Use of a traditional Chinese medicine composition in preparing a medicament for treating neurogenic pulmonary edema

Abstract

Provided is a traditional Chinese medicine composition for treating neurogenic pulmonary edema. The traditional Chinese medicine composition is mainly prepared with Qiang Huo, Angelica Root, Poria Cocos, Fangfeng, Scutellaria Baicalensis, Cnidium Root, Platycodon Root, Bupleurum Root, Qianhu, Citrus Fruit Root, Licorice Root, and Licorice Root. The traditional Chinese medicine composition can improve the lung index and lung wet weight / dry weight of rats with neurogenic pulmonary edema, and has a therapeutic effect on neurogenic pulmonary edema.

Description

[Technical Field]

[0001] The present invention belongs to the field of medicine and relates to a new use of a traditional Chinese medicine composition, particularly to the use of a traditional Chinese medicine composition comprising Scutellaria Baicalensis, Fangfeng Root, Qianhuo Root, Angelica Root, Bupleurum Root, Qianhuo Root, Cnidium Rhizome, Zhihua Root, Poria Cocos Root, Platycodon Root, and Licorice Root in the preparation of a medicament for treating neurogenic pulmonary edema. [Background technology]

[0002] Pulmonary edema is caused by fluid accumulation in the pulmonary interstitium or alveoli, resulting in an increase in extravascular pulmonary fluid volume and / or increased capillary pressure. The main clinical symptoms of acute pulmonary edema include dyspnea, cyanosis, cough, colorless or pink bubbles, and diffuse pulmonary rales, and in severe cases, it can lead to respiratory failure and death. Pulmonary edema is a common and serious disease with a high incidence and poor prognosis.

[0003] Pulmonary edema can be divided into cardiogenic and non-cardiogenic pulmonary edema. Cardiogenic pulmonary edema occurs when a large amount of blood accumulates in the pulmonary circulation due to a sudden increase in blood return volume or right ventricular output or a sudden decrease in left ventricular output caused by heart disease, causing a sudden increase in pulmonary capillary venous pressure. When the pressure in the pulmonary capillaries exceeds the colloid oncotic pressure, the hemodynamics within the capillaries changes, causing congestion in the pulmonary circulation, increasing the permeability of the pulmonary capillary walls and allowing fluid to filter through the capillary walls, resulting in pulmonary edema.

[0004] Although there are many causes of noncardiogenic pulmonary edema, its etiology is primarily due to elevated alveolar-capillary pressure, increased alveolar-capillary permeability, decreased plasma oncotic pressure, or other factors such as impaired pulmonary lymphatic reflux or increased negative pressure within tissue compartments. Neurogenic pulmonary edema refers to a clinical syndrome of acute pulmonary edema caused by acute interstitial and alveolar fluid leakage due to brain injury or central nervous system disease, despite the absence of primary cardiac or pulmonary disease.

[0005] In clinical practice, pulmonary edema is treated with antibiotics, corticosteroids, diuretics, vasodilators, etc. depending on the cause of the edema. However, these drugs have side effects to varying degrees. For example, vasodilators can cause a drop in blood pressure, and diuretics can cause electrolyte imbalance.

[0006] The term "pulmonary edema" does not exist in ancient medical texts, nor is it recorded in ancient Chinese medical texts, but the symptoms of pulmonary edema are similar to those described in Chinese medicine as "zhiyin," "panting," "lung swelling," "watery qi," and "upper respiratory cough." According to the main clinical symptoms of pulmonary edema and ancient understanding and modern clinical practice, the causes of pulmonary edema are threefold: external evil spirits attacking the lungs, heart disease and lung disease, and weakening of physical strength due to chronic illness.

[0007] Jingfang Granules are a combination of Jingjiang, Fangfeng, Qianhuo, Angelica Root, Bupleurum Root, Quercus Root, Cnidium Rhizome, Zizania Root, Poria Cocos, Platycodon Root, and Licorice Root. They have a diaphoretic effect, relieve superficial symptoms, clear wind and phlegm, purify the lungs, and relieve coughs. They are known to be very effective in treating a range of symptoms caused by exogenous wind and cold, and are safe with few side effects. Summary of the Invention

[0008] The present invention provides a novel herbal composition for treating neurogenic pulmonary edema, which is prepared using Szechuan Root, Fangfeng Root, Qiang Huo Root, Angelica Root, Bupleurum Root, Oriental Landraceae, Bupleurum Root, Cnidium Root, Zi Hua Root, Poria Root, Platycodon Root, and Licorice Root. The present invention is a further development of the use of the existing product Jingfang Granules. Based on feedback from clinical use, the inventors have researched and developed new uses for Jingfang Granules and their formulations. Experimental studies have shown that Jingfang Granules can significantly reduce the lung index and lung wet weight / dry weight in rats with oleic acid-induced neurogenic pulmonary edema, improve the pathological state of rat lung tissue, and reduce the degree of pulmonary tissue edema. Jingfang Granules prepared with the herbal composition of the present invention also significantly reduced the lung wet weight / dry weight in mice with neurogenic pulmonary edema induced by spinal cord injury. ,lung It can reduce the degree of tissue edema and increase the number of drugs for the clinical treatment of neurogenic pulmonary edema.

[0009] Jingjiao: Jingjiao is warm in nature and has the effect of warming stimulating heat and relieving superficial wind. It can be widely used for patients with wind-chill and wind-heat, as well as those without obvious chills or fever. Jingjiao also has the role of curing rashes and resolving sores, and has a certain therapeutic effect on diseases such as sores, rubella, and measles, and can also relieve itching. Furthermore, when Jingjiao is fried over charcoal, its properties change from pungent and warm to bitter and mild, and it has a certain hemostatic effect, which can improve symptoms such as bloody stools, postpartum bleeding, and uterine bleeding.

