Combinations of polypeptide having dnase activity and soil release polymer for use in laundry detergent compositions

Abstract

The use of one or more polypeptides having DNase activity and one or more soil release polymers in a laundry process for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile is described. Furthermore, laundry detergent compositions comprising, as the DNase component, one or more polypeptides having DNase activity with at least 80% sequence identity to SEQ ID NO: 1, 2, 3, 4 or 5, and, as the soil release polymer component, one or more terephthalic acid-based soil release polymers are also disclosed.

Description

[0001] Combinations of polypeptide having DNase activity and soil release polymer for use in laundry detergent compositions

[0002] Field of the invention

[0003] The invention relates to the use of a polypeptide having DNase activity and a soil release polymer in a laundry process for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile, and to laundry detergent compositions comprising one or more polypeptides having DNase activity and one or more terephthalic acid-based soil release polymers.

[0004] Background of the invention

[0005] Laundry detergent compositions that lead, upon application in a laundry process, to a reduction of malodor redeposition on a textile, to a removal of malodor from a textile or to the maintenance or improvement of whiteness of a textile are already known. However, there is still a need for laundry detergent compositions which demonstrate all of the above-mentioned properties and / or show an improvement in one or more of the respective properties.

[0006] Therefore, it was an object of the present invention to provide ingredients or combinations of ingredients for laundry detergent compositions which lead to an advantageous reduction of malodor redeposition on a textile, to a beneficial removal of malodor from a textile and / or to an advantageous maintenance or improvement of whiteness of a textile when applied in a laundry process.

[0007] Surprisingly, it has been found that this problem is solved by the combined use of a polypeptide having DNase activity and a soil release polymer in a laundry process.

[0008] Therefore, a subject matter of the present invention is the use of a polypeptide having DNase activity and a soil release polymer in a laundry process for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile.

[0009] The combined use of a polypeptide having DNase activity and a soil release polymer in a laundry process leads to an advantageous reduction of malodor redeposition on textiles, to a beneficial removal of malodor from textiles and / or to an advantageous maintenance or improvement of whiteness of textiles. More particularly, the combination of a polypeptide having DNase activity and a soil release polymer has been found to provide a synergistic effect on malodor and whiteness compared to use of either a polypeptide having DNase activity or a soil release polymer alone, which is illustrated in the examples.

[0010] WO 2015 / 181286 A1 e.g. discloses the use of a polypeptide having deoxyribonuclease (DNase) activity for preventing or reducing redeposition of soil on an item during a subsequent cleaning or laundering process, a detergent composition comprising a polypeptide having deoxyribonuclease (DNase) activity and a method for preventing or redeposition of soil on an item during a subsequent cleaning or laundering process.

[0011] WO 2022 / 083538 A1 e.g. describes the use of a polypeptide having deoxyribonuclease (DNase) activity for preventing, reducing and / or removing malodor from a porous item, a detergent composition comprising said polypeptide and a method for cleaning a porous item soiled with a biofilm and / or a food stain.

[0012] WO 2022 / 189521 A1 e.g. discloses polypeptide variants having DNase activity and detergent compositions comprising the polypeptide variants, as well as methods for obtaining the variants, polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods using the variants.

[0013] WO 2017 / 064269 A1 e.g. describes polypeptide variants having DNase activity and methods obtaining the variants. It also describes polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variants.

[0014] WO 2015 / 155350 A1 e.g. discloses a detergent and a pharmaceutical composition comprising a deoxyribonuclease (DNase), wherein the DNase is obtained from a fungal source. It furthermore discloses a laundering method and the use of DNase, polypeptides having DNase activity, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides.

[0015] WO 2023 / 064749 A1 e.g. describes fabric and home care products comprising cationic soil release polymer and a lipase enzyme. The fabric and home care products show good whiteness performance.

[0016] WO 2017 / 214240 A2 e.g. discloses cleaning compositions having an enzyme system, where the enzyme system includes a nuclease enzyme, an extracellular-polymer-degrading enzyme, and a cleaning adjunct, methods of making and using such cleaning compositions and the use of an extracellular-polymer-degrading enzyme. Overview of Sequence Listing

[0017] SEQ ID NO: 1 DNase polypeptide from Aspergillus oryzae

[0018] SEQ ID NO: 2 DNase polypeptide from Aspergillus oryzae with truncated N-terminal

[0019] SEQ ID NO: 3 DNase polypeptide from Aspergillus oryzae with truncated N-terminal

[0020] SEQ ID NO: 4 DNase polypeptide from Metabacillus indicus (formerly Bacillus cibi)

[0021] SEQ ID NO: 5 DNase polypeptide, variant of SEQ ID NO: 4

[0022] SEQ ID NO: 6 nuclease polypeptide from Trichoderma reesei

[0023] SEQ ID NO: 7 nuclease polypeptide from Tolypocladium inflatum

[0024] SEQ ID NO: 8 nuclease polypeptide from Blastomyces gilchristii

[0025] SEQ ID NO: 9 nuclease polypeptide from Gelasinospora tetrasperma

[0026] SEQ ID NO: 10 nuclease polypeptide from Streptococcus dysgalactiae

[0027] SEQ ID NO: 11 nuclease polypeptide from Thermobifida cellulosilytica

[0028] SEQ ID NO: 12 nuclease polypeptide from Rasamsonia composticola

[0029] SEQ ID NO: 13 nuclease polypeptide from Chaetosartorya cremea

[0030] SEQ ID NO: 14 nuclease polypeptide from Aspergillus bisporus

[0031] SEQ ID NO: 15 nuclease polypeptide from Aspergillus clavatus

[0032] SEQ ID NO: 16 nuclease polypeptide from Metarhizium acridum

[0033] SEQ ID NO: 17 nuclease polypeptide from Aspergillus ochraceoroseus

[0034] Definitions

[0035] The term “removing malodor from textiles” in the context of the invention consists of two different terms “malodor removal from primary wash” and “malodor repellent”.

[0036] “Malodor removal from primary wash” refers to the fact that malodor on fabrics is removed by washing malodorous fabrics with a laundry detergent composition.

[0037] “Malodor repellent” refers to the fact that a malodor repellent film is formed on fabrics whilst the fabrics are washed with a laundry detergent composition. This malodor repellent film enables malodor on fabric to be washed off easily in a subsequent wash process with a laundry detergent composition.

[0038] The term “reducing redeposition of malodor on a textile” in the context of the invention refers to the fact that malodor, which is washed off from malodorous fabrics in a wash process using a laundry detergent composition, cannot redeposit from the wash liquor onto the fabric in the same wash process, or that there is a reduced level of malodor redeposition. "Soil release” polymer as used herein means a polymer that enhances soil removal during laundering by modifying the surface of the fabric that is laundered, preferably by increasing surface polarity.

[0039] The term “DNase” means a polypeptide having DNase (deoxyribonuclease) activity that catalyzes the hydrolytic cleavage of phosphodiester linkages in DNA, thus degrading DNA. DNases belong to the esterases (EC number 3.1), a subgroup of the hydrolases. The DNases may be classified in several subclasses, in particular EC 3.1.21 and its subclasses. The term “DNase” as used herein is also intended to include nucleases that cleave both RNA and DNA, for example the endoribonucleases in EC 3.1.30 that are active on both RNA and DNA. For purposes of the present invention, DNase activity may be determined according to the procedure described in Assay I. The terms “DNase” and “a polypeptide with DNase activity” may be used interchangeably throughout the application.

[0040] The term “variant” means a polypeptide having DNase activity comprising at least one alteration, i.e. a substitution, an insertion (including extension), and / or a deletion (e.g., truncation), at one or more positions. A substitution means replacement of the amino acid occupying a position with a different amino acid; a deletion means removal of the amino acid occupying a position; and an insertion means adding 1-5 amino acids (e.g., 1-3 amino acids, in particular one amino acid) adjacent to and immediately following the amino acid occupying a position. The alterations in a DNase variant are typically substitutions.

[0041] Sequence identity: The relatedness between two amino acid sequences is described by the parameter “sequence identity”.

[0042] For purposes of the present invention, the sequence identity between two amino acid sequences is determined as the output of “longest identity” using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 6.6.0 or later. The parameters used are a gap open penalty of 10, a gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. In order for the Needle program to report the longest identity, the -nobrief option must be specified in the command line. The output of Needle labeled “longest identity” is calculated as follows:

[0043] (Identical Residues x 100) / (Length of Alignment - Total Number of Gaps in Alignment) It is known that a host cell may produce a mixture of two of more different mature polypeptides, and that different host cells process polypeptides differently. For example, in the case of the mature polypeptide shown in SEQ ID NO: 1 , it has been found that this may be expressed with different N-terminal truncations, for example as the polypeptide of SEQ ID NO: 2 (206 amino acid residues, corresponding to amino acids 16 to 221 of SEQ ID NO: 1) or SEQ ID NO: 3 (204 amino acid residues, corresponding to amino acids 18 to 221 of SEQ ID NO: 1). Since SEQ ID NOs: 1 , 2 and 3 are identical other than truncation at the N-terminal, any reference herein to a polypeptide having a given percent sequence identity to SEQ ID NO: 1 should be understood as also including polypeptides having that percent sequence identity to SEQ ID NO: 2 or to SEQ ID NO: 3.

[0044] The term “wash liquor” refers to an aqueous solution comprising a polypeptide having DNase activity and a soil release polymer. A wash liquor is a solution, e.g. found in a washing machine, containing water and a detergent composition comprising the polypeptide having DNase activity and the soil release polymer. The detergent composition, prior to being mixed with water to form a wash liquor, may be in any form as described elsewhere herein, for example a liquid or powder.

[0045] Detailed description

[0046] In a preferred embodiment of the invention, the inventive use concerns the use of a polypeptide having DNase activity and a soil release polymer in a laundry process for reducing redeposition of malodor on a textile

[0047] In another preferred embodiment of the invention, the inventive use concerns the use of a polypeptide having DNase activity and a soil release polymer in a laundry process for removing malodor from a textile.

[0048] In another preferred embodiment of the invention, the inventive use concerns the use of a polypeptide having DNase activity and a soil release polymer in a laundry process for maintaining or improving whiteness of a textile.

[0049] In a further embodiment, the invention concerns a method for laundering a textile, comprising exposing the textile to a wash liquor comprising a polypeptide having DNase activity and a soil release polymer, completing at least one wash cycle, and optionally rinsing the textile.

[0050] In a still further embodiment, the invention concerns a method for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile, comprising exposing the textile to a wash liquor comprising a polypeptide having DNase activity and a soil release polymer, completing at least one wash cycle, and optionally rinsing the textile.

[0051] The polypeptide having DNase activity is typically of microbial origin, from a bacterium or a fungus. This includes both wildtype DNase polypeptides of bacterial or fungal origin as well as variants of such DNase polypeptides, where the variants have been engineered to have one or more improved property compared to the wildtype polypeptide. Such improved properties may, for example, be improved stability and / or improved wash performance. Improved stability includes improved detergent stability, i.e. improved stability during storage in a detergent composition. Improved wash performance includes improved removal or reduction of malodor and / or improved whiteness. The improved property of a DNase variant may be determined compared to the wildtype DNase or to a corresponding DNase without the alterations in the variant.

[0052] The polypeptide having DNase activity is in one embodiment a polypeptide having at least 60% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3. In particular, the polypeptide having DNase activity may have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 1 , 2 or 3.

[0053] In one embodiment, the polypeptide having DNase activity is a variant of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, wherein the variant has at least 60% but less than 100% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3. The variant may, for example, have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% or at least 95% sequence identity to SEQ ID NO: 1, 2 or 3.

[0054] Preferably, the variant has at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% or at least 95% sequence identity to SEQ ID NO: 1 , 2 or 3.

[0055] In another embodiment, the polypeptide having DNase activity is a polypeptide having at least 60% sequence identity to SEQ ID NO: 4. In particular, the polypeptide having DNase activity may have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4.

[0056] In one embodiment, the polypeptide having DNase activity is a variant of SEQ ID NO: 4, wherein the variant has at least 70% sequence identity to SEQ ID NO: 5. The variant of this embodiment preferably has at least 75%, at least 80%, at least 85% or at least 90% sequence identity to SEQ ID NO: 5. The variant may e.g. have at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 5.

