Nutrient composition for animals

Abstract

The purpose of the present invention is to provide a novel nutrient composition for animals. The present invention is a nutrient composition for a race horse, a nutrient composition for animals that suppresses a rise in the blood lactic acid concentration, and a nutrient composition for animals that improves coat. These compositions contain nicotinamide mononucleotide.

Description

Animal nutritional composition

[0001] The present invention relates to nutritional compositions for animals.

[0002] Nicotinamide mononucleotide is a coenzyme NAD ＋ Nicotinamide mononucleotide is a biosynthetic intermediate metabolite of nicotinamide mononucleotide. Non-Patent Document 1 reports that in a clinical trial of oral ingestion in humans, nicotinamide mononucleotide has the effects of improving obesity, preventing diabetes, and improving muscle insulin sensitivity. Furthermore, Non-Patent Document 2 reports that in a test using mice oral ingestion, nicotinamide mononucleotide has the effects of suppressing weight gain, improving insulin sensitivity, improving lipid metabolism, improving visual ability, increasing bone density, and increasing immune function. However, there have been no known cases of oral ingestion of nicotinamide mononucleotide in racehorses.

[0003] Mihoko Yoshino, Jun Yoshino, Brandon D Kayser, Gary J Patti, Michael P Franczyk, Kathryn F Mills, Miriam Sindelar, Terri Pietka, Bruce W Patterson, Shin-Ichiro Imai, Samuel Klein, Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women, Science 2021, 372, 1224-1229.Kathryn F. Mills, Shohei Yoshida, Liana R. Stein, Koji Uchida, Jun Yoshino, Shin-ichiro Imai, Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice Cell Metabolism 2016, 24, 795-806.

[0004] An object of the present invention is to provide a novel nutritional composition for animals.

[0005] The present invention includes the following: [1] A nutritional composition for a racehorse, comprising nicotinamide mononucleotide. [2] An animal nutritional composition for suppressing an increase in blood lactate concentration, comprising nicotinamide mononucleotide. [3] An animal nutritional composition for improving coat condition, comprising nicotinamide mononucleotide. [4] The animal nutritional composition according to [2] or [3], which is used for a racehorse.

[0006] The animal nutritional composition of the present invention can suppress an increase in blood lactate concentration and / or improve the coat condition of racehorses.

[0007] 1 is a diagram showing the measurement results of lactic acid concentration in Test Example 1. FIG. 2 is a diagram showing the average value of lactic acid concentration in Test Example 1.

[0008] [Nutritional Composition] The nutritional composition of the present invention (hereinafter also referred to as "the present nutritional composition") contains nicotinamide mononucleotide (hereinafter also referred to as "NMN").

[0009] <NMN> NMN (Chemical formula: C １１ H １５ N ２ O ８ P) exists as two optical isomers, α and β, but in the present invention, β-NMN (CAS number: 1094-61-7) is used. The structure of β-NMN is shown below.

[0010]

[0011] β-NMN may be prepared by any method. For example, purified β-NMN artificially synthesized by chemical synthesis, enzymatic methods, fermentation, or the like can be used. Furthermore, since β-NMN is a component widely present in living organisms, β-NMN obtained by extraction and purification from natural sources such as animals, plants, and microorganisms can also be used. Commercially available purified β-NMN may also be used.

[0012] As a chemical synthesis method for synthesizing β-NMN, for example, nicotinamide can be reacted with L-ribose tetraacetate and the resulting nicotinamide mononucleoside can be phosphorylated to produce β-NMN. As an enzymatic method, for example, β-NMN can be produced from nicotinamide and 5'-phosphoribosyl-1'-pyrophosphate (PRPP) using nicotinamide phosphoribosyltransferase (NAMPT). As a fermentation method, for example, β-NMN can be produced from nicotinamide using the metabolic system of a microorganism expressing NAMPT.

[0013] The NMN contained in the nutritional composition may be a pharmacologically acceptable salt of β-NMN. Pharmacologically acceptable salts of β-NMN may be inorganic acid salts or organic acid salts containing a basic moiety such as an amine. Examples of acids that constitute such acid salts include acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethenesulfonic acid, fumaric acid, gluconic acid, glutamic acid, hydrobromic acid, hydrochloric acid, isethionic acid, lactic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, mucic acid, nitric acid, pamoic acid, pantothenic acid, phosphoric acid, succinic acid, sulfuric acid, tartaric acid, and p-toluenesulfonic acid. Furthermore, pharmacologically acceptable salts of β-NMN may be alkali salts or organic salts containing an acidic moiety such as a carboxylic acid. Examples of bases that constitute such acid salts include alkali metal salts or alkaline earth metal salts, and include those derived from bases such as sodium hydride, potassium hydroxide, calcium hydroxide, aluminum hydroxide, lithium hydroxide, magnesium hydroxide, zinc hydroxide, ammonia, trimethylammonia, triethylammonia, ethylenediamine, lysine, arginine, ornithine, choline, N,N'-dibenzylethylenediamine, chloroprocaine, procaine, diethanolamine, N-benzylphenethylamine, diethylamine, piperazine, tris(hydroxymethyl)-aminomethane, and tetramethylammonium hydroxide.

