Microbial control on high-frequency contact surfaces in healthcare facilities

Abstract

An improved method for reducing and preventing healthcare associated infections is provided, comprising attaching one or more dry hydrogen peroxide (DHP) generators to a medical procedure area, generating DHP, and maintaining a concentration of between 1.0 parts per billion (ppb) and 200 ppb.

Description

[Technical Field]

[0001] Cross-reference of related applications This application claims priority from U.S. Provisional Patent Application No. 63 / 135,355, filed on 8 January 2021.

[0002] This disclosure relates to a method for providing sustained control of microorganisms, pesticides, and odors in dynamic environments such as functioning hospital wards, and to the application of a method for reducing contamination of high-frequency contact surfaces and decreasing healthcare-associated infection rates. [Background technology]

[0003] Several U.S. patents and apparatuses and methods describe the preparation of dry hydrogen peroxide (DHP), also described as purified hydrogen peroxide gas (PHPG), using a photocatalytic reaction system. An apparatus for the preparation of DHP was first described in U.S. Patent Application Publication No. 2009 / 0041617 ("'617"), published on February 12, 2009. DHP is the unhydrated gas of hydrogen peroxide, having the chemical formula H2O2. As an intrinsic gas, DHP behaves as an essentially ideal gas at standard temperatures and pressures. Like nitrogen, oxygen, and water in the air, DHP gas diffuses freely throughout the environment, limited only by its reactivity. Unlike gases such as nitrogen or carbon dioxide, DHP cannot be compressed and stored for later use due to its high reactivity. DHP gas must be continuously generated in situ and maintained at an effective concentration. In laboratory tests, DHP has shown potent disinfectant activity against various bacteria, fungi, and viruses in the air and on glass and metal surfaces.

[0004] The central element of a device capable of generating measurable amounts of ozone-free DHP is a breathable mesh coated with photocatalytic titanium dioxide (TiO2). In the DHP generator, the "sail" is irradiated with a black light (UVA lamp, 315-400 nm) that drives a photocatalytic reaction between water and oxygen to generate DHP gas. See International Application No. PCT / US2015 / 029276.

[0005] DHP gas has very different properties from vaporized hydrogen peroxide (VHP) prepared from a hydrogen peroxide solution. Hydrogen peroxide in its vaporized or evaporated form is hydrated and does not behave as an ideal gas. Importantly, the vaporized or evaporated form exists at concentrations thousands to millions of hours higher than DHP.

[0006] DHP photocatalyst production is achieved using ambient airflow through an air-permeable catalyst coating mesh, hereafter referred to as the "sail." During operation, absorption of photons at specific catalyst-defining wavelengths generates a reactive ionization region called "plasma" on the catalyst surface. The plasma consists of positive ions and free electrons, as well as hydroxyl radicals, hydroxyl ions, superoxide, ozone ions, hydrogen peroxide, and hydrogen ions. These components are prepared in situ on the irradiated catalyst surface from oxygen and water present in the ambient air. By flowing ambient air through the air-permeable substrate, the plasma components are removed and directed away from the catalyst surface. Thus, the airflow removes the reactive species before they can be consumed. Away from the apparatus, almost all of the reactive species are consumed or decomposed, leaving relatively stable hydrogen peroxide to persist and accumulate in the area outside the apparatus. The DHP generator is designed to prepare hydrogen peroxide gas and direct it to the outside of the apparatus and the surrounding environment. The DHP gas diffuses and mixes freely within the space. Because DHP is highly reactive and degrades as it mixes and diffuses, the area where a single source of DHP can be used is limited.

[0007] DHP generators and methods for using DHP are described in a series of patent publications. International application number PCT / US2014 / 038652, published as international publication number WO2014 / 186805, discloses the efficacy and use of DHP for the control of arthropods, including insects and spiders. International application number PCT / US2014 / 051914, published on February 26, 2015, as international publication number WO2015 / 026958, discloses the beneficial effects of DHP on respiratory health, including increased resistance to respiratory infections in the lungs of mammals and increased low thiocyanate ions. International application number PCT / US2015 / 029276, published on November 12, 2015, as international publication number WO2015 / 171633, discloses improvements to a DHP generator, including an improved sail and catalyst. International application number PCT / US2016 / 028457, published on October 27, 2016, under international publication number WO2016 / 172223, discloses the application of DHP to cleanrooms. International application number PCT / US2016 / 029847, published on November 3, 2016, under international publication number WO2016 / 176486, discloses the use of DHP in agricultural production, transport, and storage. International application number PCT / US2018 / 012984, published on July 12, 2018, under international publication number WO2018 / 129537, discloses the application of DHP to poultry production. The contents of each of the aforementioned applications are incorporated herein by reference in their entirety.

[0008] The previous application documents the achievement of various effects in a controlled laboratory environment. Specific capabilities include reducing levels of microbial contamination by viruses, bacteria, and fungi; reducing levels of volatile organic compounds (VOCs); and controlling insects and spiders (by killing them or by driving them out of the protected area).

[0009] The '617 publication first demonstrated the production of DHP using a photocatalytic device, applied as cultures to steel and glass coupons, and demonstrated its effectiveness against the growth and survival of feline calicivirus, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE), Clostridium difficile, and Aspergillus niger exposed to DHP. While effective under controlled conditions on clean, dry surfaces, the study does not address the activity of DHP when applied to "real-world" conditions or dynamic environments.

[0010] To date, there have been few reports on the effectiveness of DHP outside of controlled laboratory environments, and none have addressed the difficulties in maintaining DHP in dynamic and active environments. In contrast to controlled environments, the "real world" imposes various limitations and challenges. Firstly, target microorganisms exist as complex, interacting populations, comprising different strains of microorganisms. Different levels of cleanliness further complicate the surfaces on which microorganisms reside, varying greatly in terms of dirt, growth resources (e.g., sugars and food) and residual chemicals (e.g., cleaning compounds), which can lead to the selection of resistant organisms or hinder the production and maintenance of DHP. Complex ecosystems often result in different levels of bioburden on target surfaces, requiring different disinfection approaches. While some surfaces resemble sterilized, non-porous steel and glass test surfaces, real-world microbial populations reside on porous surfaces, including fabrics. Furthermore, microorganisms on certain surfaces can form "biofilms" that are resistant to washing and disinfection. See Yang et al., "Combating biofilms," FEMS Immunol Med Microbiol. 65(2):146-57 (2012) and Peterson et al., "Viscoelasticity of biofilms and their recalcitrance to mechanical and chemical challenges," FEMS Microbiol Rev. 39(2):234-45 (2015). These materials and differences generally confuse the effectiveness of disinfectants, and such concerns apply to DHP. As will be discussed later for other disinfection technologies, even when very high mortality rates (6-log or greater) are observed under test conditions, real-world effectiveness is not predictable. Disinfection technologies are regulated under the EPA and FDA's Medical Uses Actual for disinfection claims and related claims, respectively, that relate to human health.

[0011] Healthcare-associated infections (HAIs) are estimated to cost US hospitals more than $30 billion annually. HAIs are extremely common among hospitalized patients, as well as those admitted to long-term care facilities such as nursing homes and advanced nursing facilities. Healthcare-associated infections are becoming an increasingly serious problem worldwide. As the number of antibiotic-resistant bacterial strains, including VRA and MRSA, increases, the threat of HAIs has reached alarming importance. The Centers for Disease Control (CDC) estimates that there are 1.7 million healthcare-associated infections annually, causing 99,000 deaths. The cost of HAIs in US hospitals alone is estimated to be over $30 billion annually in direct patient care costs to hospitals and over $15,275 per infection (see www(dot)cdc(dot)gov / HAI / pdfs / hai / Scott_CostPaper.pdf).

[0012] Existing disinfection methods in hospitals have proven inadequate. This has led to the development of new disinfection methods, including ultraviolet disinfection, vaporized hydrogen peroxide (VHP) systems, and chemical treatments. Recent research suggests that reducing bioburden may lower the risk of HAI (High-Intensity Illness). Rutala and Weber, “Best practices for disinfection of noncritical environmental surfaces and equipment in health care facilities: A bundle approach,” Am J Infect Control 47:A96-A105 (2019), Hayden et al., “Reduction in acquisition of Vancomycin-resistant Enterococcus after enforcement of routine environmental cleaning measures,” Clin Infect Dis.42(11):1552-1560(2006), Denton et al., “Role of environmental cleaning in controlling an outbreak of Acinetobacter baumannii on a neurosurgical intensive care unit”, J Hosp Infect.56(2):106-110(2004), Grabsch et al., “Significant reduction in vancomycin-resistant enterococcus colonization and bacteraemia after "Introduction of a bleach-based cleaning-disinfection program", J Hosp Infect.82(4):234-242(2012), Rampling et al., "Evidence that hospital hygiene is important in the control of methicillin-resistant Staphylococcus aureus", J Hosp Infect.49(2):109-116 (2001), Wilson et al., "The impact of enhanced cleaning within the intensive care unit on contamination of the near-patient environment with hospital pathogens: a randomized crossover study in critical care units in two hospitals," Crit Care Med. 39(4):651-658 (2011), and Eckstein et al., "Reduction of Clostridium difficile and Vancomycin-resistant Enterococcus contamination of environmental surfaces after an intervention to improve cleaning methods," BMC Infect Dis. 7:61 (2007). Furthermore, it is recognized that infection risk is multifactorial, and other factors such as hand hygiene compliance, instrument sterilization, and aseptic techniques may also play a role, making it difficult to isolate a single intervention, such as a reduction in surface bioburden, as the sole causative factor of HAI reduction. See Sullivan et al., "Cleaning Hospital Room Surfaces to Prevent Health Care-Associated Infections: A Technical Brief," Annals of Internal Medicine 163:598-607 (2015).

[0013] Hydrogen peroxide is already well known in both liquid and vapor forms and has been widely used in medicine for many years. Microorganisms are susceptible to the effects of hydrogen peroxide because they require water from the environment and have electrostatically configured points on their cells designed to attract water molecules from the environment. Hydrogen peroxide molecules are structurally similar to water molecules and are also attracted to these points. Unfortunately, when mixed with water, hydrogen peroxide molecules must compete with water molecules to access these points, and higher concentrations of hydrogen peroxide are required to demonstrate the effectiveness when mixed with water. In contrast, DHP is not mixed with water and is not hydrated, so it can achieve effectiveness using low concentrations of hydrogen peroxide.

[0014] In order to achieve greater success in our preventive efforts to reduce HAI, which threatens patients' lives, we clearly need innovative strategies to improve HAI rates, in addition to our current return to basics efforts.

[0015] Given the costs and inconveniences involved, microbial reduction in healthcare settings remains a persistent concern, and a variety of techniques exist for reducing microorganisms in occupied spaces. These include vaporized hydrogen peroxide used alone or in combination with chemical disinfectants such as peracetic acid, ultraviolet (UV) light, and sodium hypochlorite, as well as enhanced manual cleaning. Other approaches incorporate biocides, such as copper, into surfaces. Rivero et al., “Impact of copper in the reduction of hospital-acquired infections, mortality and antimicrobial costs in the Adult Intensive Care Unit” Rev Chilena Infectol.31(3):274-9 (2014), Sifri et al., “Reduced healthcare-associated infections in an acute care community hospital using a combination of self-disinfecting copper-impregnated composite hard surfaces and linens”, Am J Infect Control 44(12):1565-71(2016), Humphreys, “Self-disinfecting and Microbiocide-Impregnated Surfaces and Fabrics:What Potential in Interrupting the Spread of Healthcare-Associated Infection?” Clin Infect Dis.58(6):848-853(2014), Montero et al., “Antimicrobial properties of a novel copper-based composite coating with potential for use in healthcare facilities, Antimicrob Resist Infect Please refer to Control.8:3(2019).

[0016] McDonald and Arduino report that there is increasing attention on high-touch surfaces in healthcare settings, with multidrug-resistant bacteria being a particular concern. High-touch surfaces are defined by the frequency of contact by healthcare workers. See Huslage et al., "A Quantitative Approach to Defining High-Touch Surfaces in Hospitals," Infect Control Hosp Epidemiol. 31:850-853 (2010) and Huslage et al., "Microbial assessment of high-, medium-, and low-touch hospital room surfaces," Infect Control Hosp Epidemiol. 34(2):211-2 (2013). Due to the indirect transmission of pathogens, high-touch surfaces serve as a common intermediate step that can be targeted to reduce transmission.

[0017] Evaluating the effectiveness of disinfection techniques can be a challenging task, complicated by factors including hand hygiene, isolation, equipment, antibiotic use, patient populations, public access, and minority groups. Furthermore, the success of comprehensive efforts to reduce infection, particularly in healthcare settings, makes demonstrating improvements even more difficult. See, for example, McDonald and Arduino, "Climbing the evidentiary hierarchy for environmental infection control," Clin Infect Dis. 56:36e39 (2013).

[0018] Vaporized hydrogen peroxide (VHP) as a disinfectant in the healthcare setting has received various evaluations. In addition to its toxicity, VHP can rapidly and essentially completely kill microorganisms (e.g., 6-log kill), but has been reported numerous times as providing incomplete protection against pathogens such as VRE, MRSA, multidrug-resistant Gram-negative bacteria (MDR GNB), and C. difficile. The toxicity of VHP requires the room to be vented prior to decontamination. Further preparation includes sealing doors and gaps and disabling the heating, ventilation, and air conditioning (HVAC) system to prevent unwanted dilution of the VHP in use. Additionally, the results are sensitive to the use parameters such as the concentration of the undiluted hydrogen peroxide solution. Thus, VHP decontamination can only be achieved as a terminal disinfection (i.e., not for daily disinfection). After treatment, decontamination may require 3–5 hours and the space cannot be occupied. Finally, with significant capital equipment costs, supply costs, and labor, the VHP approach is not very attractive. Rutala and Weber have reported many drawbacks of VHP and UV-C systems. See Rutala and Weber, “Are room decontamination units needed to prevent transmission of environmental pathogens?” Infect Control Hosp Epidemiol. 32:743e747 (2011).

[0019] Despite considerable success, VHP treatment is inadequately effective and therefore has limited applicability to routine disinfection. McDonald and Arduino report a prospective cohort intervention study evaluating the effects of VHP decontamination on environmental contamination and MDRO acquisition compared to standard cleaning protocols. Finding reductions in both, the authors stated, "[Surprisingly] despite the remarkable effectiveness of [VHP] decontamination, 13.9% of rooms were still contaminated after [VHP] decontamination (i.e., Table 5 in the report by Passaretti et al.)." McDonald and Arduino, "Climbing the evidentiary hierarchy for environmental infection control," Clin Infect Dis. 56:36e39 (2013).

[0020] Even when VHP treatment is successful, the environment becomes contaminated rapidly. Passaretti et al. have shown that recontamination after VHP treatment occurs quickly. See Passaretti et al., "An evaluation of environmental decontamination with hydrogen peroxide vapor for reducing the risk of patient acquisition of multidrug-resistant organisms," Clin Infect Dis. 56: 27-35 (2013). Hardy et al. report that even with effective decontamination methods such as VHP, recontamination occurs rapidly within 24 hours, and total bacterial counts can reach pre-VHP levels for up to a week. Hardy et al. conclude that "[VHP] is not effective in controlling environmental levels of either MRSA or total bacterial counts when patients are readmitted to wards without partitions." See Hardy et al., "Rapid recontamination with MRSA of the environment of an intensive care unit after decontamination with hydrogen peroxide vapor," J Hosp Infect. 66: 360 (2007).