[0057] A polypeptide having DNase activity and which is a variant of SEQ ID NO: 1 , 2 or 3, or a variant of SEQ ID NO: 4, may have any of the alterations outlined below. The following nomenclature is used in relation to the polypeptide variants described herein:

[0058] Substitutions. For an amino acid substitution, the following nomenclature is used: Original amino acid, position, substituted amino acid. Accordingly, the substitution of threonine at position 226 with alanine is designated as “T226A” Multiple mutations may be separated by addition marks (“+”), e.g., “G205R + S411 F”, representing substitutions at positions 205 and 411 of glycine (G) with arginine (R) and serine (S) with phenylalanine (F), respectively. Multiple mutations may alternatively be indicated by a space or a comma, e.g. “G205R S411F” or “G205R, S411F”.

[0059] Deletions. For an amino acid deletion, the following nomenclature is used: Original amino acid, position, *. Accordingly, the deletion of glycine at position 195 is designated as “G195*”. Multiple deletions are separated by addition marks (“+”), e.g., “G195* + S411*”.

[0060] Different alterations. Where different alternative substitutions can be introduced at a position, the different substitutions are separated by a comma, e.g., “R170Y,E” represents a substitution of arginine at position 170 with either tyrosine or glutamic acid. Similarly, “Y167G.A + R170G.A” designates the following variants: “Y167G+R170G”, “Y167G+R170A”, “Y167A+R170G” and “Y167A+R170A”.

[0061] Amino acid position numbers of DNase variants of SEQ ID NO: 1 , 2 or 3 as used herein are based on the numbering of SEQ ID NO: 1. Amino acid position numbers of DNase variants of SEQ ID NO: 4 as used herein are based on the numbering of SEQ ID NO: 4.

[0062] In some embodiments, the polypeptide having DNase activity is a variant of SEQ ID NO: 1 , 2 or 3, wherein the variant has at least 80% but less than 100% sequence identity to SEQ ID NO: 1, 2 or 3, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% or at least 95% sequence identity to SEQ ID NO: 1 , 2 or 3.

[0063] In one such embodiment, a polypeptide having DNase activity is a variant of SEQ ID NO: 1, 2 or 3 comprising one or more substitutions selected from the group consisting of N4E, L17E, T19A, T19G, T19I, K36P, Q38P, S39V, S39R, A40P, A40H, L41T, L41 H, V45H, L51G, K53T, K53P, G54P, A55P, N57H, E64A, E64Q, E64R, E64T, E64I, E64S, T66H, K67A, K67T, N68V, N68P, N68I, N68H, S69A, S69D, S69E, S69K, S69L, S69W, S69Y, S69Q, N70T, N70H, N70G, R71T, D72E, S74H, S74G, G75I, N77T, K82P, K82I, D83T, D83P, D83I, D83H, D83G, P84H, Q85T, Q85P, Q85H, K86T, K86P, K86H, G88P, G88H, A91 P, W99T, A101W, K105E, K105N, K105T, K105D, S106T, S115T, L116I, Q135L, G136L, V138I, V138L, V138P, V138Q, L139A, N140R, N140L, N140A, G141L, F151 R, D152Y, D152L, D152I, D152A, P153E, S154R, T162R, W163E, F164R, I166Y, I166R, K168N, F169R, F169E, A173I, A173R, A173T, S182R, N183E, D184I, K185Y, S186I, D189G, D189H, K212G, K212P and K215I. The variant may e.g. comprise one, two, three, four, five or more of said substitutions.

[0064] In another embodiment, a polypeptide having DNase activity is a variant of SEQ ID NO: 1 , 2 or 3 comprising two or more alterations selected from the group consisting of S69V, Q102E, S26*, D32Q, K36C,H, G37R, F43W, D46G, A55I, N68D, A76I, K82S,T, P84D,T, K86G,L,N,Q,T,V,Y, A91 R, L92E, K95I, P97E,N, A101E, K105N,G,Q,T,D, F112Y.W, L129K, N133Q, V138C, N140H, G141Q.R, S144E, N146A, K147N.E, V148I, A149D,E,F, Q150D, P153D.V, S154E, K155E,F,L,S,T, Q157D.E, Q158D, T159Q, K160D, T170Q, A172D,E,H,R, K185*, V187N.Y, N191*, K192A, D197K.S, G199Q, Q208V, E211Y,T,P, N213S, N214D, N217A and Y218D.E, for example three, four, five or more of said alterations.

[0065] In a further embodiment, a polypeptide having DNase activity is a variant of SEQ ID NO: 1, 2 or 3 comprising at least two substitutions, such as at least three substitutions, selected from the group consisting of A111 P, D32E, V35I, S69V, Q102E, K105N and G181 N. The DNase variant of this embodiment may for example comprise the substitution A111 P and two or more substitutions selected from the group consisting of S26H, D32E, V35I, K65E, K67A, S69E, S69V, S98R, Q102E, K105N, S115T, Q150E, Q157E, T159Q, G161R, A172E, G181N, S182Y, S182V, K185E, V187N, K192I, A206G, Q208V and K212E. An example of such a variant is one comprising the substitutions D32E, V35I, K65E, K67A, K105N, A111P, G181N and S182Y.

[0066] In some preferred embodiments, the polypeptide having DNase activity is a variant of SEQ ID NO: 4, wherein the variant has at least 80% sequence identity to SEQ ID NO: 5, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 5.

[0067] In one such embodiment, the variant of SEQ ID NO: 4 having DNase activity and having at least 80% sequence identity to SEQ ID NO: 5 is a variant comprising one or more substitutions selected from the group consisting of T1I, T1V, T1Y, T1M, T1E, G4N, T5F, T5C, P6V, P6G, S7D, S7T, K8V, S9K, S9Q, S9V, S9L, S9F, S9P, S9R, A10D, A10M, A10I, A10Q, A10V, A10L, A10K, Q12S, Q12V, Q12E, S13D, S13Y, S13Q, S13F, S13R, S13V, S13N, S13H, S13M, S13W, S13K, S13L, S13E, Q14M, Q14R, N16S, A17C, A17V, A17E, A17T, T19K, T19L, T19S, T19I, T19V, K21 E, K21M, T22P, T22A, T22V, T22D, T22R, T22K, T22M, T22E, T22H, T22L, T22W, T22F, T22C, T22I, G24Y, S25P, S27N, S27I, S27M, S27D, S27V, S27F, S27A, S27C, S27L, S27E, G28L, Y29W, S30K, S30D, S30H, S30T, D32Q, I38V, I38M, S39A, S39P, S39Y, S39H, S39E, S39N, S39M, S39D.Q40V, S42C, S42L, S42M, S42F, S42W, V49R, L51 I, K52I, K52H, A55S, D56I, D56L, D56T, S57W, S57F, S57H, S57C, S57P, S57V, S57R, S57T, Y58A, Y58T, S59C, S59T, S59L, S59Q, S59V, S59K, S59R, S59M, S59I, S59H, N61 D, P63A, T65L, T65I, T65V, T65R, T65K, S68V, S68I, S68W, S68Y, S68H, S68C, S68T, S68L, V76G, V76L, V76C, V76K, V76H, V76E, V76A, V76Y, V76N, V76M, V76R, V76F, T77N, T77Y, T77W, T77R, F78I, F78H, F78Y, F78C, T79G, T79R, N80K, S82L, S82E, S82K, S82R, S82H, D83C, D83F, D83L, L92T, A93G, E94N, G99S, S101D, S101A, S102M, S102L, S102V, S102A, S102K, S102T, S102R, T104P, T104A, T105V, T105I, K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, K107D, Q109R, Q109S, A112S, S116D, S116R, S116Q, S116H, S116V, S116A, S116E, S116K, A125K, S126I, S126E, S126A, S126C, T127C, T127V, S130E, G132R, D135R, T138Q, W139R, R143E, R143K, S144Q, S144H, S144L, S144P, S144E, S144K, G145V, G145E, G145D, G145A, A147Q, A147W, A147S, G149S, K152H, K152R, S156C, S156G, S156K, S156R, S156T, S156A, T157S, Y159F, K160V, W161 L, W161Y, G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, G162H, S164R, S164T, Q166D, S167M, S167L, S167F, S167W, S167E, S167A, S167Y, S167H, S167C, S167I, S167Q, S167V, S167T, S168V, S168E, S168D, S168L, K170S, K170L, K170F, K170R, T171 D, T171 E, T171A, T171C, A172G, A172S, L173T, L173A, L173V, Q174L, G175D, G175E, G175N, G175R, M176H, L177I, N178D, N178E, N178T, N178S, N178A, S179E, S181R, S181E, S181D, S181 F, S181H, S181W, S181 L, S181M, S181Y, S181Q, S181G, S181A, Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y182I, Y182E, Y182T and Y182W. The variant of this embodiment preferably comprises two or more of said substitutions, for example 2, 3, 4, 5, 6, 7, 8, 9, 10 or more of said substitutions.

[0068] In another embodiment, the variant of SEQ ID NO: 4 having DNase activity and having at least 80% sequence identity to SEQ ID NO: 5 is a variant comprising two or more substitutions selected from the group consisting of T1I, T1L, T1V, S13Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T127I, T127L, S144P, A147H, S167L, S167I, S167V, G175D and G175E. The variant of this embodiment preferably comprises two or more of said substitutions, for example 2, 3, 4,

[0069] 5, 6, 7, 8, 9, 10 or more of said substitutions.

[0070] In another embodiment, the variant of SEQ ID NO: 4 having DNase activity and having at least 80% sequence identity to SEQ ID NO: 5 is a variant comprising two or more substitutions selected from the group consisting of N61 D, T65I, T65V, S82R, K107Q, T127S, T127V, G149N and S164D, and preferably further comprising at least one substitution selected from the group consisting of Q14R, Q14W, K21 L, L33K, Q48D, D56I, D56L, S66Y, S68L, S102Y, S106A, Q109T, S116W, T171W, S181T and S181 W.

[0071] In other embodiments, the polypeptide having DNase activity may be the polypeptide of SEQ ID NO: 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16 or 17, or a variant thereof.

[0072] In one such embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 6 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 6.

[0073] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 7 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 7.

[0074] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 8 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 8.

[0075] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 9 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 9.

[0076] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 10 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 10. In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 11 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 11.

[0077] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 12 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 12.

[0078] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 13 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 13.

[0079] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 14 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 14.

[0080] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 15 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 15.

[0081] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 16 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 16.

[0082] In one embodiment, the polypeptide having DNase activity is the polypeptide of SEQ ID NO: 17 or a variant thereof, for example a polypeptide having at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 17. The soil release polymer used according to the invention preferably is a polyester and more preferably is a terephthalic acid-based polyester.

[0083] "Terephthalic acid-based polyester” in the context of the invention means a polyester that comprises one or more structural units of the formula (I)

[0084] (I).

[0085] Even more preferably, the soil release polymer used according to the invention is a polyester comprising one or more structural units of the formula (I) and one or more structural units of the formula (II) or mixtures thereof

[0086] -O(CnH2n)O- (II) wherein (CnH2n) is a linear or branched alkylene group with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (CsHe), and one or more terminal groups of the formula (lll-a) or mixtures thereof

[0087] (lll-a) wherein R1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 30 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 30 carbon atoms or mixtures thereof, preferably a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 20 carbon atoms or mixtures thereof, a is, based on a molar average, a number from 1 to 200, preferably from 10 to 200, more preferably from 10 to 150, and

[0088] R2is a linear or branched alkylene group (CmH2m) with m being a number from 2 to 10 or mixtures thereof, preferably with m being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (C2H4).

[0089] The one or more structural units of the formula (I) of the polyester used according to the invention are preferably derived from dialkyl esters of terephthalic acid or mixtures thereof, more preferably from (Ci-C4)-dialkyl esters of terephthalic acid or mixtures thereof and even more preferably from the dimethyl ester of terephthalic acid.

[0090] Preferably, the number of structural units of the formula (I) in the polyester used according to the invention is, based on a molar average, from 2 to 60, more preferably from 2 to 40, even more preferably from 2 to 30, particularly preferably from 2 to 20 and especially preferably from 5 to 20.

[0091] In addition to the one or more structural units of the formula (I) further structural units derived from other di- or polycarboxylic acids or their salts or their (di)alkylesters can be used in the polyester used according to the invention, such as, phthalic acid, isophthalic acid, 5-sulfoisophthalic acid, 3-sulfophthalic acid, 4-sulfophthalic acid, naphthalene-1 ,4-dicarboxylic acid, naphthalene-2,6-dicarboxylic acid, tetrahydrophthalic acid, trimellitic acid, diphenoxyethane-4,4'-dicarboxylic acid, diphenyl-4,4'-dicarboxylic acid, 2,5-furandicarboxylic acid, adipic acid, sebacic acid, decan-1 , 10-dicarboxylic acid, fumaric acid, succinic acid, 1 ,4-cyclohexanedicarboxylic acid, cyclohexanediacetic acid, glutaric acid, azelaic acid, or their salts or their (di)alkyl esters, preferably their (Ci-C4)-(di)alkyl esters and more preferably their (di)methyl esters, or mixtures thereof. Typically, such further structural units derived from other di- or polycarboxylic acids or their salts or their (di)alkylesters would be present to a minor extent, for example in an amount smaller than 5 wt.-%, based on the total weight of the polyester used according to the invention. The one or more structural units of the formula (II) of the polyesters used according to the invention are derived from glycol H-O(CnH2n)O-H, wherein “n” has the meaning given above.