[0010] Fangfeng: Fangfeng has pungent and warming properties, and has the effect of dispelling wind and relieving superficial symptoms, and has a certain effect on headaches, sweating, fever, etc. caused by wind-chill colds. Fangfeng also has the effect of dispelling dampness and relieving pain, and can relieve rheumatic joint pain, arthralgia, and other symptoms. Fangfeng also has an antispasmodic effect, and can improve the symptoms of cramps in the hands and feet. Modern pharmacological research has shown that Fangfeng has sedative, analgesic, and anticonvulsant effects, and is often used as an adjunct treatment for tetanus.

[0011] Qiang Huo: In traditional Chinese medicine, Qiang Huo is believed to have pungent, bitter, and warming properties, affecting the bladder and kidneys, relieving surface symptoms, clearing cold, wind, and dampness, and relieving pain. First, Qiang Huo is pungent and has a dissipating and stimulating effect, relieving surface symptoms. Furthermore, its warming properties allow it to treat wind-chill symptoms of colds. Second, Qiang Huo has warming and drying properties, clearing dampness. In addition to relieving surface symptoms, it also clears wind and overcomes dampness, allowing it to treat wind-chill, dampness, and numbness of the upper body. Qiang Huo also has an analgesic effect and can be used externally or internally. Modern pharmacological research has shown that the volatile oil of Qiang Huo has significant antipyretic and analgesic effects and inhibits skin fungi.

[0012] Angelica Root Extract: Angelica Root Extract harmonizes the meridians, improves the flow of qi and blood, and has the effects of dispelling wind and dampness, alleviating symptoms such as joint pain, decreased joint flexion and extension, and paralysis of the hands and feet caused by wind-cold dampness. Angelica Root Extract can also be used to relieve symptoms such as toothache caused by wind and fire and headache caused by wind. It is commonly used clinically to treat diseases such as rheumatoid arthritis, rheumatoid arthritis, sciatica, cervical spondylosis, and frozen shoulder.

[0013] Bupleurum Root: Bupleurum Root acts on the liver, releasing liver qi stagnation and improving liver qi circulation, and has a certain regulating effect on symptoms such as chest and hypochondrium pain, menstrual irregularities, mental depression, and pain due to breast distension. Bupleurum Root has a slight cooling property and antipyretic effect, and is effective in relieving fever caused by diseases such as acute bronchitis, acute tracheitis, and viral pneumonia. Bupleurum Root also has the effect of strengthening yang and can improve symptoms such as qi deficiency and organ prolapse. Pharmacological studies have shown that Bupleurum Root has sedative and analgesic effects, and can protect the liver and reduce liver damage.

[0014] Qianhu: Qianhu is bitter, pungent, and slightly cold. It acts on the lungs, treating symptoms such as coughs with yellow phlegm, excessive or sticky phlegm, fever, dry mouth, and shortness of breath, which are caused by the accumulation of phlegm and heat in the lungs and the impairment of the lung's ability to dissipate and lower blood. It also helps to eliminate fire within the lungs, ease the upward movement of lung qi, and reduce fire, making it useful in treating infectious diseases such as bronchitis and pneumonia. Qianhu also has the effect of dispersing wind and clearing heat, making it suitable for patients with exogenous wind-heat, dispersing wind-heat, and relieving symptoms such as headaches, irritability, and insomnia.

[0015] Cnidium officinale: Cnidium officinale is a traditional Chinese medicine commonly used in clinical practice. It grows primarily in mild climates and has mild properties, acting on the liver, gallbladder, and pericardium. It promotes blood circulation, clears congestion, dispels wind, and relieves pain in patients with chest and flank pain, numbness caused by wind and dampness, irregular menstruation, and pain from falls, alleviating pain and regularizing menstruation. Furthermore, the volatile oil components contained in Cnidium officinale have a certain inhibitory effect on brain activity, preventing excessive excitement in the body and providing a calming effect.

[0016] Perilla husk: In traditional Chinese medicine, perilla husk, with its bitter taste, is believed to act on the spleen and stomach, improving qi flow, normalizing qi circulation and restoring function, relieving stagnation, and alleviating abdominal distension. Perilla husk is the dried unripe fruit of an orange. Its properties and meridian pathways are similar to those of perilla fruit, but its effects are milder. Its effects on improving qi flow, normalizing qi circulation and restoring function, and reducing bloating make it suitable for treating qi congestion, chest and hypochondria, bloating and pain, indigestion, flatulence, and sagging. Perilla husk is widely used in dietary therapy, primarily to strengthen the spleen and digestion, and to relieve symptoms of bloating and discomfort. Recent pharmacological studies have shown that perilla husk has the effect of regulating gastrointestinal motility and stimulating intestinal smooth muscle.

[0017] Poria cocoon: Poria cocoon has a gentle effect on the spleen. When steeped in water and drunk, it strengthens the spleen and has a certain regulating effect on spleen deficiency, loss of appetite, loose stools, and diarrhea. Poria cocoon also acts on the heart and has a calming effect, relieving symptoms such as panic, insomnia, and anxiety, and helping to improve sleep quality. Furthermore, when steeped in water and drunk, it has a diuretic and moistening effect, which can relieve symptoms such as dropsy, oliguria, and difficulty urinating.

[0018] Platycodon grandiflorum: Platycodon grandiflorum has a bitter, pungent, and soothing quality and acts on the lungs. Platycodon grandiflorum is usually used to treat symptoms such as cough with excessive phlegm, chest tightness, sore throat, hoarseness, lung abscess, and vomiting of pus. Platycodon grandiflorum water has the effect of lowering blood lipids and blood sugar levels.