[0021] A fogging system using hydrogen peroxide and peracetic acid is effective in reducing and eliminating C. difficile spores, MRSA, and vancomycin-resistant enterococci on carriers placed at multiple locations throughout the hospital room, except for partially open drawers. Mana et al., "Evaluation of an automated room decontamination device using aerosolized peracetic acid", Am J Infect Control 45:327-9 (2017). Generally, similar to the VHP method, the drawbacks of the fogging method include the cost of the solution, the preparation for sealing vents, and the doors to prevent fog leakage. Furthermore, peracetic acid and hydrogen peroxide can cause severe eye, skin, and respiratory irritation, so care is required to ensure personnel safety. The corrosiveness of peracetic acid and VHP further limits their usefulness.

[0022] Ultraviolet light, specifically UV-C wavelengths (290nm-100nm), has shown some promise in decontamination rooms and reducing hospital-acquired infections. Similar to the VHP approach, UV-C light disinfection requires the removal of all patients and staff from the room. Therefore, UV-C can only be used for final disinfection. See Rutala and Weber. Furthermore, UV-C treatment may require the rearrangement of furniture and equipment, and its effectiveness depends on the distance of objects from the UV-C source. See Cadnum et al., "Effect of variation in test methods on performance of ultraviolet-C radiation room decontamination," Infect Control Hosp Epidemiol. 37:555-60 (2016). See also Nerandzic et al., "Sorting through the wealth of options: comparative evaluation of two ultraviolet disinfection systems," PLoS One 23(9):e107444 (2014), and Nerandzic et al., "Evaluation of an automated ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogen in hospital rooms," BMC Infect Dis.10:197 (2010).

[0023] Other reports indicate that proper manual application of chemical disinfectants yields reductions comparable to those achieved with VHP. For example, Po and Carling reported that applying 5,500 ppm sodium hypochlorite to commonly touched environmental surfaces in rooms of patients with C. difficile infection resulted in an overall 97% reduction in environmental contamination, compared to an average of 89% across three VHP studies. See Po and Carling, "The need for additional investigation of room decontamination processes," Infect Control Hosp Epidemiol, 31:776 (2010). Cohort studies evaluating the effectiveness of manual and VHP final washes in removing C. difficile reveal similar shortcomings. Yui et al. reported that while VHP reduced contamination by C. difficile, the reduction was not complete, with 4.4% of surfaces remaining positive after VHP use. See Yui et al., "Identification of Clostridium difficile reservoirs in the patient environment and efficacy of aerial hydrogen peroxide decontamination," Infect Control Hosp Epidemiol. 38:1487-92 (2017).

[0024] Chemical disinfectants such as sodium hypochlorite are as effective as UV-C based approaches, but are often toxic and corrosive. Furthermore, proper application by staff is crucial for successful use. See Nerandzic et al., (2010) and Nerandzic et al., (2014).

[0025] Manual cleaning efforts are plagued by compliance and monitoring issues, even after intervention programs have been implemented that ensure less than 85% of the objects are properly cleaned. See Rutala and Weber. [Prior art documents] [Patent Documents]

[0026] [Patent Document 1] U.S. Patent Application Publication No. 2009 / 0041617 ("'617 Publication") [Patent Document 2] International Application Number PCT / US2015 / 029276 [Patent Document 3] International Publication Number WO2014 / 186805 [Patent Document 4] International Publication Number WO2015 / 026958 [Patent Document 5] International Publication Number WO2015 / 171633 [Patent Document 6] International public access number WO2016 / 172223 [Patent Document 7] International Publication Number WO2016 / 176486 [Patent Document 8] International Publication Number WO2018 / 129537 [Non-patent literature]

[0027] [Non-Patent Document 1] Yang et al., “Combating biofilms,” FEMS Immunol Med Microbiol.65(2):146-57(2012) [Non-Patent Document 2] Peterson et al., “Viscoelasticity of biofilms and their recalcitrance to mechanical and chemical challenges,” FEMS Microbiol Rev. 39(2):234-45 (2015) [Non-Patent Document 3] Rutala and Weber, “Best practices for disinfection of noncritical environmental surfaces and equipment in health care facilities: A bundle approach,” Am J Infect Control 47:A96-A105 (2019). [Non-Patent Document 4] Hayden et al., “Reduction in acquisition of Vancomycin-resistant Enterococcus after enforcement of routine environmental cleaning measures,” Clin Infect Dis.42(11):1552-1560 (2006) [Non-Patent Document 5] Denton et al., “Role of environmental cleaning in controlling an outbreak of Acinetobacter baumannii on a neurosurgical intensive care unit,” J Hosp Infect.56(2):106-110 (2004) [Non-Patent Document 6] Grabsch et al., “Significant reduction in vancomycin-resistant enterococcus colonization and bacteraemia after introduction of a bleach-based cleaning-disinfection program,” J Hosp Infect.82(4):234-242 (2012) [Non-Patent Document 7] Rampling et al., “Evidence that hospital hygiene is important in the control of methicillin-resistant Staphylococcus aureus,” J Hosp Infect. 49(2):109-116 (2001) [Non-Patent Document 8] Wilson et al., “The impact of enhanced cleaning within the intensive care unit on contamination of the near-patient environment with hospital pathogens: a randomized crossover study in critical care units in two hospitals,” Crit Care Med.39(4):651-658 (2011) [Non-Patent Document 9] Eckstein et al., “Reduction of Clostridium difficile and Vancomycin-resistant Enterococcus contamination of environmental surfaces after an intervention to improve cleaning methods,” BMC Infect Dis.7:61 (2007) [Non-Patent Document 10] Sullivan et al., “Cleaning Hospital Room Surfaces to Prevent Health Care-Associated Infections: A Technical Brief,” Annals of Internal Medicine 163:598-607 (2015) [Non-Patent Document 11] Rivero et al., "Impact of copper in the reduction of hospital-acquired infections, mortality and antimicrobial costs in the Adult Intensive Care Unit", Rev Chilena Infectol. 31(3):274-9 (2014) Non-Patent Document 12 Sifri et al., "Reduced healthcare-associated infections in an acute care community hospital using a combination of self-disinfecting copper-impregnated composite hard surfaces and linens", Am J Infect Control 44(12):1565-71 (2016) Non-Patent Document 13 Humphreys, "Self-disinfecting and Microbiocide-Impregnated Surfaces and Fabrics: What Potential in Interrupting the Spread of Healthcare-Associated Infection?", Clin Infect Dis. 58(6):848-853 (2014) Non-Patent Document 14 Montero et al., "Antimicrobial properties of a novel copper-based composite coating with potential for use in healthcare facilities", Antimicrob Resist Infect Control. 8:3 (2019) Non-Patent Document 15 Huslage et al., “A Quantitative Approach to Defining High-Touch Surfaces in Hospitals,” Infect Control Hosp Epidemiol. 31:850-853 (2010) [Non-Patent Document 16] Huslage et al., “Microbial assessment of high-,medium-,and low-touch hospital room surfaces,” Infect Control Hosp Epidemiol.34(2):211-2(2013) [Non-Patent Document 17] McDonald and Arduino, “Climbing the evidentiary hierarchy for environmental infection control”, Clin Infect Dis.56:36e39 (2013) [Non-Patent Document 18] Rutala and Weber, “Are room decontamination units needed to prevent transmission of environmental pathogens?” Infect Control Hosp Epidemiol. 32:743e747 (2011) [Non-Patent Document 19] Passaretti et al., “An evaluation of environmental decontamination with hydrogen peroxide vapor for reducing the risk of patient acquisition of multidrug resistant organisms,” Clin Infect Dis.56:27-35 (2013) [Non-Patent Document 20] Hardy et al., “Rapid recontamination with MRSA of the environment of an intensive care unit after decontamination with hydrogen peroxide vapor,” J Hosp Infect.66:360 (2007) [Non-Patent Document 21] Mana et al., “Evaluation of an automated room decontamination device using aerosolized peracetic acid,” Am J Infect Control 45:327-9 (2017) [Non-Patent Document 22] Cadnum et al., “Effect of variation in test methods on performance of ultraviolet-C radiation room decontamination,” Infect Control Hosp Epidemiol.37:555-60 (2016) [Non-Patent Document 23] Nerandzic et al., “Sorting through the wealth of options: comparative evaluation of two ultraviolet disinfection systems,” PLoS One 23(9):e107444 (2014) [Non-Patent Document 24] Nerandzic et al., “Evaluation of an automated ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens in hospital rooms,” BMC Infect Dis.10:197 (2010) [Non-Patent Document 25] Po and Carling, “The need for additional investigation of room decontamination processes,” Infect Control Hosp Epidemiol, 31:776 (2010) [Non-Patent Document 26] Yui et al., “Identification of Clostridium difficile reservoirs in the patient environment and efficacy of aerial hydrogen peroxide decontamination,” Infect Control Hosp Epidemiol.38:1487-92 (2017) [Overview of the project] [Problems that the invention aims to solve]

[0028] HAIs are extremely common among hospitalized patients, as well as those admitted to long-term care facilities such as nursing homes and advanced nursing facilities. Reducing HAI infections is a long-standing and unmet need in most situations, especially in long-term settings. Preventing HAIs, as required by the Veterans Administration and other regulations, is particularly challenging in critical healthcare settings that are meant to have a home-like environment. Implementing typical infection prevention and control measures, such as contact precautions, is difficult when the message of infection prevention and a home-like environment is contradictory for both healthcare providers and patients. Despite improved hand hygiene compliance and strict isolation precautions, there has been no historical improvement in HAI rates (healthcare-associated infection rates). Daily disinfection using hospital-approved disinfectants is not feasible in such situations due to limited personnel and resources. Automated solutions that help maintain low bioburdens and prevent HAIs have the potential to address a significant unmet need in long-term settings. The application of DHP, a continuous method for reducing microbial load, provides a solution for infections affecting one in ten hospitalized patients. [Means for solving the problem]

[0029] In one embodiment, the present disclosure includes a method for establishing a DHP-containing environment for microbial reduction, comprising: attaching a catalyst sail dry hydrogen peroxide (DHP) generator having a new, unused catalyst sail to an environment for microbial reduction; operating the DHP generator to produce and disperse DHP; adjusting the treatment environment with DHP and providing adjustment time for monitoring VOC and DHP concentrations; and replacing the catalyst sail and establishing a DHP concentration of at least 5.0 ppb in a treatment area, maintaining the DHP concentration at a level of at least 5.0 parts per billion (ppb) to a maximum of 40 ppb for at least one week.

[0030] In another form, the Disclosure includes a method for reducing hospital-acquired infections in a medical treatment area requiring a method for reducing hospital-acquired infections, comprising: operating one or more DHP generators continuously within the treatment area requiring the method; establishing an average DHP concentration of at least 5.0 ppb and distributing it over at least 90% of the volume of the treatment area; maintaining the DHP at an average concentration of at least 5.0 parts per billion to a maximum of 40 ppb for at least one week; and reducing pathogenic bacteria within the medical facility.

[0031] In one embodiment, the Disclosure provides, and includes, a method for reducing multidrug-resistant organisms (MDROs) on the surface of a medical treatment area, comprising: continuously operating one or more dry hydrogen peroxide (DHP) generators to generate and disperse DHP within the treatment area; establishing and dispersing a DHP concentration of at least 5.0 ppb within the treatment area; maintaining the DHP at a concentration of at least 5.0 ppb for at least one week; and reducing the number of multidrug-resistant organisms (MDROs) in the medical facility by at least 70% and maintaining the reduced level for at least 30 days.

[0032] In one embodiment, the Disclosure provides a method for reducing the spread of infection in an acute infectious outbreak in a medical treatment area requiring a method for reducing the spread of infection in an acute infectious outbreak, the method comprising: identifying a treatment area having an acute infectious outbreak; providing one or more dry hydrogen peroxide (DHP) generators for generating and dispersing DHP within the treatment area; establishing a DHP concentration of at least 20 ppb; and maintaining the DHP at a concentration of at least 20 ppb until the acute infectious outbreak is controlled.

[0033] This disclosure is made with reference to the attached drawings. [Brief explanation of the drawing]

[0034] [Figure 1] This is a plot of infection rates in a hospital study showing a decrease in the number and frequency of HAI infections during the study period. [Figure 2] This plots the colony-forming units (CFU) by unit and sample day. [Figure 3] This is a plot of microbial count (CFU) by surface type for each sample day.

[0035] Corresponding reference numerals indicate corresponding parts through several figures. The examples described herein illustrate embodiments of the present disclosure, but should not be construed as limiting the scope of the present disclosure. [Modes for carrying out the invention]

[0036] This disclosure provides, and includes, methods for providing DHP in complex environments, particularly healthcare facilities. Such environments present unique challenges that must be overcome to apply promising DHP technologies to reduce infection, morbidity, and mortality.

[0037] Applying DHP technology to a medical setting presents unique challenges. Factors influencing the effectiveness of DHP in such an environment include: • Safety restrictions limiting the use of DHP in spaces occupied by patients and medical staff. • Some medical treatment areas may include hospital wards with an area of ​​tens of thousands of square feet, thus presenting a size challenge. • Hospital wards are often divided into many smaller spaces provided by general air treatment systems, thus providing compartmentalization. • Recirculation: Recirculating air through a filtration system that destroys a considerable amount of DHP. • Line loss: Long sections of ducts can cause DHP loss on their internal surfaces. • Occupation: Each of the dozens of people in the treatment area consumes up to 30 liters of DHP per minute through respiration. • Equipment and furniture: Equipment and furniture increase the surface area of ​​the DHP reaction and disrupt the airflow in the space. • Disintegration: DHP disintegrates upon contact with any surface, including furniture, equipment, curtains, and many other items present throughout the ward. • Reactions: DHP reacts with VOCs from substances in the environment and from medications, cleaning solutions, ointments, and medical gases used throughout the ward; these reactions must be overcome. ○Accumulated VOCs: VOCs that accumulate over time and remain in the environment. ○VOCs emitted: VOCs emitted daily from standard activities.

[0038] To be effective in an active, dynamic occupying hospital accommodating hundreds of patients, visitors, and staff, several planning conditions must be met. Simply installing one or more DHP generators is not sufficient. Rather, whether in a large hospital or a small facility, the space is divided into multiple individual, isolated subsections or treatment areas. As used herein, a treatment area is defined as an area serviced by a single air handler, where all spaces within an individual environment receive supply air from its dedicated single air handler, and all air recycled within that space is returned to that same air handler. Treatment areas within a single air handler zone are often further subdivided by rooms, walls, and other barriers, and these features must be taken into consideration when designing the system and positioning the DHP generators, generally requiring a higher density DHP supply source.

[0039] In some embodiments, the treatment area can be further subdivided to treat smaller areas, but this configuration is not optimal. For example, if DHP is supplied to only 50% of an individual subsection, the available concentration of DHP within that subsection is diluted during recirculation by untreated air from areas of the subsection that are not supplied with DHP, reducing its effectiveness. However, such a configuration may be useful for controlling individual outbreaks and for short-term, high-concentration DHP repair. Similarly, such a configuration is useful during acute infectious outbreaks.

