A method for reducing amylose content in rice and regulating the expression of Wx genes in rice

Abstract

The present invention discloses a method for reducing the amylose content of rice and regulating the expression of Wx gene in rice, which belongs to the field of agricultural production. The method involves irrigating a rice field with ordinary hydrogen-rich water and / or nanobubble hydrogen water by flood irrigation and / or sprinkler irrigation from the rooting stage to the ripening stage, thereby efficiently reducing the amylose content of rice. The method described in the present invention can improve the quality of rice and enhance the eating quality.

Description

[Technical Field]

[0001] The present invention relates to a method for reducing amylose content in rice and regulating Wx gene expression in rice, particularly to the application of hydrogen-enriched water to reducing amylose content in rice and regulating Wx gene expression in rice. [Background technology]

[0002] Paddy rice is an important and widely cultivated grain crop. Asia accounts for around 90% of the world's paddy rice production. After undergoing processes such as sorting, hulling, milling, and product sorting, the resulting product (also known as "rice" or "rice rice") is an important staple food. With economic development and continuous improvements in living standards, people's concerns about rice quality are also increasing. For this reason, people are constantly improving agricultural production techniques to improve the nutritional and eating quality of rice.

[0003] Starch in rice is a polysaccharide polymer composed of glucose, containing amylopectin, which is primarily branched, and amylose, which is primarily linear. The content, molecular weight, spatial structure, and interrelationship between these two types of starch are important factors that affect rice quality and can directly affect the water absorption and volume expansion of rice during the steaming process. If the amylose content is high, cooked rice after steaming will have a weak chewiness, low viscosity, low elasticity, and dull luster. Conversely, if the amylose content is low, cooked rice after steaming will have a relatively high viscosity, strong chewiness, high elasticity, and a good texture. Generally, rice with an amylose content of 20% or more has poor taste, while rice with an amylose content of 15% to 20% or less has good taste.

[0004] One of the main reasons for the slow progress in rice quality improvement is the complexity of rice quality genetics and the limitations of traditional breeding methods. A Chinese invention patent with patent number CN105671183B discloses the molecular tagging and application of the rice amylose content fine-regulatory gene AGPL3. The patent notes that traditional breeding methods primarily involve directed selection and fixation of advantageous target traits to develop superior new varieties, but this involves significant blindness and unpredictability. Therefore, the selective breeding process for low-amylose rice varieties is complex, costly, and has low genetic stability, resulting in often unsatisfactory selective breeding results. Large-scale environmental release and commercial production of genetically modified rice may also pose potential biological safety issues. Therefore, improving rice quality through improved agricultural production techniques has become a new trend.

[0005] Hydrogen gas (H2) is a common, reducing gas. Chinese invention patent No. CN102657221B discloses a hydrogen-rich liquid plant growth regulator and its preparation and application methods. The patent states that the hydrogen-rich liquid plant growth regulator can enhance crop stress resistance, stress tolerance, agronomic traits, and metabolic function, and has cost-effectiveness and environmental advantages over other chemical plant growth regulators. Hydrogen gas acts as a signaling molecule, enhancing crops' ability to resist heavy metals and UV radiation. Hydrogen gas can also promote crop nutrient absorption and improve produce quality. However, hydrogen gas is expensive to prepare, has a short half-life, and exhibits limited efficacy.

[0006] A Chinese invention patent with patent number CN106699330A discloses the preparation and application of slow-release / controlled-release hydrogen fertilizer or compound hydrogen fertilizer. The patent discloses the application of magnesium dihydride (MgH2) and other compounds as slow-release fertilizers to agricultural land to promote crop growth. However, the application of magnesium dihydride to soil produces alkaline substances, which can easily lead to serious localized salinization of the soil. Summary of the Invention [Problem to be solved by the invention]

[0007] This application discloses a method for reducing the amylose content of rice and a method for regulating the expression of the Wx gene in rice. Unlike the prior art method of improving rice quality through rice breeding, this application uses hydrogen-rich water to irrigate rice paddies. Furthermore, this application employs a micro-nano aeration device to prepare nanobubble hydrogen water with high hydrogen gas concentration, a long half-life, and low cost, resulting in more pronounced biological effects.

[0008] A first aspect of the present invention provides a method for reducing the amylose content of paddy rice, which comprises irrigating a paddy rice field with ordinary hydrogen-enriched water and / or nanobubble hydrogen water.

