Detection device and method for measuring anaerobic ammonium oxidation activity

Abstract

The present invention relates to the technical field of wastewater treatment, and in particular to a detection device and method for measuring anaerobic ammonium oxidation activity. The detection device comprises a reactor and a detector; the reactor comprises a first reaction chamber and a second reaction chamber; the first reaction chamber is used for an anaerobic ammonium oxidation reaction and a denitrification reaction of sludge to be tested; the second reaction chamber is used for a denitrification reaction of said sludge; and the detector is used for controlling and recording the flow rates of chemicals entering the first reaction chamber and the second reaction chamber, ensuring that the pH values of the first reaction chamber and the second reaction chamber are constant and the same. According to the device, the real-time activity and maximum activity of anaerobic ammonium oxidation can be obtained on the basis of the hydrogen ion consumption rate in the reaction process, avoiding the cumbersome process of continuously sampling and measuring the concentration of ammonia nitrogen or nitrite in a conventional method, and simplifying the operation process; the first reaction chamber and the second reaction chamber of the detection device use the same sludge as a control, and the interference caused by the denitrification process using endogenous organic matter as an electron donor is eliminated, improving the measurement precision.

Description

Detection device and method for measuring anaerobic ammonium oxidation activity

[0001] This application claims priority to a Chinese patent application filed with the Patent Office of China on July 19, 2023, with application number 202310899960.5 and application name “A detection device and method for determining anaerobic ammonia oxidation activity”, the entire contents of which are incorporated herein by reference. Technical Field

[0002] The present invention belongs to the technical field of sewage treatment, and in particular relates to a detection device and method for measuring anaerobic ammonium oxidation activity. Background Art

[0003] Anaerobic ammonium oxidation (ANAMMOX) is a biological reaction using ammonia as an electron donor and nitrite as an electron acceptor. First discovered in fluidized bed reactors, it is widely recognized as one of the most cost-effective biological denitrification processes due to its advantages, including the lack of an external organic carbon source, high denitrification efficiency, low operating costs, and minimal footprint. Within the wastewater treatment process, various pathways, such as traditional nitrification-denitrification and sulfur autotrophic denitrification, play distinct roles in the denitrification process. Therefore, distinguishing these pathways, particularly the contribution of the ANAMMOX pathway, is a pressing issue in ANAMMOX process regulation and engineering applications.

[0004] Some existing technologies use isotope-labeled NO2 - Some methods use nitrogen as a substrate and measure the production rate of labeled nitrogen during anaerobic ammonium oxidation to calculate the anaerobic ammonium oxidation rate. Some methods calculate the total nitrogen removal rate by measuring the changes in the concentration of various nitrogen-containing components in the sludge during denitrification and anaerobic ammonium oxidation, and then calculate the anaerobic ammonium oxidation rate by subtracting the denitrification process. Existing technologies require the use of isotope tracing technology or the collection of multiple water samples to measure multiple water quality indicators, which is labor-intensive, costly, and requires complex equipment and instruments.

[0005] Summary of the Invention

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the problem that the existing technology mostly uses NO2 - The anaerobic ammonium oxidation rate is tested as a substrate. This technology has the following defects: complex equipment, high monitoring cost, large workload, many equipment and instruments, and large human errors. Therefore, a detection device and method for measuring anaerobic ammonium oxidation activity are provided.

[0007] To this end, the present invention provides the following technical solutions.

[0008] The present invention provides a detection device for measuring anaerobic ammonium oxidation activity, comprising a reactor and a detector; wherein,

[0009] The reactor performs anaerobic ammonium oxidation reaction and denitrification reaction, including:

[0010] The first reaction chamber is used for anaerobic ammonium oxidation and denitrification of the sludge to be tested;

[0011] The second reaction chamber is used for denitrification reaction of the sludge to be tested;

[0012] The detector is used to control and record the flow rate of the reagent entering the first reaction chamber and the second reaction chamber, ensuring that the pH values ​​of the first reaction chamber and the second reaction chamber are constant and the same.

