Antioxidant inner beauty health food composition, and processed food and health functional food comprising same

Abstract

The present invention relates to an antioxidant inner beauty health food composition, and a processed food and a health functional food comprising same and, more specifically, to an antioxidant inner beauty health food composition, and a processed food and a health functional food comprising same, wherein a Haematococcus pluvialis processed product, an aloe vera processed product, a red grape processed product, a ginger processed product, and a star anise processed product, which are materials derived from natural materials and are harmless to the body and has excellent antioxidant efficacy, are contained as active ingredients.

Description

Antioxidant inner beauty health food composition, processed food and health functional food containing the same

[0001] The present invention relates to an antioxidant inner beauty health food composition having a skin defense effect through antioxidant activity by including, as active ingredients, a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, and a processed product of star anise, which are known to have antioxidant activity, and a processed food and a health functional food comprising the same.

[0002] The skin plays a vital role in protecting the body, regulating and conserving moisture and temperature, and maintaining homeostasis. Skin aging occurs as a result of complex biological processes, including a decline in the regenerative capacity of skin cells. It can be categorized into intrinsic aging, which occurs naturally over time, and extrinsic aging, which occurs due to exposure to UV rays.

[0003] Intrinsic aging is known to be caused by factors such as immune suppression, epidermal aging imbalances, hormonal changes, aging, stress, or an individual's genetic makeup. This intrinsic aging is characterized by decreased elasticity due to a decrease in cell number, fine wrinkles due to structural changes in the stratum corneum caused by moisture loss, and a decrease in the subcutaneous fat layer. Its impact on the skin is relatively minor.

[0004] On the other hand, extrinsic aging is known to be caused by oxidative stress due to UV rays or external substances. In particular, reactive oxygen species (ROS) generated by UV rays activate various transcription factors, increasing the production of cytokines related to inflammation and enzymes that degrade matrix proteins such as collagen and elastin in the dermis, or inhibiting enzymes that synthesize them. These reactive oxygen species reduce matrix proteins that maintain skin tension, strength, and elasticity, and play a role in protecting the skin, resulting in coarse and deep wrinkles, dry skin, and decreased elasticity. More than 80% of this extrinsic aging is caused by photoaging, which has detrimental effects on the skin, including wrinkle formation, dry skin, pigmentation, and peripheral vasodilation, caused by medium-wave ultraviolet B (UVB) rays.

[0005] In this regard, much research and development is being conducted on antioxidants that inhibit oxidation caused by free radicals. Antioxidants are widely distributed throughout the animal and plant kingdoms, and many physiologically active ingredients, such as tocopherol, vitamin C, and selenium, are known to be found in fruits and vegetables. By inhibiting oxidation caused by free radicals, these antioxidants can protect the skin by preventing damage to matrix proteins that play a protective role in the skin.

[0006] However, naturally occurring antioxidants are not expected to be sufficiently effective when applied to the skin. Therefore, synthetic antioxidants, which offer superior antioxidant properties and are inexpensive, are widely used. However, their use is limited due to safety concerns, including potential side effects.

[0007] Therefore, there was a need to develop a health food composition made from natural substances that is harmless to the body and has excellent antioxidant properties.

[0008] The present invention aims to solve the problems of the prior art as described above by providing an antioxidant inner beauty health food composition made of a natural material that is harmless to the body and has excellent antioxidant efficacy, and a processed food and health functional food containing the same.

[0009] In addition, the present invention aims to provide a method for reducing oxidative stress by using the aforementioned antioxidant inner beauty health food composition, processed food or health functional food containing the same.

[0010] However, the problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned can be clearly understood by those skilled in the art from the description below.

[0011] In order to achieve the above-described purpose, the present invention provides an antioxidant inner beauty health food composition comprising a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, and a processed product of star anise as effective ingredients.

[0012] In the present invention, with respect to 100 parts by weight of the antioxidant inner beauty health food composition, 1 to 15 parts by weight of the processed Haematococcus pluvialis product, 1 to 15 parts by weight of the processed aloe vera product, 1 to 40 parts by weight of the processed red grape product, 1 to 70 parts by weight of the processed ginger product, and 1 to 70 parts by weight of the processed star anise product may be included.

[0013] In the present invention, the antioxidant inner beauty health food composition may further include amino acids.

[0014] In the present invention, the amino acid may include at least one selected from arginine, glutamic acid, and a combination thereof.

[0015] In the present invention, the arginine and glutamic acid may be included in a weight ratio of 1:5 to 5:1.

[0016] In the present invention, with respect to 100 parts by weight of the antioxidant inner beauty health food composition, the processed Haematococcus pluvialis product may be included in an amount of 1 to 5 parts by weight, the processed aloe vera product in an amount of 1 to 5 parts by weight, the processed red grape product in an amount of 10 to 20 parts by weight, the processed ginger product in an amount of 10 to 20 parts by weight, and the processed star anise product in an amount of 10 to 40 parts by weight, the arginine in an amount of 10 to 40 parts by weight, and the glutamic acid in an amount of 10 to 40 parts by weight.

[0017] In the present invention, with respect to 100 parts by weight of the antioxidant inner beauty health food composition, the processed Haematococcus pluvialis product may be included in an amount of 2 to 3 parts by weight, the processed aloe vera product in an amount of 2 to 3 parts by weight, the processed red grape product in an amount of 12 to 17 parts by weight, the processed ginger product in an amount of 12 to 17 parts by weight, the processed star anise product in an amount of 12 to 17 parts by weight, the arginine in an amount of 35 to 40 parts by weight, and the glutamic acid in an amount of 10 to 15 parts by weight.

[0018] In the present invention, the antioxidant inner beauty health food composition can exhibit an effect of reducing oxidative stress through free radical scavenging ability.

[0019] In addition, the present invention provides processed foods and health functional foods containing the antioxidant inner beauty health food composition.

[0020] In the present invention, the processed food and health functional food may further contain collagen.

[0021] In the present invention, the collagen content may be 0.0001 to 99.0 parts by weight for each 100 parts by weight of the inner beauty processed food for antioxidant use and the health functional food.

[0022] In the present invention, the inner beauty processed food and health functional food for antioxidant use may have a formulation selected from the group consisting of powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, infused teas, gums, candies, and health drinks.

[0023] Furthermore, the present invention provides a method for reducing oxidative stress, which comprises administering the antioxidant inner beauty health food composition, a processed food containing the same, or a health functional food to a subject in need thereof.

[0024] In the present invention, the antioxidant inner beauty health food composition, processed food or health functional food containing the same can exhibit an effect of reducing oxidative stress through free radical scavenging ability and reducing power in the subject.

[0025] In the present invention, the processed food or health functional food may further contain collagen.

