Detection chip and detection system

Abstract

The detection chip (10) and detection system (60) include a sample supply port (110) and at least one detection branch structure (120), each of which includes a detection well (141) communicating with the sample supply port (110) and a reaction reagent (150) contained in the detection well (141), and the detection unit (140) is configured to enable optical inspection of the reaction reagent (150) in the detection well (141). The detection chip (10) contributes to automated detection and to the realization of a portable detection device.

Description

[Technical Field]

[0001] (CROSS-REFERENCE TO RELATED APPLICATIONS) This application claims priority from Chinese Patent Application No. 202010367824.8 filed on April 30, 2020, the entire contents of which are incorporated herein by reference.

[0002] SUMMARY OF THE DISCLOSURE Embodiments of the present disclosure relate to detection chips and detection systems. [Background technology]

[0003] Microfluidic chip technology integrates common operational units, such as sample preparation, reaction, separation, and detection, into a chip with micron-scale microchannels in fields such as biology, chemistry, and medicine, to automate the entire reaction and analysis process. The chip used in this process is called a microfluidic chip, also known as a lab-on-a-chip. Microfluidic chip technology has advantages such as low sample consumption, fast analysis speed, ease of use as a portable device, and suitability for real-time and on-site analysis, and is therefore widely used in many fields such as biology, chemistry, and medicine. Summary of the Invention

[0004] At least one embodiment of the present disclosure provides a detection chip including a sample supply port and at least one detection branching structure, each of the at least one detection branching structure including a detection well and a detection section including a reaction reagent, the detection well communicating with the sample supply port, the reaction reagent contained in the detection well, and the detection section configured to enable optical inspection of the reaction reagent in the detection well.

[0005] For example, in the detection chip provided in at least one embodiment of the present disclosure, each of the at least one detection branch structure further includes a flow guide groove, the flow guide groove having a first end and a second end, the first end of the flow guide groove communicating with the sample supply port, and the second end of the flow guide groove communicating with the detection well, thereby the detection well communicating with the sample supply port through the flow guide groove.

[0006] For example, the detection chip provided in at least one embodiment of the present disclosure further includes a first substrate having a first surface, wherein the sample supply port is a through-hole of the first substrate, and the flow guide groove and the detection well are formed on the first surface of the first substrate, and a second substrate stacked on the first surface of the first substrate and enabling the optical inspection at a position corresponding to the detection well.

[0007] For example, in the detection chip provided in at least one embodiment of the present disclosure, each of the at least one detection branch structure further includes a water-absorbing membrane, which is contained within the detection well and at least partially overlaps with the reaction reagent in a direction perpendicular to the first substrate.

[0008] For example, in the detection chip provided in at least one embodiment of the present disclosure, the water-absorbing film is provided on the side of the reaction reagent that is remote from the second substrate.

[0009] For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage capacity of the water-absorbent membrane is 10 μL to 50 μL.

[0010] For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection portion further includes a flow well located around the detection well, the flow well is connected to the detection well, and the height of at least a portion of the flow well is lower than the height of the detection well.

[0011] For example, in the detection chip provided in at least one embodiment of the present disclosure, the flow-directing well includes an inclined flow-directing wall, one end of which is connected to a side surface of the detection well.

[0012] For example, in the detection chip provided in at least one embodiment of the present disclosure, the longitudinal cross sections of the flow guide well and the detection well are generally stepped.

[0013] For example, in at least one embodiment of the present disclosure, in the sensing chip provided, at least a portion of the flow well surrounds the sensing well.

[0014] For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the detection well is 0.2 mm to 5 mm, the difference between the maximum height of the flow well and the height of the detection well is 0.1 mm to 1 mm, and the width of the flow well is 0.1 mm to 1 mm.

[0015] For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection unit further includes a liquid storage through-hole that penetrates the first substrate and communicates with the detection well.

[0016] For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage through-hole communicates with the center of the detection well.

[0017] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the liquid storage through-hole is 0.2 mm to 5 mm, and the ratio of the depth of the liquid storage through-hole to the height of the detection well is 0.5:1 to 10:1.

[0018] For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the flow guiding groove is 0.1 mm to 1.5 mm, and the width of the flow guiding groove is 0.1 mm to 2 mm.

[0019] For example, in the detection chip provided in at least one embodiment of the present disclosure, the ratio of the height of the flow guiding groove to the width of the flow guiding groove is 1:1 to 10:1.

[0020] For example, in the detection chip provided in at least one embodiment of the present disclosure, the inner wall of the flow guide channel is hydrophilic.

[0021] For example, in the detection chip provided in at least one embodiment of the present disclosure, the at least one detection branching structure includes a plurality of detection branching structures, and the plurality of detection branching structures are evenly arranged around the sample supply port.

[0022] For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample supply port includes a first body and a first convex portion protruding from the first body into the flow guide groove, and the first convex portion is connected to the flow guide groove.

[0023] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the first body is 1 mm to 10 mm.

[0024] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a reaction film and / or a bulk reaction reagent.

[0025] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction film is compressed in the thickness direction.

[0026] For example, in the detection chip provided in at least one embodiment of the present disclosure, the ratio of the thickness of the reaction film in a relaxed state to the height of the detection well is 1:1 to 1:0.5.

[0027] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction film is circular, or the reaction film is polygonal, and one corner of the polygonal shape is directly connected to the second end of the flow guide groove.

[0028] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction film includes a film body and a second convex portion protruding from the film body into the flow groove, at least a portion of the second convex portion is located within the flow groove, and the film body is circular, similar to the detection well.

[0029] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction film is diamond-shaped.

[0030] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection well is 3 mm to 15 mm, and the diameter of the reaction film is equal to or smaller than the diameter of the detection well.

[0031] For example, in the detection chip provided in at least one embodiment of the present disclosure, the second substrate has detection through-holes at positions corresponding to the detection wells.

[0032] For example, in the detection chip provided in at least one embodiment of the present disclosure, the second substrate is non-transparent, or the detection chip further includes a light-shielding layer, which is covered on the surface of the second substrate that is away from and / or close to the first substrate and exposes the detection through-hole.

[0033] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection through-hole is 2 mm to 10 mm.

[0034] For example, in the detection chip provided in at least one embodiment of the present disclosure, the material of the first substrate includes one or more of polymethyl methacrylate, polystyrene, and polycarbonate.

[0035] For example, in the detection chip provided in at least one embodiment of the present disclosure, the material of the second substrate includes one or more of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate.

[0036] For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate and the second substrate are joined together by bonding, welding, adhesion, or clinging.

[0037] For example, the detection chip provided by at least one embodiment of the present disclosure further includes an adhesive layer, which is located between the first substrate and the second substrate and is for bonding the first substrate and the second substrate, and which includes an opening corresponding to the detection well.

[0038] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a substrate material and a detection reagent distributed within the substrate material, and the substrate material includes glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber.

[0039] For example, a detection chip provided in at least one embodiment of the present disclosure further includes a first substrate having a first surface, wherein the sample supply port is a through-hole of the first substrate, and the flow guide groove and the detection well are formed on the first surface of the first substrate, and a second substrate stacked on the first surface of the first substrate and having a detection through-hole at a position corresponding to the detection well, enabling the optical inspection through the detection through-hole, wherein the at least one detection branching structure includes a plurality of detection branching structures, and the plurality of detection branching structures are evenly arranged around the sample supply port.

[0040] For example, a detection chip provided in at least one embodiment of the present disclosure further includes a first substrate having opposing first and second surfaces, wherein the sample supply port is a through-hole of the first substrate, the detection well is formed on the first surface of the first substrate, and the flow guide groove is formed on the second surface of the first substrate; a second substrate stacked on the first surface of the first substrate and enabling the optical inspection at a position corresponding to the detection well; and a third substrate stacked on the second surface of the first substrate and sealing the sample supply port and the flow guide groove.

[0041] For example, the detection chip provided in at least one embodiment of the present disclosure further includes a first substrate having a first surface, wherein the sample supply port, the flow guide groove, and the detection well are formed on the first surface of the first substrate, and the sample supply port is a non-through hole of the first substrate; and a second substrate stacked on the first surface of the first substrate, exposing the sample supply port and enabling the optical inspection at a position corresponding to the detection well.

[0042] For example, the detection chip provided in at least one embodiment of the present disclosure further includes an optical calibration branching structure, the optical calibration branching structure including an optical path detection area, and the optical path detection area configured to perform optical calibration.

[0043] For example, the detection chip provided in at least one embodiment of the present disclosure further includes a sample supply convex portion, which protrudes from the first surface of the first substrate in a direction away from the second substrate, and one end of the sample supply convex portion is connected to the sample supply port.

[0044] For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample supply convex portion includes a conical cavity, the volume of the conical cavity is 50 μL to 200 μL, the height of the conical cavity protruding from the first substrate is 1 mm to 20 mm, the conical cavity has a first end and a second end opposite to each other, the first end is connected to the sample supply port, the diameter of the first end is 0.5 mm to 5 mm, and the diameter of the second end is 1 mm to 20 mm.

[0045] For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate includes a first recess, the second substrate includes a second recess corresponding to the first recess, and the first recess and the second recess are used to fix the detection chip.

[0046] At least one embodiment of the present disclosure also provides a detection system including a detection device configured to detect the reaction reagent in the detection well by the detection unit, and a detection chip described in any embodiment of the present disclosure.

[0047] For example, in the detection system provided in at least one embodiment of the disclosure, the detection device includes a light source configured to emit light to the reaction reagent, and a photoelectric detection device configured to receive light emitted from the light source and reflected by the reaction reagent.