[0092] Preferably, the one or more structural units of the formula (II) are derived from ethylene glycol, 1 ,2-propyleneglycol, 1 ,3-propyleneglycol, 2-methyl-1 ,3-propanediol, 1 ,4-butanediol, 1 ,3- butanediol, 2,3-butanediol, 1 ,2-butanediol, 2,2-dimethyl-1 ,3-propanediol, 1 ,2-pentanediol, 1 ,5-pentanediol, 1 ,2-hexanediol, 1 ,6-hexanediol or mixtures thereof.

[0093] In addition, further structural units derived from triols or tetraols can be used in the polyester used according to the invention, such as, glycerol, pentaerythritol or mixtures thereof. Typically, such further structural units would be present to a minor extent, for example in an amount smaller than 5 wt.-% based on the total weight of the polyester used according to the invention.

[0094] Examples of the alkyl and alkenyl groups R1in the one or more terminal groups of the formula (lll-a) are, for example, methyl, ethyl, vinyl, linear or branched propyl, butyl, pentyl, hexyl, heptyl, octyl (e.g. capryl), nonyl, decyl, undecyl, dodecyl, tridecyl (e.g. isotridecyl), tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, icosyl, henicosyl, docosyl, tricosyl, tetraicosyl, propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl, decenyl, dodecenyl, tetradecenyl, hexadecenyl, octadecenyl, octadecadienyl, octadecatrienyl, eicosenyl, eicosadienyl, eicosatetraenyl, docosenyl, docosahexaenyl, tetracosenyl, or mixtures thereof.

[0095] The groups R1in the one or more terminal groups of the formula (lll-a) may also be mixtures which have been derived or obtained from natural sources and comprise one or more alkyl and / or one or more alkenyl groups and in case these mixtures comprise one or more alkenyl groups, they may also be partially or totally hydrogenated. Examples of such mixtures are cocoyl, partially or totally hydrogenated variants of cocoyl, talloyl or partially or totally hydrogenated variants of talloyl.

[0096] In a preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 1 to 18 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 18 carbon atoms or mixtures thereof.

[0097] In a more preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 10 to 18 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 10 to 18 carbon atoms or mixtures thereof.

[0098] In an even more preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 10 to 16 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 10 to 16 carbon atoms or mixtures thereof.

[0099] In another preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 and preferably from 1 to 18 carbon atoms, or mixtures thereof.

[0100] In another more preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is methyl.

[0101] In another more preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 10 to 20 carbon atoms or mixtures thereof.

[0102] In another even more preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 10 to 18 carbon atoms or mixtures thereof.

[0103] In a particularly preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is a linear or branched, preferably a linear, alkyl group comprising from 10 to 16 carbon atoms or mixtures thereof.

[0104] In another preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is selected from the group consisting of linear or branched alkyl groups comprising from 10 to 16 carbon atoms (such as lauryl, myristyl or isotridecyl), cocoyl, partially or totally hydrogenated variants of cocoyl, talloyl, partially or totally hydrogenated variants of talloyl and mixtures thereof, more preferably is selected from the group consisting of linear or branched, preferably linear, alkyl groups comprising from 10 to 16 carbon atoms, talloyl, partially or totally hydrogenated variants of talloyl and mixtures thereof, even more preferably is selected from the group consisting of linear or branched, preferably linear, alkyl groups comprising from 10 to 16 carbon atoms, partially or totally hydrogenated variants of talloyl and mixtures thereof and particularly preferably is selected from the group consisting of lauryl, myristyl, partially or totally hydrogenated variants of talloyl and mixtures thereof.

[0105] In an extraordinarily preferred embodiment of the invention, R1in the one or more terminal groups of the formula (lll-a) is selected from the group consisting of lauryl, myristyl and mixtures thereof.

[0106] In the case that at least two different types of [0(CmH2m)] groups, for example [O(C2H4)], [□(CaHe)] and [O(C4H8)] groups, exist in a terminal group of the formula (lll-a), they may be arranged blockwise, alternating, periodically and / or statistically, preferably blockwise and / or statistically. This means that in a terminal group of the formula (lll-a), the [0(CmH2m)] groups, and e.g. the groups [O(C2H4)], [C CsHe)] and [O^Hs)], may be arranged, for example, in a purely statistically or blockwise form but may also be arranged in a form which could be considered as both statistical and blockwise, e.g. small blocks of [(OC2H4)] and [©(CsHe)] arranged in a statistical manner, or in a form wherein adjacent instances of statistical and blockwise arrangements of the groups [O(C2H4)], [C CsHe)] and [O(C4H8)] exist.

[0107] Any of the groups [0(CmH2m)], e.g. any of the groups [C C2H4)], [O(C3H6)] and [O(C4H8)J, can be linked to R1- and -O in a terminal group of the formula (lll-a). This means, for example, that both, R1- and -O in a terminal group of the formula (lll-a), may be connected to a [O(C2H4)] group, they may both be connected to a [O(C3H8)] group, they may both be connected to a [O(C4HS)] group or they may be connected to different groups selected from [O(C2H4)], [O(C3H6)] and [O(C4H8)].

[0108] In the case that one molecule of the polyesters used according to the invention comprises two or more terminal groups of the formula (lll-a) the definition of R1, R2and “a” may vary between those terminal groups.

[0109] In a more preferred embodiment of the invention, in the polyester used according to the invention, the number of structural units of the formula (I) is, based on a molar average, from 2 to 15, R1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 and preferably from 1 to 18 carbon atoms, or mixtures thereof, and a is, based on a molar average, a number from 10 to 150, preferably from 15 to 140.

[0110] In an even more preferred embodiment of the invention, in the polyester used according to the invention, the number of structural units of the formula (I) is, based on a molar average, from 2 to 15, R1is methyl, and a is, based on a molar average, a number from 10 to 100, preferably from 15 to 60. An example of such a polyester is TexCare® SRN 260.

[0111] In a further, even more preferred embodiment of the invention, in the polyester used according to the invention, the number of structural units of the formula (I) is, based on a molar average, from 5 to 15, R1is a linear or branched, preferably a linear, alkyl group comprising from 10 to 20 carbon atoms or mixtures thereof, and a is, based on a molar average, a number from 60 to 150, preferably from 70 to 140. In this further, even more preferred embodiment of the invention, R1is a linear or branched, preferably a linear, alkyl group comprising from 10 to 16 carbon atoms or mixtures thereof, and more preferably is selected from the group consisting of lauryl, myristyl and mixtures thereof.

[0112] In a preferred embodiment of the invention, the polyester used according to the invention comprises one or more polyalkyleneglycol-derived structural units of the formula (IV-a)

[0113] (IV-a) or mixtures thereof, wherein f is, based on a molar average, a number from 2 to 200, preferably from 10 to 200, more preferably from 20 to 150 and even more preferably from 20 to 120, and

[0114] R3is a linear or branched alkylene group (CnH2n) with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (C2H4).

[0115] In the case that at least two different types of [(CnH2n)O] groups, for example [(C2H4)O], [(CsHe)®] and [(C4Hs)O] groups, exist in a structural unit of the formula (IV-a), they may be arranged blockwise, alternating, periodically and / or statistically, preferably blockwise and / or statistically. This means that in a structural unit of the formula (IV-a), the [(CnH2n)O] groups, and e.g. the groups [(C2H4)O], [(CaHejO] and [(C4Hs)O], may be arranged, for example, in a purely statistically or blockwise form but may also be arranged in a form which could be considered as both statistical and blockwise, e.g. small blocks of [(C2H4)O] and [(CaHejO] arranged in a statistical manner, or in a form wherein adjacent instances of statistical and blockwise arrangements of the groups [(C2H4)O], [(CsHejO] and [(C4Hs)O] exist. Within the structure element -[R3-O]f- in a structural unit of the formula (IV-a) any of the groups [(CnH2n)O], e.g. any of the groups [(C2H4)O], [(CsHejO] and [(C4Hs)O], can form an end group of the structure element -[R3-0]r. This means, for example, that the two end groups of the structure element -[R3-O]f- in a structural unit of the formula (IV-a) may be formed by [(C2H4)O] groups, may be formed by [(CsHejO] groups, may be formed by [(C4Hs)O] groups or may be formed by different groups selected from [(C2H4)O], [(CsHejO] and [(C4Hs)O].

[0116] In the case that one molecule of the polyesters used according to the invention comprises two or more structural units of the formula (IV-a) the definition of R3and “f” may vary between those structural units.

[0117] In the polyester used according to the invention, the amount of the one or more terminal groups of the formula (lll-a) or mixtures thereof preferably is at least 40 wt.-%, more preferably is from 40 to 90 wt.-% and even more preferably is from 50 to 90 wt.-%, in each case based on the total weight of the polyester.

[0118] In the polyester used according to the invention, the combined amount of the one or more structural units of the formula (I), and the one or more structural units of the formula (II) or mixtures thereof, and the one or more terminal groups of the formula (lll-a) or mixtures thereof, and, if present, the one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof, preferably is at least 50 wt.-%, more preferably is at least 60 wt.-% and even more preferably is at least 70 wt.-%, in each case based on the total weight of the polyester.

[0119] The weight average molecular weight (Mw) of the soil release polymer or of the polyester used according to the invention preferably is from 1500 to 20000 g / mol and more preferably from 5000 to 20000 g / mol.

[0120] The weight average molecular weight (Mw) of the soil release polymer or of the polyester used according to the invention may be determined by GPC analysis, preferably as detailed in the following: 10 pl of sample is injected onto a PSS Suprema column of dimensions 300 x 8 mm with porosity 30 A and particle size 10 pm. The detection is monitored at 235 nm on a multiple wavelength detector. The employed eluent is 1 .25 g / l of disodium hydrogen phosphate in a 45 / 55 % (v / v) water I acetonitrile mixture. Separations are conducted at a flow rate of 0.8 ml / minute. Quantification is performed by externally calibrating standard samples of different molecular weight polyethylene glycols. The polyester used according to the invention comprises one or more terminal groups of the formula (lll-a) or mixtures thereof. In addition to these one or more terminal groups or mixtures thereof, the polyester used according to the invention may comprise further terminal groups, preferably selected from the group consisting of -OH, -O(C2H2n)OH, wherein “n” has the meaning given above, and mixtures thereof. In case the polyester used according to the invention comprises one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof, the polyester may also comprise terminal groups of the formula -O-[R3-O]f-H wherein R3and “f” have the meaning given above.

[0121] Preferably, the polyester molecules of the polyester used according to the invention comprise two or more terminal groups of the formula (lll-a) or mixtures thereof. Even more preferably, all terminal groups of the polyester molecules of the polyester used according to the invention are terminal groups of the formula (lll-a) or mixtures thereof.

[0122] In a preferred embodiment of the invention, the polyester used according to the invention consists of a1) one or more structural units of the formula (I) and a2) one or more structural units of the formula (II) or mixtures thereof and a3) one or more terminal groups of the formula (lll-a) or mixtures thereof.

[0123] In a further preferred embodiment of the invention, the polyester used according to the invention, consists of b1) one or more structural units of the formula (I) and b2) one or more structural units of the formula (II) or mixtures thereof and b3) one or more terminal groups of the formula (lll-a) or mixtures thereof, and b4) one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof.

[0124] The soil release polymer or the polyester used according to the invention preferably is anionic or nonionic and more preferably is nonionic.

[0125] The groups (C2H4) in the structural units of the formula (II), in the terminal groups of the formula (lll-a) or in the structural units of the formula (IV-a) preferably are of the formula -CH2-CH2-. The groups (CsHe) in the structural units of the formula (II), in the terminal groups of the formula (lll-a) or in the structural units of the formula (IV-a) preferably are of the formula -CH(CH3)-CH2- or -CH2-CH(CH3)-, i.e. of the formula:

[0126] The groups (C4H8) in the structural units of the formula (II), in the terminal groups of the formula (lll-a) or in the structural units of the formula (IV-a) preferably are of the formula -CH(CH3)- CH(CH3)-, i.e. of the formula:

[0127] C H3CH3

[0128] - C H — C H -

[0129] In the soil release polyester used according to the invention and the terephthalic acid-based soil release polyesters contained in the laundry detergent composition of the invention, the terminal groups or structural units of the formulae (II), (lll-a) or (IV-a) may be linked directly to structural units of the formula (I) resulting in ester groups.