[0026] In the present invention, the collagen content may be 0.0001 to 99.0 parts by weight per 100 parts by weight of the processed food or health functional food.

[0027] In the present invention, the processed food and health functional food may be selected from the group consisting of powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, infused teas, gums, candies, and health drinks.

[0028] According to one embodiment of the present invention, an antioxidant inner beauty health food composition can provide an antioxidant inner beauty health food composition comprising, as active ingredients, a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, and a processed product of star anise, which are known to have antioxidant effects.

[0029] According to one embodiment of the present invention, an antioxidant inner beauty health food composition can provide an antioxidant inner beauty health food composition comprising, as active ingredients, a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, a processed product of star anise, and an amino acid, which are known to have antioxidant effects.

[0030] An inner beauty processed food for antioxidants according to one embodiment of the present invention can improve antioxidant efficacy while being harmless to a living body by including the inner beauty health food composition for antioxidants.

[0031] An antioxidant inner beauty health functional food according to one embodiment of the present invention can provide an antioxidant inner beauty health functional food that is harmless to a living body and has excellent antioxidant efficacy and skin defense effects by including the antioxidant inner beauty health functional food composition.

[0032] Figure 1 shows the electron donating ability measured by FRAP of an antioxidant inner beauty health food composition containing five types of processed materials according to one embodiment of the present invention.

[0033] Figure 2 shows the electron donating ability measured by FRAP of an antioxidant inner beauty health food composition containing five types of processed materials and two types of amino acids according to one embodiment of the present invention.

[0034] Figure 3 shows the electron donating ability by ABTS of an antioxidant inner beauty health food composition containing five types of processed materials and two types of amino acids according to one embodiment of the present invention.

[0035] In this specification, when a part is said to "include" a certain component, this does not mean that it excludes other components, but rather that it may include other components, unless otherwise specifically stated.

[0036] In this specification, “A and / or B” means “A and B, or A or B.”

[0037] In this specification, “antioxidant” refers to inhibiting oxidation of cells by highly reactive free radicals or reactive oxygen species (ROS) due to oxidative stress caused by intracellular metabolism or ultraviolet rays, and includes reducing damage to cells caused by removing free radicals or reactive oxygen species.

[0038] Hereinafter, the present invention will be described in more detail.

[0039] The first aspect of the present invention relates to an antioxidant inner beauty health food composition comprising a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, and a processed product of star anise as active ingredients.

[0040] In this specification, "processed material" means any material obtained by physically and / or chemically treating a raw material. By applying conventional processing methods such as chopping, crushing, pulverizing, powdering, pressing, concentrating, fermenting, and extracting alone or in appropriate combination with other methods such as filtration, concentration, and drying, the chopping, crushing, pulverizing, powdering, pressing, concentrating, fermenting, and extracting used as the processed material of the present invention can be produced.

[0041] An inner beauty health food composition for antioxidant use according to one embodiment of the present invention is derived from natural materials, is harmless to the body, and has an excellent antioxidant effect, thereby preventing damage to substrate proteins that play a role in protecting the skin, thereby maintaining the tension, strength, and elasticity of the skin.

[0042] Haematococcus pluvialis is a unicellular green freshwater algae that can be found in Arctic snowfields, oceans, lakes, and other microalgae. According to one embodiment of the present invention, the processed Haematococcus pluvialis product may be prepared in the form of a powder formulation comprising astaxanthin and an extract dried and powdered. Astaxanthin is known to have antioxidant and skin protective properties.

[0043] In this specification, the term "extract" includes the extract itself and all formulations that can be formed using the extract, such as an extract obtained by extraction treatment of a raw material, a diluted or concentrated extract, a dried product obtained by drying the extract, a conditioned or purified product of the extract, or a mixture thereof.

[0044] In the present invention, the extract can be extracted from a natural, hybrid or variant plant used as a raw material, and can also be extracted from a plant tissue culture.

[0045] In this specification, the term "extraction" may mean taking something out of the whole.

[0046] In the present invention, the extract can be prepared using hexane, chloroform, methylene chloride, ethyl acetate, acetone, a lower alcohol of C1 to C4, water or a mixed solvent thereof as an extraction solvent, and preferably, it can be prepared using a lower alcohol of C1 to C4, water or a mixed solvent thereof as an extraction solvent, but is not limited thereto.

[0047] The method for preparing the above extract is not particularly limited, and extraction can be performed according to a method commonly used in the relevant technical field. Non-limiting examples of extraction methods include supercritical extraction, immersion extraction, hot water extraction, ultrasonic extraction, filtration, and reflux extraction, and these may be performed alone or in combination of two or more methods.

[0048] In the present invention, the extract can be manufactured by supercritically extracting the raw material at least once using a supercritical fluid (e.g., carbon dioxide, pentane, etc.), and can be manufactured as a dried extract obtained by concentrating the supercritical extract under reduced pressure and then freeze-drying or spray-drying it.

[0049] Aloe vera is a succulent plant species of the genus Aloe, and is an evergreen perennial plant. Although it originated in the Arabian Peninsula, it can grow wild in tropical, subtropical, and arid climates around the world. The aloe vera processed product according to one embodiment of the present invention may be a powder formulation obtained by freeze-drying aloe vera gel, including aloe sterol. The aloe sterol is known to have antioxidant and skin regeneration effects.

[0050] Grape is a general term for plants of the genus Grape or its fruit. It is a deciduous vine plant of the Grape family and its varieties include grape (Vitis vinifera), American grape (Vitis labrusca), and hybrids. Grape (V. vinifera) has been differentiated into cultivated types such as Southern European, Central Asian, and East Asian depending on the propagation process, and a total of 150,000 varieties have been created to date. The red grape processed product according to one embodiment of the present invention may be a powder formulation obtained through a concentration process after pressing red grapes, including resveratrol. Resveratrol is known to have antioxidant, skin defense, and skin regeneration effects.

[0051] Ginger (Zingiber officinale) is a perennial herb belonging to the Zingiberaceae family and Zingiberaceae order, a monocotyledonous plant. It is also called saengang or saeyang and is native to Southeast Asia. The processed ginger product according to one embodiment of the present invention may be in powder form and include 10-Shogaol, a substance converted from gingerol in dried ginger. 10-Shogaol is known to have antioxidant, skin protective, and skin regenerative effects.

[0052] Star anise (Illicium verum) is an evergreen plant belonging to the Schisandra chinensis family native to China. The dried fruit is also called star anise or star anise fragrant in China and Korea and is mainly used as a spice. It grows wild in the border region of Guangxi Zhuang Autonomous Region in southern China and northern Vietnam, and is widely cultivated in southern China, southern India, and Indochina. The star anise processed product according to one embodiment of the present invention contains shikimic acid and may be in powder form. The shikimic acid is known to have antioxidant and skin regeneration effects.