[0048] In order to more clearly describe the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly described below. Obviously, the drawings in the following description are only some embodiments of the present disclosure and are not intended to limit the present disclosure. [Brief explanation of the drawings]

[0049] [Figure 1] 1A and 1B are front and back perspective views, respectively, of a detection chip provided in at least one embodiment of the present disclosure. [Figure 2]2A and 2B are exploded views of the detection chip shown in FIGS. 1A and 1B, respectively. [Figure 3] 3A and 3B are front and back perspective views, respectively, of the first substrate of the detection chip shown in FIGS. 1A and 1B. [Figure 4] FIG. 4 is a schematic plan view of the structure of the first surface of the first substrate of the detection chip shown in FIGS. 1A and 1B. [Figure 5] FIG. 5 is a schematic plan view of the second substrate of the detection chip shown in FIGS. 1A and 1B. [Figure 6] FIG. 6 is a structural schematic diagram of the reaction film of the detection chip shown in FIGS. 1A and 1B. [Figure 7] 7A and 7B are front and back perspective views, respectively, of another sensing chip provided in at least one embodiment of the present disclosure. [Figure 8] FIG. 8 is an exploded view of the detection chip shown in FIGS. 7A and 7B. [Figure 9] 9A and 9B are front and back perspective views, respectively, of the first substrate of the detection chip shown in FIGS. 7A and 7B. [Figure 10] FIG. 10 is a schematic plan view of the structure of the first surface of the first substrate of the detection chip shown in FIGS. 7A and 7B. [Figure 11] FIG. 11 is an exploded view of a further sensing chip provided in at least one embodiment of the present disclosure. [Figure 12] FIG. 12 is a structural schematic diagram of the first substrate of the detection chip shown in FIG. [Figure 13A] 13A is a schematic diagram of a portion of the structure of the first substrate of the detection chip shown in FIG. [Figure 13B] FIG. 13B is a schematic cross-sectional view taken along line AA' in FIG. 13A. [Figure 13C] FIG. 13C is a schematic cross-sectional view taken along line BB' in FIG. 13A. [Figure 14] FIG. 14 is an exploded view of a further sensing chip provided in at least one embodiment of the present disclosure. [Figure 15]15A and 15B are exploded views of a further sensing chip provided in at least one embodiment of the present disclosure. [Figure 16] FIG. 16 is an exploded view of a further sensing chip provided in at least one embodiment of the present disclosure. [Figure 17] FIG. 17 is a schematic diagram of a detection system provided in at least one embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0050] In order to clarify the objectives, technical solutions and advantages of the embodiments of the present disclosure, the technical solutions of the embodiments of the present disclosure will be described clearly and completely below with reference to the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are only a part of the embodiments of the present disclosure, but not all of the embodiments. All other embodiments obtained by those skilled in the art based on the described embodiments of the present disclosure without requiring creative efforts belong to the patent scope of the present disclosure.

[0051] Unless otherwise defined, technical or scientific terms used in this disclosure have the common meaning understood by those skilled in the art. The terms "first," "second," and similar terms used in this disclosure do not denote any order, number, or importance, but merely distinguish between different components. Similar terms such as "comprise" or "comprises" mean that the component or item listed before the term covers the component or item listed after the term or its equivalents, and does not exclude other components or items. Similar terms such as "connected" or "coupled" are not limited to physical or mechanical connections, but include electrical connections, whether direct or indirect. Terms such as "top," "bottom," "left," and "right" only indicate relative positions, and if the absolute position of the described object changes, this relative position may change accordingly.

[0052] When designing a microfluidic chip, the inventors hope to integrate as many analytical and detection functions as possible into the chip, reduce the chip's dependence on external operations, and achieve automation and integration. For example, the sample injection component and analytical and detection component of the microfluidic chip can be integrated into one body, and the structural design can achieve automation of the detection process, further reducing the technical requirements for operators, reducing human error, and making the obtained detection data more accurate.

[0053] At least one embodiment of the present disclosure provides a detection chip including a sample supply port and at least one detection branch structure, each of which includes a detection well communicating with the sample supply port and a reaction reagent contained in the detection well, and a detection unit configured to enable optical inspection of the reaction reagent in the detection well.

[0054] In the present disclosure, the detection chip provided in at least one of the above embodiments integrates multiple basic structural units or components for sample detection on the same chip, and completes the entire process of sample analysis detection through active control and capillary action, thereby realizing the automation and integration of the detection process, thereby reducing artificial errors that may exist in the detection process, improving the accuracy of detection data, and realizing a thinner or smaller overall detection chip, which is advantageous for realizing a portable detection system.

[0055] The detection chip and detection system provided in the present disclosure will be described below through several specific examples.

[0056] In some embodiments of the present disclosure, at least one detection branch structure further includes a flow channel having a first end and a second end, the first end of the flow channel being connected to the sample supply port, and the second end of the flow channel being connected to the detection well, whereby the detection well is connected to the sample supply port via the flow channel.

[0057] 1A and 1B are front and back perspective views, respectively, of a sensing chip provided in at least one embodiment of the present disclosure, and FIGS. 2A and 2B are exploded views, i.e., exploded views, of the sensing chip shown in FIGS. 1A and 1B. FIGS. 1A and 2A are top views of the sensing chip showing the structure of the sensing chip as seen from the front, and FIGS. 1B and 2B are bottom views of the sensing chip showing the structure of the sensing chip as seen from the back.

[0058] 1A to 2B, the detection chip 10 includes a sample supply port 110 and at least one detection branching structure 120, for example, a plurality of detection branching structures 120, and six detection branching structures 120 are illustrated in the drawings as an example. The sample supply port 110 supplies a sample to be detected, such as breast milk, body fluid, or blood. The plurality of detection branching structures 120 are evenly arranged around the sample supply port 110, so that each of the plurality of detection branching structures 120 can independently perform a detection function.

[0059] For example, each of the multiple detection branch structures 120 includes a flow channel 130 having a first end and a second end communicating with the sample supply port 110, a detection well 141 communicating with the second end of the flow channel 130, and a detection section 140 configured to include a reaction reagent 150 contained in the detection well 141 and to enable optical inspection of the reaction reagent 150 in the detection well 141.

[0060] For example, in some embodiments, the reaction reagent 150 may be in the form of a sheet, i.e., a reaction film, as shown in Figures 1A to 2B, or in some embodiments, the reaction reagent 150 may be in the form of a bulk or powder reaction reagent, such as a bulk or powder freeze-dried reagent, or in some embodiments, for example, when the detection chip includes multiple detection branch structures, the reaction reagent contained in some of the detection wells may be in the form of a sheet-like reaction film, and the reaction reagent contained in the remaining detection wells may be different from the sheet-like reaction film and may be, for example, in the form of a bulk or powder reaction reagent, and the embodiments of the present disclosure are not limited thereto.

[0061] Hereinafter, the detection chip provided in the embodiment of the present disclosure will be described taking the case where the reaction reagent 150 is a reaction film (i.e., reaction film 150) as an example. Note that the embodiment of the present disclosure includes, but is not limited to, the above examples.

[0062] For example, in some embodiments, the detection chip further includes an optical calibration branch structure including an optical path detection region configured to perform optical calibration, thereby improving the accuracy and precision of the detection data obtained by optical inspection.

[0063] For example, when the detection chip includes multiple detection branch structures, the optical calibration branch structure and the multiple detection branch structures are evenly arranged around the sample supply port. The optical calibration branch structure does not have a reaction film. For example, in the optical calibration branch structure, all other structures other than the reaction film may be substantially the same or similar to those of the detection branch structure, so that the optical calibration branch structure and the detection branch structure may be integrated, simplifying the manufacturing process of the detection chip and reducing manufacturing costs.

[0064] For example, taking the detection chip 10 shown in Figures 1A to 2B as an example, one detection branching structure 120 does not have a reaction film 150 and may be used for optical calibration as an optical calibration branching structure of the detection chip 10; for example, one detection branching structure 120 shown in Figure 2B does not have, for example, a reaction film 150 (and a water-absorbing film 170 described later) and functions as an optical calibration branching structure of the detection chip 10.

[0065] For example, the optical calibration branching structure may include an optical path detection region, which may be located at a position corresponding to the detection well 141. In this way, when using a detection device to optically inspect the reaction film 150 of the detection chip 10, the optical calibration branching structure can calibrate the optical path emitted from the detection device, for example, by calibrating the irradiation angle of the optical path, thereby improving the accuracy of the obtained detection data.

[0066] In some embodiments of the present disclosure, two or more optical calibration branching structures may be provided for optical calibration as needed. For example, taking the detection chip 10 shown in Figures 1A to 2B as an example, the two detection branching structures 120 may be optical calibration branching structures of the detection chip 10 without the reaction film 150, and these two optical calibration branching structures may be arranged point-symmetrically with respect to the sample supply port 110, thereby improving the accuracy and precision of the optical calibration and further improving the accuracy of the obtained detection data.

[0067] It should be noted that in some other embodiments of the present disclosure, the detection chip may be calibrated in other ways as needed, and in such cases, the calibration branch structure may be a calibration structure of other types than optical calibration. For example, in other types of calibration structures, a reaction film (or other structures or components that affect the calibration, such as the water-absorbing film 170 described below) may or may not be provided as needed, and the embodiments of the present disclosure are not limited thereto.

[0068] For example, the reaction film 150 contains a detection reagent, and the sample to be detected added through the sample supply port 110 enters the detection well 141 through the flow guiding groove 130 and reacts with the detection reagent in the reaction film 150. At this time, by detecting, for example, a color change that appears on the reaction film 150 after the reaction between the detection reagent and the sample to be detected, for example, by optical inspection, it is possible to detect the presence or absence or content of a certain component in the sample to be detected.

[0069] Thus, the detection chip 10 provided in at least one embodiment of the present disclosure integrates multiple functions, such as mixing and analytical detection, and completes the entire sample analysis and detection process through active control and capillary action, thereby realizing automation and integration of the detection process, reducing artificial errors that may exist in the detection process, and improving the accuracy of the detection data. Furthermore, in some embodiments, the detection chip 10 can simultaneously detect multiple indicators of the same sample using different detection reagents by placing reaction films 150 containing different detection reagents in each of the detection wells 141 of the multiple detection branch structures 120, thereby shortening the sample detection cycle and timely detecting multiple indicators of the sample.

[0070] For example, in some instances, the sample to be detected may be a liquid, such as a breast milk sample. In manufacturing the detection chip 10, the initial reaction film 150 may be titrated with a desired detection reagent (e.g., a color-developing reagent) in advance. The detection reagent may then be impregnated into the reaction film 150 and dried. The reaction film 150 containing the detection reagent may then be placed in the detection well of the detection chip 10, which is advantageous for storage and transportation of the detection chip 10. When performing detection using the detection chip 10, the sample is introduced through the sample supply port 110, flows through the flow channel 130 into the detection well 141, and mixes with the detection reagent in the reaction film 150, causing a reaction. Thus, by detecting the reaction result (e.g., by identifying a color change in the reaction film 150), the presence or amount of the analyte in the sample may be determined, or a certain indicator of the sample may be detected.