[0130] The following paragraphs will show illustrative, but by no means limiting, structural entities that can be found in the soil release polyester used according to the invention and the terephthalic acid-based soil release polyesters contained in the laundry detergent composition of the invention.

[0131] The structural units of the formula (I) and optional additional di- or polycarboxylic acid-derived structural units are linked indirectly, preferably via the structural units of the formula (II), which - in the case of structural units of the formulae (I) and (II), wherein the structural units of the formula (II) are derived from 1 ,2-propylene glycol - results in the following structural entity: Preferably, the terminal group of the formula (lll-a) is linked to an acyl group derived from a dicarboxylic acid, preferably to the structural unit of the formula (I), which - in the case of structural unit of the formula (I) and terminal group of the formula (lll-a) - results in the following structural entity:

[0132] In the use according to the invention, the textile preferably comprises or consists essentially of polyester or another synthetic material and more preferably comprises or consists essentially of polyester.

[0133] In the use according to the invention, the polypeptide having DNase activity and the soil release polymer or the polyester preferably are present in a laundry detergent composition.

[0134] A further subject matter of the present invention is laundry detergent compositions comprising Z1) one or more polypeptides having DNase activity and having at least 80% sequence identity to SEQ ID NO: 1, 2 or 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, and

[0135] Z2) one or more terephthalic acid-based soil release polymers.

[0136] Preferably, the one or more polypeptides having DNase activity with at least 80% sequence identity to SEQ ID NO: 1, 2 or 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, of component Z1) of the inventive laundry detergent compositions are those polypeptides that have been described above in connection with the use according to the invention.

[0137] The amount of one or more polypeptides having DNase activity in the detergent composition may vary depending on factors such as the degree of concentration or compactness of the composition and the desired DNase concentration in the wash liquor. The DNase will normally be included in the detergent composition in an amount of up to about 10,000 ppm, typically up to about 5000 ppm or up to about 2000 ppm, based on the total weight of the laundry detergent composition. The DNase can e.g. be included in the composition at a level of from 1 ppm to 10,000 ppm, such as from 10 ppm to 5000 ppm, from 20 ppm to 2000 ppm, from 50 ppm to 1000 ppm, from 80 ppm to 600 ppm, or from 100 ppm to 500 ppm, based on the total weight of the laundry detergent composition. The concentration of the polypeptide having DNase activity in the wash liquor is typically in the range of from 0.0001 mg / l to 10 mg / l enzyme protein, from 0.0002 mg / l to 10 mg / l, from 0.001 mg / l to 10 mg / l, from 0.002 mg / l to 10 mg / l, from 0.01 mg / l to 10 mg / l, from 0.02 mg / l to 10 mg / l, from 0.1 mg / l to 10 mg / l, from 0.2 mg / l to 10 mg / l, or from 0.2 mg / l to 5 mg / l.

[0138] The concentration of the soil release polymer in the wash liquor is typically in the range of from 0.001 g / l to 0.5 g / l, from 0.002 g / l to 0.5 g / l, from 0.01 g / l to 0.5 g / l, from 0.02 g / l to 0.5 g / l, from 0.1 g / l to 0.5 g / l, or from 0.2 g / l to 0.4 g / l, or from 0.001 g / l to 0.2 g / l, from 0.002 g / l to 0.2 g / l, from 0.01 g / l to 0.2 g / l, from 0.02 g / l to 0.2 g / l, or from 0.1 g / l to 0.2 g / l.

[0139] Preferably, the one or more terephthalic acid-based soil release polymers of component Z2) of the inventive laundry detergent composition are terephthalic acid-based polyesters and more preferably are those terephthalic acid-based polyesters that have been described above in connection with the use according to the invention.

[0140] The laundry detergent compositions of the invention comprise the one or more terephthalic acid-based soil release polymers of component Z2) preferably in an amount of at least 0.1 wt.- %, more preferably in an amount from 0.1 to 10 wt.-%, even more preferably in an amount from 0.2 to 5 wt.-% and particularly preferably in an amount from 0.2 to 3 wt.-%, in each case based on the total weight of the laundry detergent composition.

[0141] The soil release polymers used according to the invention and the terephthalic acid-based soil release polymers contained in the laundry detergent compositions of the invention may be prepared by methods known to the person skilled in the art.

[0142] It is to be understood that the soil release polymer, and in particular the polyester, used according to the invention or the terephthalic acid-based soil release polymers, and in particular the terephthalic acid-based polyesters, used according to the invention or contained in the laundry detergent composition of the invention are typically prepared by polycondensation processes. This leads to statistically determined mixtures of polymers or polyesters in which a mixture of molecular species with a distribution around a molar average is obtained.

[0143] For the preparation of the polyesters used according to the invention, and in particular for the preparation of the terephthalic acid-based polyesters used according to the invention or contained in the laundry detergent compositions of the invention, e.g. a two-stage process of transesterification of diesters and diols, followed by a polycondensation reaction under reduced pressure, can be used. For example, the process for the preparation of the terephthalic acid-based polyesters used according to the invention or contained in the laundry detergent compositions of the invention comprises the steps of: heating one or more substances of the formula Q1-OOC-CeH4-COO-Q2, wherein Q1 and Q2, independently of one another, are e.g. selected from (Ci-C4)-alkyl and preferably are CH3, and one or more glycols H-O(CnH2n)O-H or mixtures thereof, wherein “n” has the meaning given above, and one or more substances of the formula R1-[O-R2]a-OH or mixtures thereof, wherein R1, R2and “a” have the meaning given above, with the addition of a catalyst, to temperatures of from 160 to 220°C, preferably beginning at atmospheric pressure, and then continuing the reaction under reduced pressure at temperatures of from 160 to 240°C.

[0144] Reduced pressure preferably means a pressure of from 0.1 to 900 mbar and more preferably a pressure of from 0.5 to 500 mbar.

[0145] Typical transesterification and condensation catalysts known in the art can be used for the process for the preparation of the polyesters used according to the invention or contained in the laundry detergent compositions of the invention, such as antimony, germanium and titanium- based catalysts. Preferably, tetraisopropyl orthotitanate (I PT) and sodium acetate (NaOAc) are used as the catalyst system in the process for the preparation of the polyesters used according to the invention or contained in the laundry detergent compositions of the invention.

[0146] In order to obtain polyesters comprising one or more polyalkyleneglycol-derived structural units of the formula -O-[R3-O]f- (IV-a) or mixtures thereof, the respective one or more polyalkyleneglycols of the formula HO-[R3-O]f-H or mixtures thereof, may be employed in the process for the preparation of the polyesters used according to the invention or contained in the laundry detergent compositions of the invention.

[0147] The preparation of soil release polyesters is also described in e.g. WO 2019 / 224030 A1 or WO 2021 / 233987 A1. Soil release polymers are also commercially available.

[0148] The laundry detergent compositions of the invention preferably comprise Z3) one or more surfactants.

[0149] Surfactants assist in removing soil from textile materials and also assist in maintaining removed soil in solution or suspension in the wash liquor. Preferably, the one or more surfactants of component Z3) of the laundry detergent compositions of the invention are selected from the group consisting of anionic, nonionic, cationic and zwitterionic surfactants, and more preferably from the group consisting of anionic, nonionic and zwitterionic surfactants.

[0150] Anionic Surfactants

[0151] Suitable anionic surfactants that may be used are any of the conventional anionic surfactant types typically used in laundry detergent compositions. These include alkyl sulfonates, alkyl ether sulfates, alkyl sulfates, alkyl ester sulfonates and soaps. Preferred anionic surfactants are alkylbenzene sulfonates, alkyl ether sulfates, alkyl sulfates and soaps.

[0152] Preferred alkyl sulfonates are alkylbenzene sulfonates, particularly linear alkylbenzene sulfonates (LAS) having an alkyl chain length of Cs-Cis. Possible counter ions for concentrated alkaline liquids are ammonium ions, e.g. those generated by the neutralization of alkylbenzene sulfonic acid with one or more ethanolamines, for example monoethanolamine (MEA) and triethanolamine (TEA), or alternatively, alkali metals, e.g. those arising from the neutralization of alkylbenzene sulfonic acid with alkali hydroxides. The linear alkyl benzene sulfonate surfactants may be LAS with an alkyl chain length of preferably from 8 to 15 and more preferably from 12 to 14. The neutralization of the acid may be performed before addition to the laundry detergent compositions of the invention or during the process of formulating the laundry detergent compositions of the invention through excess addition of neutralizing agent.

[0153] Preferred alkyl ether sulfates (AES) are alkyl polyethoxylate sulfate anionic surfactants of the formula

[0154] R4O(C2H4O)ySO3 M+wherein

[0155] R4is a saturated or unsaturated alkyl chain having preferably from 10 to 22 carbon atoms, and more preferably from 12 to 16 carbon atoms,

[0156] M+is a cation which makes the compound water-soluble, preferably an ammonium cation, a substituted ammonium cation, an alkali metal cation, or other material chosen from the list of buffers, and y averages preferably from 1 to 15, more preferably from 1 to 3 and even more preferably is 3.

[0157] Preferred alkyl sulfates (AS) are surfactants of the formula R5OSO3’M wherein

[0158] R5is a linear or branched alkyl chain having preferably from 8 to 24 carbon atoms, and more preferably from 12 to 18 carbon atoms, and

[0159] M+is a cation which makes the compound water-soluble, preferably an ammonium cation, a substituted ammonium cation, an alkali metal cation, or other material chosen from the list of buffers.

[0160] Soaps are preferably fatty acids and more preferably linear saturated or unsaturated fatty acids having from 10 to 18 carbon atoms.

[0161] Nonionic Surfactants

[0162] Nonionic surfactants include primary and secondary alcohol ethoxylates, especially C8-C20 aliphatic alcohol ethoxylated with an average of from 1 to 35 moles of ethylene oxide per mole of alcohol, and more especially the C10-C15 primary and secondary aliphatic alcohols ethoxylated with an average of from 1 to 10 moles of ethylene oxide per mole of alcohol. Nonethoxylated nonionic surfactants include alkyl polyglycosides, glycerol monoethers and polyhydroxy amides (glucamides) such as N-methyl glucamides. Mixtures of nonionic surfactant may be used.

[0163] If included therein, the laundry detergent compositions of the invention contain preferably from 0.2 to 40 wt.-% and more preferably from 1 to 20 wt.-% of a nonionic surfactant, such as alcohol ethoxylate, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamineoxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, polyhydroxy alkyl fatty acid amide, or N-acyl N-alkyl derivatives of glucosamine ("glucamides"), in each case based on the total weight of the laundry detergent composition.

[0164] Zwitterionic Surfactants

[0165] The laundry detergent compositions of the invention may comprise up to 10 wt.-% of a zwitterionic surfactant, e.g. amine oxide or betaine, based on the total weight of the laundry detergent composition.

[0166] Typical amine oxides used are of the formula

[0167] R6N(O)(CH2R7)2 wherein

[0168] R6is a long chain moiety and each CH27are short chain moieties, and

[0169] R7is preferably selected from the group consisting of H, CH3 and -CH2OH.

[0170] In general, R6is a primary or branched hydrocarbyl moiety with a chain length of from 8 to 18, which can be saturated or unsaturated. Preferably, R6is a primary alkyl moiety with a chain length of 8 to 18 carbon atoms.

[0171] Preferred amine oxides have compositions wherein R6is a Cs-Cis alkyl and R7is H. These amine oxides are illustrated by C12-14 alkyldimethyl amine oxide, hexadecyl dimethylamine oxide, octadecylamine oxide.

[0172] A preferred amine oxide material is lauryl dimethylamine oxide, also known as dodecyldimethylamine oxide or DDAO.

[0173] Betaines may be alkyldimethyl betaines or alkylamido betaines, wherein the alkyl groups have C12-18 chains.

[0174] In a preferred embodiment of the invention, the one or more surfactants of component Z3) of the laundry detergent compositions of the invention are selected from the group consisting of linear alkyl benzene sulfonates, alkyl ether sulfates, alkyl sulfates, soaps, nonionic surfactants, amine oxides and betaines, and preferably the one or more surfactants of component Z3) of the laundry detergent compositions of the invention are selected from the group consisting of linear alkyl benzene sulfonates, alkyl ether sulfates, alkyl sulfates, soaps and nonionic surfactants.

[0175] Additional Surfactants

[0176] Other surfactants than the preferred LAS, AES, AS, soaps and nonionic surfactants may be added to the mixture of surfactants.