[0053] According to one embodiment of the present invention, the Haematococcus pluvialis processed product, the aloe vera processed product, the red grape processed product, the ginger processed product, and the star anise processed product may be obtained by separately processing according to the characteristics of each raw material. For example, the Haematococcus pluvialis processed product may be manufactured by supercritical extraction of Haematococcus pluvialis, drying and powdering the extract, the aloe vera processed product may be manufactured by freeze-drying aloe vera gel and then powdering it, the red grape processed product may be manufactured by pressing and concentrating red grapes and then powdering it, the ginger processed product may be manufactured by powdering dried ginger, and the star anise processed product may be manufactured by powdering dried star anise fruit, but is not limited thereto. When manufacturing a composite by mixing the above five types of processed materials obtained through separate manufacturing processes, not only can the efficacy of the raw materials be maintained, but the efficacy can also be enhanced compared to manufacturing a processed material after mixing the raw materials.

[0054] In the present invention, the processed product may be an extract from at least one selected from the group consisting of the whole plant, root, stem, branch, leaf, seed and fruit of Haematococcus pluvialis, aloe vera, red grape, ginger or star anise. Specifically, the processed product may be derived from all or part of Haematococcus pluvialis, aloe vera, red grape, ginger or star anise, and may be a ground product obtained by grinding each of these; a chopped product obtained by grinding; a pressed product obtained by pressing; a concentrated concentrate obtained by concentrating; a dried product obtained by drying all or part of Haematococcus pluvialis, aloe vera, red grape, ginger or star anise, the ground product or the chopped product; an extract obtained by extracting a specific component with a solvent from all or part of Haematococcus pluvialis, aloe vera, red grape, ginger or star anise; or a concentrate obtained by concentrating the extract.

[0055] According to one embodiment of the present invention, the inner beauty health food composition for antioxidants may contain 1 to 70 parts by weight of the processed ginger and 1 to 70 parts by weight of the processed star anise, based on 100 parts by weight of the composition. Specifically, the processed ginger may be contained in an amount of 5 to 65 parts by weight, 10 to 60 parts by weight, 10 to 55 parts by weight, 10 to 50 parts by weight, 10 to 45 parts by weight, 10 to 40 parts by weight, 10 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 11 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight, or 14 to 15 parts by weight, based on 100 parts by weight of the composition. Furthermore, the star anise processed product may be included in an amount of 5 to 65 parts by weight, 10 to 60 parts by weight, 10 to 55 parts by weight, 10 to 50 parts by weight, 10 to 45 parts by weight, 10 to 40 parts by weight, 10 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 11 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight, or 14 to 15 parts by weight, based on 100 parts by weight of the composition. By including the ginger processed product and the star anise processed product in an amount within the above numerical range, excellent antioxidant activity can be exhibited.

[0056] According to one embodiment of the present invention, the inner beauty health food composition for antioxidants may include 1 to 15 parts by weight of the processed Haematococcus pluvialis product, 1 to 15 parts by weight of the processed aloe vera product, 1 to 40 parts by weight of the processed red grape product, 1 to 70 parts by weight of the processed ginger product, and 1 to 70 parts by weight of the processed star anise product, with respect to 100 parts by weight of the composition. Specifically, the processed Haematococcus pluvialis product may be included in an amount of 1 to 14 parts by weight, 1 to 12 parts by weight, 1 to 10 parts by weight, 1 to 8 parts by weight, 1 to 6 parts by weight, 1 to 4 parts by weight, 2 to 4 parts by weight, or 2 to 3 parts by weight, with respect to 100 parts by weight of the composition. Furthermore, the aloe vera processed product may be included in an amount of 1 to 14 parts by weight, 1 to 12 parts by weight, 1 to 10 parts by weight, 1 to 8 parts by weight, 1 to 6 parts by weight, 1 to 4 parts by weight, 2 to 4 parts by weight, or 2 to 3 parts by weight, based on 100 parts by weight of the composition. In addition, the red grape processed product may be included in an amount of 5 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 11 to 19 parts by weight, 12 to 18 parts by weight, 13 to 17 parts by weight, or 14 to 16 parts by weight. In addition, the ginger processed product may be included in an amount of 5 to 65 parts by weight, 10 to 60 parts by weight, 10 to 55 parts by weight, 10 to 50 parts by weight, 10 to 45 parts by weight, 10 to 40 parts by weight, 10 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 11 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight, or 14 to 15 parts by weight, based on 100 parts by weight of the composition.Furthermore, the star anise processed product may be included in an amount of 5 to 65 parts by weight, 10 to 60 parts by weight, 10 to 55 parts by weight, 10 to 50 parts by weight, 10 to 45 parts by weight, 10 to 40 parts by weight, 10 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 11 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight, or 14 to 15 parts by weight, based on 100 parts by weight of the composition. By including the processed product in an amount within the above numerical range, the antioxidant inner beauty health food composition can realize an excellent antioxidant effect.

[0057] According to one embodiment of the present invention, the antioxidant inner beauty health food composition further comprises amino acids, and the amino acids may be arginine and glutamic acid, more preferably L-arginine and L-glutamic acid. By further comprising arginine and glutamic acid, the antioxidant activity of the antioxidant inner beauty health food composition may be increased, thereby enhancing the skin defense effect.

[0058] Arginine is an α-amino acid used in protein biosynthesis, and is known to have antioxidant enzyme activity, skin structure repair, and cell proliferation and growth effects.

[0059] Glutamic acid is an α-amino acid used in protein biosynthesis and is known to have the effect of restoring skin structure and promoting cell proliferation and growth.

[0060] According to one embodiment of the present invention, the antioxidant inner beauty health food composition may contain arginine and glutamic acid in a weight ratio of 5:1 to 1:5, preferably 3:1 to 1:3. More preferably, the arginine and glutamic acid may contain arginine and glutamic acid in a weight ratio of 2.34:1 to 4:1, specifically, 2.5:1 to 3.7:1, 2.7:1 to 3.5:1, 2.9:1 to 3.3:1, or 2.9:1 to 3.1:1. By containing arginine and glutamic acid in the weight ratio, the antioxidant activity of the antioxidant inner beauty health food composition may increase.

[0061] According to one embodiment of the present invention, the inner beauty health food composition for antioxidants may include a mixture of a processed Haematococcus pluvialis product, a processed aloe vera product, a processed red grape product, a processed ginger product, and a processed star anise product, and an amino acid in a weight ratio of 0.73:1 to 1.26:1, specifically, a weight ratio of 0.80:1 to 1.20:1, 0.90:1 to 1.10:1, or 0.95:1 to 1.00:1. By including the processed product and the amino acid in the weight ratio, excellent antioxidant and skin structure repair effects can be realized.

