment 12, wherein is t-butyl-OC(O)—.

[0215] 16. The process of any one of embodiments 12-15, wherein about 1 to about 1.5 molar equivalents of the compound of formula 50 or salt thereof relative to the reagent are utilized.

[0216] 17. The process of any one of embodiments 12-15, wherein about 1.2 to about 1.4 molar equivalents of the compound of formula 50 or salt thereof relative to the reagent are utilized.

[0217] 18. The process of any one of embodiments 12-15, wherein about 1.3 molar equivalents of the compound of formula 50 or salt thereof relative to the reagent is utilized.

[0218] 19. The process of any one of embodiments 12-18, wherein the reacting of the reagent with the compound of formula 50 or salt thereof is carried out in solvent component S50.

[0219] 20. The process of embodiment 19, wherein the solvent component S50 comprises a polar protic solvent or a polar aprotic solvent.

[0220] 21. The process of embodiment 19 or 20, wherein the solvent component S50 comprises an alcohol.

[0221] 22. The solvent component S50 is a compound represented by the formula C 1~6 22. The process of any one of embodiments 19 to 21, comprising alkyl-OH.

[0222] 23. The process of any one of embodiments 19-22, wherein the solvent component S50 comprises ethanol.

[0223] 24. The process of any one of embodiments 12-23, wherein the reacting of the compound of formula 50 or salt thereof with a reagent is carried out at a temperature of about 20°C to about 30°C.

[0224] 25. The process of any one of embodiments 12-23, wherein the reacting of the compound of formula 50 or salt thereof with a reagent is carried out at ambient temperature.

[0225] 26. Deprotecting the compound of formula 51 to give a compound of formula 52: [ka] 26. The process of any one of embodiments 12 to 25, further comprising forming HCl, HCl, or a salt thereof.

[0226] 27. The process of embodiment 26, wherein said deprotecting said compound of formula 51 comprises treating said compound of formula 51 with a strong acid A51.

[0227] 28. The process of embodiment 27, wherein the strong acid A51 is HCl.

[0228] 29. The process of embodiment 28, wherein about 5 to 10 molar equivalents of HCl relative to the compound of formula 51 are used.

[0229] 30. The process of embodiment 28, wherein about 6 to 8 molar equivalents of HCl relative to the compound of formula 51 are used.

[0230] 31. The process of any one of embodiments 26-30, wherein the treating is carried out in solvent component S51.

[0231] 32. The process of embodiment 31, wherein the solvent component S51 comprises a polar protic solvent and an organic solvent.

[0232] 33. The process of embodiment 31 or 32, wherein the solvent component S51 comprises water, an alcohol, and a halogenated hydrocarbon.

[0233] 34. The alcohol of the solvent component S50 is a compound of formula C 1~6 34. The process of any one of embodiments 31 to 33, comprising alkyl-OH.

[0234] 35. The process of any one of embodiments 31-34, wherein the solvent component S51 comprises water, isopropanol, and dichloromethane.

[0235] 36. The process of any one of embodiments 26-35, wherein said treating said compound of Formula 51 with strong acid A51 is carried out at reflux temperature.

[0236] 37. The process of any one of embodiments 26-35, wherein said treating said compound of Formula 51 with strong acid A51 is carried out at a temperature of from about 30°C to about 60°C.

[0237] 38. The process of any one of embodiments 26-35, wherein said treating said compound of Formula 51 with strong acid A51 is carried out at a temperature of about 35°C to about 50°C.

[0238] 39. The process of any one of embodiments 26-35, wherein said treating said compound of Formula 51 with strong acid A51 is carried out at a temperature of about 40°C to about 45°C.

[0239] 40. The process of any one of embodiments 26-39, wherein the compound of formula 52 or a salt thereof is the dihydrochloride salt of the compound of formula 52.

[0240] 41. The compound of formula 52 or a salt thereof is reacted with a compound of formula 53: [ka] in the presence of a coupling agent to form itacitinib.

[0241] 42. The process of embodiment 41, wherein the coupling agent is sodium triacetoxyborohydride.

[0242] 43. The process of embodiment 41 or 42, wherein the reacting of the compound of formula 52 or a salt thereof with a compound of formula 53 is carried out in the presence of a base B53.

[0243] 44. The process of embodiment 43, wherein the base B53 is a tertiary amine.

[0244] 45. The base B53 is tri-(C 1~6 45. The process of embodiment 43 or 44, wherein the amine is an alkyl)amine.

[0245] 46. ​​The process of any one of embodiments 43-45, wherein the base B53 is triethylamine.

[0246] 47. The process of any one of embodiments 41-46, utilizing about 1 to about 1.5 molar equivalents of the compound of formula 53 relative to the compound of formula 52 or salt thereof.

[0247] 48. The process of any one of embodiments 41-46, wherein about 1 to about 1.1 molar equivalents of the compound of formula 53 relative to the compound of formula 52 or salt thereof are utilized.

[0248] 49. The process of any one of embodiments 41-48, wherein about 1 to about 3 molar equivalents of coupling agent relative to the compound of formula 52 or salt thereof is utilized.

[0249] 50. The process of any one of embodiments 41-48, wherein about 2 molar equivalents of coupling agent are utilized relative to the compound of formula 52 or salt thereof.

[0250] 51. The process of any one of embodiments 43-50, wherein about 1 to about 3 molar equivalents of base B53 relative to the compound of formula 52 or salt thereof is utilized.

[0251] 52. The process of any one of embodiments 43-50, wherein about 2 molar equivalents of the base B53 relative to the compound of formula 52 or salt thereof are utilized.

[0252] 53. The process of any one of embodiments 41-52, wherein the reacting of the compound of formula 52 or salt thereof with the compound of formula 53 is carried out in solvent component S52.

[0253] 54. The process of embodiment 53, wherein the solvent component S52 comprises an organic solvent.

[0254] 55. The process of embodiment 53 or 54, wherein the solvent component S52 comprises dichloromethane.

[0255] 56. The process of any one of embodiments 41-55, wherein the reacting of the compound of formula 52 or salt thereof with the compound of formula 53 is carried out at a temperature of from about 20°C to about 30°C.

[0256] 57. The process of any one of embodiments 41-55, wherein the reacting of the compound of formula 52 or salt thereof with the compound of formula 53 is carried out at ambient temperature.

[0257] 58. The process of any one of embodiments 1-11 and 41-57, further comprising reacting itacitinib with at least 1 equivalent of adipic acid to form itacitinib adipate.

[0258] 59. The process of any one of embodiments 1-58, wherein the reagent is a salt of formula 2a.

[0259] 60.X - But Cl - , Br - , I - , BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - 60. The process of any one of embodiments 1 to 59, wherein the process is selected from:

[0260] 61.X - But Cl - , BF4 - , PF6 - , AsF6 - , SbF6 - , and ClO4 - 60. The process of any one of embodiments 1 to 59, wherein the process is selected from:

[0261] 62.X - But BF4 - 60. The process of any one of embodiments 1 to 59, wherein

[0262] 63.X - But PF6 - 60. The process of any one of embodiments 1 to 59, wherein

[0263] 64.X - But AsF6 - 60. The process of any one of embodiments 1 to 59, wherein

[0264] 65.X - But SbF6 - 60. The process of any one of embodiments 1 to 59, wherein

[0265] 66.X - But ClO4 - 60. The process of any one of embodiments 1 to 59, wherein

[0266] 67.X - But Cl - 60. The process of any one of embodiments 1 to 59, wherein

[0267] 68. The process of any one of embodiments 1-58, wherein the reagent is a compound of formula 2b.

[0268] 69. The process of any one of embodiments 1-58 and 68, wherein the compound of formula 2b is prepared by a process comprising reacting the salt of formula 2a with a base B1.

[0269] 70. The process of embodiment 69, wherein the reaction of the salt of Formula 2a with the base B1 is carried out in a solvent component S2 comprising water.

[0270] 71. The process according to embodiment 69 or 70, wherein the base B1 is a strong base.

[0271] 72. The process of any one of embodiments 69-71, wherein the base B1 is a hydroxide.

[0272] 73. The process of any one of embodiments 69-72, wherein the base B1 is an alkali metal hydroxide.

[0273] 74. The process of any one of embodiments 69-73, wherein the base B1 is sodium hydroxide.

[0274] 75. The process of any one of embodiments 69-74, wherein about 10 to about 15 molar equivalents of the base B1 relative to the salt of Formula 2a or salt thereof are utilized.

[0275] 76. The process of any one of embodiments 69-74, wherein about 12 molar equivalents of the base B1 relative to the salt of formula 2a or salt thereof are utilized.

[0276] 77. The process of any one of embodiments 69-76, wherein the reaction of the salt of Formula 2a with the base B1 is carried out at a temperature of from about -10°C to about 60°C.

[0277] 78. The salt of formula 2a or the compound of formula 2b is The compound of formula 1a: [ka] 78. The process of any one of embodiments 1-77, wherein the compound is prepared by a process comprising reacting 2-(2-methyl-2-propanol), or a salt thereof, with Vilsmeier reagent formed from dimethylformamide.

[0278] 79. The salt of formula 2a or the compound of formula 2b is The compound of formula 5a: [ka] 78. The process of any one of embodiments 1-77, wherein the compound is prepared by a process comprising reacting 2-(2-methyl-2-propanol), or a salt thereof, with Vilsmeier reagent formed from dimethylformamide.

[0279] 80. The process of embodiment 79, wherein the compound of formula 5a or a salt thereof is a salt.

[0280] 81. The process of embodiment 79 or 80, wherein said compound of formula 5a or salt thereof is a sodium salt.

[0281] 82. The process of any one of embodiments 78-81, wherein the reaction with the Vilsmeier reagent produces a compound of formula 2c: [ka]

[0282] 83. After the reaction with Vilsmeier reagent, the compound of formula 2c can be converted to a compound of formula M + X - (In the formula, M + 83. The process of embodiment 82, wherein R is a counter cation.

[0283] 84. The process of any one of embodiments 78-83, wherein the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent.

[0284] 85. The process of embodiment 84, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride.

[0285] 86. The process of embodiment 84, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, and triphosgene.

[0286] 87. The process of embodiment 84, wherein the chlorinating agent is oxalyl chloride.

[0287] 88. The process of embodiment 84, wherein the chlorinating agent is phosphorus oxychloride.

[0288] 89. The process of embodiment 84, wherein the chlorinating agent is triphosgene.

[0289] 90. The process of any one of embodiments 84-89, wherein about 1 to about 5 molar equivalents of chlorinating agent relative to said compound of formula 1a or 5a, or salt thereof, are utilized.

[0290] 91. The process of any one of embodiments 84-89, wherein about 1 to about 4 molar equivalents of chlorinating agent relative to said compound of formula 1a or 5a, or salt thereof, are utilized.

[0291] 92. The process of any one of embodiments 84-89, wherein about 1 to about 3 molar equivalents of chlorinating agent relative to said compound of formula 1a or 5a, or salt thereof, are utilized.

[0292] 93. The process of any one of embodiments 78-92, wherein about 10 to about 25 molar equivalents of dimethylformamide relative to the compound of formula 1a or 5a, or salt thereof, are utilized.

[0293] 94. The process of any one of embodiments 78-92, wherein about 10 to about 20 molar equivalents of dimethylformamide relative to the compound of formula 1a or 5a, or salt thereof, are utilized.

[0294] 95. The process of any one of embodiments 78-92, wherein about 10 to about 15 molar equivalents of dimethylformamide relative to the compound of formula 1a or 5a, or salt thereof, are utilized.

[0295] 96. The process of any one of embodiments 78-92, wherein about 11 to about 14 molar equivalents of dimethylformamide relative to the compound of formula 1a or 5a, or salt thereof, are utilized.

[0296] 97. The process of any one of embodiments 78-92, wherein about 11 to about 13 molar equivalents of dimethylformamide relative to the compound of formula 1a or 5a, or salt thereof, are utilized.

[0297] 98. The process of any one of embodiments 78-97, wherein the preparation of the Vilsmeier reagent is carried out in solvent component S3.

[0298] 99. The process of embodiment 98, wherein the solvent component S3 comprises an organic solvent.

[0299] 100. The process of embodiment 98 or 99, wherein the solvent component S3 comprises a polar aprotic solvent.

[0300] 101. The process of any one of embodiments 98-100, wherein the solvent component S3 comprises acetonitrile, dimethylformamide, or a combination thereof.

[0301] 102. The process of any one of embodiments 78-101, wherein the Vilsmeier reagent is prepared at a temperature of about -10°C to about 60°C.

[0302] 103. The process of any one of embodiments 78-101, wherein the Vilsmeier reagent is prepared at a temperature of about -10°C to about 30°C.

[0303] 104. The process of any one of embodiments 78-101, wherein the Vilsmeier reagent is prepared at a temperature of about room temperature to about 60°C.

[0304] 105. The process of any one of embodiments 78-101, wherein the reacting of the compound of formula 1a or 5a, or a salt thereof, with the Vilsmeier reagent is carried out at a temperature of from about 40°C to about 100°C.

[0305] 106. The process of any one of embodiments 78-101, wherein the reacting of the compound of formula 1a or 5a, or a salt thereof, with the Vilsmeier reagent is carried out at a temperature of about 70°C to about 100°C.

[0306] 107. The process of any one of embodiments 78-101, wherein the reacting of the compound of formula 1a or 5a, or a salt thereof, with the Vilsmeier reagent is carried out at a temperature of about 40°C to about 60°C.

[0307] 108. The process of any one of embodiments 78-107, wherein the product of the reaction with the Vilsmeier reagent has formula 2d: [ka]

[0308] 109. The salt of formula 2a is The salt of formula 2c: [ka] , the formula M + X - with a salt of M + is the countercation, X - is Cl - 78. The process of any one of embodiments 1 to 77, wherein the counter anion is other than

[0309] 110.In the formula, M + 110. The process of embodiment 109, wherein is an alkali metal countercation.

[0310] 111.In the formula, M + Li + , Na + or K + 111. The process of embodiment 109 or 110, wherein

[0311] 112.In the formula, M + Na+ 111. The process of embodiment 109 or 110, wherein

[0312] 113.In the formula, X - But, Br - , I - , BF4 - , PF6 - , AsF6 - , SbF6 - , or ClO4 - 113. The process according to any one of embodiments 109 to 112, wherein the process is selected from:

[0313] 114. The salt of formula 2c is Formula 2d: [ka] 114. The process of any one of embodiments 109-113, wherein the compound is produced by a process comprising reacting a salt of

[0314] 115. The process according to embodiment 114, wherein the compound of formula 2b is prepared by a process comprising reacting a salt of formula 2d with a base B2.

[0315] 116. The process of embodiment 115, wherein (i) reacting the salt of formula 2d with base B2, and (ii) reacting the salt of formula 2a with the compound of formula 3, are carried out in a single pot.

[0316] 117. The process of embodiment 115 or 116, wherein the reaction of the salt of formula 2d with base B2 is carried out in a solvent component comprising water.

[0317] 118. The process according to any one of embodiments 115-117, wherein the base B2 is a strong base.

[0318] 119. The process according to any one of embodiments 115-118, wherein the base B2 is a hydroxide base.

[0319] 120. The process of any one of embodiments 115-119, wherein the base B2 is an alkali metal hydroxide.

[0320] 121. The process of any one of embodiments 115-120, wherein the base B2 is sodium hydroxide.

[0321] 122. The process of any one of embodiments 115-121, wherein the reaction of the salt of Formula 2d with base B2 is carried out at a temperature of from about -10°C to about 15°C.

[0322] 123. The process according to any one of embodiments 78 and 84-122, wherein the compound of formula 1a or salt thereof is the hydrochloride salt.

[0323] 124. The compound of formula 1a or a salt thereof is Formula 1aP: [ka] (In the formula, P 1 The process according to any one of embodiments 78 and 84 to 123, wherein the compound is prepared by a process comprising deprotecting a compound of formula (I) above,

[0324] 125.P 1 (R 1 )3Si, wherein R 1 is C 1~6 The process of embodiment 124, wherein the alkyl is alkyl.

[0325] 126.In the formula, R 1 The process of embodiment 125, wherein is methyl, ethyl, propyl, isopropyl, butyl, or t-butyl.

[0326] 127.In the formula, P 1 The process of embodiment 124, wherein is t-butyldimethylsilyl.

[0327] 128. The process according to any one of embodiments 124-127, wherein the deprotection is carried out by reacting the compound of formula 1aP with a base B3.

[0328] 129. The process of embodiment 128, wherein the base B3 is a hydroxide base.

[0329] 130. The process of embodiment 128 or 129, wherein the base B3 is ammonium hydroxide.

[0330] 131. The process of any one of embodiments 128-130, wherein the deprotection is carried out in solvent component S4.

[0331] 132. The process of embodiment 131, wherein the solvent component S4 comprises a polar protic solvent.

[0332] 133. The process of embodiment 131 or 132, wherein the solvent component S4 comprises an alcohol.

[0333] 134. The solvent component S4 is a compound represented by the formula C 1~6 The process of any one of embodiments 131 to 133, comprising alkyl-OH.

[0334] 135. The process of any one of embodiments 131-134, wherein the solvent component S4 comprises methanol.

[0335] 136. The compound of formula 1aP is Formula 2P: [ka] with MeMgBr in the presence of a Grignard catalyst, In the formula, P 1 136. The process of any one of embodiments 124-135, wherein is an amino protecting group.

[0336] 137. The process of embodiment 136, wherein the catalyst is an iron catalyst.

[0337] 138. The process of embodiment 137, wherein the iron catalyst is iron(III) acetylacetonate.

[0338] 139. The process of any one of embodiments 136-138, wherein about 1 to about 2 molar equivalents of MeMgCl relative to the compound of formula 2P are utilized.

[0339] 140. The process of any one of embodiments 136-139, wherein about 1% to about 10% molar equivalents of catalyst relative to the compound of formula 2P are utilized.

[0340] 141. The process of any one of embodiments 136-140, wherein the reaction of the compound of formula 2P with MeMgCl is carried out in solvent component S5.

[0341] 142. The solvent component S5 is di-C 1~6 The process of embodiment 141, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0342] 143. The process of embodiment 141 or 142, wherein the solvent component S5 comprises tetrahydrofuran.

[0343] 144. The process of any one of embodiments 136-143, wherein the reacting of the compound of formula 2P with MeMgCl is carried out at a temperature of from about -10°C to about 30°C.

[0344] 145. The compound of formula 2P is Equation 12a: [ka] to form said compound of formula 2P.

[0345] 146. The protection step comprises reacting the compound of formula 12a with an alkali metal hydride and P 1 146. The process of embodiment 145, comprising reacting with -Y, wherein Y is halo.

[0346] 147.P 1 -Y is (R 1 )3Si - Y, Y is halo, and R 1 C 1~6 The process of embodiment 146, wherein the alkyl is alkyl.

