Acne treatment composition

Abstract

To provide means for improving skin conditions such as acne.SOLUTION: A composition for improving acne contains a proteoglycan. Preferably, the proteoglycan is a chondroitin sulfate-based proteoglycan. Preferably, the proteoglycan is a salmon nasal cartilage-derived proteoglycan. The composition is applied in the form of, for example, an external preparation for skin.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to novel uses of proteoglycans (such as cosmetics and external skin preparations such as quasi-drugs) based on the porphyrin-reducing effect of proteoglycans in human skin. [Background technology]

[0002] It is believed that acne can be improved by reducing (inhibiting) porphyrins in human skin (Patent Document 1).

[0003] Furthermore, compositions containing proteoglycans and the like are believed to have the effect of improving the skin (particularly improving the skin's moisture retention) (Patent Document 2). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent Publication No. 2004-161641 [Patent Document 2] Patent Publication No. 2019-11316 [Patent Document 3] Patent Publication No. 2013-049634 Summary of the Invention [Problem to be solved by the invention]

[0005] Currently, there is a demand for more effective means of improving skin conditions, such as acne, in humans.

[0006] Therefore, an object of the present invention is to provide a means for improving skin conditions, such as acne, in human skin. [Means for solving the problem]

[0007] The present invention was made to solve such problems, and when a composition containing proteoglycan was applied to human skin, a decrease in porphyrin was confirmed, and the possibility of improving acne was newly discovered.

[0008] The present invention relates to, for example, a composition for improving acne containing a proteoglycan. Preferably, the proteoglycan is a chondroitin sulfate proteoglycan, and more preferably, the proteoglycan is derived from salmon nasal cartilage. The composition may be in the form of, for example, a topical skin preparation. [Effects of the Invention]

[0009] By using this composition, skin improvement such as acne reduction can be achieved in humans and other skin. DETAILED DESCRIPTION OF THE INVENTION

[0010] The present invention will be described below.

[0011] (proteoglycan) Proteoglycans are glycoproteins composed of a core protein covalently bound to sugar chains called glycosaminoglycans (GAGs), such as chondroitin sulfate and dermatan sulfate. Proteoglycans are one of the major components of the extracellular matrix and are widely distributed throughout the body, including in skin and cartilage. GAG chains have a long, unbranched, linear structure. They are negatively charged due to the large number of sulfate and carboxyl groups they contain, and the electrical repulsion between them causes the GAG ​​chains to assume an extended shape. Furthermore, proteoglycans can retain large amounts of water due to the water affinity of sugars. The numerous GAG chains contained in proteoglycans retain water flexibly like a sponge, providing cartilage with its unique functions of elasticity and shock resistance. The core protein of proteoglycans has the property of binding to various molecules in the matrix. Cartilage proteoglycans have binding domains at the N-terminus that bind to hyaluronic acid and link proteins, and can bind to these substances or even associate with each other in the same molecule. The C-terminus contains lectin-like domains and EGF-like domains that bind to various other molecules. This property allows proteoglycans to build structures that are suited to each tissue. Among proteoglycans, chondroitin sulfate proteoglycans are proteoglycans in which chondroitin sulfate is covalently bound to a core protein. For example, salmon nasal cartilage-derived proteoglycan is a proteoglycan extracted from salmon nasal cartilage. Here, salmon is, for example, a fish belonging to the genus Oncorhynchus, and preferably salmon with the scientific name "Oncorhynchus keta" is selected. Furthermore, with regard to the content of proteoglycan contained in the composition of the present invention, for example, from the viewpoint of effect (such as porphyrin reduction), the lower limit is preferably 0.0001 mg / mL or more, more preferably 0.001 mg / mL or more, more preferably 0.01 mg / mL or more, and even more preferably 0.04 mg / mL or more, and from the viewpoint of safety of application to the skin, for example, the upper limit is preferably 300 mg / mL or less, more preferably 100 mg / mL or less, more preferably 10 mg / mL or less, and even more preferably 1 mg / mL or less. The proteoglycan contained in the composition of the present invention is prepared by the method described in, for example, Japanese Patent No. 6317053.

[0012] (Acne improvement) Acne is an inflammatory skin disease (also known as acne vulgaris) caused by various factors. It occurs primarily on the face, head, chest, and back, where there are many sebaceous glands. It is believed that the onset of acne involves a complex interplay of various factors, including excessive sebum secretion, hair follicle keratinization, resident skin bacteria such as Propionibacterium acnes, and bacteria within hair follicles. Therefore, creams and ointments containing sebum secretion-inhibiting ingredients, keratolytic ingredients, anti-inflammatory ingredients, and antibacterial substances are commonly used as topical agents for treating acne (Patent Document 3).