[0062] According to one embodiment of the present invention, the inner beauty health food composition for antioxidants may include, with respect to 100 parts by weight of the composition, 1 to 5 parts by weight of processed Haematococcus pluvialis, 1 to 5 parts by weight of processed aloe vera, 10 to 20 parts by weight of processed red grape, 10 to 20 parts by weight of processed ginger, 10 to 20 parts by weight of processed star anise, 10 to 40 parts by weight of arginine, and 10 to 40 parts by weight of glutamic acid.Specifically, the Haematococcus pluvialis processed product may be included in an amount of 2 to 4 parts by weight or 2 to 3 parts by weight with respect to 100 parts by weight of the composition, the aloe vera processed product may be included in an amount of 2 to 4 parts by weight or 2 to 3 parts by weight with respect to 100 parts by weight of the composition, the red grape processed product may be included in an amount of 11 to 19 parts by weight, 12 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight or 14 to 15 parts by weight with respect to 100 parts by weight of the composition, the ginger processed product may be included in an amount of 11 to 19 parts by weight, 12 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight or 14 to 15 parts by weight with respect to 100 parts by weight of the composition, and the star anise processed product may be included in an amount of 100 parts by weight of the composition, It may be included in 11 to 19 parts by weight, 12 to 18 parts by weight, 12 to 17 parts by weight, 13 to 16 parts by weight, or 14 to 15 parts by weight, and the arginine may be included in 15 to 40 parts by weight, 20 to 40 parts by weight, 25 to 40 parts by weight, 30 to 40 parts by weight, 35 to 40 parts by weight, 36 to 39 parts by weight, or 37 to 38 parts by weight, based on 100 parts by weight of the composition, and the glutamic acid may be included in 10 to 35 parts by weight, 10 to 30 parts by weight, 10 to 25 parts by weight, 10 to 20 parts by weight, 10 to 15 parts by weight, 11 to 14 parts by weight, or 12 to 13 parts by weight, based on 100 parts by weight of the composition. By including the above-mentioned processed material, arginine and glutamic acid in the above-mentioned numerical range of contents, the above-mentioned antioxidant inner beauty health food composition can realize excellent antioxidant and skin structure repair effects.

[0063] According to a preferred embodiment of the present invention, an inner beauty health food composition for antioxidants may include, with respect to 100 parts by weight of the composition, 2.5 parts by weight of a processed Haematococcus pluvialis product, 2.5 parts by weight of a processed aloe vera product, 15 parts by weight of a processed red grape product, 15 parts by weight of a processed ginger product, 15 parts by weight of a processed star anise product, 37.5 parts by weight of arginine, and 12.5 parts by weight of glutamic acid.

[0064] In a specific embodiment of the present invention, a FRAP (Ferric reducing antioxidant power) analysis was performed to evaluate the antioxidant activity of three compositions prepared according to the mixing ratios of Examples 4, 5, and 6 as described in Table 7. As a result, as shown in Table 8 and Fig. 2, all three compositions of Examples 4 to 6 exhibited high FRAP activity, confirming that they had excellent antioxidant activity.

[0065] Therefore, the inner beauty health food composition for antioxidant use according to the present invention can exhibit an effect of reducing oxidative stress through reducing power.

[0066] In another specific embodiment of the present invention, the ABTS radical scavenging activity of each composition was confirmed to evaluate the antioxidant activity of three compositions prepared according to the mixing ratios of Examples 4, 5, and 6 as described in Table 7. As a result, as shown in Table 9, Table 10, and Figure 3, the composition prepared according to the mixing ratio of Example 6 exhibited the strongest ABTS scavenging activity, and the ABTS IC 50 It was also confirmed that the value represents the lowest value.

[0067] Therefore, the inner beauty health food composition for antioxidant use according to the present invention can exhibit an effect of reducing oxidative stress through free radical scavenging ability.

[0068] Oxidative stress refers to a condition in which oxidizing substances, such as ROS, are excessively produced within the body or the antioxidant defense system is not functioning adequately, causing damage to biomolecules. It is associated with aging. Consequently, the antioxidant inner beauty health food composition according to the present invention can suppress or reduce oxidative stress through its superior reducing power and free radical scavenging ability, and thus can be used to prevent, improve, or treat cellular or skin aging.

[0069] The second aspect of the present invention relates to a processed food comprising the antioxidant inner beauty health food composition.

[0070] An inner beauty processed food for antioxidants according to one embodiment of the present invention can enhance antioxidant efficacy while being harmless to a living body by including the inner beauty health food composition for antioxidants.

[0071] According to one embodiment of the present invention, the content of the antioxidant inner beauty health food composition in the antioxidant inner beauty processed food may be 0.0001 to 99 parts by weight based on 100 parts by weight of the antioxidant inner beauty processed food. By adjusting the content of the antioxidant inner beauty health food composition in the antioxidant inner beauty processed food within the above-described range, the antioxidant efficacy can be improved while being harmless to the body.

[0072] According to one embodiment of the present invention, the inner beauty processed food for antioxidants may further comprise collagen, preferably low-molecular collagen, and more preferably low-molecular fish collagen. The average molecular weight of the low-molecular collagen may be 800 to 1,200 Da, but is not limited thereto.

[0073] As described above, by including more collagen in the inner beauty processed food for antioxidant purposes, the antioxidant effect can be improved while being harmless to the body.

[0074] According to one embodiment of the present invention, the content of the collagen in the inner beauty processed food for antioxidant purposes may be 0.0001 to 99 parts by weight based on 100 parts by weight of the inner beauty processed food for antioxidant purposes. By controlling the content of the collagen in the inner beauty processed food for antioxidant purposes within the above-described range, the antioxidant efficacy can be improved while being harmless to the body.

[0075] One embodiment of the present invention provides an antioxidant inner beauty health functional food comprising the antioxidant inner beauty health functional food composition.

[0076] An inner beauty health functional food for antioxidants according to one embodiment of the present invention can achieve excellent antioxidant effects and skin wrinkle improvement effects.

[0077] According to one embodiment of the present invention, the content of the antioxidant inner beauty health food composition in the antioxidant inner beauty health functional food may be 0.0001 to 99 parts by weight based on 100 parts by weight of the antioxidant inner beauty processed food. By adjusting the content of the antioxidant inner beauty health food composition in the antioxidant inner beauty health functional food within the above-described range, an excellent antioxidant effect and a skin wrinkle improvement effect can be realized.

[0078] According to one embodiment of the present invention, the antioxidant inner beauty health functional food may further comprise collagen. As described above, by further comprising collagen, the antioxidant inner beauty health functional food can enhance its antioxidant efficacy while remaining harmless to the body.