[0014] The NMN contained in the nutritional composition may be free β-NMN or a solvate of a pharmacologically acceptable salt of β-NMN. Solvents that form such solvates include water, ethanol, etc.

[0015] The content of NMN contained in the nutritional composition of the present invention is not limited, but for example, when the total amount of the nutritional composition of the present invention is 100 parts by mass, the content may be 100 parts by mass, 1 to 99.9 parts by mass, or 1 to 50 parts by mass.

[0016] <Other Ingredients> The nutritional composition may contain ingredients other than NMN. For example, the nutritional composition may contain various vitamins (vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, etc.), various minerals, dietary fiber, polyunsaturated fatty acids, other nutrients (carotenoids such as β-carotene, lycopene, and astaxanthin, carnitine, and sesamin), stabilizers such as dispersants and emulsifiers, sweeteners, flavoring components (citric acid, malic acid, etc.), flavors, royal jelly, propolis, agaricus, etc. Herbs such as peppermint, bergamot, chamomile, and lavender may also be added.

[0017] <Dosage Form> Examples of the form of the animal nutritional composition of the present invention include powders, dustings, granules, soft capsules, hard capsules, tablets, chewable tablets, rapidly disintegrating tablets, syrups, liquids, suspensions, suppositories, ointments, creams, gels, adhesives, inhalants, and injections.

[0018] Examples of additives that can be used for formulation include animal oils such as soybean oil, saffron oil, olive oil, germ oil, sunflower oil, beef tallow, and sardine oil; polyhydric alcohols such as polyethylene glycol, propylene glycol, glycerin, and sorbitol; surfactants such as sorbitan fatty acid esters, sucrose fatty acid esters, glycerin fatty acid esters, and polyglycerin fatty acid esters; excipients such as purified water, lactose, starch, crystalline cellulose, D-mannitol, lecithin, gum arabic, sorbitol solution, and sugar solution; sweeteners, coloring agents, pH adjusters, and flavorings. Liquid formulations may be in a form that is dissolved or suspended in water or other suitable medium at the time of administration. Tablets and granules may be coated by well-known methods.

[0019] <Use> The nutritional composition can be ingested by animals as is or as an animal feed composition mixed with a basal animal feed. The nutritional composition can be ingested by animals throughout the entire rearing period or during a specific period of the rearing period.

[0020] A suitable example of an animal to which the nutritional composition is administered is a racehorse. By administering the composition to a racehorse, increases in blood lactate concentration can be suppressed, resulting in less muscle fatigue and less muscle pain, which can contribute to improving the performance of the racehorse. Racehorses include, for example, horses aged 2 to 7 years, as well as young horses currently in captivity. The dosage varies depending on the horse's age, weight, sex, etc., but is typically administered so that 1 g to 10 g of NMN is contained per administration. The daily dosage of NMN is, for example, 1 g to 30 g, preferably 1 g to 20 g, and more preferably 1 g to 15 g.

[0021] Animals other than racehorses to which the nutritional composition may be administered include animals other than humans, such as birds (e.g., chickens, quail, turkeys, ducks, etc.; e.g., poultry), mammals (bovine animals (e.g., domestic cattle (e.g., dairy cattle and / or beef cattle)), bison, buffalo), equine animals (e.g., horses, donkeys, zebras, etc.), sheep, goats, antelope, pigs, etc.). Examples of livestock include poultry, such as chickens (broilers, layers, etc.).

[0022] The nutritional composition can be used to suppress an increase in blood lactate concentration in an animal to which it is administered, or to improve coat condition. Improving coat condition means improving the coat's luster, quality, growth condition, color, etc.

[0023] [Animal feed composition] The animal feed composition of the present invention (hereinafter also referred to as "the present animal feed composition") contains NMN and a basal feed. The present animal feed composition may be in a form containing the above-mentioned present nutritional composition and a basal feed. Details of the components contained in the present nutritional composition are as described above.

[0024] The basal feed may be any commercially available basal feed, such as oil cakes, animal feed ingredients, grains and their processed products, animal fats and oils, vegetable fats and oils, or mixtures thereof. Oil cakes include dried foods such as corn, milo, and sorghum, soybean fats and oils, rapeseed fats and oils, sesame fats and oils, and cottonseed fats and oils. Animal feed ingredients include fish meal and meat-and-bone meal. Grains include starch, soybeans, wheat, and oats. Animal fats include meat meal, chicken meal, lard, and beef tallow. Vegetable fats include rapeseed oil, palm oil, coconut oil, soybean oil, safflower oil, corn oil, and cottonseed oil. The CP of basal feed refers to crude protein (one of the six components of feed, 6.25 times the nitrogen content), and basal feed such as CP 18% (crude protein content of 18%) or CP 19% can be used.