[0040] To achieve the desired treatment environment, the treatment area is first analyzed, and the number of DHP generators required to supply DHP to the entire environment is determined using an average baseline calculation of one DHP generator per 1400 square feet, but allowing for a maximum of one generator per 1200 square feet, or just one generator per 2000 square feet.

[0041] In one embodiment, the DHP generator is placed in an environment integrated into an HVAC system, and is evenly distributed within the system to provide an area where the effects of multiple point sources of DHP overlap. While the HVAC system installation utilizes existing ventilation engineering, similar results can be achieved by using a standalone DHP generator system.

[0042] Both before and after the system is installed, the treatment area is further inspected to identify stagnation zones and hot spots. Stagnation zones and hot spots can occur under a variety of conditions. a. Compartmentation: Too few or too many compartmentalized spaces can hinder the diffusion of DHP into adjacent spaces, potentially resulting in both hot spots and stagnation zones (DHP discontinuities). In most cases, hot spots are of little concern but can reflect the presence of stagnation zones. Stagnation zones can be addressed by installing supplemental standalone DHP generators or additional HVAC units. In some embodiments, hot spots and stagnation zones can be linked and regulated by increasing airflow between spaces, providing additional ventilation, or removing furniture or equipment. b. Reaction burden: Smaller or larger VOC loads from sources within the treatment area, such as off-gas materials, large amounts of ointments, plasters, or volatile solvents, react with DHP and reduce DHP levels. For efficiency, such burdens can be removed. In some embodiments, an additional DHP generator may be required. c. Burden of equipment and furniture: Less or more cluttered space, including a larger than usual amount of furniture, equipment, and stored items. d. Occupancy burden: A low- or high-population-density area that accommodates fewer or more than the average number of patients, medical staff, and visitors who consume DHP through respiration while in the space. e. Volume: The need for a DHP device is generally calculated by the area, and therefore, adjusting the ceiling to be lower or higher than an 8-foot baseline will decrease or increase the volume of the space to be protected.

[0043] The number of devices required to establish an average DHP concentration of at least 5.0 ppb within the treatment area is determined by several factors. Generally, using the devices described above, a single device is typically sufficient for an area of ​​1,400 square feet (approximately 130 m²) with a 9-foot ceiling. 3 This is sufficient to provide coverage. However, this ratio of the device to volume only provides guidance for achieving the desired DHP concentration. Various factors affect steady-state DHP levels in the real world. Firstly, many facilities have heating, ventilation, and air conditioning systems that draw in fresh air and recirculate it through the system. Both recirculation and fresh air reduce steady-state DHP levels.

[0044] One possible solution for establishing a desired concentration of DHP within a treatment area is to provide an additional maximum volume in the form of an additional unit. While this technique can increase DHP levels, it does not address the problem of dispersion. Generally, it is preferable to integrate DHP generators into heating, ventilation, and air conditioning (HVAC) systems, as DHP generators are already designed to supply conditioned air throughout the entire zone. However, even the best-designed HVAC systems inevitably result in uneven dispersion of conditioned air. Dead zones or DHP-deficient zones form in corners and areas not directly supplied by source and return vents. Airflow tends to flow from the source to the return along the path of least resistance. Furthermore, the placement of barriers to airflow creates dead zones (DHP-deficient zones). Examples of barriers include furniture and equipment present in most treatment areas. Temporary barriers, such as privacy curtains that obstruct the free flow of air, can also be problematic. Importantly, in addition to reducing DHP access and dispersion, barriers act as important reservoirs for microorganisms, including pathogenic organisms.

[0045] Current HVAC systems are not designed to account for DHP distribution and therefore inevitably have zones where DHP is deficient and cannot be improved by increasing flow rates. Firstly, there are limits to the flow rates that can be achieved and still maintain a controlled space within its design parameters (temperature, humidity, etc.). Secondly, since most HVAC systems incorporate one or more filters, the passage of DHP-treated air through the filters results in the reaction and destruction of DHP. Importantly, when installing a DHP device in an HVAC system, the device should be installed downstream of any filtration system to ensure that DHP is supplied to the occupied space. In some embodiments, additional DHP generators can be installed inside the air treatment system itself to reduce the microbial load in ducts and vents. Such arrangements may be particularly beneficial in older hospitals where the air system may be contaminated with Legionnaires' disease, etc. In some embodiments, DHP generators can be installed upstream of HVAC filters to reduce the microorganisms trapped by the filters, but the amount of DHP downstream of such treated filters is significantly reduced, and its contribution to the equilibrium DHP concentration in the occupied space is negligible. Finally, to reduce microbial contamination within the ducts, additional DHP units can be installed in the air return plenum (usually upstream of the filter). Older hospitals and buildings can greatly benefit from these additional installations, although such installations do not contribute to the overall DHP levels within the occupied space. Only DHP units installed downstream of the filter and positioned to minimize line losses, while DHP units are installed in both the HVAC source and return ducts, contribute to the accumulation of DHP within the occupied space. Similarly, the ability to flow recirculated air into the unit to increase DHP concentration is also limited, as the active catalyst sail of the DHP generator degrades the DHP. In some embodiments, fresh, humidified air can be supplied to the DHP unit, but this usually requires redesigning the space and, depending on the season, may need to heat / cool / humidify the new air source before it flows through the DHP unit.

[0046] Other factors affecting DHP levels include the treatment area itself, such as several entrances, halls, doors, windows, types of building materials, the number of occupants, occupant turnover, and maintenance activities (cleaning and cleaning supplies). Surfaces that accumulate static charge particularly deplete DHP. The objective of the treatment space is also related to achieving an average DHP level of at least 5.0 ppb.

[0047] Reaching a steady-state concentration of DHP within a treatment area is a two-step process. Firstly, there is an initial adjustment phase (also called the "development phase") followed by a maintenance or treatment phase. During the initial adjustment of the treatment space, much of the DHP generated is quickly depleted as it reacts with the environment. A significant sink for DHP in the environment is the accumulation level of volatile organic compounds (VOCs). These react with DHP and gradually decompose as the DHP reacts and is depleted. In some embodiments, this adjustment phase is accompanied by a measurable increase in humidity. Similarly, the detectable level of VOCs decreases over time and is generally maintained below the threshold detection level during the maintenance phase. Highly sensitive and affordable tools for measuring VOCs are available and can measure levels down to 0.1 ppm. The DHP equilibrium level can be expected to be low during this adjustment or adaptation period of DHP consumption, ranging from 5 parts per billion to 20 parts per billion. As the accumulated VOC levels degrade, the DHP generator needs to produce enough DHP to degrade the ongoing VOC source and provide enough additional DHP to maintain an average DHP level between 5.0 and 20 parts per billion (ppb). Since VOC sources are diverse and highly varied, the required amount depends on each environment.

[0048] During the adjustment or deployment phase, the treatment area and DHP devices undergo several expected behaviors. Firstly, in a multi-source system, a series of DHP generators function as a composite system that works collectively to serve the entire treatment area. As the gas diffuses from high to low concentrations as required by Fick's first law of diffusion, source loss creates DHP dead space, further reducing DHP concentrations in other areas and thus jeopardizing the success of the system. Clearly, DHP loss allows for microbial regrowth, so monitoring and replacement of faulty devices is essential.

[0049] At the start of deployment, the DHP generator first encounters the accumulated VOC load around the treatment area. VOCs have been observed to consume the majority of the DHP produced by the generator for 1 to 7 days, depending on the degree of the load. During this time, a small increase in relative humidity is often observed as the DHP converts VOCs into carbon dioxide and humidity. On average, this will be 3% to 5%, but under extreme accumulated VOC loads it can reach 10% to 20%. The equilibrium level of DHP can be expected to be low during this DHP consumption period, ranging from 1.0 parts per billion to 20 parts per billion.

[0050] The relative humidity eventually returns to normal levels, and this observation indicates that the accumulated VOCs have been oxidized. In some embodiments, VOCs can be directly monitored to detect the end of this initial VOC reduction phase. Also noteworthy during the adjustment phase is the decrease in ambient odor levels throughout the treatment area as various organic odor substances are decomposed. During this initial period, as the accumulated VOCs are oxidized, the catalyst sail ages more rapidly than usual and should be replaced after one week of operation to ensure that the desired steady-state level can be achieved and maintained.

[0051] This disclosure provides a method for preparing a DHP-containing environment to address regions with high reaction burden. In most environments, a one-week adjustment period is used to reduce the accumulated VOCs and other types of reaction burdens and to accumulate increased levels of DHP. In some embodiments, the adjustment period may be extended up to one month. In some embodiments, an additional week is required for adjustment.

[0052] As accumulated VOCs are removed, the DHP equilibrium level rises to an average concentration of at least 5.0 parts per billion, up to a maximum of 50 ppb. These levels can be maintained indefinitely by regular maintenance of the DHP generator. Lower DHP equilibrium levels within this range can be expected in environments with the highest daily VOC emissions, and lower DHP equilibrium levels can be expected in environments with the lowest daily VOC emissions. In one embodiment, the average DHP concentration is at least 10 ppb. In other embodiments, the average DHP concentration is maintained at at least 20 ppb. As provided by the method, the DHP concentration should be maintained between 5.0 and 50 ppb.

[0053] Once a steady-state level of DHP is achieved, as evidenced by direct DHP measurement, indirect VOC measurement, or chronological relative humidity measurement, the method includes and provides a maintenance phase. During continuous operation in the maintenance phase, the sails should be replaced every 2-3 months. Although sails have been observed to provide sufficient DHP production for longer periods depending on the environment, the risk of microbial regrowth dictates a careful replacement schedule.

[0054] This disclosure provides, and includes, a method for establishing a DHP-containing environment for microbial reduction, comprising: attaching at least one catalyst sail dry hydrogen peroxide (DHP) generator to an environment for microbial reduction; operating the at least one DHP generator to produce and disperse DHP; adjusting the environment with DHP and providing adjustment time for monitoring VOC and DHP concentrations; replacing the catalyst sail to establish a DHP concentration of at least 5.0 ppb in the environment; and maintaining the DHP at a concentration between 1 and 50 parts per billion (ppb) for at least one week. In one embodiment, the method further comprises the step of using a new, unused catalyst sail. In further embodiments, the method further comprises the step of replacing the catalyst sail multiple times depending on the reaction load and occupation load. In certain embodiments, the DHP concentration is maintained between 5.0 and 30 ppb.

[0055] This disclosure describes how to use an electric current device to produce 325 cubic meters (m³). 3 The treatment area shall include, and shall have at least one DHP source per unit. Generally, for practical reasons, DHP generators shall be limited to those that can fit into standard HVAC ducts. Standalone systems shall use sails of similar size so as not to be inconspicuous and not to interfere with hospital or room activities. Thus, DHP generators shall generally have sails of 0.5 to 1 square foot and a discharge rate of approximately 0.004 to 0.051 milliwatts / cm². 2Irradiation occurs between these points. As provided herein, the maximum distance of the DHP source should be no more than 20 meters from the treatment target (e.g., surface and air). In some embodiments, for example, in treatment areas with a high reaction load, the DHP source should be no more than 15 meters from the treatment target (e.g., surface and air). In some embodiments, the maximum distance between DHP sources is 10 meters. The number and configuration of sources should be tested to ensure the generation and maintenance of at least an average of 5.0 ppb of DHP gas in the treatment environment. Preferably, the sources should maintain an average DHP gas concentration in the treatment area between 5.0 and 50 ppb. For safety reasons, DHP levels should be monitored and maintained at a level of 200 ppb or less, taking into account the levels approved by OSHA. Starting at levels of approximately 238 ppb, detection of DHP using approved methods begins to approach the maximum safe level of 1.0 ppm established by OSHA with the use of VHP.

[0056] In embodiments of this specification, the treatment area has at least three DHP sources, each located at an average distance of no more than 20 meters from one another. In one embodiment, the treatment environment is approximately 325 meters 3 (E.g., there is a DHP source for every 130 square meter area (e.g., approximately 1400 square feet) with a ceiling height of 2.5 meters (approximately 8 feet) relative to the total treatable air volume). In one embodiment, each treatment area has at least three DHP sources, each spaced at least 15 meters apart from one another on average. In another embodiment, there are at least four DHP sources within a treatment area, each spaced at least 20 meters apart from one another. In a particular embodiment, the maximum average distance between DHP sources is 10 meters. Depending on the size of the space, additional DHP sources may be added and spaced at least 10, 15, or 20 meters apart from one another on average.

[0057] The approximate number of necessary devices can be determined by the overall size of the treatment area where the area is divided into unit areas. The method provides at least one DHP generator for each unit area between about 100 and about 185 square meters (m 2 ²). In some embodiments, for example, spaces with low occupancy, low generated VOCs, and low line losses can be served with fewer devices. In one embodiment, a device is provided per 110 m 2 . In one embodiment, a device is provided per unit area of about 150 m 2 . In other embodiments, a DHP generating device is provided per unit area of about 175 m 2 . Alternatively, the treatment area can be divided into unit volumes. In embodiments according to the present disclosure, a DHP generating device is provided per unit volume between 275 and 460 cubic meters (m 3 ³). In an embodiment, the unit volume per device is about 300 m 3 ³. In another embodiment, the unit volume is about 350 m 3 ³. In other embodiments, the unit volume is about 400 per device. In particular, the above size constraints apply to each volume where the device is installed within an average of 20 meters from other devices.

[0058] In some embodiments, the method provides isolating the treatment area from the surrounding space. Although inefficient, such an arrangement is made when it is impossible to condition the entire hospital ward or HVAC zone. When isolated, the number of devices is increased such that the average unit area can be as low as 50 or 75 m 2 ². In an embodiment, the unit volume of the device is as low as about 150 or 200 m 3 ³. In practice, 28 - 29 DHP supply sources are installed for a 40,000 square foot treatment area within a medical facility.

[0059] In embodiments of the present disclosure, the treatment area includes an HVAC zone.

[0060] The method further provides a flow of DHP containing air from the DHP supply source. The most efficient configuration is to install the DHP device on or near a diffuser above the air intake vent.

[0061] This specification provides, and includes, methods for reducing hospital-acquired infections (HAIs) in healthcare settings. Hospital-acquired infections, often referred to as HAIs, are infections acquired during hospitalization or while residing in a healthcare facility. Methods for reducing HAIs can be implemented as part of a comprehensive, proactive preventative effort, or provided to settings where infection reduction is needed after the detection of high infection rates or an increase in infection rates.

[0062] Methods for reducing hospital-acquired infections in medical treatment areas include providing one or more dry hydrogen peroxide (DHP) generators for each area between 110 and 185 square meters and for each area with a total volume between 275 and 460 cubic meters; maintaining the medical treatment area at an average concentration of at least 5.0 parts per billion to a maximum of 50 ppb, distributing it over 90% of the treatment area's volume for at least three months; and ensuring that hospital-acquired infections in at-risk patients are reduced by at least 25% as measured over a three-month window. In some embodiments, the DHP gas concentration is maintained between 30 and 60 ppb. In other embodiments, the DHP concentration is less than 60 ppb. In some embodiments of this disclosure, the medical treatment area is a climate-controlled treatment area maintained at a humidity between 30 and 60% and a temperature between 68°C and 73°C. See EC.02.06.01, EP 13.

[0063] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the microbial load on a sampling surface by at least 70% compared to an untreated treatment area of ​​similar size and use. In another embodiment, the microbial load is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period.