[0079] According to one embodiment of the present invention, the content of the collagen in the antioxidant inner beauty health functional food may be 0.0001 to 99 parts by weight based on 100 parts by weight of the antioxidant inner beauty health functional food. By adjusting the content of the collagen in the antioxidant inner beauty health functional food within the above-described range, an excellent antioxidant effect and skin wrinkle improvement effect can be achieved.

[0080] According to one embodiment of the present invention, the antioxidant inner beauty health functional food may have a formulation selected from the group consisting of powder, granule, tablet, capsule, pill, gel, jelly, suspension, emulsion, syrup, tea bag, infused tea, gum, candy, and health drink.

[0081] In this specification, “inner beauty” may be a general term for edible cosmetics that mean cultivating healthy skin from the inside, and “inner beauty food” may be an example of a health functional food.

[0082] In this specification, “health food” or “health functional food” may mean a general term for food that is effective in promoting health.

[0083] The above functional health food is the same term as food for special health use (FoSHU), and refers to a food with high medical and healthcare effects that is processed to efficiently exhibit a bioregulatory function in addition to providing nutrition. Here, "functionality" means regulating nutrients for the structure and function of the human body or obtaining a useful effect for health purposes such as physiological action. The food of the present invention can be manufactured by a method commonly used in the art, and during the manufacturing process, raw materials and ingredients commonly added in the art can be added. In addition, the formulation of the food can be manufactured without limitation as long as it is a formulation recognized as a food. The health functional food composition of the present invention can be manufactured in various forms of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long period of time because it uses food as a raw material.

[0084] The above health food or health functional food may contain conventional food additives, and the suitability of the food additives may be determined by the specifications and standards for the relevant item in accordance with the general provisions and general test methods of the Food Additive Code approved by the Ministry of Food and Drug Safety, unless otherwise provided for.

[0085] The items listed in the above food additive code include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon pigment, licorice extract, crystalline cellulose, high-molecular-weight pigment, and guar gum; and mixed preparations such as sodium L-glutamate preparations, alkaline agents for noodles, preservative preparations, and tar color preparations.

[0086] In one embodiment of the present invention, the composition can be used in various ways, and products that can be manufactured by containing the composition include various processed foods, health supplements, health functional foods, etc., and can be included in an amount of 0.0001 wt% to 99.0 wt% based on the total weight.

[0087] One example of a food containing the composition of the present invention may be a processed collagen food.

[0088] There are no specific restrictions on the types of the above foods. Examples of foods to which the above processed product can be added include formulations selected from the group consisting of powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, infused teas, gums, candies, and health drinks.

[0089] Specifically, the functional food in tablet form can be manufactured by granulating a mixture of the antioxidant inner beauty health food composition according to the present invention, collagen, excipients, binders, disintegrants, and other additives using a conventional method, then adding a lubricant, etc. and compression molding, or by directly compression molding the mixture. In addition, the functional food in tablet form can contain a maturing agent, etc., if necessary, and can also be coated with an appropriate coating agent, if necessary.

[0090] Among the functional foods in the form of capsules, hard capsules can be manufactured by filling a conventional hard capsule with a mixture of the antioxidant inner beauty health food composition according to the present invention, collagen, and excipients, or a granular or coated granular material thereof, and soft capsules can be manufactured by filling a mixture of the antioxidant inner beauty health food composition according to the present invention, collagen, and excipients, and additives, into a capsule base such as gelatin.

[0091] The above soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative, etc., as needed.

[0092] The above-mentioned ring-shaped functional food can be prepared by forming a mixture of the antioxidant inner beauty health food composition according to the present invention, collagen, excipients, binders, disintegrants, etc., by an appropriate method, and, if necessary, can be coated with white sugar or another appropriate coating agent, or can be coated with starch, talc, or an appropriate substance.

[0093] The above functional food in granular form can be manufactured into granules by a suitable method using a mixture of an antioxidant inner beauty health food composition according to the present invention, collagen, excipients, binders, disintegrants, etc., and can contain a flavoring agent, a flavoring agent, etc., as needed.

[0094] In addition, the definitions of terms for the above excipients, binders, disintegrants, lubricants, maturing agents, flavoring agents, etc. are those described in documents known in the art and may include those having the same or similar functions.

[0095] In addition to containing the antioxidant inner beauty health food composition and collagen as essential ingredients that can be included in the health food or health functional food of the present invention, there are no particular restrictions on other ingredients, and various herbal extracts, food additives, or natural carbohydrates, etc. can be contained as additional ingredients, just like in conventional foods.

[0096] The above health beverage composition may, like conventional beverages, contain various sweeteners, flavoring agents, or natural carbohydrates as additional ingredients. The sweetener may be a natural sweetener or a synthetic sweetener. The natural sweetener may be thaumatin or a stevia extract, and the synthetic sweetener may be saccharin or aspartame.

[0097] The natural carbohydrate may be a monosaccharide, a disaccharide, a polysaccharide, xylitol, sorbitol, or erythritol. The monosaccharide may be glucose or fructose, and the disaccharide may be maltose or sucrose. The polysaccharide may be dextrin or cyclodextrin.

[0098] The processed food or health functional food may include food additives that are food-scientifically acceptable, and may include an appropriate carrier commonly used in the manufacture of the processed food or health functional food.

[0099] In addition to the above, the composition of the present invention may include various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the composition of the present invention may include fruit pulp for the production of natural fruit juice, fruit juice drinks, and vegetable drinks. These ingredients may be used independently or in combination.

[0100] A third aspect of the present invention relates to a method for reducing oxidative stress, comprising administering an effective amount of the antioxidant inner beauty health food composition, a processed food containing the same, or a health functional food to a subject in need thereof.

[0101] The term "subject" as used herein refers to any animal, including humans, that requires antioxidant activity or that has been or is likely to be induced with oxidative stress. The animal may be, but is not limited to, mammals such as cows, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that require treatment for symptoms similar to those of humans.

[0102] As used herein, the term “administration” means introducing the antioxidant inner beauty health food composition of the present invention, a processed food or health functional food containing the same, to a subject by any appropriate method.

[0103] The antioxidant inner beauty health food composition of the present invention, and the processed food or health functional food containing the same, can be administered in an effective amount.

[0104] The above "effective amount" refers to an amount sufficient to achieve the efficacy or activity (e.g., antioxidant activity and reduction of oxidative stress) of the antioxidant inner beauty health food composition, processed food containing it, or health functional food. In the context of the present invention, the effective amount administered to a subject will depend on the subject's characteristics, such as general health, age, sex, and weight. Those skilled in the art can determine an appropriate dosage based on these and other factors.