[0019] Licorice: Licorice has a sweet and cool taste and acts on the heart, lungs, spleen, and stomach. It has the effect of replenishing qi and invigorating the pulse. After honey roasting, its ability to replenish qi is enhanced. It can be used to treat cardiac energy deficiency, palpitations, and slow pulse. It can clear heat and detoxify. Licorice has a sweet and cool taste and can clear heat and detoxify. It can be used to help treat sore throat and epilepsy. Licorice has a gentle nature and mild medicinal properties, and can clear phlegm, stop coughs, replenish qi, and moisten the lungs. It can be used to treat wind-cold cough, wind-heat cough, cold phlegm cough, wet phlegm cough, and dry cough. When licorice acts on the upper abdomen, it strengthens the spleen and stomach, soothing and relieving pain. It can also be used to treat spleen and stomach qi deficiency, fatigue, loss of appetite, loose stools, abdominal distension, and acute pain in the hands and feet.

[0020] The present invention provides a specific composition of the above-mentioned herbal medicine composition, which mainly comprises:

[0021] It is prepared using 5 to 30 parts by weight of thornwort and 5 to 30 parts by weight of windbreaker as raw materials.

[0022] The herbal medicine composition mainly comprises:

[0023] It is prepared using 10-20 parts by weight of thornwort and 15-25 parts by weight of baofu as raw materials.

[0024] The herbal medicine composition mainly comprises:

[0025] 5~30 parts by weight of Jinghuan, 5~30 parts by weight of windbreak, 5~30 parts by weight of Qinghui

[0026] It is prepared using 5 to 30 parts by weight of Angelica dahurica, 3 to 25 parts by weight of Bupleurum Root, and 3 to 25 parts by weight of Oriental Husk.

[0027] The herbal medicine composition mainly comprises:

[0028] 5~30 parts by weight of Jinghuan, 5~30 parts by weight of windbreak, 5~30 parts by weight of Qinghui

[0029] Dokatsu 5-30 parts by weight, Saiku 3-25 parts by weight, Qianhu 3-25 parts by weight

[0030] 5 to 30 parts by weight of Chinese cabbage, 3 to 25 parts by weight of Mantis annua, 5 to 30 parts by weight of Bokum

[0031] It is prepared using 3 to 25 parts by weight of Platycodon grandiflorum as a raw material.

[0032] The herbal medicine composition mainly comprises:

[0033] 5~30 parts by weight of Jinghuan, 5~30 parts by weight of windbreak, 5~30 parts by weight of Qinghui

[0034] Dokatsu 5-30 parts by weight, Saiku 3-25 parts by weight, Qianhu 3-25 parts by weight

[0035] 5 to 30 parts by weight of Chinese cabbage, 3 to 25 parts by weight of Mantis annua, 5 to 30 parts by weight of Bokum

[0036] It is prepared using 3 to 25 parts by weight of Platycodon grandiflorum and 1 to 10 parts by weight of Licorice as raw materials.

[0037] The herbal medicine composition mainly comprises:

[0038] 10~20 parts by weight of Jinghu, 10~20 parts by weight of windbreak, 10~20 parts by weight of Qinghui

[0039] Dokatsu 10~20 parts by weight, Saiku 5~20 parts by weight, Qianhu 5~20 parts by weight

[0040] 10 to 20 parts by weight of Cucumber, 5 to 20 parts by weight of Mantis annua, 10 to 20 parts by weight

[0041] It is prepared using 5 to 20 parts by weight of Platycodon grandiflorum and 3 to 10 parts by weight of Licorice as raw materials.

[0042] The herbal medicine composition mainly comprises:

[0043] Jingku 12-18 parts by weight, Windproof 12-18 parts by weight, Qianghu 12-18 parts by weight

[0044] Dokatsu 12-18 parts by weight, Saiku 10-18 parts by weight, Qianhu 10-20 parts by weight

[0045] 10 to 20 parts by weight of Cucumber, 10 to 20 parts by weight of Mantis annua, 10 to 20 parts by weight

[0046] It is prepared using 10 to 20 parts by weight of Platycodon grandiflorum and 3 to 8 parts by weight of Licorice as raw materials.

[0047] The herbal medicine composition mainly comprises:

[0048] 15 parts by weight of Jinghu, 15 parts by weight of windbreak, 15 parts by weight of Qianghu

[0049] Dokatsu 15 parts by weight, Saiba 15 parts by weight, Qianhu 15 parts by weight

[0050] 15 parts by weight of Cucumber, 15 parts by weight of Mantis annua, 15 parts by weight of Bulong

[0051] It is prepared using 15 parts by weight of Platycodon grandiflorum and 5 parts by weight of Licorice as raw materials.

[0052] The herbal medicine composition mainly comprises:

[0053] 15 parts by weight of Jinghu, 15 parts by weight of windbreak, 15 parts by weight of Qianghu

[0054] Dokatsu 15 parts by weight, Saiba 15 parts by weight, Qianhu 10 parts by weight

[0055] 10 parts by weight of Cucumber, 10 parts by weight of Mantis annua, 10 parts by weight of Bulong

[0056] It is prepared using 10 parts by weight of Platycodon grandiflorum and 5 parts by weight of Licorice as raw materials.

[0057] The present invention provides a method for preparing the above-mentioned herbal composition, which mainly includes the steps of first distilling Jingjiang, Fangfeng, Qianghuo, Euglena Root, Quanhuo, Cnidium Root and Citrus Fruit Flour to extract volatile oils for use, preparing the distilled drug residue, distilled Cnidium Root and Citrus Fruit Flour aqueous solution for use, and then carrying out other operations.

[0058] Specifically, the preparation method mainly includes:

[0059] Step A is to distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract volatile oils for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use;

[0060] Step B: The distilled Cnidium officinalis and Cnidium husk aqueous solution obtained in Step A is prepared into a 10-40% ethanol solution for use.

[0061] Step C is to mix the Poria cocos, the distilled Cnidium officinalis and the residue of the husk obtained in step A, and extract it by infusing it with the ethanol solution obtained in step B, and prepare the infuse liquid for use.

[0062] Step D is to add water to the Bupleurum Root, Platycodon grandiflorum, Licorice Root, and the distilled Jingjiao, Fangfeng, Qianghuo, Angelica Root, and Qianhuo medicinal residue obtained in Step A and boil them, and then concentrate the decoction for use.

