Liquid chromatograph system and control method

Abstract

In a liquid chromatograph system (10), a plurality of streams (60A, 60B, 60C, 60D) separate a sample. A sample injection device (18) injects a sample into each of the plurality of streams (60A, 60B, 60C, 60D). A detector (500) is disposed downstream of the plurality of streams (60A, 60B, 60C, 60D) and analyzes samples that have been separated by the plurality of streams (60A, 60B, 60C, 60D). A control device (110) stores, for each sample, an analysis condition for sample separation. The plurality of streams (60A, 60B, 60C, 60D) include at least two streams that have mutually different stream configurations related to sample separation. The control device (110) selects, from among the plurality of streams, a stream that has a stream configuration corresponding to the analysis condition for a sample.

Description

Liquid chromatograph system and control method

[0001] The present disclosure relates to liquid chromatography systems and control methods, and more particularly to techniques for allocating analysis in a liquid chromatography system having multiple streams.

[0002] Liquid chromatography is a technique for separating components contained in a sample by introducing the sample to be analyzed into a column together with a mobile phase (eluent). The components of the sample separated by liquid chromatography are then analyzed using a detector such as a mass spectrometer.

[0003] U.S. Patent Application Publication No. 2022 / 0137011 (Patent Document 1) and "Nexera QX" by Shimadzu Corporation (Non-Patent Document 1) describe analytical systems having multiple streams each containing multiple columns. The analytical systems described in Patent Document 1 and Non-Patent Document 1 connect any of the multiple streams to a mass spectrometer via a valve having multiple ports connected to the mass spectrometer.

[0004] US Patent Application Publication No. 2022 / 0137011

[0005] "Nexera QX", [online], [searched December 14, 2022], Shimadzu Corporation, Internet <URL: https: / / www.ssi.shimadzu.com / products / liquid-chromatography-mass-spectrometry / ultrafast-multiplex-lcms-nexera-qx.html>

[0006] According to the analytical systems described in Patent Document 1 and Non-Patent Document 1, analytical efficiency can be improved by continuing mass spectrometry using multiple streams. However, even in analytical systems including multiple streams, the configurations of each stream (e.g., type of mobile phase, type of column) may differ, and samples with different analytical conditions cannot be analyzed depending on the stream.

[0007] An object of the present disclosure is to efficiently analyze samples with different analytical conditions using a liquid chromatographic system including multiple streams.

[0008] A liquid chromatography system according to one aspect of the present disclosure is a liquid chromatography system for analyzing multiple samples. The liquid chromatography system includes streams, a sample injection device, a detector, and a control device. The multiple streams separate samples. The sample injection device injects a sample into each of the multiple streams. The detector is disposed downstream of the multiple streams and analyzes the sample separated in each of the multiple streams. The control device stores analysis conditions for separating the sample for each sample. Each of the multiple streams includes a column and a supply device. The column separates the sample injected into the stream. The supply device supplies a mobile phase used in the column. The multiple streams include at least two streams having different stream configurations for sample separation. The control device selects a stream from the multiple streams having a stream configuration corresponding to the analysis conditions for the sample.

[0009] A control method for a liquid chromatograph system according to another aspect of the present disclosure is a computer-implemented control method for a liquid chromatograph system for analyzing multiple samples. The liquid chromatograph system includes streams, a sample injection device, and a detector. The multiple streams separate samples. The sample injection device injects a sample into each of the multiple streams. The detector is disposed downstream of the multiple streams and analyzes the sample separated in each of the multiple streams. Each of the multiple streams includes a column and a supply device. The column separates the sample injected into the stream. The supply device supplies a mobile phase for use in the column. The multiple streams include at least two streams having different stream configurations for sample separation. The control method includes the steps of selecting, from the multiple streams, a stream having a stream configuration corresponding to analytical conditions for sample separation, and performing sample analysis in the selected stream.

[0010] According to the present disclosure, samples with different analytical conditions can be efficiently analyzed using a liquid chromatographic system including multiple streams.

[0011] FIG. 1 is a schematic diagram of a liquid chromatograph system. FIG. 2 is a diagram for explaining a sample plate. FIG. 3 is a schematic diagram of a stream. FIG. 4 is a diagram for explaining the configuration of a control device. FIG. 5 is a flowchart showing processing related to control of a liquid chromatograph system. FIG. 6 is a flowchart for explaining processing for allocating stream-based analysis. FIG. 7 is a flowchart for explaining processing for allocating batch-based analysis. FIG. 8 is a flowchart for explaining processing for allocating plate-based analysis. FIG. 9 is a flowchart for explaining processing for performing priority analysis. FIG. 10 is an example of a display screen showing the progress of all analyses.

[0012] Hereinafter, embodiments of the present disclosure will be described in detail with reference to the drawings. In the drawings, the same or corresponding parts are designated by the same reference numerals, and description thereof will not be repeated.

[0013] 1 is a schematic diagram of a liquid chromatography system (hereinafter referred to as an LC (Liquid Chromatography) system) 10. The LC system 10 includes an analyzer 100 and a controller 110.

[0014] The analytical device 100 separates and analyzes components contained in a sample and includes a sample injection device 18, a plurality of analytical flow paths (hereinafter referred to as "streams") 60A to 60D, a divert valve 90, and a detector 500.

[0015] The sample injection device 18 is a device for injecting samples into each of the streams 60A to 60D. The sample injection device 18 is, for example, an autosampler, and includes one or more sample plates 80 (see FIG. 2) capable of accommodating one or more samples, and a needle (not shown). One sample injection device 18 typically includes several or more sample plates 80. Each sample plate 80 is managed, for example, by a plate number. One sample plate 80 accommodates, for example, 10 to several hundred samples. The sample plate 80 may be configured to accommodate samples housed in a container such as a vial, or may be configured to accommodate samples directly without a container, as in a microplate. Each sample plate 80 is typically assigned a sample number corresponding to the position where the sample is accommodated. For example, the sample accommodated at the position indicated by 81 in FIG. 2 is distinguished from other samples as sample number 1 on plate number 1. The needle aspirates a predetermined sample instructed by the control device 110 from the sample plate 80 and injects the aspirated sample into a predetermined stream instructed by the control device 110 .

[0016] The streams 60A to 60D are flow paths provided for separating the samples, and the mobile phase flows through them. Each of the streams 60A to 60D is connected to a detector 500 via a diverter valve 90.

