Oral composition for suppressing methane gas generation

Abstract

This invention provides an oral composition for suppressing methane gas generation. [Solution] Brown algae processing residue, sake charcoal processing residue, alcohol processing residue, vegetable oil processing residue, Lumitoleic acid and its salts, essential oil processing residues, citrus peels and their processing residues, and kale The active ingredient contains at least one selected from the group consisting of and the processing residue thereof. Prepare an oral composition for suppressing thrombocytopenia.

Description

Technical Field

[0001] The present invention relates to an oral composition for suppressing methane gas generation.

Background Art

[0002] Among the greenhouse gases emitted in Japan, the major ones are carbon dioxide, methane, nitrous dinitrogen, four gases such as alternative fluorocarbons, and hydrofluorocarbons, etc. (Non-Patent Document 1).

[0003] Among these, methane gas is released by the belches, farts, etc. of ruminant animals such as cows, sheep, and goats. The warming ability of methane gas is about 25 times that of carbon dioxide, and it is known to have a great impact on global warming. Therefore, reducing the methane gas generated from the digestive tract of ruminant animals is useful for suppressing global warming.

[0004] Means such as feeds that have an effect of suppressing methane gas have also been developed (Patent Document 1).

Prior Art Documents

Non-Patent Documents

[0005]

Non-Patent Document 1

Patent Documents

[0006]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0007] ​​It is understood that reducing methane gas produced by ruminants is useful in preventing global warming. Although this is being addressed, the development of ingredients and research to suppress it are not yet sufficient. The present invention aims to provide a composition that can suppress methane gas produced from ruminants. To target. [Means for solving the problem]

[0008] The inventors of this invention have conducted extensive research to solve this problem, and as a result, have found that brown algae processing residue and sake charcoal processing Residues, alcohol processing residues, vegetable oil processing residues, palmitoleic acid and other unsaturated fatty acids or their Salt, essential oil processing residues, citrus peels and their processing residues, and kale and its processing residues. At least one species selected from the group inhibits methane gas production in ruminants. Having discovered a practical use for it, we have completed this invention.

[0009] In other words, in a preferred embodiment of the present invention, the following compositions or methods are provided. Section 1. Brown algae processing residue, liquor charcoal processing residue, alcoholic beverage processing residue, vegetable oil processing residue, palmitrein Acids and other unsaturated fatty acids or their salts, essential oil processing residues, citrus peels and their processing residues, and, It contains at least one selected from the group consisting of oil and its processing residue as an active ingredient. Oral composition for suppressing methane gas generation; Section 2. The brown algae processing residue is the residue after extracting fucoidan from brown algae, as described in item 1. Oral composition. Section 3. The oral composition according to item 1, wherein the sake charcoal processing residue is activated carbon residue after sake filtration processing. Section 4. The oral composition according to item 1, wherein the alcoholic beverage processing residue is lees derived from white grape wine. Section 5. The oral composition according to item 1, wherein the processed residue of the alcoholic beverage is lees derived from red wine. Item 6. The oral composition according to any one of items 1 to 5, wherein the processed residue of brown algae, processed residue of sake charcoal, or processed residue of alcoholic beverage has been subjected to heat treatment or drying treatment. The oral composition according to any one of items 1 to 5, wherein the processed residue of brown algae, processed residue of sake charcoal, or processed residue of alcoholic beverage has been subjected to heat treatment or drying treatment. Item 7. The oral composition according to any one of items 1 to 6, which is a feed or a raw material thereof. Item 8. Providing at least one selected from the group consisting of processed residue of brown algae, processed residue of sake charcoal, processed residue of alcoholic beverage, processed residue of vegetable oil, unsaturated fatty acids such as palmitoleic acid and salts thereof, processed residue of essential oil, citrus peel and processed residue thereof, and curd and processed residue thereof to ruminants, Providing at least one selected from the group consisting of processed residue of brown algae, processed residue of sake charcoal, processed residue of alcoholic beverage, processed residue of vegetable oil, unsaturated fatty acids such as palmitoleic acid and salts thereof, processed residue of essential oil, citrus peel and processed residue thereof, and curd and processed residue thereof to ruminants, Providing at least one selected from the group consisting of processed residue of brown algae, processed residue of sake charcoal, processed residue of alcoholic beverage, processed residue of vegetable oil, unsaturated fatty acids such as palmitoleic acid and salts thereof, processed residue of essential oil, citrus peel and processed residue thereof, and curd and processed residue thereof to ruminants, and a method for suppressing methane gas production by the ruminants.

Advantages of the Invention

[0010] According to the present invention, it is possible to provide a composition for suppressing the production of methane gas by ruminants. It is possible.