[0177] The laundry detergent compositions of the invention comprise the one or more surfactants of component Z3) preferably in an amount of at least 3 wt.-%, more preferably in an amount from 3 to 65 wt.-%, even more preferably in an amount from 4 to 60 wt.-% and particularly preferably in an amount from 5 to 55 wt.-%, in each case based on the total weight of the laundry detergent composition. Further Optional Ingredients

[0178] In addition to the one or more polypeptides having DNase activity, for example polypeptides having at least 80% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, of component Z1), and the one or more terephthalic acid-based soil release polymers of component Z2) and optionally the one or more surfactants of component Z3), the laundry detergent compositions of the invention may comprise one or more further optional ingredients, e.g. they may comprise conventional ingredients commonly used in laundry detergent compositions. Examples of optional ingredients include, but are not limited to builders, bleaching agents, bleach active compounds, bleach activators, bleach catalysts, photobleaches, dye transfer inhibitors, colour protection agents, anti-redeposition agents, dispersing agents, fabric softening and antistatic agents, fluorescent whitening agents, further enzymes, enzyme stabilizing agents, foam regulators, defoamers, malodor reducers, preservatives, disinfecting agents, hydrotropes, fibre lubricants, antishrinkage agents, buffers, fragrances, processing aids, colorants, dyes, pigments, anti-corrosion agents, fillers, stabilizers and other conventional ingredients for laundry detergent compositions.

[0179] Polyalkoxylated polyethyleneimine

[0180] For detergency boosting, it is advantageous to use a second polymer alongside the one or more terephthalic acid-based soil release polymers of component Z2) in the laundry detergent compositions of the invention. This second polymer is preferably a polyalkoxylated polyethyleneimine (EPEI). Polyethylene imines are materials composed of ethylene imine units -CH2CH2NH- and, where branched, the hydrogen on the nitrogen is replaced by another chain of ethylene imine units. These polyethyleneimines can be prepared, for example, by polymerizing ethyleneimine in the presence of a catalyst such as carbon dioxide, sodium bisulfite, sulfuric acid, hydrogen peroxide, hydrochloric acid, acetic acid, and the like.

[0181] Other Polymers

[0182] In addition to the one or more terephthalic acid-based soil release polymers of component Z2) and the optional EPEI, the laundry detergent compositions of the invention may comprise other polymeric materials, for example: dye transfer inhibition polymers, anti-redeposition polymers and cotton soil release polymers, especially those based on modified cellulosic materials. Especially, if EPEI is not present, the laundry detergent compositions of the invention may further comprise a polymer of polyethylene glycol and vinyl acetate, for example the lightly grafted copolymers described in WO 2007 / 138054. Such amphiphilic graft polymers based on water soluble polyalkylene oxides as graft base and side chains formed by polymerisation of a vinyl ester component have the ability to enable reduction of surfactant levels whilst maintaining high levels of oily soil removal. Hydrotropes

[0183] In the context of this invention a hydrotrope is a solvent that is neither water nor conventional surfactant that aids the solubilisation of the surfactants and other components, especially polymer and sequestrant, in the liquid to render it isotropic. Among suitable hydrotropes there may be mentioned as preferred: monopropylene glycol (MPG), glycerol, sodium cumene sulfonate, ethanol, other glycols, e.g. dipropylene glycol, diethers and urea. MPG and glycerol are preferred hydrotropes.

[0184] Further Enzymes

[0185] It is preferable that one or more further enzymes are present in the laundry detergent compositions of the invention. Preferably, the compositions comprise one or more further enzymes selected from protease, mannanase, pectate lyase, cutinase, lipase, amylase and cellulase. Other further enzymes may be selected from hexosaminidase, esterase, peroxidase and oxidase. The further enzymes are preferably present together with one or more corresponding enzyme stabilizers. The total further enzyme content in the laundry detergent compositions of the invention is preferably from 0 to 5 wt.-%, more preferably from 0.2 to 4 wt.- % and even more preferably from 0.4 to 2 wt.-%, in each case based on the total weight of the laundry detergent composition.

[0186] Sequestrants

[0187] Sequestrants are preferably included. Preferred sequestrants include organic phosphonates, alkanehydroxy phosphonates and carboxylates available under the DEQUEST trademark from Thermphos. The preferred sequestrant level is less than 10 wt.-% and preferably less than 5 wt.-%, in each case based on the total weight of the laundry detergent composition of the invention. A particularly preferred sequestrant is HEDP (1-Hydroxyethylidene-1,1-diphosphonic acid). Also suitable but less preferred as it gives inferior cleaning results is diethylenetriamine penta(methylene phosphonic acid) (DTPMP) or Heptasodium DTPMP.

[0188] Buffers

[0189] In addition to agents optionally included for the generation of anionic surfactants, e.g. from LAS or fatty acids, the presence of buffer is preferred for pH control. Possible buffers are one or more ethanolamines, e.g. monoethanolamine (MEA) or triethanolamine (TEA). They are preferably used in the laundry detergent compositions of the invention at levels of from 1 to 15 wt.-%, based on the total weight of the laundry detergent composition. Other suitable amino alcohol buffer materials may be selected from the group consisting of compounds having a molecular weight above 61 g / mol, which includes MEA. Suitable materials also include, in addition to the already mentioned materials: monoisopropanolamine, diisopropanolamine, triisopropanolamine, monoamino hexanol, 2-[(2-methoxyethyl) methylamino]-ethanol, propanolamine, N-methylethanolamine, diethanolamine, monobutanolamine, isobutanolamine, monopentanolamine, 1-amino-3-(2-methoxyethoxy)-2-propanol, 2-methyl-4-(methylamino)-2- butanol and mixtures thereof.

[0190] Potential alternatives to amino ethanol buffers are alkali hydroxides such as sodium hydroxide or potassium hydroxide.

[0191] Builders

[0192] Further washing and cleaning ingredients which may be present in the laundry detergent compositions of the invention include inorganic and / or organic builders in order to reduce the degree of hardness of the water. These builders may be present in the laundry detergent compositions of the invention in amounts of from about 5 to about 80 wt.-%, based on the total weight of the laundry detergent compositions. Inorganic builders include, for example, alkali metal, ammonium and alkanolammonium salts of polyphosphates, silicates, carbonates, sulfates and aluminosilicates.

[0193] Suitable organic builders include polycarboxyl compounds, such as, for example, ether polycarboxylates, ether hydroxypolycarboxylates, copolymers of maleic anhydride with ethylene or vinyl methyl ether, 1,3,5-trihydroxybenzene-2,4,6-trisulfonic acid and carboxymethyloxysuccinic acid, the alkali metal, ammonium and substituted ammonium salts of polyacetic acids, such as, for example, ethylenediaminetetraacetic acid and nitrilotriacetic acid, and also polycarboxylic acids, such as mellitic acid, succinic acid, oxydisuccinic acid, polymaleic acid, benzene-1 ,3,5-tricarboxylic acid, carboxymethyloxysuccinic acid, and soluble salts thereof. Builders based on citrates, for example citric acid and its soluble salts, in particular the sodium salt, are preferred polycarboxylic acid builders, which can also be used in granulated compositions, in particular together with zeolites and / or sheet silicates.

[0194] It may be advantageous to include fluorescer and / or bleach catalyst in the laundry detergent compositions of the invention as further high efficiency performance additives. Perfume and colorants will also desirably be included. The laundry detergent compositions of the invention may additionally contain viscosity modifiers, foam boosting agents, preservatives (e.g. bactericides), pH buffering agents, polyelectrolytes, anti-shrinking agents, anti-wrinkle agents, anti-oxidants, sunscreens, anti-corrosion agents, drape imparting agents, anti-static agents and ironing aids. The laundry detergent compositions of the invention may further comprise pearlisers and / or opacifiers or other visual cues and shading dye. Form, packaging and dosing

[0195] The laundry detergent compositions of the invention may be in solid or in liguid form, including a gel form. The laundry detergent compositions of the invention may be packaged as unit doses in a polymeric film soluble in the wash water. Alternatively, the laundry detergent compositions of the invention may be supplied in multidose plastics packs with a top or bottom closure. A dosing measure may be supplied with the pack either as a part of the cap or as an integrated system.

[0196] In one embodiment of the invention the laundry detergent composition is solid.

[0197] In a further embodiment of the invention the laundry detergent composition is liguid.

[0198] Preferred embodiments

[0199] Preferred embodiments of the invention are set forth in the following paragraphs:

[0200] 1. Use of a polypeptide having DNase activity and a soil release polymer in a laundry process for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile.

[0201] 2. The use according to paragraph 1, characterized in that the polypeptide having DNase activity has at least 80% seguence identity to SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16 or SEQ ID NO: 17, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% seguence identity to SEQ ID NO: 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or 17.

[0202] 3. The use according to paragraph 2, characterized in that the polypeptide having DNase activity comprises the amino acid seguence of SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3, or is a variant of SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3, wherein the variant has at least 80% but less than 100% seguence identity to SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% or at least 95% seguence identity to SEQ ID NO: 1 , 2 or 3; and preferably wherein said variant comprises one or more alterations selected from the group consisting of: a) one or more substitutions selected from the group consisting of N4E, L17E, T19A, T19G, T19I, K36P, Q38P, S39V, S39R, A40P, A40H, L41T, L41 H, V45H, L51G, K53T, K53P, G54P, A55P, N57H, E64A, E64Q, E64R, E64T, E64I, E64S, T66H, K67A, K67T, N68V, N68P, N68I, N68H, S69A, S69D, S69E, S69K, S69L, S69W, S69Y, S69Q, N70T, N70H, N70G, R71T, D72E, S74H, S74G, G75I, N77T, K82P, K82I, D83T, D83P, D83I, D83H, D83G, P84H, Q85T, Q85P, Q85H, K86T, K86P, K86H, G88P, G88H, A91P, W99T, A101W, K105E, K105N, K105T, K105D, S106T, S115T, L116I, Q135L, G136L, V138I, V138L, V138P, V138Q, L139A, N140R, N140L, N140A, G141L, F151 R, D152Y, D152L, D152I, D152A, P153E, S154R, T162R, W163E, F164R, I166Y, I166R, K168N, F169R, F169E, A173I, A173R, A173T, S182R, N183E, D184I, K185Y, S186I, D189G, D189H, K212G, K212P and K215I; b) two or more alterations selected from the group consisting of S69V, Q102E, S26*, D32Q, K36C,H, G37R, F43W, D46G, A55I, N68D, A76I, K82S,T, P84D.T, K86G,L,N,Q,T,V,Y, A91R, L92E, K95I, P97E.N, A101 E, K105N,G,Q,T,D, F112Y.W, L129K, N133Q, V138C, N140H, G141Q.R, S144E, N146A, K147N.E, V148I, A149D,E,F, Q150D, P153D.V, S154E, K155E,F,L,S,T, Q157D.E, Q158D, T159Q, K160D, T170Q, A172D,E,H,R, K185*, V187N.Y, N191*, K192A, D197K.S, G199Q, Q208V, E211Y,T,P, N213S, N214D, N217A and Y218D.E; c) at least two substitutions, such as at least three substitutions, selected from the group consisting of A111 P, D32E, V35I, S69V, Q102E, K105N and G181 N; and d) the substitution A111 P and two or more substitutions selected from the group consisting of S26H, D32E, V35I, K65E, K67A, S69E, S69V, S98R, Q102E, K105N, S115T, Q150E, Q157E, T159Q, G161R, A172E, G181 N, S182Y, S182V, K185E, V187N, K192I, A206G, Q208V and K212E, wherein position numbers are based on the numbering of SEQ ID NO: 1.

[0203] 4. The use according to paragraph 2, characterized in that the polypeptide having DNase activity has at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4.