[0064] This specification provides, and includes, a method for reducing hospital-acquired infections in a healthcare treatment area, comprising reducing the number of microorganism-positive sampling surfaces by at least 70% compared to an untreated treatment area. In one embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In one embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 70% on the sampling surface and maintaining the reduced level for at least 30 days. In one embodiment, the method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface and maintaining the reduced level for at least 30 days. In one embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 90%. In another embodiment, the bacteria are reduced by 95%, 99.0%, or 99.9%. Once reduced, the bacterial levels are maintained for at least 2 months, 3 months, 6 months, or 1 year. In one embodiment, the method involves applying a high-frequency contact surface to a surface measuring 1 square centimeter (cm²). 2 Reduces microbial levels to less than 5 colony-forming units (cfus) per sample.

[0065] This specification provides, and includes, a method for reducing hospital-acquired infections in an active medical treatment area, comprising reducing the number of microorganism-positive sampling surfaces by at least 70% and reducing the incidence of HAI infection by at least 10% compared to an untreated treatment area. In an embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, and the incidence of HAI infection is reduced by at least 10% when measured over the same period. In an embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 70% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 10%. In an embodiment, the method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 10%. In one embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 90%, and the incidence of HAI infection is reduced by at least 10%. In other embodiments, bacteria are reduced by 95%, 99.0%, and 99.9%, and the incidence of HAI infection is reduced by at least 10%. Once reduced, bacterial levels are maintained for at least 2 months, 3 months, 6 months, or 1 year, and the incidence of HAI infection is reduced by at least 10%. In one embodiment, the method is applied to a high-frequency contact surface over a square centimeter (cm²). 2The method reduces microbial levels to less than 5 colony-forming units (cfus) per area. This method provides and includes a reduction of more than 10% in the incidence of HAI infection in the treated active healthcare environment compared to an untreated area in a similar location over a period of 3 months. In some embodiments, the comparison period is at least 6 months. In other embodiments, the comparison period is at least 1 year. A reduction of at least 20% in the incidence of HAI compared to an untreated active healthcare treatment area is also provided. Also provided and included is a reduction of 30% or more in the incidence of HAI compared to an untreated active healthcare treatment area over a comparison period. In some embodiments, the number of HAIs decreases by between 10 and 20%. In other embodiments, the number of HAIs decreases by between 10 and 50% compared to an untreated active healthcare treatment area over a comparison period. Reductions of 20-40%, 30-40%, 30-50%, 30-60%, or more have also been included. The method further provides a reduction of over 50% compared to the untreated area of ​​active medical treatment during the comparison period.

[0066] This specification provides, and includes, a method for reducing hospital-acquired infections in an active medical treatment area, comprising reducing the number of microorganism-positive sampling surfaces by at least 70% and the incidence of HAI infection by at least 20% compared to an untreated treatment area. In an embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, and the incidence of HAI infection is reduced by at least 20% when measured over the same period. In an embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 70% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 20%. In an embodiment, the method provides reducing the number of microorganism-positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 20%. In one embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 90%, and the incidence of HAI infection is reduced by at least 20%. In other embodiments, bacteria are reduced by 95%, 99.0%, or 99.9%, and the incidence of HAI infection is reduced by at least 20%. Once reduced, bacterial levels are maintained for at least 2 months, 3 months, 6 months, or 1 year, and the incidence of HAI infection is reduced by at least 20%. This method provides and includes a reduction of more than 20% in the incidence of HAI infection in a treated active healthcare environment compared to an untreated area similarly located over a 3-month period. In one embodiment, the comparison period is at least 6 months. In other embodiments, the comparison period is at least 1 year. In one embodiment, the number of HAIs is reduced by between 20% and 30%. In other embodiments, the number of HAIs is reduced by between 20% and 50% compared to an untreated active healthcare treatment area over a comparison period.

[0067] This specification provides, and includes, a method for reducing hospital-acquired infections in an active medical treatment area, comprising reducing the number of microbiota-positive sampling surfaces by at least 70% and the incidence of HAI infection by at least 30% compared to an untreated treatment area. In an embodiment, the number of microbiota-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, and the incidence of HAI infection is reduced by at least 30% when measured over the same period. In an embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of microbiota-positive sampling surfaces in a healthcare facility by at least 70% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 30%. In an embodiment, the method provides reducing the number of microbiota-positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface, maintaining the reduced level for at least 30 days, and reducing the incidence of HAI infection by at least 30%. In one embodiment, the number of microorganism-positive sampling surfaces is reduced by at least 90%, and the incidence of HAI infection is reduced by at least 30%. In other embodiments, bacteria are reduced by 95%, 99.0%, or 99.9%, and the incidence of HAI infection is reduced by at least 30%. Once reduced, bacterial levels are maintained for at least 2 months, 3 months, 6 months, or 1 year, and the incidence of HAI infection is reduced by at least 30%. This method provides and includes a reduction of more than 30% in the incidence of HAI infection in a treated active medical environment compared to an untreated area similarly located over a 3-month period. In one embodiment, the comparison period is at least 6 months. In other embodiments, the comparison period is at least 1 year. In one embodiment, the number of HAIs is reduced by between 30% and 40%. In other embodiments, the number of HAIs is reduced by between 30% and 50% compared to an untreated active medical treatment area over a comparison period.

[0068] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the number of MRSA-positive sampling surfaces by at least 70% compared to an untreated treatment area. In another embodiment, the number of MRSA-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In another embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of MRSA-positive sampling surfaces by at least 70% on the sampling surface in a healthcare facility and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing the number of MRSA-positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface and maintaining the reduced level for at least 30 days. In yet another embodiment, the number of MRSA-positive sampling surfaces is reduced by at least 90%. In yet another embodiment, the bacteria are reduced by 95%, 99.0%, or 99.9%. Once reduced, the bacterial levels are maintained for at least 2 months, 3 months, 6 months, or 1 year. In one embodiment, the method involves applying a high-frequency contact surface to a surface measuring 1 square centimeter (cm²). 2 It reduces MRSA levels to less than 5 colony-forming units (cfus) per sample.

[0069] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the number of aerobic bacterial colonies (ABC) positive sampling surfaces by at least 70% compared to an untreated treatment area. In another embodiment, the number of ABC positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In another embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing ABC positive sampling surfaces by at least 70% on the sampling surface in a healthcare facility and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing ABC positive sampling surfaces in a healthcare facility by at least 80% on the sampling surface and maintaining the reduced level for at least 30 days. Once reduced, the bacterial level is maintained for at least 2 months, 3 months, 6 months, or 1 year. In one embodiment, ABC positive sampling surfaces are reduced by at least 90%. In yet another embodiment, bacteria are reduced by 95%, 99.0%, or 99.9%. In one embodiment, the method involves applying a high-frequency contact surface to a surface measuring 1 square centimeter (cm²). 2 Reduce ABC levels to less than 5 colony-forming units (cfu) per unit.

[0070] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the number of C. difficile-positive sampling surfaces by at least 70% compared to an untreated treatment area. In one embodiment, the number of C. difficile-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In one embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the number of C. difficile-positive sampling surfaces in a medical facility by at least 70% on the sampling surface and maintaining the reduced level for at least 30 days. In one embodiment, the method provides reducing the number of C. difficile-positive sampling surfaces in a medical facility by at least 80% on the sampling surface and maintaining the reduced level for at least 30 days. Once reduced, the C. difficile level is maintained for at least 2 months, 3 months, 6 months, or 1 year. In one embodiment, the number of C. difficile-positive sampling surfaces is reduced by at least 90%. In other embodiments, bacteria are reduced by 95%, 99.0%, and 99.9%. In embodiments, the method is applied to a high-frequency contact surface over a square centimeter (cm²). 2 Reduces C. difficile levels to less than 5 colony-forming units (cfu) per unit.

[0071] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the number of colony-forming units (CFU / plate) per contact plate on a contact plate by at least 70% compared to an untreated treatment area. In one embodiment, the number of CFUs per contact plate is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In one embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the CFUs per contact plate by at least 70% on a sampling surface within a healthcare facility and maintaining the reduced level for at least 30 days. In one embodiment, the method provides reducing the CFUs per contact plate by at least 80% on a sampling surface within a healthcare facility and maintaining the reduced level for at least 30 days. Once reduced, the CFUs per contact plate are maintained for at least 2 months, 3 months, 6 months, or 1 year. In one embodiment, the CFUs per contact plate are reduced by at least 90%. In other embodiments, the CFU per contact plate is reduced by 95%, 99.0%, and 99.9%. In embodiments, the method reduces the CFU level per contact plate on a high-frequency contact surface to 1 square centimeter (cm²). 2 Reduce the number of colony-forming units (CFUs) per colony to less than 5.

[0072] In some embodiments, the number of relative light units (RLUs) per sample is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. Methods and apparatus for measuring RLUs are well known in the art. See Amodio and Dino, "Use of ATP bioluminescence for assessing the cleanliness of hospital surfaces: A review of the published literature (1990-2012)," J Infect Public Health 7(2):92-8 (2014); Omidbakhsh et al., "How Reliable Are ATP Bioluminescence Meters in Assessing Decontamination of Environmental Surfaces in Healthcare Settings?", PLos One 9(6):e99951 (2014); and Turner et al., "Efficacy and Limitations of an ATP-Based Monitoring System," J. of American. Assoc. for Laboratory Animal Science 49(2):190-195 (2010). In some embodiments, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the RLU per sample by at least 70% on a sampling surface or in the air within a healthcare facility and maintaining the reduced level for at least 30 days. In some embodiments, the method provides reducing the RLU per sample by at least 80% on a sampling surface or in the air within a healthcare facility and maintaining the reduced level for at least 30 days. In some embodiments, the RLU per sample air is reduced by at least 90%. In other embodiments, the RLU per sample is reduced by 95%, 99.0%, or 99.9%. The RLU sample may be a surface sample or an air sample.

[0073] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes reducing the microbial population within the medical facility by at least 90% on average when measured at swipe sites 20 times per week for one month, and maintaining the reduced level for at least five weeks. In another embodiment, the reduction in microorganisms is measured and compared over a period of three months, six months, or annually. The method provides reducing the microbial population per sampling surface within the medical facility by at least 95% on the sampling surface, and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing the microbial population per sampling surface within the medical facility by at least 99% on the sampling surface, and maintaining the reduced level for at least 30 days. In yet another embodiment, pathogenic bacteria per sampling surface are reduced by at least 90%. In yet another embodiment, the microbial population per sampling surface is reduced by 95%, 99.0%, or 99.9%. In this embodiment, the microbial community is a pathogenic bacterial community selected from the group consisting of vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus, multidrug-resistant Gram-negative bacteria (MDR GNB), aerobic bacterial colonies (ABC), and Clostridium difficile.

[0074] In some embodiments, the sampling surface is a high-touch surface. See Huslage et al., "A Quantitative Approach to Defining High-Touch Surfaces in Hospitals," Infect Control Hosp Epidemiol. 31:850-853 (2010) and Huslage et al., "Microbial assessment of high-, medium-, and low-touch hospital room surfaces," Infect Control Hosp Epidemiol. 34(2):211-2 (2013). In other embodiments, the sampling surface includes high-touch and medium-touch surfaces. In some embodiments, at least 90% of the sampling surface is a high-touch surface selected from the group consisting of bed rails, head and foot rails, tray tables, supply carts, door knobs, push plates, handles, elevator buttons, switches, keyboards, mice, touchscreens, blood pressure cuffs, privacy curtains, blinds, windowsills, furniture, and bathroom sinks. In one embodiment, 95% of the sampling surface is selected from the group consisting of bed rails, head and footboard rails, tray tables, door knobs, push plates, handles, privacy curtains, furniture, and bathroom sinks. In another embodiment, the sampling surface is selected from the group consisting of bed rails, head and footboard rails, tray tables, door knobs, push plates, handles, privacy curtains, furniture, and bathroom sinks. In yet another embodiment, the treatment area is an ICU, and the high-frequency contact surfaces are selected from the group consisting of bed rails, bed surfaces, and supply carts. In yet another embodiment, the treatment area is a medical / surgical bed, and the high-frequency contact surfaces are selected from the group consisting of bed rails, overbed tables, intravenous pumps, and bed surfaces.

[0075] The Disclosure further provides a method for reducing microorganisms on a privacy curtain, comprising continuously supplying DHP at a DHP concentration of at least 1.0 parts per billion up to a maximum of 50 ppb, thereby reducing the level of microorganisms on the curtain by at least 99%. In one embodiment, the level of microorganisms on the curtain is reduced by at least 99.9%. In one embodiment, the DHP gas concentration is maintained between 1.0 and 40 ppb to reduce microorganisms on the privacy curtain. In one embodiment, the DHP gas concentration is maintained between 5.0 and 20 ppb to reduce microorganisms on the privacy curtain. In another embodiment, the DHP gas concentration is maintained between 5.0 and 40 ppb to reduce microorganisms on the privacy curtain. In yet another embodiment, the DHP gas concentration is maintained between 5.0 and 30 ppb.

[0076] In aspects of this disclosure, the treatment area for the method of reducing hospital-acquired infections is selected from the group consisting of wards, HVAC zones, and air exchange restricted rooms. In other aspects, the treatment area for the method of reducing hospital-acquired infections is selected from the group consisting of intensive care units (ICUs), oncology wards, nephrodialysis units, neonatal ICUs, and pediatric ICUs.

[0077] In one embodiment, a method for reducing hospital-acquired infections in a medical treatment area includes: continuously operating one or more dry hydrogen peroxide (DHP) generators to generate and disperse DHP within the treatment area; establishing a DHP concentration of at least 1.0 parts per billion to a maximum of 50 ppb dispersed over at least 90% of the volume of the treatment area; maintaining DHP at a concentration of at least 5.0 parts per billion to a maximum of 50 ppb for at least one week; reducing pathogenic bacteria in the medical facility by at least 70% on a sampling surface; and maintaining the reduced level for at least 30 days. In one embodiment, the treatment area provides medical services to high-risk patients, including patients selected from a group consisting of immunocompromised patients, neonatal patients, colonized but uninfected patients, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients. In one embodiment of the method for reducing hospital-acquired infections, the DHP gas concentration is maintained between 5.0 and 40 ppb. In other embodiments, the DHP gas concentration is maintained between 5.0 and 30 ppb.

[0078] This disclosure provides, and includes, a method for reducing hospital-acquired infections by at least 25% when DHP is maintained at a concentration between 5.0 and 50 ppb, measured over a three-month window. In one embodiment of the method for reducing hospital-acquired infections, the DHP gas concentration is maintained between 5.0 and 40 ppb. In another embodiment, the DHP gas concentration is maintained between 5.0 and 30 ppb. In some embodiments, the number of hospital-acquired infections is reduced for high-risk patients selected from a group consisting of immunocompromised patients, neonatal patients, colonized but uninfected patients, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients. In some embodiments, the reduction in microbial load provides at least a 30%, 40%, 50%, or greater reduction in hospital-acquired infections. In some embodiments, hospital-acquired infections are virtually eliminated compared to past levels.

[0079] This disclosure provides, and includes, a method for reducing monthly reportable infections by at least 10% when measured over a three-month window in an environment maintained at a DHP concentration between 1.0 ppb and 50 ppb. In some embodiments, the number of monthly reportable infections is reduced for high-risk patients selected from a group consisting of immunocompromised patients, neonatal patients, colonized but uninfected patients, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients. In some embodiments, the reduction in microbial load provides a reduction of at least 30%, 40%, 50%, or more in monthly reportable infections. In some embodiments, monthly reportable infections are significantly reduced compared to historical levels. In embodiments of the method for reducing reportable infections, the DHP gas concentration is maintained between 5.0 and 40 ppb. In other embodiments, the DHP gas concentration is maintained between 5.0 and 30 ppb.