[0017] The diverter valve 90 includes ports 91 to 96. The streams 60A to 60D are connected to ports 91 to 94, respectively. The detector 500 is connected to port 95. A drain pipe (not shown) is connected to port 96. The diverter valve 90 fluidly connects one of the streams 60A to 60D to the detector 500 by switching the connection destinations of the ports 91 to 94 between the port 95 and the port 96, respectively.

[0018] Detector 500 is disposed downstream of streams 60A-60D and is a device for analyzing the samples separated in each of streams 60A-60D. In one embodiment, detector 500 is a mass spectrometer that performs mass spectrometry on the samples. Note that detector 500 is not limited to a mass spectrometer, and may be an absorbance detector, a fluorescence detector, a differential refractive index detector, an electrical conductivity detector, an evaporative light scattering detector, or the like, and is not particularly limited thereto.

[0019] As described above, in the LC system 10, multiple streams 60A to 60D are connected in parallel and connected to the detector 500 via the diverter valve 90, so that sample separation can be performed in parallel using each of the streams 60A to 60D.

[0020] 2. Stream Configuration Next, the configuration of stream 60A will be described with reference to Fig. 3. Stream 60A includes a column 70A, a supply device 50A, and a valve 180A. The mobile phase is sent from supply device 50A toward column 70A, passes through column 70A, and is sent to a divert valve 90.

[0021] The supply device 50A is a device for supplying the mobile phase used in the column 70A. In the example of Fig. 3, the supply device 50A supplies the mobile phase flowing in stream 60A. The supply device 50A includes a plurality of mobile phase containers 20A, a mixer 30A, and a mobile phase pump 40A.

[0022] The multiple mobile phase containers 20A are containers that each contain a predetermined type of mobile phase. The mobile phase contained in the mobile phase container 20A is used as an eluent for sample separation in the column 70A. In one embodiment, the types of mobile phase contained in each of the multiple mobile phase containers 20A are different from each other. However, at least two of the mobile phases contained in each of the multiple mobile phase containers 20A may be the same. The types of mobile phase include, but are not limited to, types of mobile phases commonly used by those skilled in the art, such as water, acetonitrile, methanol, organic acids of a predetermined concentration, and acetone. Note that the types of mobile phase also include a mixture of at least two of water, acetonitrile, methanol, organic acids of a predetermined concentration, acetone, etc., premixed in a predetermined ratio. The mobile phase container corresponds to one example of a "container."

[0023] The mixer 30A mixes the mobile phases in the multiple mobile phase containers 20A at a set mixing ratio. The mixing ratio may be 0:100 or more. The mixing ratio is set, for example, by the control device 110 based on the analysis conditions of the samples to be separated. The mixing ratio may be set to be constant during the analysis of the samples, or may be set to change over time during the analysis.

[0024] The mixer 30A may mix the mobile phases contained in all of the mobile phase containers 20A contained in the stream 60A, or may mix only the mobile phases contained in at least two of the mobile phase containers 20A. Alternatively, the mixer 30A may use only the mobile phase contained in one of the mobile phase containers 20A. In these cases, the unmixed mobile phases have a mixing ratio of 0.

[0025] As will be described later, in the LC system 10, each supply device may be provided with one mobile phase container, in which case there is no need to install a mixer.

[0026] The mobile phase pump 40A is a pump for supplying the mobile phase to the column 70A at a predetermined pressure. In the example of FIG. 3 , the mobile phase pump 40A sends the mixed liquid mixed by the mixer 30A as the mobile phase toward the valve 180A. The mobile phase pump 40A corresponds to one example of a "pump" in this specification. Note that, although one mobile phase pump is connected downstream of the mixer 30A in the example of FIG. 5 , one mobile phase pump may be provided between each of the multiple mobile phase containers 20A and the mixer 30A.

[0027] The valve 180A switches the flow path connected to the supply device 50A between a flow path that passes through the sample injection device 18 and is connected to the column 70A, and a flow path that is connected to the column 70A without passing through the sample injection device 18. If the flow path in which the supply device 50A, the sample injection device 18, and the column 70A are connected in series in this order is called the "injection flow path," and the flow path in which the supply device 50A and the column 70A are connected in series in this order without passing through the sample injection device 18 is called the "direct flow path," then it can be said that the valve 180A switches between the injection flow path and the direct flow path.

[0028] Column 70A separates the sample injected into stream 60A. Column 70A is packed with a stationary phase for separating the components of the sample.

[0029] The configuration of stream 60A has been described in detail above. Streams 60B to 60D have the same configuration as stream 60A in that they include a flow path directly connected to the injection path, and also include a mobile phase container, a mobile phase pump, a high-pressure valve, and a column. However, at least two of streams 60A to 60D may have different stream configurations with respect to sample separation.

[0030] The stream configuration may include, for example, the type of mobile phase. In other words, the types of mobile phases contained in each stream may be different from each other. A case in which the types of mobile phases contained in the streams are different from each other includes, for example, a case in which Type 1 and Type 2 mobile phases are used in stream 60A, and only Type 1 mobile phase is used in stream 60B. Note that in this example, stream 60B has only one mobile phase container, so a mixer is not required. The stream configuration may also include, for example, the type of column. In other words, the types of columns contained in each stream may be different from each other. "Different column types" includes, for example, differences in at least one of the column packing material type, its chemical modification, particle size, length, and diameter. Users can use different column types depending on the application. The column is, for example, a C18 column or a C8 column. The C18 column and the C8 column are reverse-phase HPLC columns containing different types of packing materials.

[0031] The controller 110 can switch between the streams 60A-60D used to separate the samples, thereby allowing the LC system 10 to analyze various samples sequentially in the detector 500.

[0032] In particular, because the stream configurations of streams 60A-60D are different, samples with different sample separation conditions (referred to herein as "analysis conditions") can be analyzed using a single analysis system. The analysis conditions are, for example, at least one of the type of mobile phase supplied to the column and the type of column. The analysis conditions may also include other conditions, such as the column temperature control temperature and the mobile phase mixing ratio. Each analysis condition may be stored and managed by the control device 110, or may be managed by the user without being stored in the control device 110. The control device 110 or the user selects a stream from streams 60A-60D that has a stream configuration corresponding to the sample analysis conditions. This allows analysis to be performed under analysis conditions appropriate for each sample. Furthermore, the control device 110 can separate samples in multiple streams in parallel and analyze each of the separated samples using the detector 500 at different time periods. In this way, the control device 110 can efficiently use each stream by appropriately allocating the separation of each sample to each stream.

