Antioxidants or skin stress suppressants

Abstract

An objective of the present invention is to provide a novel antioxidant or skin stress inhibitor. Provided are: an antioxidant or skin stress inhibitor containing a wavelength-converting material as an active ingredient; a composition and product containing said antioxidant or skin stress inhibitor; and a method, for inhibiting oxidation of the skin and / or skin stress, using the same. According to the present invention, it is possible to effectively utilize ultraviolet radiation to inhibit oxidation of skin cells and / or skin stress, thereby salubriously affecting the skin.

Description

[Technical Field]

[0001] The present invention relates to antioxidants or skin stress inhibitors containing wavelength converting substances, compositions and products containing such antioxidants or skin stress inhibitors, and methods for inhibiting oxidation and / or skin stress in the skin using them. [Background technology]

[0002] The harmful effects of ultraviolet rays on the skin include skin cancer, photoaging, age spots, wrinkles, and inflammation, and are undesirable from the standpoint of health and beauty.

[0003] Therefore, many measures are taken to protect the skin from UV rays, such as using sunscreen, staying indoors to avoid exposure to sunlight, using UV-blocking hats and clothing, and using UV-blocking film. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent No. 6424656 [Patent Document 2] Patent No. 6361416 [Patent Document 3] International Publication No. 2018 / 004006 [Patent Document 4] Japanese Patent Application Publication No. 2018-131422 [Patent Document 5] Japanese Patent Application Publication No. 5-117127 [Patent Document 6] Patent No. 4048420 [Patent Document 7] Patent No. 4677250 [Patent Document 8] Patent No. 3303942 [Patent Document 9] Japanese Patent Application Laid-Open No. 2017-88719 [Patent Document 10] International Publication No. 2018 / 117117 Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a novel antioxidant or skin stress inhibitor that utilizes modulation of the wavelength of ultraviolet light. [Means for solving the problem]

[0006] The present inventors have conducted extensive research into how ultraviolet light can be effectively used on the skin. As a result, they discovered that when skin cells are irradiated with ultraviolet light via a wavelength conversion substance that converts the wavelength of ultraviolet light, the expression of oxidative stress-related proteins changes, leading to the idea of ​​an antioxidant or skin stress inhibitor containing a wavelength conversion substance.

[0007] The present application provides the following inventions. (1) An antioxidant or skin stress suppressant containing a wavelength converting substance as an active ingredient, The wavelength conversion substance is an antioxidant or a skin stress inhibitor that converts the wavelength of ultraviolet rays contained in incident light and emits outgoing light with a wavelength longer than the wavelength of the ultraviolet rays. (2) The antioxidant or skin stress suppressant according to (1), wherein the ultraviolet light has a peak wavelength in the range of 200 nm to 400 nm. (3) The antioxidant or skin stress suppressant according to (1) or (2), wherein the emitted light has a peak wavelength in the range of 450 nm to 700 nm. (4) The wavelength conversion substance is one or more phycobiliproteins selected from allophycocyanin, C-phycocyanin, R-phycocyanin, phycoerythrocyanin, B-phycoerythrin, b-phycoerythrin, C-phycoerythrin, and R-phycoerythrin; one or more inorganic phosphors selected from zinc oxide phosphor, magnesium titanate phosphor, and calcium phosphate phosphor; vitamin A, beta-carotene, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, niacin, lycopene, gardenia, safflower, turmeric, cochineal, perilla, red cabbage, flavonoids, carotenoids An antioxidant or skin stress inhibitor according to any one of (1) to (3), comprising one or more components selected from the group consisting of quinoids, porphyrins, anthocyanins, and polyphenols; and / or one or more pigments selected from Red No. 401, Red No. 227, Red No. 504, Red No. 218, Orange No. 205 P, Yellow No. 4, Yellow No. 5, Green No. 201, pyranine concentrate, Blue No. 1, 2,4-diaminophenoxyethanol hydrochloride, Alizurin Purple SS, Purple No. 401, Black No. 401, Herringbone Pink, Yellow No. 401, Benzidine Yellow G, Blue No. 404, Red No. 104, and meta-aminophenol. (5) The antioxidant or skin stress inhibitor according to (4), wherein the wavelength converting substance comprises one or more phycobiliproteins selected from allophycocyanin, C-phycocyanin, R-phycocyanin, phycoerythrocyanin, B-phycoerythrin, b-phycoerythrin, C-phycoerythrin, and R-phycoerythrin; one or more inorganic phosphors selected from zinc oxide phosphor, magnesium titanate phosphor, and calcium phosphate phosphor; and / or one or more vitamin B selected from vitamin B1, vitamin B2, vitamin B6, and vitamin B12. (6) A composition containing the antioxidant or skin stress suppressant according to any one of (1) to (5). (7) The composition according to (6), which is a composition for external use on the skin and is intended to suppress oxidation and / or skin stress in the skin by exposing the skin to light containing ultraviolet rays. (8) Applying the composition according to (6) or (7) to the skin of a subject; and exposing the skin after application of the composition to light containing ultraviolet light; A cosmetic method for inhibiting oxidation and / or skin stress in the skin of a subject. (9) A product containing the antioxidant or skin stress suppressant according to any one of (1) to (5). (10) The product described in (9) is intended to suppress oxidation and / or skin stress in the skin by passing light containing ultraviolet rays through the product and then exposing the skin to the light. (11) Passing light, including ultraviolet light, through the product described in (9) or (10); and directing the transmitted light onto the skin of the subject; A cosmetic method for inhibiting oxidation and / or skin stress in the skin of a subject. [Effects of the Invention]