[0204] 5. The use according to paragraph 2, characterized in that the polypeptide having DNase activity is a variant of SEQ ID NO: 4, wherein the variant has at least 80% sequence identity to SEQ ID NO: 5, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 5; and preferably wherein said variant comprises one or more substitutions selected from the group consisting of: a) one or more substitutions selected from the group consisting of T11, T1V, T1Y, T1M, T1 E, G4N, T5F, T5C, P6V, P6G, S7D, S7T, K8V, S9K, S9Q, S9V, S9L, S9F, S9P, S9R, A10D, A10M, A10I, A10Q, A10V, A10L, A10K, Q12S, Q12V, Q12E, S13D, S13Y, S13Q, S13F, S13R, S13V, S13N, S13H, S13M, S13W, S13K, S13L, S13E, Q14M, Q14R, N16S, A17C, A17V, A17E, A17T, T19K, T19L, T19S, T19I, T19V, K21 E, K21M, T22P, T22A, T22V, T22D, T22R, T22K, T22M, T22E, T22H, T22L, T22W, T22F, T22C, T22I, G24Y, S25P, S27N, S27I, S27M, S27D, S27V, S27F, S27A, S27C, S27L, S27E, G28L, Y29W, S30K, S30D, S30H, S30T, D32Q, I38V, I38M, S39A, S39P, S39Y, S39H, S39E, S39N, S39M, S39D.Q40V, S42C, S42L, S42M, S42F, S42W, V49R, L51 I, K52I, K52H, A55S, D56I, D56L, D56T, S57W, S57F, S57H, S57C, S57P, S57V, S57R, S57T, Y58A, Y58T, S59C, S59T, S59L, S59Q, S59V, S59K, S59R, S59M, S59I, S59H, N61 D, P63A, T65L, T65I, T65V, T65R, T65K, S68V, S68I, S68W, S68Y, S68H, S68C, S68T, S68L, V76G, V76L, V76C, V76K, V76H, V76E, V76A, V76Y, V76N, V76M, V76R, V76F, T77N, T77Y, T77W, T77R, F78I, F78H, F78Y, F78C, T79G, T79R, N80K, S82L, S82E, S82K, S82R, S82H, D83C, D83F, D83L, L92T, A93G, E94N, G99S, S101D, S101A, S102M, S102L, S102V, S102A, S102K, S102T, S102R, T104P, T104A, T105V, T105I, K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, K107D, Q109R, Q109S, A112S, S116D, S116R, S116Q, S116H, S116V, S116A, S116E, S116K, A125K, S126I, S126E, S126A, S126C, T127C, T127V, S130E, G132R, D135R, T138Q, W139R, R143E, R143K, S144Q, S144H, S144L, S144P, S144E, S144K, G145V, G145E, G145D, G145A, A147Q, A147W, A147S, G149S, K152H, K152R, S156C, S156G, S156K, S156R, S156T, S156A, T157S, Y159F, K160V, W161L, W161Y, G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, G162H, S164R, S164T, Q166D, S167M, S167L, S167F, S167W, S167E, S167A, S167Y, S167H, S167C, S167I, S167Q, S167V, S167T, S168V, S168E, S168D, S168L, K170S, K170L, K170F, K170R, T171 D, T171 E, T171A, T171C, A172G, A172S, L173T, L173A, L173V, Q174L, G175D, G175E, G175N, G175R, M176H, L177I, N178D, N178E, N178T, N178S, N178A, S179E, S181R, S181E, S181D, S181 F, S181H, S181W, S181 L, S181M, S181Y, S181Q, S181G, S181A, Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y182I, Y182E, Y182T and Y182W; b) two or more substitutions selected from the group consisting of T1 I, T1L, T1V, S13Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T127I, T127L, S144P, A147H, S167L, S167I, S167V, G175D and G175E; and c) two or more substitutions selected from the group consisting of N61 D, T65I, T65V, S82R, K107Q, T127S, T127V, G149N and S164D, and preferably at least one substitution selected from the group consisting of Q14R, Q14W, K21 L, L33K, Q48D, D56I, D56L, S66Y, S68L, S102Y, S106A, Q109T, S116W, T171W, S181T and S181 W; wherein position numbers are based on the numbering of SEQ ID NO: 4.

[0205] 6. The use according to one or more of paragraphs 1 to 5, characterized in that the soil release polymer is a polyester comprising one or more structural units of the formula (I) and one or more structural units of the formula (II) or mixtures thereof

[0206] -O(CnH2n)O- (II) wherein (CnH2n) is a linear or branched alkylene group with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (CsHe), and one or more terminal groups of the formula (lll-a) or mixtures thereof

[0207] (lll-a) wherein

[0208] R1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 30 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 30 carbon atoms or mixtures thereof, preferably a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 20 carbon atoms or mixtures thereof, a is, based on a molar average, a number from 1 to 200, preferably from 10 to 200, more preferably from 10 to 150, and

[0209] R2is a linear or branched alkylene group (CmH2m) with m being a number from 2 to 10 or mixtures thereof, preferably with m being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (C2H4).

[0210] 7. The use according to paragraph 6, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 2 to 60, preferably from 2 to 40, more preferably from 2 to 30, even more preferably from 2 to 20 and particularly preferably from 5 to 20.

[0211] 8. The use according to paragraph 6 or 7, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 2 to 15, R1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 and preferably from 1 to 18 carbon atoms, or mixtures thereof, and a is, based on a molar average, a number from 10 to 150, preferably from 15 to 140.

[0212] 9. The use according to one or more of paragraphs 6 to 8, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 2 to 15, R1is methyl, and a is, based on a molar average, a number from 10 to 100, preferably from 15 to 60. An example of such a polyester is TexCare® SRN 260.

[0213] 10. The use according to one or more of paragraphs 6 to 8, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 5 to 15, R1is a linear or branched, preferably a linear, alkyl group comprising from 10 to 20 carbon atoms or mixtures thereof, and a is, based on a molar average, a number from 60 to 150, preferably from 70 to 140.

[0214] 11 . The use according to paragraph 10, characterized in that R1in the polyester is a linear or branched, preferably a linear, alkyl group comprising from 10 to 16 carbon atoms or mixtures thereof, and even more preferably is selected from the group consisting of lauryl, myristyl and mixtures thereof.

[0215] 12. The use according to one or more of paragraphs 6 to 11 , characterized in that the polyester comprises one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof, wherein f is, based on a molar average, a number from 2 to 200, preferably from 10 to 200, more preferably from 20 to 150 and even more preferably from 20 to 120, and

[0216] R3is a linear or branched alkylene group (CnH2n), with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (C2H4).

[0217] 13. The use according to one or more of paragraphs 6 to 12, characterized in that the amount of the one or more terminal groups of the formula (lll-a) or mixtures thereof in the polyester is at least 40 wt.-%, preferably is from 40 to 90 wt.-% and more preferably is from 50 to 90 wt.-%, in each case based on the total weight of the polyester.

[0218] 14. The use according to one or more of paragraphs 6 to 13, characterized in that the combined amount of the one or more structural units of the formula (I), and the one or more structural units of the formula (II) or mixtures thereof, and the one or more terminal groups of the formula (lll-a) or mixtures thereof, and, if present, the one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof, in the polyester is at least 50 wt.-%, preferably is at least 60 wt.-% and more preferably is at least 70 wt.-%, in each case based on the total weight of the polyester.

[0219] 15. The use according to one or more of paragraphs 1 to 14, characterized in that the weight average molecular weight (Mw) of the soil release polymer or of the polyester is from 1500 to 20000 g / mol and preferably from 5000 to 20000 g / mol.

[0220] 16. The use according to one or more of paragraphs 6 to 15, characterized in that the polyester either a) consists of a1) one or more structural units of the formula (I) and a2) one or more structural units of the formula (II) or mixtures thereof and a3) one or more terminal groups of the formula (lll-a) or mixtures thereof, or b) consists of b1) one or more structural units of the formula (I) and b2) one or more structural units of the formula (II) or mixtures thereof and b3) one or more terminal groups of the formula (lll-a) or mixtures thereof, and b4) one or more polyalkyleneglycol-derived structural units of the formula (IV-a) or mixtures thereof.

[0221] 17. The use according to one or more of paragraphs 1 to 16, characterized in that the soil release polymer or the polyester is anionic or nonionic and preferably is nonionic.

[0222] 18. The use according to one or more of paragraphs 1 to 17, characterized in that the textile comprises or consists essentially of polyester or another synthetic material and preferably comprises or consists essentially of polyester.

[0223] 19. The use according to one or more of paragraphs 1 to 18, characterized in that the polypeptide having DNase activity and the soil release polymer or the polyester are present in a laundry detergent composition.

[0224] 20. A laundry detergent composition comprising

[0225] Z1) one or more polypeptides having DNase activity and having at least 80% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, and

[0226] Z2) one or more terephthalic acid-based soil release polymers.

[0227] 21. The laundry detergent composition according to paragraph 20, characterized in that the one or more polypeptides having DNase activity and having at least 80% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3 of component Z1) are polypeptides as described in paragraph 2 or 3.

[0228] 22. The laundry detergent composition according to paragraph 20, characterized in that the one or more polypeptides having DNase activity and having at least 80% sequence identity to SEQ ID NO: 4 or 5 of component Z1) are polypeptides as described in paragraph 4 or 5.

[0229] 23. The laundry detergent composition according to one or more of paragraphs 20 to 22, characterized in that it comprises the one or more polypeptides having DNase activity and having at least 80% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, of component Z1) in an amount of up to about 10,000 ppm, for example from 1 ppm to 10,000 ppm, such as from 10 ppm to 5000 ppm, from 20 ppm to 2000 ppm, from 50 ppm to 1000 ppm, from 80 ppm to 600 ppm or from 100 ppm to 500 ppm, in each case based on the total weight of the laundry detergent composition.

[0230] 24. The laundry detergent composition according to one or more of clams 20 to 23, characterized in that the one or more terephthalic acid-based soil release polymers of component Z2) are polyesters as described in one or more of paragraphs 6 to 17.

[0231] 25. The laundry detergent composition according to one or more of paragraphs 20 to 24, characterized in that it comprises the one or more terephthalic acid-based soil release polymers of component Z2) in an amount of at least 0.1 wt.-%, preferably in an amount from 0.1 to 10 wt.- %, more preferably in an amount from 0.2 to 5 wt.-% and even more preferably in an amount from 0.2 to 3 wt.-%, in each case based on the total weight of the laundry detergent composition.

[0232] 26. The laundry detergent composition according to one or more of paragraphs 20 to 25, characterized in that it comprises

[0233] Z3) one or more surfactants.

[0234] 27. The laundry detergent composition according to paragraph 26, characterized in that it comprises the one or more surfactants of component Z3) in an amount of at least 3 wt.-%, preferably in an amount from 3 to 65 wt.-%, more preferably in an amount from 4 to 60 wt.-% and even more preferably in an amount from 5 to 55 wt.-%, in each case based on the total weight of the laundry detergent composition.

[0235] 28. A method for laundering a textile, comprising exposing the textile to a wash liquor comprising a polypeptide having DNase activity and a soil release polymer, completing at least one wash cycle, and optionally rinsing the textile.

[0236] 29. A method for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintain ing or improving whiteness of a textile, comprising exposing the textile to a wash liquor comprising a polypeptide having DNase activity and a soil release polymer, completing at least one wash cycle, and optionally rinsing the textile. 30. The method according to paragraph 28 or 29, wherein the polypeptide having DNase activity is as defined in any of paragraphs 2-5, and / or wherein the soil release polymer is as defined in any of paragraphs 6-17, and / or wherein the textile is as defined in paragraph 18.

[0237] 31 . The method according to any of paragraphs 28-30, wherein the polypeptide having DNase activity and the soil release polymer are present in a laundry detergent composition as defined in any of paragraphs 20-27.

[0238] Further preferred embodiments of the invention may arise from the combination of abovedescribed preferred embodiments.

[0239] EXAMPLES

[0240] The examples below are intended to illustrate the invention in detail without, however, limiting it thereto. Unless explicitly stated otherwise, all percentages given are percentages by weight (weight%, % by wt. or wt.-%).

[0241] Assay I: Testing of DNase activity

[0242] DNase activity may be determined on DNase Test Agar with Methyl Green (BD, Franklin Lakes, NJ, USA), which is prepared according to the supplier’s manual. Briefly, 21 g of agar is dissolved in 500 ml water and then autoclaved for 15 min at 121 °C. Autoclaved agar is adjusted to 48°C in a water bath, and 20 ml of agar is poured into petri dishes and allowed to solidify by incubation overnight at room temperature. On solidified agar plates, 5 pl of enzyme solutions are added and DNase activity is observed as colorless zones around the spotted enzyme solutions.

[0243] Example 1 - Preparation of liquid laundry detergent compositions

[0244] Liquid detergent compositions according to Table 1 were prepared, where amounts of the components are given in weight% by total weight of the composition, except for the DNase, where the amount is given in ppm active enzyme product based on the total weight of the composition.

[0245] Table 1 . Liquid laundry detergent compositions A, B, C and D

[0246] 1A commercially available nonionic soil release polymer

[0247] Example 2 - Preparation of sebum / DNA mixture

[0248] 1. Preparation of DNA solution:

[0249] Salmon DNA (1 g, CAS no. 438545-06-3, purchased from Sigma) was weighted in a beaker. Demineralized water (99 g) was added to prepare a final solution, which contains 1 weight% of DNA. The mixture was stirred on a magnet stirrer for 1 hour at 400 rpm (rpm: resolution per minute), at 70°C, until completely dissolved. The beaker was covered closely with aluminum foil to avoid evaporation while stirring.

