Devices and methods

WO2024188889A3PCT designated stage expired Publication Date: 2025-01-02CAMBRIDGE ENTERPRISE LTD +1
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Patent Information

Application Number
PCT/EP2024/056247
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-03-10
Filing Date
2024-03-08
Publication Date
2025-01-02

AI Technical Summary

Technical Problem

Conventional electroporation devices face challenges such as high voltage requirements, electric field distortion, heat generation, and metal ion dissolution, which negatively impact efficiency and cell viability, while previous attempts with PEDOT:PSS electrodes showed reduced electroporation efficiency compared to uncoated metal electrodes.

Method used

The use of PEDOT:PSS coated electrodes in a microfluidic device with interdigitated electrodes, optimized for dimensions and spacing to create a uniform electric field, and controlled voltage application for improved electroporation efficiency and cell viability, leveraging the reversible electrochemical properties of PEDOT:PSS to minimize Faradaic processes.

Benefits of technology

The PEDOT:PSS coated electrodes achieve improved electroporation efficiency and maintained cell viability across a wider range of operating conditions, significantly exceeding results with uncoated metal electrodes, with enhanced consistency and predictability in cell stimulation.

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Abstract

The present invention provides devices and methods for electroporation. Embodiments of the invention provide an electroporation device, the device having: a microfluidic channel with a fluid inlet and a fluid outlet; and a pair of interdigitated electrodes formed within said channel, wherein said electrodes are coated with PEDOT:PSS on a contact surface which is in contact with fluid in the channel. Other embodiments of the invention provide a method of performing electroporation on cells in a sample, the method including the steps of: introducing the sample into a chamber having a pair of electrodes each having a coating of PEDOT:PSS on a contact surface which is in contact with the sample; and applying oscillating stimulation voltage signals to the sample using the electrodes, wherein the stimulation voltage signals are applied so as to stimulate electroporation in the cells.
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Citation Information

Patent Citations

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  • Microfluidic Devices for Cells or Organ based Multimodal Activation and Monitoring system

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  • Method and Device for Exosomes Electroporation

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  • Electroporation Devices And Methods Of Cell Transfection

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