[0008] The present invention is based on the finding that UV rays can be effectively utilized to suppress oxidation and / or skin stress in skin cells, thereby providing beneficial effects on the skin. The present invention also provides new uses for the aforementioned compounds, which have traditionally been used primarily as dyes, pigments, UV scattering agents, UV absorbers, nutritional ingredients, antioxidants, etc. Furthermore, the present invention may also lead to improved quality of life, such as encouraging people who have previously avoided UV rays for beauty or health reasons to go outside more often. [Brief explanation of the drawings]

[0009] [Figure 1] Figure 1 is a schematic diagram of Experiment 1. DETAILED DESCRIPTION OF THE INVENTION

[0010] The antioxidant or skin stress suppressant of the present invention contains a wavelength converting substance as an active ingredient. The wavelength converting substance refers to a substance that converts the wavelength of ultraviolet rays contained in incident light and emits outgoing light with a wavelength longer than that of the ultraviolet rays.

[0011] Ultraviolet rays may include UVA, UVB, UVC, etc. In one embodiment, ultraviolet rays are light with a peak wavelength between 200 nm and 400 nm. Also, ultraviolet rays may be contained in incident light, such as sunlight. Alternatively, the incident light may be ultraviolet rays, or artificially generated ultraviolet rays may be used. Ultraviolet rays can have various effects on the skin. For example, ultraviolet rays are known to cause sunburn, such as suntan, and to generate reactive oxygen species and cause DNA damage in cells.

[0012] The emitted light emitted by the wavelength conversion material has a wavelength longer than ultraviolet light, preferably having a peak wavelength between 500 nm and 700 nm. The emitted light may have one or more peaks at, for example, but not limited to, 450 nm, 460 nm, 470 nm, 480 nm, 490 nm, 500 nm, 510 nm, 520 nm, 530 nm, 540 nm, 550 nm, 560 nm, 570 nm, 580 nm, 590 nm, 600 nm, 610 nm, 620 nm, 630 nm, 640 nm, 650 nm, 660 nm, 670 nm, 680 nm, 690 nm, 700 nm, or within any range of these values, or may be red light, orange light, green light, blue light, etc. In one embodiment, the wavelength converting substance emits light with a dominant wavelength of 450 nm to 700 nm, for example, 500 nm to 700 nm, when excited with excitation light of 200 nm to 400 nm.