[0009] Furthermore, the concentration of dissolved hydrogen in the normal hydrogen-rich water and / or nanobubble hydrogen water has a specific concentration range, more preferably 500 to 1600 ppb, even more preferably 700 to 1400 ppb, and most preferably 1000 to 1200 ppb.

[0010] Furthermore, irrigation with normal hydrogen-rich water and / or nanobubble hydrogen water reduces the amylose content by 4% to 40%.

[0011] Furthermore, from the time of rice planting to the time of ripening, the total amount of irrigation water used is 1200 to 5400 m 3 / hm 2 is.

[0012] Furthermore, in each season, from the rice rooting stage to the ripening stage, irrigation using ordinary hydrogen-rich water and / or nanobubble hydrogen water is carried out 3 to 8 times.

[0013] Furthermore, in the nanobubble hydrogen water, the diameter of the nanobubbles is in the range of 30 to 600 nm, and more preferably in the range of 30 to 500 nm.

[0014] Furthermore, the half-life of the hydrogen gas in the nanobubble hydrogen water is 3 to 8 hours.

[0015] Furthermore, rice is irrigated by flood irrigation and / or sprinkler irrigation methods.

[0016] Furthermore, the nanobubble hydrogen water can be obtained by mixing hydrogen gas with an irrigation water source using a micro-nano aeration device.

[0017] A second aspect of the present invention provides a method for regulating the expression of the Wx gene in rice by irrigating a rice paddy with ordinary hydrogen-rich water and / or nanobubble hydrogen water. Specifically, irrigating a rice paddy with ordinary hydrogen-rich water and / or nanobubble hydrogen water reduces the expression level of the Wx gene in rice, thereby reducing the amylose content in the rice.

[0018] Therefore, the method for reducing the amylose content in rice provided by the first aspect of the present invention can reduce the amylose content in rice by regulating the expression of the Wx gene.

[0019] The technical solution of the present application not only provides a practical basis for the research of the developmental physiology of agricultural crops, but also provides a new practical concept for the chemical regulation and development of agricultural products, and also has the following advantages:

[0020] 1. The applicant has found that irrigation with normal hydrogen-rich water and / or nanobubble hydrogen water at a specific concentration (less than the saturated concentration) can effectively reduce the amylose content of rice and improve the nutritional and eating quality of the rice. Although the range of the specific concentration varies to some extent for different rice varieties, the applicant still observed this remarkable characteristic when exerting the biological effects of normal hydrogen-rich water and / or nanobubble hydrogen water.

[0021] 2. The applicant found that the hydrogen gas in nanobubble hydrogen water is dissolved as much as possible, has a longer residence time in water, and a longer half-life, which results in a more significant reduction in the amylose content of rice after irrigation with nanobubble hydrogen water, which is more suitable for the actual situation of large irrigation areas and long irrigation times in agricultural production.

[0022] 3. Ordinary hydrogen-rich water and / or nanobubble hydrogen water can be used as irrigation water directly and are not irritating to the human body. They disperse quickly after irrigating agricultural land. Their chemical properties are stable and highly safe, and their hydrogen content is far below the minimum explosive level (approximately 4%).

[0023] 4. Ordinary hydrogen-rich water and / or nanobubble hydrogen water are composed only of hydrogen gas and water, are non-contaminated, and pose no risk of adverse effects on the human body or the environment. DETAILED DESCRIPTION OF THE INVENTION

[0024] Specific examples of the present application will be described in detail below. However, it should be understood that the present application is not limited to the embodiments described below, and the technical ideas of the present application can be implemented in combination with other known technologies or functions, or other technologies that are the same as these known technologies.

[0025] The terms "first" and "second" are used for descriptive purposes only and do not imply any limitation on the chronological order, quantity, or importance, nor should they be understood as indicating or implying the relative importance or implicitly referring to the number of technical features indicated. They are merely used to distinguish one technical feature from another in the technical solution. Accordingly, a feature qualified as "first" or "second" may explicitly or implicitly include one or more of the feature. In this description, unless otherwise clearly and specifically specified, "plurality" means two or more than two. Similarly, limiting words similar to "one" appearing in this specification do not imply any limitation on quantity, but rather describe a technical feature not previously mentioned. Similarly, unless a noun is modified by a specific quantitative classifier, the specification should be deemed to include both the singular and the plural, and the technical solution may include either a single or multiple of the technical feature. Similarly, modifiers such as "about," "approximately," and the like that appear before numerals in this specification are generally inclusive of the numeral, and their specific meaning must be understood in conjunction with the context.