Brief Description of the Drawings

[0011] [Figure 1] Figure 1 is a graph showing the test results using the processed residue of mozuku in Test Example 1 of the examples. [Figure 2] Figure 2 is a graph showing the test results using the processed residue of white wine in Test Example 1 of the examples. [Figure 3] Figure 3 is a graph showing the test results using the processed residue of red wine in Test Example 1 of the examples. [Figure 4] Figure 4 is a graph showing the test results using sake charcoal in Test Example 1 of the examples. [Figure 5] Figure 5 is a graph showing the test results using the processed residue of macadamia nut oil in Test Example 2 of the examples. [Figure 6] Figure 6 is a graph showing the test results using palmitoleic acid in Test Example 2 of the examples. [Figure 7] Figure 7 is a graph showing the test results using the lemon myrtle processing residue from Test Example 2 of the Examples. [Figure 8] Figure 8 is a graph showing the test results using awamori lees in Test Example 2 of the Examples. [Figure 9] Figure 9 is a graph showing the test results using kale from Test Example 2 of the Examples. [Figure 10] Figure 10 is a graph showing the test results using charcoal in Test Example 3 of the Examples. [Figure 11] Figure 11 is a graph showing the test results using hassaku orange peel in Test Example 3 of the Examples. [Modes for carrying out the invention]

[0012] In this specification, the unit of content "mass%" is synonymous with "g / 100g".

[0013] In this specification, "salt" refers to a pharmaceutically acceptable salt. It is not limited to, for example, an organic base. Salts of (for example, trimethylamine salt, triethylamine salt, monoethanolamine salt, Salts with tertiary amines such as triethanolamine salts and pyridine salts, arginine, lysine, etc. Basic ammonium salts, etc.), salts with inorganic bases (e.g., ammonium salts, sodium salts, Alkali metal salts such as potassium salts, alkaline earth metal salts such as calcium salts and magnesium salts (Aluminum salts, etc.), salts with organic acids (citrates, lactates, phosphates, tartrates, gluten) (Conate, succinate, glutamate, aspartate, pyrrolidone carboxylate) Examples include salts with inorganic acids (hydrochloride, sulfate, nitrate, phosphate, borate, etc.).

[0014] In this specification, ruminants are animals that have multiple stomachs and bring food back from their stomachs to their mouths before digestion. The act of chewing and swallowing plants containing the cellulose is performed by symbiotic microorganisms, which then produce cellulose and other substances. This refers to animals that use the breakdown of high-molecular-weight compounds as an energy source. Examples include cows, goats, sheep, and laurels. This includes animals such as munchers, bison, deer, wildebeest, antelopes, camels, or llamas. Rumination In the rumen of an animal's stomach, symbiotic microorganisms cause plant cellulose to volatilize into methane. It is converted into sexually transmitted fatty acids.

[0015] [Oral composition for suppressing methane gas generation] The oral composition for suppressing methane gas production of the present invention is derived from brown algae processing residue, sake charcoal processing residue, and sake charcoal processing residue. Processing residues, vegetable oil processing residues, palmitoleic acid or its salts, essential oil processing residues, citrus peels and At least one selected from the group consisting of the processing residues of bison and kale and its processing residues. It contains one type as an active ingredient.

[0016] (Brown algae processing residue) A composition according to one aspect of the present invention contains brown algae processing residue as an active ingredient. Brown algae are For example, mozuku, fumarole, sea fan, kelp, makonbu, wakame, amijikusa, meka Bu (the root part of wakame), Arame, Kurome, Hijiki, Akamoku, Giant Kelp, Bu These include kelp, and the processing residue derived from one or more combinations of these is used. It is possible.

[0017] Processing residues in brown algae include, for example, those from which fucoidan, a component of seaweed, has been extracted. This refers to the residue that remains after extraction. Extraction is performed using, for example, an acidic aqueous solution such as an acetic acid aqueous solution or dilute hydrochloric acid with a pH of about 2. Any known method may be used, such as acid extraction or hot water extraction. However, it is not limited to this method. For example, in the acid extraction method, brown algae are dried, crushed, and an acidic aqueous solution is added to extract fucoidan The process involves solubilizing the substance, extracting it, neutralizing the solution, and then separating the liquid using methods such as centrifugation to remove the supernatant. The solid obtained can be used as processing residue. In the hot water extraction method, brown algae are used. After cutting or crushing to an appropriate size, immerse in hot water at approximately 90-100°C for a certain period of time (for example, 1 By immersing and heating for approximately 3 hours, soluble components including fucoidan are extracted. Yes. Afterwards, the extract is subjected to solid-liquid separation treatment by filtration or centrifugation, and the solid content after extraction is obtained. It can be obtained as a processing residue.