[0033] 4 is a diagram illustrating the configuration of the control device 110. The control device 110 includes a main body 111, an input device 14, and a display device 15.

[0034] The main body 111 includes a processor 11, a memory 12, and an input / output interface (I / F) 13. The processor 11 is typically an arithmetic processing unit such as a CPU (Central Processing Unit) or an MPU (Multi-Processing Unit). The processor 11 reads and executes programs stored in the memory 12 to realize processing of the LC system.

[0035] The memory 12 is realized by a nonvolatile memory such as a random access memory (RAM), a read-only memory (ROM), or a flash memory. The memory 12 may be configured by a compact disc read-only memory (CD-ROM), a digital versatile disc read-only memory (DVD-ROM), a universal serial bus (USB) memory, a memory card, a flexible disc (D), a hard disk, a solid state drive (SSD), a magnetic tape, a cassette tape, a magnetic optical disc (MO), a mini disc (MD), an integrated circuit (IC) card (excluding a memory card), an optical card, a mask ROM, or an EPROM, as long as the memory 12 can non-temporarily record a program in a format readable by the processor 11. The memory 12 stores analytical conditions for separating each sample for each sample.

[0036] The input / output I / F 13 is an interface for exchanging various types of data between the processor 11 and external devices connected to the input / output I / F 13. The external devices include an input device 14 and a display device 15.

[0037] The input device 14 is configured by, for example, a keyboard and a mouse. A user can input various instructions to the control device 110 by operating the input device 14.

[0038] The display device 15 displays an image according to the video signal output by the control device 110. The display device 15 is, for example, a display.

[0039] [4. Comparison with Conventional Liquid Chromatography Systems] Patent Document 1 and Non-Patent Document 1 disclose an analytical system including an autosampler that performs pre-treatment and post-treatment of a sample, multiple streams that separate components present in the pre-treated sample, and a mass spectrometer that detects the separated components. Such an analytical system is also called a multiplex LC-MS (Liquid Chromatography-Mass spectrometry) system.

[0040] According to the analytical systems described in Patent Document 1 and Non-Patent Document 1, throughput can be improved by separating multiple samples in parallel using multiple streams. However, even if there are multiple streams, if the configurations related to analytical conditions for sample separation (stream configurations), such as columns and mobile phases incorporated in each of the multiple streams, are the same, it may not be possible to analyze samples having different analytical conditions using a single analytical system.

[0041] In consideration of the above circumstances, the LC system 10 according to the present embodiment includes multiple streams with different stream configurations related to the analysis conditions of the samples. This makes it possible to analyze samples with different analysis conditions, such as samples using different types of columns for separation, in a single system. In other words, the LC system 10 is a system that can appropriately handle multiple analysis conditions (multi-methods).

[0042] The LC system 10 also differs from conventional analytical systems in that all streams have the same stream configuration in the way it assigns analyses to each stream. In conventional analytical systems, the same streams are naturally available for all analyses. In contrast, the LC system 10 supports different analytical conditions for each stream. Therefore, in the LC system 10, the control device 110 performs processing to appropriately assign analyses to each stream according to predetermined criteria. Furthermore, in the LC system 10, the user can select the criteria according to their own purpose.

[0043] 5. Allocation of Analysis in LC System According to Embodiment Next, allocation of analysis in the LC system 10 will be described using a specific example.

[0044] (5-1. How to Determine Analytical Conditions that Can Be Implemented in Each Stream) First, how the control device 110 determines analytical conditions that can be implemented in each stream will be described.

[0045] In this example, the LC system 10 includes streams 1 to 4. Each stream has a different stream configuration (combination of column type and mobile phase type).

[0046] For example, in stream 1, a type 1 column is installed, and three mobile phase containers are filled with mobile phases of types 1, 2, and 3. On the other hand, in stream 2, a type 2 column is installed, and four mobile phase containers are filled with mobile phases of types 1, 2, 3, and 4.

[0047] Table 1 below shows the stream configurations of streams 1 to 4, including the above-mentioned streams 1 and 2, in this example.

[0048]

[0049] Next, consider a case where the following four types of analyses are performed under different analytical conditions in the LC system 10 including the above-mentioned streams 1 to 4.

[0050] For example, analysis condition 1 is a condition in which a type 1 column is used and a mixture of type 1 and type 2 mobile phases is used.

[0051] Table 2 below shows a summary of the four analytical conditions in this example, including the analytical condition 1 described above.

[0052]

[0053] The control device 110 acquires the unit configurations of streams 1 to 4 and the analytical conditions 1 to 4. Then, it compares the unit configurations of streams 1 to 4 with the analytical conditions 1 to 4 to determine the analytical conditions that can be implemented for each stream. Specifically, if the stream configuration of a given stream satisfies a given analytical condition, the control device 110 determines that the given analytical condition can be implemented for that given stream. In this specification, the ability of a given analytical condition to be implemented in a given stream configuration is also referred to as "the analytical condition corresponds to the stream configuration."

[0054] Based on the above, the analytical conditions that can be implemented in each stream are as shown in Table 3 below.

[0055]

[0056] The control device 110 assigns analyses to each stream according to predetermined criteria, described below, based on the analytical conditions that can be performed in each determined stream. For example, if stream 1 is a stream in which no analysis is currently being performed (hereinafter also referred to as an "empty stream"), the control device 110 assigns analysis under analytical condition 1 or analytical condition 2. With this configuration, when the previous analysis in a stream is completed and the stream becomes empty, it is possible to search for and start an analysis that can be performed in that empty stream. This allows streams to be used effectively, improving the throughput of the analysis device 100.

[0057] The process of assigning executable analysis conditions to each stream may be performed by the control device 110 as in the above example, or the user may input and store the assignments they have devised into the control device 110.

[0058] (5-2. Management of Sample Information for Each Batch Analysis) In one embodiment, analyses are managed as a group of multiple analyses called a batch analysis. Each of the multiple analyses in a batch analysis is typically assigned an analysis number for management purposes. A batch analysis is typically an analysis in which at least one of the analysis-related elements, such as the collection date and time, the purpose of the analysis, the person performing the analysis, or the person requesting the analysis, is the same. By managing these related analyses together as a batch analysis, analysis management becomes easier than managing each individual analysis separately. In this specification, all of the batch analyses performed at one time in the LC system 10 will be referred to as "all analyses."