[0013] Examples of wavelength-converting substances include the following: phycobiliproteins such as allophycocyanin, C-phycocyanin, R-phycocyanin, phycoerythrocyanin, B-phycoerythrin, b-phycoerythrin, C-phycoerythrin, and R-phycoerythrin; natural or synthetic ingredients such as vitamin A, beta-carotene, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, niacin, lycopene, gardenia, safflower, turmeric, cochineal, perilla, red cabbage, flavonoids, carotenoids, quinoids, porphyrins, anthocyanins, and polyphenols; and Red No. 401, Red No. 227, Red No. 504, Red No. 218, Orange No. 205 P, Yellow No. 4, Yellow No. 5, Green No. 201, pyranine concentrate, and Blue No. 1. , 2,4-diaminophenoxyethanol hydrochloride, Alizurin Purple SS, Purple No. 401, Black No. 401, Herringdon Pink, Yellow No. 401, Benzidine Yellow G, Blue No. 404, Red No. 104, meta-aminophenol and other pigments; phosphors doped with inorganic compounds to give them fluorescence, for example, the blue phosphor containing amorphous silica particles, cerium, phosphorus and / or magnesium described in Japanese Patent No. 6424656 and the red phosphor containing a compound obtained by activating a mixed crystal of an alkaline earth metal sulfide and a gallium compound with europium described in Japanese Patent No. 6361416, zinc oxide phosphors described in International Publication No. 2018 / 004006, zinc oxide phosphors described in Japanese Patent Application Publication No. 2018-131422, inorganic phosphors described in Japanese Patent Application Publication No. 5-117127, and the like. In one embodiment, the inorganic phosphor is ZnO:Zn, Zn 1+z ,ZnO 1-x The phosphor is one or more phosphors selected from phosphors described in WO 2018 / 004006 in which zinc oxide, which can be expressed as follows, is doped with a sulfur-containing compound, for example, a sulfide salt such as zinc sulfide or zinc sulfate and / or a sulfate salt; magnesium titanate phosphors in which magnesium titanate, such as MgTiO3 or Mg2TiO4, is doped with manganese; and calcium phosphate phosphors in which calcium phosphate, such as Ca(H2PO4)2, CaHPO4 or Ca3(PO4)2, is doped with cerium.

[0014] Wavelength conversion substances may be obtained by methods such as extraction from natural products such as animals, plants, and algae, or by artificial methods such as chemical synthesis. For example, phycobiliproteins may be prepared by extracting algae such as blue-green algae like Spirulina platensis or red algae like Porphyridium purpureum using methods described in, for example, Patent Nos. 4048420, 4677250, and 3303942. Zinc oxide phosphors may be produced using methods such as those described in International Publication No. 2018 / 004006, JP 2018-131422, and JP 5-117127. Magnesium titanate phosphors may be produced using methods described in JP 2017-88719. Calcium phosphate phosphors may be produced using methods described in International Publication No. 2018 / 117117.

[0015] These wavelength conversion substances may be composed of or contain the components exemplified above, and may be used alone or in combination, as long as they do not impair the wavelength conversion effect of the present invention. For example, the above-mentioned phycobiliproteins or inorganic phosphors may be mixed with other wavelength conversion substances, such as vitamin B (vitamin B1, vitamin B2, vitamin B6, vitamin B12, etc.), to achieve a synergistic effect. However, these components are merely examples, and any substance that exhibits the wavelength conversion effect of the present invention may be used.

[0016] Furthermore, the content of the wavelength converting substance in the antioxidant or skin stress suppressant, composition, or product of the present invention is not particularly limited as long as it does not impair the wavelength conversion effect of the present invention, and can be determined appropriately depending on the type of wavelength converting substance and the use of the antioxidant or skin stress suppressant or composition. For example, it can be any content within the range of 0.01 to 99.99% by weight, 0.1% to 999% by weight, etc.

[0017] Skin stress refers to oxidative stress experienced by cells in the skin, particularly the epidermis and dermis. Skin cells are typically damaged by reactive oxygen species (ROS), leading to inflammation and impaired skin barrier function. Therefore, a state of high skin stress refers to a state in which skin cells accumulate damage caused by reactive oxygen species. Reactive oxygen species are generated by abnormal reactions in the cellular electron transport system or by ultraviolet light irradiation. When the antioxidant or skin stress inhibitor of the present invention is irradiated with ultraviolet light, emitted light is generated, which alters the expression of oxidative stress-related proteins in skin cells, thereby exerting antioxidant and / or skin stress-inhibiting effects. Examples of oxidative stress-related proteins include AhR, AhRR, CYP1B1, and HMOX1. The antioxidant and / or skin stress-inhibiting effects may be an inhibitory effect on oxidative stress and / or skin stress induced by ultraviolet light, or an inhibitory effect on oxidative stress and / or skin stress induced by causes other than ultraviolet light. The antioxidant or skin stress inhibitor of the present invention can be used on any subject, but may also be applied to subjects exposed to ultraviolet rays outdoors, etc., or subjects experiencing oxidative stress and / or skin stress.