[0347] 148.P 1 (R 1 )Si, where R 1 is C 1~6 The process of embodiment 147, wherein the alkyl is alkyl.

[0348] 149.In the formula, R 1 149. The process of embodiment 147 or 148, wherein is methyl, ethyl, propyl, isopropyl, butyl or t-butyl.

[0349] 150.In the formula, P 1 The process of embodiment 146, wherein is t-butyldimethylsilyl.

[0350] 151. The process of any one of embodiments 146-150, wherein the alkali metal hydride is sodium hydride.

[0351] 152. The process of any one of embodiments 146-151, wherein about 1 to about 2 molar equivalents of alkali metal hydride relative to the compound of formula 12a is utilized.

[0352] 153. About 1 to about 2 molar equivalents of P relative to the compound of formula 12a 1 The process of any one of embodiments 146 to 152, wherein -Y is utilized.

[0353] 154. The compound of formula 12a with an alkali metal hydride and P1 The process of any one of embodiments 146 to 153, wherein the reaction with -Y is carried out at a temperature of from about -10°C to about 20°C.

[0354] 155. The compound of formula 12a with an alkali metal hydride and P 1 155. The process of any one of embodiments 146 to 154, wherein the reaction with -Y is carried out in solvent component S6, said solvent component S6 comprising an organic solvent.

[0355] 156. The solvent component S6 is di-C 1~6 The process of embodiment 155, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0356] 157. The process of embodiment 155 or 156, wherein the solvent component S6 comprises tetrahydrofuran.

[0357] 158. The compound of formula 1a or a salt thereof is Formula 23P: [ka] (In the formula, P 2 The process according to any one of embodiments 78 and 84 to 123, wherein the compound is prepared by a process comprising reducing a compound of formula (I) where R is an amino protecting group.

[0358] 159. The process of embodiment 158, wherein the reduction of the compound of formula 23P is achieved by a process comprising reacting the compound of formula 23P with hydrogen gas in the presence of a catalyst.

[0359] 160. The catalyst is Pd on carbon 0 160. The process of embodiment 159, wherein

[0360] 161. The process of embodiment 159 or 160, wherein the amount of the catalyst relative to the compound of formula 23P is about 5% to about 15% by weight.

[0361] 162. The process of any one of embodiments 159-161, wherein the reacting of the compound of Formula 23P with hydrogen and the catalyst is carried out at a temperature of from about 40°C to about 70°C.

[0362] 163. The process of any one of embodiments 159-162, wherein the reaction of the compound of Formula 23aP with hydrogen and the catalyst is carried out in solvent component S7.

[0363] 164. The process of embodiment 163, wherein the solvent component S7 comprises a polar protic solvent.

[0364] 165. The process of embodiment 163 or 164, wherein the solvent component S7 comprises an alcohol.

[0365] 166. The solvent component S7 is a compound represented by the formula C 1~6 166. The process of any one of embodiments 163 to 165, comprising alkyl-OH.

[0366] 167. The process of any one of embodiments 163-166, wherein the solvent component S7 comprises methanol.

[0367] 168. The compound of formula 23P is Formula 22P: [ka] with MeMgBr in the presence of a Grignard catalyst, In the formula, P 2 168. The process of any one of embodiments 158-167, wherein is an amino protecting group.

[0368] 169. The process of embodiment 168, wherein the catalyst is an iron catalyst.

[0369] 170. The process of embodiment 169, wherein the iron catalyst is iron(III) acetylacetonate.

[0370] 171. The process of any one of embodiments 168-170, wherein about 1 to about 2 molar equivalents of MeMgCl relative to the compound of formula 22P is utilized.

[0371] 172. The process of any one of embodiments 168-171, wherein about 1% to about 10% molar equivalents of the catalyst relative to the compound of formula 22P are utilized.

[0372] 173. The process of any one of embodiments 168-172, wherein the reaction of the compound of formula 22P with MeMgCl is carried out in solvent component S8.

[0373] 174. The solvent component S8 is di-C 1~6 The process of embodiment 173, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0374] 175. The process of embodiment 173 or 174, wherein the solvent component S8 comprises tetrahydrofuran.

[0375] 176. The process of any one of embodiments 168-175, wherein the reacting of the compound of formula 2P with MeMgCl is carried out at a temperature of from about -10°C to about 30°C.

[0376] 177. The compound of formula 22P is Formula 22a: [ka] to form said compound of formula 22P.

[0377] 178. The protection step comprises reacting the compound of formula 22a with an alkali metal hydride and P 2 178. The process of embodiment 177, comprising reacting with -Y, wherein Y is halo.

[0378] 179.P 2(R 1 )Si, where R 1 C 1~6 The process of embodiment 178, wherein the alkyl is alkyl.

[0379] 180.In the formula, R 1 180. The process of embodiment 179, wherein is methyl, ethyl, propyl, isopropyl, butyl or t-butyl.

[0380] 181.In the formula, P 2 The process of embodiment 178, wherein is t-butyldimethylsilyl.

[0381] 182. The process of any one of embodiments 178-181, wherein the alkali metal hydride is sodium hydride.

[0382] 183. The process of any one of embodiments 178-182, wherein about 1 to about 2 molar equivalents of said alkali metal hydride relative to said compound of Formula 22a are utilized.

[0383] 184. About 1 to about 2 molar equivalents of P relative to the compound of formula 22a 2 The process of any one of embodiments 178 to 183, wherein -Y is utilized.

[0384] 185. The compound of formula 22a with an alkali metal hydride and P 2 The process of any one of embodiments 178 to 184, wherein the reaction with -Y is carried out at a temperature of from about -10°C to about 20°C.

[0385] 186. The compound of formula 22a with an alkali metal hydride and P 2 186. The process of any one of embodiments 178 to 185, wherein the reaction with -Y is carried out in solvent component S9, said solvent component S9 comprising an organic solvent.

[0386] 187. The solvent component S9 is di-C 1~6The process of embodiment 186, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0387] 188. The process of embodiment 186 or 187, wherein the solvent component S9 comprises tetrahydrofuran.

[0388] 189. The compound of formula 1a or a salt thereof is Formula 18a: [ka] The process according to any one of embodiments 78 and 84 to 123, wherein the compound is prepared by a process comprising reacting a compound of formula Ia with an acid A1 to form said compound of formula Ia.

[0389] 190. The process of embodiment 189, wherein the acid A1 is a strong acid.

[0390] 191. The process of embodiment 189 or 190, wherein the acid A1 is hydrochloric acid.

[0391] 192. The process of any one of embodiments 189-191, wherein the reaction of the compound of Formula 18a with the acid A1 is carried out in solvent component S10, which comprises a polar protic solvent.

[0392] 193. The process of embodiment 192, wherein the solvent component S10 comprises an alcohol.

[0393] 194. The solvent component S10 is a compound represented by the formula C 1~6 194. The process of embodiment 192 or 193, comprising alkyl-OH.

[0394] 195. The process of any one of embodiments 192-194, wherein the solvent component S10 comprises isopropyl alcohol.

[0395] 196. The compound of formula 18a or a salt thereof is Formula 17a: [ka] 196. The process according to any one of embodiments 189 to 195, wherein the compound is prepared by a process comprising reacting a compound of formula 17a with formamidine acetate and triethyl orthoformate to form said compound of formula 17a.

[0396] 197. The process of embodiment 196, wherein about 10 to about 15 molar equivalents of formamidine acetate relative to said compound of formula 17a are utilized.

[0397] 198. The process of embodiment 196 or 197, wherein about 6 to about 10 molar equivalents of triethyl orthoformate relative to the compound of formula 17a are utilized.

[0398] 199. The process of any one of embodiments 196-198, wherein the reaction of the compound of Formula 17a with formamidine acetate and triethyl orthoformate is carried out at a temperature of from about 100°C to about 150°C.

[0399] 200. The process of any one of embodiments 196-199, wherein the reaction of the compound of Formula 17a with formamidine acetate and triethyl orthoformate is carried out in solvent component S11, and solvent component S11 comprises a polar protic solvent.

[0400] 201. The process of embodiment 200, wherein the solvent component S11 comprises an alcohol.

[0401] 202. The solvent component S11 is a compound represented by the formula C 1~6 202. The process of embodiment 200 or 201, comprising alkyl-OH.

[0402] 203. The process of any one of embodiments 200-202, wherein the solvent component S11 comprises 1-butanol.

[0403] 204. The compound of formula 17a or a salt thereof is Formula 20a: [ka] with a compound of formula 21a: [ka] with a compound of formula 17a to form said compound of formula 17b.

[0404] 205. The process of embodiment 204, wherein about 0.4 to about 1 molar equivalent of the compound of formula 21a is utilized relative to the compound of formula 20a.

[0405] 206. The process of embodiment 204 or 205, wherein the reacting of the compound of formula 20a with the compound of formula 21a is carried out at room temperature.

[0406] 207. The process of any one of embodiments 204-206, wherein the reaction of the compound of Formula 20a with the compound of Formula 21a is carried out in solvent component S12, and solvent component S12 comprises a polar aprotic solvent.

[0407] 208. The process of embodiment 207, wherein the solvent component S12 comprises dimethylformamide.

[0408] 209. The compound of formula 20a or a salt thereof is Formula 19a: [ka] The process according to any one of embodiments 204 to 208, wherein the compound is prepared by a process comprising reacting a compound of formula 20a with bromo-1,1-dimethoxyethane and a base B4 to form the compound of formula 20a.

[0409] 210. The process of embodiment 209, wherein the base B4 is an alkali metal carbonate.

[0410] 211. The process of embodiment 209 or 210, wherein the base B4 is cesium carbonate.

[0411] 212. The process of any one of embodiments 209-211, wherein about 1 to about 2 molar equivalents of said base B4 relative to said compound of Formula 19a are utilized.

[0412] 213. The process of any one of embodiments 209-212, wherein about 1 to about 2 molar equivalents of bromo-1,1-dimethoxyethane relative to the compound of formula 19a are utilized.

[0413] 214. The process of any one of embodiments 209-213, wherein the reaction of the compound of Formula 19a with bromo-1,1-dimethoxyethane is carried out at a temperature of from about 70°C to about 100°C.

[0414] 215. The process of any one of embodiments 209-214, wherein the reaction of the compound of Formula 19a with bromo-1,1-dimethoxyethane is carried out in solvent component S13, wherein solvent component S13 comprises a polar aprotic solvent.

[0415] 216. The process of embodiment 215, wherein the solvent component S13 comprises dimethylformamide.

[0416] 217. The compound of formula 17a or a salt thereof is Equation 16a: [ka] The process according to any one of embodiments 209 to 216, wherein the compound is prepared by a process comprising reacting a compound of formula 17a with ethyl acetate and a base B5 to form said compound of formula 17a.

[0417] 218. The process of embodiment 217, wherein the base B5 is an alkali metal alkoxide.

[0418] 219. The process according to embodiment 217 or 218, wherein the base B5 is potassium tert-butoxide.

[0419] 220. The process of any one of embodiments 217-219, wherein about 1 to about 3 molar equivalents of said base B5 relative to said compound of Formula 16a are utilized.

[0420] 221. The process of any one of embodiments 217-220, wherein about 1 to about 2 molar equivalents of ethyl acetate relative to the compound of formula 16a is utilized.

[0421] 222. The process of any one of embodiments 217-221, wherein the reaction of the compound of Formula 17a with ethyl acetate and base B5 is carried out at room temperature.

[0422] 223. The process of any one of embodiments 217-222, wherein the reaction of the compound of Formula 17a with ethyl acetate and base B5 is carried out in solvent component S14, wherein solvent component S14 comprises an organic solvent.

[0423] 224. The solvent component S14 is di-C 1~6 The process of embodiment 223, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0424] 225. The process of embodiment 223 or 224, wherein the solvent component S14 comprises tetrahydrofuran.

[0425] 226. The compound of formula 5a or a salt thereof is Formula 27a: [ka] The process according to embodiments 79-123, wherein the compound is prepared by a process comprising hydrolyzing the compound of formula (I) in water in the presence of a base B6.

[0426] 227. The process of embodiment 226, wherein the base B6 is an alkali metal hydroxide.

[0427] 228. The process of embodiment 227, wherein the base B6 is sodium hydroxide.

[0428] 229. The process of any one of embodiments 226-228, wherein about 1 to about 2 molar equivalents of said base B6 relative to said compound of Formula 27a are utilized.

[0429] 230. The process of any one of embodiments 226-229, wherein said hydrolysis of said compound of formula 27a is carried out at room temperature.

[0430] 231. The process of any one of embodiments 226-230, wherein the hydrolysis of the compound of Formula 27a is carried out in solvent component S15, and solvent component S15 comprises an organic solvent.

[0431] 232. The process of embodiment 231, wherein the solvent component S15 comprises tetrahydrofuran, acetone, or a combination thereof.

[0432] 233. The process of any one of embodiments 79-90, 98-104, 108, and 226-232, wherein the compound of formula 5a or salt thereof is a sodium salt of the compound of formula 5a.

[0433] 234. The process of any one of embodiments 79-90, 98-104, 108, and 226-233, wherein the compound of formula 5a or salt thereof is a compound of formula 5a.

[0434] 235. The process of embodiment 234, wherein the compound of formula 5a is prepared by a process comprising reacting the sodium salt of the compound of formula 5a with a strong acid A2.

[0435] 236. The process of embodiment 235, wherein the strong acid A2 is hydrochloric acid.

[0436] 237. The process of embodiment 235 or 236, wherein (a) the reaction of the sodium salt of the compound of formula 5a with a strong acid A2, and (b) the hydrolysis of the sodium salt of the compound of formula 27a, are carried out in a single pot.

[0437] 238. The compound of formula 27a is Formula 26P: [ka] (P in the formula 1 is an amino protecting group) with a strong acid A3.

[0438] 239.In the formula, P 1 The process of embodiment 238, wherein is p-toluenesulfonyl.

[0439] 240. The process according to embodiment 238 or 239, wherein A3 is hydrochloric acid.

[0440] 241. The process of any one of embodiments 238-240, wherein the reaction of the compound of formula 26P with a strong acid A3 is carried out at room temperature.

[0441] 242. The process of any one of embodiments 238-241, wherein the reaction of the compound of formula 26P with strong acid A3 is carried out in solvent component S16.

[0442] 243. The solvent component S16 is a compound represented by the formula C 1~6 243. The process of embodiment 242, comprising alkyl-OH.

[0443] 244. The process of embodiment 242 or 243, wherein the solvent component S16 comprises ethanol.

[0444] 245. The compound of formula 26P is Formula 25P: [ka] (In the formula, P 1 is an amino protecting group) with an alkali metal alkoxide B8 to form the compound of formula 26P.

[0445] 246. The process of embodiment 245, wherein about 0.1 molar equivalents of alkali metal alkoxide B8 relative to the compound of formula 25P is utilized.

[0446] 247. The process of embodiment 245 or 246, wherein the reaction of the compound of formula 25P with an alkali metal alkoxide B8 is carried out at room temperature.

[0447] 248. The process of any one of embodiments 245-247, wherein the reaction of the compound of formula 25P with alkali metal alkoxide B8 is carried out in solvent component S17, and solvent component S17 comprises a polar protic solvent.

[0448] 249. The process of any one of embodiments 245-248, wherein the alkali metal alkoxide B8 is sodium ethoxide.

[0449] 250. The process of embodiment 248 or 249, wherein the solvent component S17 comprises an alcohol.

[0450] 251. The solvent component S17 is a compound represented by the formula C 1~6 251. The process of any one of embodiments 248-250, comprising alkyl-OH.

[0451] 252. The process of any one of embodiments 248-250, wherein the solvent component S17 comprises ethanol.

[0452] 253. The compound of formula 27a is Formula 25P: [ka] The process according to any one of embodiments 226 to 237, wherein the compound is prepared by a process comprising reacting a compound of formula B9 with an alkali metal alkoxide B9 to form said compound of formula 27a.

[0453] 254. The process of embodiment 253, wherein about 1 to about 2 molar equivalents of alkali metal alkoxide B9 relative to the compound of formula 25P are utilized.

[0454] 255. The process of embodiment 253, wherein about 1 molar equivalent of alkali metal alkoxide B9 relative to the compound of formula 25P is utilized.

[0455] 256. The process of any one of embodiments 253-255, wherein the reacting of the compound of Formula 25P with an alkali metal alkoxide B9 is carried out at a temperature of from about 50°C to about 80°C.

[0456] 257. The reaction of the compound of formula 25P with alkali metal alkoxide B9 is carried out in solvent component S18, which is a compound of formula C 1~6 257. The process of any one of embodiments 253 to 256, comprising alkyl-OH.

[0457] 258. The process of embodiment 257, wherein the solvent component S18 comprises ethanol.

[0458] 259. The compound of formula 25P is Formula 2P: [ka] (In the formula, P 1 is an amino-protecting group) with diethyl malonate and a base B10.

[0459] 260. The process of embodiment 259, wherein the base B10 is an alkali metal carbonate.

[0460] 261. The process according to embodiments 259-260, wherein the base B10 is cesium carbonate.

[0461] 262. The process of any one of embodiments 259-261, wherein the reacting of the compound of formula 2P with base B10 is carried out at a temperature of from about 40°C to about 70°C.

[0462] 263. The process of any one of embodiments 259-262, wherein the reaction of the compound of formula 2P with base B10 is carried out in solvent component S19, wherein solvent component S19 comprises a polar aprotic solvent.

[0463] 264. The process of embodiment 263, wherein the solvent component S19 comprises dimethylformamide.

[0464] 265. The process according to any one of embodiments 259-264, wherein said compound of formula 2P is prepared by a process comprising protecting a compound of formula 12a to form said compound of formula 2P.

[0465] 266. The protection step comprises reacting the compound of formula 12a with a base B11 and P 1 266. The process of embodiment 265, comprising reacting with -Y, wherein Y is halo.

[0466] 267.In the formula, P 1 267. The process of embodiment 266, wherein is p-toluenesulfonyl.

[0467] 268. The process of embodiment 266 or 267, wherein the base B11 is an alkali metal hydroxide.

[0468] 269. The process of any one of embodiments 266-268, wherein the base B11 is sodium hydroxide.

[0469] 270. The process of any one of embodiments 266-268, wherein the protection is achieved by reacting the compound of Formula 12a with a base B11 in solvent component S20, wherein solvent component S20 comprises a polar aprotic solvent.

[0470] 271. The process of embodiment 270, wherein the solvent component S20 comprises acetone.

[0471] 272. The compound of formula 12a is Equation 11a: [ka] or a salt thereof with a strong acid A4.

[0472] 273. The process of embodiment 272, wherein the strong acid A4 is hydrochloric acid.