[0018] Preferably, the processing residue of mozuku is used. Mozuku is, for example, from the family Nagamatsumori (Chorthonaceae). (dariaceae), genus Cladosiphon okamuran Even if it is usTOKIDA, it belongs to the family Spermatochnaceae, the shrike It may also belong to the genus Nemacytis dicipieus Kuckuck. The residue from processing Okinawa mozuku, which contains a large amount of fucoidan in its polysaccharide components, is desirable. It can be used in various ways.

[0019] The acid concentration of the above acidic aqueous solution is usually at least 0.05 M, preferably around 0.07-2 M. The ratio of brown algae to water is, for example, 10-500 mL of water per 1 g of dry material, preferably... Alternatively, add approximately 30-200 mL.

[0020] Whether using commercially available brown algae processing residue or using residue obtained by known methods... It may be used.

[0021] Brown algae processing residues typically contain fucose, galactose, glucose, xylose, It also contains polysaccharides including uronic acid and cellulose. The molecular weight of these polysaccharides is usually 4 It can range from 0,000 to 1,000,000. The residue after extracting the active ingredient is the initial mass. It is approximately 0.5 to 20% by mass.

[0022] In the composition of the present invention, the content of brown algae processing residue relative to the total amount of the composition is preferably is 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass or more This may be 4% by mass or more, 5% by mass or more, etc. Brown algae processing of the total amount of the composition of the present invention The residue content is preferably 20% by mass or less, 15% by mass or less, 10% by mass or less, and 5% by mass. The amount may be less than 1%.

[0023] (Sake charcoal processing residue) Another embodiment of the present invention contains a composition containing sake charcoal processing residue as an active ingredient. The charcoal processing residue used in this process is a filtration process for brewed alcoholic beverages such as sake and distilled alcoholic beverages such as whiskey. This refers to activated charcoal that has been used for a certain period of time. Examples of brewed beverages include sake, beer, mirin, and plums. Examples include alcohol and wine. Distilled spirits are made by distilling fermented alcohol, which is evaporated once and then condensed again. This refers to alcoholic beverages produced through a specific process, including brandy, shochu, and whiskey.

[0024] Activated carbon treatment is performed to remove unwanted components present in brewed alcoholic beverages. Here, unwanted components of alcohol refer to pigments, proteins, lipids, ash, etc. The specific processing method and For example, a known method involves adding activated carbon to the liquid produced during the brewing process of alcoholic beverages, stirring, and letting it stand. This could be the method.

[0025] Activated carbon is not limited to this, but is typically used in the production of alcoholic beverages. It is made from materials such as cypress, cedar, maple, beech, bamboo, oak, sawtooth oak, evergreen oak, and Japanese evergreen oak. It can be prepared by [method].

[0026] Sake charcoal processing residue is the activated carbon residue remaining after removing unwanted components contained in such brewed alcoholic beverages. This refers to the fact that whether you use commercially available products or products obtained by known methods... good.

[0027] There are no limits on the amount of activated carbon used in the process, but for example, in the manufacturing process of brewed alcoholic beverages... For every 1 kL of liquid produced, 0.1 to 2 kg of activated carbon can be used.

[0028] The activated carbon after processing has adsorbed substances generated during the sake manufacturing process. These substances include: Examples include proteins, fats, fibers, ash, and soluble non-nitrogenous substances.

[0029] In the composition of the present invention, the content of sake charcoal processing residue relative to the total amount of the composition is preferably , 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass or more, This may be 4% by mass or more, 5% by mass or more, etc. Brown algae processing residue relative to the total amount of the composition of the present invention The residue content is preferably 20% by mass or less, 15% by mass or less, 10% by mass or less, and 5% by mass It can be less than %.

[0030] (Alcoholic beverage processing residue) Another embodiment of the present invention contains alcohol processing residue as an active ingredient. Industrial residue is a by-product (lees) produced during the manufacturing process of alcoholic beverages, and is also known as yeast fermentation material. This is the case. The lees of such alcoholic beverages include sake, shochu (awamori, etc.), fruit wines (grape wine, etc.), and This includes processing residues from whiskey, spirits, liqueurs, beer, and sparkling alcoholic beverages. In particular, wine processing residues and awamori processing residues are preferably used.

[0031] <Wine processing residue> Wine is a fermented alcoholic beverage made from grapes, and includes still wine and sparkling wine. This includes all types of Kling wine. While there are no specific restrictions on the type of grape used as the raw material, white grapes are preferred. Examples of grape varieties used as raw materials for wine include Chardonnay, Sauvignon Blanc, and Sémillon. The raw materials for red wine include Cabernet Sauvignon, Merlot, and Pinot Noir. Examples are given.