[0105] The typical daily dosage of the antioxidant inner beauty health food composition according to the present invention, or the processed food or health functional food containing the same, can be appropriately selected by those skilled in the art, and can be administered once or in several divided doses.

[0106] The antioxidant inner beauty health food composition of the present invention, the processed food or health functional food containing the same, may be administered daily or intermittently, and the number of administrations per day may be once or divided into 2-3 times. In addition, the antioxidant inner beauty health food composition of the present invention, the processed food or health functional food containing the same, may be used alone or in combination with other antioxidants to reduce oxidative stress. Taking all of the above factors into consideration, it is important to administer an amount that achieves the maximum effect with the minimum amount without causing side effects, and this can be easily determined by those skilled in the art.

[0107] Hereinafter, preferred examples are presented to aid understanding of the present invention. However, the following examples are provided solely to facilitate understanding of the present invention and are not intended to limit the scope of the present invention.

[0108] [Manufacturing Example 1]

[0109] 1-1. Analysis of the components of each processed product and selection of mixing ratio

[0110] In order to manufacture an antioxidant inner beauty health food composition containing Haematococcus pluvialis processed product, aloe vera processed product, red grape processed product, ginger processed product, and star anise processed product as active ingredients, the components of each processed product were analyzed and a possible mixing ratio was derived.

[0111] In this specification, “mixing ratio” may mean “weight ratio” and may be expressed so that the sum is 1.

[0112] Specifically, the Haematococcus pluvialis product was manufactured by supercritical extraction of Haematococcus pluvialis, followed by drying and powdering the extract; the aloe vera product was manufactured by freeze-drying aloe vera gel and then powdering it; the red grape product was manufactured by pressing and concentrating red grapes and then powdering them; the ginger product was manufactured by powdering dried ginger; and finally, the star anise product was manufactured by powdering dried star anise fruits.

[0113] 1-2. Measurement of total polyphenol content in 5 types of processed products

[0114] The total polyphenol content (TPC) of each of the five types of processed products manufactured in the above Manufacturing Example 1-1 was confirmed as samples.

[0115] Specifically, the total polyphenol content was measured using the Folin-Denis method. After preparing gallic acid and each sample, 0.01 mL of the sample was mixed with 0.79 mL of 3-DW, 0.15 mL of 20% sodium carbonate solution was added, and mixed. 0.05 mL of 0.9 N Folin-ciocalteu was added, and the mixture was reacted in a dark room at room temperature for 30 minutes. 200 μL was dispensed into a microplate, and the absorbance was measured at 750 nm using a microplate reader (INNO-M, LTek, Korea). The average absorbance and mg / mL concentration of each gallic acid were plotted graphically to draw a standard curve, and the total polyphenol content of each sample was calculated, and the results are shown in Table 1 below (Haematococcus pluvialis, aloe vera, and red grape products were dissolved and used at a concentration of 100 mg / mL, and ginger and star anise products were dissolved and used at a concentration of 50 mg / mL).

[0116] Total polyphenol content (mg(GAE) / g) Haematococcus pluvialis processed product 1.63 ± 0.10 Aloe vera processed product 0 ± 0 Red grape processed product 1.579 ± 0.327 Ginger processed product 18.77 ± 0.47 Star anise processed product 30.06 ± 0.10

[0117] As shown in Table 1 above, it was confirmed that the total polyphenol content of processed ginger and processed star anise showed higher values ​​than other processed products. From these results, it can be expected that processed ginger and processed star anise will have a greater effect on antioxidant activity than other processed products.

[0118] [Manufacturing Example 2]

[0119] Measurement of total flavonoid content in five types of processed foods

[0120] The total flavonoid contents (TFC) of each of the five processed products manufactured in the above Manufacturing Example 1-1 were confirmed as samples. Specifically, after preparing catechin and each processed product sample, 50 μL of each sample was dispensed into a microplate, 30 μL of 5% NaNO2 was dispensed, mixed, and reacted in a dark room for 5 minutes. 60 μL of 2% AlCl3 was additionally dispensed, mixed, and reacted in a dark room for 6 minutes, and 100 μL of 4% NaOH was added, mixed, and reacted in a dark room for 11 minutes. When the reaction was completed, the absorbance was measured at 510 nm using a microplate reader (INNO-M, LTek, Korea). The average absorbance and mg / mL concentration of each catechin were plotted graphically to draw a standard curve, and the total flavonoid content of each sample was calculated. The results are shown in Table 2 below (Haematococcus pluvialis, aloe vera, and red grape products were dissolved and used at a concentration of 100 mg / mL, and ginger and star anise products were dissolved and used at a concentration of 10 mg / mL).

[0121] Total flavonoid content (mg(GAE) / g) Haematococcus pluvialis processed product 1.12 ± 0.004 Aloe vera processed product 0 ± 0 Red grape processed product 0.34 ± 0.003 Ginger processed product 7.64 ± 0.21 Star anise processed product 17.18 ± 0.72

[0122] As shown in Table 2 above, the total flavonoid content of processed ginger and processed star anise was confirmed to be higher than that of other processed products. From these results, it can be expected that processed ginger and processed star anise will have a greater effect on antioxidant activity than other processed products.

[0123] [Manufacturing Example 3]

[0124] Selection of the mixing ratio of 5 types of processed materials

[0125] The optimal mixing ratio of processed Haematococcus pluvialis, processed Aloe vera, processed red grape, processed ginger, and processed star anise was designed. Specifically, the appropriate mixing ratio of the above five types of processed products was designed using Response Surface Methodology (RSM). Response Surface Methodology is a type of statistical analysis method that predicts the result value at the level of a random variable belonging to the entire region of interest and optimizes the level of the variable to obtain the desired result value. In order to set the condition with the maximum antioxidant effect as the optimal condition, the independent variables for utilizing RSM were set as processed Haematococcus pluvialis, processed Aloe vera, processed red grape, processed ginger, and processed star anise.

[0126] Among the 36 mixing ratios obtained through response surface analysis, 6 mixing ratios including all 5 types of processed products and 1 mixing ratio in which the mixing ratios of the 5 types of processed products were appropriately adjusted to contain processed ginger and processed star anise in a certain ratio are shown in Table 3 below. Among these, 3 mixing ratios expected to have the best antioxidant effect were selected and used as examples.

[0127] Haematococcus pluvialis processed product, Aloe vera processed product, Red grape processed product, Ginger processed product, Star anise processed product, 10.60.10.10.10.120.10.60.10.10.130.10.10.60.10.140.10.10.10.60.150.10.10.10.10.660.20.20.20.270.050.050.30.30.3

[0128] [Example 1]

[0129] An antioxidant inner beauty health food composition was prepared by mixing Haematococcus pluvialis processed product, aloe vera processed product, red grape processed product, ginger processed product, and star anise processed product in a mixing ratio of 0.1:0.1:0.1:0.6:0.1.