[0473] 274. The process of embodiment 272 or 273, wherein the reaction of the compound of formula 11a or a salt thereof with strong acid A4 is carried out in solvent component S21, wherein solvent component S21 comprises a polar aprotic solvent.

[0474] 275. The solvent component S21 is di-C 1~6 The process of embodiment 274, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0475] 276. The process of embodiment 274 or 275, wherein the solvent component S21 comprises tetrahydrofuran.

[0476] 277. The process of any one of embodiments 272-276, wherein the reaction of the compound of Formula 11a or a salt thereof with a strong acid A4 is carried out at the reflux temperature of tetrahydrofuran.

[0477] 278. The compound of formula 11a or a salt thereof is Equation 10a: [ka] or a salt thereof with (methoxymethyl)triphenylphosphonium chloride and a base B12.

[0478] 279. The process of embodiment 278, wherein the base B12 is an alkali metal alkoxide.

[0479] 280. The process of embodiment 278 or 279, wherein the base B12 is potassium t-butoxide.

[0480] 281. The process of any one of embodiments 278-280, wherein the reaction of the compound of Formula 11a or a salt thereof with (methoxymethyl)triphenylphosphonium chloride and base B12 is carried out at a temperature of from about 10°C to about 30°C.

[0481] 282. The process of any one of embodiments 278-281, wherein the reaction of the compound of Formula 11a or a salt thereof with (methoxymethyl)triphenylphosphonium chloride and base B12 is carried out in solvent component S22, wherein solvent component S22 comprises a polar aprotic solvent.

[0482] 283. The solvent component S22 is di-C 1~6 The process of embodiment 282, comprising an alkyl ether or a 4- to 10-membered heterocycloalkyl ether.

[0483] 284. The process of embodiment 282 or 283, wherein the solvent component S22 comprises tetrahydrofuran.

[0484] 285. The compound of formula 10a or a salt thereof is Formula 9a: [ka] 285. The process according to any one of embodiments 278 to 284, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with ammonia.

[0485] 286. The process of embodiment 285, wherein the reaction of the compound of Formula 9a with ammonia is carried out at a temperature of from about 40°C to about 70°C.

[0486] 287. The process of embodiment 285 or 286, wherein the reaction of the compound of formula 9a with ammonia is carried out in solvent component S23, which comprises an organic solvent.

[0487] 288. The process of embodiment 287, wherein the solvent component S23 comprises toluene.

[0488] 289. The compound of formula 9a is Formula 8a: [ka] 289. The process according to any one of embodiments 285 to 288, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with Vilsmeier reagent formed from dimethylformamide.

[0489] 290. The process of embodiment 289, wherein the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent.

[0490] 291. The process of embodiment 290, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride.

[0491] 292. The process of embodiment 290, wherein the chlorinating agent is phosphorus oxychloride.

[0492] 293. The compound of formula 12a is Equation 15a: [ka] 293. The process according to any one of embodiments 289 to 292, wherein the compound is prepared by a process comprising reacting a compound of formula (I) with a chlorinating agent.

[0493] 294. The process of embodiment 293, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, triphosgene, thionyl chloride, sulfuryl chloride, and phosphorus pentachloride.

[0494] 295. The process of embodiment 294, wherein the chlorinating agent is phosphorus oxychloride.

[0495] 296. The process of any one of embodiments 293-295, wherein the reaction of the compound of Formula 15a with a chlorinating agent is carried out at a temperature of from about 50°C to about 100°C.

[0496] 297. The process of any one of embodiments 293-296, wherein the reaction of the compound of Formula 15a with ammonia is carried out in solvent component S24, wherein solvent component S24 comprises an organic solvent.

[0497] 298. The process of embodiment 297, wherein the solvent component S24 comprises toluene.

[0498] 299. The compound of formula 15a is (i) Formula 14a: [ka] with formamidine acetate and an alkali metal hydroxide to produce the compound of formula 14aa; [ka] (ii) reacting said compound of formula 14aa with a strong acid A4.

[0499] 300. The process of embodiment 299, wherein the alkali metal hydroxide is sodium ethoxide.

[0500] 301. The process of any one of embodiments 299 or 300, wherein the reaction of the compound of Formula 14a with formamidine acetate and an alkali metal hydroxide is carried out at a temperature of from about 50°C to about 100°C.

[0501] 302. The process of any one of embodiments 299-301, wherein the reaction of the compound of Formula 14a with formamidine acetate and an alkali metal hydroxide is carried out in solvent component S25, wherein solvent component S25 comprises a polar protic solvent.

[0502] 303. The process of embodiment 302, wherein the solvent component S25 comprises an alcohol.

[0503] 304. The solvent component S25 is a compound represented by the formula C 1~6 The process of embodiment 302 or 303, comprising alkyl-OH.

[0504] 305. The process of any one of embodiments 302-304, wherein the solvent component S25 comprises ethanol.

[0505] 306. The process of any one of embodiments 299-305, wherein the strong acid A4 is hydrochloric acid.

[0506] 307. The compound of formula 14a is Formula 13a: [ka] The process according to any one of embodiments 299 to 306, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide.

[0507] 308. The process of embodiment 307, wherein the reaction of the compound of Formula 13a with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide is carried out at a temperature of from about 80°C to about 100°C.

[0508] 309. The process of embodiment 307 or 308, wherein the reaction of the compound of Formula 13a with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide is carried out in solvent component S26, which comprises a polar aprotic solvent.

[0509] 310. The process of embodiment 309, wherein the solvent component S26 comprises dimethyl sulfoxide.

[0510] 311. The compound of formula 3 or a salt thereof is Formula A1: [ka] 311. The process of any one of embodiments 1-310, wherein the compound is formed by a process comprising reacting a compound of formula (I) with hydrazine.

[0511] 312. The process of embodiment 311, wherein the hydrazine is hydrazine hydrate.

[0512] 313. The process of embodiment 311 or 312, wherein about 1 to about 3 molar equivalents of hydrazine relative to the compound of formula A1 are utilized.

[0513] 314. The process of embodiment 311 or 312, wherein about 1.5 to about 2.5 molar equivalents of hydrazine relative to the compound of formula A1 are utilized.

[0514] 315. The process of embodiment 311 or 312, wherein about 2 to about 2.2 molar equivalents of hydrazine relative to the compound of formula A1 are utilized.

[0515] 316. The process of embodiment 311 or 312, wherein about 2.1 molar equivalents of hydrazine relative to the compound of formula A1 are utilized.

[0516] 317. The process of any one of embodiments 311-316, wherein the reaction of the compound of formula A1 is carried out in solvent component S27.

[0517] 318. The process of embodiment 317, wherein the solvent component S27 comprises an organic solvent.

[0518] 319. The process of embodiment 317 or 318, wherein the solvent component S27 comprises an aprotic organic solvent.

[0519] 320. The process of any one of embodiments 317-319, wherein the solvent component S27 comprises acetonitrile.

[0520] 321. The process of any one of embodiments 311-320, wherein the reaction of the compound of formula A1 with hydrazine is carried out at a temperature of from about 20°C to about 30°C.

[0521] 322. The process of any one of embodiments 311-320, wherein the reaction of the compound of formula A1 with hydrazine is carried out at ambient temperature.

[0522] 323. The compound of formula 50 or a salt thereof, Formula 54: [ka] The process according to any one of embodiments 311 to 322, wherein the compound is prepared by a process comprising reacting a compound of formula 50 with hydrazine to form said compound of formula 50 or a salt thereof.

[0523] 324. The process of embodiment 323, wherein the hydrazine is hydrazine hydrate.

[0524] 325. The process of embodiment 323 or 324, wherein about 1 to about 3 molar equivalents of hydrazine relative to the compound of formula 54 is utilized.

[0525] 326. The process of embodiment 323 or 324, wherein about 1.5 to about 2.5 molar equivalents of hydrazine relative to the compound of formula 54 are utilized.

[0526] 327. The process of embodiment 323 or 324, wherein about 2 to about 2.2 molar equivalents of hydrazine relative to the compound of formula 54 are utilized.

[0527] 328. The process of embodiment 323 or 324, wherein about 2.1 molar equivalents of hydrazine relative to the compound of formula 54 are utilized.

[0528] 329. The process of any one of embodiments 323-328, wherein the reaction of the compound of Formula 54 is carried out in solvent component S54.

[0529] 330. The process of embodiment 329, wherein the solvent component S54 comprises an organic solvent.

[0530] 331. The process of embodiment 329 or 330, wherein the solvent component S54 comprises an aprotic organic solvent.

[0531] 332. The process of any one of embodiments 329-331, wherein the solvent component S54 comprises acetonitrile.

[0532] 333. The process of any one of embodiments 323-332, wherein the reaction of the compound of Formula 54 with hydrazine is carried out at a temperature of from about 20°C to about 30°C.

[0533] 334. The process of any one of embodiments 323-332, wherein the reaction of the compound of Formula 54 with hydrazine is carried out at ambient temperature.

[0534] 335.The following formula: [ka] (In the formula, P 50 is an amino protecting group), or a salt thereof.

[0535] 336.The following formula: [ka] or a salt thereof.

[0536] 337.The following formula: [ka] or a salt thereof.

[0537] The present invention will now be described in more detail by way of specific examples. The following examples are provided for illustrative purposes and are not intended to limit the invention in any manner. Those skilled in the art will readily recognize a variety of non-critical parameters that can be changed or modified to yield essentially the same results. [Example]

[0538] 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile and its adipic acid salt were prepared according to the following scheme.

[0539] [ka] [ka]

[0540] Example 1. Preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (Compound 2 chloride hydrochloride) [ka] A solution of oxalyl chloride (21.88 g, 15.1 mL, 169 mmol, 2.25 equiv.) in anhydrous acetonitrile (65 mL) was cooled to 0-5 °C in an ice bath. Anhydrous DMF (70.8 g, 75.0 mL, 969 mmol, 12.9 equiv.) was added dropwise to the solution to form the corresponding Vilsmeier reagent. The internal temperature was controlled below 10 °C during the DMF addition. The ice batch was removed, and the reaction mixture was gradually warmed to ambient temperature over 40 min. Methyl-7H-pyrrolo[2,3-d]pyrimidine (1a, 10.0 g, 75.1 mmol) was added in one portion to the in situ generated Vilsmeier reagent as a solid at ambient temperature. The resulting slurry was stirred at ambient temperature for 5-10 min to ensure mixing, then warmed to 85-90 °C. The reaction mixture was stirred at 85-90 °C for 1 h and then gradually cooled to ambient temperature. Anhydrous tetrahydrofuran (THF, 100 mL) was charged, and the resulting slurry was stirred at ambient temperature for 2 hours, followed by 2 hours at 0–5 °C. The solid was collected by filtration, washed with a 1:1 mixture of THF and MTBE (2 × 100 mL), and dried under vacuum to constant weight to give the desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (2d, 24.38 g, theoretical 23.72 g, 98.9% by HPLC area %, 90.2% by NMR weight %, 92.6% yield) as a yellow to brown crystalline solid (Form I) containing 6–7% DMF and acetonitrile and 1–2% water, which was used in the next reaction without further purification. For compound 2d: 1 HNMR (500MHz, DMSO-d6) δ13.65(s,1H), 8.99(s,1H), 8.48(s,2H), 7.99-7.94(m,1H), 6.84(dd, J=3.6, 1.6Hz, 1H), 3.48(s, 6H), 2.82(s, 6H)ppm; 13C NMR (DMSO-d6, 125MHz) δ163.8, 151.3, 147.6, 145.0, 132.1, 117.5, 102.9, 91.6, 48.9, 42.1ppm;C 13 H 19 Cl2N5 (MW, 279.77 for compound 2c, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0541] Crystalline Form I of compound 2d was characterized by XRPD, DSC and TGA.

[0542] X-ray powder diffraction (XRPD): X-ray powder diffraction (XRPD) data were obtained using a Bruker D8 Advance ECO X-ray powder diffractometer (XRPD) instrument. The general experimental procedure for XRPD was: (1) copper X-ray emission at 1.5418 Å and a LYNXEYE™ detector; (2) X-ray power 40 KV, 25 mA; and (3) sample powder was dispersed on a zero-background sample holder. The general measurement conditions for XRPD were: start angle 3 degrees; stop angle 30 degrees; sampling 0.015 degrees; and a scan rate of 2 degrees / min.

[0543] Form I of compound 2d was confirmed to be a crystalline solid by XRPD analysis. The XRPD pattern of crystalline Form I of compound 2d is shown in Figure 1, and the peak data is shown in Table 1. [Table 1]

[0544] Differential Scanning Calorimetry (DSC): DSC was performed on a TA Instruments Differential Scanning Calorimeter, Discovery DSC2500, equipped with an autosampler. DSC instrument conditions: 20-300 °C at 10 °C / min, T-zero aluminum sample pan and lid, and nitrogen gas flow at 50 mL / min. DSC analysis of compound 2d crystalline form I revealed a single endothermic peak with an onset temperature of 55.6 °C and a maximum at 100.6 °C. The DSC thermogram of compound 2d crystalline form I is provided in Figure 2.

[0545] Thermogravimetric Analysis (TGA): TGA was obtained from a TA Instruments Thermogravimetric Analyzer, Discovery TGA5500, equipped with an autosampler. General experimental conditions for TGA: 25°C to 300°C ramp at 10°C / min, nitrogen purge gas flow rate of 25 mL / min, platinum sample holder. TGA analysis of compound 2d crystalline form I showed an 8.0% weight loss below 100°C and significant weight loss due to decomposition above 175°C. The TGA thermogram of compound 2d crystalline form I is provided in Figure 3.

[0546] Example 2: Alternative preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (compound 2d) A solution of oxalyl chloride (43.76 g, 30.2 mL, 338 mmol, 2.25 equiv.) in anhydrous acetonitrile (130 mL) was cooled to 0-5 °C in an ice bath. Anhydrous DMF (141.6 g, 140.0 mL, 1938 mmol, 12.9 equiv.) was added dropwise to the solution to form the corresponding Vilsmeier reagent. The internal temperature was controlled below 10 °C during the DMF addition. The ice bath was removed, and the reaction mixture was allowed to gradually warm to ambient temperature over 40 min. Methyl-7H-pyrrolo[2,3-d]pyrimidine hydrochloride (compound 1a hydrochloride, 25.44 g, 150 mmol) was added in one portion to the in situ generated Vilsmeier reagent as a solid at ambient temperature. The resulting slurry was stirred at ambient temperature for 5-10 min to ensure mixing, then warmed to 85-90 °C. The reaction mixture was stirred at 85–90 °C for 1 h and then gradually cooled to ambient temperature. Anhydrous tetrahydrofuran (THF, 200 mL) was added, and the resulting slurry was stirred at ambient temperature for 48 h, followed by 2 h at 0–5 °C. The solid was collected by filtration, washed with a 1:1 mixture of THF and MTBE (2 × 200 mL), and dried under vacuum to constant weight to give the desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (Compound 2d), 46.17 g (47.43 g theoretical, 99.5% area by HPLC, 95.2% by weight by NMR, 92.7% yield), as a yellow to brown crystalline solid (Form II) containing 2.3% DMF and acetonitrile and 0.8% water, which was used in the next reaction without further purification. For compound 2d: 1 HNMR (500MHz, DMSO-d6) δ13.65(s,1H), 8.99(s,1H), 8.48(s,2H), 7.99-7.94(m,1H), 6.84(dd, J=3.6, 1.6Hz, 1H), 3.48(s, 6H), 2.82(s, 6H)ppm; 13 C NMR (DMSO-d6, 125MHz) δ163.8, 151.3, 147.6, 145.0, 132.1, 117.5, 102.9, 91.6, 48.9, 42.1ppm;C 13 H 19Cl2N5 (MW, 279.77 for compound 2c, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0547] Crystalline Form II of compound 2d was characterized by XRPD, DSC and TGA.

[0548] X-ray powder diffraction (XRPD): X-ray powder diffraction (XRPD) data were obtained using a Bruker D8 Advance ECO X-ray powder diffractometer (XRPD) instrument. The general experimental procedure for XRPD was: (1) copper X-ray emission at 1.5418 Å and a LYNXEYE™ detector; (2) X-ray power 40 KV, 25 mA; and (3) sample powder was dispersed on a zero-background sample holder. The general measurement conditions for XRPD were: start angle 3 degrees; stop angle 30 degrees; sampling 0.015 degrees; and a scan rate of 2 degrees / min.

[0549] Crystalline Form II of Compound 2d was confirmed to be a crystalline solid by XRPD analysis. The XRPD pattern of crystalline Form II of Compound 2d is shown in Figure 4, and the peak data are shown in Table 2. [Table 2]

[0550] Differential Scanning Calorimetry (DSC): DSC was performed on a TA Instruments Differential Scanning Calorimeter, Discovery DSC2500, equipped with an autosampler. DSC instrument conditions: 20-300 °C at 10 °C / min, T-zero aluminum sample pan and lid, and nitrogen gas flow at 50 mL / min. DSC analysis of compound 2d crystalline form II revealed a single endothermic peak with an onset temperature of 46.6 °C and a maximum at 99.2 °C. The DSC thermogram of compound 2d crystalline form II is provided in Figure 5.

[0551] Thermogravimetric Analysis (TGA): TGA was obtained from a TA Instruments Thermogravimetric Analyzer, Discovery TGA 5500, equipped with an autosampler. General experimental conditions for TGA: 25°C to 300°C ramp at 10°C / min, nitrogen purge gas flow rate of 25 mL / min, platinum sample holder. TGA analysis of compound 2d crystalline form II showed a 4.7% weight loss below 150°C and significant weight loss due to decomposition above 175°C. The TGA thermogram of compound 2d crystalline form II is provided in Figure 6.