[0250] 2. Preparation of sebum / DNA mixture:

[0251] Tween 80 (Polysorbate 80, 200 pL) was added to the DNA solution (100 g) just before adding 0.3 g Sebum Bey (purchased from CFT), so that the final DNA solution contains 0.2 weight%

[0252] Tween 80. Sebum Bey (0.3 g) was then added to the DNA solution. The mixture was stirred at 400 rpm at 70°C for 5-10 minutes and then ULTRA-TURRAX at a high mix speed of 16000 rpm was applied to the mixture for 1 minute. The mixture was afterwards cooled to room temperature and was used within 3 days.

[0253] Example 3 - Preparation of DNA / sebum soiled swatches for malodor removal from primary wash and reducing redeposition of malodor on a textile

[0254] DNA / sebum mixture (200 pL) from Example 2 was added on each swatch (knitted polyester, WFK 33A, 6 cm x 6 cm). The strains were left to dry overnight in the dark at room temperature before being used in the following experiments.

[0255] Example 4 - Preparation of DNA / sebum soiled swatches for malodor repellent

[0256] Swatches (knitted polyester, WFK 33A, 6 cm x 6 cm) were pre-washed 3 times with liquid laundry detergent compositions A, B, C and D under the conditions listed in Table 2.

[0257] Table 2. Pre-wash conditions of swatches

[0258] Pre-washed swatches were then rinsed under demineralized water. Excess of water was then wringed out. The swatches were then left to dry overnight. DNA / sebum mixture (200 pL) from Example 2 was then added on each dried pre-washed swatch. The stains were left to dry overnight in the dark at room temperature before being used in the following experiments.

[0259] Example 5 - Preparation of malodor soiled test swatches malodor removal from primary wash and reducing redeposition of malodor on a textile

[0260] Artificial malodor composition (50 pL) was dosed on top of the sebum / DNA stain on each dried sebum / DNA soiled swatch from Example 3 (prepared without pre-wash) and Example 4 (prepared with pre-wash). The swatches were then left to dry for 30 minutes in a fume hood at room temperature.

[0261] Artificial malodor composition: The artificial malodor composition was prepared by mixing 50 mg of 2,3-butanedione, 25 mg of octanal, 15 mg of isovaleraldehyde, 1500 mg of isovaleric acid (IVA) and 250 mg of hexanal with 500 g of water and 500 g of isopropanol. The artificial malodor composition was prepared and used on the same day.

[0262] Example 6 - Fabric washing for malodor removal from primary wash

[0263] Washing of fabrics:

[0264] Malodor soiled swatches from Example 5 (prepared without pre-wash) were then washed with liquid laundry detergent compositions A, B, C and D under the conditions shown in Table 3.

[0265] Table 3. Washing conditions for malodor removal from primary wash

[0266] Test swatches were rinsed under tap water 3 times and then under demineralized water 3 times after wash. Excess water was then wringed out.

[0267] Analytical procedure:

[0268] The washed swatches were then packed into Headspace GC vials without further drying steps and the vials were then closed tightly and analyzed.

[0269] Analytical evaluation of malodor in the vials was conducted through a headspace measurement. A Gas Chromatograph Agilent 9000 Intuvo equipped with an autosampler Agilent 7697A equipped with a headspace extracting syringe, a Mass Detector Agilent MSD 5977B was used. The headspace vials were equilibrated at 60°C for 30 minutes at the autosampler before the measurement. A gas phase sample of 1000 pL was extracted from the vial and injected into the GC column.

[0270] Carrier gas: Helium 22.7 psi, split 5:1

[0271] Column: DB-624 III (length 60 m, diameter 250 pm, size of film 1.4 pm)

[0272] Oven: Initial 40°C - Hold time 2 minutes - Rate 20 °C / minute - Temperature 260°C - Hold time 5 minutes Mass detector parameter: run in select ion mode. The peak area of an odorant, Hexanal as marker, was then integrated and recorded.

[0273] Results are shown in Table 4.

[0274] Table 4. Relative peak areas* for the marker odorant (Hexanal) measured in sample headspace from fabric washed for malodor removal from primary wash

[0275] * The peak areas of the individual odorants in the example containing fabrics washed with liquid laundry detergent composition A were normalized as 100.

[0276] From Table 4, it can be clearly seen that the malodor intensity from the fabrics washed with the liquid laundry detergent composition D (inventive) was significantly reduced.

[0277] Example 7 - Fabric washing for reducing redeposition of malodor on a textile

[0278] Washing of fabrics:

[0279] Malodor soiled swatches from Example 5 (prepared without pre-wash) and fresh unsoiled swatches were then washed with liquid laundry detergent compositions A, B, C and D under the conditions shown in Table 5.

[0280] Table 5. Washing conditions for reducing redeposition of malodor on a textile Washed fresh unsoiled swatches were rinsed under tap water 3 times and then under demineralized water 3 times after wash. Excess water was then wringed out.

[0281] Analytical procedure:

[0282] Same as described in Example 6.

[0283] Results are shown in Table 6.

[0284] Table 6. Relative peak areas* for the marker odorant (Hexanal) measured in sample headspace from fabric washed for reducing redeposition of malodor on a textile

[0285] * The peak areas of the individual odorants in the example containing fabrics washed with liquid laundry detergent composition A were normalized as 100.

[0286] From Table 6, it can be clearly seen that the malodor intensity from the fabrics washed with the liquid laundry detergent composition D (inventive) is not detectable anymore, which means no malodor was redeposited onto the fresh unsoiled swatches anymore.

[0287] Example 8 - Fabric washing for malodor repellent

[0288] Washing of fabrics:

[0289] Malodor soiled swatches from Example 5 (prepared with pre-wash) were then washed with liquid laundry detergent compositions A, B, C and D under the conditions shown in Table 7.

[0290] Table 7. Washing conditions for malodor repellent Washed swatches were rinsed under tap water 3 times and then under demineralized water 3 times after wash. Excess water was then wringed out.

[0291] Analytical procedure:

[0292] Same as described in Example 6.

[0293] Results are shown in Table 8.

[0294] Table 8. Relative peak areas* for marker odorant (Hexanal) measured in sample headspace from fabric washed for malodor repellent

[0295] * The peak areas of the individual odorants in the example containing fabrics washed with liquid laundry detergent composition A were normalized as 100.

[0296] From Table 8, it can be clearly seen that the malodor intensity from the fabrics washed with the liquid laundry detergent composition D (inventive) is not detectable anymore, which means all malodor was removed by washing and the swatches pre-washed with liquid laundry detergent D had good malodor repellent effect.

[0297] Example 9: Whiteness benefit of soil release polymer (SRP) and DNase

[0298] Fabric

[0299] Woven polyester / cotton (65% polyester 135% cotton), WFK 20A (Center For Testmaterials BV, the Netherlands), was prewashed as described below and cut into small round swatches with a diameter of 2 cm.

[0300] Prewash without SRP

[0301] Prewash of textiles was done primarily to remove starch, carboxymethyl cellulose (CMC) and other additives from the textile. Amylase (Stainzyme® 12L, Novozymes A / S, Denmark), cellulase (Celluclean® ST, Novozymes) and protease (Savinase® 16L, Novozymes) were added to the prewashes to remove these additives. The textiles were washed three times in 86.1 g / wash detergent W-ECE-2 (wfk Testgewebe GmbH, Germany) using water with 15°dH water hardness (3.00 mL of 0.713 mol / L CaCh, 1.50 mL of 0.357 mol / L MgCh and 0.3371 g of NaHCOs in 1 L deionized water) and containing the following enzymes:

[0302] Prewash with SRP

[0303] The above pre-washed textile was washed three times in 5 g / L of liquid detergent (composition A in Table 1 , Example 1) containing 2% of a soil release polymer, TexCare® SRN 260 (Clariant, Germany), using water with 15°dH water hardness as described above, at 40°C. After wash, the textile was line-dried and cut into small round swatches with diameter of 2 cm.

[0304] Preparation of microbial extracellular DNA (eDNA) stain

[0305] Pseudomonas fluorescens was pre-grown on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131 , Oxoid Lid. Basurgstukc, UK) for 3 days at 30°C. From a single colony, a loop-full was transferred to each of the 3 tubes containing 10 mL of Tryptone Soya Broth (TSB) and incubated for 1 day at 30°C with shaking (200 rpm). The optical density (OD) at 600 nm (ODeoo) was measured using a Spectrophotometer (Clariostar, BMG Labtech, Germany). The bacteria culture was diluted to an ODeoo of 0.03 using half-strength TSB, and 200 ml of the diluted culture was transferred into one 500 mL sterile flask (4 flasks in total). The flasks with Pseudomonas fluorescens were incubated at 30°C for 3 days with shaking at 120 rpm on a sharker (MaxQ™ 4000).

[0306] After propagation, Pseudomonas fluorescens was pelleted by centrifugation (Sorvall LYNX 6000) (10,000 rpm for 10 min) and resuspended in 2 mL of phosphate buffered saline (PBS) (pH 6.9). All the resuspended pellets were transferred into one tube. This tube with resuspended bacteria culture was stored in a cold room and used for all four washes in the multi-cycle wash described below. Before each wash, the ODeoo value of the culture was measured on the spectrophotometer (Clariostar), and was diluted to reach ODeoo = 2 using PBS. For each wash, every swatch was spotted with 50 pl bacteria culture (ODeoo = 2) and was then dried in a clean bench for one hour.

[0307] Detergent

[0308] The detergent was the liquid detergent of composition A described above in Table 1 of Example 1.

[0309] Wash assay - Multi-cycle Mini Launder-O-Meter (miniLOM)

[0310] The miniLOM assay is a small-scale version of the Launder-O-Meter (LOM). A MiniLOM basically consists of closed test tubes being rotated in a heating cabinet for a given time and temperature. Each test tube represents one small washing machine. It is mainly used in testing of detergents and enzymes at European wash conditions.

[0311] In this experiment, three microbial eDNA stains prewashed without SRP were placed in a 50-ml plastic tube and 10 ml of wash solution (15° dH water with 4.3 g / L liquid detergent and 0.7 g / L pigment soil (I LG 09V (wfk Tesgewebe GMB, Germany)) was added to each tube. The following four wash conditions were tested: 1) detergent only (no SRP, no DNase); 2) only SRP, no DNase; 3) no SRP, only DNase; and 4) both SRP and DNase. The SRP concentration was 1% by weight of the detergent, and the DNase concentration in the wash solution was 0.5 ppm.

[0312] The DNase used in this example is a variant of SEQ ID NO: 1 with the substitutions D32E, V35I, K65E, K67A, K105N, A111 P, G181 N and S182Y, where position numbers are based on SEQ ID NO: 1 . The SRP was TexCare® SRN 260.

[0313] The tubes were then mounted in a Mini-Laundr-O-Meter (a Stuart Tube Rotator SB3) and washed at 30°C for 60 minutes at 20 rpm. After wash, the rotator was placed at room temperature while swatches from one tube at a time were rinsed with 15°dH water and placed back into the rotator. Each plastic tube was rinsed two times in 20 mL 15°dH water. After wash, the swatches were dried and evaluated as described below. The whole process (adding culture to swatches, washing, evaluation) was repeated four times in total.

[0314] In parallel, the multi-cycle MiniLOM washes were repeated with the microbial eDNA stains prewashed with SRP under the same wash conditions and wash cycles as described above.

[0315] Evaluation of the whiteness benefits

[0316] After drying, all swatches were measured on a Datacolor 800V spectrophotometer (Datacolor, Lawrenceville, NJ, USA) using the CIE Standard llluminant D65 and the CIE 1964 10° Standard Observer. The measurements were made without UV in the incident light, and remission values at 460 nm were recorded for the stains. The whiteness benefits were visually evident when there was a remission difference between stains of two units or more. Results

[0317] The results are shown in Tables 9 and 10 below, where Table 9 provides the results for swatches that were pre-washed without SRP, and Table 10 provides the results for swatches that were pre-washed with SRP. The “SRP benefit”, “DNase benefit” and “SRP+DNase benefit” were calculated by subtracting the remission value of condition 1 (detergent only) from the remission values of condition 2, 3 and 4, respectively. The “Presumed additive benefit of SRP+DNase” was calculated by adding the values of “SRP benefit” and “DNase benefit”. The “Difference between experimental and presumed additive benefit of SRP+DNase” was calculated by subtracting the value of “Presumed additive benefit of SRP+DNase” from the value of “SRP+DNase benefit”, and represents the synergistic benefit of the combination of the soil release polymer and the DNase.