[0071] For example, in at least one of the above-described embodiments, the amount of sample injected may be 60 μL to 80 μL when detecting a sample using the detection chip 10. This reduces the amount of sample required when detecting a sample using the detection chip 10, thereby reducing or avoiding sample waste.

[0072] For example, in some embodiments, as shown in Figures 1A to 2B, each detection branch structure 120 may further include a water-absorbing membrane 170, which is contained in the detection well 141 and at least partially overlaps with the reaction film 150 in the thickness direction of the reaction film 150 (i.e., the direction perpendicular to the first substrate 101 described below), for example, the two are in direct contact with each other.

[0073] For example, the liquid storage capacity of each water-absorbing membrane 170 may be 10 μL to 50 μL, e.g., 30 μL. The water-absorbing membrane 170 can accommodate a certain amount of sample, and once the liquid storage capacity of the water-absorbing membrane 170 is exceeded, the water-absorbing membrane 170 will no longer absorb the sample, thereby serving to quantify the sample and being advantageous for controlling the sample amount.

[0074] For example, the material of the water-absorbing membrane 170 includes materials such as glass fiber, cotton fiber, or a composite of glass fiber and cotton fiber.

[0075] It should be noted that in some other embodiments of the present disclosure, the detection chip may not utilize a water-absorbing membrane, in which case the reaction film 150 serves the dual functions of sample quantification and reaction carrier.

[0076] In addition, in the embodiment of the present disclosure, the length, width, depth, etc. of the multiple flow channels 130 are all the same, which ensures that the amount of sample flowing into each detection well 141 is approximately the same, which is advantageous for controlling the amount of sample in the detection well 141.In addition, the reaction time between the sample in each detection well 141 and the detection reagent in the reaction film 150 can be made relatively consistent, thereby improving the accuracy of the obtained detection data.

[0077] In some other embodiments of the present disclosure, for example, when multiple indicators of the same sample are detected simultaneously using different detection reagents, the sizes of the multiple flow channels 130 may be different, for example, different lengths, widths, depths, etc., depending on the indicators of the sample that need to be detected. This allows the amount of sample in the different detection wells 141 and the reaction time between the sample in the different detection wells 141 and the detection reagent in the reaction film 150 to be controlled, and more accurate multiple detection data can be obtained simultaneously as needed.

[0078] In the embodiment of the present disclosure, the specific length, width, depth, etc. of the flow guiding channel 130 may be determined according to actual needs, such as the amount of sample, sample characteristics, etc. For example, as long as the sample can flow from the sample supply port 110 into the detection well 141, the length of the flow guiding channel 130 may be appropriately reduced to reduce or avoid loss of the sample due to flow.

[0079] In some other embodiments of the present disclosure, the detection chip 10 may not be provided with the flow guide groove 130, and for example, the detection wells 141 and the sample supply port 110 may be directly connected and communicated with each other, thereby further reducing or avoiding sample loss due to flow. In this case, in some examples, the sample supply port 110 may have a flow guide structure that communicates with multiple detection wells 141, respectively, so that the sample added to the sample supply port 110 can flow uniformly into the multiple detection wells 141.

[0080] For example, in some embodiments, the size of the plurality of detection wells 141 may be determined according to various indicators of the sample to be detected, the amount of sample required, the type of detection reagent, etc. The sizes and shapes of the plurality of detection wells 141 may be the same or different as needed, and the embodiments of the present disclosure are not limited thereto.

[0081] For example, in some embodiments, the detection chip 10 further includes a first substrate 101 and a second substrate 102. The first substrate 101 has opposing first and second surfaces, the sample supply port 110 is a through-hole in the first substrate 101, and the flow guide groove 130 and the detection well 141 are formed on the first surface of the first substrate 101. The second substrate 102 is laminated on the first surface of the first substrate 101 to enable optical inspection at a position corresponding to the detection well 141.

[0082] For example, Figures 3A and 3B are front and back perspective views, respectively, of the first substrate of the detection chip shown in Figures 1A and 1B. Figure 3A is a top view of first substrate 101 showing the front structure of the first substrate, and Figure 3B is a bottom view of first substrate 101 showing the back structure of the first substrate. Figure 4 is a schematic planar structure diagram of the first surface of the first substrate of the detection chip shown in Figures 1A and 1B, and Figure 5 is a schematic planar structure diagram of the second substrate of the detection chip shown in Figures 1A and 1B.

[0083] For example, as shown in Figures 1A to 5, if the detection chip has a water-absorbent film 170, the water-absorbent film 170 may be provided on the side of the reaction film 150 away from the second substrate 102, i.e., between the reaction film 150 and the detection well 141.

[0084] For example, the second substrate 102 has a detection through-hole 160 at a position corresponding to the detection well 141, which allows optical inspection of the reaction film 150 in the detection well 141 through the detection through-hole 160 to obtain a detection indicator for the sample.

[0085] For example, the detection through-holes 160 may penetrate the second substrate 102, and the diameter of the detection through-holes 160 may be set to 2 mm to 10 mm, or further 0.5 mm to 4 mm, for example 3 mm. This allows optical inspection to be performed through the detection through-holes 160, and reduces or avoids interference between the lights emitted to the respective detection through-holes 160, thereby further improving the accuracy and precision of the optical inspection.

[0086] For example, the entire portion of the second substrate 102 other than the detection through-holes 160 may be made non-transparent, thereby reducing or avoiding interference between the lights emitted to the respective detection through-holes 160 and reducing or avoiding optical crosstalk between the detection through-holes 160. For example, the opaque portion of the second substrate 102 may be formed by dyeing or the like.

[0087] For example, a light-shielding layer may be provided on the side of the second substrate 102 away from the first substrate 101, and the light-shielding layer covers the surface of the second substrate 102 away from the first substrate 101 except for the positions where the detection through-holes 160 are located, i.e., the light-shielding layer covers the surface of the second substrate 102 away from the first substrate 101 and exposes the detection through-holes 160, thereby reducing or avoiding interference between the lights emitted to the respective detection through-holes 160 and reducing or avoiding optical crosstalk between the detection through-holes 160. For example, the light-shielding layer may be made of an opaque material and provided on the surface of the second substrate 102 by, for example, adhesion, printing, etc., and the embodiments of the present disclosure do not limit the specific method of forming the light-shielding layer.

[0088] For example, the light-shielding layer may be provided on the side of the second substrate 102 that is close to the first substrate 101, i.e., the light-shielding layer covers the surface of the second substrate 102 that is close to the first substrate 101 and exposes the detection through holes 160, thereby reducing or avoiding interference between the light beams emitted toward each of the detection through holes 160. Alternatively, in some embodiments of the present disclosure, light-shielding layers may be provided on both the side of the second substrate 102 that is away from the first substrate 101 and the side of the second substrate 102 that is close to the first substrate 101, and these two light-shielding layers cover portions of the surfaces of the second substrate 102 that are close to and away from the first substrate 101 other than the positions where the detection through holes 160 are located, thereby better reducing or avoiding interference between the light beams emitted toward each of the detection through holes 160 and further reducing or avoiding optical crosstalk between the detection through holes 160.

[0089] For example, in some other embodiments of the present disclosure, the second substrate of the detection chip may not have a detection through-hole; in this case, the second substrate is made of a transparent material that can transmit light, thereby allowing optical inspection of the reaction film in the detection well directly through the second substrate.

[0090] For example, the second substrate may be made of a transparent material that allows light to pass through, and an opaque light-shielding layer may be printed or adhered to the surface of the second substrate away from the first substrate to form a detection window, allowing light to pass only through the detection window. For example, in some embodiments, the light-shielding layer may be provided on the surface of the second substrate that faces the first substrate, or on both the surface of the second substrate that faces the first substrate and the surface that faces away from the first substrate; embodiments of the present disclosure are not limited thereto. Embodiments of the present disclosure are not limited to a specific material for the second substrate, as long as the reaction film in the detection well of the first substrate is optically inspected through the second substrate or a detection through-hole opened in the second substrate.

[0091] In the embodiment of the present disclosure, the number of detection wells 141 provided in the first substrate 101 and the number of detection through-holes 160 provided in the second substrate 102 corresponding to the detection wells 141 are merely illustrative, and the embodiment of the present disclosure does not limit the specific numbers of detection wells 141 and detection through-holes 160. For example, 1 to 20 detection wells 141 are provided in the first substrate 101, and in such a case, 1 to 20 detection through-holes 160 are provided in the second substrate 102.

[0092] For example, the material of the second substrate 102 may include one or more of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate. For example, the material of the first substrate 101 may include one or more of polymethyl methacrylate, polystyrene, and polycarbonate, or may be other materials having high light transmittance. In the embodiment of the present disclosure, the materials of the first substrate 101 and the second substrate 102 are not particularly limited.

[0093] For example, the first substrate 101 and the second substrate 102 may be bonded together by bonding (a technique in which two materials are bonded together using van der Waals forces, molecular forces, atomic forces, etc.), welding, adhesion, or clinging, thereby simplifying processes such as processing and assembly of the detection chip 10 and reducing the manufacturing cost of the detection chip 10. For example, the first substrate 101 and the second substrate 102 may be bonded together by processes such as ultrasonic bonding, hot press bonding, laser welding, ultrasonic welding, and silicone sealing. The embodiments of the present disclosure are not limited to specific means for bonding the first substrate 101 and the second substrate 102.

[0094] For example, the first substrate 101 may be circular, rectangular, or any other suitable shape, and the second substrate 102 may similarly be circular, rectangular, or any other suitable shape.

[0095] For example, if the first substrate 101 is circular, the diameter of the first substrate 101 may be 3 cm to 15 cm, or even 3 cm to 5 cm. Because the detection chip 10 is used in combination with, for example, a detection device, the first substrate 101 having the above dimensions appropriately reduces the space occupied by the detection chip 10 and enables the detection chip 10 to be thinned, which contributes to the combined use of the detection chip 10 and the detection device, and reduces costs for transporting, packaging, and storing the detection chip 10. For example, if the second substrate 102 is circular, the diameter of the second substrate 102 may be 3 cm to 15 cm, or even 3 cm to 5 cm. Because the detection chip 10 is used in combination with, for example, a detection device, the second substrate 102 having the above dimensions appropriately reduces the space occupied by the detection chip 10 and enables the detection chip 10 to be thinned, which contributes to the combined use of the detection chip 10 and the detection device, and reduces costs for transporting, packaging, and storing the detection chip 10.