[0032] There are no particular limitations on the method for obtaining winemaking residue; conventional methods can be used. In other words, the grapes are crushed to extract the juice, which is then fermented. In the case of white wine... Since only the juice is extracted and fermented, solid materials such as the peel, seeds, and stems are removed before fermentation. In the case of red grape wine, the juice, skins, and seeds are fermented together. After fermentation, the solids are pressed. After fermentation is complete, the wine is pressed. What remains at this point are the grape skins, seeds, and some of the pulp. It becomes a winemaking residue containing [unspecified substances].

[0033] Here, either red or white wine can be used, but the processing residue of white wine is particularly preferred. It can be used. The processing residue of white wine contains grape skins, pectin, dietary fiber, seeds, It contains the remaining stems and pulp, but the anthocyanin and phenol content is similar to that of red grape wine processing residues. This value is lower than that of slag. Materials containing residual terpenes can also be used.

[0034] <Awamori processing residue> Awamori processing residue is the residue generated during the production process of awamori. Awamori is typically made from Thai rice ( After adding black koji to indica rice to make rice koji, and then adding water and yeast to prepare the mash, By fermenting alcohol, a matured mash is produced, and by distilling this matured mash... It is manufactured. The distillation residue from the distillation process of the aged mash is the awamori processing residue. There are no particular restrictions on the dregs used in the production of awamori, such as Thai rice, black koji, and yeast.

[0035] The components of the awamori processing residue are not particularly limited, but typically include, for example, moisture (95% ± 3%). %), Crude protein (3%±1%), Crude fiber (1%±1%), Crude fat (0.5%±0.5%) This is expressed as %, crude ash (0.1% ± 0.1%), etc. Furthermore, because the processing residue of awamori contains organic acids, its pH is low and it is weakly acidic. Specifically The range is approximately 3.0 to 6.0.

[0036] Even if commercially available alcohol processing residues are used, only those obtained by known methods should be used. You may do so.

[0037] In the composition of the present invention, the content of alcoholic beverage processing residue relative to the total amount of the composition is preferably , 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass or more, This may be 4% by mass or more, 5% by mass or more, etc. Brown algae processing residue relative to the total amount of the composition of the present invention The residue content is preferably 20% by mass or less, 15% by mass or less, 10% by mass or less, and 5% by mass It can be less than %.

[0038] (Vegetable oil processing residue) A composition according to another aspect of the present invention contains a vegetable oil or its extract residue as an active ingredient. It contains. Vegetable oils are liquid or semi-solid substances obtained from plant-derived seeds, fruits, kernels, etc. It is a type of oil and fat widely used in the food, cosmetics, and pharmaceutical industries. Examples of oils include macadamia nut oil, olive oil, jojoba oil, almond oil, and shea oil. Examples include fats, sunflower oil, etc., all of which can be obtained by known methods.

[0039] In particular, macadamia nut oil is produced in Australia, South Africa, Kenya, and other countries where macadamia nuts are cultivated. It is a liquid vegetable oil obtained from macadamia nut seeds by methods such as cold pressing and solvent extraction. The main components are palmitoleic acid, myristic acid, palmitic acid, hexadecenoic acid, and It contains fatty acids such as thearic acid, oleic acid, linoleic acid, and arachidic acid.

[0040] Extraction residues of vegetable oils refer to the residues obtained after extracting vegetable oils, such as pressed cake and defatted cake. This refers to the solid content, which includes dietary fiber, protein, trace lipids, phenolic compounds, minerals, etc. Sometimes it happens.

[0041] Vegetable oils or their extraction residues may be commercially available or obtained by known methods. You may use the ones that have been prepared.

[0042] In the composition of the present invention, the content of vegetable oil processing residue relative to the total amount of the composition is preferably Or, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass The above amounts may be 4% by mass or more, 5% by mass or more, etc. Brown algae relative to the total amount of the composition of the present invention The processing residue content is preferably 20% by mass or less, 15% by mass or less, or 10% by mass or less. It may be less than 5% by mass.

[0043] (Palmitoleic acid or its salts) A composition according to another aspect of the present invention comprises palmitoleic acid or a salt thereof as an active ingredient. To possess.

[0044] In the composition of the present invention, the content of palmitoleic acid or its salt relative to the total amount of the composition Preferably, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, It may be 3% by mass or more, 4% by mass or more, 5% by mass or more, etc. (relative to the total amount of the composition of the present invention) The content of brown algae processing residue is preferably 20% by mass or less, 15% by mass or less, and 10% by mass It may be less than % or less than 5% by mass.

[0045] (Essential oil processing residue) Another embodiment of the present invention is a composition comprising essential oil plants or their extracts, and their essential oil extracts. The processing residue obtained after production is included as an active ingredient. Essential oil plants are aromatic volatile components. This refers to plants that contain essential oils, with leaves, flowers, fruit peels, bark, wood, roots, etc., being used as extraction materials. It is possible.