[0059] The control device 110 stores sample information associated with each analysis included in the batch analysis. The sample information includes at least the plate number, sample number, and analysis conditions corresponding to the sample. The sample information may also include other information such as the sample name, sample ID, collection date and time, analysis purpose, analysis performer, and analysis requester.

[0060] The plate number, sample number, and analysis conditions for each analysis included in the batch analysis are registered in the LC system 10, for example, as shown in Table 4 below. Hereinafter, the batch analysis number will be referred to as the "batch number." Furthermore, the analysis with analysis number 1 in the batch analysis with batch number 1 will be referred to as analysis 1-1. The same analysis conditions are often set for multiple analyses within a batch analysis, but different analysis conditions may also be set for each analysis, as shown in the example of Table 4.

[0061] Table 4 below shows the plate numbers, sample numbers, and analysis conditions for the samples corresponding to each analysis included in batch analyses 1 and 2.

[0062]

[0063] Referring to Table 4, for example, analysis 1-1 is an analysis of sample number 1 on plate number 1, and the analysis conditions are 1.

[0064] The control device 110 acquires the plate number, sample number, analysis conditions, etc. from the sample information registered for each analysis as described above. Then, as will be described later, the control device 110 assigns the analysis to a stream corresponding to the analysis conditions based on predetermined criteria based on the analysis number, plate number, etc. As described above, the control device 110 can select an appropriate stream and perform the analysis even when there are analyses with different analysis conditions among analyses managed in a batch format.

[0065] (5-3. Criteria for Allocating Analysis) In the LC system 10, the user can select criteria for allocating the above-mentioned analysis according to his / her own purpose. The selection of the criteria is performed using the input device 14, for example.

[0066] (5-3-1. Batch Criteria) For example, if a user desires to perform analysis for each batch analysis, the control device 110 performs all analyses of one batch analysis, and then performs all analyses of the next batch analysis. In other words, the control device 110 selects an unperformed analysis among the batch analyses that includes the currently-performed analysis as a candidate for the next analysis. For example, in the above example, after analyses 1 to 4 of batch analysis 1 are performed, analyses 1 to 4 of batch analysis 2 are performed. Such criteria are referred to as "batch criteria" in this specification. The batch criteria correspond to an example of the "second criteria."

[0067] When analysis is performed on a batch basis, it is easy for the user to understand which batch analysis has been completed.

[0068] Furthermore, as mentioned above, batch analyses often share common analysis-related elements such as the analysis date and time, the purpose of the analysis, the person performing the analysis, and the person requesting the analysis, so all of the analysis results within a batch analysis are often used together. Therefore, there is an advantage in that the analysis results of a given batch analysis can be used as soon as all of the analyses within that batch analysis are completed, without having to wait for the analysis of all of the batch analyses to be completed. In other words, the results can be used sequentially as soon as the analysis of each batch analysis unit is completed.

[0069] In one embodiment, the control device 110 performs analyses within a batch analysis in ascending order of analysis number. This has the advantage of allowing the user to easily understand how far the analysis has progressed within the batch analysis. However, the control device 110 may also perform analyses within the currently running batch analysis, starting with analyses having analysis conditions corresponding to empty streams, regardless of the analysis number. In this case, streams can be used more effectively than when analyses are performed in order of analysis number within the batch analysis. For example, if analyses have been performed up to analysis 2-1 in the above example, and the stream corresponding to analysis condition 3 becomes an empty stream, analysis 2-2 can be skipped and analysis 2-3 can be performed.

[0070] (5-3-2. Plate Criteria) When a user desires to analyze each sample plate 80, the control device 110 analyzes all samples contained in one sample plate 80, and then analyzes all samples contained in the next sample plate 80. In other words, the control device 110 selects an unperformed analysis among the sample plates that contained samples for the current analysis as a candidate for the next analysis. For example, in the above example, analyses 1-1, 1-2, 2-1, and 2-2 contained in the sample plate 80 with plate number 1 are performed, and then analyses 1-3 and 1-4 contained in the sample plate 80 with plate number 2 are performed. Such a criterion is referred to as a "plate criterion" in this specification. The plate criterion corresponds to an example of the "third criterion."

[0071] When analysis is performed on a plate basis, it is easy for the user to understand up to which sample plate 80 analysis has been completed.

[0072] Similarly to batch analysis, sample plates 80 often share common analytical elements, such as the analysis date and time, analytical purpose, analyst, and analyst requester. For example, a series of samples with a specific analytical purpose may be stored sequentially on plate number 1 for each day the samples were obtained, while a series of samples with a different analytical purpose may be stored sequentially on plate number 2 for each day the samples were obtained. In this manner, at least some of the analytical results of samples stored on the same sample plate 80 are often used together. This has the advantage that, once the analysis of all samples on a given sample plate 80 has been completed, the analytical results of the samples on that given sample plate 80 can be used without waiting for the analysis of all sample plates 80 to be completed. In other words, once the analysis of each sample plate 80 has been completed, the results can be used sequentially.

[0073] In one embodiment, the control device 110 performs analysis on the sample plate 80 in ascending order of sample number. This has the advantage that the user can easily see up to which sample number the analysis has progressed on the sample plate 80. However, the control device 110 may perform analysis on the sample plate 80 containing a sample currently being analyzed, starting with the sample under the analysis conditions corresponding to an empty stream, regardless of the sample number. In this case, the streams can be used more effectively than when the analysis is performed on the sample plate 80 in order of sample number.

[0074] (5-3-3. Stream Criteria) When the user places the greatest importance on the throughput of the LC system 10 as a whole, the control device 110 searches for an analysis that can be performed in an empty stream from among the unperformed analyses included in all batch analyses, and performs the analysis in order to increase the drive rate of each stream. In other words, the control device 110 selects an unperformed analysis from among the analyses included in the batch analysis for each batch analysis, and sets it as a candidate for the next analysis. Such a criterion that emphasizes the drive rate of a stream is referred to in this specification as the "stream criterion." The stream criterion corresponds to an example of the "first criterion." When analysis is performed on a stream basis, the time required to perform all analyses can be further reduced compared to both the batch criterion and the plate criterion.