[0552] Example 3: Alternative preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (compound 2d) [ka] A solution of phosphorus oxochloride (POCl3, 17.25 g, 10.5 mL, 112.5 mmol, 1.5 equiv.) in anhydrous acetonitrile (65 mL) was cooled to 0-5 °C in an ice bath. Anhydrous DMF (70.8 g, 70.0 mL, 968 mmol, 12.9 equiv.) was added dropwise to the solution to form the corresponding Vilsmeier reagent. The internal temperature was controlled below 10 °C during the addition of DMF. The ice batch was removed, and the reaction mixture was allowed to warm gradually to ambient temperature. Methyl-7H-pyrrolo[2,3-d]pyrimidine hydrochloride (compound 1a hydrochloride, 12.72 g, 75.0 mmol) was added in one portion to the in situ generated Vilsmeier reagent as a solid at ambient temperature. The resulting slurry was stirred at ambient temperature for 5-10 min to ensure mixing, then warmed to 75-80 °C. The reaction mixture was stirred at 75-80 °C for 1 h and then gradually cooled to ambient temperature. Anhydrous tetrahydrofuran (THF, 100 mL) was added, and the resulting slurry was stirred at ambient temperature for 2 h, followed by 2 h at 0-5 °C. The solid was collected by filtration and washed with a 1:1 mixture of THF and MTBE (2 × 100 mL) to give the desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (Compound 2d, 27.83 g, theoretical 23.72 g, 96.1% by HPLC area %, 69.0% by NMR weight %, 81.0% yield), as a yellow to brown crystalline (Form I) solid containing 11.49% DMF and acetonitrile, and 1.38% water, which was used in the next reaction without further purification. For Compound 2d: 1 HNMR (500MHz, DMSO-d6) δ13.65(s,1H), 8.99(s,1H), 8.48(s,2H), 7.99-7.94(m,1H), 6.84(dd, J=3.6, 1.6Hz, 1H), 3.48(s, 6H), 2.82(s, 6H)ppm; 13 C NMR (DMSO-d6, 125MHz) δ163.8, 151.3, 147.6, 145.0, 132.1, 117.5, 102.9, 91.6, 48.9, 42.1ppm;C 13 H 19Cl2N5 (MW, 279.77 for compound 2c, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0553] Example 4: Preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (Compound 2 chloride) using POCl [ka] A solution of phosphorus oxochloride (POCl3, 23.0 g, 14.0 mL, 150 mmol, 2.0 equiv.) in anhydrous acetonitrile (65 mL) was cooled to 0-5 °C in an ice bath. Anhydrous DMF (70.8 g, 70.0 mL, 968 mmol, 12.9 equiv.) was added dropwise to the solution to form the corresponding Vilsmeier reagent. The internal temperature was controlled below 10 °C during the addition of DMF. The ice batch was removed, and the reaction mixture was allowed to warm gradually to ambient temperature. Methyl-7H-pyrrolo[2,3-d]pyrimidine hydrochloride (compound 1a hydrochloride, 12.72 g, 75.0 mmol) was added in one portion to the in situ generated Vilsmeier reagent as a solid at ambient temperature. The resulting slurry was stirred at ambient temperature for 5-10 min to ensure mixing, then warmed to 75-80 °C. The reaction mixture was stirred at 75-80 °C for 1 h and then gradually cooled to ambient temperature. Anhydrous tetrahydrofuran (100 mL) was added, and the resulting slurry was stirred at ambient temperature for 2 h, followed by 2 h at 0-5 °C. The solid was collected by filtration and washed with a 1:1 mixture of THF and MTBE (2 × 100 mL) to give the desired product, (E)-N-(3-(dimethylamino)-2-(7H)-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (compound 2d) as a yellow to brown wet cake. The wet cake was then dissolved in water (120 mL), and the pH of the resulting solution was adjusted to 7-8 by treatment with 50% aqueous sodium hydroxide solution (NaOH, 19.06 g) at 0-5 °C. The neutralized solution was treated with charcoal (5.5 g) and stirred at ambient temperature for 12 h. The charcoal was removed by filtration through a Celite bed, and the Celite bed was washed with water (50 mL). The resulting aqueous solution contained the desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c, purity by HPLC area % >99.0%), which was used in the subsequent reaction without further treatment.

[0554] Example 5: Synthesis of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c) using triphosgene [ka] A solution of triphosgene ((CClO)CO, 37.4 g, 126 mmol, 1.5 equiv.) in anhydrous acetonitrile (73 mL) was cooled to 0–5 °C in an ice bath. Anhydrous DMF (79.0 g, 84 mL, 1083 mmol, 12.9 equiv.) was added dropwise to the solution to form the corresponding Vilsmeier reagent. The internal temperature was controlled below 10 °C during the addition of DMF. The ice batch was removed, and the reaction mixture was gradually warmed to ambient temperature over 40 min. Methyl-7H-pyrrolo[2,3-d]pyrimidine hydrochloride (compound 1a hydrochloride, 14.25 g, 84.0 mmol) was charged in one portion to the in situ generated Vilsmeier reagent as a solid at ambient temperature. The resulting slurry was stirred at ambient temperature for 5–10 min to ensure mixing, then warmed to 80–90 °C. The reaction mixture was stirred at 80-90 °C for 1 h and then gradually cooled to ambient temperature. Anhydrous tetrahydrofuran (THF, 112 mL) was added, and the resulting slurry was stirred at ambient temperature for 12 h, followed by 2 h at 0-5 °C. The solid was collected by filtration, washed with a 1:1 mixture of THF and MTBE (2 × 200 mL), and dried under vacuum to constant weight to give the desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c), 28.3 g (23.5 g theoretical, 98.8% HPLC area %, 64.9% HPLC weight %, 78.2% yield), as a yellow to brown amorphous solid containing 19.7% DMF and 0.8% water, which was used in the next reaction without further purification. For compound 2c: 1 HNMR (500MHz, DMSO-d6) δ13.65(s,1H), 8.99(s,1H), 8.48(s,2H), 7.99-7.94(m,1H), 6.84(dd, J=3.6, 1.6Hz, 1H), 3.48(s, 6H), 2.82(s, 6H)ppm; 13C NMR (DMSO-d6, 125MHz) δ163.8, 151.3, 147.6, 145.0, 132.1, 117.5, 102.9, 91.6, 48.9, 42.1ppm;C 13 H 19 Cl2N5 (MW, 279.77 for compound 2c, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0555] Example 6: Preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium salt [ka] (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium perchlorate (Compound 2 perchlorate) To a solution of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c, 2.94 g, 10.525 mmol) in water (8.06 mL) was added sodium perchlorate (NaClO, 1.933 g, 15.79 mmol, 1.50 equiv.) at ambient temperature. After stirring at 20-25 °C for 12 h, the slurry was cooled in an ice bath for 2 h. The solid was filtered, washed with cold HO (3 × 2 mL), and dried under vacuum to give the crude desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium perchlorate (compound 2 perchlorate) as a white solid, which was used in the next reaction without further purification. 1 HNMR(400MHz,DMSO-d6)δ12.50-12.17(s,1H),8.94-8.73(s,1H),8.08-7.87(s,2H),7.77-7.57(d C13 H 18 ClNO (MW, 343.77 for compound 2 perchlorate, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0556] (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium tetrafluoroborate (Compound 2 tetrafluoroborate) To a solution of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c, 2.94 g, 10.525 mmol) in water (8.06 mL) was added sodium tetrafluoroborate (NaBF, 1.733 g, 15.79 mmol, 1.50 equiv.) at ambient temperature. After stirring at 20-25 °C for 12 h, the slurry was cooled in an ice bath for 2 h. The solid was filtered, washed with cold HO (3 × 2 mL), and dried under vacuum to give the crude desired product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium tetrafluoroborate (compound 2 tetrafluoroborate, 1.80 g, theoretical 3.49 g, 51.6% yield) as a white solid, which was used in the next reaction without further purification. 1 HNMR(400MHz,DMSO-d6)δ12.39-12.34(s,1H),8.85-8.80(s,1H),7.99-7.94(s,2H),7.71-7.65(d d, J=3.4,2.2Hz, 1H), 6.52-6.46(dd, J=3.5, 1.7Hz, 1H), 3.34-3.29(s, 6H), 2.38-2.33(s, 6H)ppm; 11 BNMR(DMSO-d6, 128MHz)δ-1.27ppm; 19 F NMR (DMSO-d6, 376.5 MHz) δ -148.23 and -148.28 ppm; C 13 H 18BF4N5 (MW, 331.13 for tetrafluoroborate compound 2, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0557] (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluorophosphate (compound 2 hexafluorophosphate) Crude (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride hydrochloride (compound 2d, 25.61 g, 91.6 mmol), produced from 4-methyl-7H-pyrrolo[2,3-d]pyrimidine (12.19 g, 91.6 mmol) via the corresponding Vilsmeier reaction described in Example 1, was dissolved in water (80 mL) and an aqueous solution of sodium hydroxide (NaOH) was added at 0-5 °C to adjust the pH of the solution to 7-8. Charcoal (7.69 g) was added to the resulting aqueous solution, and the mixture was stirred at ambient temperature for 2-4 h. The charcoal was removed by filtration through a Celite bed, and the wet charcoal cake was washed with water (15 mL). Sodium hexafluorophosphate (NaPF, 20.08 g, 120 mmol, 1.31 equiv.) was then added to the combined aqueous solution at ambient temperature. After stirring at 20–25 °C for 1 h, the slurry was cooled in an ice bath for 30 min. The solid was filtered, washed with cold HO (2 × 25 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluorophosphate (compound 2 hexafluorophosphate, 24.30 g, theoretical 35.81 g, 67.9% yield, 98.7% by HPLC area%), as a white crystalline solid, which was used in the next reaction without further purification. The crude Compound 2 hexafluorophosphate can be purified by recrystallization from water to produce the pure product as a white crystalline solid. For Compound 2 hexafluorophosphate: 1HNMR (500MHz, DMSO-d6) δ12.36(s, 1H), 8.83(s, 1H), 7.97(brs, 2H), 7.68(dd, J =3.2, 2.6Hz, 1H), 6.48(dd, J=3.4, 1.8Hz, 1H), 3.32(s, 6H), 2.36(brs, 6H)ppm; 13 C NMR (125MHz, DMSO-d6) δ163.7, 152.9, 151.4, 151.0, 128.9, 120.7, 101.5, 99.8, 48.9, 40.0ppm; 19 FNMR(DMSO-d6, 470.6MHz)δ-70.2(d, 1 J(PF) = 711.1Hz) ppm; 31 PNMR (DMSO-d6, 162MHz) δ -144.19 (septet, 1 J(PF)=711Hz)ppm. C 13 H 18 F6N5P (MW, 389.29 for compound 2 hexafluorophosphate, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak). The crystallinity of compound 2 hexafluorophosphate was characterized by XRPD, DSC, and TGA.

[0558] X-ray powder diffraction (XRPD): X-ray powder diffraction (XRPD) data were obtained using a Bruker D8 Advance ECO X-ray powder diffractometer (XRPD) instrument. The general experimental procedure for XRPD was as follows: (1) copper X-ray emission at 1.5418 Å and a LYNXEYE™ detector; (2) X-ray power 40 kV, 25 mA; and (3) sample powder was dispersed on a zero-background sample holder. The general measurement conditions for XRPD were as follows: start angle 3 degrees; stop angle 30 degrees; sampling 0.015 degrees; and scan rate 2 degrees / min. XRPD analysis confirmed that compound 2 hexafluorophosphate was a crystalline solid. The XRPD pattern of compound 2 hexafluorophosphate is shown in Figure 7, and the peak data are listed in Table 3. [Table 3-1] [Table 3-2]

[0559] Differential Scanning Calorimetry (DSC): DSC was performed on a TA Instruments Discovery DSC2500 differential scanning calorimeter equipped with an autosampler. DSC instrument conditions were as follows: 20-300 °C at 10 °C / min, Tzero aluminum sample pan and lid, and 50 mL / min nitrogen gas flow. DSC analysis of the compound 2 hexafluorophosphate crystalline sample revealed one endothermic peak with an onset of 231.7 °C and a maximum of 232.7 °C due to melting, and a second endothermic peak with an onset of 241.1 °C and a maximum of 242.1 °C due to decomposition. The DSC thermogram of compound 2 hexafluorophosphate is provided in Figure 8.

[0560] Thermogravimetric Analysis (TGA): TGA was obtained from a TA Instruments Thermogravimetric Analyzer, Discovery TGA 5500, equipped with an autosampler. General experimental conditions for TGA: 25 °C to 300 °C ramp at 10 °C / min, nitrogen purge gas flow rate of 25 mL / min, platinum sample holder. TGA analysis of the compound 2 hexafluorophosphate crystalline sample revealed significant weight loss above 250 °C due to decomposition. The TGA thermogram of compound 2 hexafluorophosphate is provided in Figure 9.

[0561] (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluoroarsenate (Compound 2 hexafluoroarsenate) To a solution of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c, 2.94 g, 10.525 mmol) in water (8.06 mL) was added sodium hexafluoroarsenate (NaAsF, 3.35 g, 15.79 mmol, 1.50 equiv.) at ambient temperature. After stirring at 20-25 °C for 12 h, the slurry was cooled in an ice bath for 2 h. The solid was filtered, washed with cold HO (2 x 25 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluoroarsenate (compound 2 hexafluoroarsenate, 4.51 g, theoretical 4.56 g, 99% yield) as a white solid, which was used in the next reaction without further purification. For compound 2 hexafluoroarsenate: 1 HNMR (400MHz, DMSO-d6) δ12.38(s, 1H), 8.83(s, 1H), 7.97(s, 2H), 7.76-7.57(t , J=2.9Hz, 1H), 6.59-6.36(dd, J=3.2, 1.8Hz, 1H), 3.32(s, 6H), 2.35(s, 6H)ppm; 19 FNMR (DMSO-d6, 376.5MHz) δ -62.16 (quartet, 1 J(AsF)=937.5Hz)ppm;C 13 H 18 F6N5As (MW, 433.23 for hexafluoroarsenate compound 2, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0562] (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluoroantimonate (Compound 2 hexafluoroantimonate) To a solution of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium chloride (compound 2c, 2.94 g, 10.525 mmol) in water (8.06 mL) was added sodium hexafluoroantimonate (NaSbF, 4.08 g, 15.79 mmol, 1.50 equiv.) at ambient temperature. After stirring at 20-25 °C for 12 h, the slurry was cooled in an ice bath for 2 h. The solid was filtered, washed with cold HO (3 x 2 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium hexafluoroantimonate (compound 2 hexafluoroantimonate, 2.61 g, theoretical 5.05 g, 51.7% yield) as a white solid, which was used in the next reaction without further purification. For compound 2 hexafluoroantimonate: 1 HNMR (400MHz, DMSO-d6) δ12.37(s,1H), 8.83(s,1H), 7.98(s,2H), 7.68(s,1H), 6.49(s,1H), 3.32(s, 6H), 2.35(s, 6H)ppm; 19 FNMR(DMSO-d6, 376.5MHz)δ-166.86ppm;C 13 H 18 F6N5Sb (MW, 480.07 for hexafluoroantimonate compound 2, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0563] Example 7: Alternative Preparation of (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium Perchlorate (Compound 2 Perchlorate) Method 1 [ka] Oxalyl chloride (20.0 mL, 228 mmol, 3.04 equiv.) was added slowly to DMF (107 mL, 1378 mmol, 18.4 equiv.) over 15 minutes, maintaining the internal temperature below 50 °C. After the addition, the resulting slurry was cooled to ambient temperature and stirred at ambient temperature for 2 hours. 4-Methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a, 10.0 g, 75 mmol) was added to the slurry at ambient temperature, and the resulting reaction mixture was stirred at ambient temperature for 16 hours, followed by stirring at 50 °C for 5.5 hours. The reaction mixture was cooled to ambient temperature and quenched with ice (60 g). The quenched reaction mixture was concentrated in vacuo to give a residue, which was then dissolved in water (50 mL). Sodium perchlorate (NaClO4, 20.23 g, 165 mmol, 2.2 equiv.) was then added to the aqueous solution at ambient temperature. After the resulting mixture was cooled in an ice bath, sodium hydroxide (NaOH, 7.5 g, 188 mmol, 2.5 equiv.) was slowly added. The solid was collected by filtration, washed with water (30 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium perchlorate (compound 2 perchlorate, 18.7 g, theoretical 25.78 g, 72.5% yield), as a gray solid, which was used in the next reaction without further purification. 1 HNMR(400MHz,DMSO-d6)δ12.50-12.17(s,1H),8.94-8.73(s,1H),8.08-7.87(s,2H),7.77-7.57(d C 13 H 18 ClNO (MW, 343.77 for compound 2 perchlorate, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0564] Method 2 [ka] To a solution of 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetic acid (compound 5a, 354 mg, 2.0 mmol) in anhydrous DMF (2.92 g, 3.1 mL, 40 mmol, 20 equiv.) was added phosphorus oxychloride (POCl3, 920 mg, 0.56 mL, 6.0 mmol, 3.0 equiv.) at ambient temperature. The resulting reaction mixture was then warmed to 80-90 °C and stirred at 80-90 °C for 30 min. Upon completion of the reaction, the reaction mixture was cooled to ambient temperature. The cooled reaction mixture was quenched by pouring it onto ice (10 g). The solution was then concentrated under reduced pressure, and the resulting residue was treated with water (3 mL). The aqueous solution was neutralized to pH 7-8 with aqueous NaOH and then treated with activated carbon (50 mg). The mixture was stirred at ambient temperature for 30 min and then filtered through a Celite bed. The Celite bed was washed with water (2 mL). The combined filtrate and washings were then treated with solid sodium perchlorate (NaClO4, 367 mg, 3.0 mmol, 1.5 equiv.) at ambient temperature. The mixture was stirred at ambient temperature for 1 hour, then at 0-5 °C for 1 hour. The solid was then collected by filtration, washed with water (2 × 2 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium perchlorate (Compound 2 perchlorate), 330 mg (theoretical 688 mg, 48% yield) as a gray solid, which was used in the next reaction without further purification. For Compound 2 perchlorate: 1 HNMR (400MHz, DMSO-d6) δ12.50-12.17(s, 1H), 8.94-8.73(s, 1H), 8.08-7.87(s, 2H), 7.77-7.57(d C 13 H 18 ClNO (MW, 343.77 for compound 2 perchlorate, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M + , base peak).

[0565] Method 3 [ka] To a solution of sodium 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (compound 5b, 1.70 g, 8.54 mmol) in anhydrous DMF (12.48 g, 13.2 mL, 171 mmol, 20 equiv.) was added phosphorus oxychloride (POCl3, 3.93 g, 2.4 mL, 25.6 mmol, 3.0 equiv.) at ambient temperature. The resulting reaction mixture was then warmed to 80-90 °C and stirred at 80-90 °C for 30 min. Upon completion of the reaction, the reaction mixture was cooled to ambient temperature. The cooled reaction mixture was quenched by pouring it onto ice (40 g). The solution was then concentrated under reduced pressure, and the resulting residue was treated with water (10 mL). The aqueous solution was neutralized to pH 7-8 with aqueous NaOH and then treated with activated carbon (200 mg). The mixture was stirred at ambient temperature for 30 min and then filtered through a bed of Celite. The Celite bed was washed with water (5 mL). The combined filtrate and washing solution was then treated with solid sodium perchlorate (NaClO4, 1.57 g, 12.8 mmol, 1.5 equiv.) at ambient temperature. The mixture was stirred at ambient temperature for 1 h and then at 0–5 °C for 1 h. The solid was then collected by filtration, washed with water (2 × 5 mL), and dried under vacuum to give the desired crude product, (E)-N-(3-(dimethylamino)-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)allylidene)-N-methylmethanaminium perchlorate (compound 2 perchlorate, 1.3 g, theoretical 2.94 g, 44.3% yield), as an off-white solid, which was used in the next reaction without further purification. 1 HNMR(400MHz,DMSO-d6)δ12.50-12.17(s,1H),8.94-8.73(s,1H),8.08-7.87(s,2H),7.77-7.57(d C 13 H 18 ClNO (MW, 343.77 for compound 2 perchlorate, 244.32 for compound 2 without anion) LCMS (EI) m / e 244.2 (M+ , base peak).