[0096] For example, if the first substrate 101 is rectangular, the diagonal of the first substrate 101 may be 3 cm to 15 cm, or even 3 cm to 5 cm. For example, a rectangular first substrate 101 simplifies the manufacturing process of the detection chip 10, reduces the requirements for the processing and precision of the detection chip 10, and can reduce the manufacturing cost of the detection chip 10. If the second substrate 102 is rectangular, the diagonal of the second substrate 102 may be 3 cm to 15 cm, or even 3 cm to 5 cm. For example, a rectangular second substrate 102 simplifies the manufacturing process of the detection chip 10, reduces the requirements for the processing and precision of the detection chip 10, and can reduce the manufacturing cost of the detection chip 10.

[0097] For example, the first substrate 101 and the second substrate 102 may have the same or similar shapes and sizes to facilitate bonding between the first substrate 101 and the second substrate 102.

[0098] For example, the thickness of the first substrate 101 may be 0.5 mm to 10 mm, such as 3 mm or 5 mm. For example, the thickness of the second substrate 102 may be 0.1 mm to 5 mm, such as 0.5 mm or 2 mm. This appropriately reduces the space occupied by the detection chip 10 and enables the detection chip 10 to be made thinner, thereby reducing costs for transporting, packaging, storing, etc. of the detection chip 10 and contributing to the use of the detection chip 10 in combination with a detection device. Furthermore, the above numerical ranges contribute to the processing and manufacturing of the detection chip 10 and make it easier for a user to hold the device in one hand and use it to fix or pick up the device.

[0099] For example, in one example, the diameters of the first substrate 101 and the second substrate 102 are 3.5 cm, the thickness of the first substrate 101 is 1.5 mm, and the thickness of the second substrate 102 is 1 mm. This allows the detection chip 10 to be made thinner, making it easier for a user to hold the device in one hand, for example, for use, fixation, and picking up. It also simplifies the manufacturing process of the detection chip 10 and reduces the requirements for the processing process and precision of the detection chip 10, contributing to the processing and manufacturing of the detection chip 10.

[0100] 1A to 5, in some embodiments, the detection chip 10 may further include a sample supply convex 190. The sample supply convex 190 protrudes from the first surface of the first substrate 101 in a direction away from the second substrate 102, and one end of the sample supply convex 190 may be connected to the sample supply port 110 and the other end may be connected to the atmosphere. The sample supply convex 190 serves to accommodate and guide the sample, and can allow the sample to flow quickly through the sample supply convex 190 to the sample supply port 110, thereby facilitating the injection of the sample into the sample supply port 110, increasing the sample volume of the detection chip 10, and being advantageous for, for example, picking up and setting the detection chip 10.

[0101] For example, the sample supply convex 190 includes a conical cavity and connects the sample supply port 110 to the atmosphere. For example, the conical cavity may have a cone angle (e.g., the angle between the center line of the cone and the sidewall) of 30° to 75° and a volume of 50 μL to 200 μL. For example, the conical cavity of the sample supply convex 190 includes a first end and a second end facing each other. The first end is connected to the sample supply port 110 and has a diameter of 0.5 mm to 5 mm, and the second end has a diameter of 1 mm to 20 mm. The height of the conical cavity of the sample supply convex 190 protruding from the first substrate 101 is 1 mm to 20 mm. This allows the sample supply convex 190 to occupy an appropriate space to match the size of the detection device when the detection chip 10 is used in combination with, for example, a detection device, contributing to the combined use of the detection chip 10 and the detection device. Furthermore, the sample supply convex portion 190 determined by the above numerical range can better perform the role of accommodating and directing the sample, for example, allowing the sample to flow into the sample supply port 110 at a uniform and reasonable speed, thereby contributing to the injection of the sample into the sample supply port 110.

[0102] For example, in one example, the diameter of one end of the sample supply convex portion 190 connected to the sample supply port 110 is 3 mm, the diameter of the other end is 10 mm, and the height of the sample supply convex portion 190 protruding from the first substrate 101 is 1.5 mm. This allows the sample to flow quickly to the sample supply port 110 through the sample supply convex portion 190 and is advantageous for use in combination with, for example, a pickup or set of the detection chip 10 or a detection device. In addition, the manufacturing process of the sample supply convex portion 190 can be simplified, reducing the requirements for the processing process and precision.

[0103] For example, in some embodiments, as shown in FIG. 4, the sample supply port 110 includes a first main body 111 and a first protrusion 112 that protrudes from the first main body 111 into the flow guide groove 130, and the first protrusion 112 is connected to the flow guide groove 130 so that the sample flows quickly into the flow guide groove 130 through the first protrusion 112.

[0104] For example, the diameter of the first body 111 may be 1 mm to 10 mm, such as 5 mm or 7 mm, which ensures that the sample flows uniformly and quickly into the flow guiding channel 130 and also ensures that the space required for the detection well 141 is sufficient.

[0105] For example, the shape of the first body 111 may be circular, square, or other suitable shape, and the first protrusion 112 may be square, have sharp corners, or other shapes, and the embodiments of the present disclosure are not limited thereto. For example, if the shape of the first body 111 is rectangular, the diagonal length of the first body 111 may be 1 mm to 10 mm, such as 5 mm or 7 mm. For example, a circular or rectangular first body 111 can simplify the manufacturing process of the detection chip 10 and reduce the requirements for the processing process and precision of the detection chip 10, thereby reducing the manufacturing cost of the detection chip 10.

[0106] For example, the inner wall of the flow guiding groove 130 may be hydrophilic, e.g., the contact angle of the liquid on the inner wall of the flow guiding groove 130 is less than 90°, which contributes to the rapid flow of the sample into the flow guiding groove 130 and allows the sample to quickly flow into the detection well 141 through the flow guiding groove 130 and mix with the reaction film 150, further shortening the sample detection time and improving the accuracy of the obtained detection data. For example, in manufacturing a detection chip, a hydrophilic treatment agent is injected into the flow guiding groove 130, the flow guiding groove 130 is immersed in the hydrophilic treatment agent for 1 minute, and then the hydrophilic treatment agent is expelled with air, thereby making the inner wall of the flow guiding groove 130 hydrophilic.

[0107] The contact angle of the liquid on the inner wall of the guiding groove 130 is the angle between a tangent to the surface of the droplet and the surface of the inner wall of the guiding groove 130. For example, if the contact angle of the liquid on the inner wall of the guiding groove 130 is less than 90°, the inner wall of the guiding groove 130 is considered to be hydrophilic. The smaller the contact angle of the liquid on the inner wall of the guiding groove 130, the better the wettability of the inner wall of the guiding groove 130. For example, if the contact angle of the liquid on the inner wall of the guiding groove 130 is 0°, the material of the inner wall of the guiding groove 130 is completely wetted. The embodiments of the present disclosure do not limit the specific value of the contact angle of the liquid on the inner wall of the guiding groove 130; it is sufficient for the inner wall of the guiding groove 130 to be hydrophilic if the contact angle is less than 90°.

[0108] For example, in some embodiments, the height of the flow guiding channel 130 may be 0.1 mm to 1.5 mm, e.g., 0.5 mm, and the width of the flow guiding channel 130 may be 0.1 mm to 2 mm, e.g., 0.5 mm, which helps control the amount of sample flowing into the detection well 141 through the flow guiding channel 130 and reduces or prevents sample loss due to flow.

[0109] For example, in some embodiments, the ratio of the height of the flow guiding groove 130 to the width of the flow guiding groove 130 may be 1:1 to 10:1, for example, 2:1. Thus, in some embodiments, when the first substrate 101 and the second substrate 102 are bonded together by adhesion, for example, when the first surface of the first substrate 101 and the second substrate 102 are bonded together with an adhesive, the adhesive may be a hydrophobic material, and a hydrophobic adhesive may be present between the first surface of the first substrate 101 and the adhesive surface of the second substrate 102. Therefore, by increasing the ratio of the height of the flow guiding groove 130 to the width of the flow guiding groove 130, the contact area of ​​the adhesive surface of the second substrate 102 with the hydrophobic adhesive as the sample flows through the flow guiding groove 130 can be reduced, and the sample will flow faster through the flow guiding groove 130.

[0110] For example, in one example, the height of the guide channel 130 is 1 mm, and the width of the guide channel 130 is 0.5 mm, which is advantageous for enabling the sample to flow quickly through the guide channel 130, reducing or avoiding loss of the sample due to flow, and also simplifying the manufacturing process of the guide channel 130, reducing the requirements for the processing process and precision.

[0111] In some embodiments, the second end of the flow channel 130 (i.e., the end communicating with the detection well 141) contacts the reaction film 150, thereby contributing to the impregnation of the sample into the reaction film 150 and allowing the sample to fully react with the detection reagent in the reaction film 150; for example, a portion of the reaction film 150 may be located within the flow channel 130. In some embodiments, the reaction film 150 may not contact the second end of the flow channel 130, and the embodiments of the present disclosure are not limited thereto.

[0112] For example, in the embodiments of the present disclosure, structures of the detection chip 10, such as the sample supply protrusion 190, the sample supply port 110, the flow guide groove 130, and the detection well 141, may be integrally formed, for example, by an injection molding process, thereby simplifying the manufacturing process of the detection chip 10.

[0113] For example, in some embodiments, the reactive film 150 includes a substrate material and a detection reagent distributed within the substrate material, which may include materials such as glass fiber, cotton fiber, or a composite of glass fiber and cotton fiber.

[0114] For example, in some detection chips according to embodiments, when the first substrate 101 and the second substrate 102 are bonded together, the reaction film 150 is compressed between the two substrates in the thickness direction. For example, the thickness of the reaction film 150 in the relaxed state may be equal to or slightly greater than the height of the detection well 141. For example, the ratio of the thickness of the reaction film 150 in the relaxed state to the height of the detection well 141 may be 1:1 to 1:0.5. Furthermore, after the first substrate 101 and the second substrate 102 are bonded together, the reaction film 150 is compressed in the thickness direction, so that the surface of the reaction film 150 facing the second substrate 102 may be flush with the first surface of the first substrate 101. This allows the reaction film 150 and the second substrate 102 to adhere closely to each other, thereby reducing or avoiding gaps between the reaction film 150 and the first substrate 101 or the second substrate 102, which can cause liquid to accumulate. This allows the introduced sample to be sufficiently impregnated into the reaction film 150 and to react more thoroughly with the detection reagent in the reaction film 150, thereby improving the accuracy of the obtained detection data.