[0063] and step E, mixing and concentrating the infusion liquid obtained in step C and the decoction obtained in step D, and adding the volatile oil obtained in step A to obtain a Chinese herbal medicine composition.

[0064] Furthermore, the preparation method mainly comprises:

[0065] Step A is to distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract volatile oils for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use;

[0066] Step B: Prepare the distilled Cnidium officinalis and Cnidium officinalis husk aqueous solution obtained in Step A into a 15-30% ethanol solution and prepare it for use.

[0067] Step C: Mixing Poria cocos, Cnidium officinalis and the residue of the distilled Cnidium husk obtained in step A, and extracting it by percolation with the ethanol solution obtained in step B, and preparing the percolation liquid for use;

[0068] Step D is to add water to the Bupleurum Root, Platycodon grandiflorum, Licorice Root, and the distilled Jingjiao, Fangfeng, Qianghuo, Angelica Root, and Oriental Huang Root residue obtained in Step A and boil them, then concentrate the decoction to make a ointment, which is then ready for use.

[0069] and step E, mixing the infusion liquid obtained in step C and the ointment obtained in step D, concentrating the mixture to form a ointment, and adding the volatile oil obtained in step A to obtain a traditional Chinese medicine composition.

[0070] The fourth object of the present invention is to provide a herbal medicine preparation containing the above-mentioned herbal medicine composition, which is an oral preparation acceptable for clinical treatment;

[0071] The pharmaceutical compositions of the present invention can be prepared by conventional formulation techniques using conventional pharmaceutical auxiliary substances.

[0072] Typical pharmaceutical auxiliary substances are one or more selected from excipients, lubricants, binders, emulsifiers, disintegrants, stabilizers, and flavoring agents.

[0073] The excipient is one or more selected from lactose, sucrose, glucose, mannitol sugar, sorbitol sugar, starch, dextrin, crystalline cellulose, gum arabic, and dextran.

[0074] The lubricant is one or more selected from magnesium stearate, calcium stearate, talc powder, fine particle silica gel, boric acid, and sodium lauryl sulfate.

[0075] The binder is one or more selected from the group consisting of hydroxypropyl cellulose, hydroxypropyl methyl cellulose, polyvinylpyrrolidone, and polyethylene glycol.

[0076] The disintegrant is one or more selected from low-substituted hydroxypropyl cellulose, carboxymethyl cellulose, sodium carboxymethyl starch, and cross-linked polyvinylpyrrolidone.

[0077] The emulsifier is one or more selected from bentonite, magnesium hydroxide, aluminum hydroxide, and sodium lauryl sulfate.

[0078] The stabilizer is one or more selected from methylparaben, benzyl alcohol, phenethyl alcohol, phenol, sorbic acid, and dehydroacetic acid.

[0079] The flavoring agent is one or more selected from sucrose, flavoring agents, aspartame, and cyclodextrin.

[0080] Furthermore, the clinically acceptable oral formulation is one or more of pills, capsules, tablets, granules, combinations, or oral liquids;

[0081] Furthermore, the oral formulation is a granule or a combination formulation.

[0082] The present invention also provides a use of the above herbal composition in preparing a medicament for treating neurogenic pulmonary edema.

[0083] Neurogenic pulmonary edema (NPE) is a clinical syndrome characterized by acute pulmonary edema following central nervous system (CNS) injury. NPE is primarily seen in patients with CNS lesions such as spinal cord injury, intracranial hemorrhage, status epilepticus, meningitis, subarachnoid hemorrhage, and medullary lesions, and typically occurs within 24–72 h of the onset of the CNS lesion.

[0084] Neurogenic pulmonary edema differs from acute respiratory distress syndrome in that patients do not have cardiac or pulmonary dysfunction, but instead suffer from central nervous system damage, resulting in elevated pulmonary extravascular hydrostatic pressure and impaired oxygenation. The common feature of neurogenic pulmonary edema is acute and severe central nervous system damage, characterized by a sudden, rapid rise in intracranial pressure, which leads to brain tissue compression, ischemia, and other damage, resulting in extreme excitation of the sympathetic nervous system, which releases catecholamines, causing an abnormal rise in pulmonary extravascular hydrostatic pressure and pulmonary edema.

[0085] Furthermore, the neurogenic pulmonary edema is pulmonary edema caused by brain injury or central nervous system disease.

[0086] Neurogenic pulmonary edema is a syndrome in which damage to the central nervous system, such as the medulla oblongata or hypothalamus, causes increased extrapulmonary hydrostatic pressure and impaired oxygenation, resulting in a series of lung lesions. Clinically, it is classified into two subtypes: acute and chronic.

[0087] Because the clinical symptoms of neurogenic pulmonary edema are not specific and can easily be confused with other lung diseases, acute respiratory distress and progressive hypoxemia are the most important clinical symptoms of neurogenic pulmonary edema. In the early stages of neurogenic pulmonary edema, nonspecific symptoms such as shortness of breath, elevated blood pressure, and elevated pulse rate may be present. However, in the later stages of neurogenic pulmonary edema, typical symptoms include dyspnea, shortness of breath, tachycardia, cyanosis, pink frothy sputum, pulmonary crepitus, and rales. These symptoms resemble respiratory distress syndrome, and oxygen therapy is generally ineffective.

[0088] Furthermore, the neurogenic pulmonary edema is one or more types selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, intracerebral tumor, acute cerebral edema, epilepsy, and viral infection accompanied by central nervous system lesions.

[0089] Neurogenic pulmonary edema (PE) is pulmonary edema caused by brain lesions such as cerebrovascular disease, brain tumors, brain trauma, and grand mal epilepsy. It is a clinical syndrome characterized by acute pulmonary edema after central nervous system injury.