[0566] Example 8 Preparation of 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonaldehyde ((E)-3-hydroxy-2-(7H-pyrrolo[2,3-d]pyrimidin)-4-yl)acrylaldehyde (Compound 2b) [ka] Oxalyl chloride (12.00 mL, 137 mmol, 3.64 equiv.) was added dropwise to DMF (50 mL, 646 mmol, 17.18 equiv.) while maintaining the internal temperature below 50 °C. The resulting mixture was stirred at ambient temperature for 30 minutes. 4-Methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a, 5.00 g, 37.6 mmol) was added as a solid in one portion, and the resulting reaction mixture was stirred at room temperature for 3 days and at 50 °C for 4 hours. Upon completion of the reaction, the reaction mixture was cooled to room temperature and quenched with ice (30 g). Sodium hydroxide (NaOH, 16.1 g, 403 mmol, 10.72 equiv.) was added to the quenched reaction mixture, and the mixture was stirred at room temperature for 26 hours. Additional sodium hydroxide (NaOH, 2.2 g, 55.0 mmol, 1.46 equiv.) was added, and the mixture was stirred at 40 °C for 4 hours. Upon completion of the hydrolysis reaction, the mixture was cooled to 0-5 °C in an ice batch and then concentrated HCl solution was added to adjust the pH to 5-6. The mixture was slowly warmed to ambient temperature and stirred at ambient temperature for 2 h. The solid was collected by filtration, washed with cold water, and dried under vacuum to give the desired crude product, 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonaldehyde ((E)-3-hydroxy-2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acrylaldehyde (compound 2b, 6.33 g, theoretical 7.113 g, 89% yield), as a gray powder, which was used in the next reaction without further purification. For compound 2b: 1HNMR (400MHz, DMSO-d6) δ13.74(brs, 2H), 9.52(s, 2H), 8.73(s, 1H), 7.53(dd, J=3.4, 2 .3Hz, 1H), 7.46(dd, J=3.5, 1.7Hz, 1H)ppm;C9H7N3O2(MW, 189.17)LCMS(EI)m / e190.1(M + , base peak).

[0567] Example 9 Preparation of 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (Compound 12a) [ka] Step 1. 4,6-Dichloropyrimidine-5-carbaldehyde (compound 9a): In a 5 L four-neck flask equipped with a mechanical stirrer, addition funnel, condenser, thermocouple, and a N2 sweep through the aqueous NaOH scrubbing solution, phosphorus oxychloride (POCl3, 1 L, 10.572 mol, 4.82 equiv.) was charged and cooled in an ice / salt bath. N,N-dimethylformamide (DMF, 320 mL, 4.138 mol, 1.85 equiv.) was then added dropwise to the flask at 0 ± 2 °C. After approximately 100 mL of DMF was added over approximately 0.5 h, crystallization occurred and the reaction temperature increased from 0 to 10 °C. The addition was stopped, and the mixture was cooled again to approximately 2 °C. The remaining DMF was added over 2.5 h at below 8 °C. The suspension became very thick, making stirring difficult. When the DMF addition was complete, the mixture was stirred at 3–5 °C for 0.5 h. 4,6-Dihydroxypyrimidine (compound 8a, 250 g, 2.232 mol) was added portionwise as a solid. After approximately one-third of the 4,6-dihydroxypyrimidine was added, the reaction mixture became more fluid and a slow exotherm occurred, increasing the reaction temperature to approximately 12°C over 0.5 hours. The remaining 4,6-dihydroxypyrimidine was added portionwise over 0.25 hours, increasing the reaction temperature from 12 to 27°C. The reaction temperature was maintained at 25-27°C with intermittent cooling, during which time the yellow suspension decreased in viscosity and then thickened once again. After the exotherm subsided in approximately 1 hour, the reaction mixture was slowly heated. At approximately 55°C, the reaction mixture became very thick and a second, mild exotherm occurred. The heating mantle was removed, but the reaction temperature continued to increase to approximately 63°C, remained at this temperature for several minutes, and then decreased. Heating of the mixture was resumed until a gentle reflux (approximately 100°C) was achieved. At approximately 95°C, a steady and fairly rapid evolution of HCl gas began, and the reaction mixture gradually became less viscous and darker. After approximately 0.5 h, a clear brown solution resulted, and the reflux temperature was slowly increased to 115°C over 1.25 h. After a total of 2.5 h of reflux, the reaction mixture was cooled to ambient temperature and stirred at ambient temperature overnight. Excess POCl3 (as much as possible) was removed under reduced pressure (bath temperature 45-50°C).The thick residual brown oil was very slowly poured into cold HO (5 L) in a 20 L separatory funnel, and ice was added as needed to maintain the aqueous mixture near room temperature. The aqueous mixture was extracted with EtOAc (2 × 3 L, followed by 1 × 2 L). The combined EtOAc extracts were washed with HO (2 × 2.5 L), saturated aqueous NaHCO (1 L), brine (1 L), dried over NaSO, filtered, and concentrated under reduced pressure (bath temperature 35 °C) to give crude 4,6-dichloropyrimidine-5-carbaldehyde (compound 9a, 270 g, theoretical 395 g, 68.4%) as a yellow-orange solid. A 20 g aliquot of this crude material was purified by Kugelrohr distillation (90-100 °C oven temperature, 225 mTorr) to give 15.3 g of pure 4,6-dichloropyrimidine-5-carbaldehyde (compound 9a) as a white solid that turned yellow on standing at room temperature. 1 HNMR (300MHz, CDCl3) δ10.46(s,1H), 8.89(s,1H)ppm.

[0568] Step 2. 4-Amino-6-chloropyrimidine-5-carbaldehyde (compound 10a): A solution of 7 M NH in MeOH (265 mL, 1.855 mol, 2.0 equiv.) was added to a solution of 4,6-dichloropyrimidine-5-carbaldehyde (compound 9a, 163.7 g, 0.9301 mol) in toluene (3 L) at ambient temperature over 1.25 h. The reaction temperature slowly increased from 20 to 26 °C, and a yellow suspension formed. Gentle cooling was applied to maintain the reaction temperature below 26 °C. The suspension was stirred at ambient temperature for 3.5 h, after which the solid was collected by filtration. The solid was washed with EtOAc (1 L). The filtrate was concentrated under reduced pressure, and the solid was triturated with toluene and n-heptane (2:1 v / v, 600 mL), filtered, and dried to give 71.1 g of 4-amino-6-chloropyrimidine-5-carbaldehyde as a yellow solid. The original solid filtered from the reaction mixture contained an additional amount of 4-amino-6-chloropyrimidine-5-carbaldehyde. The product was extracted from the filtered solid by stirring in EtOAc (1.25 L) for 1.5 hours, filtering, and then stirring in THF (750 mL) for 1 hour and filtering. Both the EtOAc and THF filtrates were concentrated under reduced pressure, and the resulting solid was triturated with toluene and n-heptane (2:1 v / v, 450 mL), filtered, and dried to give an additional 44.1 g of 4-amino-6-chloropyrimidine-5-carbaldehyde as a yellow solid. The overall yield of 4-amino-6-chloropyrimidine-5-carbaldehyde (115.2 g, theoretical 146.5 g) was 78.6%. For 4-amino-6-chloropyrimidine-5-carbaldehyde: 1 HNMR(300MHz,DMSO-d6)δ10.23(s,1H),8.71(bs,1H),8.55(bs,1H),8.39(s,1H)ppm;C5H4ClN3O(molecular weight 157.56),LCMS(EI)m / e158(M + +H).

[0569] Step 3. 6-chloro-5-(2-methoxyvinyl)pyrimidin-4-ylamine (compound 11a): A suspension of (methoxymethyl)triphenylphosphonium chloride (276.0 g, 0.807 mol, 1.1 equiv.) in THF (1.5 L) was cooled to −2° C. in an ice / salt bath and 1 M potassium tert-butoxide (KO) in THF was added. tBu) (807 mL, 0.807 mol, 1.1 equiv.) was added over 1.5 h at −2 to −3 °C. The deep red to orange mixture was stirred at −2 to −3 °C for 1 h. 4-Amino-6-chloropyrimidine-5-carbaldehyde (Compound 10a, 115.2 g, 0.7338 mol, 1.0 equiv.) was then added portionwise to the reaction mixture as a solid, using THF (200 mL) to rinse the vessel and funnel. During the addition, the reaction temperature increased from −3 to 13 °C, and a brown color developed. When the reaction temperature dropped to 10 °C, the cooling bath was removed, and the reaction mixture was allowed to warm to ambient temperature and stirred at ambient temperature for 42 h. The reaction mixture was cooled to −2 °C and then quenched by the slow addition of saturated aqueous NH4Cl (750 mL). The mixture was concentrated under reduced pressure to remove most of the THF. The residue was partitioned between EtOAc (3 L) and H2O (1 L). The organic phase was filtered to remove insoluble material at the interface and then extracted with 2 N HCl (4 × 250 mL) followed by 3 N HCl (2 × 250 mL). The combined HCl extracts were back-extracted with EtOAc (500 mL) and then filtered through Celite to remove insoluble material. The filtrate was cooled in an ice / brine bath, adjusted to pH 8 with 6 N aqueous NaOH, and extracted with EtOAc (3 × 1 L). The combined EtOAc extracts were washed with brine (1 L), dried over Na2SO4, and stirred with activated carbon (10 g) and silica gel (10 g) for 1 h. The mixture was filtered through Celite, and the Celite pad was washed with EtOAc (1 L). The filtrate was concentrated, and the residual EtOAc was co-evaporated with n-heptane (500 mL). The resulting brown solid was pumped under high vacuum for 2 hours to give crude 6-chloro-5-(2-methoxyvinyl)pyrimidin-4-ylamine (compound 11a, 72.3 g, theoretical 136.2 g, 53.1%). The crude desired product, compound 11a, was used in the following reaction without further purification.A sample of the crude product, Compound 11a (2.3 g), was purified by silica gel column chromatography eluting with 0–35% EtOAc / n-heptane to give 1.7 g of pure 6-chloro-5-(2-methoxyvinyl)pyrimidin-4-ylamine (Compound 11a) as a white solid, which was found to be a 1:2 mixture of E / Z isomers. 1 H NMR (300 MHz, DMSO-d) for E isomer: δ 8.02 (s, 1H), 7.08 (bs, 2H), 6.92 (d, 1H, J = 13.1), 5.35 (d, 1H, J = 13.0 Hz), 3.68 (s, 3H) ppm and for Z isomer: δ 8.06 (s, 1H), 7.08 (bs, 2H), 6.37 (d, 1H, J = 6.8 Hz), 5.02 (d, 1H, J = 6.7 Hz), 3.69 (s, 3H) ppm; C7H8ClNO3 (molecular weight, 185.61), LCMS (EI) m / e 186 / 188 (M + +H).

[0570] Step 4. 4-Chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a): Concentrated aqueous hydrochloric acid (HCl, 5 mL) was added to a solution of crude 6-chloro-5-(2-methoxyvinyl)pyrimidin-4-ylamine (Compound 11a, 70.0 g, 0.3784 mol) in THF (700 mL), and the resulting reaction mixture was heated to reflux for 7.5 hours. Upon warming, a light suspension formed, which gradually redissolved. When the reaction was deemed complete as monitored by HPLC, the reaction mixture was cooled to ambient temperature and stirred at ambient temperature overnight. Solid NaHCO (15 g) was added to the reaction mixture, and the resulting mixture was stirred at ambient temperature for 1 hour. Charcoal (7 g), silica gel (7 g), and NaSO (20 g) were added, and the mixture was heated to 40 °C for 1 hour. The mixture was then cooled to ambient temperature, filtered through Celite, and the Celite pad was washed with THF (1 L). The filtrate was concentrated under reduced pressure, and the resulting solid was dried under reduced pressure to give crude 4-chloro-7H-[pyrrolo[2,3-d]pyrimidine (compound 12a, 58.1 g, theoretical 58.1 g, 100%) as a tan solid. The crude desired product, compound 12, was dissolved in EtOAc (1.0 L) at 50-55 °C and treated with activated charcoal (3 g). The mixture was filtered through Celite while warm, and the Celite pad was washed with warm EtOAc (250 mL). The filtrate was concentrated to approximately 500 mL, and the suspension was allowed to stand at ambient temperature overnight. The suspension was subsequently cooled to 0-5 °C for 2 h, after which the solid was collected by filtration. The solid was dried to give pure 4-chloro-7H-[pyrrolo[2,3-d]pyrimidine (compound 12a, 54.5 g, theoretical 58.1 g, 94%) as tan crystals. For compound 12a: 1 HNMR (400MHz, DMSO-d6) δ12.58(bs, 1H), 8.58(s, 1H), 7.69(d, 1H, J=3.5Hz), 6.59(d, 1H, J=3.5Hz)ppm; LCMS(EI)m / e154 / 156(M + +H).

[0571] Example 10: Alternative preparation of 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a) [ka]

[0572] Step 1. Ethyl 2-cyano-4,4-diethoxybutanoate (compound 14a): A mixture of ethyl cyanoacetate (compound 13a, 182 kg, 1609 mol) and DMSO (325 kg) was added with sodium tert-amyloxide ( t AmONa (158.8 kg) was added portionwise at 5°C. The mixture was then warmed to 70-75°C and ethyl cyanoacetate (191 kg, 1689 mol; total 3298 mol, 5.0 equiv.) was charged. The mixture was stirred at 70-75°C for 30 minutes, after which bromoacetaldehyde diethyl acetal (130.4 kg, 665.2 mol) was added. The resulting reaction mixture was then heated to 90°C and stirred at 90°C until the reaction was complete. The reaction mixture was cooled to 5°C, and a 16% aqueous solution of ammonium chloride (NH4Cl) was added. The mixture was stirred for 30 minutes, after which ethyl acetate (490 kg) was charged. The organic phase was separated and washed with water (695 kg). The aqueous phase was extracted with ethyl acetate (455 kg). The combined organic phases were washed with 17% aqueous sodium chloride solution (NaCl, 318 Kg) and brine (325 Kg). The organic solution was dried over sodium sulfate (NaSO) and filtered. The filtrate was concentrated under reduced pressure. The residue was dissolved in petroleum ether (390 Kg) and treated with charcoal at 60°C. The mixture was filtered, and the filtrate was concentrated to dryness to give crude ethyl 2-cyano-4,4-diethoxybutanoate (compound 14a, 146.6 Kg, theoretical 152.5 Kg, 96.1%) as a yellow to brown oil, which was used directly in the next reaction without further purification.

[0573] Step 2. 7H-Pyrrolo[2,3-d]pyrimidin-4-ol (compound 15a): A reactor was charged with a solution of 18% sodium ethoxide (EtONa) and formamidine acetate (153.5 kg, 1474.4 mol) in ethanol (1558 kg). The mixture was stirred at ambient temperature for 1 hour, and then ethyl 2-cyano-4,4-diethoxybutanoate (14a, 269.8 kg, 1176.7 mol, 1.25 equivalents) was charged. The reaction mixture was heated to 75 °C and stirred at 75 °C until no unreacted ethyl 2-cyano-4,4-diethoxybutanoate (14) was detected. The mixture was cooled to 0 °C and charged with 21% aqueous ammonium chloride (NH4Cl, 783 kg). The resulting mixture was stirred at 0 °C for 30 minutes and concentrated under reduced pressure. The remaining solution was cooled to 20-30 °C and filtered. The cake was reslurried with water (493 kg) and filtered. The solid was suspended in water (474 ​​kg) and concentrated hydrochloric acid (HCl, 89.2 kg) was added. The mixture was stirred at 20°C for 1 hour and then warmed to 30°C until the cyclization reaction was complete. The mixture was then cooled to 5°C and an aqueous solution of ammonium hydroxide (NHOH, 72 kg) was added. After the addition, the mixture was stirred at 5°C for 1 hour and then filtered. The wet cake was washed with water and dried in a vacuum oven to give 7H-pyrrolo[2,3-d]pyrimidin-4-ol (compound 15a, 99.6 kg, theoretical 159 kg, 62.6%) as an off-white to yellow solid, which was used in the next reaction without further purification.

[0574] Step 3. 4-Chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a): 7H-Pyrrolo[2,3-d]pyrimidin-4-ol (compound 15a, 99.6 kg, 737 mol) was added to a solution of DIEA (128.4 kg, 99.53 mol, 1.35 eq) in toluene (500 kg) at ambient temperature, and the resulting mixture was cooled to 0 °C. POCl (338 kg, 2202 mol, 3.0 eq) was then added to the mixture at 0 °C, and the resulting reaction mixture was heated to 70 °C and stirred at 70 °C until the reaction was complete. The reaction mixture was cooled to 30 °C, and water (3500 kg), sodium carbonate (NaCO, 700 kg), and 2-methyltetrahydrofuran (MeTHF, 1200 kg) were added. The reaction mixture was then filtered. The organic phase of the filtrate was separated, washed with brine (424 kg), dried over sodium sulfate (NaSO), and filtered. The filtrate was concentrated to remove approximately 1000 kg of MeTHF. The remaining solution was treated with charcoal (28 kg) at 60° C. for 1 hour and filtered. The filtrate was concentrated to a thick slurry, cooled to 0° C., and filtered. The cake was dried under reduced pressure to give pure 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a, 71.9 kg, theoretical 113.2 kg, 63.5%) as yellow to brown crystals. The 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a) produced by this synthetic method is identical in all respects to the compound obtained by Example 9. 1 HNMR (400MHz, DMSO-d6)12.58(bs, 1H), 8.58(s, 1H), 7.69(d, 1H, J=3.5Hz), 6.59(d, 1H, J=3.5Hz)ppm; LCMS(EI)m / e154 / 156(M + +H).