[0026] In this application, it should be understood that "at least one" refers to one or more, and "multiple" refers to two or more. "And / or" is used to describe a relationship between related objects and indicates that three types of relationships may exist. For example, "A and / or B" can represent three situations: only A exists, only B exists, or both A and B exist, where A and B may be singular or plural. The symbol " / " generally indicates that the related objects before and after it are in a type of "or" relationship. "At least one of the following" or similar expressions refers to any combination of those items, including any combination of a singular item or multiple items. For example, "at least one of a, b, or c" can represent a, b, c, "a and b," "a and c," "b and c," or "a and b and c," where a, b, and c may be singular or plural.

[0027] Unless expressly stated to the contrary, each aspect or embodiment defined herein may be combined with any other aspect or embodiment. In particular, any feature that is stated to be preferred or advantageous may be combined with any other feature that is stated to be preferred or advantageous.

[0028] Gas-liquid mixture fluids containing microbubbles (i.e., millimeter-, micrometer-, and nanometer-sized bubbles) are used in a variety of industries. The micro-nano aeration device described herein dissolves gas into liquids using a high-speed swirling cutting method, generating micro-nano bubbles with diameters of less than 600 nm, thereby rapidly and efficiently dissolving the gas into the liquid. The micro-nano aeration device can improve gas dissolution efficiency. Micro-nano bubbles have advantages such as small size, large specific surface area, slow rising speed, and long residence time. Research has shown that the slower the decay rate of micro-nano bubbles, the more adaptable they are to aeration needs. The micro-nano aeration device used in this application may be a device employing micro-nano bubble technology familiar to those skilled in the art, and mainly comprises a pipeline, a micro-nano gas-liquid mixing tank, a micro-nano aeration head, a control system, and an interlock system. The rated water production range of the device is approximately 25 to 40 tons / hour, and the hydrogen gas inlet flow rate can reach 30 to 50 SLMP. The nanobubble hydrogen water produced by this device has an average bubble diameter ranging from 30 to 500 nm, and even ranging from 250 to 350 nm, and can even reach an average diameter of approximately 300 nm. The dissolved hydrogen concentration of the nanobubble hydrogen water produced by this device can be adjusted as needed within the range of 0 to 1600 ppb.

[0029] As used herein, "half-life" refers to the time required for the concentration to decrease by half. After hydrogen gas dissolves in water, it slowly separates from the water even in an open container, and the hydrogen gas concentration in the water gradually decreases. This is called the "dissolution" phenomenon. In an open container, the half-life of hydrogen gas in a normal container is approximately 1 to 2 hours, while the half-life of hydrogen gas in nanobubble hydrogen water is approximately 3 to 8 hours, depending on the concentration.

[0030] As used herein, "hydrogen-rich water" (HRW) refers to water rich in hydrogen gas. The solubility of hydrogen gas refers to the number of volumes of hydrogen gas (at 1 standard atmosphere) that dissolve in one volume of water at a certain temperature. Under standard conditions (1 atmosphere, 20°C), the solubility of hydrogen gas is 1.83%, meaning that 1.83 milliliters of hydrogen gas can be dissolved in 100 milliliters of water. The above volume and mass ratios can be converted; 1.6 mg of hydrogen gas dissolved in 1 liter of water means that the saturated concentration of hydrogen water is 1.6 ppm.

[0031] Ordinary hydrogen-rich water: A hydrogen gas generator (model SHC-500, Sikescis, Shandong, China) electrolyzes potassium hydroxide solution using direct current, separating the water and gas, and then hydrogen gas (purity > 99.999%) is obtained. This hydrogen gas is then passed through the irrigation water source for a certain period of time to produce ordinary hydrogen-rich water with hydrogen gas bubbles of 1 μm or more in diameter. Ordinary hydrogen-rich water can be used alone or mixed with the irrigation water source to achieve the desired concentration.