[0013] The first reaction chamber and the second reaction chamber are connected or isolated by a partition;

[0014] Preferably, the first reaction chamber and the second reaction chamber have the same volume.

[0015] The detection device satisfies at least one of (1)-(2):

[0016] (1) A first sealing cover plate is provided on the top of the first reaction chamber;

[0017] Preferably, a first pH probe is provided on the sealing cover plate, and the detector monitors the pH value of the first reaction chamber through the first pH probe;

[0018] (2) A second sealing cover plate is provided on the top of the second reaction chamber;

[0019] Preferably, a second pH probe is provided on the sealing cover, and the detector monitors the pH value of the second reaction chamber through the second pH probe.

[0020] The detection device satisfies at least one of (1) to (3):

[0021] (1) The first reaction chamber is further provided with a first gas dispersion unit for delivering gas to the first reaction chamber; and / or,

[0022] A first medicine supply port, used for supplying medicine;

[0023] a first inlet for conveying materials and / or exhausting gas;

[0024] (2) The second reaction chamber is further provided with a second gas dispersion unit for delivering gas to the second reaction chamber; and / or,

[0025] The second medicine supply port is used to supply medicine;

[0026] a second inlet for conveying materials and / or exhausting gas;

[0027] (3) The detection device is also provided with a constant temperature heater to ensure that the temperatures of the first reaction chamber and the second reaction chamber are the same and constant.

[0028] The detector is further provided with a controller having a built-in PID control algorithm for controlling and recording the flow of the reagent entering the first reaction chamber and the second reaction chamber; and / or,

[0029] The detector is also provided with a medicine storage tank.

[0030] The present invention also provides a method for determining anaerobic ammonium oxidation activity, comprising:

[0031] (1) the sludge to be tested undergoes an anaerobic ammonium oxidation reaction and a denitrification reaction in a first reaction chamber; the sludge to be tested undergoes a denitrification reaction in a second reaction chamber; wherein the rates of the denitrification reaction in the first reaction chamber and the second reaction chamber are the same;

[0032] (2) During the reaction process, the flow rate of the reagent entering the first reaction chamber and the second reaction chamber is controlled and recorded by the detector to ensure that the pH of the first reaction chamber and the second reaction chamber are constant and the same; wherein, the rate of the denitrification reaction in the first reaction chamber and the second reaction chamber is ensured to be the same by controlling the temperature and pH of the first reaction chamber and the second reaction chamber to be constant and the same;

[0033] (3) The real-time consumption rate of hydrogen ions is calculated based on the concentration of the reagent added to the first reaction chamber and the second reaction chamber, the reagent flow rate in the first reaction chamber, and the reagent flow rate in the second reaction chamber. The real-time ammonia nitrogen conversion rate of the anaerobic ammonium oxidation process, i.e., the real-time activity of anaerobic ammonium oxidation, is obtained based on the corresponding relationship between the hydrogen ion consumption rate and the ammonia nitrogen consumption rate.

[0034] The detection method further comprises: obtaining the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen based on the real-time activity of anaerobic ammonium oxidation;

[0035] Preferably, the method further comprises: obtaining the maximum specific activity of anaerobic ammonium oxidation activity according to the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen and the sludge concentration.

[0036] Furthermore, the real-time ammonia nitrogen conversion rate during the anaerobic ammonium oxidation process is obtained by formula I;

[0037] Among them, dC NH / dt is the real-time ammonia nitrogen conversion rate, unit: mgN·L -1 min -1 ;

[0038] t is time, unit: min;

[0039] C His the hydrogen ion concentration in the total reagent supply of the first reaction chamber and the second reaction chamber, unit: mmol / L, that is, the real-time consumption rate of hydrogen ions; the total reagent supply of the first reaction chamber and the second reaction chamber is obtained by calculating the reagent flow rate and its concentration entering the first reaction chamber and the second reaction chamber.