[0575] Example 11. Preparation of 4-methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a) [ka] A suspension of sodium hydride (NaH, 60% suspension in mineral oil, 309, 7726 mmol, 1.211 equiv.) in THF (4.0 L) was cooled to 0-5 °C in an ice bath and then charged with 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (compound 12a, 980.0 g, 6381 mmol). The mixture was stirred at 0-15 °C for 30 min, and then a THF solution of TBDMS-Cl (1165 g, 7728 mmol, 1.211 equiv.) was charged at 0-15 °C. The resulting mixture was stirred at 0-15 °C for 1-2 h. The mixture was cooled to -10 °C, and iron(III) acetylacetonate (Fe(acac)3, 113 g, 319 mmol, 0.05 equiv.) was added. A solution of methylmagnesium bromide in THF (3260 mL, 9780 mmol, 1.53 equiv.) was slowly charged to the mixture, controlling the internal temperature below 15 °C. The resulting reaction mixture was stirred at 15–30 °C for 2 h. Upon completion of the coupling reaction, the reaction mixture was quenched by charging aqueous ammonium chloride (NH4Cl, 8.0 L), controlling the internal temperature below 10 °C during the quench. The quenched reaction mixture was charged with methyl tert-butyl ether (MTBE, 5.0 L), and the resulting mixture was filtered through a Celite bed. The Celite bed was washed with MTBE (2 × 500 mL). The combined filtrate and washings were separated, and the aqueous phase was extracted with MTBE (2 × 5.0 L). The combined organic extracts were concentrated under reduced pressure, and the residue was dissolved in methanol (MeOH, 5.0 L). This solution was then treated with 26-28% aqueous ammonium hydroxide (NHOH, 1.0 L), and the resulting mixture was stirred at 15-40 °C for 16 h. Upon completion of the N-TBDMS-deprotection reaction, the reaction mixture was concentrated under reduced pressure and charged with n-heptane (2 x 4.0 L) to remove water under azeotropic conditions. The residue was then treated with n-heptane (8.0 L), and the resulting mixture was stirred at ambient temperature for at least 1 h. The solid was collected by filtration and washed with n-heptane (2 x 1.0 L) to afford the desired crude product, 4-methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a, 840 g, theoretical yield 849.6 g), as a brown powder, which was purified by recrystallization in a mixture of ethyl acetate and n-heptane.

[0576] A solution of crude methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a, 1640 g) in methanol (MeOH, 8.0 L) was treated with charcoal (2.0 kg), and the resulting mixture was stirred at ambient temperature for 16 hours. The mixture was filtered through a Celite bed, and the Celite bed was washed with MeOH (2 × 8.0 L). The combined methanol solution was concentrated under reduced pressure, and ethyl acetate (8.0 L) was added to the residue. The resulting solution was concentrated under reduced pressure to remove most of the ethyl acetate (approximately 6.0 L), and then n-heptane (8.0 L) was introduced. The resulting mixture was then stirred at ambient temperature for 14 hours. The solid was collected by filtration, washed with a mixture of ethyl acetate and n-heptane, followed by n-heptane, and dried to constant weight to give purified methyl-7H-pyrrolo[2,3-d]pyrimidine (compound 1a, 1325 g, theoretical 1640 g, 80.8% purified by recrystallization, 80% overall) as a yellow to light brown crystalline powder. For compound 1a: 1 HNMR (DMSO-d6, 500MHz) δ12.10(brs, 1H), 8.61(s, 1H), 7.47(dd, J=3.3, 2.5Hz, 1H), 6.62(s, dd, J=3.5, 1.7Hz, 1H), 2.64(s, 3H)ppm; 13 C NMR (DMSO-d6, 125MHz) δ158.7, 151.3, 151.2, 126.5, 117.6, 99.6, 21.3ppm; C7H7N3 (MW, 133.15) LCMS (EI) m / e134.1 (M + +H, base peak).

[0577] Example 12. Alternative Preparation of 4-Methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 1a) [ka]

[0578] Step 1. 4-Methyl-7H-pyrrolo[2,3-d]pyrimidine (compound 1a): A cloudy mixture of potassium tert-butoxide (18.31 g, 163 mmol, 2.12 equiv.) in THF (100 mL) was cooled in an ice bath and then charged with a solution of 4,4-dimethoxybutanenitrile (compound 16a, 10.00 g, 77 mmol) and ethyl acetate (7.46 g, 85 mmol, 1.1 equiv.) in THF (20 mL) over 15 min. The mixture was allowed to warm to room temperature and stirred at ambient temperature for 3 h. The in situ generated 2-acetyl-4,4-dimethoxybutanenitrile was then treated with formamidine acetate (65.0 g, 624 mmol, 8.1 equiv.), 1-butanol (80 mL), and triethyl orthoformate (56.2 mL, 337 mmol, 4.38 equiv.) at ambient temperature. The resulting mixture was heated to 110-120 °C and stirred at 110-120 °C for 1 h. Additional triethyl orthoformate (26.5 mL, 159 mmol, 2.06 equiv) was added. The mixture was stirred at 110° C. for an additional 16 h. Additional formamidine acetate (31.38 g, 302 mmol, 3.92 equiv) and triethyl orthoformate (56.5 mL, 115 mmol, 1.5 equiv) were added in three portions over 24 h. The mixture was heated for an additional 24 h and concentrated under reduced pressure to give a residue. The residue was treated with water (150 mL) and MeTHF (210 mL). The resulting mixture was passed through a bed of Celite (12 g). The two phases of the filtrate were separated, and the aqueous phase was extracted with MeTHF (175 mL×2). The combined organic extracts were concentrated under reduced pressure, and the resulting residue was treated with an HCl solution in IPA (5.5 M, 50.8 g), water (31 mL), and concentrated HCl (12 M, 15.6 g). The mixture was stirred at room temperature for 3 days. Concentrated aqueous NH4OH (38.6 g, 28-30%) was added, and the mixture was concentrated to give a residue that was triturated with THF (170 mL, 2 × 150 mL). The combined filtrates were concentrated to give a residue that was dissolved in DCM (30 mL) and purified by column chromatography on silica gel (SiO2, 120 g) eluting with 0-100% EtOAc in DCM to give the desired product, 4-methyl-7H-pyrrolo[2,3-d]pyrimidine (compound 1a, 5.1 g, theoretical yield 10.25 g, 49.8% over three steps), as an off-white crystalline solid, which is identical in all respects to the compound obtained in Example 11.

[0579] Step 2. 2-Acetyl-4,4-dimethoxybutanamide (compound 20a): A solution of 3-oxobutanamide (compound 19a, 5.0 g, 49.5 mmol) in DMF (15 mL) was treated with cesium carbonate (CsCO, 16.11 g, 49.5 mmol, 1.0 equiv.) at ambient temperature. The resulting mixture was stirred at ambient temperature. 2-Bromo-1,1-dimethoxyethane (8.36 g, 49.5 mmol, 1.0 equiv.) was then added to the mixture, and the resulting reaction mixture was heated to 80 °C for 5–8 h. The reaction mixture was cooled to ambient temperature and then quenched with water (20 mL). The quenched reaction mixture was then extracted with ethyl acetate (3 × 20 mL), and the combined organic extracts were washed with water (2 × 10 mL), dried over anhydrous sodium sulfate (NaSO), and concentrated under reduced pressure. The residue was purified by silica gel (SiO2) column chromatography to give 2-acetyl-4,4-dimethoxybutanamide (compound 20a, 5.8 g, theoretical value 9.37 g, 61.9%) as a viscous oil containing some residual DMF. 1 C8H 15 NO4(MW, 189.21), LCMS(EI)m / e190.2(M + +H).

[0580] Step 3. 2-Acetyl-4,4-dimethoxybutanenitrile (Compound 17a): A solution of 2-acetyl-4,4-dimethoxybutanamide (compound 20a, 1.0 g, 4.23 mmol) in DMF (4 mL) was treated with cyanuric chloride (compound 21a, 0.39 g, 2.11 mmol, 0.5 equivalents). The resulting reaction mixture was stirred at ambient temperature for 1 hour. Upon completion of the reaction, the reaction mixture was quenched with water (10 mL), and the quenched reaction mixture was extracted with ethyl acetate (3 × 10 mL). The combined organic extracts were washed with water (2 × 10 mL), dried over anhydrous sodium sulfate (NaSO), and concentrated under reduced pressure. The residue was purified by silica gel (SiO) column chromatography to give 2-acetyl-4,4-dimethoxybutanenitrile (compound 17a, 280 mg, theoretical yield 724 mg, 38.7%) as a viscous oil. For 2-acetyl-4,4-dimethoxybutanenitrile: 1 H NMR (DMSO-d6, 400 MHz, mixture of ketone and enol forms obtained): δ 10.7 (br.s, 1 / 2H for enol form at -OH), 4.38 (m, 1H), 3.25 (m, 6H for two OMe, 1 / 2H for ketone form at -CH-), 2.25-2.50 (m, 2H), 2.15 and 2.25 (s, 3H); CH 13 NO3(MW, 171.196), LCMS(EI)m / e172.2(M + +H). The 2-acetyl-4,4-dimethoxybutanenitrile (compound 17a) produced in this manner was reacted with formamidine acetate, followed by treatment with HCl to give 4-methyl-7H-pyrrolo[2,3-d]pyrimidine (compound 1a), as described in Example 11 above.

[0581] Example 13. Preparation of 4-methyl-(7H-pyrrolo[2,3-d]pyrimidin-4-yl) hydrochloride (Compound 1a hydrochloride) [ka] A reactor under nitrogen was charged with 2,4-dichloro-7H-pyrrolo[2,3-d]pyrimidine (Compound 22a, 200 g, 1.064 mol) and THF (1.2 L). After cooling the reactor contents to below −5°C, 60% NaH in mineral oil (51 g, 1.28 mol, 1.2 equiv.) was added portionwise. The internal temperature was maintained between −5 and 5°C during the NaH addition. Stirring was continued for 30 min after the addition, and then TBDMS-Cl (193 g, 1.28 mol, 1.2 equiv.) in THF (200 mL) was added slowly, maintaining the internal temperature between −5 and 5°C. Stirring of the reaction mixture was continued for 30 min, followed by the addition of Fe(acac)3 (18.8 g, 53.2 mmol, 0.05 equiv.), followed by the addition of 3.0 M MeMgCl solution in THF (532 mL, 1.596 mol, 1.5 equiv.) at -5 to 5 °C. After holding the reaction mixture for an additional 1 h, IPC by HPLC indicated the coupling reaction was complete. The reaction mixture was poured into a solution of EDTA disodium salt dihydrate (200 g) in water (2.0 L) while controlling the internal temperature below 15 °C. The biphasic mixture was diluted with methyl tert-butyl ether (MTBE, 2.0 L), treated with Celite (150 g), and filtered in a centrifuge. The solid cake was washed with MTBE, and the filtrate was phase-separated. The aqueous layer was separated and extracted with MTBE (1.0 L). The organic phases were combined and washed successively with 3% aqueous citric acid (2 × 400 mL) and brine (600 mL). After drying over NaSO, the organic phase was filtered and concentrated to dryness. The residue was taken up in petroleum ether (2.0 L), and insoluble material was removed by filtration through a thin layer of silica gel. The filtrate was concentrated to give the desired crude product, 7-(tert-butyldimethylsilyl)-2-chloro-4-methyl-7H-pyrrolo[2,3-d]pyrimidine (Compound 22a, 300 g), as an oil, which was used directly in the next reaction without further purification.

[0582] A mixture of crude 7-(tert-butyldimethylsilyl)-2-chloro-4-methyl-7H-pyrrolo[2,3-d]pyrimidine (compound 22a, 300 g, 1.064 mol) and 5% palladium on carbon (Pd / C, 30 g) in methanol (1.8 L) was vigorously stirred under 1 atmosphere of hydrogen at 50–55 °C for 3 h. After completion of the reaction was confirmed by IPC HPLC, the reaction mixture was cooled to 20–25 °C and filtered. The filter cake was washed with methanol, and the filtrate was concentrated to dryness. The residue was suspended in ethyl acetate (EtOAc, 225 mL) and stirred at 10–15 °C for 1 h. The solid was collected by filtration, washed with ethyl acetate, and dried under vacuum at 40-45°C to give 4-methyl-7H-pyrrolo[2,3-d]pyrimidine hydrochloride (compound 1a hydrochloride, 151.5 g, theoretical 180.5 g, 84% yield over two steps) as a pale yellow crystalline powder. 1 HNMR (DMSO-d6, 500MHz) δppm13.54(brs, 1H), 9.04(s, 1H), 7.95(dd, J=3.4, 2.4Hz, 1H), 7.13(s, dd, J=3.4, 1.5Hz, 1H), 2.97(s, 3H); 13 C NMR (DMSO-d6, 125 MHz) δ ppm 154.0, 151.0, 144.0, 131.6, 117.2, 103.1, 17.6; C7H8ClN3 (MW, 169.61; for C7H7N3 free base, MW 133.15); LCMS (EI) m / e 134.1 (M + +H, base peak).

[0583] Example 14. Sodium 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (5b) and 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetic acid (compound 5a) [ka]

[0584] Step 1. 4-Chloro-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine (compound 24a): To a suspension of 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (Compound 12a, 18.0 g, 117 mmol) in acetone (180 mL) was added 50% aqueous sodium hydroxide (NaOH, 14.07 g, 176 mmol, 1.5 equiv.) at ambient temperature. The resulting mixture was then stirred at ambient temperature until a clear solution was formed. p-Toluenesulfonyl chloride (pTsCl, 25.7 g, 135 mmol, 1.15 equiv.) was added to the solution at ambient temperature, and the resulting reaction mixture was stirred at ambient temperature for 1 hour. Upon completion of the reaction, the reaction mixture was filtered and the solid was washed with acetone before being discarded. The filtrate was then concentrated under reduced pressure, and the residue was treated with methyl tert-butyl ether (MTBE, 180 mL) and n-heptane (180 mL). The resulting mixture was stirred at ambient temperature for 1 hour. The solid was collected by filtration, washed with n-heptane (180 mL), and dried in a vacuum oven to constant weight to give the desired product, 4-chloro-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine (compound 24a, 32.1 g, theoretical 36.0 g, yield 89.2%) as an off-white powder, which was used in the next reaction without further purification. 1 HNMR (DMSO-d6,400MHz)δ8.78(s,1H),8.10(d,2H),7.79(d,1H),7.34(d,2H),6.72(d,1H),2.41(s,3H)ppm;C 13 H 10 ClN3O2S(MW, 307.75), LCMS(EI)m / e308.1(M + +H).

[0585] Step 2. Diethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonate (Compound 25a): A solution of 4-chloro-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine (compound 24a, 7.0 g, 22.75 mmol) and diethylmalonate (5.46 g, 34.1 mmol, 1.5 equiv.) in anhydrous DMF (30 mL) was treated with solid cesium carbonate (CsCO, 18.53 g, 56.9 mmol, 2.5 equiv.) at ambient temperature. The resulting reaction mixture was then warmed to 50-60 °C and stirred at 50-60 °C for 2-3 h. Upon completion of the reaction, the reaction mixture was cooled to ambient temperature and then treated with water (HO, 80 mL). The quenched reaction mixture was then stirred at ambient temperature for 1 h, followed by 1 h at 0-5 °C. The solid was collected by filtration, washed with water (50 mL) followed by n-heptane (50 mL), and dried to constant weight in a vacuum oven at 40° C. to give the desired product, diethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonate (compound 25a, 6.2 g, theoretical 9.81 g, yield 63.2%), as an off-white powder, which was used in the next reaction without further purification. 1 C 20 H 21 N3O6S(MW, 431.46), LCMS(EI)m / e432.3(M + +H).

[0586] Step 3. Ethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (Compound 26a): A solution of diethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonate (Compound 25a, 4.0 g, 9.27 mmol) in ethanol (EtOH, 20 mL) was treated with a solution of 21% sodium ethoxide in ethanol (NaOEt, 21 wt%, 0.30 g, 0.927 mmol, 0.10 equiv.) at ambient temperature, and the resulting reaction mixture was stirred at ambient temperature for 12 hours. The reaction mixture was quenched with 0.1 N aqueous hydrochloric acid (10 mL), and the resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel (SiO2) column chromatography to obtain the desired product, ethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (compound 26a, 2.08 g, theoretical 3.33 g, yield 62.6%), as an off-white powder, which was used in the next reaction without further purification. 1 C 17 H 17 N3O4S(MW, 359.40), LCMS(EI)m / e360.2(M + +H).

[0587] Step 4. Ethyl 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (Compound 27a): A solution of diethyl 2-(7-tosyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)malonate (compound 25a, 4.0 g, 9.27 mmol) in ethanol (EtOH, 20 mL) was treated with a solution of 21% sodium ethoxide in ethanol (NaOEt, 21 wt%, 3.0 g, 9.27 mmol, 1.0 equiv.) at ambient temperature. The resulting reaction mixture was heated to 65-75 °C and stirred at 65-75 °C for 12 h. The reaction mixture was quenched with 1.0 N aqueous hydrochloric acid, and the resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel (SiO2) column chromatography to obtain the desired product, ethyl 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (compound 27a, 1.3 g, theoretical 1.9 g, yield 68.3%), as an off-white powder, which was used in the next reaction without further purification. In the case of ethyl 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate: 1 C 10 H 11 N3O2(MW, 205.22), LCMS(EI)m / e206.2(M + +H).

[0588] Step 5. Sodium 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (Compound 5b): A solution of ethyl 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (Compound 27a, 1.2 g, 5.85 mmol) in acetone (10 mL) and THF (10 mL) was treated with an aqueous solution of 6N sodium hydroxide (6N NaOH, 1.462 mL, 8.77 mmol, 1.5 equiv.) at ambient temperature. The resulting reaction mixture was stirred at ambient temperature for 5 hours. The solid was collected by filtration, and the isolated solid was suspended in methanol (MeOH, 4.0 mL). Acetone (15 mL) was then added to the resulting suspension, and the mixture was stirred at ambient temperature for 1 hour. The solid was collected by filtration, washed with acetone (2 x 5 mL), and dried under vacuum to give the desired product, sodium 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (compound 5b, 1.1 g, theoretical 1.164 g, 94.5% yield) as an off-white powder, which was used in the next reaction without further purification. 1 HNMR (DMSO-d6, 400 MHz) δ 8.36 (s, 1H), 7.37 (d, 1H), 6.40 (d, 1H), 3.61 (s, 2H) ppm. C8H6N3NaO2 (MW, 199.15; for the corresponding acid, C8H7N3O2, MW 177.16). LCMS (EI) m / e 178.1 (M + +H).

[0589] Step 6. 2-(7H-Pyrrolo[2,3-d]pyrimidin-4-yl)acetic acid (compound 5a): A solution of ethyl 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetate (Compound 27a, 1.2 g, 5.85 mmol) in acetone (10 mL) and THF (10 mL) was treated with an aqueous solution of 6 N sodium hydroxide (6 N NaOH, 1.462 mL, 8.77 mmol, 1.5 equiv.) at ambient temperature. The resulting reaction mixture was stirred at ambient temperature for 5 hours. The reaction mixture was then treated with 1 N aqueous hydrochloric acid (1 N HCl, 9.0 mL) and then concentrated under reduced pressure. The residue was purified by silica gel (SiO2) column chromatography to give the desired product, 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetic acid (Compound 5a, 0.83 g, theoretical 1.04 g, 79.8% yield), as an off-white solid, which was used in the next reaction without further purification. For 2-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)acetic acid: 1 HNMR(DMSO-d6,400MHz)δ12.01(brs,1H),8.56(s,1H)), 7.36(d, 1H), 6.57(d, 1H), 3.66(s, 2H)ppm;C8H7N3O2(MW, 177.16), LCMS(EI)m / e 178.1(M + +H).