[0032] Nanobubble hydrogen water: Nanobubble hydrogen water is prepared by mixing hydrogen gas with an irrigation water source using a micro-nano aeration device. In nanobubble hydrogen water, ultrafine bubbles encapsulate the hydrogen gas, preventing it from escaping. This application allows the nanobubble hydrogen water to be prepared to the required concentration, either alone or by mixing with an irrigation water source, depending on the needs of agricultural irrigation. The hydrogen gas may be derived from cylinder gas or may be hydrogen gas prepared by physical / chemical methods. As used herein, "nanobubble hydrogen water" can be understood to mean hydrogen-rich water containing nanobubbles with diameters ranging from 30 nm to 600 nm. The nanobubbles may have an average diameter of less than 600 nm, or less than 500 nm, or in the range of about 30 to 400 nm, or in the range of about 50 to 350 nm, or in the range of about 75 to 300 nm, or in the range of about 100 to 250 nm, or in the range of about 100 to 200 nm. The dissolved hydrogen concentration of the nanobubble hydrogen water may reach 200 to 1600 ppb, more preferably 300 to 1500 ppb, even more preferably 600 to 1400 ppb, and most preferably 800 to 1300 ppb. In some embodiments, these nanobubbles are stable in the liquid carrier for at least about 15 hours under ambient pressure and temperature.

[0033] As used herein, the "dissolved hydrogen concentration" in ordinary hydrogen-rich water and / or nanobubble hydrogen water can be understood to mean the "outlet hydrogen concentration," which refers to the dissolved hydrogen concentration measured at the outlet of a hydrogen gas generator or micro-nano aeration device. Taking into account the dissipation of hydrogen gas, those skilled in the art know that the concentration of ordinary hydrogen-rich water and / or nanobubble hydrogen water used for irrigating agricultural land can be brought as close as possible to the outlet hydrogen concentration by, for example, continuously adding hydrogen water, such as an outlet hydrogen concentration of 80% or more, more preferably an outlet hydrogen concentration of 85% or more, even more preferably an outlet hydrogen concentration of 90% or more, and most preferably an outlet hydrogen concentration of 95% to 99.9%.

[0034] As used herein, the term "agricultural land" refers to land used for agricultural production, cultivated fields, and includes, but is not limited to, land or fields used to cultivate food crops, economic crops (oil crops, vegetable crops, flowers, pasture, and fruit trees), industrial crops, fodder crops, and herbal medicines. Preferably, the term refers to land used to cultivate plants that can be grown in large quantities or harvested over large areas and are used for profit or food (e.g., grains, vegetables, cotton, flax, etc.). More preferably, the term refers to land or fields used to cultivate rice, corn, beans, potatoes, naked barley, broad beans, wheat, rapeseed, turnip, bamboo shoots, peanuts, sesame, hemp, sunflower, radish, Chinese cabbage, spinach, garlic, green onions, carrots, Chinese yam, cabbage, Jerusalem artichoke, sword beans, coriander, stem lettuce, golden needle lettuce, chili peppers, cucumbers, tomatoes, coriander, etc. In this application, the term "field" is equivalent to "farmland" and there are no special requirements regarding the area or shape of the field or farmland.

[0035] For the measurement of amylose content, we referred to the provisions in GB / T1354-2018, which states that the amylose content in a sample is the percentage of the total mass of the sample.

[0036] Research has shown that amylose synthesis in endosperm is catalyzed by the granule-binding enzyme (GBSS1) encoded by the Wx gene. In recent years, breeders have conducted extensive genetic research on the relationship of the Wx gene to rice quality and traits, and have identified the different effects of various Wx alleles on rice amylose content for further use in improving rice quality and developing new varieties. The ordinary hydrogen-enriched water and / or nanobubble hydrogen water used in this application can regulate the expression levels of rice Wx genes and their alleles. Research has shown that amylose synthesis in endosperm is catalyzed by the starch synthase encoded by the Wx gene on starch granules.

[0037] The gene expression level of the Wx allele was measured by Livak, KJ et al. Analysis of relative gene expression data using real-time quantitative PCR and the 2- △△CT The method was performed with reference to the method described in Methods. Methods 2001, 25, 402-408. An internal standard gene was used as a reference, and the expression level of the internal standard gene can accurately quantify the amount of initial material loaded. An internal standard gene refers to a known standard gene whose expression level is not affected by research conditions and can be consistently expressed among multiple samples.

[0038] The growth stages of paddy rice generally require the following stages: soaking seeds, germination, sowing, seedling stage, transplanting, rooting stage, tillering stage, young rice differentiation stage, heading and flowering stage, ripening stage, and maturity stage.