[0080] This disclosure provides, and includes, a method for reducing the standardized infection ratio by at least 10% when measured over a three-month window. In some embodiments, the standardized infection ratio is reduced for high-risk patients selected from a group consisting of immunocompromised patients, neonatal patients, colonized but uninfected patients, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients. In some embodiments, the reduction in microbial load provides a reduction in the standardized infection ratio of at least 30%, 40%, 50%, or more. In some embodiments, the standardized infection ratio is significantly reduced compared to past levels. In embodiments of the method for reducing the standardized infection ratio, the DHP gas concentration is maintained between 5.0 and 40 ppb. In other embodiments, the DHP gas concentration is maintained between 5.0 and 30 ppb.

[0081] This specification provides, and includes, a method for reducing the number of chain infections in a medical treatment area. A chain infection is a subset of HAI infections traceable to a primary source, consisting of a single strain of pathogenic bacteria. The method for reducing the number of chain infections includes identifying the chain infections, providing DHP at a continuous concentration from at least 5.0 ppb to a maximum of 50 ppm, and maintaining the DHP concentration until the number of chain infections decreases. In embodiments where DHP is already provided, the method further includes increasing the minimum level of DHP to at least 10 ppb. In other embodiments, the DHP level is increased to at least 20 ppb to reduce the chain infections. The method for reducing chain infections is typically implemented as part of a comprehensive preventive and proactive effort, including isotyping of the infectious agent. In embodiments of the method for reducing the number of chain infections, the DHP gas concentration is maintained between 5.0 and 40 ppb. In other embodiments, the DHP gas concentration is maintained between 5.0 and 30 ppb.

[0082] Methods for reducing the number of associated chain infections in a medical treatment area include providing one or more dry hydrogen peroxide (DHP) generators for 110 and 185 square meters and for total volumes between 275 and 460 cubic meters, respectively, and maintaining the medical treatment area at an average concentration of at least 5.0 parts per billion. In a further embodiment, the DHP gas concentration is maintained between 5.0 and 30 ppb. In an embodiment, the DHP concentration is less than 60 ppb. In an embodiment according to this disclosure, the medical treatment area is a climate-controlled treatment area maintained at a humidity between 30 and 60% and a temperature between 68°C and 73°C within a critical area (e.g., ICU, operating room, burn treatment area). See EC.02.06.01, EP 13. In an embodiment, the treatment area is maintained at a humidity between 20 and 70% and a temperature between 68°C and 73°C within a critical area (e.g., ICU, operating room, burn treatment area).

[0083] This specification provides, and includes, a method for reducing the number of chain infections by at least 25% compared to past levels. In one embodiment, the number of chain infections is at least 25% compared to a period of at least three months prior without DHP. In another embodiment, the number of chain infections is reduced between 25% and 50% compared to a state without DHP treatment.

[0084] This specification provides, and includes, methods for reducing secondary spread of infection in a healthcare setting. Methods for reducing secondary spread of infection include identifying a primary infection requiring prevention of secondary infection, providing DHP at a continuous concentration of at least 5.0 ppb, and maintaining the DHP concentration until the threat of secondary spread of infection is reduced. In embodiments, DHP is provided continuously between 10 and 20 ppb. In embodiments where DHP has already been provided, the method further includes increasing the minimum level of DHP to at least 10 ppb. In other embodiments, the DHP level is increased to at least 20, 30, or 40 ppb to reduce secondary spread of infection. Methods for reducing secondary spread of infection are typically implemented as part of a comprehensive preventive and proactive approach, including isotyping of infectious agents.

[0085] Methods for reducing the secondary spread of related infections in a medical treatment area include providing one or more DHP generators for each of 110 and 185 square meters and for total volumes between 275 and 460 cubic meters, respectively, and maintaining the medical treatment area at an average concentration of at least 5.0 parts per billion. In a further embodiment, the DHP gas concentration is maintained between 5.0 and 50 ppb. In an embodiment, the DHP concentration is less than 60 ppb. In an embodiment according to this disclosure, the medical treatment area is a climate-controlled treatment area maintained at a humidity between 20 and 60% and a temperature between 68°C and 73°C. See EC.02.06.01, EP 13. In an embodiment according to this disclosure, reducing the secondary spread of infection includes adding one or more additional DHP generating units to increase the total DHP concentration to a desired level above 20 ppb. In one embodiment, the method provides a “crush cart” comprising one or more additional DHP devices that can be added to a site to temporarily increase the local concentration of DHP to 30, 40, or 50 ppb, and maintain the higher DHP concentration for one, two, or three weeks, or until the threat of secondary infection subsides.

[0086] This specification provides, and includes, methods for reducing multidrug-resistant organisms (MDROs) on the surface of a medical treatment area. Methods for reducing MDROs can be implemented as part of a comprehensive preventive proactive effort, or can be provided to treatment areas where infection reduction is required after detection of high infection rates or an increase in infection rates.

[0087] Methods for reducing MDRO in a medical treatment area include providing one or more DHP generators for each area between 110 and 185 square meters and for total volumes between 275 and 460 cubic meters; maintaining the medical treatment area at an average concentration of at least 5.0 parts per billion and a maximum of 50 ppb, distributing this over 90% of the treatment area's volume for at least three months; and ensuring that hospital-acquired infections in at-risk patients are reduced by at least 25% as measured over a three-month window. In some embodiments, the DHP gas concentration is maintained between 5.0 and 30 ppb. In some embodiments, the DHP concentration is less than 60 ppb. In some embodiments of this disclosure, the medical treatment area is an air-conditioned treatment area maintained at a humidity between 20 and 60% and a temperature between 68°C and 73°C. See EC.02.06.01, EP 13.

[0088] In one embodiment, a method for reducing MDRO on the surface of a medical treatment area includes reducing the MDRO load on the sampling surface by at least 70% compared to an untreated treatment area of ​​similar size and use. In another embodiment, the MDRO load is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period.

[0089] In one embodiment, a method for reducing MDRO on a surface in a medical treatment area includes reducing the number of MDRO-positive sampling surfaces by at least 70% compared to an untreated treatment area. In another embodiment, the number of MDRO-positive sampling surfaces is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In another embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing MDRO-positive sampling surfaces in a medical facility by at least 70% on the sampling surface and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing MDRO-positive sampling surfaces in a medical facility by at least 80% on the sampling surface and maintaining the reduced level for at least 30 days. In yet another embodiment, MDRO-positive sampling surfaces are reduced by at least 90%. In yet another embodiment, bacteria are reduced by 95%, 99.0%, or 99.9%. In yet another embodiment, the method measures 1 square centimeter (cm) on a high-frequency contact surface. 2 Reduce MDRO levels to less than 5 colony-forming units (cfu) per unit.

[0090] In one embodiment, a method for reducing MDRO on the surface of a medical treatment area includes reducing the number of colony-forming units (CFU / plate) per contact plate on a contact plate by at least 70% compared to an untreated treatment area. In another embodiment, the number of CFUs per contact plate is reduced by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. In another embodiment, the reduction is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the CFUs per contact plate by at least 70% on a sampling surface in a medical facility and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing the CFUs per contact plate by at least 80% on a sampling surface in a medical facility and maintaining the reduced level for at least 30 days. In yet another embodiment, the CFUs per contact plate are reduced by at least 90%. In yet another embodiment, the CFUs per contact plate are reduced by 95%, 99.0%, or 99.9%. In one embodiment, the method involves determining the CFU level per contact plate on a high-frequency contact surface in 1 square centimeter (cm²). 2 Reduce the number of colony-forming units (CFUs) per colony to less than 5.

[0091] In one embodiment, a method for reducing MDRO includes reducing the average number of RLUs per sample by at least 70% compared to the same treatment space before DHP treatment, when measured over the same period. Methods and apparatus for measuring RLUs are well known in the art. In one embodiment, the reduction is measured and compared over a period of three months, six months, or annually. The method provides reducing the RLU per sample in a healthcare facility by at least 70% relative to the sample and maintaining the reduced level for at least 30 days. In another embodiment, the method provides reducing the RLU per sample in a healthcare facility by at least 80% relative to the sample and maintaining the reduced level for at least 30 days. In another embodiment, the RLU per sample is reduced by at least 90%. In yet another embodiment, the RLU per sampling surface is reduced by 95%, 99.0%, or 99.9%. RLU samples can be collected from surfaces or air.

[0092] In one embodiment, a method for reducing MDRO on the surface of a medical treatment area includes reducing the MDRO population within the medical facility by at least 90% on average when measured at 20 swipe sites per week over a month, and maintaining the reduced level for at least 5 weeks. In another embodiment, the reduction in MDRO is measured and compared over a period of 3 months, 6 months, or annually. The method provides reducing the MDRO per sampling surface within the medical facility by at least 95% on the sampling surface and maintaining the reduced level for at least 30 days. In yet another embodiment, the method provides reducing the MDRO per sampling surface within the medical facility by at least 99% on the sampling surface and maintaining the reduced level for at least 30 days. In yet another embodiment, the MDRO per sampling surface is reduced by 95%, 99.0%, or 99.9%. In this context, MDRO is a group of pathogenic bacteria selected from the following: vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus, multidrug-resistant Gram-negative bacteria (MDR GNB), aerobic bacterial colonies (ABC), and Clostridium difficile.

[0093] In one embodiment, the sampling surface is a high-frequency contact surface. In another embodiment, the sampling surface includes high-frequency and medium-frequency contact surfaces. In one embodiment, at least 90% of the sampling surface is a high-frequency contact surface selected from the group consisting of bed rails, head and footboard rails, tray tables, supply carts, door knobs, push plates, handles, elevator buttons, switches, keyboards, mice, touchscreens, blood pressure cuffs, privacy curtains, blinds, windowsills, furniture, and bathroom sinks. In one embodiment, 95% of the sampling surface is selected from the group consisting of bed rails, head and footboard rails, tray tables, door knobs, push plates, handles, privacy curtains, furniture, and bathroom sinks. In one embodiment, the sampling surface is selected from the group consisting of bed rails, head and footboard rails, tray tables, door knobs, push plates, handles, privacy curtains, furniture, and bathroom sinks. In one embodiment, the treatment area is an ICU, and the high-frequency contact surface is selected from the group consisting of bed rails, bed surfaces, and supply carts. In this embodiment, the treatment area is a medical / surgical bed, and the high-frequency contact surface is selected from the group consisting of bed rails, overbed tables, intravenous pumps, and bed surfaces. ru.

[0094] The disclosure further provides a method for MDRO on a privacy curtain, which includes continuously supplying DHP to a maximum of 50 ppb at a DHP concentration of at least 1.0 parts per billion, thereby reducing the level of microorganisms on the curtain by at least 99%.

[0095] In aspects of this disclosure, the treatment area for a method to reduce surface MDRO is selected from the group consisting of wards, HVAC zones, and air exchange restricted rooms. In other aspects, the treatment area for a method to reduce surface MDRO is selected from the group consisting of intensive care units (ICUs), oncology wards, nephrodialysis units, neonatal ICUs, and pediatric ICUs.

[0096] In one embodiment, a method for reducing MDRO on a surface in a medical treatment area includes: continuously operating one or more dry hydrogen peroxide (DHP) generators to generate and disperse DHP within the treatment area; establishing a DHP concentration of at least 1.0 parts per billion to a maximum of 50 ppb dispersed over at least 90% of the volume of the treatment area; maintaining DHP at a concentration of at least 5.0 parts per billion to a maximum of 50 ppb for at least one week; reducing MDRO on a sampling surface within the medical facility by at least 70%; and maintaining the reduced level for at least 30 days. In one embodiment, the treatment area provides medical services to high-risk patients, including patients selected from a group consisting of immunocompromised patients, neonatal patients, colonized but uninfected patients, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients.

[0097] As used herein, DHP comprises gaseous hydrogen peroxide (H2O2) substantially free of hydrate, ozone, plasma species, or organic species. DHP can be measured using methods known in the art. A preferred method for measuring DHP to avoid cross-reactivity with other oxidizing agents is performed using a Picarro PI2114 gas concentration analyzer (Picarro, Inc., California) to measure low hydrogen peroxide levels of 3 ppb. The Picarro instrument uses cavity ring-down spectroscopy (CRDS) to measure its intrinsic near-infrared absorption spectrum and effective path length measured in kilometers. The Picarro CRDS approach is used for superoxide (O2 - It can distinguish between closely related oxidizing molecules such as superoxide (O2). The Interscan 4000 series hydrogen peroxide (H2O2) gas analyzer can also be used (Interscan Corporation, California). Interscan instruments can distinguish between superoxide (O2) and other closely related oxidizing molecules. -Oxidizing molecules are measured using an electrochemical voltametric sensor that can cross-detect other oxidizing agents such as hydroxyl radicals (OH*), ozone (O3), and other reactive species. The presence of other oxidizing species can be observed near the source and decreases with distance. In contrast to most oxidizing molecules produced from non-thermal plasma, DHP is stable and can be detected at low levels throughout the space. Therefore, the presence of non-DHP oxidizing molecules can be inferred by comparing values ​​near the source with values ​​at a certain distance. In general, these non-DHPs contribute only a few parts per billion to the overall measurement.

[0098] As used herein, the terms “ozone-free” or “substantially ozone-free” mean an amount of ozone less than about 0.015 ppm. In one embodiment, “ozone-free” means that the amount of ozone produced by the device is less than or close to the detection level (LOD) using conventional detection means. Ozone detectors are known in the art and have a detection threshold of parts per billion using point ionization detection. A suitable ozone detector is the Honeywell Analytics Midas® gas detector.

[0099] As used herein, “hydration-free” means that hydrogen peroxide gas contains at least 99% water molecules bound by electrostatic attraction and London forces. The hydrated form of hydrogen peroxide is produced by the evaporation and atomization of aqueous hydrogen peroxide (AHP). The aerosols and vapors produced from AHP are the hydrated form of hydrogen peroxide, each molecule surrounded by a shell of water molecules (hydration shell) bound by electrostatic attraction and London forces. Various “drying” methods exist, but such methods cannot remove the hydration shell. Also as used herein, plasma species-free DHP means hydrogen peroxide gas that contains at least 99% hydroxide ions, hydroxide radicals, hydronium ions, hydrogen radicals, and combinations thereof.

[0100] As used herein, the term “approximately” refers to ±10%.

[0101] As used herein, “treatment area” refers to a separate, isolated area where air exchange with adjacent areas is restricted. In embodiments, a treatment area refers to a single zone of a heating, ventilation, and air conditioning (HVAC) system. An HVAC zone is defined as an area serviced by a single air handler, where all spaces in a discrete environment receive supply air from its dedicated single air handler, and all recycled air from that space is returned to that same air handler. This also includes isolated areas within an HVAC zone where air exchange with adjacent areas is restricted by sealing vents and returns, otherwise limiting the dilution of DHP. In embodiments, a treatment area may be a single, undivided ward, an intensive care unit (ICU), an oncology ward, a nephrodialysis unit, a neonatal ICU, or a pediatric ICU. The areas of healthcare facilities requiring DHP treatment include, but are not limited to, accredited hospitals, accredited outpatient surgical centers (LASCs), accredited mammography service centers (CMSs), accredited local health centers (CRHCs), long-term acute care (LTACs), nursing homes, and end-stage renal dialysis centers (RDCs).