[0590] Example 15. Preparation of 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile adipate [ka]

[0591] Step 1. 2-(azetidin-3-ylidene)acetonitrile hydrochloride: To a 0.5 L flask equipped with a nitrogen inlet, a thermocouple, and a mechanical stirrer, tert-butyl 3-(cyanomethylene)azetidine-1-carboxylate (30 g, 154.46 mmol) and methylene chloride (300 mL) were added at ambient temperature. The solution was then treated with a 5 M solution of hydrogen chloride (HCl) in isopropanol (294.2 mL, 1.54 mol, 10 equiv.) at ambient temperature, and the resulting reaction mixture was stirred at ambient temperature for 18 hours. After the reaction was complete as monitored by HPLC, tert-butyl methyl ether (TBME, 150 mL) was added to the suspension, and the mixture was stirred at ambient temperature for 2 hours. The solid was collected by filtration, washed with n-heptane (2×100 mL), and dried on the filter funnel at ambient temperature for 3 hours to give 2-(azetidin-3-ylidene)acetonitrile hydrochloride (13.7 g, theoretical 20.2 g, 67.8%) as a white solid. 1 HNMR(500MHz,DMSO-d6)δ9.99(s,2H),5.94(p,J=2.5Hz,1H),4.85-4.80(m,2H),4.77-4.71(m,2H)ppm; 13 C NMR (126 MHz, DMSO-d) δ 155.65, 114.54, 94.78, 55.26, 54.63 ppm; C5H7ClN2 (MW 130.58; for free base C5H6N2, MW 94.11); LCMS (EI) m / e 95 (M + +H).

[0592] Step 2. 2-(1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-ylidene)acetonitrile: To a 0.25 L flask equipped with a nitrogen inlet, a thermocouple, and a magnetic stirrer, 2-(azetidin-3-ylidene)acetonitrile hydrochloride (4.5 g, 34.46 mmol), 1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-one (10 g, 34.46 mmol, 1.0 equiv.), and methylene chloride (100 mL) were added at ambient temperature, and the resulting mixture was then stirred. Sodium triacetoxyborohydride(14.6 g, 68.93 mmol, 2.0 equiv) at ambient temperature. The reaction mixture was stirred at ambient temperature for 2 hours and then quenched with saturated aqueous sodium bicarbonate (NaHCO) (50 mL). The two phases were separated, and the aqueous phase was extracted with dichloromethane (200 mL). The combined organic phases were washed with water (50 mL) and brine (50 mL) and concentrated under reduced pressure to give the crude desired product, which was purified by column chromatography (SiO, 0-10% ethyl acetate in hexane gradient elution) to give 2-(1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-ylidene)acetonitrile (9.5 g, theoretical 12.7 g, 74.8%) as a white solid. 1 HNMR(400MHz,CDCl3)δ8.57(d,J=4.7Hz,1H),7.54(t,J=4.6Hz,1H),5.29(p,J=2.4Hz,1H),4.18-4.08(m,1H),4.08-4.03(m,2H),3.98-3.94(m,2H) ,3.57-3.39(m,2H),3.17-3.04(m,1H),2.56(tt,J=7.4,3.5Hz,1H),1.86- 1.77(m,1H),1.75-1.64(m,1H),1.54-1.43(m,1H),1.43-1.31(m,1H)ppm; 13 C NMR(101MHz,CDCl3)δ161.34,160.73,152.62(d,J=269.1Hz),145.75(d,J=6.1Hz),136.73(qd,J=36.1,12.0Hz),134.56 (d,J=16.9Hz),126.89,120.58(qd,J=275.0,4.9Hz),115.11,92.04,62.05,60.57(2C),44.47,39.42,29.38,28.47ppm;C 17 H 16 F4N4O(MW368.33),LCMS(EI)m / e369(M + +H).

[0593] Step 3. 2-(1-(1-(3-fluoro-2-(trifluoromethyl)trifluoromethyl)piperidin-4-yl)-3-hydrazinylazetidin-3-yl)acetonitrile: A flask under nitrogen was charged with 2-(1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-ylidene)acetonitrile (1.2 g, 3.26 mmol, 1.0 equiv) and acetonitrile (10 mL). Hydrazine hydrate (0.40 g, 6.84 mmol, 2.1 equiv) was added slowly to the reaction mixture over 30 minutes while controlling the reaction temperature below 25° C. The reaction was stirred at ambient temperature for 5 hours to reach completion. After completion of the reaction, the reaction solvent was evaporated under reduced pressure. The residual reaction mixture was diluted with DCM (10 mL) and washed with brine (5 mL). The organic layer was separated and collected. The aqueous layer was extracted with another DCM (10 mL) and the organic layer was collected. The combined organic layers were evaporated under reduced pressure. The desired crude product, 2-(1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)-3-hydrazinylazetidin-3-yl)acetonitrile (0.52 g, 37%), was obtained as a pale yellow gel, which was used directly in the next step without further purification. 1 HNMR (DMSO-d6, 400MHz) δ8.66(d, J=4.7Hz, 1H), 7.89(t, J=4.7Hz, 1H), 4.10-3.99(m, 1H), 3.91(s, 1H), 3.52-3.20(m, 4H), 3.11-2.94(m, 5H), 2.90(s, 2H), 2.38(dt, J=8.2, 4.6Hz, 1H), 1.76-1.64(m, 1H), 1.63-1.51(m, 1H), 1.35-1.01(m, 2H)ppm; 13 C NMR (DMSO-d6, 101 MHz) δ 160.20, 152.13 (153.45, 150.81), 146.22 (146.25, 146.19), 134.70 (134.79, 134.62), 127.53, 120.60 (121.99, 121.94, 119.28, 119.26), 118.98, 61.41, 58.75, 55.83, 44.20, 28.84, 28.08, 24.28 ppm; only peaks are shown; C17 H 20 F4N6O(MW400.38), LCMS(EI)m / e401.3(M + +H), 423.3(M + +Na).

[0594] Step 4. 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile: To a solution of 2-(1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)-3-hydrazinylazetidin-3-yl)acetonitrile (0.606 g, 1.51 mmol, 1.30 equiv) in ethanol (3 mL) was added vinamidinium perchlorate (0.40 g, 1.16 mmol) in one portion. The resulting reaction mixture was stirred at ambient temperature for 16 hours. After completion of the reaction, the reaction solvent was evaporated under reduced pressure. The residual reaction mixture was diluted with DCM (10 ml) and washed with brine (5 mL). The organic layer was separated and collected. The aqueous layer was extracted with another portion of DCM (10 mL) and the combined organic layers were evaporated under reduced pressure. The desired crude product, 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile (0.58 g, 95%), was obtained as a yellow gel and used directly in the subsequent salt formation step without further purification. The free base obtained by this synthetic method is identical in all respects to compounds obtained by previously reported synthetic methods (see, e.g., U.S. Publication No. 2011 / 0224190, the disclosure of which is incorporated herein by reference in its entirety). 1HNMR(400MHz,DMSO-d6)δ12.17(d,J=2.8Hz,1H),8.85(s,1H),8.70(m,2H),8.45(s,1H),7.93(t, J=4.7Hz,1H),7.63(dd,J=3.6,2.3Hz,1H),7.09(dd,J=3.6,1.7Hz,1H),4.10(m,1H),3.78(d,J=7 .9Hz,2H),3.61(t,J=7.9Hz,1H),3.58(s,2H),3.46(m,1H),3.28(t,J=10.5Hz,1H),3.09(ddd,J= 13.2,9.5,3.1Hz,1H),2.58(m,1H),1.83-1.75(m,1H),1.70-1.63(m,1H),1.35-1.21(m,2H)ppm; 13 C NMR(101MHz,DMSO-d6)δ160.28,(153.51,150.86),152.20,150.94,149.62,(146.30,1 46.25),139.48,(134.78,134.61),(135.04,134.92,134.72,134.60,134.38,134.26, 134.03,133.92),129.22,127.62,126.84,121.99,122.04,(124.77,122.02,119.19,1 16.52),117.39,113.00,99.99,61.47,60.49,57.05,44.23,28.62,27.88,27.19ppm;C 26 H 23 F4N9O(MW,553.51),LCMS(EI)m / e554.1(M + +H).

[0595] Step 5. 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile adipate: To a 0.5 L flask equipped with a mechanical stirrer, thermocouple, addition funnel, and nitrogen inlet was added a solution of crude 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile (31.38 g, 56.7 mmol) in acetone (220 mL) and adipic acid (8.7 g, 59.53 mmol, 1.05 equiv.) at ambient temperature. The reaction mixture was then heated to reflux to obtain a solution. n-Heptane (220 mL) was added slowly to the reaction mixture at 40-50° C. over 1 hour. The resulting mixture was slowly cooled to ambient temperature over 1 hour and stirred at ambient temperature for an additional 16 hours. The solid was collected by filtration, washed with n-heptane (2 × 60 mL), and dried to constant weight under vacuum at 50°C with a nitrogen sweep to give 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile adipate (34.0 g, theoretical 39.7 g, 85.6% for two steps) as a white to off-white solid. 1HNMR(400MHz,DMSO-d6)δ12.16(s,1H),12.05(brs,2H),8.85(s,1H),8.72(s,1H),8.69(d,J=4.7Hz,1H),8.45(s,1H),7.93(t, J=4.7Hz,1H),7.63(dd,J=3.6,2.3Hz,1H),7.09(dd,J=3.6,1.7Hz,1H),δ4.11(dt,J=11.0,4.4Hz,1H),3.77(d,J=7.8Hz,2H),3. 60(t,J=7.8Hz,2H),3.58(s,2H),3.44(dt,J=14.4,4.6Hz,1H),3.28(t,J=10.4Hz,1H),3.09(ddd,J=13.2,9.6,3.2Hz,1H),2.58 (tt,J=8.6,3.5Hz,1H),2.28-2.17(m,4H),1.83-1.74(m,1H),1.67(d,J=11.0Hz,1H),1.59-1.46(m,4H),1.37-1.21(m,2H)ppm; 13 C NMR(101MHz,DMSO-d6)δ174.38,160.29,(153.52,150.87),152.20,150.94,149.63,(146.3 0,146.25),139.48,(134.79,134.62),(135.08,134.97,134.74,134.62,134.38,134.28,13 4.04,133.93),129.21,127.62,126.84,122.05,(124.75,122.02,119.29,116.54),117.39 ,113.01,99.99,61.47,60.50,57.06,44.24,33.42,30.70,28.63,27.89,27.20,24.07ppm;C 32 H 33 F4N9O5 (molecular weight 699.66; free radical についてC 26 H 23 F4N9O, molecular weight, 553.51), LCMS (EI) m / e 554.0 (M + +H).

[0596] Example 16. Alternative Preparation of 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile Adipate [ka]

[0597] Step 1. tert-Butyl 3-(cyanomethyl)-3-hydrazinylazetidine-1-carboxylate: A reaction vial under nitrogen was charged with tert-butyl 3-(cyanomethylene)azetidine-1-carboxylate-3-ylidene)acetonitrile (2.0 g, 10.3 mmol, 1.0 equiv.) and acetonitrile (10 mL). Hydrazine hydrate (1.26 g, 21.6 mmol, 2.1 equiv.) was added slowly to the reaction mixture over 20 minutes while controlling the reaction temperature below 25° C. The reaction was stirred at ambient temperature for 1 hour. After completion of the reaction, the reaction solvent was evaporated under reduced pressure. The residual reaction mixture was diluted with DCM (10 mL) and washed with brine (5 mL). The organic layer was separated and collected. The aqueous layer was extracted with another 10 mL of DCM, and the combined organic layers were evaporated under reduced pressure. The desired crude product, tert-butyl 3-(cyanomethyl)-3-hydrazinylazetidine-1-carboxylate (INCB125078, 2.40 g, 95%), was obtained as a white solid, which was used directly in the next step without further purification. 1 HNMR (CDCl3, 400MHz) δ3.80(q, J=9.4Hz, 4H), 3.17(br, 3H), 2.90(s, 2H), 1.43(s, 9H)ppm; 13 C NMR (CDCl3, 101MHz) δ156.40, 117.19, 80.34, 57.09, 56.07, 28.46, 24.82ppm;C 10 H 18 N4O2(MW226.28), LCMS(EI)m / e227.3(M + +H).

[0598] Step 2. tert-Butyl 3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-3-(cyanomethyl)azetidine-1-carboxylate: To a solution of tert-butyl 3-(cyanomethyl)-3-hydrazinylazetidine-1-carboxylate (2.40 g, 10.59 mmol, 1.30 equiv.) in ethonal (20 mL) was added vinamidinium perchlorate (INCB122809 perchlorate, 2.80 g, 8.15 mmol) in one portion. The resulting reaction mixture was stirred at ambient temperature for 4 hours. Upon completion, n-heptane (10 mL) was added to the reaction mixture and stirred at ambient temperature for an additional hour. The reaction mixture was filtered, and the solid was washed with n-heptane (10 mL). After drying overnight by passing air through the wet cake, the desired crude product, tert-butyl 3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-3-(cyanomethyl)azetidine-1-carboxylate (3.10 g, 95%), was obtained as a brown solid, which was used directly in the next step without further purification. 1 HNMR (CDCl3, 400MHz) δ10.54(s, 1H), 8.85(s, 1H), 8.48(s, 1H), 8.35(s, 1H), 7.44(d, J=3.4Hz, 1H) , 6.79(d, J=3.5Hz, 1H), 4.55(d, J=9.7Hz, 2H), 4.30(d, J=9.7Hz, 2H), 3.34(s, 2H), 1.47(s, 9H)ppm; 13 C NMR (CDCl3, 101MHz) δ156.00, 152.55, 151.37, 150.40, 140.94, 128.55, 126. 06, 123.05, 115.41, 114.34, 100.67, 81.33, 59.00, 56.99, 28.42, 28.25ppm;C 19 H 21 N7O2(MW379.42), LCMS(EI)m / e380.4(M + +H).

[0599] Step 3. 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)azetidin-3-yl)acetonitrile dihydrochloride: To a 0.5 L flask equipped with a nitrogen inlet, a thermocouple, an addition funnel, and a mechanical stirrer, tert-butyl 3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-3-(cyanomethyl)azetidine-1-carboxylate (15 g, 39.5 mmol), water (7.5 mL, 416 mmol), and dichloromethane (75 mL) were added at room temperature. The mixture was stirred at room temperature to form a suspension. A solution of 5 M hydrogen chloride (HCl) in isopropanol (55 mL, 275 mmol, 7.0 equiv.) was added to the suspension over 5 minutes. The resulting reaction mixture was then heated to a gentle reflux and maintained at reflux for 3-4 hours. After the reaction was complete as monitored by HPLC, tert-butyl methyl ether (TBME, 45 mL) was added to the reaction suspension. The mixture was allowed to cool to room temperature and stirred for an additional hour. The solid was collected by filtration, washed with tert-butyl methyl ether (TBME, 45 mL), and dried to constant weight under vacuum at 50° C. with a nitrogen sweep to give 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)azetidin-3-yl)acetonitrile dihydrochloride (13.6 g, theoretical 13.9 g, 98%) as an off-white to pale yellow solid. 1 HNMR (400MHz, D2O) δ8.96(s, 1H), 8.81(s, 1H), 8.49(s, 1H), 7.78(d, J=3.8Hz, 1H), 7. 09(d, J)=3.7Hz, 1H), 4.93(d, J=12.8Hz, 2H), 4.74(d, J=12.5Hz, 2H), 3.74(s, 2H)ppm; 13 C NMR (101MHz, D2O) δ151.35, 143.75, 143.33, 141.33, 132.03, 131.97, 115.90, 114.54, 113.85, 103.18, 59.72, 54.45(2C), 27.02ppm;C 14 H 15 C l2 N7(C 14 H13 For N7 free base, MW 279.30, LCMS (EI) m / e 280 (M + +H).

[0600] Step 4. 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile: To a 0.5 L flask equipped with a nitrogen inlet, thermocouple, addition funnel, and mechanical stirrer, 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)azetidin-3-yl)acetonitrile dihydrochloride (20 g, 56.78 mmol), dichloromethane (200 mL), and triethylamine (TEA, 16.62 mL, 119.2 mmol, 2.1 equiv.) were added at ambient temperature. The mixture was stirred at ambient temperature for 30 minutes, after which 1-(3-fluoro-2-(trifluoromethyl)-isonicotinoyl)piperidin-4-one (17.15 g, 57.91 mmol, 1.02 equiv.) was added to the mixture. The mixture was then Sodium triacetoxyborohydride(25.34 g, 113.6 mmol, 2.0 equiv.) at ambient temperature (below 26 °C) for 5 minutes. The resulting reaction mixture was stirred at ambient temperature for 2 hours. After the reaction was complete as monitored by HPLC, the reaction mixture was quenched with saturated aqueous NaHCO3 (200 mL). The two phases were separated, and the aqueous phase was extracted with methylene chloride (200 mL). The combined organic phases were washed with 4% brine (100 mL), followed by a solvent switch of methylene chloride to acetone by distillation. The resulting solution of the desired crude product (8) in acetone was used directly for the subsequent adipate salt formation. A small portion of the solution was purified by column chromatography (SiO, 0-10% MeOH in EtOAc gradient elution) to afford analytically pure 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile as an off-white solid. The 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile obtained by this synthetic method is identical in all respects to the compound obtained by Example 15 and previously reported synthetic methods (see, e.g., U.S. Publication No. 2011 / 0224190, the disclosure of which is incorporated herein by reference in its entirety). 1HNMR(400MHz,DMSO-d6)δ12.17(d,J=2.8Hz,1H),8.85(s,1H),8.70(m,2H),8.45(s,1H),7.93(t, J=4.7Hz,1H),7.63(dd,J=3.6,2.3Hz,1H),7.09(dd,J=3.6,1.7Hz,1H),4.10(m,1H),3.78(d,J=7 .9Hz,2H),3.61(t,J=7.9Hz,1H),3.58(s,2H),3.46(m,1H),3.28(t,J=10.5Hz,1H),3.09(ddd,J= 13.2,9.5,3.1Hz,1H),2.58(m,1H),1.83-1.75(m,1H),1.70-1.63(m,1H),1.35-1.21(m,2H)ppm; 13 C NMR(101MHz,DMSO-d6)δ160.28,(153.51,150.86),152.20,150.94,149.62,(146.30,1 46.25),139.48,(134.78,134.61),(135.04,134.92,134.72,134.60,134.38,134.26, 134.03,133.92),129.22,127.62,126.84,121.99,122.04,(124.77,122.02,119.19,1 16.52),117.39,113.00,99.99,61.47,60.49,57.05,44.23,28.62,27.88,27.19ppm;C 26 H 23 F4N9O(MW,553.51),LCMS(EI)m / e554.1(M + +H).