[0040] Q A is the flow rate of the reagent added to chamber A, unit: L / min;

[0041] Q B is the flow rate of the reagent added to chamber B, unit: L / min;

[0042] 0.13 is the hydrogen ion corresponding to 1 mol of ammonia nitrogen consumed in the anaerobic ammonium oxidation reaction;

[0043] V is the volume of the liquid phase in the first reaction chamber or the second reaction chamber, in L.

[0044] Furthermore, according to formula II, the maximum specific activity μ of anaerobic ammonium oxidation is obtained:

[0045] Where v is the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen, unit: mgN·L -1 min -1 ;

[0046] MLVSS refers to the concentration of sludge in the first reaction chamber after the reaction is completed, unit: mgMLVSS·L -1 ;

[0047] μ, unit: mgN·min -1 mgMLVSS -1 .

[0048] The detection method satisfies at least one of (1) to (6):

[0049] (1) When the anaerobic ammonium oxidation and / or denitrification reaction is carried out, the supplied agent is a H + of solution;

[0050] (2) Using PID to control the amount of reagent supply to ensure constant pH in the first and second reaction chambers;

[0051] (3) Obtaining the maximum rate v of anaerobic ammonium oxidation degradation of ammonia nitrogen based on the real-time ammonia nitrogen conversion rate versus time curve of the anaerobic ammonium oxidation process;

[0052] (4) adding nitrite and ammonia nitrogen into the first reaction chamber to carry out anaerobic ammonium oxidation reaction and denitrification reaction;

[0053] Preferably, the concentration of nitrite in the first reaction chamber is not higher than 50 mg N / L;

[0054] Preferably, the concentration of ammonia nitrogen in the first reaction chamber is not higher than 50 mg N / L;

[0055] (5) adding nitrite into the second reaction chamber to carry out denitrification reaction;

[0056] Preferably, the concentration of nitrite in the second reaction chamber is not higher than 50 mg N / L;

[0057] (6) The concentration of the sludge to be tested is 4000 mg / L-6000 mg / L.

[0058] The technical solution of the present invention has the following advantages:

[0059] 1. The present invention provides a detection device for measuring anaerobic ammonium oxidation activity, which includes a reactor and a detector; wherein the reactor performs anaerobic ammonium oxidation reaction and denitrification reaction, including a first reaction chamber and a second reaction chamber, the first reaction chamber is used for the sludge to be tested to perform anaerobic ammonium oxidation reaction and denitrification reaction; the second reaction chamber is used for the sludge to be tested to perform denitrification reaction; the detector is used to control and record the flow rate of the reagent entering the first reaction chamber and the second reaction chamber, ensuring that the pH value of the first reaction chamber and the second reaction chamber is constant and the same. When the device is used to measure anaerobic ammonium oxidation activity, the real-time activity and maximum activity of anaerobic ammonium oxidation can be obtained by the real-time consumption rate of hydrogen ions during the reaction process, avoiding the cumbersome process of continuous sampling and measuring ammonia nitrogen or nitrite concentration of the traditional method, simplifying the operation process, reducing human error, the detection device system can be integrated, the measurement process does not require a spectrophotometer, water quality analyzer and other instruments, and is easy to carry; the first reaction chamber and the second reaction chamber of the detection device use the same sludge as a control, deducting the interference caused by the denitrification process with endogenous organic matter as an electron donor, thereby improving the measurement accuracy.

[0060] 2. The present invention provides a detection device for measuring anaerobic ammonia oxidation activity. The detector in the detection device obtains the pH value in the reaction chamber through a pH probe and controls the amount of reagent supply, which simplifies the equipment, reduces costs, and increases the frequency of data acquisition.

[0061] 3. The present invention provides a detection method for determining anaerobic ammonium oxidation activity. When determining anaerobic ammonium oxidation activity, the detection method can obtain the real-time activity and maximum activity of anaerobic ammonium oxidation by the hydrogen ion consumption rate during the reaction process, replacing the traditional method of determining anaerobic ammonium oxidation activity using ammonia nitrogen, nitrite, etc. as substrates. Compared with traditional technologies, the detection method of the present invention reduces measurement time and workload, simplifies equipment, and when detecting anaerobic ammonium oxidation activity, the first reaction chamber and the second reaction chamber react together, deducting the measurement error caused by the denitrification process with endogenous organic matter as electron donors, thereby improving measurement accuracy; at the same time, the pH value is maintained constant, ensuring that H + Accuracy of consumption measurement.