[0075] In one embodiment, the control device 110 selects the analysis with the smallest analysis number among the unperformed analyses in each batch analysis as a candidate for analysis to be performed in an empty stream. Then, from the selected candidates, the analysis to be performed in the empty stream is determined. In this case, the analyses proceed in order of analysis number within each batch analysis, which has the advantage that it is easy for the user to see how far the analysis has progressed within the batch analysis. However, the control device 110 may also select an analysis having analysis conditions corresponding to an empty stream within each batch analysis as a candidate for analysis, regardless of analysis number. In this case, streams can be used more effectively than when analyses are performed in order of analysis number within each batch analysis.

[0076] (5-4. User-specified analysis priority) In addition to the criteria exemplified above, the control device 110 may have a function to prioritize the execution of an analysis specified by the user. For example, if there is an analysis for which the user wants to check the analysis results earlier than other analyses, the user specifies that analysis as a "priority analysis" that is to be prioritized. Priorities can be assigned among the priority analyses, and the control device 110 may be configured to execute the priority analyses in the order of the priorities.

[0077] (5-5. Summary) As described above, among the unperformed analyses, an analysis that satisfies the predetermined criteria is selected as a candidate for the next analysis. Then, from among these candidates, an analysis having analysis conditions corresponding to the stream configuration of the empty stream is determined as the next analysis. This allows the analysis to proceed in an analysis order that reflects the user's needs. Furthermore, by configuring the system so that the user can select which of the predetermined criteria to adopt using the input device 14, the user can reselect the criteria each time they use the LC system 10. Specifically, if the user places the highest priority on throughput, the user can select the stream criteria; if the user wishes to perform analysis in batches, the user can select the batch criteria; and if the user wishes to perform analysis in units of sample plates 80, the user can select the plate criteria. This configuration allows the user to proceed with the analysis in a convenient analysis order that suits the user, based on the purpose of the analysis, and other circumstances. Furthermore, it is also possible to perform a priority analysis specified by the user first. This allows the LC system 10 to perform analysis that meets the user's needs.

[0078] 6. Flowchart Next, the processing of the control device 110 for realizing the analysis allocation described above will be described using a flowchart.

[0079] (6-1. Analysis Allocation Processing Based on Analysis Conditions and Stream Configuration) FIG. 5 is a flowchart showing processing related to control of the liquid chromatograph system according to this embodiment.

[0080] In step (hereinafter referred to as "S"), the control device 110 acquires criteria for analysis allocation selected by the input device 14. According to one embodiment, the user uses the input device 14 to select one of three criteria, "stream criteria," "batch criteria," and "plate criteria," which are displayed on the display device 15. The input device 14 then transmits the criteria selected by the user to the control device 110.

[0081] In S04, a specific batch analysis is performed with analysis number 1. The specific batch analysis may be, for example, the batch analysis with batch number 1, or may be a batch analysis selected by the user.

[0082] In S06, the control device 110 selects an analysis that satisfies a predetermined criterion from among the unperformed analyses as a candidate for the next analysis.

[0083] In S08, the control device 110 determines whether there is an empty stream that is not currently performing analysis among the multiple streams included in the LC system 10. According to one embodiment, each stream transmits a signal indicating whether it is an empty stream to the control device 110. Then, the control device 110 determines whether each stream is an empty stream based on the signal.

[0084] If there is no free stream (NO in S08), control device 110 returns the process to S08.

[0085] If there is an empty stream (YES in S08), in S10 the control device 110 acquires information indicating the stream configuration of the empty stream. In one embodiment, information indicating the stream configuration of each stream is stored in the memory 12 of the control device 110. Then, the control device 110 acquires information indicating the stream configuration of the empty stream from the information.

[0086] In S12, the control device 110 selects, from the candidates selected in S06, an analysis having analysis conditions corresponding to the stream configuration of the empty stream as the next analysis.

[0087] In S14, the control device 110 performs the analysis determined in S12 on the empty stream.

[0088] In S16, the control device 110 determines whether or not all analyses have been performed. If all analyses have been performed (YES in S16), the control device 110 ends the process.

[0089] If there are any unperformed analyses remaining (NO in S16), controller 110 returns the process to S06.

[0090] The process of FIG. 5 allows samples with different analytical conditions to be efficiently analyzed using a liquid chromatographic system including multiple streams, according to user-selected criteria.

[0091] (6-2. Stream-Based Analysis Allocation Processing) FIG. 6 is a flowchart for explaining the stream-based analysis allocation processing.

[0092] In Fig. 6, S06 in Fig. 5 is replaced by S06A. The other steps in Fig. 6 are the same as those in Fig. 5, and therefore description thereof will be omitted.

[0093] In S06A, the control device 110 selects, for each batch analysis, an analysis that has not been performed and has the smallest analysis number from among the analyses included in the batch analysis, and selects it as a candidate for the next analysis.

[0094] According to the process of FIG. 6, streams can be operated efficiently, and samples with different analysis conditions can be analyzed efficiently using a liquid chromatograph system including multiple streams.

[0095] (6-3. Batch-Based Analysis Allocation Processing) Fig. 7 is a flowchart for explaining batch-based analysis allocation processing. In the processing of Fig. 7, sample analysis proceeds for each batch analysis.

[0096] In Fig. 7, S06 and S16 in Fig. 5 are replaced with S06B and S16B, respectively. Furthermore, S18B to S20B are added. The other steps in Fig. 7 are the same as those in Fig. 5, so their explanation will be omitted.

[0097] In S06B, the control device 110 selects, as a candidate for the next analysis, an analysis that has not yet been performed and has the smallest analysis number among the batch analyses that include an analysis currently being performed in at least one of the multiple streams.

[0098] In S16B, the control device 110 determines whether all analyses in the ongoing batch analysis have been performed.

[0099] If the ongoing batch analysis includes an analysis that has not yet been performed (NO in S16B), controller 110 returns the process to S06B.

[0100] When all of the ongoing batch analyses have been completed (YES in S16B), in S18B, the control device 110 determines whether there are any unperformed batch analyses. Note that an "unperformed batch analysis" refers to a batch analysis in which all of the analyses included in the batch analysis have not yet been performed.

[0101] If all batch analyses are completed (NO in S18B), the control device 110 ends the process.

