Neural regenerating cells with alterations in DNA methylation
By altering the methylation state of specific genes in progenitor cells through transfection and culture, the method converts them into neural regenerating cells with improved regenerative capabilities, addressing the limitations of existing therapies.
USRE50635E1Active Publication Date: 2025-10-14SANBIO INC
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Patent Information
- Application Number
- US17/339167
- Authority / Receiving Office
- US · United States
- Patent Type
- Patents(United States)
- Current Assignee / Owner
- Priority Date
- 2008-04-30
- Filing Date
- 2021-06-04
- Publication Date
- 2025-10-14
- Estimated Expiration
- 2035-11-05
AI Technical Summary
Technical Problem
Existing methods for cellular therapy in neural disorders lack the ability to effectively stimulate neural recovery and regeneration after nervous system injury or disease.
Method used
Alter the methylation state of specific genes in progenitor cells, such as MASCs, by transfecting them with a polynucleotide encoding a Notch intracellular domain and culturing them to convert them into neural regenerating cells with altered methylation patterns.
Benefits of technology
The method effectively converts progenitor cells into neural regenerating cells with enhanced capabilities, as indicated by changes in methylation states of genes like PITX2, DNMT3b, IGF2R, and SDF4, demonstrating neural regenerative properties.
✦ Generated by Eureka AI based on patent content.
Abstract
Disclosed herein are cells, that are descendents of marrow adherent stem cells (MASCs), capable of rescuing and / or reversing various neural disorders after transplantation into sites of central nervous system (CNS) or peripheral nervous system (PNS) injury. The cells contain alterations in the methylation state of certain genes, compared to their methylation state in MASCs. Methods of making cells capable of rescuing and / or reversing various neural disorders after transplantation into sites of CNS or PNS injury, by alteration of the methylation status of certain genes, are also provided.
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