[0062] The detection method of the present invention adopts PID to control the dosage, maintains the pH constant, and further ensures the H + Accuracy of consumption measurement. BRIEF DESCRIPTION OF THE DRAWINGS

[0063] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0064] FIG1 is a detection device for measuring anaerobic ammonium oxidation activity in Example 1 of the present invention;

[0065] FIG2 is a graph showing the real-time ammonia nitrogen conversion rate during the anaerobic ammonium oxidation process in Example 2 of the present invention;

[0066] Figure markings: 1-first reaction chamber; 2-second reaction chamber; 3-gas storage tank; 4-first pH probe; 5-second pH probe; 6-controller; 7-chemical storage tank; 8-second dosing pump; 9-first dosing pump; 10-partition; 1-1-first gas dispersion unit; 1-2-first agitator; 1-3-first inlet; 1-4-first chemical supply port; 1-5-first sealing cover plate; 2-1-second gas dispersion unit; 2-2-second agitator; 2-3-second inlet; 2-4-second chemical supply port; 2-5-second sealing cover plate. DETAILED DESCRIPTION

[0067] The following examples are provided for a better understanding of the present invention and are not intended to limit the best mode of implementation. They do not limit the content and scope of protection of the present invention. Any product identical or similar to the present invention obtained by anyone under the guidance of the present invention or by combining the features of the present invention with other prior arts shall fall within the scope of protection of the present invention.

[0068] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in this field can be used. If the manufacturer of the reagents or instruments is not specified, they are all commercially available conventional reagents.

[0069] Example 1

[0070] This embodiment provides a detection device for measuring anaerobic ammonium oxidation activity, as shown in FIG1 , comprising a reactor and a detector; wherein:

[0071] The reactor is used for performing anaerobic ammonium oxidation reaction and denitrification reaction; the reactor includes a first reaction chamber 1 and a second reaction chamber 2, the first reaction chamber 1 is used for performing anaerobic ammonium oxidation reaction and denitrification reaction on the sludge to be tested, and the second reaction chamber 2 is used for performing denitrification reaction on the sludge to be tested; the reactor also includes a partition 10, which can be moved to achieve communication or isolation between the first reaction chamber 1 and the second reaction chamber 2.

[0072] A first sealing cover plate 1-5 is provided on the top of the first reaction chamber 1 for sealing the first reaction chamber 1; a first gas dispersion unit 1-1 is provided at the bottom of the first reaction chamber 1 for transporting the gas in the gas storage tank 3 into the first reaction chamber 1, and a first inlet 1-3 and a first reagent supply port 1-4 are provided on the first sealing cover plate of the first reaction chamber 1. The first inlet 1-3 is used to discharge the gas in the first reaction chamber 1 and / or transport materials into the first reaction chamber, and the first reagent supply port 1-4 is used to replenish the reagent.

[0073] A second sealing cover plate 2-5 is provided on the top of the second reaction chamber 2 for sealing the second reaction chamber 2; a second gas dispersion unit 2-1 is provided at the bottom of the second reaction chamber 2 for transporting the gas in the gas storage tank 3 into the second reaction chamber 2, and a second inlet 2-3 and a second agent supply port 2-4 are provided on the second sealing cover plate of the second reaction chamber 2. The second inlet 2-3 is used to discharge the gas in the second reaction chamber 2 and / or transport materials into the second reaction chamber, and the second agent supply port 2-4 is used to replenish the agent.