[0102] If there are any unperformed batch analyses (YES in S18B), in S20B, the control device 110 performs the analysis of analysis number 1 of one of the unperformed batch analyses, and then returns the process to S06B. An example of this batch analysis is a batch analysis whose batch number is one higher than the batch analysis performed immediately before. This has the advantage that the user can easily grasp the progress of all analyses. Another example of the above-mentioned batch analysis is a batch analysis in which analysis number 1 can be performed in a stream that is expected to become the next free stream. The stream that is expected to become the next free stream is, for example, a stream whose analysis is expected to be completed soon. In this case, the stream can be used more effectively than if the analyses were performed in batch number order. Another example of the above-mentioned batch analysis is a batch analysis determined by the batch analysis order specified in advance by the user. In this case, the batch analysis for which the user wants results quickly can be executed with priority.

[0103] 7, samples with different analytical conditions can be efficiently analyzed using a liquid chromatograph system including multiple streams. Furthermore, the analysis can be carried out in batch analysis units.

[0104] (6-4. Plate-Based Analysis Allocation Processing) Fig. 8 is a flowchart for explaining plate-based analysis allocation processing. In the processing of Fig. 8, sample analysis proceeds for each sample plate.

[0105] In Fig. 8, S06 and S16 in Fig. 5 are replaced with S06C and S16C, respectively. Also, S18C to S20C are added. The other steps in Fig. 8 are the same as those in Fig. 5, so their explanation will be omitted.

[0106] In S06C, the control device 110 selects the analysis with the smallest analysis number that has not yet been performed among the sample plates 80 that contain samples for analyses currently being performed in at least one of the multiple streams as a candidate for the next analysis.

[0107] In S16C, the control device 110 determines whether or not all samples on the sample plate under analysis have been analyzed.

[0108] If the sample plate being analyzed contains samples for which analysis has not yet been performed (NO in S16B), the controller 110 returns the process to S06C.

[0109] When the analysis of all samples on the sample plate under analysis has been completed (YES in S16C), the control device 110 determines in S18C whether there is any sample plate for which analysis has not been performed. Note that an "unperformed sample plate" refers to a batch analysis in which all of the analyses corresponding to the samples contained on the sample plate have not been performed.

[0110] If the analysis has been completed for all sample plates (NO in S18C), the control device 110 ends the process.

[0111] If there are sample plates that have not yet been analyzed (YES in S18C), in S20C, the control device 110 analyzes sample number 1 of one of the sample plates, and then returns the process to S06C. An example of this sample plate is a sample plate whose plate number is one higher than the sample plate that was previously analyzed. This has the advantage that the user can easily grasp the progress of all analyses. Another example of this sample plate is a sample plate that can be analyzed for sample number 1 in the stream that is expected to be the next free stream. In this case, the stream can be used more effectively than if the analyses were performed in order of plate number. Another example of this sample plate is a sample plate determined by the analysis order of sample plates previously specified by the user. In this case, the analysis of the sample plate for which the user wants results quickly can be performed with priority.

[0112] 8, samples with different analytical conditions can be efficiently analyzed using a liquid chromatograph system including multiple streams. Furthermore, analysis can be performed on a sample plate-by-sample basis.

[0113] (6-5. Priority Analysis Implementation Process) FIG. 9 is a flowchart for explaining priority analysis implementation process.

[0114] In Fig. 9, steps S011D and S012D are added before step S02 in Fig. 5. The other steps are the same as in Fig. 5, and therefore the description thereof will be omitted.

[0115] In S011D, the control device 110 determines whether input of a priority analysis has been received. In one embodiment, the user uses the input device 14 to select an analysis that the user wants to prioritize from among the analyses displayed on the display device 15. The input device 14 then transmits information about the analysis selected by the user to the control device 110.

[0116] If an input of an analysis to be prioritized is received (YES in S011), in S012D, control device 110 performs the selected analysis as the priority analysis before other analyses.

[0117] If input of the analysis to be prioritized has not been received (NO in S011), control device 110 advances the process to S02.

[0118] The process of FIG. 9 makes it possible to efficiently analyze samples with different analytical conditions using a liquid chromatograph system including multiple streams, and also makes it possible to perform a prioritized analysis selected by the user first.

[0119] 7. Display of Analysis Progress As described above, the LC system 10 performs all analyses according to a user-selected standard from among multiple standards. Therefore, the order in which the analyses are performed may vary depending on the standard. In particular, when all analyses are performed according to a stream standard, each batch analysis is performed in parallel, making it difficult for the user to understand the progress of each batch.

[0120] Therefore, in the LC system 10, the progress of each batch analysis is displayed on the display device 15 to help the user understand the situation.

[0121] 10 is an example of a display screen 151 showing the progress of all analyses. The display screen 151 is displayed on the display device 15.

[0122] 10, the display screen 151 includes a table Tb1 and a table Tb2. Table Tb1 displays information on analyses that have not yet been performed. In the example of FIG. 10, table Tb1 also displays information on analyses in preparation and analyses currently being performed. "In preparation" refers to, for example, a state in which, in a stream where the previous analysis has been completed, the next sample to be analyzed has been determined and preparations for the next analysis are being made.

[0123] Table Tb1 displays each analysis for each batch analysis and displays information about each analysis. Specifically, Table Tb1 includes items such as "Batch Number," "Plate Number," "Sample Number," "Sample Name," "Analysis Conditions," "Stream," and "Status." The column corresponding to each item displays the corresponding value. For example, the value corresponding to the "Status" item is either "In Preparation," "In Progress," or "Not Yet Performed."

[0124] Table Tb2 is a table that displays information on completed analyses. In the example of Fig. 10, table Tb1 also displays information on analyses being prepared and analyses being performed.

[0125] Table Tb2 displays the analyses, including preparation, in the order in which they were performed, and displays information about the analyses. Table Tb2 includes the same items as Table Tb1, as well as an item for the "order in which the analyses were performed." The corresponding column displays a corresponding value. However, the value for the "Status" item in Table Tb2 is either "In preparation," "In progress," or "Completed."

[0126] By using the display screen 151, the user can easily check the incomplete analyses in the stream by looking at the table Tb1. Furthermore, the user can easily check whether each analysis included in the incomplete analyses is an analysis that has not been performed, an analysis that is in preparation, or an analysis that is being performed.

[0127] In particular, Table Tb1 displays the analyses by batch analysis, making it easy to understand the progress of each batch analysis. For example, it is easy to understand how many analyses have been completed and / or what percentage of the analyses remain for each batch analysis. Furthermore, batch analyses in which all analyses have been completed are not displayed in Table Tb1, so by checking the batch number column in Table Tb1, it is easy to find batch analyses in which all analyses have been completed.