[0115] For example, in some embodiments of the present disclosure, the compression amount of the reaction film 150 may be 10% to 40%, which is advantageous for adhering the reaction film 150 to the second substrate 102 and reducing or avoiding gaps between the reaction film 150 and the first substrate 101 or the second substrate 102 that may cause liquid accumulation.

[0116] For example, the shape of the reaction film 150 is typically the same as or similar to the shape of the detection well 141 so that the reaction film 150 is received within the detection well 141 and contributes to uniform mixing of the reaction film 150 and the sample.

[0117] For example, FIG. 6 shows an exemplary structure of the reaction film of the detection chip shown in FIGS. 1A and 1B.

[0118] 4 and 6, in some embodiments, the reaction film 150 includes a film body 151 and a second convex portion 152. The film body 151 has a shape similar to that of the detection well 141, and both are circular. The second convex portion 152 protrudes from the film body 151 into the flow guiding groove 130, and at least a portion of the second convex portion 152 is located in the flow guiding groove 130. This allows the sample to be guided from the flow guiding groove 130 into the detection well 141, and the sample can be sufficiently impregnated into the reaction film 150, contributing to the sample reacting sufficiently with the detection reagent in the reaction film 150 and improving the accuracy of the obtained detection data.

[0119] For example, the diameter of the detection well 141 may be 3 mm to 15 mm, and the diameter of the film body 151 of the reaction film 150 is equal to or smaller than the diameter of the detection well 141. This allows the reaction film 150 to be better accommodated in the detection well 141, contributing to uniform mixing of the reaction film 150 and the sample.

[0120] For example, in some other embodiments of the present disclosure, the reaction film may have a shape similar to the overall detection well, i.e., the reaction film may have only the film body portion and may not have a convex portion (e.g., second convex portion 152), and the embodiments of the present disclosure are not limited in this regard.

[0121] For example, in some other embodiments of the present disclosure, the reaction film may be polygonal, and one corner of the polygon may be directly connected to the second end of the flow channel. In such cases, the detection well may be polygonal in shape, the same as or similar to the shape of the reaction film.

[0122] For example, the reaction film may have a regular shape such as a triangle, a square, a diamond, or an irregular shape, and the embodiments of the present disclosure are not limited thereto.

[0123] For example, Figures 7A and 7B are front and back perspective views, respectively, of another sensing chip provided in at least one embodiment of the present disclosure. For example, Figure 7A is a top view of the sensing chip, and Figure 7B is a bottom view of the sensing chip. Figure 8 is an exploded view, i.e., an exploded view, of the sensing chip shown in Figures 7A and 7B.

[0124] 7A to 8, the detection chip 20 includes a sample supply port 210 and a plurality of detection branching structures 220, which are evenly arranged around the sample supply port 210. Each of the detection branching structures 220 includes a flow guiding groove 230 and a detection unit 240. The flow guiding groove 230 has a first end and a second end, and the first end is connected to the sample supply port 210. The detection unit 240 includes a detection well 241 and a reaction reagent (e.g., a reaction film 250), the detection well 241 is connected to the second end of the flow guiding groove 230, the reaction film 250 is contained in the detection well 241, and the detection unit 240 is configured to enable optical inspection of the reaction film 250 in the detection well 241.

[0125] For example, as shown in FIGS. 7A to 8, the reaction film 250 of the detection chip 20 is polygonal, such as a square or diamond, and accordingly, the shape of the detection well 241 is also square or diamond.

[0126] For example, the side length of the detection well 241 may be 3 mm to 15 mm, e.g., 5 mm or 10 mm, and the side length of the reaction film 250 is equal to or shorter than the side length of the detection well 241. This allows the reaction film 250 to be more easily accommodated within the detection well 241, contributing to uniform mixing of the reaction film 250 and the sample.

[0127] For example, if the detection well 341 is square, the diagonal of the square may be 1 mm to 20 mm, e.g., 10 mm or 15 mm. For example, if the detection well 341 is diamond-shaped, the two diagonals of the diamond may each be 1 mm to 20 mm, e.g., one diagonal is 10 mm long and the other is 6 mm long. For example, the ratio of the lengths of the two diagonals of the diamond may be 1:1 to 1:2. For example, the angle between two adjacent sides of the diamond may be 60° or less, which allows the sample to be sufficiently impregnated into the reaction film 250 along the periphery thereof and contributes to sufficient reaction with the detection reagent in the reaction film 250, further improving the accuracy of the obtained detection data.

[0128] For example, one corner of the reaction film 250 is directly connected to the second end of the flow channel 230, which guides the sample from the flow channel 230 into the detection well 241, allowing the sample to be fully impregnated into the reaction film 250 and fully react with the detection reagent in the reaction film 250, thereby further improving the accuracy of the obtained detection data.

[0129] Figures 9A and 9B are respectively a front perspective view and a back perspective view of the first substrate of the detection chip shown in Figures 7A and 7B. For example, Figure 9A is a top view of first substrate 201, and Figure 9B is a bottom view of first substrate 201. Figure 10 is a schematic planar structural view of the first surface of the first substrate of the detection chip shown in Figures 7A and 7B.

[0130] 7A to 10, the detection chip 20 further includes a first substrate 201 and a second substrate 202. The first substrate 201 has a first surface and a second surface facing each other, the sample supply port 210 is a through-hole in the first substrate 201, and the flow guide groove 230 and the detection well 241 are formed on the first surface of the first substrate 201. The second substrate 202 is stacked on the first surface of the first substrate 201, and enables optical inspection at a position corresponding to the detection well 241.

[0131] For example, the second substrate 202 has a detection through-hole 260 at a position corresponding to the detection well 241, and the reaction film 250 in the detection well 241 can be optically inspected through the detection through-hole 260 to obtain each detection indicator of the sample.

[0132] For example, in an embodiment of the present disclosure, the shape of the detection through-hole 260 is circular, and in some other embodiments of the present disclosure, the shape of the detection through-hole 260 may be, for example, square, diamond-shaped, or other shapes similar to the shape of the detection well 241, and the embodiments of the present disclosure are not limited in this regard.

[0133] For example, as shown in FIG. 10, the sample supply port 210 includes a first main body 211 and a first convex portion 212 that protrudes from the first main body 211 into the flow guide groove 230, the first convex portion 212 being connected to the flow guide groove 230, and the sample flows into the flow guide groove 230 via the first convex portion 212.

[0134] For the materials, specific structural parameters, etc. of the first substrate 201 and the second substrate 202, please refer to the corresponding descriptions of the first substrate 101 and the second substrate 102 of the detection chip 10 in the above embodiment, and they will not be described in detail here. For the specific structural parameters, materials, functions, etc. of the detection chip 20, please refer to the corresponding descriptions of the detection chip 10 in the above embodiment, and they will not be described in detail here.

[0135] For example, in some embodiments of the present disclosure, the detection portion of the detection chip may further include a flow-directing well positioned around the detection well, e.g., the flow-directing well may at least partially surround the detection well. The flow-directing well is formed on the first surface of the first substrate and communicates with the detection well, with at least some of the flow-directing wells having a height smaller than that of the detection well. This allows the sample to pass through the flow-directing well and quickly wet along a portion of the peripheral region of the reaction film, generating capillary force between the flow-directing well and the reaction film to guide the sample. This causes the sample to wet from the periphery of the reaction film to the central region of the reaction film, accelerating the mixing rate of the sample and the reaction film and further shortening the detection time. Furthermore, when the sample wets the reaction film from the periphery, capillary action increases the amount of sample wetted to the central region of the reaction film, making the detection result (e.g., color development) in the central region of the reaction film more obvious, facilitating subsequent observation of the detection result and improving the accuracy of the obtained detection data.

[0136] For example, in some embodiments of the present disclosure, the flow-directing well surrounds the detection well, i.e., surrounds the entire peripheral area of ​​the detection well, so that the sample passes through the flow-directing well and wets the reaction film faster along the periphery, and then wets from the periphery to the center of the reaction film, thereby accelerating the mixing speed of the sample and the reaction film and obviously further shortening the required detection time. Moreover, when the sample simultaneously wets the reaction film from the periphery, the amount of sample wetted to the center of the reaction film increases due to capillary action, making the detection result (e.g., color development) at the center of the reaction film most obvious, facilitating the observation of the subsequent detection result, which further improves the accuracy of the obtained detection data.

[0137] It should be noted that in some embodiments of the present disclosure, the flow guide wells may also intersect or partially overlap with the detection wells in a direction perpendicular to the first surface of the first substrate, and the embodiments of the present disclosure are not limited in this regard.

[0138] For example, Fig. 11 is an exploded view, i.e., an exploded view, of a further detection chip provided in at least one embodiment of the present disclosure. Fig. 12 is a structural schematic diagram of the first substrate of the detection chip shown in Fig. 11. For example, Fig. 12 is a bottom view of the first substrate 301 of the detection chip 30 shown in Fig. 11. Note that, except for the liquid storage through-hole 380 and the flow guide well 342, the detection chip 30 shown in Fig. 11 has substantially the same structure and function as the detection chip 20 shown in Figs. 7A to 8, and will not be described in detail here.

[0139] For example, as shown in FIGS. 11 and 12, the detection section 340 of the detection chip 30 includes a reaction reagent (for example, a reaction film 350 ), a detection well 341 , and flow guide wells 342 and 343 surrounding the detection well 341 .

[0140] Fig. 13A is a schematic diagram of a partial structure of the first substrate of the detection chip shown in Fig. 12, for example, a schematic diagram of a partial structure including the detection unit 340 of the first substrate 301 of the detection chip 30, Fig. 13B is a schematic diagram of a cross-sectional structure taken along line A-A' in Fig. 13A, and Fig. 13C is a schematic diagram of a cross-sectional structure taken along line B-B' in Fig. 13A. For simplicity and clarity, reaction film 350 of detection unit 340 is not shown in Figs. 12 to 13C.

[0141] 11 to 13C, the flow-directing wells 342 and 343 are formed on the first surface 3011 of the first substrate 301 and communicate with the detection well 341. For example, one end of the flow-directing well 342 is connected to the flow-directing groove 330, and the other end is connected to the flow-directing well 343. As a result, when the sample flows into the detection unit 340 through the flow-directing groove 330, the sample flows into the flow-directing wells 342 and 343, passes through the flow-directing wells 342 and 343, quickly wets the periphery of the reaction film 350, and then wets from the periphery to the center of the reaction film 350, accelerating the mixing speed of the sample and the reaction film 350 and shortening the required detection time. Furthermore, due to capillary action, the amount of sample wetted to the center of the reaction film 350 increases, making the detection result (e.g., color development) at the center of the reaction film 350 clearer, facilitating subsequent observation of the detection result and improving the accuracy of the obtained detection data.