[0039] In the technical solution of the present application, nanobubble hydrogen water and / or ordinary hydrogen-enriched water are irrigated 3 to 8 times from the rooting stage to the ripening stage by flood irrigation and / or sprinkler irrigation. The total amount of nanobubble hydrogen water and / or ordinary hydrogen-enriched water used for rice plants in each season is approximately 1,200 to 5,400 m 3 / hm 2 In a preferred embodiment, nanobubble hydrogen water and / or normal hydrogen-enriched water is irrigated during the rooting, tillering, heading, flowering, and ripening stages. The amount of irrigation water used each time depends on the local climate, surface water standards, soil quality, rice variety, and rainfall. It should be noted that field agriculture refers to the cultivation of crops in large fields. The main difference from laboratory cultivation is that the environment of a field is complex, has low controllability, and is not easy to manage with high precision. In this application, the appropriate amount of irrigation water is to flood the soil to a water level of approximately 2 to 10 cm above the soil surface. The specific amount of irrigation water often needs to be adjusted based on agricultural practice and experience.

[0040] The outlet hydrogen concentration is measured using a dissolved hydrogen meter ENH-2000 (TRUSTLEX, Japan; correction is also performed by gas chromatography).

[0041] The term "rice" refers to the product obtained by processing plant seeds belonging to the species Oryza sativa. [Example]

[0042] Example 1: Effect of irrigation with normal hydrogen-rich water and nanobubble hydrogen water on the amylose content of rice variety "Nan-nyu 5055" In this example, the test subject was the paddy rice variety "Nan-Nitsu 5055." A split-plot method was used for the paddy rice fields, with three identical plots set up for each of the following groups: Each plot had an area of ​​4m x 4m, with 40 holes, 10 seedlings per hole, and a protection row between each plot. During the cultivation period, the following different irrigation methods were used, with irrigation carried out six times from the rooting stage to the ripening stage, with irrigation water volume of 450m each. 3 / hm 2 The total amount of irrigation water is 2700m 3 / hm 2 Farmland management is carried out in accordance with local pest and disease control methods. After the rice is harvested, the amylose content in the rice is measured.

[0043] The irrigation methods for each group of farmland are as follows: Group 1-1: Normal surface water is used as the irrigation source. Group 1-2: Normal hydrogen-rich water with an outlet hydrogen concentration of 200-300 ppb was used as the irrigation water source. Group 1-3: Normal hydrogen-rich water with an outlet hydrogen concentration of 500-700 ppb was used as the irrigation water source. Group 1-4: Normal hydrogen-rich water with an outlet hydrogen concentration of 1000-1200 ppb was used as the irrigation water source. Group 1-5: Normal hydrogen-rich water with an outlet hydrogen concentration of 1400-1600 ppb was used as the irrigation water source. Groups 1-6: Nanobubble hydrogen water with an outlet hydrogen concentration of 200-300 ppb was used as the irrigation water source. Groups 1-7: Nanobubble hydrogen water with an outlet hydrogen concentration of 500-700 ppb was used as the irrigation water source. Groups 1-8: Nanobubble hydrogen water with an outlet hydrogen concentration of 1000-1200 ppb was used as the irrigation water source. Groups 1-9: Nanobubble hydrogen water with an outlet hydrogen concentration of 1400-1600 ppb was used as the irrigation water source.

[0044] The experimental results are shown in Table 1.

[0045] [Table 1]

[0046] As shown in Table 1, the amylose content of the "Nan-Nitsu 5055" rice variety was reduced to some extent after irrigation with either normal hydrogen-enriched water or nanobubble hydrogen water compared to surface water irrigation. The effect of nanobubble hydrogen water irrigation on reducing amylose content was even more pronounced. Taking normal hydrogen-enriched water and nanobubble hydrogen water with concentrations of 1000-1200 ppb as examples, the amylose content of rice irrigated with nanobubble hydrogen water was reduced by a further 8.3%. Furthermore, the amylose content of rice irrigated with nanobubble hydrogen water was lower than that of rice irrigated with normal hydrogen-enriched water at the same hydrogen gas concentration.

[0047] The present applicant further unexpectedly discovered that the higher the concentration of ordinary hydrogen-rich water or nanobubble hydrogen water, the greater the effect of reducing amylose content. Table 1 shows that the amylose content of ordinary hydrogen-rich water or nanobubble hydrogen water was most significantly reduced at concentrations of 1000-1200 ppb, with reductions of approximately 19% and 27.3%, respectively, compared to Group 1-1. As the concentration of ordinary hydrogen-rich water or nanobubble hydrogen water increased to the 1400-1600 ppb range, the effect of reducing amylose content did not become even more pronounced.