[0046] Examples of plants that produce essential oils include lemon myrtle, tea tree, eucalyptus, cajeput, and lavender. Dar, rosemary, yuzu, mint, kuromoji, ginger lily, lemongrass, shiso, ka Boss, Sudachi, Sandalwood, Blue Cypress, Orange, Lavender, Peppermint Examples include lemongrass and clary sage.

[0047] For example, lemon myrtle is an evergreen plant belonging to the Myrtaceae family. It is a plant, mainly native to northern Australia, but there are also examples of cultivation in Japan. (Lemon Mart) The leaves of the plant contain abundant essential oil components and can be used dried or in their moist state. The extract is It is mainly obtained from the leaves by methods such as stirring extraction under heating, reflux extraction, steam distillation, and solvent extraction. It is possible.

[0048] The solvent used for the extraction is not particularly limited, but may include water (room temperature water, warm water, hot water), etc. Examples of polar or neutral solvents include nitrates, propylene glycol, glycerin, and vegetable oils. The processing residue remaining after essential oil extraction, which includes leaf parts, fibers, cell wall components, and water-soluble components, is... It retains some of the essential oil components and may contain functional components found in essential oils.

[0049] The composition of the present invention contains lemon myrtle or its extract in the total amount of the composition. The amount is preferably 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, or 2% by mass or more. It may be 3% by mass or more, 4% by mass or more, 5% by mass or more, etc. (This is relative to the total amount of the composition of the present invention.) The content of brown algae processing residue is preferably 20% by mass or less, 15% by mass or less, and 10% by mass. It may be less than a certain percentage, or less than 5% by mass.

[0050] (Citrus peels and their processing residues) A composition according to another aspect of the present invention is a citrus peel, a citrus peel extract, or an additive thereof. It contains the industrial residue as an active ingredient. Citrus fruits here refer to, for example, mandarin oranges (Unshu oranges) Mandarin orange, hassaku orange, yuzu orange, bitter orange, kabosu orange, sudachi orange, lemon, shikwasa orange, orange In addition to varieties cultivated in Japan such as oranges and grapefruit, varieties from Australia and New Zealand are also available. Finger limes, bush limes, and Australian desert limes found in Zealand Lime, navel orange, Valencia orange, mandarin, tangelo, lime, caramel This includes citrus fruits such as munchies and blood oranges. The varieties, origins, and cultivation methods used are not particularly special. Not limited to this.

[0051] The peel can be used raw, but it should be dried, crushed, roasted, steamed, fermented, or used for essential oil extraction. Processed products may be used. The part to be used may be either the exocarp or the endocarp, It may include both.

[0052] The processing residue of citrus peel used in this invention refers to the active ingredients (essential oils, extracts, and petroleum jelly) derived from the fruit peel. This refers to the solid residue obtained after separating and extracting substances such as kuchin.

[0053] Extraction methods from citrus peels include water, ethanol, aqueous ethanol, glycerin, Room temperature extraction using distilled water, heated extraction, reflux extraction, pressing, ultrasonic extraction, distillation extraction (essential oil extraction) The following can be used: [list of materials], and the residue after extraction can be used as an active ingredient in the composition as appropriate.

[0054] In the composition of the present invention, the content of citrus peel and its processing residue relative to the total amount of the composition is Preferably, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3 This may be % by mass or more, 4% by mass or more, 5% by mass or more, etc., relative to the total amount of the composition of the present invention. The content of brown algae processing residue is preferably 20% by mass or less, 15% by mass or less, or 10% by mass. The following may be 5% by mass or less.

[0055] (Kale or its processing residue) A composition according to another aspect of the present invention comprises kale, kale extract, or processing residue thereof. It contains active ingredients. Kale is a plant belonging to the Brassicaceae family. The variety of kale is not particularly restricted; kitchen kale, tree kale, bush kale, etc. Use various types of kale such as marrow kale, coriander, and green leaf kale. It is possible. Kale can be used raw, but it can also be dried, crushed, roasted, freeze-dried, or steamed. It may be processed by boiling, fermentation, or other methods. Leaves are preferred, and stems are also included. That's good too.

[0056] Kale extracts include those obtained by squeezing kale or extracting it with a solvent. Liquid, pe It can be in any form, such as solid or extract powder. The extract is obtained from kale using a solvent. It is possible.

[0057] The solvent is not limited to water (room temperature water, warm water, hot water), ethanol, or hydrated ethanol. Examples include Knoll, etc.