[0130] [Example 2]

[0131] An antioxidant inner beauty health food composition was manufactured in the same manner as in Example 1, except that the mixing ratio was changed to 0.1:0.1:0.1:0.1:0.6.

[0132] [Example 3]

[0133] An antioxidant inner beauty health food composition was prepared in the same manner as in Example 1, except that the mixing ratio was changed to 0.05:0.05:0.3:0.3:0.3.

[0134] [Comparative Example 1]

[0135] An antioxidant inner beauty health food composition was manufactured in the same manner as in Example 1, except that the mixing ratio was changed to 0.25:0.25:0.25:0.25:0.

[0136] [Comparative Example 2]

[0137] An antioxidant inner beauty health food composition was manufactured in the same manner as in Example 1, except that the mixing ratio was changed to 0.25:0.25:0.25:0:0.25.

[0138] [Comparative Example 3]

[0139] An inner beauty health food composition for antioxidants was manufactured in the same manner as in Example 1, except that the mixing ratio was changed to 0.25:0.25:0:0.25:0.25.

[0140] [Comparative Example 4]

[0141] An antioxidant inner beauty health food composition was manufactured in the same manner as in Example 1, except that the mixing ratio was changed to 0.25:0:0.25:0.25:0.25.

[0142] [Comparative Example 5]

[0143] An antioxidant inner beauty health food composition was prepared in the same manner as in Example 1, except that the mixing ratio was changed to 0:0.25:0.25:0.25:0.25.

[0144] [Example 1]

[0145] Measurement of electron donating ability by ABTS

[0146] The electron donating ability of the inner beauty health food compositions for antioxidants manufactured in Examples 1 to 3 and Comparative Examples 1 to 5 by ABTS was measured. Specifically, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline6-sulfonic acid) radical scavenging activity is a method of measuring the degree to which a blue-green ABTS solution changes to colorless when a cationic radical generated by the reaction between ABTS and potassium persulfate is scavenged by an antioxidant substance, using absorbance.

[0147] ABTS reagent was prepared by diluting 7 mM ABTS (2,2'-Azino-bis(3-etl1ylbenzothiazaline-6-sulfonic acid) Diammonium salt) and 2.45 mM potassium persulfate solution in a 1:1 ratio and storing in a dark room for 24 hours before use. The sample was dissolved in DW at a concentration of 1 mg / mL and used. 10 μL of the sample was dispensed into a 96-well plate, followed by 200 μL of ABTS reagent and mixing. After reacting for 30 minutes in a dark room, the absorbance of the reaction solution was measured at 414 nm. Ascorbic acid was used as the control, and the ABTS radical scavenging activity was expressed as ascorbic acid equivalent (AAE) by converting the difference in absorbance between the reaction solution and the control into a percentage, and the standard deviation (SD) is also shown in Table 4 below.

[0148] AAE (mg / g) SD Example 122.37 1.14 Example 220.8 30.10 Example 330.79 2.52 Comparative Example 111.8 20.77 Comparative Example 210.90 0.62 Comparative Example 317.4 21.29 Comparative Example 419.7 32.66 Comparative Example 515.4 9 0.48

[0149] As shown in Table 4 above, it can be confirmed that Examples 1 to 3 exhibit superior antioxidant efficacy compared to Comparative Examples 1 to 5, with ABTS radical scavenging activity of 20 AAE (mg / g) or more.

[0150] [Example 2]

[0151] Measurement of electron donating ability by FRAP

[0152] The electron donating ability of the inner beauty health food compositions for antioxidants prepared in Examples 1 to 3 and Comparative Examples 1 to 5 was measured by FRAP. Specifically, the ferric reducing / antioxidant power (FRAP) activity (reducing power) was measured by the oxidation of ferric cations (Fe) by an antioxidant at low pH. 3+ ) is a divalent iron cation (Fe 2+ ) is a method for measuring the sulfur oxidation activity of a sample by using the characteristic of being reduced to .

[0153] The FRAP reagent was used in a 10:1:1 dilution of 10 mM TPTZ and 20 mM iron(III) chloride hexahydrate (FeCl36H2O) dissolved in 300 mM acetate buffer (pH 3.6) and 40 mM HCl, and the sample was used dissolved in DMSO at a concentration of 1 mg / mL. After dispensing 10 μL of each sample into a microplate, 200 μL of the FRAP reagent was dispensed and mixed, and the reaction was performed at 37°C for 5 minutes. The absorbance was measured at 593 nm using a microplate reader (INNO-M, LTek, Korea). Ascorbic acid was used as the control group, and the average absorbance and μg / mL concentration of ascorbic acid were plotted graphically to create a standard curve. The difference in absorbance between the non-added and added sample groups was expressed as mg ascorbic acid (AAE) per 1 g of sample, as shown in Table 5 below.

[0154] AAE (mg / g) SD Example 115.24 0.90 Example 221.99 0.47 Example 334.50 1.30 Comparative Example 12.00 0.02 Comparative Example 24.95 0.17 Comparative Example 35.72 0.16 Comparative Example 45.80 0.17 Comparative Example 55.67 0.18

[0155] Figure 1 shows the electron donating ability measured by FRAP of each antioxidant inner beauty health food composition including five types of processed products according to Examples 1 to 3 and Comparative Examples 1 to 5.

[0156] As shown in Table 5 and Figure 1 above, it was confirmed that Examples 1 to 3 had superior antioxidant activity effects compared to Comparative Examples 1 to 5.

[0157] In particular, in the case of Example 3, it was confirmed that the total content of processed ginger and processed star anise was lower than in Examples 1 and 2, but the antioxidant activity was similar to or superior to that of Examples 1 and 2.

[0158] [Example 3]

[0159] Selection of the mixing ratio of two types of amino acids and mixing with five types of processed products

[0160] In order to manufacture a composition containing two types of amino acids, arginine and glutamic acid, in addition to the antioxidant inner beauty health food composition containing the five types of processed products of Example 3, the mixing ratio of the two types of amino acids was selected. Specifically, the appropriate mixing ratio of the two types of amino acids was designed using response surface methodology (RSM). In order to set the conditions for maximum antioxidant efficacy as the optimal conditions, the independent variables for utilizing response surface methodology were set to arginine and glutamic acid.

[0161] The specific mixing ratio is shown in Table 6 below.

[0162] Arginine glutamic acid 10.25 0.75 20.50 530.75 0.25

[0163] The mixing ratio of the antioxidant inner beauty health food composition containing the five types of processed materials of Example 3 and the composition containing the two types of amino acids mixed in a 1:1 ratio is shown in Table 7 below.