[0601] Step 5. 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile adipate: To a 0.5 L flask equipped with a mechanical stirrer, thermocouple, addition funnel, and nitrogen inlet was added a solution of crude 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile (31.38 g, 56.7 mmol) in acetone (220 mL) and adipic acid (8.7 g, 59.53 mmol, 1.05 equiv.) at ambient temperature. The reaction mixture was then heated to reflux to obtain a solution. n-Heptane (220 mL) was added slowly to the reaction mixture at 40-50° C. over 1 hour. The resulting mixture was slowly cooled to ambient temperature over 1 hour and stirred at ambient temperature for an additional 16 hours. The solid was collected by filtration, washed with n-heptane (2 x 60 mL), and dried to constant weight under vacuum at 50°C with a nitrogen sweep to give 2-(3-(4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl)-1-(1-(3-fluoro-2-(trifluoromethyl)isonicotinoyl)piperidin-4-yl)azetidin-3-yl)acetonitrile adipate (34.0 g, theoretical 39.7 g, 85.6% over two steps) as a white to off-white solid. 1HNMR(400MHz,DMSO-d6)δ12.16(s,1H),12.05(brs,2H),8.85(s,1H),8.72(s,1H),8.69(d,J=4.7Hz,1H),8.45(s,1H),7.93(t, J=4.7Hz,1H),7.63(dd,J=3.6,2.3Hz,1H),7.09(dd,J=3.6,1.7Hz,1H),δ4.11(dt,J=11.0,4.4Hz,1H),3.77(d,J=7.8Hz,2H),3. 60(t,J=7.8Hz,2H),3.58(s,2H),3.44(dt,J=14.4,4.6Hz,1H),3.28(t,J=10.4Hz,1H),3.09(ddd,J=13.2,9.6,3.2Hz,1H),2.58 (tt,J=8.6,3.5Hz,1H),2.28-2.17(m,4H),1.83-1.74(m,1H),1.67(d,J=11.0Hz,1H),1.59-1.46(m,4H),1.37-1.21(m,2H)ppm; 13 C NMR(101MHz,DMSO-d6)δ174.38,160.29,(153.52,150.87),152.20,150.94,149.63,(146.3 0,146.25),139.48,(134.79,134.62),(135.08,134.97,134.74,134.62,134.38,134.28,13 4.04,133.93),129.21,127.62,126.84,122.05,(124.75,122.02,119.29,116.54),117.39 ,113.01,99.99,61.47,60.50,57.06,44.24,33.42,30.70,28.63,27.89,27.20,24.07ppm;C 32 H 33 F4N9O5 (molecular weight 699.66; free radical についてC 26 H 23 F4N9O, molecular weight, 553.51), LCMS (EI) m / e 554.0 (M + +H).

[0602] Various modifications of the present invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are intended to fall within the scope of the appended claims. Each reference, including all patents, patent applications, and publications cited in this application, is hereby incorporated by reference in its entirety. The present application also includes the following aspects. [Aspect 1] 1. A process for preparing itacitinib, or a salt thereof, comprising: Compounds of Formula 3: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, or a salt thereof, and (ii) a compound of formula 2b: [ka] (In the formula, X - is a counter anion). [Aspect 2] 1. A process for preparing itacitinib, or a salt thereof, comprising: Compounds of Formula 3: [ka] or a salt thereof, by combining (i) a salt of formula 2a and (ii) a compound of formula 2b: [ka] (In the formula, X - is a counter anion). [Aspect 3] 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 50: [ka] or a salt thereof, by reacting (i) a salt of formula 2a, or a salt thereof, and (ii) a compound of formula 2b: [ka] to form a compound of formula 51: [ka] (In the formula, X - is the counter anion, and P 50 is an amino protecting group. [Aspect 4] 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 50:

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Claims

1. 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 3: 【Chemistry 1】 or a salt thereof, by reacting (i) a salt of formula 2a, or a salt thereof, and (ii) a compound of formula 2b: 【Chemistry 2】 (In the formula, X － is a counter anion.

2. 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 3: 【Transformation 3】 or a salt thereof, by combining (i) a salt of formula 2a and (ii) a compound of formula 2b: 【Chemistry 4】 (In the formula, X － is a counter anion.

3. 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 50: 【Transformation 5】 or a salt thereof, by reacting (i) a salt of formula 2a, or a salt thereof, and (ii) a compound of formula 2b: 【Transformation 6】 to form a compound of formula 51: 【Transformation 7】 (In the formula, X － is the counter anion, and P ５０ is R 50 —O—C(O)—, where R 50 is C 1-6 alkyl.

4. 1. A process for preparing itacitinib, or a salt thereof, comprising: Compound of Formula 50: 【Transformation 8】 or a salt thereof, by combining (i) a salt of formula 2a and (ii) a compound of formula 2b: 【Chemistry 9】 to form a compound of formula 51: 【Chemistry 10】 (In the formula, X － is the counter anion, and P ５０ is R 50 —O—C(O)—, where R 50 is C 1-6 alkyl.

5. R ５０ The process of claim 3 or 4, wherein is methyl, ethyl, propyl, isopropyl, butyl or t-butyl.

6. P ５０ The process of claim 3 or 4, wherein is t-butyl-O—C(O)—.

7. The compound of formula 51 is deprotected to give a compound of formula 52: 【Chemistry 11】 7. The process of any one of claims 3 to 6, further comprising forming methylaminobenzoate or a salt thereof.

8. 8. The process of claim 7, wherein said deprotecting said compound of formula 51 comprises treating said compound of formula 51 with HCl.

9. 9. The process of claim 7 or 8, wherein the compound of formula 52, or a salt thereof, is the dihydrochloride salt of the compound of formula 52.

10. The compound of formula 52, or a salt thereof, is reacted with a compound of formula 53: 【Chemistry 12】 in the presence of sodium triacetoxyborohydride and a base to form itacitinib.

11. 11. The process of claim 10, wherein the base is triethylamine.

12. X － But Cl － , B.F. ４ － , P.F. ６ － , AsF ６ － , SbF ６ － , and ClO ４ － The process according to any one of claims 1 to 11, wherein the process is selected from

13. X － But Cl － The process according to any one of claims 1 to 11, wherein

14. The process of any one of claims 1 to 13, wherein the reagent is a salt of formula 2a.

15. The process of any one of claims 1 to 13, wherein the reagent is a compound of formula 2b.

16. wherein the salt of formula 2a or the compound of formula 2b is Compound of Formula 1a: 【Chemistry 13】 16. The process of any one of claims 1 to 15, wherein the compound is prepared by a process comprising reacting 2-(2-methyl-2-propanol)-4-hydroxybenzoic acid, or a salt thereof, with Vilsmeier reagent formed from dimethylformamide.

17. 17. The process of claim 16, wherein the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent.

18. 18. The process of claim 17, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, and triphosgene.

19. 18. The process of claim 17, wherein the chlorinating agent is oxalyl chloride.

20. The process of any one of claims 16 to 19, wherein the product of the reaction with the Vilsmeier reagent has formula 2d: 【Chemistry 14】

21. Formula 2d: 【Chemistry 15】 21. The process of claim 20, further comprising reacting the salt of formula 2c with a base to form a salt of formula 2c. 【Chemistry 16】

22. The process of any one of claims 16 to 19, wherein the reaction with the Vilsmeier reagent produces a salt of formula 2c: 【Chemistry 17】

23. The above formula 2c: [Chemistry 18] The salt of formula M ＋ X － with a salt of formula 2a to form a salt of formula 2b, M ＋ is the countercation, X － is Cl － 23. The process of claim 21 or 22, wherein the counter anion is other than

24. 24. The process of any one of claims 21 to 23, wherein the compound of formula 2b is prepared by a process comprising reacting the salt of formula 2a or the salt of formula 2c with a base to form the compound of formula 2b.

25. The compound of formula 1a, or a salt thereof, Formula 1aP: 【Chemistry 19】 (In the formula, P １ The process of any one of claims 16 to 24, wherein: is prepared by a process comprising deprotecting a compound of formula (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

26. R １ 26. The process of claim 25, wherein is methyl, ethyl, propyl, isopropyl, butyl, or t-butyl.

27. The compound of formula 1aP is Formula 2P: 【Chemistry 20】 with MeMgBr in the presence of iron(III) acetylacetonate, In the formula, P １ 27. The process of any one of claims 25 or 26, wherein is (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

28. The compound of formula 2P is Formula 12a: 【Chemistry 21】 to form said compound of formula 2P.

29. The protection may be achieved by reacting the compound of formula 12a with an alkali metal hydride and P １ 29. The process of claim 28, comprising reacting with -Y, wherein Y is halo.

30. P １ -Y is (R １ ) ３ Si － Y, where Y is halo, and R １ is C １～６ 30. The process of claim 29, wherein the alkyl is alkyl.

31. The compound of formula 12a is Formula 11a: 【Chemistry 22】 or a salt thereof with a strong acid.

32. The compound of formula 11a, or a salt thereof, Formula 10a: 【Chemistry 23】 32. The process of claim 31 , wherein the compound is prepared by a process comprising reacting a compound of formula (I) with (methoxymethyl)triphenylphosphonium chloride and a base.

33. The compound of formula 10a, or a salt thereof, Formula 9a: 【Chemistry 24】 33. The process of claim 32, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with ammonia.

34. The compound of formula 9a is Formula 8a: 【Chemistry 25】 34. The process of claim 33, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with Vilsmeier reagent formed from dimethylformamide.

35. 35. The process of claim 34, wherein the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent.

36. The compound of formula 12a is Formula 15a: 【Chemistry 26】 31. The process of any one of claims 28 to 30, wherein the compound is prepared by a process comprising reacting a compound of the formula: with a chlorinating agent.

37. The compound of formula 15a is Formula 14a: 【Chemistry 27】 with formamidine acetate and an alkali metal hydroxide to produce a compound of formula 14aa; 【Chemistry 28】 37. The process of claim 36, prepared by a process comprising reacting the compound of formula 14aa with a strong acid.

38. The compound of formula 14a is Formula 13a: 【Chemistry 29】 38. The process of claim 37, wherein the compound is prepared by a process comprising reacting the compound of formula (I) with bromoacetaldehyde diethyl acetal and sodium tert-amyloxide.

39. The compound of formula 1a or a salt thereof Formula 23P: 【Transformation 30】 (In the formula, P ２ A process according to any one of claims 16 to 24, wherein is prepared by a process comprising reducing a compound of the formula (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

40. 40. The process of claim 39, wherein said reduction of said compound of formula 23P is accomplished by a process comprising reacting said compound of formula 23P with hydrogen gas in the presence of a catalyst.

41. The compound of formula 23P is Formula 22P: 【Chemistry 31】 with MeMgBr in the presence of iron(III) acetylacetonate, In the formula, P ２ The process of claim 39 or 40, wherein is (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

42. The compound of formula 22P is Formula 22a: 【Chemistry 32】 to form said compound of formula 22P.

43. The protection may be achieved by reacting the compound of formula 22a with an alkali metal hydride and P ２ 43. The process of claim 42, comprising reacting with -Y, wherein Y is halo.

44. P ２ (R １ ) ３ Si, where R １ is C １～６ 42. The process of claim 41 , wherein the alkyl is alkyl.

45. The compound of formula 1a or a salt thereof Formula 18a: 【Transformation 33】 or a salt thereof with an acid to form said compound of formula 1a or a salt thereof.

46. The compound of formula 18a or a salt thereof is Formula 17a: 【Transformation 34】 or a salt thereof with formamidine acetate and triethyl orthoformate to form said compound of formula 18a or a salt thereof.

47. The compound of formula 17a or a salt thereof is Formula 20a: 【Chemistry 35】 or a salt thereof with a compound of formula 21a: 【Transformation 36】 with a compound of formula 17a to form said compound of formula 17a or a salt thereof.

48. The compound of formula 20a or a salt thereof is Formula 19a: 【Chemistry 37】 with bromo-1,1-dimethoxyethane and a base to form said compound of formula 20a or a salt thereof.

49. 49. The process of claim 48, wherein the base is an alkali metal carbonate.

50. The compound of formula 17a or a salt thereof is Formula 16a: 【Transformation 38】 47. The process of claim 46, wherein the compound is prepared by a process comprising reacting a compound of formula 17a with ethyl acetate and a base to form said compound of formula 17a or a salt thereof.

51. 51. The process of claim 50, wherein the base is an alkali metal alkoxide.

52. wherein the salt of formula 2a or the compound of formula 2b is Compound of formula 5a: 【Chemistry 39】 16. The process of any one of claims 2 and 4 to 15, wherein the compound is prepared by a process comprising reacting 2-(2-methyl-2-propanol), or a salt thereof, with Vilsmeier reagent formed from dimethylformamide.

53. 53. The process of claim 52, wherein the Vilsmeier reagent is prepared by a process comprising reacting dimethylformamide with a chlorinating agent.

54. 54. The process of claim 53, wherein the chlorinating agent is selected from oxalyl chloride, phosphorus oxychloride, and triphosgene.

55. 54. The process of claim 53, wherein the chlorinating agent is oxalyl chloride.

56. 56. The process of any one of claims 52 to 55, wherein the product of the reaction with the Vilsmeier reagent has formula 2d: 【Chemistry 40】

57. Formula 2d: 【Chemistry 41】 57. The process of claim 56, further comprising reacting a salt of formula 2c with a base to form a salt of formula 2c 【Chemistry 42】

58. 56. The process of any one of claims 52 to 55, wherein the reaction with the Vilsmeier reagent produces a compound of formula 2c 【Chemistry 43】

59. Formula 2c: 【Chemistry 44】 The salt of formula M ＋ X － with a salt of formula 2a to form a salt of formula 2b, M ＋ is the countercation, X － is Cl － 59. The process of claim 57 or 58, wherein the counter anion is other than

60. 60. The process of any one of claims 57 to 59, wherein the compound of formula 2b is prepared by a process comprising reacting the salt of formula 2a or the salt of formula 2c with a base to form the compound of formula 2b.

61. The compound of formula 5a, or a salt thereof, Formula 27a: 【Chemistry 45】 61. The process of any one of claims 52 to 60, wherein the compound is prepared by a process comprising hydrolyzing the compound of formula (I) in water in the presence of a base.

62. 62. The process of claim 61, wherein the base present for the hydrolysis of the compound of formula 27a is sodium hydroxide and the compound of formula 5a, or salt thereof, is the sodium salt of formula 5a.

63. 63. The process of claim 62, further comprising reacting the sodium salt of the compound of formula 5a with a strong acid.

64. The compound of formula 27a is Formula 26P: 【Chemistry 46】 (In the formula, P １ is p-toluenesulfonyl) with a strong acid.

65. The compound of formula 26P is Formula 25P: 【Chemistry 47】 (In the formula, P １ is p-toluenesulfonyl) with an alkali metal alkoxide to form said compound of formula 26P.

66. The compound of formula 25P is Formula 2P: 【Chemistry 48】 (In the formula, P １ is p-toluenesulfonyl) with diethyl malonate and a base.

67. 67. The process of any one of claims 1 to 66, wherein the itacitinib, or salt thereof, is itacitinib adipate.

68. 68. The process of claim 67, wherein said itacitinib adipate is prepared by a process comprising reacting said itacitinib with at least one equivalent of adipic acid.

69. The compound of formula 3, or a salt thereof, Formula A1: 【Chemistry 49】 69. The process of any one of claims 1, 2 and 5-68, wherein the compound is formed by a process comprising reacting a compound of the formula: with hydrazine.

70. The compound of formula 50, or a salt thereof, Formula 54: [Transformation 50] 5. The process of claim 3 or 4, wherein the compound is prepared by a process comprising reacting a compound of formula 50 with hydrazine to form said compound of formula 50, or a salt thereof.

71. 1. A process for preparing itacitinib, or a salt thereof, comprising: 【Chemistry 51】 with a salt of formula 3: 【Chemistry 52】 or a salt thereof to form said itacitinib or a salt thereof.

72. 1. A process for preparing itacitinib, or a salt thereof, comprising: 【Chemistry 53】 with a salt of formula 50: 【Chemistry 54】 or a salt thereof to form a compound of formula 51: 【Transformation 55】 (In the formula, P ５０ is R 50 —O—C(O)—, where R 50 is C 1-6 alkyl, or a salt thereof; The compound of formula 51 is deprotected to give a compound of formula 52: 【Transformation 56】 or a salt thereof, and The compound of formula 52, or a salt thereof, is reacted with a compound of formula 53: 【Chemistry 57】 in the presence of sodium triacetoxyborohydride and a base to form itacitinib, or a salt thereof.

73. 73. The process of claim 71 or 72, further comprising reacting the itacitinib with at least one equivalent of adipic acid.

74. 74. The process of any one of claims 71 to 73, wherein the itacitinib, or salt thereof, is itacitinib adipate.

75. The salt of formula 2c is a salt of formula 2d: 【Transformation 58】 75. The process of any one of claims 71 to 74, wherein the compound is prepared by a process comprising reacting a salt of formula 2c with a base to form the salt of formula 2c.

76. wherein the salt of formula 2d is Formula 2P: 【Chemistry 59】 with MeMgBr in the presence of iron(III) acetylacetonate to form a compound of formula 1aP; 【Transformation 60】 The compound of formula 1aP is deprotected to give a compound of formula 1a: 【Chemistry 61】 or a salt thereof, and reacting the compound of formula 1a, or a salt thereof, with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form the salt of formula 2d; In the formula, P １ 76. The process of claim 75, wherein is (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

77. wherein the salt of formula 2d is Formula 22P: 【Transformation 62】 with MeMgBr in the presence of iron(III) acetylacetonate to form a compound of formula 23P; 【Transformation 63】 The compound of formula 23P is reduced to give a compound of formula 1a: 【Chemistry 64】 or a salt thereof, and reacting the compound of formula 1a, or a salt thereof, with a Vilsmeier reagent formed from dimethylformamide and a chlorinating agent to form the salt of formula 2d; In the formula, P ２ 76. The process of claim 75, wherein is (R 1 ) 3 Si, where R 1 is C 1-6 alkyl.

78. The following formula: 【Transformation 65】 or a salt thereof, wherein P ５０ is R 50 —O—C(O)—, where R 50 is C 1-6 alkyl.

79. The following formula: 【Chemical Formula 66】 79. The compound of claim 78, or a salt thereof, having the formula:

80. The following formula: 【Transformation 67】 A compound of formula 3 having the formula:

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