[0090] Head trauma refers to injuries that occur to the head and is a common type of injury, with injuries caused by falls and collisions being the most common, followed by contusions. Head trauma often occurs in disasters, wars, and traffic accidents. It is important for injuries throughout the body. Head trauma can be classified into three categories: soft tissue (scalp) injuries, skull injuries (fractures), and intracranial tissue (cerebral blood vessels and meninges) injuries.

[0091] The surgery refers to a neurosurgery.

[0092] The subarachnoid hemorrhage is a clinical syndrome caused by the rupture of a diseased blood vessel at the base or surface of the brain, causing blood to flow directly into the subarachnoid space, and is also called primary subarachnoid hemorrhage.

[0093] Cerebral embolism refers to the blockage of a cerebral artery by various emboli (e.g., cardiac mural thrombus, atherosclerotic plaque, fat, tumor cells, fibrocartilage, or air) in the bloodstream, which cannot be compensated for by collateral circulation and causes ischemic necrosis of the brain tissue in the area supplied by the artery, resulting in local neurological deficits.

[0094] The cerebral hemorrhage refers to bleeding caused by non-traumatic rupture of blood vessels in the cerebral parenchyma, and its cause is closely related to cerebrovascular diseases, namely hyperlipidemia, diabetes, hypertension, vascular aging, smoking, etc. Patients with cerebral hemorrhage often suddenly develop symptoms due to emotional excitement or strenuous exercise, and the early mortality rate is very high, and most survivors are left with sequelae such as motor disorders, cognitive disorders, speech disorders, and swallowing disorders, although to varying degrees.

[0095] Brainstem infarction, also known as brainstem embolism, is the most serious type of cerebral infarction, and occurs when arteriosclerosis, embolism, spasm, inflammation, etc. of the vertebral basilar artery and its branches cause stenosis or occlusion of the arteries, resulting in ischemia of the midbrain, pons, and medulla oblongata, resulting in corresponding neurological symptoms and signs.

[0096] The intracerebral tumor refers to a brain tumor including a primary brain tumor occurring in the brain parenchyma and a secondary brain tumor metastasized to the brain from another part of the body.

[0097] Epilepsy, commonly known in China as "sheep horn wind" or "sheep den wind," is a chronic disease in which neurons in the brain suddenly discharge abnormally, causing temporary brain dysfunction.

[0098] Furthermore, the neurogenic pulmonary edema is one or more types selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, intracerebral tumor, acute cerebral edema, and viral infection accompanied by central nervous system lesions.

[0099] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, intracerebral tumor, acute cerebral edema, and epilepsy.

[0100] Furthermore, the neurogenic pulmonary edema is one or more types selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, intracerebral tumor, and acute cerebral edema.

[0101] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, and intracerebral tumor.

[0102] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, and brainstem infarction.

[0103] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, and cerebral hemorrhage.

[0104] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, and cerebral embolism.

[0105] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma, surgery, and subarachnoid hemorrhage.

[0106] Furthermore, the neurogenic pulmonary edema is one or more selected from pulmonary edema caused by head trauma and surgery.

[0107] Furthermore, the neurogenic pulmonary edema is selected from pulmonary edema caused by head trauma.

[0108] Pharmacological experiments have shown that Jingfang Granules prepared with the herbal composition of the present invention can significantly reduce the lung index and lung wet / dry weight in rats with neurogenic pulmonary edema caused by oleic acid, improve the pathological condition of the rat lungs, and reduce the degree of edema in lung tissue. Pharmacological experiments have also shown that Jingfang Granules can significantly reduce the lung wet / dry weight in mice with neurogenic pulmonary edema induced by spinal cord injury. ,lung The herbal composition of the present invention can reduce the degree of tissue edema, and increase the number of drugs for the clinical treatment of neurogenic pulmonary edema. [Brief explanation of the drawings]

[0109] [Figure 1] 1 shows lung tissue images of rats in each group in the oleic acid-induced acute pulmonary edema model. [Figure 2] 1 shows lung tissue images of rats in each group in the oleic acid-induced neurogenic pulmonary edema model. DETAILED DESCRIPTION OF THE INVENTION

[0110] Example 1 Preparation of Granules

[0111] prescription

[0112] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0113] Preparation method

[0114] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0115] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 25% ethanol solution for use.

[0116] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0117] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0118] Step E: The infiltrate obtained in Step C and the plaster obtained in Step D are mixed, left to stand, and filtered to concentrate into a plaster with a relative density of 1.30 (80-85°C). Weight part 6g sucrose powder Weight part The volatile oil obtained in step A was added and mixed uniformly, granulated, and dried, and the volatile oil obtained in step A was added and mixed uniformly to obtain granules.

[0119] Example 2 Preparation of Granules

[0120] prescription

[0121] Jinghui 50g, Windproof 100g, Chikatsu 50g, Solitary Katsu 100g, Saihu 15g, Qianhu 100g, Senkyu 50g, Kikhu 100g, Bouling 50g, Bellflower 100g, Licorice 5g

[0122] The preparation method was the same as in Example 1.

[0123] Example 3 Preparation of Granules

[0124] prescription

[0125] Jingju 100g, Windproof 50g, Chikatsu 100g, Solitary Katsu 50g, Saihu 100g, Qianhu 15g, Senkyu 100g, Beetroot husk 125g, Boului 125g, Bellflower 15g, Licorice 40g

[0126] The preparation method was the same as in Example 1.

[0127] Example 4 Preparation of oral solutions

[0128] prescription

[0129] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0130] Preparation method

[0131] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0132] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 10% ethanol solution for use.

[0133] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0134] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0135] Step E: The infusion liquid obtained in Step C and the plaster obtained in Step D were mixed, left to stand and filtered to concentrate into a plaster, an appropriate amount of sucrose was added and mixed uniformly, the volatile oil obtained in Step A was added and mixed uniformly, and water was added until the total volume was 1000 ml to obtain an oral solution.