[0102] The terms "comprises," "comprising," "includes," "including," and "having," as well as their conjugations, all mean "to include, but not limited to."

[0103] As used herein, the term “higher” means at least about 3%, 5%, 7%, 10%, 15%, 20%, 25%, 30%, 50%, 60%, 70%, 80%, 90%, or several times higher.

[0104] As used herein, the terms “improve” and “increase” mean an increase of at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, or more.

[0105] As used herein, the term “less” means at least about 3%, 5%, 7%, 10%, 15%, 20%, 25%, 30%, 50%, 60%, 70%, 80%, 90%, or several times lower.

[0106] As used herein, the terms “reducing” and “decreasing” mean a reduction of at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, or more.

[0107] The term "consisting of" means "including and limited to."

[0108] The term “essentially derived from” means that the composition, method, or structure may include additional components, steps, and / or parts, but only if the additional components, steps, and / or parts do not substantially alter the basic and novel features of the claimed composition, method, or structure.

[0109] As used herein, the singular forms "a," "an," and "the" include plural references unless the context clearly indicates otherwise. For example, the terms "compound" or "at least one compound" may include multiple compounds, including mixtures thereof.

[0110] Whenever a numerical range is indicated in this specification, it means that any cited number (fraction or integer) within the indicated range is included. The phrases “ranging / ranges between” and “ranging / ranges from” the first and second indicators are used interchangeably in this specification and mean that the first and second indicators and all fractions and integers between them are included.

[0111] As used herein, the term “method” means a set of modes, means, techniques, and procedures for accomplishing a given task, and includes, but is not limited to, modes, means, techniques, and procedures known to practitioners in the fields of agriculture, chemistry, pharmacology, biology, biochemistry, and medicine, or readily developed from known modes, means, techniques, and procedures. A method may include one or more steps.

[0112] In embodiments of this disclosure, the enclosure comprises a volume having at least one opening for air inflow and at least one opening for exhaust of air containing dry hydrogen peroxide gas. In some embodiments, the enclosure may be prepared from polyethylene, polypropylene, polystyrene, nylon, or polyvinyl chloride.

[0113] In other embodiments as used herein, the enclosure may include a heating, ventilation, and air conditioning (HVAC) system. In other embodiments, the apparatus for producing DHP is an apparatus that is installed in an HVAC system during construction. Appropriate HVAC systems and appropriate standards, e.g., standards developed by Sheet Metal & Air Conditioning Contractors' National Association (SMACNA), are known in the art. Apparatus suitable for installation in an HVAC system, as provided herein, includes the elements enumerated for standalone apparatus, but the enclosure and air distribution system are provided by the HVAC system. Apparatus suitable for installation in an HVAC system may further comprise an additional air distribution system (e.g., separate from the air distribution system of the HVAC system as a whole). Apparatus suitable for installation in an HVAC system may further comprise one or more additional filters to prevent contamination by dust or chemicals.

[0114] As used herein, a soft surface is a porous, flexible surface typically made of fabric, including, but not limited to, privacy curtains, blinds, furniture, sheets, and clothing. Soft surfaces are a significant source of microorganisms and are difficult to clean and disinfect.

[0115] While this disclosure has been described with reference to specific embodiments, those skilled in the art will understand that various modifications can be made and elements can be replaced with equivalents without departing from the scope of this disclosure. Furthermore, many modifications can be made without departing from the scope of this disclosure to adapt specific circumstances or materials to the teachings of this disclosure.

[0116] Therefore, this disclosure is not limited to any particular embodiment disclosed as the best form intended for carrying out this disclosure, and this disclosure is intended to include all embodiments that fall within the scope of the appended claims and spirit.

[0117] Embodiment Embodiment 1: Installing at least one catalyst sail-dried hydrogen peroxide (DHP) generator having a new, unused catalyst sail into the environment for microbial reduction, The operation of at least one DHP generator to generate and distribute DHP, The aforementioned environment is adjusted using DHP, and adjustment time is provided for monitoring VOC and DHP concentrations. The catalyst sail is replaced, and a DHP concentration between 1 and 50 parts per billion (ppb) is established in the environment. Maintain DHP at a concentration between 1 and 50 ppb for at least one week, A method for establishing a DHP-containing environment for microbial reduction, including the above.

[0118] Embodiment 2: The method according to Embodiment 1, wherein the adjustment time is at least one week, or the time required for the VOC concentration to fall below zero when measured using an instrument having a sensitivity of 0.5 ppm. Embodiment 3: The method according to Embodiment 1 or 2, wherein the environment comprises at least one DHP generator every 110 square meters, every 185 square meters, and every total volume between 275 and 460 cubic meters. Embodiment 4: The method according to any one of Embodiments 1 to 3, wherein the protectable space is isolated from the surrounding space and the HVAC system.

[0119] Embodiment 5: The method according to any one of Embodiments 1 to 4, wherein the protectable space includes a single zone of the HVAC system. Embodiment 6: The method according to Embodiment 5, comprising at least three DHP generators, with one DHP generator every 110 square meters, every 185 square meters, and every volume between 275 and 460 cubic meters. Embodiment 7: The method according to Embodiment 6, wherein each of the at least three DHP generators is installed within 20 meters of the second DHP generator and is arranged to provide overlapping coverage zones.

[0120] Embodiment 8: A method for reducing hospital-acquired infections in a medical procedure area requiring a method for reducing hospital-acquired infections, This involves continuously operating one or more DHP generators within the treatment area where they are needed, To establish an average DHP concentration of at least 1.0 ppb and to disperse it over at least 90% of the volume of the treatment area, Maintain DHP at an average concentration of at least 5.0 parts per billion to a maximum of 40 ppb for at least one week, A method comprising reducing pathogenic bacteria within the aforementioned medical facility.

[0121] Embodiment 9: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, wherein the risk of hospital-acquired infection in patients at risk is reduced by at least 25% as measured over a three-month window. Embodiment 9b: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, wherein the hospital-acquired infection rate in patients at risk is reduced by at least 25% as measured over a three-month window. Embodiment 9c: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, thereby reducing the number of chain infections by at least 25%. Embodiment 9d: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, thereby reducing the secondary spread of infection by at least 25%. Embodiment 9e: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, wherein the average of three monthly reportable infections is reduced compared to the untreated three months. Embodiment 9f: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, wherein the standardized infection ratio (SIR) decreases. Embodiment 9g: The method according to Embodiment 8, further comprising operating the DHP generator continuously for at least three months, wherein the treatment area includes a ward, an HVAC zone, and an air exchange restriction room.

[0122] Embodiment 10: The method according to Embodiment 8 or 9, wherein the medical treatment area is located within a long-term care facility selected from the group consisting of nursing care facilities and advanced nursing facilities. Embodiment 11: The method according to any one of Embodiments 8 to 10, wherein the pathogenic bacteria are selected from the group consisting of vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus, multidrug-resistant Gram-negative bacteria (MDR GNB), aerobic bacterial colonies (ABC), and Clostridium difficile.

[0123] Embodiment 12a: The method according to any one of Embodiments 8 to 11, wherein the reduction is to reduce the number of microorganisms on the sampling surface by at least 70% and maintain the reduced level for at least 30 days. Embodiment 12b: The method according to any one of Embodiments 8 to 11, wherein the reduction is a reduction of at least 70% in the number of colonies on the sampling surface compared to an untreated area of ​​similar size and use. Embodiment 12c: The method according to any one of Embodiments 8 to 11, wherein the reduction is a reduction of at least 70% in the number of MRSA-positive sampling surfaces compared to the untreated area. Embodiment 12d: The method according to any one of Embodiments 8 to 11, wherein the reduction is a reduction of at least 70% in the number of ABC-positive sampling surfaces compared to the untreated area. Embodiment 12e: The method according to any one of Embodiments 8 to 11, wherein the reduction is a reduction of at least 70% in the number of C.diff-positive sampling surfaces compared to the untreated area. Embodiment 12f: The method according to any one of Embodiments 8 to 11, wherein the reduction is a reduction of at least 70% in the number of colony-forming units per plate (CFU / plate) on the contact plate compared to the untreated area. Embodiment 12g: The method according to any one of Embodiments 8 to 11, wherein the reduction reduces the pathogenic bacterial population in the medical facility by at least 90% when measured at swipe sites 20 times a week for one month, and maintains the reduced level for at least 5 weeks, and Embodiment 12h: The method according to any one of Embodiments 8 to 11, wherein the reduction reduces central line-associated bloodstream infections (CLABSI) by at least 25%.

[0124] Embodiment 13: The method according to any one of Embodiments 8 to 12, wherein the sampling surface includes a high-frequency contact surface selected from the group consisting of bed rails, head and foot rails, tray tables, door knobs, push plates, handles, elevator buttons, switches, keyboards, mice, touchscreens, blood pressure cuffs, privacy curtains, blinds, windowsills, furniture, and bathroom sinks. Embodiment 13: The method according to any one of Embodiments 8 to 12, wherein the sampling surface includes a high-frequency contact surface selected from the group consisting of bed rails, head and foot rails, tray tables, door knobs, push plates, handles, elevator buttons, switches, keyboards, mice, touchscreens, blood pressure cuffs, privacy curtains, blinds, windowsills, furniture, and bathroom sinks.

[0125] Embodiment 14a: The method according to any one of Embodiments 8 to 13, wherein pathogenic bacteria are reduced by at least 80%. Embodiment 14b: The method according to any one of Embodiments 8 to 13, wherein pathogenic bacteria are reduced by at least 90%. Embodiment 14c: The method according to any one of Embodiments 8 to 13, wherein pathogenic bacteria are reduced by at least 95%. Embodiment 14d: The method according to any one of Embodiments 8 to 13, wherein pathogenic bacteria are reduced by at least 99%. Embodiment 14e: The method according to any one of Embodiments 8 to 13, wherein pathogenic bacteria are reduced by at least 99.9%.

[0126] Embodiment 15: The method according to Embodiment 8, wherein the treatment area is an open ward, an intensive care unit (ICU), an oncology ward, a renal dialysis unit, a neonatal ICU, or a pediatric ICU. Embodiment 16: The method according to any one of Embodiments 8 to 15, wherein the medical facility is an authorized hospital, an authorized outpatient surgical center, an accredited mammography service center, an accredited local health clinic, or an end-stage renal dialysis center. Embodiment 17: The method according to any one of Embodiments 8 to 16, wherein the treatment area includes a single zone of a heating, ventilation, and air conditioning (HVAC) system. Embodiment 18: The method according to any one of Embodiments 8 to 17, wherein the treatment area is maintained at a relative humidity between 20 and 70%. Embodiment 19: The method according to any one of Embodiments 8 to 18, wherein the DHP concentration is maintained between 5 and 20 ppb. Embodiment 20: The method according to any one of Embodiments 8 to 19, wherein the patients at risk are selected from the group consisting of immunocompromised patients, neonatal patients, patients with colonization but who are not infected, patients occupying rooms recently vacated by MDRO-positive patients, and renal dialysis patients.

[0127] Embodiment 21: A method for reducing multidrug-resistant organisms (MDROs) on the surface of a medical treatment area, One or more dry hydrogen peroxide (DHP) generators are operated continuously to generate and disperse DHP within the treatment area. Establish a DHP concentration of at least 5.0 ppb and disperse it within the treatment area. Maintain DHP at a concentration between 5.0 and 50 ppb for at least one week, A method comprising reducing the number of multidrug-resistant organisms (MDROs) in the medical facility by at least 70% and maintaining the reduced level for at least 30 days. Embodiment 22: The method according to Embodiment 21, further comprising reducing hospital-acquired infections by at least 25% when measured within a 3-month window. Embodiment 23: The method according to Embodiment 21 or 22, wherein the medical facility requiring the method is subject to reintroduction and contamination of multidrug-resistant organisms (MDROs). Embodiment 24: The method according to Embodiment 21, 22, or 23, wherein the dispersion covers at least 90% of the entire volume of the treatment area.

[0128] Embodiment 25: A method for reducing the spread of infection in an acute infectious outbreak in a medical treatment area requiring a method for reducing the spread of infection in an acute infectious outbreak, Identifying treatment areas with acute infection outbreaks, To provide one or more dry hydrogen peroxide (DHP) generators for generating and dispersing DHP within the aforementioned treatment area, To establish DHP concentrations between 1.0 and 50 parts per billion, Maintain DHP at a concentration of at least 5.0 ppb until the aforementioned acute infectious outbreak is brought under control. Methods that include... Embodiment 26: The method of Embodiment 25, wherein the treatment area has not been previously treated with DHP, is not currently treated with DHP, or has an average DHP concentration of less than 5.0 ppb in the treatment area. Embodiment 27: The method according to Embodiment 26, wherein the treatment area is a DHP-containing treatment area, and the provision comprises providing one or more standalone DHP generating units.

[0129] Examples Example 1: Configuration of the DHP generation unit To determine the required number and placement of units, the target space is evaluated before the installation of the DHP generating units. Variables include HVAC system characteristics such as the number of vents and return vents, air turnover, humidity, and temperature.

[0130] Example 2: Community Hospital Cardiovascular / Telemetry Study In this example, DHP is integrated into the existing heating, ventilation, and air conditioning (HVAC) system of a community hospital within a 34-bed cardiovascular / telemetry (CV-Tele or CVTL) unit. This study is designed to answer two fundamental and important questions: Does DHP reduce microbial contamination in the hospital unit? Can DHP reduce the observed rate of HAI? This study consists of three phases. Phase 1 involves taking culture samples from patient rooms and common work areas before and after standard disinfection of rooms to establish a baseline of microbial colonization before applying the DHP technology. Phase 2 involves taking samples daily from the same rooms and work areas for one week after the introduction of the DHP technology. Phase 3 involves observing the HAI rate in the CV-Tele unit over a six-month period and comparing it to the same six-month window that occurred in the previous year.

[0131] The DHP technology is installed in a 34-bed cardiovascular telemetry unit (CV-Tele) in a hospital via the existing heating, ventilation, and air conditioning (HVAC) system. The DHP technology uses ultraviolet light in the UVA band at approximately 365 nanometers (nm) to activate the catalyst. This light is non-sterilizing and safe for use in occupied areas.

[0132] DHP systems can be deployed on a standalone basis or within a heating, ventilation, and air conditioning (HVAC) system. A single standalone DHP unit in current designs is sufficient to protect an isolated room with a volume of up to approximately 110 cubic meters (6 x 6 x 3 meters). In this example, all devices are mounted in the HVAC system at a ratio of one device per 1,482 square feet of treatment area, separated by no more than 20 meters, preferably no more than 15 meters. Furthermore, multiple units are installed to ensure overlapping coverage zones. This configuration is important for establishing and maintaining an appropriate DHP level of at least 20 ppb, preferably 30–50 ppb.

[0133] Stage I - Historical Analysis and Sampling Stage I establishes a baseline for comparative DHP use. Historical HAI rates by type and location are compiled into tables, and existing HAI mitigation strategies and procedures are cataloged. Microbial sampling is performed in three wards against selected target and air samples on high-contact / high-use (HTHU) surfaces. Both pre- and post-final wash samples are collected.