[0128] Also, by visually checking the method of analysis of the pending status, it is possible to infer the stream to be used.

[0129] It is also possible to find a sample plate 80 on which all samples have been run.

[0130] On the other hand, by looking at Table Tb2, the user can confirm the analyses for which the start of an analysis including preparation has already been performed in the stream. Furthermore, the user can easily confirm whether each analysis included in the analyses for which the start of the analysis has already been performed is an analysis in preparation, an analysis in progress, or an analysis that has been completed.

[0131] Table Tb2 also allows the user to see the order in which these analyses were performed, i.e., which batch analyses were performed early and which batch analyses are progressing slowly.

[0132] It is also possible to find a batch analysis where all analyses have been completed, and a sample plate 80 where all samples have been analyzed.

[0133] As described above, the display screen 151 allows the user to intuitively grasp the progress of all analyses. Specifically, the user can easily read multiple important pieces of information related to the analyses from two simple tables. This reduces inconvenience and stress caused by the user having difficulty understanding the progress of all analyses or each batch analysis. For example, the user can easily grasp the progress of all analyses and wait for the completion of all analyses while recognizing that the progress is going smoothly. This also makes it easier for the user to perform other tasks in parallel while the LC system 10 is performing all analyses. Furthermore, because the overall analysis is easily visible, it is also easier to plan other tasks to be performed after the completion of the all analyses.

[0134] In particular, even when multiple batch analyses are being performed in parallel using stream-based and plate-based analysis, users can easily grasp the progress of each batch analysis. This improves work efficiency by allowing users to review the analysis results of completed batches without waiting for all analyses to be completed.

[0135] In addition, the display screen 151 may also include other information useful to the user, such as the name of the criteria selected by the user, the status of each stream (e.g., status such as analysis in progress, analysis preparation, empty stream, etc.), and an estimate of the time required for the entire analysis calculated by the control device 110.

[0136] Aspects It will be understood by those skilled in the art that the exemplary embodiments described above are examples of the following aspects.

[0137] (Item 1) A liquid chromatograph system according to one aspect is a liquid chromatograph system for analyzing multiple samples. The liquid chromatograph system includes streams, a sample injection device, a detector, and a control device. The multiple streams separate samples. The sample injection device injects a sample into each of the multiple streams. The detector is disposed downstream of the multiple streams and analyzes the sample separated in each of the multiple streams. The control device stores analytical conditions for separating the sample for each sample. Each of the multiple streams includes a column and a supply device. The column separates the sample injected into the stream. The supply device supplies a mobile phase for use in the column. The multiple streams include at least two streams having different stream configurations for sample separation. The control device selects a stream from the multiple streams having a stream configuration that corresponds to the analytical conditions for the sample.

[0138] According to the liquid chromatograph system described in paragraph 1, even when analyzing samples with different analytical conditions, a stream capable of implementing each analytical condition can be selected and executed. Therefore, samples with different analytical conditions can be efficiently analyzed using a liquid chromatograph system including multiple streams.

[0139] (Item 2) In the liquid chromatograph system described in item 1, the analysis conditions are at least one of the type of mobile phase supplied to the column and the type of column.

[0140] According to the liquid chromatograph system described in paragraph 2, samples that differ in analytical conditions, such as the type of mobile phase and the type of column, can be efficiently analyzed using a liquid chromatograph system including multiple streams.

[0141] (Item 3) In the liquid chromatography system described in Items 1 or 2, the supply device includes a container and a pump. The container contains a predetermined type of mobile phase. The pump supplies the mobile phase contained in the container to the column. The stream configuration includes at least one of the type of mobile phase contained in the container and the type of column.

[0142] According to the liquid chromatograph system described in paragraph 3, samples that differ in analytical conditions, such as the type of mobile phase and the type of column, can be efficiently analyzed using a liquid chromatograph system including multiple streams.

[0143] (4) In the liquid chromatograph system described in any one of paragraphs 1 to 3, when at least one of the multiple streams is an empty stream in which no analysis is being performed, the control device determines an analysis whose analysis conditions correspond to the stream configuration of the empty stream as the next analysis to be performed in the empty stream.

[0144] According to the liquid chromatograph system described in paragraph 4, when a stream has completed a previous analysis and is now empty, it is possible to search for and start an analysis that can be performed in that empty stream. This allows the stream to be used efficiently, thereby improving the throughput of the analytical system.

[0145] (5) In the liquid chromatograph system described in 4, the control device selects, from among the unperformed analyses, an analysis that satisfies a predetermined criterion as a candidate for the next analysis. Then, the control device determines, from among the candidates, an analysis having analysis conditions that correspond to the stream configuration of the empty stream as the next analysis.

[0146] According to the liquid chromatograph system described in paragraph 5, the analysis can be carried out in an analysis order that reflects the needs of the user.

[0147] (Item 6) In the liquid chromatograph system described in Item 5, the analysis of the plurality of samples includes one or more batch analyses. The batch analysis includes a plurality of analyses each having an analysis number.

[0148] According to the liquid chromatograph system described in paragraph 6, the control device can select an appropriate stream and perform the analysis even when there are analyses with different analytical conditions among analyses managed in a batch format.

[0149] (Item 7) In the liquid chromatograph system described in items 5 or 6, the predetermined criterion is a first criterion that an unperformed analysis among the analyses included in the batch analysis is selected for each batch analysis and made a candidate.

[0150] According to the liquid chromatography system described in Section 7, the time required to perform the entire analysis can be further reduced compared to each of the second and third criteria described below.

[0151] (Item 8) In the liquid chromatograph system described in item 5 or 6, the predetermined criterion is a second criterion that an unperformed analysis is selected as a candidate among batch analyses that include an analysis currently being performed.

[0152] According to the liquid chromatographic system described in paragraph 8, the results of the analysis of each batch analysis unit can be used as soon as they are completed.

[0153] (Item 9) The liquid chromatograph system according to item 5 or 6 further comprises one or more sample plates each containing one or more samples. The predetermined criterion is a third criterion that an analysis that has not yet been performed among the sample plates containing samples for the analysis currently being performed is to be selected as a candidate for the analysis.

[0154] According to the liquid chromatographic system described in paragraph 9, the results can be used as soon as the analysis of each sample plate is completed.