[0074] The detector is used to control and record the flow rate of the reagent entering the first reaction chamber 1 and the second reaction chamber 2, ensuring that the pH values ​​of the first reaction chamber 1 and the second reaction chamber 2 are constant and identical. The detector includes a first pH probe 4 and a second pH probe 5. The detector monitors the pH value of the first reaction chamber 1 via the first pH probe 4, and the detector monitors the pH value of the second reaction chamber 2 via the second pH probe 5. The detector also includes a controller 6, a first dosing pump 9, a second dosing pump 8, and a reagent storage tank 7. The controller 6 has a built-in PID algorithm for controlling and recording the flow rate of the reagent entering the first and second reaction chambers. The reagent is replenished to the first reaction chamber 1 via the first dosing pump 9 and to the second reaction chamber 2 via the second dosing pump 8. The reagent to be replenished is stored in the reagent storage tank 7.

[0075] Furthermore, the temperatures of the first and second reaction chambers must be maintained constant and identical. Preferably, the first and second reaction chambers can be placed in a constant-temperature heating device, such as a water bath, to ensure that the temperatures of the two reaction chambers are constant and identical. Furthermore, the temperatures of the first and second reaction chambers can be measured using a temperature sensor, such as a thermometer in the constant-temperature heating device or a temperature sensor built into a detector. The above description is not specifically limited.

[0076] Furthermore, a first stirrer 1-2 is provided at the bottom of the first reaction chamber 1, and a second stirrer 2-2 is provided at the bottom of the second reaction chamber 2, for stirring the materials in the reaction chambers. The stirrer can be, but is not limited to, a stirring rotor.

[0077] In a specific embodiment, the sludge to be tested, ammonia nitrogen and nitrite undergo anaerobic ammonium oxidation reaction and denitrification reaction in the first reaction chamber 1; the sludge to be tested and nitrite undergo denitrification reaction in the second reaction chamber; wherein, ammonia nitrogen can be but not limited to ammonium sulfate and ammonium hydrochloride; nitrite can be but not limited to potassium nitrite and sodium nitrite; the concentration of the sludge to be tested is not specifically limited and can be any concentration between 4000-6000 mg / L, and the source of the sludge to be tested is an anaerobic ammonium oxidation reaction system under artificial or natural conditions; there is no requirement for the ratio of the sludge to be tested and nitrite and ammonia nitrogen.

[0078] Preferably, the volumes of the first reaction chamber and the second reaction chamber are the same; further, the amounts of the sludge to be tested and the nitrite in the first reaction chamber and the second reaction chamber are the same to ensure the same denitrification reaction rate.

[0079] Furthermore, in a specific embodiment, the reagent storage tank 7 contains a solution containing hydrogen ions, such as but not limited to a hydrochloric acid solution, a sulfuric acid solution, etc. The gas in the gas storage tank 3 can be but not limited to nitrogen.

[0080] Example 2

[0081] This embodiment provides a method for determining anaerobic ammonium oxidation activity, using the detection device provided in Example 1, comprising the following steps:

[0082] (1) Take an appropriate amount of anaerobic ammonium oxidation sludge as the sludge to be tested, wash it with deionized water, and starve it for 2 hours; put the sludge mixture to be tested into the reactor and dilute it proportionally to the sludge concentration in the reactor to 4000-6000 mg / L, seal the first reaction chamber and the second reaction chamber, keep the partition raised, so that the first reaction chamber and the second reaction chamber are connected, turn on the agitator 1-2 and the agitator 2-2, and pass the nitrogen in the gas storage tank 3 into the first reaction chamber 1 and the second reaction chamber 2 through the first gas dispersion unit 1-1 and the second gas dispersion unit 2-1. After the dissolved oxygen in the first reaction chamber and the second reaction chamber is emptied, stop passing nitrogen, and the dissolved oxygen is discharged through the first inlet 1-3 and the second inlet 2-3; after the dissolved oxygen is emptied, press the partition to isolate the first reaction chamber and the second reaction chamber. In this embodiment, the concentration of the sludge to be tested in the first reaction chamber and the second reaction chamber after dilution with water is 4200 mg / L, and the sludge to be tested comes from the anaerobic ammonium oxidation reaction system under artificial or natural conditions.