[0136] Example 5 Preparation of syrup

[0137] prescription

[0138] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0139] Preparation method

[0140] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0141] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 40% ethanol solution for use.

[0142] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0143] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0144] Step E: The impregnation liquid obtained in Step C and the plaster obtained in Step D were mixed, allowed to stand, and filtered to concentrate into a plaster. An appropriate amount of sucrose was added and mixed uniformly. 500 ml of the volatile oil and simple syrup obtained in Step A were added, mixed uniformly, allowed to stand, and filtered. Water was added until the total volume reached 1000 ml to obtain a syrup.

[0145] Example 6 Preparation of tablets

[0146] prescription

[0147] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0148] Preparation method

[0149] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0150] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 15% ethanol solution for use.

[0151] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0152] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0153] Step E: The impregnation liquid obtained in Step C and the ointment obtained in Step D are mixed, left to stand and filtered, concentrated into a ointment, an appropriate amount of sucrose is added, the mixture is uniformly mixed, granulated and dried, the volatile oil obtained in Step A is added, the mixture is uniformly mixed, granulated, an appropriate amount of auxiliary materials are added, the mixture is uniformly mixed, and tableted to obtain tablets.

[0154] Example 7 Preparation of Capsules

[0155] prescription

[0156] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0157] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0158] Step B: The distilled aqueous solution of Cnidium officinalis and Citrus husk obtained in Step A was prepared into a 30% ethanol solution for use.

[0159] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0160] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0161] Step E: The impregnation liquid obtained in Step C and the plaster obtained in Step D are mixed, left to stand and filtered, concentrated into a plaster, an appropriate amount of sucrose is added, the mixture is uniformly mixed, granulated and dried, the volatile oil obtained in Step A is added, the mixture is uniformly mixed, granulated and dried, crushed, and packed into capsules to obtain capsules.

[0162] Example 8 Preparation of pills

[0163] prescription

[0164] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0165] Preparation method

[0166] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0167] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 25% ethanol solution for use.

[0168] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0169] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0170] Step E: The impregnation liquid obtained in Step C and the plaster obtained in Step D are mixed, left to stand and filtered, concentrated into a plaster, an appropriate amount of sucrose is added, mixed uniformly, granulated and dried, the volatile oil obtained in Step A is added, mixed uniformly, dried and pulverized, sieved, 40-60g of refined honey and an appropriate amount of water pan pills are added, dried, and pills are obtained.

[0171] Example 9 Preparation of Plaster

[0172] prescription

[0173] Jingju 75g, Windproof 75g, Qianghu 75g, Dokatsu 75g, Saihu 75g, Qianhu 75g, Senkyu 75g, Mantis husk 75g, Bouling 75g, Bellflower 75g, Licorice 25g

[0174] Preparation method

[0175] Step A: Distill Jingjiang, Fangfeng, Qianghuo, Angelica Root, Quanhuo, Cnidium Root, and Citrus Fruit husk to extract the volatile oil for use, and prepare the distilled drug residue, distilled Cnidium Root, and Citrus Fruit husk aqueous solution for use.

[0176] Step B: The distilled aqueous solution of Cnidium officinalis and Cnidium husk obtained in Step A was prepared into a 25% ethanol solution for use.

[0177] Step C: Poria cocos, the distilled Cnidium officinalis obtained in Step A, and the residue of the husk of the husk were mixed, and the ethanol solution obtained in Step B was percolated to extract the mixture, and the percolated liquid was prepared for use.

[0178] Step D: Add water to the Bupleurum Root, Platycodon Grandiflorum, Licorice Root, and the distilled Jingjiang, Fangfeng, Qianghuo, Angelica Root, and Qianhuo Root medicinal residue obtained in Step A, and decoct them twice for 1.5 hours each. The two decoctions are combined, filtered, and concentrated into a paste for use.

[0179] Step E: The impregnation liquid obtained in Step C and the ointment obtained in Step D were mixed, left to stand and filtered to concentrate into a ointment, and the volatile oil obtained in Step A was added and mixed uniformly to obtain a ointment.

[0180] In order to verify the effectiveness of the herbal composition of the present invention in treating neurogenic pulmonary edema, the inventors conducted relevant pharmacodynamic test studies. It should be noted that the drugs selected in the following pharmacodynamic tests are drugs obtained using representative formulations and preparation methods of the present invention. The inventors also conducted pharmacodynamic experiments on drugs obtained using other formulations and preparation methods included in the present invention. The experimental results show that drugs obtained using other formulations and preparation methods have the same or similar effects, but due to space limitations, they cannot be listed comprehensively here. Furthermore, the following pharmacodynamic experiments only used several representative animal models to verify the effects of the present invention.

[0181] All of the following experimental studies were conducted after the safety of the drugs had been demonstrated through acute and long-term toxicity tests, and all doses administered in the experimental studies were within the safe dose range.

[0182] Oleic acid is a highly toxic fatty acid that, when injected intravenously, stimulates vasoconstriction, increases pulmonary arterial pressure, damages vascular endothelial cells, increases alveolar-capillary membrane permeability, induces pulmonary interstitial and alveolar edema, reduces lung compliance, decreases functional residual capacity, and forms alveolar diaphragms. Oleic acid is a mature and commonly used tool for modeling pulmonary edema.

[0183] mouse Perform a laminectomy Spinal cord injury causing mouseInducing pulmonary edema in mice is a typical method for modeling neurogenic pulmonary edema animal models, and this method is simple, easy to implement, and has a high success rate in modeling.

[0184] Example of efficacy 1: Effect of Keibo Granules on a rat model of acute pulmonary edema induced by oleic acid

[0185] 1 material

[0186] 1.1 Animals

[0187] Male Wista rats, 6-8 weeks old, weighing 200-220 g, were provided by Lunan Pharmaceutical Co., Ltd. and allowed to adapt for one week before the experiment.