[0048] Example 2: Effect of irrigation with normal hydrogen-rich water and nanobubble hydrogen water on the amylose content of rice variety "Kanzabanten 35" In this example, the rice variety "Kanz Bansen 35" is used as the test subject. A split-plot method is used for the rice paddies, with three identical plots set up for each of the following groups: Each plot has an area of ​​4m x 4m, with 40 holes, 10 seedlings per hole, and a protection row is also set up between each plot. During the cultivation period, the following different irrigation methods are used, with irrigation carried out eight times from the rooting stage to the ripening stage, with irrigation water volume of 600m per time. 3 / hm 2 The total amount of irrigation water is 4,800 m 3 / hm 2 Farmland management is carried out in accordance with local pest and disease control methods. After the rice is harvested, the amylose content in the rice is measured.

[0049] The irrigation methods for each group of farmland are as follows: Group 2-1: Normal surface water is used as the irrigation source. Group 2-2: Normal hydrogen-rich water with an outlet hydrogen concentration of 200-300 ppb was used as the irrigation water source. Group 2-3: Normal hydrogen-rich water with an outlet hydrogen concentration of 500-700 ppb was used as the irrigation water source. Groups 2-4: Normal hydrogen-rich water with an outlet hydrogen concentration of 1000-1200 ppb was used as the irrigation water source. Groups 2-5: Normal hydrogen-rich water with an outlet hydrogen concentration of 1400-1600 ppb was used as the irrigation water source. Groups 2-6: Nanobubble hydrogen water with an outlet hydrogen concentration of 200-300 ppb was used as the irrigation water source. Groups 2-7: Nanobubble hydrogen water with an outlet hydrogen concentration of 500-700 ppb was used as the irrigation water source. Groups 2-8: Nanobubble hydrogen water with an outlet hydrogen concentration of 1000-1200 ppb was used as the irrigation water source. Group 2-9: Nanobubble hydrogen water with an outlet hydrogen concentration of 1400-1600 ppb was used as the irrigation water source.

[0050] The experimental results are shown in Table 2.

[0051] [Table 2]

[0052] As shown in Table 2, the amylose content of the rice variety "Kanzabantosen 35" was reduced to some extent after irrigation with normal hydrogen-enriched water and nanobubble hydrogen water compared to surface water irrigation. The effect of nanobubble hydrogen water irrigation in reducing amylose content was even more pronounced. Taking normal hydrogen-enriched water and nanobubble hydrogen water with concentrations of 1000-1200 ppb as examples, the amylose content of rice irrigated with nanobubble hydrogen water was reduced by an additional 3.4%. Furthermore, the amylose content of rice irrigated with nanobubble hydrogen water was lower than that of rice irrigated with normal hydrogen-enriched water with the same hydrogen gas concentration.

[0053] Table 2 shows that the amylose content of both ordinary hydrogen-rich water and nanobubble hydrogen water decreased most significantly when the concentration was in the range of 1000-1200 ppb, with reductions of approximately 20.7% and 35% compared to Group 2-1. As the concentration of ordinary hydrogen-rich water or nanobubble hydrogen water increased to the range of 1400-1600 ppb, the effect of reducing amylose content did not improve.

[0054] Example 3: Effect of nanobubble hydrogen water irrigation on the amylose content of rice variety "Huran 1212" In this example, the rice variety "Huran 1212" was used as the test subject. Two test plots were set up, each with an area of ​​0.8 hm. 2A protection row is set up between the different test plots. Farmland management is carried out in accordance with the local normal methods for pest control, etc. During the cultivation period, the following different irrigation methods are adopted, and irrigation is carried out six times from the rooting stage to the ripening stage, with irrigation water volume of 300m per time. 3 / hm 2 The total amount of irrigation water is 1800m 3 / hm 2 Farmland management is carried out in accordance with local pest and disease control methods. After the rice is harvested, the amylose content in the rice is measured.

[0055] The irrigation methods for each group of farmland are as follows: 3-1: Use normal surface water as an irrigation source. 3-2: Nanobubble hydrogen water with an outlet hydrogen concentration of 1000 to 1200 ppb is used as the irrigation water source.