[0083] Nitrite and ammonia nitrogen are added to the first reaction chamber 1 through the first inlet 1-3, and anaerobic ammonium oxidation reaction and denitrification reaction are carried out simultaneously in the first reaction chamber; nitrite is added to the second reaction chamber 2 through the second inlet 2-3, ensuring that the volume and concentration of nitrite added to the first reaction chamber and the second reaction chamber are the same, maintaining the rotation of the agitator, ensuring that the temperature and pH of the two chambers are the same, and the denitrification reaction rate is the same. In this embodiment, the nitrite in each reaction chamber is a NaNO2 aqueous solution with a concentration of 400mgN / L and an addition amount of 100ml, and the ammonia nitrogen in the first reaction chamber is a (NH4)2SO4 aqueous solution with a concentration of 400mgN / L and an addition amount of 100ml; after the addition of the agent, the volume of the solutions in the two chambers is constant to 1L. The mass ratio of nitrite to the sludge to be tested is not specifically limited. The first reaction chamber and the second reaction chamber are placed in a water bath heating device, the water bath temperature is controlled at 30-35°C, and the temperature is kept constant. Constant and identical temperature and pH can ensure that the denitrification reaction rate in the two reaction chambers is the same.

[0084] (2) The reaction is started. During the reaction, the flow rate of the reagent entering the first reaction chamber and the second reaction chamber is controlled and recorded by the detector to ensure that the pH value of the two chambers is constant and the same during the reaction. The detector has a built-in PID algorithm. Under PID control, the speed of the dosing pump is adjusted according to the pH value of each chamber and the system preset pH target value of 7.5 to control the pH of the two chambers at a constant level. The supplied reagent is added to the two reaction chambers through the first reagent supply port 1-4 and the second reagent supply port 2-4. The PID parameters can be tuned using the Cohen-Coon parameter tuning method or the Ziegler-Nichols parameter tuning method. The control composition is shown in the following formula:

[0085] The P (proportional) part of this control represents the difference between the control parameter and the target value, the I (integral) part represents the accumulated error, and the D (differential) part represents the prediction of future differences.

[0086] (3) According to the following stoichiometric equation of the anaerobic ammonium oxidation reaction process, the hydrogen ion corresponding to 1 mol of ammonia nitrogen consumed in the anaerobic ammonium oxidation reaction can be obtained, that is, for every 1 mol of ammonia nitrogen consumed, 0.13 mol of H + ; 1NH4 + +1.32NO2 - +0.066HCO3 - +0.13H + →1.02N2+0.26NO3 - +0.066CH2O 0.5 N 0. 15 +2.03H2O

[0087] The real-time consumption rate of hydrogen ions is calculated based on the concentration of the reagent added to the first reaction chamber and the second reaction chamber, the flow rate of the reagent in the first reaction chamber, and the flow rate of the reagent in the second reaction chamber. The real-time ammonia nitrogen conversion rate of the anaerobic ammonium oxidation process, i.e., the real-time activity of anaerobic ammonium oxidation, is obtained based on the corresponding relationship between the hydrogen ion consumption rate and the ammonia nitrogen consumption rate. That is, the real-time ammonia nitrogen conversion rate of the anaerobic ammonium oxidation process is obtained according to formula I.

[0088] Among them, dC NH / dt is the real-time ammonia nitrogen conversion rate, unit: mgN·L -1 min -1 ;

[0089] t is time, unit: min;

[0090] C His the hydrogen ion concentration in the total reagent supply to the first reaction chamber and the second reaction chamber, that is, the real-time consumption rate of hydrogen ions, in mmol / L; wherein the total reagent supply to the first reaction chamber and the second reaction chamber is calculated based on the reagent flow rate and reagent concentration entering the first reaction chamber and the second reaction chamber;

[0091] Q A is the flow rate of the reagent added to chamber A, unit: L / min;

[0092] Q B is the flow rate of the reagent added to chamber B, unit: L / min;

[0093] 0.13 is the hydrogen ion corresponding to 1 mol of ammonia nitrogen consumed in the anaerobic ammonium oxidation reaction;

[0094] V is the volume of the liquid phase in the first reaction chamber or the second reaction chamber, in L. In this embodiment, the real-time ammonia nitrogen conversion rate during the anaerobic ammonium oxidation process is shown in FIG2 , that is, the real-time anaerobic ammonium oxidation activity.