[0318] Table 9: Remission values (460 nm) measured for swatches pre-washed without SRP after 1, 2,

[0319] 3 or 4 wash cycles with SRP and / or DNase Table 10: Remission values (460 nm) measured for swatches pre-washed with SRP after 1 , 2, 3 or 4 wash cycles with SRP and / or DNase

[0320] The data in Tables 9 and 10 show that the combination of SRP and DNase provided a synergistic effect on whiteness in the multi-cycle wash for both types of stains, i.e. prewashed with or without SRP, starting from the 2ndwash cycle. It is also seen that the synergistic effect of the combination increases with each additional wash using SRP and DNase from the 2ndto the 4thwash cycle. This synergistic effect starting from the 2ndwash cycle was observed with both swatches pre-washed without SRP and those pre-washed with SRP, although the benefit was greater for swatches pre-washed with SRP.

Claims

Patent Claims:

1. Use of a polypeptide having DNase activity and a soil release polymer in a laundry process for reducing redeposition of malodor on a textile, for removing malodor from a textile and / or for maintaining or improving whiteness of a textile.

2. The use according to claim 1, characterized in that the polypeptide having DNase activity has at least 80% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16 or SEQ ID NO: 17, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, 14, 15, 16 or 17.

3. The use according to claim 2, characterized in that the polypeptide having DNase activity comprises the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or is a variant of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, wherein the variant has at least 80% but less than 100% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% or at least 95% sequence identity to SEQ ID NO: 1, 2 or 3; and preferably wherein said variant comprises one or more alterations selected from the group consisting of: a) one or more substitutions selected from the group consisting of N4E, L17E, T19A, T19G, T19I, K36P, Q38P, S39V, S39R, A40P, A40H, L41T, L41 H, V45H, L51G, K53T, K53P, G54P, A55P, N57H, E64A, E64Q, E64R, E64T, E64I, E64S, T66H, K67A, K67T, N68V, N68P, N68I, N68H, S69A, S69D, S69E, S69K, S69L, S69W, S69Y, S69Q, N70T, N70H, N70G, R71T, D72E, S74H, S74G, G75I, N77T, K82P, K82I, D83T, D83P, D83I, D83H, D83G, P84H, Q85T, Q85P, Q85H, K86T, K86P, K86H, G88P, G88H, A91P, W99T, A101W, K105E, K105N, K105T, K105D, S106T, S115T, L116I, Q135L, G136L, V138I, V138L, V138P, V138Q, L139A, N140R, N140L, N140A, G141L, F151 R, D152Y, D152L, D152I, D152A, P153E, S154R, T162R, W163E, F164R, I166Y, I166R, K168N, F169R, F169E, A173I, A173R, A173T, S182R, N183E, D184I, K185Y, S186I, D189G, D189H, K212G, K212P and K215I; b) two or more alterations selected from the group consisting of S69V, Q102E, S26*, D32Q, K36C,H, G37R, F43W, D46G, A55I, N68D, A76I, K82S,T, P84D,T, K86G,L,N,Q,T,V,Y, A91R, L92E, K95I, P97E,N, A101 E, K105N,G,Q,T,D, F112Y.W,L129K, N133Q, V138C, N140H, G141Q.R, S144E, N146A, K147N.E, V148I, A149D,E,F, Q150D, P153D.V, S154E, K155E,F,L,S,T, Q157D.E, Q158D, T159Q, K160D, T170Q, A172D,E,H,R, K185*, V187N.Y, N191*, K192A, D197K.S, G199Q, Q208V, E211Y,T,P, N213S, N214D, N217A and Y218D.E; and c) at least two substitutions, such as at least three substitutions, selected from the group consisting of A111 P, D32E, V35I, S69V, Q102E, K105N and G181 N; and d) the substitution A111 P and two or more substitutions selected from the group consisting of S26H, D32E, V35I, K65E, K67A, S69E, S69V, S98R, Q102E, K105N, S115T, Q150E, Q157E, T159Q, G161R, A172E, G181 N, S182Y, S182V, K185E, V187N, K192I, A206G, Q208V and K212E, wherein position numbers are based on the numbering of SEQ ID NO: 1.

4. The use according to claim 2, characterized in that the polypeptide having DNase activity has at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4.

5. The use according to claim 2, characterized in that the polypeptide having DNase activity is a variant of SEQ ID NO: 4, wherein the variant has at least 80% sequence identity to SEQ ID NO: 5, for example at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 5; and preferably wherein said variant comprises one or more substitutions selected from the group consisting of: a) one or more substitutions selected from the group consisting of T11, T1V, T1Y, T1M, T1 E, G4N, T5F, T5C, P6V, P6G, S7D, S7T, K8V, S9K, S9Q, S9V, S9L, S9F, S9P, S9R, A10D, A10M, A10I, A10Q, A10V, A10L, A10K, Q12S, Q12V, Q12E, S13D, S13Y, S13Q, S13F, S13R, S13V, S13N, S13H, S13M, S13W, S13K, S13L, S13E, Q14M, Q14R, N16S, A17C, A17V, A17E, A17T, T19K, T19L, T19S, T19I, T19V, K21 E, K21M, T22P, T22A, T22V, T22D, T22R, T22K, T22M, T22E, T22H, T22L, T22W, T22F, T22C, T22I, G24Y, S25P, S27N, S27I, S27M, S27D, S27V, S27F, S27A, S27C, S27L, S27E, G28L, Y29W, S30K, S30D, S30H, S30T, D32Q, I38V, I38M, S39A, S39P, S39Y, S39H, S39E, S39N, S39M, S39D,Q40V, S42C, S42L, S42M, S42F, S42W, V49R, L511, K52I, K52H, A55S, D56I, D56L, D56T, S57W, S57F, S57H, S57C, S57P, S57V, S57R, S57T, Y58A, Y58T, S59C, S59T, S59L, S59Q, S59V, S59K, S59R, S59M, S59I, S59H, N61 D, P63A, T65L, T65I, T65V, T65R, T65K, S68V, S68I, S68W, S68Y, S68H, S68C, S68T, S68L, V76G, V76L, V76C, V76K, V76H, V76E, V76A, V76Y, V76N, V76M, V76R, V76F, T77N,T77Y, T77W, T77R, F78I, F78H, F78Y, F78C, T79G, T79R, N80K, S82L, S82E, S82K, S82R, S82H, D83C, D83F, D83L, L92T, A93G, E94N, G99S, S101D, S101A, S102M, S102L, S102V, S102A, S102K, S102T, S102R, T104P, T104A, T105V, T105I, K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, K107D, Q109R, Q109S, A112S, S116D, S116R, S116Q, S116H, S116V, S116A, S116E, S116K, A125K, S126I, S126E, S126A, S126C, T127C, T127V, S130E, G132R, D135R, T138Q, W139R, R143E, R143K, S144Q, S144H, S144L, S144P, S144E, S144K, G145V, G145E, G145D, G145A, A147Q, A147W, A147S, G149S, K152H, K152R, S156C, S156G, S156K, S156R, S156T, S156A, T157S, Y159F, K160V, W161L, W161Y, G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, G162H, S164R, S164T, Q166D, S167M, S167L, S167F, S167W, S167E, S167A, S167Y, S167H, S167C, S167I, S167Q, S167V, S167T, S168V, S168E, S168D, S168L, K170S, K170L, K170F, K170R, T171 D, T171 E, T171A, T171C, A172G, A172S, L173T, L173A, L173V, Q174L, G175D, G175E, G175N, G175R, M176H, L177I, N178D, N178E, N178T, N178S, N178A, S179E, S181R, S181E, S181D, S181 F, S181H, S181W, S181 L, S181M, S181Y, S181Q, S181G, S181A, Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y182I, Y182E, Y182T and Y182W; b) two or more substitutions selected from the group consisting of T1 I, T1L, T1V, S13Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T127I, T127L, S144P, A147H, S167L, S167I, S167V, G175D and G175E; and c) two or more substitutions selected from the group consisting of N61 D, T65I, T65V, S82R, K107Q, T127S, T127V, G149N and S164D, and preferably at least one substitution selected from the group consisting of Q14R, Q14W, K21 L, L33K, Q48D, D56I, D56L, S66Y, S68L, S102Y, S106A, Q109T, S116W, T171W, S181T and S181W; wherein position numbers are based on the numbering of SEQ ID NO: 4.

6. The use according to one or more of claims 1 to 5, characterized in that the soil release polymer is a polyester comprising one or more structural units of the formula (I)and one or more structural units of the formula (II) or mixtures thereof-O(CnH2n)O- (II) wherein (CnH2n) is a linear or branched alkylene group with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (C He), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (C He) and mixtures thereof, and particularly preferably is (CaHe), and one or more terminal groups of the formula (lll-a) or mixtures thereof(lll-a)whereinR1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 30 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 30 carbon atoms or mixtures thereof, preferably a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 carbon atoms or a linear or branched, preferably a linear, alkenyl group comprising one or more double bonds and from 2 to 20 carbon atoms or mixtures thereof, a is, based on a molar average, a number from 1 to 200, preferably from 10 to 200, more preferably from 10 to 150, andR2is a linear or branched alkylene group (CmH2m) with m being a number from 2 to 10 or mixtures thereof, preferably with m being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CaHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (C He) and mixtures thereof, and particularly preferably is (C2H4).

7. The use according to claim 6, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 2 to 60, preferably from 2 to 40, more preferably from 2 to 30, even more preferably from 2 to 20 and particularly preferably from 5 to 20.

8. The use according to claim 6 or 7, characterized in that the number of structural units of the formula (I) in the polyester is, based on a molar average, from 2 to 15, R1is a linear or branched, preferably a linear, alkyl group comprising from 1 to 20 and preferably from 1 to 18carbon atoms, or mixtures thereof, and a is, based on a molar average, a number from 10 to 150, preferably from 15 to 140.

9. The use according to one or more of claims 6 to 8, characterized in that the polyester comprises one or more polyalkyleneglycol-derived structural units of the formula (IV-a)or mixtures thereof, wherein f is, based on a molar average, a number from 2 to 200, preferably from 10 to 200, more preferably from 20 to 150 and even more preferably from 20 to 120, andR3is a linear or branched alkylene group (CnH2n), with n being a number from 2 to 10 or mixtures thereof, preferably with n being a number from 2 to 6 or mixtures thereof, more preferably is selected from the group consisting of (C2H4), (CsHe), (C4H8) and mixtures thereof, even more preferably is selected from the group consisting of (C2H4), (CsHe) and mixtures thereof, and particularly preferably is (C2H4).

10. The use according to one or more of claims 1 to 9, characterized in that the textile comprises or consists essentially of polyester or another synthetic material and preferably comprises or consists essentially of polyester.

11. A laundry detergent composition comprisingZ1) one or more polypeptides having DNase activity with at least 80% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3, or having at least 80% sequence identity to SEQ ID NO: 4 or 5, andZ2) one or more terephthalic acid-based soil release polymers.

12. The laundry detergent composition according to claim 11 , characterized in that the one or more polypeptides having DNase activity with at least 80% sequence identity to SEQ ID NO:1 , SEQ ID NO: 2 or SEQ ID NO: 3 of component Z1) are a) polypeptides as described in claim 2 or 3, or b) polypeptides as described in claim 4 or 5.

13. The laundry detergent composition according to claim 11 or 12, characterized in that it comprises the one or more polypeptides having DNase activity with at least 80% sequence identity to SEQ ID NO: 1 , SEQ ID NO: 2 or SEQ ID NO: 3, or having at least 80% sequenceidentity to SEQ ID NO: 4 or 5, of component Z1) in an amount of up to about 10,000 ppm, for example from 1 ppm to 10,000 ppm, such as from 10 ppm to 5000 ppm, from 20 ppm to 2000 ppm, from 50 ppm to 1000 ppm, from 80 ppm to 600 ppm or from 100 ppm to 500 ppm, in each case based on the total weight of the laundry detergent composition.

14. The laundry detergent composition according to one or more of clams 11 to 13, characterized in that the one or more terephthalic acid-based soil release polymers of component Z2) are polyesters as described in one or more of claims 6 to 9.

15. The laundry detergent composition according to one or more of claims 11 to 14, characterized in that it comprises the one or more terephthalic acid-based soil release polymers of component Z2) in an amount of at least 0.1 wt.-%, preferably in an amount from 0.1 to 10 wt.- %, more preferably in an amount from 0.2 to 5 wt.-% and even more preferably in an amount from 0.2 to 3 wt.-%, in each case based on the total weight of the laundry detergent composition.

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