[0188] 1.2 Drugs and reagents

[0189] 1.2.1 Drugs

[0190] Example 1 of the present invention Granules

[0191] oleic acid

[0192] 1.2.2 Rat Dosage

[0193] Example 1 Granules: 4 g / kg (low dose), 8 g / kg (medium dose), 16 g / kg (high dose).

[0194] 2 Experimental process

[0195] 2.1 Animal Grouping, Modeling, and Dosing

[0196] Sixty rats were randomly divided into a blank group, a model group, an Example 1 low-dose group, an Example 1 medium-dose group, and an Example 1 high-dose group (12 rats per group). The blank group and the model group were intragastrically administered 10ml / kg of purified water. The Example 1 low-dose group, Example 1 medium-dose group, and Example 1 high-dose group were intragastrically administered 4g / kg, 8g / kg, and 16g / kg of Jingbang Granules, respectively, at a volume of 10ml / kg, once daily for two consecutive days. Thirty minutes after the completion of the third day of administration, rats in the remaining groups, except for the blank group, were injected with 0.2ml / kg of oleic acid via their tail vein. Time was measured immediately after the third day. After 6 hours, the rats were anesthetized and sacrificed. The lungs were removed and the lung tissues were examined and weighed. The lung index and lung wet weight / dry weight were calculated using the formula: lung index = lung weight / body weight. The left lower lung was weighed to obtain the wet lung weight, and the left lower lung was baked at 70°C for 48 hours until it reached the specified weight, after which it was weighed and obtained the dry lung weight.

[0197] 2.2 Statistical processing

[0198] Statistical processing was performed using SPSS17.0 software, and the measurement data

[0199]

number

[0200] Comparisons between groups were performed using one-way analysis of variance, and comparisons between both groups were performed using the t-test. P<0.05 was considered to indicate a statistically significant difference.

[0201] 3. Results and Conclusions

[0202] Observing the overall shape of the lungs in each group, the lung tissue of rats in the blank group was normal, smooth, non-congestive, and pale pink, while the lung tissue of rats in the model group was significantly enlarged, showed congestion and bleeding spots, was dark red, and when the trachea was cut, pink bubbles overflowed. Compared with the model group, the lungs of rats in the low-, medium-, and high-dose groups of Example 1 showed significantly reduced congestion, with only a small amount of bubbles or no obvious bubbles in the trachea. The experimental results show that the Jingfang Granules of the present invention can effectively alleviate pulmonary edema and pathological changes in tissues, and have a clear therapeutic effect on pulmonary edema.

[0203] Compared with the blank group, the lung index and lung wet weight / dry weight were significantly increased in the model group (P<0.05). Compared with the model group, the lung index was significantly decreased in the Example 1 low dose group, Example 1 medium dose group, and Example 1 high dose group (P<0.05, P<0.01), and the lung wet weight / dry weight was significantly decreased in the Example 1 medium dose group and Example 1 high dose group (P<0.05). The results are shown in Table 1. The experimental results showed that Jingfang Granules could significantly improve the pulmonary edema symptoms in rats.

[0204] [Table 1]

[0205] Note: blank group *P<0.05, **P<0.01 compared with the model group; # P<0.05, ## P<0.01.

[0206] Example 2: Effect of Keibo Granules on a rat model of neurogenic pulmonary edema induced by oleic acid

[0207] 1.Material

[0208] 1.1 Animals

[0209] Male SD rats, 6-8 weeks old, weighing 200-220 g, were provided by Lunan Pharmaceutical Co., Ltd. and allowed to adapt for one week before the experiment.

[0210] 1.2 Drugs

[0211] Example 1 of the present invention: Granules

[0212] oleic acid

[0213] 1.2.2 Dosage for rats

[0214] Example 1 Granules: 4 g / kg (low dose), 8 g / kg (medium dose), 16 g / kg (high dose).

[0215] 2. Experimental process

[0216] 2.1 Animal Grouping, Modeling, and Dosing

[0217] Fifty rats were randomly divided into a blank group, a model group, an Example 1 low dose group, an Example 1 medium dose group, and an Example 1 high dose group, with one rat in each group. blank group The model group received intragastric administration of purified water, while the Example 1 low-dose group, Example 1 medium-dose group, and Example 1 high-dose group received intragastric administration of 4 g / kg, 8 g / kg, and 16 g / kg of Example 1 granules, respectively. The granules were diluted with purified water and administered in a volume of 10 ml / kg once daily for two consecutive days. 30 minutes after administration was completed on the third day, the animals were anesthetized and immobilized. The skin of the parietal bone was incised, and a No. 7 needle was inserted vertically about 6 mm into the center of the parietal bone. When blood was removed by aspiration, 0.2 ml / kg of oleic acid was slowly injected, and the timing began. After 10 minutes, the rats were sacrificed, and the lungs were removed. The lung tissue was observed and weighed, and the lung index and lung wet weight / dry weight were calculated using the formula: lung index = lung weight / body weight. The left lower lung lobe was weighed to obtain the wet lung weight, and the left lower lung lobe was baked at 70°C for 48 hours until it reached the specified weight, which was then weighed to obtain the dry lung weight, and the wet lung weight / dry lung weight ratio was calculated.

[0218] 2.2 Statistical processing

[0219] Statistical processing was performed using SPSS17.0 software, and the measurement data

[0220]

number

[0221] Comparisons between groups were performed using one-way analysis of variance, and comparisons between both groups were performed using the t-test. P<0.05 was considered to indicate a statistically significant difference.

[0222] 3. Results and Conclusions

[0223] Observation of the lung tissue of rats in each group showed that the lungs of rats in the blank group were full, smooth, and pink in color, while the lungs of rats in the model group were significantly enlarged, dark red in color, and overflowing with a large amount of foam after tracheotomy. Compared with the model group, the severity of lesions was significantly reduced in the low, medium, and high dose groups of Example 1. The experimental results show that the Jingfang Granules of the present invention can effectively alleviate pulmonary edema and pathological changes in tissues, and have a clear therapeutic effect on pulmonary edema.