[0095] (4) Furthermore, the maximum rate of anaerobic ammonia oxidation degradation of ammonia nitrogen v (i.e., dNH / dt max ), the unit is mgN·L -1 min -1 .

[0096] Furthermore, according to formula II, the maximum specific activity μ of anaerobic ammonium oxidation is obtained.

[0097] Wherein, v is the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen, unit: mgN / L / min;

[0098] MLVSS refers to the concentration of sludge in the first reaction chamber after the reaction is completed, and the unit is mgMLVSS·L -1 ;

[0099] μ, unit: mgN·min -1 mgMLVSS -1 In this embodiment, the concentration of sludge in the first reaction chamber after the reaction is 4200 mg MLVSS·L -1 The maximum degradation rate of ammonia nitrogen by anaerobic ammonium oxidation is 8.9×10 -2 mgN·L -1 min -1 The maximum specific activity μ of anaerobic ammonium oxidation was 2.12×10 -5 mgN·min -1 mgMLVSS -1 .

[0100] The detection method of this embodiment can obtain the anaerobic ammonium oxidation activity by the hydrogen ion consumption rate during the reaction process when measuring the anaerobic ammonium oxidation activity, replacing the method of measuring the anaerobic ammonium oxidation activity using ammonia nitrogen, nitrite, etc. as substrates in traditional technologies. Compared with traditional technologies, the detection method of the present invention reduces measurement time and workload, simplifies equipment, and when measuring the anaerobic ammonium oxidation activity, the first reaction chamber and the second reaction chamber react together, thereby eliminating the measurement error caused by the denitrification process using endogenous organic matter as an electron donor, thereby improving the measurement accuracy.

[0101] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A detection device for measuring anaerobic ammonium oxidation activity, characterized in that: comprising a reactor and a detector; wherein, The reactor performs anaerobic ammonium oxidation reaction and denitrification reaction, including: The first reaction chamber is used for anaerobic ammonium oxidation and denitrification of the sludge to be tested; The second reaction chamber is used for denitrification of the sludge to be tested; The detector is used to control and record the flow rate of the reagent entering the first reaction chamber and the second reaction chamber, so as to ensure that the pH values ​​of the first reaction chamber and the second reaction chamber are constant and the same.

2. The detection device according to claim 1, characterized in that: The first reaction chamber and the second reaction chamber are connected or isolated by a partition; Preferably, the first reaction chamber and the second reaction chamber have the same volume.

3. The detection device according to claim 1 or 2, characterized in that: Satisfy at least one of (1)-(2): (1) A first sealing cover plate is provided on the top of the first reaction chamber; Preferably, a first pH probe is provided on the sealing cover plate, and the detector monitors the pH value of the first reaction chamber through the first pH probe; (2) A second sealing cover plate is provided on the top of the second reaction chamber; Preferably, a second pH probe is provided on the sealing cover plate, and the detector monitors the pH value of the second reaction chamber through the second pH probe.

4. The detection device according to any one of claims 1 to 3, characterized in that: Satisfy at least one of (1)-(3): (1) The first reaction chamber is further provided with a first gas dispersion unit for delivering gas to the first reaction chamber; and / or, A first medicine supply port, used for supplying medicine; A first inlet for conveying materials and / or exhausting gas; (2) the second reaction chamber is further provided with a second gas dispersion unit for delivering gas to the second reaction chamber; and / or, The second medicine supply port is used to supply medicine; A second inlet for conveying materials and / or exhausting gas; (3) The detection device is also provided with a constant temperature heater to ensure that the temperatures of the first reaction chamber and the second reaction chamber are the same and constant.