[0142] For example, as shown in Figures 13A and 13B, the longitudinal cross sections of the flow well 342 and the detection well 341 are stepped as a whole, which creates a large gap for the sample to flow between the flow well 342 and the reaction film 350, making the flow rate of the sample in the flow well 342 even faster. In this way, the sample quickly wets along the periphery of the reaction film 350, and then wets from the periphery to the center of the reaction film 350, improving the wetting effect toward the center of the reaction film 350 and making the detection result (e.g., color development) at the center of the reaction film 350 clearer.

[0143] 13A and 13B, the height H2 of the flow guide well 342 is smaller than the height H1 of the detection well 341. For example, the height H1 of the detection well 341 may be 0.2 mm to 5 mm, such as 2 mm or 3 mm, so that when the first substrate 301 and the second substrate 302 are joined together, the reaction film 350 is compressed in the thickness direction by the two substrates, and the surface of the reaction film 350 facing the second substrate 302 may be flush with the first surface 3011 of the first substrate 301, so that the reaction film 350 and the second substrate 302 are in close contact with each other, thereby preventing a gap from forming between the reaction film 350 and the first substrate 301 or the second substrate 302 and causing liquid to accumulate. The difference between the height H2 of the flow guide well 342 and the height H1 of the detection well 341 may be 0.1 mm to 1 mm, for example, 0.5 mm or 0.8 mm, so that the sample can flow faster in the detection well 341 and wet the periphery of the reaction film 350 more quickly.

[0144] For example, the width D1 of the flow guiding well 342 may be 0.1 mm to 1 mm, e.g., 0.5 mm or 0.7 mm, which creates a sufficient and appropriate gap between the flow guiding well 342 and the reaction film 350 for the sample to flow, which is advantageous for the sample to wet from the periphery of the reaction film 350 to the center of the reaction film 350, improving the wetting effect at the center of the reaction film 350 and making the detection result (e.g., color development) at the center of the reaction film 350 clearer.

[0145] For example, if the detection well 341 is square, the diagonal of the square may be 1 mm to 20 mm, e.g., 10 mm or 15 mm. For example, if the detection well 341 is diamond-shaped, the two diagonals of the diamond may each be 1 mm to 20 mm, e.g., one diagonal is 10 mm long and the other is 6 mm long. For example, the ratio of the lengths of the two diagonals of the diamond may be 1:1 to 1:2. For example, the angle between two adjacent sides of the diamond may be 60° or less, which allows the sample to wet the reaction film 250 along the periphery toward the center of the reaction film 250, contributing to sufficient impregnation of the reaction film 250 and further improving the accuracy of the obtained detection data.

[0146] For example, in some other embodiments of the present disclosure, the flow guide well 342 may be stepped, including a multi-layer stepped structure, and the embodiments of the present disclosure are not limited in this regard.

[0147] 13A and 13C, the flow guide well 343 has an inclined structure. For example, the flow guide well 343 includes an inclined flow guide wall 344, which is inclined relative to the first surface 3011 of the first substrate 301, a first end 3441 of the flow guide wall 344 connected to the first surface 3011 of the first substrate 301, and a second end 3442 of the flow guide wall 344 connected to a side surface of the detection well 341. Since the gap through which the sample flows formed between the flow guide well 343 and the reaction film 350 is smaller than that of the flow guide well 342, the flow rate of the sample in the flow guide well 343 is slightly slower than that of the sample in the flow guide well 342. This reduces or prevents excessive sample from remaining in the flow well 343 after the sample flows through the flow well 342 into the flow well 343, and the speed at which the sample wets from the periphery of the reaction film 350 to the center of the reaction film 350 increases, making the detection result (e.g., color development) at the center of the reaction film 350 clearer, which is more advantageous for observing the subsequent detection results.

[0148] In some other embodiments of the present disclosure, the flow well 343 may have other suitable structures, as long as the space through which the sample flows formed between the flow well 343 and the reaction film 350 is equal to or smaller than the space through which the sample flows formed between the flow well 342 and the reaction film 350, and the embodiments of the present disclosure are not limited in this regard.

[0149] 12 , 13A, and 13C, the second end 3442 of the flow guiding wall 344 is not parallel to the first surface 3011 of the first substrate 301, but is inclined with respect to the first surface 3011 of the first substrate 301, and extends to the first surface 3011 of the first substrate 301 along the sample flow direction (e.g., the direction away from the sample supply port 310). In other words, the surface area of ​​the flow guiding wall 344 (i.e., the contact area between the flow guiding wall 344 and the sample) gradually decreases along the sample flow direction, for example, gradually decreases along the direction away from the sample supply port 310, and as a result, the space through which the sample flows formed between the flow guiding well 343 and the reaction film 350 gradually decreases along the sample flow direction. This increases the speed at which the sample wets from the periphery of the reaction film 350 to the center of the reaction film 350, further increasing the amount of sample wetted to the center of the reaction film 350, making the detection result (e.g., color development) at the center of the reaction film 350 clearer, which is more convenient for observing the subsequent detection results and improves the accuracy of the obtained detection data.

[0150] It should be noted that in some other embodiments of the present disclosure, the flow guide wall 344 may have other suitable contour shapes, and the contact area between the flow guide wall 344 and the sample may gradually decrease along the flow direction of the sample, and the embodiments of the present disclosure are not limited thereto.

[0151] For example, the maximum height H4 of the flow guide well 343 is smaller than the height H1 of the detection well 341, i.e., the distance between the second end of the flow guide wall 344 connected to the side of the detection well 341 and the first surface 3011 of the first substrate 301 is smaller than the height H1 of the detection well 341. This forms a gap through which the sample can flow between the flow guide well 343 and the reaction film 350, allowing the sample to quickly wet along the periphery of the reaction film 350 and then wet from the periphery to the center of the reaction film 350, thereby improving the wetting effect at the center of the reaction film 350 and making the detection result (e.g., color development) at the center of the reaction film 350 clearer.

[0152] For example, the difference between the distance between the second end of the flow guiding wall 344 and the first surface 3011 of the first substrate 301 and the height of the detection well 341 (i.e., the difference between H4 and H1) may be 0.1 mm to 1 mm, thereby forming an appropriate gap for the sample to flow between the flow guiding well 343 and the reaction film 350, thereby improving the wetting effect at the center of the reaction film 350.

[0153] For example, the width D2 of the flow well 343 may be 0.1 mm to 1 mm, such as 0.5 mm or 0.7 mm, thereby forming an appropriate gap for the sample to flow between the flow well 343 and the reaction film 350, thereby improving the wetting effect at the center of the reaction film 350.

[0154] It should be noted that in the above-described embodiments shown in Figures 11 to 13C, the width D1 of the flow well 342 and the width D2 of the flow well 343 are the same, and the height H2 of the flow well 342 and the maximum height H4 of the flow well 343 are the same; in some other embodiments of the present disclosure, the width D1 of the flow well 342 and the width D2 of the flow well 343 may be different, and the height H2 of the flow well 342 and the maximum height H4 of the flow well 343 may be different, and the embodiments of the present disclosure are not limited thereto.

[0155] In the above-described embodiments shown in Figures 11 to 13C, the flow wells surrounding the detection well 341 include flow well 342 and flow well 343, which have different structures, but in some other embodiments of the present disclosure, the flow wells of the detection well 341 may adopt the same structure, for example, all may be flow wells 342 or all may be flow wells 343, and the embodiments of the present disclosure are not limited thereto.

[0156] 11 to 13C, the structure of the directing wells is described using an example in which directing wells are opened around diamond-shaped detection wells. In other embodiments of the present disclosure, directing wells may be opened around the corresponding detection wells even when the detection wells have other regular or irregular shapes, such as triangular or circular shapes. For example, directing wells are opened around the detection wells 141 of the detection chip 10 shown in FIGS. 1A to 2B, but the embodiments of the present disclosure are not limited thereto.

[0157] For example, in an embodiment of the present disclosure, the flow wells 342, 343 surround the detection well 341, and the contour shape of the flow wells 342, 343 is the same as the contour shape of the detection well 341; in some other embodiments of the present disclosure, the contour shape of the flow wells 342, 343 may be different from the contour shape of the detection well 341, and the embodiments of the present disclosure are not limited in this regard.

[0158] For example, in some other embodiments of the present disclosure, the flow wells may partially surround the detection well, and in the case of a diamond-shaped detection well, the flow wells may be provided corresponding to only two sides of the detection well adjacent to the flow groove, thereby allowing the sample and the reaction film to mix more quickly. Also, the detection result (e.g., color development) in a certain region or range closer to the center of the reaction film becomes clearer, making it easier to observe the subsequent detection result and improving the accuracy of the obtained detection data.

[0159] For example, as shown in Figures 12 and 13A, the flow channel 330 extends to the detection well 341, and the second end of the flow channel 330 that communicates with the detection well 341 is located within the diamond-shaped detection well 341, which allows the sample to wet the reaction film 350 more quickly and thoroughly, thereby shortening the required detection time.

[0160] For example, the length of the second end of the flow guiding channel 330 extending into the detection well 341 is 1.5 mm or less, such as 0.5 mm or 1 mm, which is advantageous for guiding the sample from the flow guiding channel 330 to the detection well 341, allowing the sample to fully impregnate the reaction film 350 and fully react with the detection reagent in the reaction film 350, thereby improving the accuracy of the obtained detection data.

[0161] For example, in some embodiments of the present disclosure, the reaction film 350 is compressed along its thickness direction (i.e., the direction of height H1 shown in FIG. 13A or 13B ). For example, the thickness of the reaction film 350 in its relaxed state may be equal to or slightly greater than the height H1 of the detection well 341, and the ratio of the thickness of the reaction film 350 in its relaxed state to the height H1 of the detection well 341 may be 1:1 to 1:0.5. Furthermore, when the first substrate 301 and the second substrate 302 are bonded together, the reaction film 350 is compressed along its thickness direction, which brings the reaction film 350 and the second substrate 302 into close contact with each other, thereby preventing a gap between the reaction film 350 and the first substrate 301 or the second substrate 302 from forming and accumulating liquid.