[0025] The basal feed may contain additives other than the nutritional composition. Examples of additives include seaweed. Seaweed contains many nutrients, such as minerals such as calcium, zinc, and iodine, dietary fiber such as sodium alginate, potassium alginate, and calcium alginate, polysaccharides such as fucoidan and funoran, and aminopeptides such as nori peptides and wakame peptides. Other additives include collagen, which may be in the form of an extract, dried product, pulverized product, or powder.

[0026] In addition to the above, examples of additives include fatty acids such as linoleic acid, linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid; minerals such as salt, calcium carbonate, calcium phosphate, and anhydrous silicic acid; vitamins such as vitamin A, vitamin B2, vitamin D, vitamin E, vitamin K, biotin, folic acid, and pantothenic acid; amino acids such as lysine, glycine, and methionine; emulsifiers such as sorbitan fatty acid esters, sucrose fatty acid esters, and lecithin; probiotics such as lactic acid bacteria; trace minerals such as iron, zinc, manganese, and copper; mushrooms or mushroom components such as sparassis crispa, maitake mushroom, shiitake mushroom, and cordyceps; sake lees; antioxidants; antifungal agents; and dietary fiber.

[0027] The content of NMN in the animal feed composition is not particularly limited, but can be, for example, 0.005 to 20 parts by mass, preferably 0.01 to 10 parts by mass, and more preferably 0.02 to 5 parts by mass, relative to 100 parts by mass of the basal feed. The dosage of the animal feed composition varies depending on the age, weight, sex, etc. of the animal, but for example, 1 kg to 8 kg can be administered per administration, and for example, 18 kg to 24 kg can be given per day.

[0028] The details of the target animals to be fed with the animal feed composition are as described above.

[0029] The present invention will be explained in more detail below with reference to examples, but the present invention is not limited to these examples.

[0030] [Preparation of nutritional composition] Nutritional composition A having the composition shown in Table 1 and basal feed A (containing oats, soybean meal (manufactured by Nippon Nosan Kogyo), and feed (trade name: Wizard, manufactured by Crossfield Co., Ltd.)) were prepared.

[0031]

[0032] [Test Example 1] In Test Example 1, four racehorses (racehorse A (7 years old), racehorse B (4 years old), racehorse C (8 years old), and racehorse D (3 years old)) were fed as follows, and their blood lactate concentrations and coat condition were evaluated.

[0033] <Evaluation of Blood Lactate Concentration> Each racehorse was administered basal feed A for 14 consecutive days. The feeding amount was 6 to 8 kg three times a day. On the 14th day, after 80 to 100 minutes of regular training (60 minutes on a treadmill, 20 to 40 minutes of training), blood was collected from the neck vein and the blood lactate concentration was measured using a lactate meter (product name: Lactate Pro 2LT-1730, manufactured by Arkray, Inc.) (hereinafter also referred to as the "pre-intake test"). Thereafter, each racehorse was fed basal feed A and nutritional composition A for 14 consecutive days. The feeding amounts were 6 to 8 kg of basal feed A and 12 g of nutritional composition A three times a day, so that the daily feeding amount of NMN was 5.0 to 5.5 g. After 80 to 100 minutes of regular training, the blood lactate concentration was measured using the same method as above (hereinafter also referred to as the "post-intake test"). Figure 1 shows the measurement results of the lactate concentration of the four horses, and Figure 2 shows the average lactate concentration of the four horses.

[0034] <Evaluation of Coat Condition> The coat condition of each racehorse was visually compared between the pre-intake test and the post-intake test, and the coat condition of the post-intake test was evaluated according to the following criteria. The evaluation results are shown in Table 2. 3: Good improvement in gloss and texture was observed. 2: Slight improvement in gloss and texture was observed. 1: No improvement in coat condition was observed.

[0035]

[0036] 1 and 2, it was found that nutritional composition A has the effect of suppressing increases in blood lactate levels in racehorses. Furthermore, it was found from the results shown in Table 2 that nutritional composition A has the effect of improving the coat condition of racehorses.

Claims

1. A nutritional composition for racehorses comprising nicotinamide mononucleotide.

2. A nutritional composition for animals for suppressing an increase in blood lactate concentration, comprising nicotinamide mononucleotide.

3. An animal nutritional composition for improving coat condition, comprising nicotinamide mononucleotide.

4. The animal nutritional composition according to claim 2 or 3, which is used for racehorses.

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