5. The detection device according to any one of claims 1 to 4, characterized in that: The detector is further provided with a controller, wherein the controller has a built-in PID control algorithm for controlling and recording the flow of the drug entering the first reaction chamber and the second reaction chamber; and / or, The detector is also provided with a medicine storage tank.

6. A method for determining anaerobic ammonium oxidation activity, characterized in that: include: (1) the sludge to be tested is subjected to anaerobic ammonium oxidation reaction and denitrification reaction in the first reaction chamber; the sludge to be tested is subjected to denitrification reaction in the second reaction chamber; wherein the rate of the denitrification reaction in the first reaction chamber and the second reaction chamber is the same; (2) During the reaction process, the flow rate of the reagent entering the first reaction chamber and the second reaction chamber is controlled and recorded by the detector to ensure that the pH of the first reaction chamber and the second reaction chamber is constant and the same; (3) The real-time consumption rate of hydrogen ions is calculated by combining the concentration of the reagent added to the first reaction chamber and the second reaction chamber, the reagent flow rate in the first reaction chamber, and the reagent flow rate in the second reaction chamber. The real-time ammonia nitrogen conversion rate of the anaerobic ammonium oxidation process, i.e., the real-time activity of anaerobic ammonium oxidation, is obtained based on the corresponding relationship between the hydrogen ion consumption rate and the ammonia nitrogen consumption rate.

7. The detection method according to claim 6, characterized in that: Also includes: The maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen was obtained based on the real-time activity of anaerobic ammonium oxidation; Preferably, the method further comprises: obtaining the maximum specific activity of anaerobic ammonium oxidation activity according to the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen and the sludge concentration.

8. The detection method according to claim 6, characterized in that: The real-time ammonia nitrogen conversion rate during anaerobic ammonium oxidation is obtained by formula I; Among them, dC NH / dt is the real-time ammonia nitrogen conversion rate, unit: mgN·L -1 ·min -1 ; t is time, unit: min; C H is the hydrogen ion concentration in the total reagent supply of the first reaction chamber and the second reaction chamber, unit: mmol / L; Q A is the flow rate of the reagent added to chamber A, unit: L / min; Q B is the flow rate of the reagent added to chamber B, unit: L / min; 0.13 is the hydrogen ion corresponding to 1 mol of ammonia nitrogen consumed in the anaerobic ammonium oxidation reaction; V is the volume of the liquid phase in the first reaction chamber or the second reaction chamber, in L.

9. The detection method according to claim 7, characterized in that: According to formula II, the maximum specific activity μ of anaerobic ammonium oxidation is obtained: Where v is the maximum rate of anaerobic ammonium oxidation degradation of ammonia nitrogen, unit: mgN·L -1 ·min -1 ; MLVSS refers to the concentration of sludge in the first reaction chamber after the reaction is completed, unit: mgMLVSS·L -1 ; μ, unit: mgN·min -1 mgMLVSS -1 .

10. The detection method according to any one of claims 6 to 9, characterized in that: Satisfy at least one of (1)-(6): (1) When performing the anaerobic ammonium oxidation and / or denitrification reaction, the reagent supplied is a H-containing + of solution; (2) Using PID to control the amount of reagent supply to ensure that the pH of the first reaction chamber and the second reaction chamber is constant; (3) obtaining the maximum rate v of anaerobic ammonium oxidation degradation of ammonia nitrogen based on the real-time ammonia nitrogen conversion rate versus time curve of the anaerobic ammonium oxidation process; (4) adding nitrite and ammonia nitrogen into the first reaction chamber to carry out anaerobic ammonium oxidation reaction and denitrification reaction; Preferably, the concentration of nitrite in the first reaction chamber is not higher than 50 mg N / L; Preferably, the concentration of ammonia nitrogen in the first reaction chamber is not higher than 50 mg N / L; (5) adding nitrite into the second reaction chamber to carry out a denitrification reaction; Preferably, the concentration of nitrite in the second reaction chamber is not higher than 50 mg N / L; (6) The concentration of the sludge to be tested is 4000 mg / L-6000 mg / L.

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