[0018] The aryl hydrocarbon receptor (AhR) is a transcription factor belonging to the bHLH-PAS (Basic Helix-Loop-Helix-Per-Arnt-Sim) family. The AhR is activated by aromatic hydrocarbons such as dioxins, translocates into the nucleus, and functions as a transcription factor. It is also known to be activated by UV light. Activation increases the expression of enzymes involved in xenobiotic metabolism, such as the drug-metabolizing enzyme CYP1 and glutathione S-transferase Y subunit, and activates pathways involved in ROS accumulation. ROS accumulation causes oxidative stress, leading to the production of inflammatory cytokines and oxidative DNA damage.

[0019] The aryl hydrocarbon receptor repressor (AHRR) functions as a repressor of AhR. Increased expression of AHRR suppresses AhR activity, thereby suppressing drug metabolism and ROS accumulation pathways.

[0020] Cytochrome P4501B1 (CYP1B1) is an enzyme belonging to the cytochrome P450 superfamily and is involved in the metabolism of drugs such as polycyclic aromatic hydrocarbons. Its expression is increased in keratinocytes by UV irradiation. It is also activated by AHR and induces the oxidative stress pathway.

[0021] Heme oxygenase 1 (HMOX1) is an enzyme involved in the initial step of heme metabolism. HMOX1 degrades heme to biliverdin. Heme is a prosthetic group essential for life, but heme released from proteins becomes a harmful molecule that generates reactive oxygen species. Therefore, activation of HMOX1 contributes to the reduction of oxidative stress and anti-inflammation. HMOX1 is induced by oxidative stress, such as UV irradiation.

[0022] The antioxidant or skin stress inhibitor and composition of the present invention may be administered in any form, although topical skin preparations such as pharmaceuticals, quasi-drugs, and cosmetics may be preferred to suppress oxidation and / or skin stress in the skin by exposing the skin to light, including ultraviolet light. When the antioxidant or skin stress inhibitor or composition of the present invention is used as a topical skin preparation, the dosage form, application method, and frequency of administration can be determined as desired. For example, it may be applied to the skin in the form of a lotion, spray, oil, cream, emulsion, gel, sunscreen, or tanning agent, regularly or irregularly, for example, once to several times a day, such as in the morning, afternoon, or evening, before anticipated exposure to sunlight, such as going out, engaging in outdoor activities, marine sports, or skiing.

[0023] Furthermore, the antioxidant or skin stress inhibitor and composition of the present invention can be used in combination with any additive selected as needed, such as excipients, preservatives, thickeners, binders, disintegrants, dispersants, stabilizers, gelling agents, antioxidants, surfactants, preservatives, oils, powders, water, alcohols, thickeners, chelating agents, silicones, antioxidants, moisturizers, fragrances, various medicinal ingredients, preservatives, pH adjusters, neutralizers, etc. Furthermore, other antioxidants or skin stress inhibitors may be used in combination to enhance the effects of the present invention.

[0024] The present invention also provides products for suppressing oxidation and / or skin stress in the skin, such as sun visors, hats, clothing, gloves, screen films, window sprays and creams, window materials, and wall materials, which contain the antioxidant or skin stress suppressant of the present invention. As with the above, the use of additives and the form of the product in the product of the present invention are also optional.

