Screening Methods

Claims

1. A screening method for evaluating whether a plurality of candidate compounds can be used as a plant biostimulant, a plant disease prevention agent, or a plant herbicide, comprising: a primary screening step of contacting plant cells with the candidate compounds, culturing the cells under oxidative stress-inducing conditions and under oxidative stress-non-inducing conditions, measuring the amount of reactive oxygen species produced, and selecting a primary candidate compound from the candidate compounds; a secondary screening step of contacting the plant cells with the primary candidate compounds, then culturing them under oxidative stress-inducing conditions and under oxidative stress-non-inducing conditions, and measuring the viability of the plant cells to perform secondary screening, thereby selecting secondary candidate compounds from the primary candidate compounds.

2. The plant cells are photosynthetic plant cells, 2. The screening method according to claim 1, wherein the viability of the plant cells is determined by measuring autofluorescence derived from chlorophyll in the secondary screening step.

3. 3. The method of claim 2, wherein the plant cells are T-87 strain green cultured cells derived from Arabidopsis thaliana.

4. 2. The screening method according to claim 1, wherein the substance used under the oxidative stress-inducing conditions is paraquat (methyl viologen).

5. 2. The screening method according to claim 1, wherein the amount of reactive oxygen species produced is measured using a chemiluminescent probe in the primary screening step.

6. 6. The screening method according to claim 5, wherein the chemiluminescent probe is MCLA (methyl cypridina luciferin analog).