[0162] For example, as shown in Figures 11 and 12, the detection section 340 may further include a liquid storage through-hole 380 that penetrates the first substrate 301 and communicates with the detection well 341. In this way, if an excessive amount of sample is injected through the sample supply port 310, the excess sample is stored in the liquid storage through-hole 380, thereby avoiding or reducing liquid leakage due to the injection of an excessive amount of sample. The liquid storage through-hole 380 also promotes the sample to pass through the flow-directing wells 342, 343 and wet from the periphery to the center of the reaction film 350, making the detection result (e.g., color development) at the center of the reaction film 350 clearer.

[0163] For example, the diameter of the liquid storage through-hole 380 may be 0.2 mm to 5 mm, such as 2 mm or 3 mm, which is advantageous for controlling the amount of sample, avoids or reduces leakage due to excessive injection of sample, promotes the sample wetting from the periphery to the center of the reaction film 350, reduces the requirements for the processing process and accuracy of the liquid storage through-hole 380, and simplifies the manufacturing process.

[0164] For example, as shown in Figures 13B and 13C, the ratio of the depth H3 of the liquid storage through-hole 380 to the height H1 of the detection well 341 may be 0.5:1 to 10:1, such as 3:1, which serves to quantify the sample and is advantageous for controlling the sample amount, while allowing the liquid storage through-hole 380 to store more sample and prevent sample spillage.

[0165] For example, the liquid storage through-hole 380 is connected to the center of the detection well 341, i.e., the liquid storage through-hole 380 is opened at the center position of the bottom surface of the detection well 341, connecting the center of the detection well 341 to the external environment, e.g., the atmosphere, and more effectively promoting the sample to wet from the periphery to the center of the reaction film 350.

[0166] For example, in manufacturing the detection chip 30, in order to easily assemble the detection chip 30, negative pressure can be applied to the liquid storage through-hole 380 during assembly to fix the reaction film 350 to the detection well 341. This allows the sample to react more thoroughly with the detection reagent in the reaction film 350, and further improves the accuracy of the obtained detection data.

[0167] For example, in one example of the present disclosure, the shape of the detection well 341 is diamond-shaped, and the two diagonals of the diamond shape are 8 mm and 5.8 mm, respectively, the height H1 of the detection well 341 is 0.5 mm, the difference between the height H2 of the flow guiding well 342 and the height H1 of the detection well 341 is 0.3 mm, the difference between the maximum height H4 of the flow guiding well 343 and the height H1 of the detection well 341 is 0.3 mm, the width D1 of the flow guiding well 342 is 0.3 mm, the width D2 of the flow guiding well 343 is 0.3 mm, the diameter of the liquid storage through-hole 380 is 2.5 mm, and the depth H3 of the liquid storage through-hole 380 is 1 mm. This is advantageous for controlling the amount of sample in the detection well 341, and prevents or reduces leakage due to the injection of an excessive amount of sample. It also promotes wetting of the sample from the periphery to the center of the reaction film 350, increasing the amount of sample that is wetted to the center of the reaction film 350, making the detection result (e.g., color development) at the center of the reaction film 350 clearer, which is advantageous for observing the subsequent detection results.

[0168] In some other embodiments of the present disclosure, the liquid storage through-hole may be opened at another position on the bottom surface of the detection well, or multiple liquid storage through-holes may be opened in each detection unit as needed. The embodiments of the present disclosure do not limit the arrangement position or number of liquid storage through-holes.

[0169] 14 is an exploded view, i.e., an exploded view, of a further detection chip provided in at least one embodiment of the present disclosure. FIG. 14 is a top view of the detection chip showing the structure of the detection chip as seen from the front. Note that, except for the adhesive layer 704 and the sample supply protrusion 790, the detection chip 70 shown in FIG. 14 is substantially the same as or similar in structure and function to the detection chip 30 shown in FIG. 11, and will not be described in detail here.

[0170] For example, FIG. 14 shows a detection chip 70 in which a first substrate 701 and a second substrate 702 are bonded together by an adhesive layer 704 .

[0171] For example, as shown in FIG. 14, the adhesive layer 704 is laminated between the first substrate 701 and the second substrate 702, that is, laminated on the first surface of the first substrate 701.

[0172] For example, the adhesive layer 704 may be made of a hydrophobic viscous material such as double-sided tape.

[0173] For example, as shown in FIG. 14, the adhesive layer 704 includes openings 7041 corresponding to the detection wells and flow-direction wells (not shown) of the first substrate 701, thereby avoiding the flow of the sample being hindered by the unevenness of the surface of the adhesive layer 704 or its own adhesiveness, while also avoiding the occurrence of adhesion between the adhesive layer 704 and the reaction film 750, contributing to the flow of the sample in the detection wells and flow-direction wells, allowing the sample to wet the reaction film 750 quickly and sufficiently, and improving the accuracy of the detection results obtained.

[0174] For example, in some embodiments of the present disclosure, the shape and size of the opening 7041 may be the same as the shape and size of the cross-section of the detection well, thereby reducing or avoiding interference of the adhesive layer 704 with the sample flow or reducing or avoiding adhesion between the adhesive layer 704 and the reaction film 750. In some embodiments of the present disclosure, the shape of the opening 7041 may be the same as the cross-section of the detection well, but the size of the opening 7041 may be slightly smaller than the cross-section of the detection well, thereby slightly increasing the contact area between the adhesive layer 704 and the first substrate 701 and the second substrate 702, thereby enhancing adhesion between the first substrate 701 and the second substrate 702, further reducing or avoiding sample leakage, and improving the sealing effect of the detection chip 70.

[0175] For example, the tip portion (e.g., apex portion) of the opening 7041 may be a right angle or a predetermined rounded corner, and may be, for example, a right-angled or arc-shaped. For example, taking the diamond-shaped opening 7041 in Fig. 14 as an example, the four corners of the diamond shape may be right angles or may be predetermined rounded corners, and the embodiments of the present disclosure are not limited thereto.

[0176] 14 , the two sets of diagonal angles of the diamond shape may be divided into a first set and a second set, with the diagonal angles of the first set being right angles and the diagonal angles of the second set being arc-shaped. For example, since the shape of the diamond-shaped opening 7041 is substantially the same as the outline shape of the detection well of the first substrate 701, by making the diagonal angles of the first set arc-shaped, the size of the diamond-shaped opening 7041 is slightly smaller than the cross-sectional size of the detection well, thereby increasing the contact area between the adhesive layer 704 and the first substrate 701 and the second substrate 702 to a certain extent and improving the adhesion effect between the first substrate 701 and the second substrate 702. Meanwhile, by making the diagonal angles of the second set right angles, the interference of the adhesive layer 704 with the flow of the sample to a certain extent is reduced or avoided, and the occurrence of adhesion between the adhesive layer 704 and the reaction film 750 is reduced or avoided.

[0177] As a result, by setting the above-mentioned first set of diagonals and second set of diagonals, problems such as the adhesive layer 704 interfering with the flow of the sample due to its own adhesiveness or adhesion occurring between the adhesive layer 704 and the reaction film 750 can be mitigated or avoided, and the bonding between the first substrate 701 and the second substrate 702 can be better achieved, sample leakage can be reduced or avoided, and the sealing effect of the detection chip 70 can be improved.

[0178] It should be noted that in some other examples, all of the apex angles of the opening 7041 may be right angles, or all may be arc-shaped or have other suitable contours, and the embodiments of the present disclosure are not limited in this regard.

[0179] It should be noted that in the embodiment shown in FIG. 14, the shape and size of the opening 7041 are approximately the same as the contour shape and size of the detection well and the flow well of the first substrate 701, while in some other embodiments of the present disclosure, the size of the opening in the adhesive layer may be slightly larger or smaller than the size of the detection well or the flow well, or may be of any other suitable shape or size, as long as it can reduce or avoid the adhesive layer interfering with the wetting of the reaction film by the sample and ensure the sealing effect of the detection chip, and the embodiments of the present disclosure are not limited in this regard.

[0180] For example, as shown in FIG. 14, compared with the detection chip 30 shown in FIG. 11, the detection chip 70 adopts a sample supply convex portion 790 having a petal-shaped cross section, which increases the contact area between the sample supply convex portion 790 and the first substrate 701, contributing to a stable connection between the sample supply convex portion 790 and the first substrate 701, and also making it more suitable for a user to hold the device in one hand and use it to fix or pick up.

[0181] 14, the first substrate 701 includes a first depression 7011, and the second substrate 702 includes a second depression 7021 corresponding to the first depression 7011, with the first depression 7011 and the second depression 7021 overlapping each other in a direction perpendicular to the surface of the first substrate 701 or the second substrate 702. The first depression 7011 and the second depression 7021 form a restriction portion (also called a restriction block), and the detection chip 70 is fixed to, for example, a detection device by the restriction block formed by the first depression 7011 and the second depression 7021. This further simplifies detection of the reaction film 750 on the detection chip 70, improving the accuracy of the obtained detection data. Furthermore, the positions of the first depression 7011 and the second depression 7021 allow the multiple detection branch structures of the detection chip 70 to be coded and positioned, which facilitates, for example, observation and recording of the detection data.

[0182] The sizes of the first recess 7011 and the second recess 7021 may be determined according to the structure of, for example, the detection device to which they are fixed, and the embodiments of the present disclosure are not limited in this regard, as long as the detection chip 70 can be fixed to the detection device by the first recess 7011 and the second recess 7021.

[0183] For example, in a direction perpendicular to the surface of the first substrate 701 or the second substrate 702, the first recess 7011 and the second recess 7021 may be provided between adjacent detection wells or at other suitable positions, and the embodiments of the present disclosure are not limited in this regard.

[0184] For example, if the detection chip 70 includes an adhesive layer 704 or other structural or functional layer, the structural or functional layer also has a recess structure at a position corresponding to the first recess 7011 and the second recess 7021, thereby forming a restriction block throughout the entire detection chip 70.

[0185] For example, in some embodiments of the present disclosure, structures such as a sample supply port, a flow guiding groove, a detection well, etc. of a detection chip may be provided in other forms as long as the arrangements and corresponding functions of structures such as a sample supply port, a flow guiding groove, a detection well, etc. are achieved. The above embodiments have been described as examples in which a sample supply port penetrates the first substrate and structures such as a flow guiding groove, a detection well, etc. are provided on the first surface of the first substrate of a detection chip, but this is not a limitation of the present disclosure.