[0025] The present invention also provides a method for producing the antioxidant or skin stress inhibitor, composition, or product of the present invention. Also provided is a method for inhibiting oxidation and / or skin stress in a subject's skin, which method comprises applying the antioxidant or skin stress inhibitor or composition of the present invention to the subject's skin and exposing the applied skin to light containing ultraviolet light; or alternatively, passing light containing ultraviolet light through the product of the present invention and exposing the passed light to the subject's skin. The antioxidant or skin stress inhibitor, composition, or product converts the wavelength of ultraviolet light contained in the incident light to emit outgoing light with a wavelength longer than the wavelength of the ultraviolet light, preferably passing ultraviolet light having a peak wavelength of 200 nm to 400 nm as light having a peak wavelength of 450 nm to 700 nm, for example, 500 nm to 700 nm. The method for inhibiting oxidation and / or skin stress in a subject's skin is intended for cosmetic purposes and may not be a treatment used by doctors or medical professionals. The present invention also provides a beauty counseling method for supporting a subject's beauty practices, which includes presenting the beauty method, antioxidant or skin stress inhibitor, composition or product of the present invention to the subject. [Example]

[0026] The present invention will now be described in more detail with reference to examples, although the present invention is not limited to these examples.

[0027] Experiment 1: Changes in gene expression due to the application of various wavelength conversion substances Experiment 1-1: Preparation of wavelength conversion material The wavelength converting material was prepared as follows. (1) C-Phycocyanin C-phycocyanin (Lina Blue) was obtained from an extract of Spirulina platensis, and its absorption spectrum had a peak wavelength at 350 nm, and its emission spectrum had peak wavelengths at 640 nm and 700 nm. (2) Riboflavin (vitamin B2) Riboflavin, also known as vitamin B2, has an absorption spectrum with a peak wavelength at 445 nm and an emission spectrum with a peak wavelength at 530 nm. (3) Zinc oxide phosphor Lumate G manufactured by Sakai Chemical Industry Co., Ltd. was used. Lumate G is a zinc oxide phosphor obtained by doping ZnO with a sulfur-containing compound and then calcining it, as described in International Publication No. 2018 / 004006. Its absorption spectrum had a peak wavelength at 365 nm, and its emission spectrum had a peak wavelength at 510 nm. (4) Magnesium titanate phosphor Lumate R manufactured by Sakai Chemical Industry Co., Ltd. was used. Lumate R is a magnesium titanate phosphor in which MgTiO3 is doped with manganese, and its absorption spectrum has a peak wavelength at 365 nm, and its emission spectrum has a peak wavelength in the 660 to 680 nm band. The wavelength conversion materials (1) and (2) were dissolved in water to prepare solutions with concentrations of 1% and 5%. The wavelength conversion materials (3) and (4) were dispersed in alcohol to prepare 5% and 10% dispersions.

[0028] Experiment 1-2: Preparation of cell samples The cell samples were prepared as follows. 1. Normal Human Epidermal Keratinocytes: Human skin keratinocytes manufactured by PromoCell were used. A cell suspension (1 mL) stored in liquid nitrogen was thawed in a water bath (37°C) until a small ice pellet remained, and then diluted with 9 mL of warm KGM medium. 2. The dilutions were mixed gently and then transferred to T75 flasks and incubated overnight at 37°C. 3. The next day, the medium was replaced with 10 mL of fresh medium. 4. The medium was changed periodically (every 2-3 days) to allow the cells to continue growing. During this time, the cells were observed under a microscope to confirm that they were growing with the correct morphology. 5. When the cells reached approximately 80% confluence, they were passaged. 6. Cells were passaged by washing them once with 10 mL of warm PBS and then aspirating them. 7. 5 mL of warm trypsin was added to the T75 flask, the bottom of the flask was covered with the trypsin solution, and the flask was left at room temperature for 1 minute before being aspirated. 8. For keratinocytes, the flask was placed in a 37°C oven for a maximum of 5 minutes. The cells were observed under a microscope to confirm that they were small and oval. 9. The side of the T75 flask was then gently tapped to release the cells, and the cells were observed under a microscope to confirm that they were moving freely. 10. Keratinocytes were resuspended in 5 mL of warm trypsin neutralizing solution and transferred to a sterile 50 mL Falcon tube. The flask was rinsed with an additional 5 mL of warm FGM and added to the Falcon tube to ensure all cells were transferred. 11. The cells were centrifuged at 10,000 rpm for 5 minutes (4°C) and the supernatant was removed, being careful not to disturb the cell pellet. 12. Keratinocytes are 4 x 10 4The cells were resuspended in KGM at a concentration of 500 μL cells / well and seeded onto collagen-coated glass-bottom 4-well chamber slides. 13. The medium was changed every 2-3 days and the cells were grown until they reached 60-70% confluence (depending on the type of experiment). 14. 24 hours before irradiation, the medium was changed to one without supplements.