[0058] In the composition of the present invention, the content of kale or its processing residue relative to the total amount of the composition is: Preferably, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% The amount may be % by volume or more, 4% by mass or more, 5% by mass or more, etc. Brown relative to the total amount of the composition of the present invention. The content of algae processing residue is preferably 20% by mass or less, 15% by mass or less, or 10% by mass or less. It may be less than 5% by mass.

[0059] (Other ingredients) In addition to the above components, the composition of the present invention further contains various components such as molasses, salt, vitamins, and enzymes. One or more types of these can be used in combination.

[0060] (Additives) The composition of the present invention does not impair quality such as appearance stability or viscosity, and does not impair the effects of the present invention. Within the quantitative and qualitative limits, various commonly used components such as excipients may be added as needed. It can be used.

[0061] (feed) The compositions of the present invention are typically used when mixed with solid feed and fed simultaneously with the solid feed. It is possible to do so. Alternatively, it can be given to animals separately from solid feed. It can also be prepared as a raw material, together with regular solid feed. The ingredients of the feed are not limited to those commonly used. Solid feed is made from grass. It is a solid feed made by grinding, mixing, and drying grains and other materials. Examples of feed ingredients include Examples include grains and nuts, oilseed meal, rice bran, manufacturing residues, animal-derived components, etc. These can be mixed and prepared.

[0062] Even if commercially available solid feed is used, it must be manufactured using a known manufacturing method. You may use it.

[0063] The form of solid feed is not particularly limited, but for example, it can be in the form of powder, pellets, or flakes. These include: The water content of solid feed should be 15% by mass or less, more specifically, 5% by mass to 1% It is preferable to adjust it to 5% by mass.

[0064] The feed ingredients include brown algae processing residue, sake charcoal processing residue, alcohol processing residue, vegetable oil processing residue, and refined A group consisting of oil processing residues, citrus peels and their processing residues, and kale and its processing residues. The ratio of at least one selected type to be mixed is not particularly limited, but solid feed 1 0.1 parts by mass to 90 parts by mass, 1 part to 80 parts by mass, 10 parts by mass to 00 parts by mass 80 parts by mass, 10 parts by mass to 90 parts by mass, 20 parts by mass to 70 parts by mass, or 30 parts by mass to 40 parts by mass This can be a mass component, etc.

[0065] The feed ingredients include at least one selected from the group consisting of palmitoleic acid or its salts. The mixing ratio is not particularly limited, but for example, per 100 parts by mass of solid feed, 0.01 parts by mass to 5 parts by mass, 0.05 to 4 parts by mass, 0.1 to 3 parts by mass, 0.5 to 2 parts by mass This can be done as follows.

[0066] (Manufacturing method) The method for producing the composition of the present invention is not particularly limited, and the stirring method, the order in which the components are added, the speed at which they are added, etc. This can be set as appropriate. Here, the raw materials are brown algae processing residue, sake charcoal processing residue, sake processing residue, and plants. Residues from the processing of natural oils and fats, palmitoleic acid or its salts, residues from the processing of essential oils, citrus peels and their processing residues At least one selected from the group consisting of slag and kale and its processing residue is composed Heat treatment or drying treatment can also be applied before the preparation of the material. Heat treatment may involve heating at approximately 60-120°C for 10 minutes to 2 hours. Heating methods include direct flame heating, steam heating, hot air heating, oven heating, and microwave heating. It is usable. Drying processes include, for example, hot air drying at around 40-80°C, vacuum drying, freeze drying, and spray drying. (i) Methods such as drum drying are used. Drying conditions depend on the properties of the raw material and the desired moisture content. The appropriate selection is made depending on the quantity.

[0067] (form) The composition of the present invention is not particularly limited as long as it is in a form that is easily mixed with feed, and can be used as is. It can be provided in a dry state.

[0068] (Application) The composition of the present invention is intended to suppress methane gas production in ruminants, and can be used as a feed ingredient or in animal feed. It is provided to ruminants along with other foods. The proportion of provision can be the same as the frequency of normal feeding. It can also be done once a day or once every two days.

[0069] [Methods to suppress methane gas generation] This invention also relates to brown algae processing residues, sake charcoal processing residues, alcohol processing residues, vegetable oil processing residues, Palmitoleic acid or its salts, essential oil processing residues, citrus peels and their processing residues, and ka To provide ruminants with at least one substance selected from the group consisting of ru and its processing residues. This also relates to a method for suppressing methane gas production by ruminants. The content, ratio, and other conditions of each component, as well as the manufacturing method, in [methane gas generation suppression]. The contents described in [Oral Composition for Control] shall be as specified. [Examples]

[0070] Next, the present invention will be specifically described with reference to examples, but the present invention is limited to the following examples. It is not that. Note that the units for each component amount in the table are in mass percent.