[0186] Other possible arrangement positions of the flow guide grooves and detection wells will be described below by way of example based on the detection chip 30 shown in FIG.

[0187] For example, Figures 15A and 15B are exploded views, i.e., exploded views, of a further detection chip provided in at least one embodiment of the present disclosure. For example, Figure 15A is a top view of the detection chip, and Figure 15B is a bottom view of the detection chip. Note that for the detection chip 40 shown in Figures 15A and 15B, the structures and functions of specific components can be referenced to the corresponding descriptions of the detection chip 10, detection chip 20, or detection chip 30 in the above embodiments, and will not be described in detail here.

[0188] For example, as shown in FIGS. 15A and 15B, the detection chip 40 includes a first substrate 401, a second substrate 402, and a third substrate 403.

[0189] For example, the first substrate 401 has a first surface (top surface in the figure) and a second surface (bottom surface in the figure) that face each other, and the flow guiding groove 430 is formed on the second surface of the first substrate 401, and the detection well 441 is formed on the first surface of the first substrate 401. The second substrate 402 is laminated on the first surface of the first substrate 401, and has a detection through-hole 460 at a position corresponding to the detection well 441 so that optical inspection can be performed. The third substrate 403 is laminated on the second surface of the first substrate 401, and seals the flow guiding groove 430 and the sample supply port 410, allowing the sample to flow from the sample supply port 410 into the flow guiding groove 430.

[0190] For example, the sample supply port 410 is a through-hole that penetrates the first substrate 401, and the second substrate 402 has a through-hole that exposes the sample supply port 410, so that the sample can be injected into the sample supply port 410, and the sample supply protrusion 490 protrudes from the second substrate 402, facilitating, for example, picking up or setting the detection chip 40.

[0191] For example, Fig. 16 is an exploded view, i.e., an exploded view, of a further detection chip provided in at least one embodiment of the present disclosure. For example, Fig. 16 is a top view of the detection chip. Note that for the detection chip 50 shown in Fig. 16, the structures and functions of specific components can refer to the corresponding descriptions of the detection chip 10, detection chip 20, or detection chip 30 in the above embodiments, and will not be described in detail here.

[0192] 16, for example, a detection chip 50 includes a first substrate 501 and a second substrate 502. The first substrate 501 has a first surface (upper surface in the figure) and a second surface (lower surface in the figure) that face each other, and a flow guide groove 530 and a detection well 541 are formed on the first surface of the first substrate 501. The second substrate 502 is stacked on the first surface of the first substrate 501 and has a detection through-hole 560 at a position corresponding to the detection well 541 so as to enable optical inspection.

[0193] For example, the sample supply port 510 is a hole in the first substrate 501, and the hole is, for example, a blind hole that does not penetrate the first substrate 501. In such a case, the height (or depth) of the sample supply port 510 is smaller than the thickness of the first substrate 501. The second substrate 502 has a through hole exposing the sample supply port 510 and a sample supply protrusion 590, which allows the sample to be injected into the sample supply port 510 through the sample supply protrusion 590 and also makes it easier to, for example, pick up or set the detection chip 50.

[0194] At least one embodiment of the present disclosure also provides a detection system, which includes a detection device and a detection chip provided in any embodiment of the present disclosure, such as detection chip 10, detection chip 20, detection chip 30, detection chip 40 or detection chip 50 in the above embodiments, and the detection device is configured to detect the reaction film in the detection well by the detection portion of the detection chip.

[0195] For example, taking the detection chip 10 in the above-described embodiment as an example, FIG. 17 is a schematic diagram of a detection system provided in at least one embodiment of the present disclosure.

[0196] For example, as shown in FIG. 17, the detection system 60 includes a detection chip 10 and a detection device 610, and the detection device 610 is configured to detect a reaction reagent, such as a reaction film 150, in a detection well 141 using the detection portion 140 of the detection chip 10.

[0197] For example, the detection device 610 includes a light source 611 and a photoelectric detection device 612. The light source 611 is configured to emit light toward the reactive film 150, and the photoelectric detection device 612 is configured to receive light emitted from the light source 611 and reflected by the reactive film 150.

[0198] For example, the photoelectric detection device 612 can detect an indicator of the sample by comparing the intensity of light reflected by the reaction film 150 with the intensity of light emitted from the light source 611 and determining the presence or absence and concentration of the analyte in the sample from the detected absorbance value of the reaction film 150. For example, taking a case where the detection reagent preliminarily titrated into the reaction film 150 is a color-developing reagent as an example, when obtaining the detection result, the darker the color that appears on the reaction film 150, the higher the content of the analyte in the sample to be detected, and correspondingly, the larger the absorbance value of the reaction film 150 detected using the photoelectric detection device 612.

[0199] For example, in some embodiments, the photoelectric detection device 612 may be a photodiode that converts the received optical signal into an electrical signal and determines the intensity of the received light from a change in an electrical parameter in the electrical signal (e.g., a change in current), thereby determining the absorbance value of the reactive film 150.

[0200] For the specific description and technical effects of the detection system provided in the embodiments of the present disclosure, reference can be made to the corresponding content of the detection chip provided in the embodiments of the present disclosure, for example, the corresponding content of detection chip 10, detection chip 20, detection chip 30, detection chip 40 or detection chip 50 in the above embodiments, and will not be described in detail here.

[0201] A few points need to be explained:

[0202] (1) The drawings of the embodiments of the present disclosure relate only to the structure of the embodiments of the present disclosure, and other structures may refer to conventional designs.

[0203] (2) For clarity, the thickness of the drawings, layers, or regions illustrating the embodiments of the present disclosure have been enlarged or reduced, i.e., the drawings are not made to actual proportions. However, when a component, such as a layer, film, region, or first substrate, is described as being "above" or "below" another component, the component may be "directly above" or "below" the other component, or intermediate components may be present.

[0204] (3) Unless inconsistent, the embodiments and features of the embodiments of the present disclosure may be combined with each other to obtain new embodiments.

[0205] The above are merely specific embodiments of the present disclosure, and the patent scope of the present disclosure is not limited thereto, and should be governed by the scope of protection of the claims.

Claims

1. A detection chip, comprising: A sample supply port; and at least one detection branch structure, each of the at least one detection branch structure comprising: a detection well in communication with the sample supply port; a reaction reagent contained in the detection well; a detection unit configured to allow optical inspection of the reaction reagent in the detection well; Each of the at least one detection branch structure comprises: The detection chip further includes a water-absorbent membrane contained within the detection well, at least partially overlapping the reaction reagent in the thickness direction of the water-absorbent membrane, and positioned in contact with the reaction reagent, the reaction reagent being located outside the water-absorbent membrane.

2. each of the at least one detection branch structure further includes a flow guide groove; The detection chip of claim 1, wherein the flow guide groove has a first end communicating with the sample supply port and a second end communicating with the detection well, and the detection well communicates with the sample supply port via the flow guide groove.

3. a first substrate having a first surface, the sample supply port being a through-hole in the first substrate, and the flow guide channel and the detection well being formed on the first surface of the first substrate; The detection chip according to claim 2 , further comprising a second substrate laminated on the first surface of the first substrate and enabling the optical inspection at a position corresponding to the detection well.

4. the detection unit further includes a flow guide well located around the detection well and communicating with the detection well; The sensing chip of claim 2 , wherein the height of at least a portion of the flow well is smaller than the height of the sensing well.

5. The flow well 5. The detection chip according to claim 4, wherein one end of the detection chip is connected to a side surface of the detection well and includes an inclined flow guide wall.

6. The detection chip according to claim 4 , wherein the longitudinal cross sections of the flow guide well and the detection well are stepped as a whole.

7. 7. The detection chip according to claim 4, wherein the flow guide well at least partially surrounds the detection well.

8. The detection chip according to claim 3 , wherein the detection unit further includes a liquid storage through-hole that penetrates the first substrate and communicates with the detection well.

9. The at least one detection branch structure 9. The detection chip according to claim 2, comprising a plurality of detection branch structures evenly arranged around the sample supply port.

10. 4. The detection chip according to claim 3, wherein the sample supply port includes a first body and a first protrusion that communicates with the flow guiding groove and protrudes from the first body into the flow guiding groove.

11. The detection chip according to any one of claims 2 to 10, wherein the reaction reagent comprises a reaction film and / or a bulk reaction reagent.

12. the reaction film is circular, or The detection chip according to claim 11 , wherein the reaction film is polygonal, and one corner of the polygon is directly connected to the second end of the flow guide channel.

13. the reaction film includes a film body and a second protrusion, at least a portion of which is located within the flow guiding groove and which protrudes from the film body into the flow guiding groove; The sensing chip of claim 11 , wherein the film body is circular in shape, as are the sensing wells.

14. The detection chip according to claim 3 or 8, wherein the second substrate has detection through-holes at positions corresponding to the detection wells.

15. the second substrate is non-transparent, or The detection chip according to claim 14 , further comprising a light-shielding layer covering a surface of the second substrate that is distant from and / or close to the first substrate and that exposes the detection through-hole.

16. a first substrate having a first surface, the sample supply port being a through-hole in the first substrate, and the flow guide channel and the detection well being formed on the first surface of the first substrate; a second substrate laminated on the first surface of the first substrate, having a detection through-hole at a position corresponding to the detection well, and enabling the optical inspection through the detection through-hole; The detection chip according to claim 2 , wherein the at least one detection branch structure includes a plurality of detection branch structures, the plurality of detection branch structures being evenly arranged around the sample supply port.

17. a first substrate having a first surface and a second surface facing each other, the sample supply port being a through-hole in the first substrate, the detection well being formed in the first surface of the first substrate, and the flow guide groove being formed in the second surface of the first substrate; a second substrate laminated on the first surface of the first substrate and enabling the optical inspection at a position corresponding to the detection well; 3. The detection chip according to claim 2, further comprising a third substrate laminated on the second surface of the first substrate and sealing the sample supply port and the flow guide channel.

18. a first substrate having a first surface, the sample supply port, the flow guide groove, and the detection well being formed on the first surface of the first substrate, and the sample supply port being a blind hole in the first substrate; The detection chip of claim 2 , further comprising a second substrate laminated to the first surface of the first substrate, exposing the sample supply port and enabling the optical inspection at a position corresponding to the detection well.

19. The detection chip according to claim 3 , wherein the water-absorbent film is provided on a side of the reaction reagent that is remote from the second substrate.

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