[0134] Stage II - Reduction of microbial contaminants Stage II evaluates the DHP technology's ability to reduce microbial contaminants in the environment. High-contact / high-use (HTHU) surfaces are cultured for bacteria and fungi before and after the final cleaning of a standard hospital, as well as at 48, 96, and 187 hours after cleaning. A total of 13 different surfaces are cultured from the same three sample rooms: patient bed rails, nurse call buttons, faucet handles, faucets, curtains, soap dispensers, toilets, toilet handles, computer keyboards, computer shelves, bathroom door handles, sinks, and windowsills.

[0135] DHP level monitoring uses multiple techniques to provide timely feedback on levels across the entire environment. During the study, steady-state DHP levels were observed in the range of 1.0 ppb to 50 ppb, well below safety limits.

[0136] Continuous sampling of DHP levels is achieved using Chemdaq Steri-Trac Area Monitors. This technology provides continuous sampling of DHP levels (0.0 ppm to 20 ppm) in a research area with data logger functionality. The LED display provides continuous readings of hydrogen peroxide levels with a resolution of up to 0.1 ppm and features a loud alarm horn that warns if levels exceed 1.0 ppm. A remote repeater provides central monitoring (nurse station) for the system used. Preferably, an Interscan 4000 series hydrogen peroxide (H2O2) gas analyzer can be used (Interscan Corporation, California). Interscan 4000 series instruments such as the 4090-1999b can detect a range between 0 and 1999 ppb with a resolution of 1 ppb. Even more preferably, DHP can be measured using a Picarro PI2114 gas concentration analyzer (Picarro, California, Inc.) which can measure hydrogen peroxide levels as low as 3 ppb.

[0137] The Draeger X-am 5100 provides continuous sampling of ambient hydrogen peroxide levels and is designed as a wearable personal protective monitor. This device offers continuous sampling of DHP levels (0.5 ppm to 20 ppm), can be worn by staff, and has data logging capabilities. The intuitive LED display provides continuous readings of hydrogen peroxide levels with a resolution of up to 0.1 ppm and features a loud alarm horn that warns of levels exceeding 1.0 ppm.

[0138] Three Steri-Trac monitors with remotes cover representative samples of the treated area. An X-am 5100 personal monitor is used to obtain additional periodic samples of the treated area. Combined, this sampling method provides fixed, mobile, continuous sampling of the entire treated area, effectively mitigating the risk of exceeding the exposure threshold. Levels exceeding the safety threshold of 1.0 ppm were not observed during the study period.

[0139] The sampling performed in stages I and II is shown in Table 1 below.

[0140] [Table 1]

[0141] Stage III - Reduction of healthcare-associated infections Stage III assesses the DHP technology's ability to reduce HAI over time. The HAI rate observed in the CV-Tele study area over a six-month period is compared to the rate observed during the same six-month window in the previous year. The number and type of infections, as well as the causative pathogens, are recorded.

[0142] After performing DHP technology in the CV-Tele unit, a significant reduction in microbial colony formation was observed over a 7-day period. Complete eradication of Staphylococcus aureus, Candida parapsis, Pseudomonas putida, Flavobacterium meningosepticum, Pseudomonas piketi, and Citrobacter was observed on day 7. A reduction in the number of Alcalagine colonies by 68%, Pseudomonas aeruginosa by 95%, and Enterobacter by 50% was observed from the final wash up to 7 days later. The culture results are shown in Table 2 below.

[0143] A decrease in the infection rate occurring in the CV-Tele unit is observed during the study period compared to the same 6-month period of the previous year. A total of 3 infections were observed during the study period compared to 8 infections in the previous year. This represents a 62.5% decrease in the observed infection rate. Calculated per patient hospitalization day, the infection rate decreased from 0.18 infections / day to 0.07 infections / day. During the study period, one case of gastrointestinal infection caused by Escherischia coli, one case of gastrointestinal infection caused by Clostridium difficile, and one case of pneumonia caused by Escherischia coli and Haemophilus influenzae were observed. Monthly infection rates are provided in Table 3. Infection rate plots are shown in Figure 1.

[0144] [Table 2]

[0145] [Table 3]

[0146] Example 3: VA Hospital Community Residential Center Sampling High-frequency contact surfaces in both the treatment and control areas are identified, and an equal number of equivalent locations are selected for the study. Specific rooms for the investigation are selected for convenience, as well as by new admissions, regardless of the presence or absence of MRSA and / or C. diff infection recorded at admission. This approach has been validated in previous studies on UV and is consistent with real-world hospital environments. Importantly, the installation and testing of DHP units are carried out without interruption or modification of existing antimicrobial and infection reduction protocols and methods.

[0147] Microbial reduction is tested by counting aerobic bacterial colonies (ABC), MRSA, and C. diff n surface samples taken from intervention and control rooms. For both control and DHP test areas, each of the 10 rooms is sampled on five high-frequency contact surfaces, including bed rails on both sides of the bed (surfaces #1 and #2), head and foot rails (surface #3), tray table (surface #4), and bathroom sink (surface #5). Baseline measurements are collected weekly for each of the five selected surfaces over 12 weeks. After DHP installation, there are 10 intervention rooms (samples per room type - ABC, MRSA, C. diff) and 10 (10) control rooms.

[0148] Samples are collected using Rodac press plates utilizing the roll plate method. TSA plates containing Tween 80, a special selective medium in Rodac plates, are used for MRSA and C. diff. Samples are collected from week 3(3) to week 12(12) of each study period. A two-week delay is given before sampling to normalize the adjustment phase and room atmospheric conditions before or after DHP installation (for example, depending on the environment, existing "sinks" such as VOCs react with the generated DHP, so it takes time for DHP levels to stabilize).

[0149] Example 4: Research Design a. Data collection A randomized crossover study design will be used to determine bioburden levels on high-frequency contact surfaces in the presence of continuous disinfectant (DHP) in healthcare facilities at community living centers. Current intermittent strategies to reduce HAI, such as washing, handwashing, and other interventions, are expected to proceed without interruption.

[0150] Four study phases are provided. First, a 14-week pre-implantation period is conducted to assess baseline bioburden levels in two areas of the facility (area A and area B). In the second phase, one of the two areas is randomly assigned for DHP device implantation, while the second area serves as a reference. The second phase lasts 14 weeks. The third phase is a 14-week "washout period" in which neither area A nor area B receives DHP treatment. In the final phase, the treated and untreated areas are switched to conduct further testing of the DHP-treated and control areas.

[0151] The total number of samples collected is shown in Table 1.

[0152] [Table 4]

[0153] b. Data Analysis A mixed-effects regression model is used to detect the difference in ABC counts and MRSA sample counts in the treatment room compared to the control room during the post-intervention period. The regression model is specified as follows: Outcome ~ β0 + β1* group + β2* period + β3* group * period + Uiγi

[0154] In the formula, group = treatment group or control group, period = before or after intervention, and Uiγi is a random intercept from the room. Random intercepts are used to account for repeated sampling from the same room. The parameter estimate of the interaction effect (β3) shows the difference in outcomes between the treatment group and the control group during the later stages of the intervention. For ABC, a negative binomial mixed regression model is used to model the number of colonies. For MRSA, a logistic mixed regression model is used to model the number of samples with and without MRSA. All analyses are performed in R version 3.5.1.

[0155] c. Output analysis Output analysis of the mixed-effects model is performed via simulation in R version 3.5.1 using the packages "simstudy", "glmmADMB", and "lme4". For ABC, a negative binomial variance with a mean of 68 and a variance parameter of 1.5 is used. Using the variance, the control and pre-intervention ABC numbers are modeled assuming a 50% reduction in the post-intervention treatment group. 10(10) treatment rooms and 10(10) control rooms with 12(12) pre-intervention samples and 12(12) post-intervention samples (480 samples per surface) for each room for each surface give 85% output to detect this reduction, assuming that the 50% reduction in ABC numbers occurs within the first sample after the installation of the HP system.

[0156] In the case of MRSA, it is assumed that 14% of the samples in the control room and pre-intervention room will detect MRSA based on other studies. Assuming a 50% reduction in the post-intervention control room, and combining 12 pre-intervention and 12 post-intervention samples (480 samples total) from 5 surfaces in each of the 10(10) treatment rooms and 10(10) control rooms (480*5 = 2,400 samples total), and assuming this reduction occurs within the first sample after the installation of the DHP system, an 83% output is obtained to detect a 50% reduction in MRSA samples.

[0157] Example 5: Application of DHP to reduce microbial bioburden in active healthcare settings a. Samples and settings Between March and May 2019, a study approved by the facility's review committee will be conducted at a 762-bed acute care facility. A total of five units within the facility will be studied: a 24-bed pediatric intensive care unit (PICU), a 22-bed pediatric emergency department (Peds ED) unit, a 23-bed adult oncology services (AOS) unit, a 22-bed adult intensive care unit - cardiovascular trauma unit (CVTU), and a 10-bed adult trauma surgical intensive care unit (TSICU). DHP units (Synexis® BLADE, Lenexa, KS) will be installed by Synexis at the diffuser level in the HVAC system at each intervention site. The units will utilize ambient humidity and oxygen moving through the HVAC system, along with a patented plasma separation process, to generate hydrogen peroxide in a non-aqueous dry gas form ranging from 5 to 25 parts per billion (ppb) at high transient concentrations of up to 40 ppb. The DHP unit was operated continuously (24 hours a day, 7 days a week) throughout the study period in four intervention units. The fifth intervention unit (TSICU) did not have the system directly installed at its location, but HVAC was shared between the TSICU and CVTU to allow exposure to DHP.

[0158] The effectiveness of the DHP system is evaluated by comparing baseline surface microbial sampling before DHP implementation (Study Days 3, 2, and 1) with sampling after implementation (Study Days 1, 7, and 28). All microbial sampling (Table 4) is performed by Controlled Environmental Management, Fountain Hills, AZ, after standard hospital manual cleaning is performed at each location. No other changes are made to the hospital's environmental cleaning and disinfection protocols or practices during the study period.

[0159] [Table 5]

[0160] Surface samples were wiped vigorously from curtains, bed rails, and countertops using a pre-moistened blue cap swab, in a horizontal and then vertical direction, for a total length of 25 cm. 2 Samples are obtained at each location collected from the area. Curtain collection locations are at a gripping point approximately 4 feet from the ground, starting from the edge of the curtain. Bed rail samples are collected from the inside and top portion of the bed rail at a location approximately 2-3 feet from the top of the bed closest to where the call button is located. Patient room counter samples are collected only in the pediatric emergency room because there is no fixed bed at this location. Counter samples are collected at random locations on their surfaces. Final sample types are collected on adjacent cabinets in adult oncology patient units in areas that may be missed during daily cleaning. Nurse station counter samples are collected closest to where the nurse computer station on the visitor side is located.

[0161] A three-day window prior to the start of DHP was used to establish the baseline on day 0. The individual sector with the greatest reduction in microbial presence was AOS, which decreased by a total of 32 million CFU from day 0 to day 1 after implementation. Similarly, by day 28 after implementation, the reduction was equal to 32.4 million CFU. As seen in Figure 2, the overall reduction was 126.3 million CFU one day after implementation and was maintained at 120.4 million CFU on day 28 after implementation, including all units.

[0162] CFU reduction was observed in both hard and soft surfaces (Figure 3). Soft surfaces achieved the largest reduction, with a decrease of 53.6 million CFU on the first day of implementation, and maintained a reduction of 52.1 million CFU by day 28. The second highest reduction was in bed rails, with a decrease of 23.9 million CFU on day 1, which was maintained at a reduction of 24.6 million CFU by day 28.

[0163] There was a statistically significant difference in the average number of microorganisms on the surface between day 0 and day 1 (t 43.324=9.396, p<.001). The average microbial count after day 1 was 3.32 million CFU lower than the count on day 0. By day 28, the average microbial count remained statistically significant (t 39.843 (=9.165, p<.001). The average microbial count on day 28 was 3.16 million CFU lower than the count on day 0.

[0164] Table 5 lists the dominant organisms recovered from the sampled surface, recording the recovery of normal skin microbiota and potential microbial pathogens. DHP was effective in reducing the dominant Gram-negative bacilli over all three periods. Acinetobacter ruofii was found as a primary organism in fewer samples on day 7. The remaining samples did not show any change in the primary organism rank on the sample surface.

[0165] [Table 6]

[0166] b. Air sample All air sampling is performed using bioaerosol sampling pumps (Buck Bio-Culture®, APBuck Inc, Orlando, Florida) each with a total sample volume of 500 liters. A total of 48 air samples are collected from the center of each nursing station in each patient care unit. Additional samples are collected in the adult oncology corridor outside the pediatric emergency room corridor, and the final sample is collected as a baseline at the main emergency room entrance in the emergency bay. Non-nursing air samples are selected to determine the comparison between locations where DHPs are not attached and locations where DHPs are attached. Each sample consists of two sedimentation plates, one containing triptycase soybean agar (TSA) and the other containing inhibitory mold agar (IMA).

[0167] There was a statistically significant difference in the average number of microbial air samples (t 13(=2.704, p=.018). The average microbial count on day 1 was 26,800 CFU lower than the CFU count on day 0. By day 7, there was no statistically significant difference in the average microbial count (t 13 (=1.563, p=0.142). The average microbial count on day 7 was 18,600 CFU lower than the CFU count on day 0. By day 28, there was again a statistically significant difference in the average microbial count (t 13 (=2.434, p=0.030). The average microbial count on day 28 was 25,200 CFU lower than the CFU count on day 0.

[0168] Organisms are identified from the samples and ranked by growth rate for each sample. Primary organisms are ranked as those with the highest growth rate in the CFU. The top five identified airborne organisms are listed in Table 6, representing normal skin microbiota, bacteria, and pathogens. DHP results in a decrease in the identification of primary organisms such as Gram-negative bacilli, Micrococcus, and Bacillus on day 1. By day 7, a decrease in Enterococcus faecalis and Coagulase Negative Staph is observed. In particular, the levels of all organisms are significantly reduced from millions of baseline numbers. No pattern emerges among the organisms ranked number 1 in each site. On day 7, although counts were much lower, the low counts of several hundred are the highest in many sample sites compared to baseline, as Micrococcus is very common. Thus, while Micrococcus (and other organisms) are significantly reduced, its widespread presence makes Micrococcus the most common surviving organism.

[0169] [Table 7]

[0170] c. Specimen processing All samples are transported to USMicro Solutions, Inc. via overnight transport, ensuring temperature maintenance by using insulated, cryogenically packaged transport boxes. Surface samples are seeded onto blood agar plates and incubated at 20–25°C for 5 days, followed by determination of the total colony-forming unit (CFU) count. Species identification is performed based on selected isolates and is identified for epidemiologically important organisms. Air sampling is completed with TSA incubated at 20–25°C for 5 days. Species identification of Staphylococcus aureus, Enterococcus, and Gram-negative bacilli is completed using MALDI-TOF mass spectrometry, along with genus identification for all other organisms.

[0171] [Table 8] JPEG0007867006000009.jpg177145

[0172] d.Statistical analysis All surface and air sample CFU data are analyzed using paired t-tests. For all data, the mean of the baseline surface sample (Study Day 3, Day 2, Day 1) is used as a comparison point for the analysis. Statistical analysis is performed using IBM SPSS Statistics version 25. A p-value of 0.05 or less is considered statistically significant.