[0071] The test substances used in the following evaluations are as follows: Mozuku processing residue: Residue after fucoidan extraction (manufactured by Hokugan Co., Ltd.), total polyphenol amount , 41.31mg / gDM (dry weight) White wine processing residue: Chardonnay grape, total polyphenol content: 25.41 mg / gDM (dry (Quantity) Red wine processing residue: Merlot grape, total polyphenol content: 41.12 mg / gDM (dry weight) amount) Sake charcoal processing residue: Activated carbon residue used for filtration after sake brewing (commercially available activated carbon, wood raw material steamed) Micropores formed by the air activation method; specific surface area: approximately 900-1600 m² 2 / g, total pores Volume: approx. 0.4-1.6 ml / g, average pore diameter: approx. 1.4-3.6 nm), total polyphenols The amount of ol is 12.2 mg / gDM (dry matter). Macadamia nut oil: Made from raw materials sourced from Australia or Spain, manufactured by Nisshin Oillio Co., Ltd. Palmitoleic acid: Manufactured by Sigma-Aldrich Japan Co., Ltd. Lemon Myrtle Essential Oil: Made from Australian lemon myrtle leaves, manufactured by Krutti Co., Ltd. Awamori lees: Distillation residue of rice (mainly Thai rice) fermented using black koji mold (Clay Okinawa Co., Ltd.) (Manufactured) Kale processing residue: Sulforaphane-rich kale produced through variety improvement (Grand Green Co., Ltd.) (Manufactured by the company)

[0072] [Test Example 1: Evaluation of Methane Gas Production by Lumen Fluid Culture 1] 40 ml of rumen contents collected from two Saanen hybrid goats, mixed with basic feed (commercially formulated feed). Nutritional information (per 0.6 g dry matter): Crude protein approx. 16%, crude fat approx. 2%, crude fiber approx. 10%, crude ash (approximately 10%), Sudan grass 0.4g (dry matter: crude protein approximately 5%, crude fat approximately 2%) A control group was supplemented with only crude fiber (approximately 32%) and crude ash (approximately 13%

[18] ) and 10% of the basal feed was added. Tests were conducted by adding various materials (mozuku processing residue, white wine lees, red wine lees, and sake charcoal) to each. A group was established, and culture solutions for each group were prepared in 200 ml Erlenmeyer flasks. After preparing the culture solutions, After replacing the nutrient solution with nitrogen gas, the cells were cultured anaerobically at 39°C for 3 hours. The experiment was repeated three times. The methane gas concentration was measured using gas chromatography, with a portion of the gas sampled at each incubation time. Measurements were taken using (GC390B, GL Science). A packed column (Shi) was used for the analysis column. ncarbon ST) is used, and a thermal conductivity detector (TCD) is used as the detector, with the inlet and detection The output temperature was set to 100°C. Nitrogen was used as the carrier gas. Statistical analysis was performed using St. A t-test was used for udents.

[0073] The results for mozuku processing residue, white wine processing residue, red wine processing residue, and sake charcoal are shown in Figures 1-4. These are shown. The results are the average of three trials. Error bars represent the mean ± standard error (SEM). The results are shown. * indicates a statistically significant difference compared to the control group (p<0.05, n=3) (the same applies below).

[0074] As shown in Figures 1-2, the methane gas concentration is found in the mozuku processing residue and the white wine processing residue (white wine In the case of fermented rice lees, the effect was significantly suppressed compared to the control. Mozuku seaweed processing residue and white wine processing residue (White wine lees) did not affect the number of protozoa, therefore, processing of mozuku seaweed into ruminant livestock is not recommended. The addition of residues and white wine processing residues (white wine lees) to feed does not affect feed digestibility. The results suggest the possibility of significantly reducing methane gas emissions.

[0075] As shown in Figures 3-4, the methane gas concentration is as follows: red wine processing residue (red wine lees) and sake charcoal processing In the case of processing residues (sake charcoal), the effect was suppressed compared to the control. Red wine processing residues (red wine lees) and Since the residue from sake charcoal processing (sake charcoal) did not affect the number of protozoa, it was determined that red blood cells in ruminant livestock did not affect protozoa. The addition of wine processing residue (red wine lees) and sake charcoal processing residue (sake charcoal) to feed affects feed digestibility. The possibility of suppressing methane gas emissions without causing any impact was suggested. The lees (Chardonnay grape) have a higher total polyphenol content compared to the red wine processing residue (Merlot grape). Despite the lower dose (25.41 mg / DMg vs. 41.12 mg / DMg), It showed a significant methane gas suppression effect. This result indicates that simple methane gas suppression is effective. It's not just the total amount of polyphenols that contributes to the effect, but also other components besides polyphenols, This suggests that the fibrous structure of the residue and its physical properties related to digestibility may be interacting in a complex way. They are doing it.