[0155] (Item 10) In the liquid chromatograph system according to any one of items 5 to 9, the control device includes an input device that allows selection of an analysis of a sample. The control device performs the analysis selected using the input device before other analyses.

[0156] According to the liquid chromatograph system described in the tenth aspect, the analysis that is to be prioritized and selected by the user can be carried out first.

[0157] (Item 11) In the liquid chromatograph system according to any one of items 5 to 10, the control device includes an input device, and the predetermined criteria are criteria selected by a user using the input device.

[0158] According to the liquid chromatograph system described in paragraph 11, the user can easily carry out the analysis in an analysis order that is convenient for the user, according to the circumstances such as the purpose of the analysis.

[0159] (Item 12) In the liquid chromatograph system described in item 6, the control device further includes a display device that displays the progress of each batch analysis.

[0160] According to the liquid chromatography system described in paragraph 12, the user can easily understand the progress of the entire analysis or each batch analysis, thereby reducing inconvenience and stress caused to the user due to difficulty in understanding the progress of the entire analysis or each batch analysis.

[0161] (Item 13) A control method according to another aspect is a computer-implemented control method for a liquid chromatograph system for analyzing multiple samples. The liquid chromatograph system includes streams, a sample injection device, and a detector. The multiple streams separate samples. The sample injection device injects a sample into each of the multiple streams. The detector is disposed downstream of the multiple streams and analyzes the sample separated in each of the multiple streams. Each of the multiple streams includes a column and a supply device. The column separates the sample injected into the stream. The supply device supplies a mobile phase for use in the column. The multiple streams include at least two streams having different stream configurations for sample separation. The control method includes the steps of selecting, from the multiple streams, a stream having a stream configuration corresponding to analytical conditions for sample separation, and performing sample analysis in the selected stream.

[0162] According to the method described in paragraph 13, even when analyzing samples with different analytical conditions, a stream capable of implementing each analytical condition can be selected and executed. Therefore, samples with different analytical conditions can be efficiently analyzed using a liquid chromatograph system including multiple streams.

[0163] The embodiments disclosed herein should be considered to be illustrative in all respects and not restrictive. The scope of the present invention is defined by the claims, not by the description of the above embodiments, and is intended to include all modifications within the meaning and scope of the claims.

[0164] 60A, 60B, 60C, 60D stream, 70A column, 10 LC system, 11 processor, 12 memory, 13 input / output I / F, 14 input device, 15 display device, 18 sample injection device, 20A mobile phase container, 30A mixer, 180A valve, 40A mobile phase pump, 50A supply device, 80 sample plate, 90 divert valve, 91, 92, 93, 94, 95, 96 port, 100 analyzer, 110 control device, 111 main body, 151 display screen, 292 detector flow path, 500 detector.

Claims

1. 1. A liquid chromatographic system for performing analysis of a plurality of samples, comprising: The liquid chromatography system comprises: multiple streams for separating samples; a sample injection device for injecting a sample into each of the plurality of streams; a detector disposed downstream of the plurality of streams for analyzing separated samples in each of the plurality of streams; a control device that stores analytical conditions for separating the sample for each sample; Each of the plurality of streams comprises: a column for separating a sample injected into said stream; a supply device for supplying a mobile phase for use in the column; The supply device comprises: a container containing a predetermined type of mobile phase; a pump for supplying the mobile phase contained in the container to the column; the plurality of streams include at least two streams that are included in a stream configuration related to sample separation and have different combinations of the type of mobile phase contained in the container and the type of the column; The control device selects from the plurality of streams a stream having a stream configuration corresponding to a combination of the type of mobile phase supplied to the column and the type of the column, which is a sample analysis condition.

2. The control device 2. The liquid chromatograph system of claim 1, wherein, when at least one of the plurality of streams is an empty stream in which no analysis is being performed, an analysis whose analysis conditions correspond to the stream configuration of the empty stream is determined as the next analysis to be performed in the empty stream.

3. The control device Selecting an analysis that satisfies a predetermined criterion from among the unperformed analyses as a candidate for the next analysis; 3. The liquid chromatograph system according to claim 2, wherein an analysis having analysis conditions corresponding to the stream configuration of the empty stream is determined as the next analysis from among the candidates.

4. the analysis of the plurality of samples comprises one or more batch analyses; The liquid chromatographic system of claim 3 , wherein the batch analysis comprises a plurality of analyses each having an analysis number.

5. 4. The liquid chromatograph system according to claim 3, wherein the predetermined criterion is a first criterion that an unexecuted analysis is selected for each batch analysis from among the analyses included in the batch analysis and set as the candidate.

6. 4. The liquid chromatograph system according to claim 3, wherein the predetermined criterion is a second criterion that an analysis that has not yet been performed among a batch analysis that includes an analysis that is currently being performed is selected as the candidate.

7. further comprising one or more sample plates each containing one or more samples; 4. The liquid chromatograph system according to claim 3, wherein the predetermined criterion is a third criterion that the candidate is an analysis that has not yet been performed among sample plates that contain samples for an analysis currently being performed.

8. the control device includes an input device capable of selecting an analysis of the sample; 4. The liquid chromatograph system according to claim 3, wherein the predetermined criterion is a fourth criterion that an analysis selected using the input device is analyzed before other analyses.

9. the control device includes an input device; 4. The liquid chromatograph system of claim 3, wherein the predetermined criteria are selected by a user using the input device.

10. The liquid chromatograph system according to claim 4 , wherein the control device further includes a display device that displays the progress of each batch analysis.

11. 1. A computer-implemented method for controlling a liquid chromatographic system for performing analysis of a plurality of samples, comprising: The liquid chromatography system comprises: multiple streams for separating samples; a sample injection device for injecting a sample into each of the plurality of streams; a detector disposed downstream of the plurality of streams and configured to analyze the separated samples in each of the plurality of streams; Each of the plurality of streams comprises: a column for separating a sample injected into said stream; a supply device for supplying a mobile phase for use in the column; The supply device comprises: a container containing a predetermined type of mobile phase; a pump for supplying the mobile phase contained in the container to the column; the plurality of streams include at least two streams that are included in a stream configuration related to sample separation and have different combinations of the type of mobile phase contained in the container and the type of the column; The control method includes: selecting, from the plurality of streams, a stream having a stream configuration corresponding to a combination of the type of mobile phase supplied to the column and the type of the column, which is an analytical condition for separating a sample; and performing an analysis of a sample in the selected stream.