[0013] Porphyrin is a phototoxic substance produced by the metabolic process of acne bacteria. In human skin, porphyrin generates singlet oxygen (reactive oxygen) when exposed to ultraviolet light, which worsens acne. Therefore, reducing porphyrin in human skin can prevent acne from worsening (improving acne).

[0014] (External skin preparation form) The topical skin preparation, which is one form of the composition according to the present invention, may be in an appropriate form for use such as ampoules, capsules, powders, granules, liquids, gels, foams, emulsions, sheets, mists, sprays, etc., and may be in the form of 1) pharmaceuticals, 2) quasi-drugs, 3) topical or whole body topical skin preparations (for example, skin lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, solid soaps, liquid soaps, hand washes, and other face washes and skin cleansers, massage preparations, cleansing preparations, hair removal preparations, depilatories, shaving preparations, aftershave lotions, preshave lotions, shaving creams, foundations, lipsticks, blushers, eye shadows, and other cosmetic products. makeup cosmetics such as eyeliner and mascara, perfumes, nail polish, nail enamel, nail enamel remover, poultices, plasters, tapes, sheets, patches, aerosols, etc.); 4) medicinal and / or cosmetic preparations to be applied to the scalp and hair (for example, shampoos, rinses, hair treatments, pre-hair treatments, permanent solutions, hair dyes, hair styling products, hair tonics, hair growth and tonics, poultices, plasters, tapes, sheets, aerosols, etc.); 5) bath additives to be added to bathwater; 6) other products such as underarm odor prevention agents, deodorants, antiperspirants, hygiene products, sanitary cotton, wet tissues, etc.

[0015] (Components of topical skin preparations) Furthermore, such agents can be prepared by arbitrarily selecting and combining the components and additives exemplified below as necessary within the range that does not impair the effects of the present invention. The amount of these to be added to the formulation is not particularly limited, but it is generally considered that about 0.0001 to 50% is preferable.

[0016] (1) Various oils and fats Avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange roughage oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acids, kukui nut oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, Japan wax, coconut oil, beef tallow, lard, squalene, squalane, pristane, and hydrogenated products of these oils and fats (such as hardened oils).

[0017] (2) Waxes Beeswax, carnauba wax, spermaceti, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax, etc.

[0018] (3) Mineral oil Liquid paraffin, Vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, etc.

[0019] (4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, and lanolin fatty acids; and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

[0020] (5) Alcoholic beverages Natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and phenoxyethanol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

[0021] (6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol, and the like.

[0022] (7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

[0023] (8) Metal soaps Examples of such stearate include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylenate.

[0024] (9) Gums, sugars, or water-soluble polymers Gum arabic, gum benzoin, gum dammar, guaiac butter, Irish moss, gum karaya, gum tragacanth, carob gum, quince seed, agar, casein, lactose, fructose, sucrose or its esters, trehalose or its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide Examples of the polymer include polyalkylene oxides such as olefins and polypropylene oxides, crosslinked polymers thereof, carboxyvinyl polymers, and polyethyleneimine.

[0025] (10) Surfactants Anionic surfactants (alkyl carboxylates, alkyl sulfonates, alkyl sulfates, alkyl phosphates), cationic surfactants (alkylamine salts, alkyl quaternary ammonium salts), amphoteric surfactants: carboxylic acid amphoteric surfactants (amino type, betaine type), sulfate ester amphoteric surfactants, sulfonate amphoteric surfactants, phosphate ester amphoteric surfactants, nonionic surfactants (ether type nonionic surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants, protein hydrolysate derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants), etc.

[0026] (11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), Vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol, Vitamin C group: vitamin C acid or its derivatives, Vitamin D group: ergocalciferol Vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin P group (rutin, eriocitrin, hesperidin), vitamin U, etc.

[0027] (12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine, and the like, as well as amino acid derivatives thereof such as sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acid.