[0173] Bacterial count results were matched and organized by location and hospital ward, and the median baseline count for each sample location was found (Error! Reference source not found). Logarithmic decline was then observed, defined as a decrease of 10 orders of magnitude in bacterial count (i.e., 10,000 CFU to 10,000 CFU is a 1 logarithmic decline). The overall mean baseline count, as well as the overall median baseline count, exceeded 1,000,000 colony-forming units (CFU). This number was consistent with 3 of the 4 hospital units, with the sole exception being the pediatric intensive care unit (PICU), which had a median baseline count of 670,000 CFU over 3 days of the pre-intervention study across 7 locations within the unit. The median overall number decreased until day 7 after installation (>122,028 CFU), then slightly increased on day 28 (>269,319 CFU), before decreasing to its lowest level on day 60 (33,173 CFU), resulting in a 2-log decrease from the baseline median. The unit achieving the largest decrease compared to the baseline median was Adult Oncology Services, which decreased from over 1,000,000 CFU to 4,586 CFU in 60 days, a 3-log decrease. The decrease was also observed based on the type of location sampled. Two of the four nurse stations sampled achieved at least a 5-log decrease rate in 60 days, while the other two achieved 4-log and 2-log decreases. 75% (9 / 12) of the curtains tested achieved at least a 3-log decrease after 60 days, and 66.67% (8 / 12) of the hard surfaces (bed rails / counters) tested also achieved at least a 3-log decrease.

[0174] Bacterial counts underwent a natural logarithmic transformation, and a multiple linear regression model was constructed using the natural logarithm of the count as the dependent variable (Figure 2). The median baseline count is shown as day 0 to account for the baseline count before intervention, in order to eliminate the effect of high variance in baseline counts on the regression model. The main independent variable of interest in the model is time, measured in days. Covariates included in the model and controlled for include hospital unit and location type, both as categorical variables. Time was found to be a significant predictor of bacterial count with a p-value of 0.00011 (Table 9), indicating that the activation of dry hydrogen peroxide significantly affected the levels of bioburden in the four hospital units where it was installed over a two-month study. Qualitative descriptions from staff nurses included the observation that in the pediatric intensive care unit (PICU), the smell of gasoline from the helicopter pad directly above the roof was routinely noticeable before the installation of the DHP, but undetectable after installation. This indicates that DHP can decompose airborne volatile organic compounds (VOCs) in real-world occupied environments. During the study, there were no patient complaints in the treatment room that were consistent with symptoms commonly associated with excessive exposure to hydrogen peroxide, including irritation of the eyes, nose, and throat.

[0175] Another pattern is observed during the comparison and subsequent analysis of data regarding the type of surface being swabbed and the rate of reduction observed within 1 day of DHP system activation. Analysis of the rate of reduction on soft surfaces in patient rooms (curtains) compared to hard surfaces (counters / bed rails) revealed that 67% (8 / 12) of sampled curtains experienced at least a 3-log reduction in microbial load compared to 33% (4 / 12) of hard surfaces in the same patient rooms. This discrepancy is noted only on the first day after installation, but is included in subsequent analyses.

[0176] [Table 9]

[0177] [Table 10]

[0178] DHP resulted in an overall 96.5% reduction in microbial load across all areas and sampled surfaces, from 130.92 million CFU on day 0 to 4.65 million CFU on day 1. However, the greatest reduction in microbial load was observed on the surfaces most difficult to maintain cleanliness, with the curtains recording a 99.5% reduction from 53.93 million CFU on day 0 to 290,000 CFU on day 1. No increase in staff cleaning or housekeeping was implemented, nor were the curtains changed during this period.

[0179] Example 6: Application of DHP to reduce hospital-acquired infections in an active healthcare setting. Healthcare-associated infections (HAIs) are routinely monitored in active healthcare settings. HAI data from the acute care facility in Example 5 was monitored after the installation of a DHP generator and compared with data from the same period before and after the installation. Table 10 shows the date, patient age, type of infection, and date of infection discovery.

[0180] [Table 11] JPEG0007867006000013.jpg103164

[0181] Table 11 shows the distribution of infection types before and after treatment. As shown in Table 11, the total number of infections decreased, with pneumonia and gastroenteritis showing the largest decreases. Given the relatively low number of infections, whether the distribution of infections is significantly affected before and after treatment remains an unresolved question.

[0182] [Table 12]

[0183] Table 12 shows the age-based distribution of HAI. Interestingly, the number of infections in the youngest patients (under 3 years old) triples from 12 to 4. However, given the numbers, it is still unclear whether age affects HAI reduction DHP treatment.

[0184] [Table 13]

[0185] As shown in Table 13, applying DHP in an active healthcare setting without any additional modifications to standard manual washing procedures resulted in a significant reduction of over 40% in HAI infections. This reduction is remarkable and represents a substantial improvement over conventional methods, potentially leading to substantial reductions in both costs and mortality. For example, in the United States, a 40% reduction in HAI could reduce the number of deaths by nearly 40,000 and save $12 billion in costs to US hospitals. These reductions are considerably larger than other reported reductions in HAI using conventional approaches.

[0186] [Table 14]

Claims

1. A method for reducing microorganisms in a medical facility, wherein the method is: (a) Two or more dry hydrogen peroxide (DHP) generators are installed in the treatment area, at least one DHP generator is installed in and / or incorporated into the heating, ventilation and air conditioning (HVAC) system of the treatment area, and each DHP generator is equipped with an air-permeable catalyst coated mesh substrate. (b) The DHP generator is operated in a two-stage process to generate DHP molecules, and the DHP molecules are guided from the DHP generator to the treatment area, The aforementioned two-stage process is (i) An initial adjustment step for adjusting the treatment area with DHP, wherein at least one of the following three conditions (Ai) to (Ci) is satisfied during the initial adjustment step: (Ai) The relative humidity within the treatment area is at least 3% higher than the relative humidity within the treatment area measured immediately before the operation of step (b). (Bi) The level of volatile organic compounds (VOCs) in the treatment area is lower than the level of VOCs in the treatment area immediately before the operation of step (b), but is still measurable. (Ci) The concentration of DHP in the treatment area is increasing or decreasing. (ii) A maintenance step for maintaining the steady-state equilibrium concentration of the DHP within the treatment area, wherein at least one of the following three conditions (Aii) to (Cii) is satisfied during the maintenance step: (Aii) The relative humidity within the treatment area is approximately the same as the relative humidity within the treatment area measured immediately before the operation of step (b). (Bii) The level of VOCs in the treatment area is undetectable. (Cii) The concentration of DHP in the treatment area is in a steady state. A method for microbial reduction in a medical facility, comprising the following: the steady-state equilibrium concentration of the DHP in the treatment area during the maintenance phase is between 1 ppb and 50 ppb by weight over at least 90% of the volume of the treatment area in the medical facility.

2. The method according to claim 1, wherein the treatment area is provided with at least one DHP generator for every area between 110 and 185 square meters and for every volume between 275 and 460 cubic meters.

3. The method according to claim 1, wherein the treatment area is isolated from the surrounding space and the HVAC system.

4. The method according to claim 1, wherein the treatment area includes a single zone of the HVAC system.

5. The method according to claim 4, further comprising at least three of the DHP generators, wherein one DHP generator is arranged for every area between 110 and 185 square meters and for every volume between 275 and 460 cubic meters.

6. The method according to claim 5, wherein each of the at least three DHP generators is installed within 20 meters of one of the three DHP generators, and the DHP generators are arranged to provide overlapping coverage zones.

7. A method for reducing hospital-acquired infections in a medical facility that requires a method for reducing hospital-acquired infections, wherein the method is: The process includes operating two or more DHP generators in a two-step process within at least one treatment area of ​​the medical facility, wherein the operation step includes generating DHP molecules in each DHP generator and guiding the DHP molecules from the DHP generator to the treatment area. The aforementioned two-stage process is (i) An initial adjustment step for adjusting the treatment area with DHP, wherein at least one of the following three conditions (Ai) to (Ci) is satisfied during the initial adjustment step: (Ai) The relative humidity within the treatment area is at least 3% higher than the relative humidity within the treatment area measured immediately before the operation step. (Bi) The level of volatile organic compounds (VOCs) in the treatment area is lower than the level of VOCs in the treatment area immediately before the operation step. (Ci) The concentration of DHP in the treatment area is increasing or decreasing. (ii) A maintenance step for maintaining the steady-state equilibrium concentration of the DHP within the treatment area, wherein at least one of the following three conditions (Aii) to (Cii) is satisfied during the maintenance step: (Aii) The relative humidity within the treatment area is approximately the same as the relative humidity within the treatment area measured immediately before the operation step. (Bii) The level of VOCs in the treatment area is undetectable. (Cii) The concentration of DHP in the treatment area is in a steady state. The steady-state equilibrium concentration of DHP in the treatment area during the maintenance phase is between 1 ppb and 50 ppb by weight over at least 90% of the volume of the treatment area in the medical facility, thereby reducing the rate of hospital-acquired infections within the medical facility. Methods that include...

8. The maintenance step further includes the step of operating the DHP generator continuously for at least three months, The aforementioned hospital-acquired infections in patients at risk decreased by at least 25% when measured over a three-month window. The treatment area includes a ward, an HVAC zone, or an air exchange restriction room. The method according to claim 7.

9. The method according to claim 8, wherein the patient at risk is selected from one or more of the following: an immunocompromised patient, a neonatal patient, a patient who has colonized but is not infected, a patient occupying a room recently vacated by an MDRO-positive patient, or a patient undergoing renal dialysis.

10. The method according to claim 7, wherein the treatment area is located within a long-term care facility selected from a nursing care facility or an advanced nursing care facility.

11. The method according to claim 7, wherein the hospital-acquired infection is caused by a pathogen selected from one or more of the following: vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus, multidrug-resistant Gram-negative bacteria (MDR-GNB), aerobic bacterial colonies (ABC), or Clostridium difficile.

12. The method according to claim 7, wherein the maintenance reduces the number of colony-forming units (CFU / plate) per plate on the contact plate by at least 70% compared to the untreated area.

13. The method according to claim 7, wherein during the maintenance step, the microorganisms are reduced by at least 70% on the sampling surface to achieve a reduced level, and the reduced level is maintained for at least 30 days.

14. The method according to claim 13, wherein the sampling surface includes a high-frequency contact surface selected from one or more of the following: a bed rail, head and foot rail, tray table, door knob, push plate, handle, elevator button, switch, keyboard, mouse, touchscreen, blood pressure cuff, privacy curtain, blind, window sill, furniture, or bathroom sink.

15. The method according to claim 7, wherein pathogenic bacteria are reduced by at least 80%.

16. The method according to claim 7, wherein the treatment area is selected from an open ward, an intensive care unit (ICU), an oncology ward, a renal dialysis unit, a neonatal ICU, or a pediatric ICU.

17. The method according to claim 16, wherein the medical facility is selected from an authorized hospital, an authorized outpatient surgical center, an accredited mammography service center, an accredited local health clinic, or an end-stage renal dialysis center.

18. The method according to claim 7, wherein the treatment area includes a single zone of a heating, ventilation, and air conditioning (HVAC) system.

19. The method according to claim 18, wherein the treatment area is maintained at a relative humidity between 20 and 70%.

20. A method for reducing multidrug-resistant organisms (MDROs) on the surface of at least one treatment area within a medical facility, wherein the method is: The process includes a step of operating two or more dry hydrogen peroxide (DHP) generators in a two-step process to generate and disperse DHP within the treatment area, the step of generating DHP molecules in each DHP generator and guiding the DHP molecules from the DHP generators to the treatment area. The aforementioned two-stage process is (i) An initial adjustment step for adjusting the treatment area with DHP, wherein at least one of the following three conditions (Ai) to (Ci) is satisfied during the initial adjustment step: (Ai) The relative humidity within the treatment area is at least 3% higher than the relative humidity within the treatment area measured immediately before the operation step. (Bi) The level of volatile organic compounds (VOCs) in the treatment area is lower than the level of VOCs in the treatment area immediately before the operating step, but is still measurable. (Ci) The concentration of DHP in the treatment area is increasing or decreasing. (ii) A maintenance step for maintaining the steady-state equilibrium concentration of the DHP within the treatment area, wherein at least one of the following three conditions (Aii) to (Cii) is satisfied during the maintenance step: (Aii) The relative humidity within the treatment area is approximately the same as the relative humidity within the treatment area measured immediately before the operation step. (Bii) The level of VOCs in the treatment area is undetectable. (Cii) The concentration of DHP in the treatment area is in a steady state. The steady-state equilibrium concentration of DHP in the treatment area during the maintenance phase is between 1 ppb and 50 ppb by weight over at least 90% of the volume of the treatment area in the medical facility. The aforementioned maintenance stage is maintained for at least one week. The multidrug-resistant organisms (MDROs) in the medical facility are reduced by at least 70%, providing a reduced MDRO level, and the reduced MDRO level is maintained for at least 30 days. Methods that include...

21. The method according to claim 20, further comprising reducing hospital-acquired infections by at least 25% when measured within a three-month window.

22. The method according to claim 20, wherein the medical facility is subjected to reintroduction and contamination of multidrug-resistant organisms (MDROs) during the initial adjustment phase and / or the maintenance phase.

23. A method for reducing the spread of infection in an acute infectious outbreak in a healthcare facility requiring a method for reducing the spread of infection in said acute infectious outbreak, the method comprising steps (a) to (d), (a) Identify at least one treatment area having an acute infection outbreak, (b) To provide two or more dry hydrogen peroxide (DHP) generators within the treatment area, (c) Operating a dry hydrogen peroxide (DHP) generator in a two-step process to generate and disperse DHP within the treatment area, the operation of which includes generating DHP molecules in each DHP generator and guiding the DHP molecules from the DHP generator to the treatment area. The aforementioned two-stage process is (i) An initial adjustment step for adjusting the treatment area with DHP, wherein at least one of the following three conditions (Ai) to (Ci) is satisfied during the initial adjustment step: (Ai) The relative humidity within the treatment area is at least 3% higher than the relative humidity within the treatment area measured immediately before the operation of step (c). (Bi) The level of volatile organic compounds (VOCs) in the treatment area is lower than the level of VOCs in the treatment area immediately before the operation of step (c), but is still measurable. (Ci) The concentration of DHP in the treatment area is increasing or decreasing. (ii) A maintenance step for maintaining the steady-state equilibrium concentration of the DHP within the treatment area, wherein at least one of the following three conditions (Aii) to (Cii) is satisfied during the maintenance step: (Aii) The relative humidity within the treatment area is approximately the same as the relative humidity within the treatment area measured immediately before the operation of step (c). (Bii) The level of VOCs in the treatment area is undetectable. (Cii) The concentration of DHP in the treatment area is in a steady state. The steady-state equilibrium concentration of DHP in the treatment area during the maintenance phase is between 1 ppb and 50 ppb by weight over at least 90% of the volume of the treatment area in the medical facility, (d) A method for maintaining the steady-state equilibrium concentration of the DHP in the treatment area at a concentration between 1.0 and 5.0 ppb by weight over at least 90% of the volume of the treatment area in the medical facility until the acute infectious outbreak is brought under control.

24. The method according to claim 23, wherein the treatment area has not been previously treated with DHP before the operation of step (c).

25. The method according to claim 24, wherein the treatment area is a DHP-containing treatment area, and the provision includes providing one or more standalone DHP generating units.

Images