[0056] The experimental results are shown in Table 3.

[0057] [Table 3]

[0058] Table 3 shows that after irrigation with a specific concentration of nanobubble hydrogen water, the amylose content of "Huran 1212" rice was significantly reduced by about 18.6%. After treatment with nanobubble hydrogen water, the relative expression level of the Wx gene in "Huran 1212" rice was reduced by about 65%.

[0059] Example 4: Effect of nanobubble hydrogen water irrigation on amylose content of rice variety "Hanayu 14" In this example, the rice variety "Hanayu 14" was used as the test subject. Two test plots were set up, each with an area of ​​0.667 hm 2 A protection row is set up between the different test plots. Farmland management is carried out in accordance with the local normal methods for pest and disease control. During the cultivation period, the following different irrigation methods are adopted, and irrigation is carried out three times from the rooting stage to the ripening stage, with irrigation water volume of 375 m per time. 3 / hm2 The total amount of irrigation water is 1125m 3 / hm 2 After the rice is harvested, the amylose content in the rice is measured.

[0060] The irrigation methods for each group of farmland are as follows: 4-1: Use normal surface water as an irrigation source. 4-2: Nanobubble hydrogen water with an outlet hydrogen concentration of 1000 to 1200 ppb is used as the irrigation water source.

[0061] The experimental results are shown in Table 4.

[0062] [Table 4]

[0063] Table 4 shows that after irrigation with nanobubble hydrogen water at a specific concentration, the amylose content of the rice variety "Hanayou 14" decreased significantly by about 16.8%.

[0064] Example 5: Effect of nanobubble hydrogen water irrigation on the amylose content of paddy rice "Seika Nan-naku" In this example, the rice variety "Seika Nan Jin" was used as the test subject. Two test plots were set up, each with an area of ​​0.8 hm 2 A protection row was also provided between the different test plots. During the cultivation period, the following different irrigation methods were used, and irrigation was carried out six times from the rooting stage to the ripening stage, with the amount of irrigation water being 675 m per time. 3 / hm 2 The total amount of irrigation water is 4050m 3 / hm 2 Farmland management is carried out in accordance with local pest and disease control methods. After the rice is harvested, the amylose content in the rice is measured.

[0065] The irrigation methods for each group of farmland are as follows: 5-1: Use normal surface water as an irrigation source. 5-2: Nanobubble hydrogen water with an outlet hydrogen concentration of 1000-1200 ppb is used as the irrigation water source.

[0066] The experimental results are shown in Table 5.

[0067] [Table 5]

[0068] As shown in Table 5, after irrigation with nanobubble hydrogen water at a specific concentration, the amylose content of the rice variety "Qingxiang Soft Non-Non-GMO" decreased significantly by approximately 38.1%.

[0069] Therefore, the technical solution of this application shows that irrigation with a specific concentration of normal hydrogen-rich water and / or nanobubble hydrogen water can effectively reduce the amylose content of rice and improve the nutritional and eating quality of rice. In particular, the half-life of hydrogen gas in nanobubble hydrogen water is longer, which is highly suitable for the actual situation in agricultural production.

[0070] The above embodiments are merely preferred specific examples of the present application, and are used only to illustrate the technical solutions of the present application and are not intended to limit the present application. Any technical solutions that can be obtained by those skilled in the art based on the spirit of the present application through logical analysis, reasoning, or limited experiments should fall within the scope of the present application.

Claims

1. A method for irrigating rice paddies using nanobubble hydrogen water, The method for reducing the amylose content of paddy rice, wherein the dissolved hydrogen concentration in the nanobubble hydrogen water is 1000 to 1200 ppb.

2. The method according to claim 1, wherein the irrigation with nanobubble hydrogen water reduces the amylose content by 4% to 40%.

3. In each season, from the rice planting period to the ripening period, the total amount of irrigation with the nanobubble hydrogen water is 1200 to 5400 m ３ / hm ２ The method of claim 1, wherein

4. The method according to claim 1, wherein the number of times of irrigation using the nanobubble hydrogen water is 3 to 8 times each season from the rooting stage to the ripening stage of rice.

5. The method according to claim 1, wherein the nanobubble hydrogen water has a diameter of 30 to 600 nm.

6. The method according to claim 1, wherein the nanobubble hydrogen water has a diameter of 30 to 500 nm.

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