[0028] (13) Various additives derived from plant or animal sources These may be selected from a variety of materials and provided after undergoing conventional processing (e.g., crushing, milling, washing, hydrolysis, fermentation, refining, pressing, extraction, fractionation, filtration, drying, powdering, granulation, dissolution, sterilization, pH adjustment, deodorization, bleaching, etc.) according to the type and form of the product to which they are to be added. [Example]

[0029] [Evaluation of skin condition improvement effects such as porphyrin reduction in human monitor studies] (Test Method) This monitoring test was conducted on a total of 20 healthy male and female subjects aged 20 to 59 (9 males, with an average age of 47.4 years, and 11 females, with an average age of 42.1 years). The lotions with the compositions shown in Table 1 were applied to the left half of the subjects' faces at predetermined intervals, and the lotions shown in Table 2 were applied to the right half of the subjects' faces at predetermined intervals. These application intervals were twice daily (morning and evening) for four weeks. The full amounts of the lotions shown in Table 1 and Table 2 were used over the four-week period. Measurements of the applied skin (dermal collagen density, wrinkle measurement, skin moisture content, and porphyrin measurement) were then performed at week 0 before application of these lotions and at week 4 after application of the lotions.

[0030] The results of the measurements are shown in Tables 3 to 6. In Tables 3 to 6, "Table 1 Application Group" indicates the group to which a lotion having the composition shown in Table 1 was applied, "Table 2 Application Group" indicates the group to which a lotion having the composition shown in Table 2 was applied, "Week 0" indicates that the measurement was taken before the lotion was applied, and "Week 4" indicates that the measurement was taken 4 weeks after the lotion was applied. The measurement results shown in Tables 3 to 6 were calculated (as relative values) by setting the average value of the measurement results (measured at least once for each measurement) of the subjects at "Week 0" in each application group to "100.00."

[0031] [Table 1]

[0032] [Table 2]

[0033] Dermal collagen density was measured using a DermaLab® Combo, and the results are shown in Table 3 below. Measurements taken at the side of the nose confirmed a significant (p<0.01 by t-test) increase in dermal collagen density in the Table 1 application group (the group that received a lotion containing proteoglycan) at week 4 compared to week 0. Furthermore, at week 4, a significant (p<0.05 by t-test) increase in dermal collagen density was confirmed in the Table 1 application group compared to the Table 2 application group (the group that received a lotion not containing proteoglycan). Measurements of the lower eyelid revealed a significant (p<0.05 by t-test) increase in dermal collagen density at 4 weeks compared to 0 weeks in the Table 1 application group (the group that applied a lotion containing proteoglycan).

[0034] [Table 3]

[0035] The wrinkles were measured by counting the number of wrinkles on half of the face (from the lower eyelid to the entire cheek). TM Measurements were performed using Evolution (VISIA-Evo, Canfield Scientific). The results are shown in Table 4. In the Table 1 application group (the group that received a lotion containing proteoglycan), a significant reduction in the number of wrinkles was confirmed at 4 weeks compared to 0 weeks (t-test, p<0.05). Furthermore, at 4 weeks, a significant reduction in the number of wrinkles was confirmed in the Table 1 application group compared to the Table 2 application group (the group that received a lotion not containing proteoglycan) (t-test, p<0.05).

[0036] [Table 4]

[0037] Skin moisture content was measured using MoistureMAP MM100 (Courage+Khazaka electronic GmbH, analysis was performed using GrayIndexT). The measurement results are shown in Table 5 below. At 4 weeks, a significant increase in skin moisture content was confirmed in the Table 1 application group compared to the Table 2 application group (t-test, p<0.05). The measurement results suggest that applying proteoglycan to the skin has a moisturizing effect on the skin.

[0038] [Table 5]

[0039] Porphyrin measurement with VISIA TM The measurement was performed using Evolution (VISIA-Evo, Canfield Scientific). The measurement results are shown in Table 6 below. In the Table 1 application group (the group that applied a lotion containing proteoglycan), a significant (p<0.05 by t-test) decrease in porphyrin in the skin was confirmed at 4 weeks compared to 0 weeks. At 4 weeks, a significant (p<0.05 by t-test) decrease in porphyrin in the Table 1 application group was confirmed compared to the Table 2 application group. The measurement results suggest that applying proteoglycan to the skin has the effect of improving acne on the skin.

[0040] [Table 6]

[0041] The above has described the embodiments of the present invention (including examples), but the specific configuration of the present invention is not limited to this, and even if there are design changes, etc., within the scope that does not deviate from the gist of the present invention, they are included in the present invention.

Claims

1. A topical skin preparation for reducing porphyrins, containing 0.01 mg / mL to 1 mg / mL of chondroitin sulfate proteoglycan derived from salmon nasal cartilage.

2. The agent according to claim 1, further for increasing dermal collagen density.

3. The agent according to claim 1 or 2, further for inhibiting wrinkles.

4. The agent according to any one of claims 1 to 3, further for moisturizing the skin.

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