[0029] Experiment 1-3: UV irradiation 1. The solar simulator was turned on and the lamp warmed up at least 30 minutes before irradiation. The solar simulator was set to use the UG11 filter, which passes only UVB and blocks other wavelengths. The UV light that passed through the UG11 filter had a peak wavelength between 300 nm and 385 nm. 2. The temperature control plate was turned on and set to 33°C. 3. The cells prepared in Experiment 1-2 were washed once with warm PBS. 4. Add 0.5 mL of warmed Martinez solution (145 mM NaCl, 5.5 mM KCl, 1.2 mM MgCl2.6H2O, 1.2 mM NaH2PO4.2H2O, 7.5 mM HEPES, 1 mM CaCl2, 10 mM D-glucose) to each well. 5. As shown in Figure 1, the cell wells were placed on a plate, and 0.4 ml of the solution containing the wavelength conversion substances (1) to (4) prepared in Experiment 1-1 was poured into each well of a 24-well plate on top of that, and the plate was placed so as to cover the wells containing the cells. This was done so that the UV light could pass through the solution of the wavelength conversion substance and irradiate the cell solution without the solution of the wavelength conversion substance coming into direct contact with the cell solution. 6. The total is 100mJ / cm 2 As controls, we prepared a sample in which the cells were directly irradiated with UV light without placing a plate of wavelength conversion material on the cell well, and a sample in which the cells were cultured in the dark without being irradiated with UV light. 7. After irradiation, the Martinez solution was replaced with warmed KGM (without supplements) and the plates were returned to a 37°C incubator for 24 hours.

[0030] Experiment 2: Microarray Experiment 2-1: RNA extraction In Experiments 1-3, the cell samples incubated for 24 hours after UV irradiation were washed with 500 μl of warm PBS, and the PBS was completely aspirated. RNA was extracted using the Qiagen RNeasy Mini Kit prep (Qiagen, 74106) according to the manufacturer's instructions.

[0031] Experiment 2-2: Microarray The RNA extracted in Experiment 2-1 was analyzed using a human gene expression microarray, SurePrint G3 Human GE Microarray 8x60K Ver. 3.0 (Agilent Technology). Labeling, amplification, purification, and cRNA quantification of the extracted RNA were performed according to the protocol provided by Agilent Technology, and hybridization samples were prepared. The microarray was observed with an Agilent C Microarray Scanner, and genes whose expression was significantly decreased or increased depending on the presence or absence of wavelength conversion substances were identified and are shown below. [Table 1]

[0032] These results indicate that UV irradiation of wavelength conversion materials changes the gene expression of oxidation- and / or skin stress-related proteins, thereby exerting the effect of suppressing oxidation and / or skin stress.

[0033] The embodiments of the present invention have been described above. However, the present invention is not limited to these and can be modified as needed to include cosmetics, pharmaceutical compositions, etc., without departing from the spirit of the invention.

Claims

1. An antioxidant or skin stress suppressant induced by emitted light due to a wavelength converting substance, comprising a wavelength converting substance as an active ingredient, the wavelength conversion material converts the wavelength of ultraviolet light contained in the incident light and emits output light having a wavelength longer than the wavelength of the ultraviolet light; The wavelength converting substance is c-phycocyanin, riboflavin, zinc oxide phosphor, or magnesium titanate phosphor, an antioxidant, or a skin stress suppressant.

2. applying the antioxidant or skin stress suppressant of claim 1 to the skin of a subject; and irradiating the skin with light containing ultraviolet light after applying the antioxidant or skin stress suppressant; A cosmetic method for inhibiting oxidation and / or skin stress in the skin of a subject.

3. Passing light including ultraviolet light through the antioxidant or skin stress suppressant according to claim 1; and directing the transmitted light onto the skin of a subject. A cosmetic method for inhibiting oxidation and / or skin stress in the skin of a subject.

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