[0224] Compared with the blank group, the lung index and lung wet weight / dry weight in the model group were significantly increased (P<0.01), indicating that the model was successfully created. Compared with the model group, the lung index and lung wet weight / dry weight in the medium-dose group of Example 1 Granules and the high-dose group of Example 1 Granules were significantly decreased (P<0.05, P<0.01), the results are shown in Table 2. The experimental results showed that Jingfang Granules could significantly improve neurogenic pulmonary edema in rats.

[0225] [Table 2]

[0226] Note: *P<0.05 compared with blank group, **P<0.01 compared with model group # P<0.05, ## P<0.01.

[0227] Examples of medicinal effects 3 Jingfang granules for neurogenic pulmonary edema due to spinal cord injury mouse Impact on the model

[0228] 1.Material

[0229] 1.1 Animals

[0230] Male ICR mice, 4-6 weeks old, weighing 23-25 ​​g, were provided by Lunan Pharmaceutical Co., Ltd. and were allowed to adapt for one week before the experiment.

[0231] 1.2 Drugs

[0232] Example 1 of the present invention: Granules

[0233] Dexamethasone

[0234] 2. Experimental Process

[0235] 2.1 Animal Grouping, Modeling, and Dosing

[0236] Forty mice were randomly divided into 8 groups: sham surgery group, model group, dexamethasone group, Example 1 granules group, and dexamethasone + Example 1 granules group. The sham surgery group underwent laminectomy but no spinal cord injury. The model group and each treatment group underwent laminectomy and spinal cord injury. 24 h after modeling, the sham surgery group and model group received an intramuscular injection of saline (10 ml / kg) and intragastrically administered purified water (10 ml / kg). The Example 1 granules group received intragastrically administered Example 1 granules (18 g / kg). The dexamethasone group received an intramuscular injection of dexamethasone injection (0.025 mg / kg). The dexamethasone + Example 1 granules group received intragastrically administered Example 1 granules (18 g / kg) and intramuscularly administered dexamethasone injection (0.025 mg / kg). The animals were administered the drug for two consecutive days. On the third day after modeling, the animals were anesthetized and sacrificed, and the lungs were removed and weighed for wet weight. After drying in a 60°C oven for 48 hours, the lungs were removed and weighed for dry weight. The wet / dry weight ratio was calculated.

[0237] 2.2 Statistical processing

[0238] Statistical processing was performed using SPSS17.0 software, and the measurement data

[0239]

number

[0240] Comparisons between groups were performed using one-way analysis of variance, and comparisons between both groups were performed using the t-test. P<0.05 was considered to indicate a statistically significant difference.

[0241] 3. Results and conclusion

[0242] Compared with the sham-operated group, the wet / dry lung weight ratio in the model group was significantly increased (P<0.001), indicating successful modeling. Compared with the model group, the wet / dry lung weight ratio in the Example 1 Granules group and the dexamethasone + Example 1 Granules group was significantly decreased (P<0.001), and the effect was superior to that of the dexamethasone group (P<0.01). The results are shown in Table 3. The experimental results showed that Jingfang Granules had a significant protective effect against neurogenic pulmonary edema caused by spinal cord injury, and its efficacy was far superior to that of dexamethasone.

[0243] [Table 3]

[0244] NOTE: Compared with the sham-operated group, *** P<0.001, compared with the model group; # P<0.05, ## P<0.01, ### P<0.001 compared with the dexamethasone group && P<0.01, &&& P<0.001.

Claims

1. 1. Use of a Chinese herbal medicine composition comprising Scutellaria Baicalensis, Fangfeng Root, Qianhuo Root, Angelica Root, Bupleurum Root, Cnidium Rhizome, Zhihua Root, Poria Cocos Root, Platycodon Root, and Licorice Root in the preparation of a medicament for preventing or treating neurogenic pulmonary edema, wherein the Chinese herbal medicine composition comprises: 5 to 30 parts by weight of Jingku, 5 to 30 parts by weight of windbreak, 5 to 30 parts by weight of Qianghu, Dokatsu 5-30 parts by weight, Saiku 3-25 parts by weight, Qianhu 3-25 parts by weight, 5 to 30 parts by weight of Cucumber, 3 to 25 parts by weight of Mantis annua, 5 to 30 parts by weight of Bokum, Bellflower 3-25 parts by weight, licorice 1-10 parts by weight Use of a herbal medicine composition, characterized in that it is prepared using the above as a raw material.

2. 2. The use according to claim 1, wherein the neurogenic pulmonary edema is pulmonary edema caused by brain injury or central nervous system disease.

3. The use according to claim 1, characterized in that the neurogenic pulmonary edema is one or more selected from the group consisting of pulmonary edema caused by head trauma, surgery, subarachnoid hemorrhage, cerebral embolism, cerebral hemorrhage, brainstem infarction, intracerebral tumor, acute cerebral edema, epilepsy, and viral infection accompanied by central nervous system lesions.

4. The herbal medicine composition comprises: The use described in claim 1, characterized in that it is prepared using 15 weight parts of Jiang Jiang, 15 weight parts of Fangfeng, 15 weight parts of Qianhuo, 15 weight parts of Angelica Root, 15 weight parts of Bupleurum Root, 15 weight parts of Quercus Husk, 15 weight parts of Cnidium Rhizome, 15 weight parts of Zizhou Root ... Poria Cocos, 15 weight parts of Platycodon Root, and 5 weight parts of Licorice.

5. The use according to any one of claims 1 to 4, characterized in that the herbal composition is prepared into tablets, capsules, pills, granules, or oral liquid by adding one or more pharmaceutically acceptable pharmaceutical auxiliary substances.

6. The use according to claim 5, characterized in that the herbal composition is prepared into granules by adding one or more pharmaceutically acceptable auxiliary substances.

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