[0164] Haematococcus pluvialis processed product Aloe vera processed product Red grape processed product Ginger processed product Star anise processed product Arginine glutamic acid Example 40.0250.0250.150.150.150.1250.375 Example 50.0250.0250.150.150.150.250.25 Example 60.0250.0250.150.150.150.3750.125

[0165] [Example 4]

[0166] Measurement of electron donating capacity of a composition mixed with five types of processed materials and two types of amino acids by FRAP

[0167] The electron donating ability of five types of processed materials and two types of amino acids mixed with different mixing ratios in Table 7 was measured by FRAP and is shown in Table 8 and Fig. 2.

[0168] AAE (mg / mL) Example 4 16.34 ± 0.2 Example 5 16.00 ± 0.15 Example 6 16.450 ± 0.2

[0169] Figure 2 shows the electron donating ability measured by FRAP of each antioxidant inner beauty health food composition containing five types of processed materials and two types of amino acids according to Examples 4 to 6. As shown in Table 8 and Figure 2, there is no significant difference in the FRAP activity of Examples 4 to 6, but it can be confirmed that Example 6 exhibits superior antioxidant activity compared to Examples 4 and 5.

[0170] [Example 5]

[0171] Measurement of electron donating ability of a composition mixed with five types of processed materials and two types of amino acids by ABTS

[0172] The electron donating ability by ABTS of five types of processed materials and two types of amino acids mixed with different mixing ratios in Table 7 was measured and shown in Table 9 and Fig. 3.

[0173] AAE (mg / mL) Example 47.90 ± 0.42 Example 513.43 ± 0.93 Example 625.05 ± 0.19

[0174] Figure 3 shows the electron donating ability by ABTS of an antioxidant inner beauty health food composition containing five types of processed materials and two types of amino acids according to Examples 4 to 6.

[0175] As shown in Table 9 and Figure 3, it can be confirmed that Example 6 exhibits superior antioxidant activity compared to Examples 4 and 5, and it can be confirmed that the higher the mixing ratio of arginine in the composition, the higher the antioxidant activity.

[0176] ABTS IC of compositions containing five types of processed materials and two types of amino acids with different mixing ratios in Table 7 50 It is shown in Table 10 below.

[0177] ABTS IC 50 It refers to the sample concentration at which the cation radical scavenging rate is 50%, and the lower the value, the higher the antioxidant capacity.

[0178] ABTS IC 50 (mg / mL) Example 48.585 ± 0.65 Example 57.652 ± 0.35 Example 63.605 ± 0.28

[0179] As shown in Table 10, ABTS IC of Example 6 50 It can be confirmed that the value shows the best antioxidant activity as it shows a lower value compared to Examples 4 and 5, and it can be confirmed that the antioxidant activity also increases as the mixing ratio of arginine in the composition increases.

[0180] While specific aspects of the present invention have been described in detail above, it will be apparent to those skilled in the art that these specific descriptions merely represent preferred embodiments and are not intended to limit the scope of the present invention. Therefore, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antioxidant inner beauty health food composition comprising a processed product of Haematococcus pluvialis, a processed product of aloe vera, a processed product of red grapes, a processed product of ginger, and a processed product of star anise as active ingredients.

2. An antioxidant inner beauty health food composition according to claim 1, comprising 1 to 15 parts by weight of the processed Haematococcus pluvialis product, 1 to 15 parts by weight of the processed aloe vera product, 1 to 40 parts by weight of the processed red grape product, 1 to 70 parts by weight of the processed ginger product, and 1 to 70 parts by weight of the processed star anise product, with respect to 100 parts by weight of the antioxidant inner beauty health food composition.

3. An antioxidant inner beauty health food composition according to claim 1, further comprising amino acids, wherein the amino acids are arginine and glutamic acid.

4. An antioxidant inner beauty health food composition comprising arginine and glutamic acid in a weight ratio of 1:5 to 5:1 in the third paragraph.

5. In the third paragraph, an antioxidant inner beauty health food composition comprising, with respect to 100 parts by weight of the antioxidant inner beauty health food composition, 1 to 5 parts by weight of the processed Haematococcus pluvialis product, 1 to 5 parts by weight of the processed aloe vera product, 10 to 20 parts by weight of the processed red grape product, 10 to 20 parts by weight of the processed ginger product, and 10 to 20 parts by weight of the processed star anise product, 10 to 40 parts by weight of the arginine, and 10 to 40 parts by weight of the glutamic acid.

6. In the fifth paragraph, an antioxidant inner beauty health food composition comprising 2 to 3 parts by weight of the processed Haematococcus pluvialis product, 2 to 3 parts by weight of the processed aloe vera product, 12 to 17 parts by weight of the processed red grape product, 12 to 17 parts by weight of the processed ginger product, 12 to 17 parts by weight of the processed star anise product, 35 to 40 parts by weight of the arginine, and 10 to 15 parts by weight of the glutamic acid, with respect to 100 parts by weight of the antioxidant inner beauty health food composition.

7. In the first paragraph, the antioxidant inner beauty health food composition exhibits an effect of reducing oxidative stress through free radical scavenging ability and reducing power.

8. An antioxidant inner beauty health functional food comprising an antioxidant inner beauty health functional food composition according to Article 1.

9. An inner beauty health functional food for antioxidants further comprising collagen in the 8th paragraph.

10. In the 9th paragraph, the collagen content is 0.0001 to 99.0 parts by weight for 100 parts by weight of the antioxidant inner beauty processed food.

11. In the 8th paragraph, the antioxidant inner beauty health food has a formulation selected from the group consisting of powder, granule, tablet, capsule, pill, gel, jelly, suspension, emulsion, syrup, tea bag, infused tea, gum, candy, and health drink.

12. A method for reducing oxidative stress, comprising administering an effective amount of the antioxidant inner beauty health food composition of paragraph 1 to an individual in need thereof.

13. A method for reducing oxidative stress, wherein the antioxidant inner beauty health food composition in claim 12 exhibits an effect of reducing oxidative stress through free radical scavenging ability and reducing power in the subject.

14. A method for reducing oxidative stress, comprising administering an effective amount of the antioxidant inner beauty health functional food of Article 8 to an individual in need thereof.

15. A method for reducing oxidative stress in claim 14, wherein the antioxidant inner beauty health functional food further contains collagen.

16. A method for reducing oxidative stress, wherein, in paragraph 15, the collagen content is 0.0001 to 99.0 parts by weight for 100 parts by weight of the inner beauty processed food for antioxidant use.

17. A method for reducing oxidative stress in claim 14, wherein the antioxidant inner beauty health food has a formulation selected from the group consisting of powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, infused teas, gums, candies, and health drinks.

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