[0076] [Test Example 2: Evaluation of Methane Gas Production by Lumen Fluid Culture 2] 40 ml of rumen contents collected from two Japanese Black cattle were used as a base feed, similar to the method used in Test Example 1. A control group to which only was added, and a group to which 10% of various materials (for example, vegetable oil processing residues) were added to the base feed. (The ingredients are macadamia nut oil, palmitoleic acid, lemon myrtle, awamori lees, and kale) A test plot was prepared with the additive added, and after purging with nitrogen gas as in Test Example 1, it was cultured anaerobically at 39°C for 3 hours. A cultivation test was conducted. Methane gas concentration was measured using gas chromatography by taking a sample of the gas at each cultivation time. Measurements were taken using matrixing (GC390B, GL Science). Packed ionography was performed on the analytical column. A column (Shincarbon ST) and a thermal conductivity detector (TCD) were used. The temperature of the inlet and detector was set to 100°C. Nitrogen was used as the carrier gas. The analysis was performed using Student's t-test. Statistical analysis was performed using Student's t-test.

[0077] Vegetable oil processing residue (macadamia nut oil), palmitoleic acid, essential oil processing residue (lemon The results for nummart, alcoholic beverage processing residue (awamori lees), and kale are shown in Figures 5-9, respectively. Results This is the average of three trials.

[0078] As shown in Figures 5-9, vegetable oil processing residues (macadamia nut oil) are used for ruminant livestock. Mitoleic acid, essential oil processing residue (lemon myrtle), alcohol processing residue (awamori lees), kale additive The supplementation was suggested to have the potential to reduce methane gas emissions. It is difficult to completely remove oil during the oil extraction process, and some oily components are present. For example, macadamia Nut residue contains macadamia nut oil and palmitoleic acid, a constituent fatty acid. As mentioned above, macadamia nut oil and palmitoleic acid have an effect of suppressing methane gas production. Since methane gas was detected, it was also found in macadamia nut residue, which is a processing residue for vegetable oils. It is thought that a growth-inhibiting effect is achieved. Similarly, as essential oil processing residue, essential oil processing Complete removal of oil is difficult, and it contains some essential oil components. For example, lemon myrtle essential oil Lemon myrtle essential oil components remain in the oil processing residue. As mentioned above, lemon myrtle Since the effect of suppressing methane gas production was confirmed in the lemon myrtle essential oil processing residue, the same effect was observed in the lemon myrtle essential oil processing residue. This is thought to have an effect of suppressing methane gas production.

[0079] [Test Example 3: Evaluation of Methane Gas Production by Lumen Fluid Culture 3] Rumen fluid was collected from two Japanese Shorthorn breeding cows, which are primarily fed roughage, before their morning feed. Oral samples were used. The collected rumen fluid was filtered through triple gauze, and the filtrate was 100%. A control group was given only basal feed (2.0g of pasture grass) in ml, and basal feed was given hassaku peel, Two groups were prepared: one with 0.20g of sake charcoal added to the hassaku orange, and the other with sake charcoal added to the sake charcoal. Culture solutions for each group were prepared in Erlenmeyer flasks. After preparing the culture solutions, nitrogen gas was passed through the culture solutions, and 3 The samples were cultured anaerobically at 39°C for several hours. The experiment was repeated three times. Gas production was measured during each culture cycle. Using the gas collected in between, a gas chromatograph (GC-3200, GL Science) is used. Measurements were taken using s). Statistical analysis was performed using Student's t-test.

[0080] The results for sake charcoal (dried at 40°C) and citrus peel processing residue are shown in Figures 10 and 11, respectively. Results This is the average of three trials.

[0081] As shown in Figures 10-11, alcohol charcoal (dried at 40°C) and citrus peel processing residues are added to ruminant livestock. The supplemental feeding was suggested to potentially reduce methane gas emissions.

Claims

1. It contains at least one selected from the group consisting of brown algae-derived fucoidan extract residue, macadamia nut oil extract residue, lemon myrtle essential oil processing residue, charcoal processing residue, and kale and its processing residue. The charcoal processing residue is the activated carbon residue after filtration of sake. Oral composition for suppressing methane gas generation.

2. The oral composition according to claim 1, wherein the fucoidan extraction residue derived from brown algae, or the charcoal processing residue, has been subjected to heat treatment or drying treatment.

3. An oral composition according to claim 1, which is a feed or a raw material thereof.

4. This includes providing ruminants with at least one selected from the group consisting of brown algae-derived fucoidan extract residue, macadamia nut oil extract residue, lemon myrtle essential oil processing residue, charcoal processing residue, and kale and its processing residue. The charcoal processing residue is the activated carbon residue after filtration of sake. A method for suppressing methane gas production by ruminants.

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