Composition for controlling banana diseases, comprising organic iodine and sulfur as active ingredients

Abstract

The present invention relates to a composition for controlling banana diseases, comprising organic iodine (I)-sulfur (S) as an active ingredient, wherein the organic iodine (I)-sulfur (S) is formed by the organic chelation bonding of an organic substrate to the iodine (I) and sulfur (S). Therefore, fungal diseases including Panama disease occurring in bananas, bacterial diseases caused by gram-negative bacteria, and viral diseases can be effectively controlled.

Description

Composition for controlling banana disease containing organic iodine and sulfur as active ingredients

[0001] The present invention relates to a composition for controlling banana diseases, and more particularly, to a composition for controlling banana diseases caused by fungi, bacteria, or viruses.

[0002] The banana industry has recently been struggling with various disease-causing fungi, bacteria, and pests. The most problematic of these is Panama disease, also known as banana blight, caused by the fungus Fusarium oxysporum. Prior to the 1960s, the banana industry relied on the large, sugar-rich "Gros Michel" variety, but this fungal disease largely drove it out of the market. Since then, the Cavendish variety has replaced it, accounting for approximately 80% of bananas exported.

[0003] Fusarium oxysporum usually lives in the soil, but it penetrates through the roots and spreads throughout the plant through the water vessels to reproduce. During this process, the fungal hyphae block the vascular system of the banana plant, cutting off the water supply, causing the banana leaves to turn yellow and wither and die. Fusarium oxysporum was first discovered in 1903, and the strain causing the problem was 'Race One' (R1). It quickly spread to neighboring countries and eventually caused the disappearance of the Gros Michel variety in 1960. The fungus that causes Panama disease in bananas is 'Tropical Racepo' (TR4). 'Tropical Racepo' (TR4) is also causing the disease in Cavendish bananas, which are known to be resistant to Panama disease, so a solution is needed.

[0004] In addition, fungal diseases such as black Sigatoka disease caused by Mycosphaerella fijiensis and yellow Sigatoka disease caused by Mycosphaerella musicola, bacterial diseases such as fire blight and soft rot caused by Erwinia sp. and yellow spot disease caused by Pseudomonas sp., and viral diseases such as Banana bunchy top virus (BBTV) and Banana bract mosaic virus (BBMV) are causing difficulties in banana cultivation.

[0005] Accordingly, there is a need to develop a banana disease control agent that can effectively control banana diseases caused by fungi, bacteria, and viruses.

[0006] The purpose of the present invention is to provide a composition for controlling banana diseases, which contains organically passivated iodine (I)-sulfur (S) as an effective ingredient, which can effectively control fungal banana diseases, including Panama disease, and banana diseases caused by bacteria or viruses caused by Gram-negative bacteria.

[0007] According to one aspect of the present invention,

[0008] A composition for controlling banana disease is provided, which comprises organically passivated iodine (I)-sulfur (S) as an active ingredient, and wherein the organically passivated iodine (I)-sulfur (S) is characterized in that an organic substrate is organically passivated by chelating with the iodine (I) and sulfur (S).

[0009] In the above organic iodine(I)-sulfur(S), the weight ratio of iodine(I) and sulfur(S) can be 10:0.5 to 10:2.

[0010] The organic substrate chelated to the above iodine (I) and sulfur (S) may be any one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and methyl tolysulfon.

[0011] The above composition for controlling banana disease further comprises organically passivated iodine (I), and the organically passivated iodine (I) may be organically passivated by chelating an organic substrate to the iodine (I).

[0012] The organic substrate chelated to the organic iodine (I) may be any one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and propynyl butyl carbamate.

[0013] The above organically passivated iodine (I)-sulfur (S) may be prepared into a water-soluble formulation by mixing with any one solvent selected from butyldiol and polyethylene glycol.

[0014] The above composition for controlling banana disease may additionally contain glycomacropeptide (GMP).

[0015]

[0016] *The above banana disease is a fungal disease, and the fungus causing the fungal disease may be any one selected from among Fusarium oxysporum, Mycosphaerella fijiensis, Mycosphaerella musae, Mycosphaerella musicola, Cordana musae, Guignardia musae, Colletotrichum musae, Caldosporium musae, Verticillium sp., and Chloridium spp.

[0017] The above banana disease is a bacterial disease, and the bacteria causing the bacterial disease may be any one selected from Erwinia sp. and Pseudomonas sp.

[0018] The above banana disease is a viral disease, and the virus causing the viral disease may be any one selected from among BBTV (Banana bunchy top virus), BBMV (Banana bract mosaic virus), CMV (Cucumber mosaic virus), and BSV (Banana streak virus).

[0019] The above composition for controlling banana disease may additionally include a coating inhibitor.

[0020] According to another aspect of the present invention,

[0021] A method for controlling banana disease using the above composition for controlling banana disease is provided.

[0022] The composition for controlling banana diseases comprising organic passivating iodine and sulfur of the present invention as effective ingredients can effectively control banana diseases caused by fungi, including Panama disease, and banana diseases caused by bacteria or viruses caused by Gram-negative bacteria.

[0023] Figure 1 is a photograph of bacteria and fungi that cause banana disease at each harvest site.

[0024] Figure 2 shows the results of pure isolation of white-type Erwinia sp. (a) and yellow-type Pseudomonas sp. (b) bacteria, respectively.

[0025] Figure 3 is a photograph of the mixed strain treated at different concentrations according to Experimental Example 1.

[0026] Figure 4 is a photograph showing the results of treatment with different concentrations of mineral preparations for the mixed strain of Experimental Example 1.

[0027] Figure 5 is a photograph showing the results of treatment with different concentrations of mineral preparations for the Erwinia genus (Erwinia. sp.) strain of Experimental Example 1.

[0028] Figure 6 is a photograph showing the results of treatment with different concentrations of mineral preparations for the Pseudomonas sp. strain of Experimental Example 1.

[0029] Figure 7 is a photograph showing the results of an analysis of the instantaneous contact control efficacy of a mineral preparation against the fungus Fusarium oxysporum in Experimental Example 1.

[0030] Figure 8 shows the final results of evaluating bacterial resistance and the inhibitory ability of mineral preparations against the fungus Fusarium oxysporum according to Experimental Example 2.

[0031] Figure 9 shows the daily trend of the evaluation of bacterial resistance and the inhibitory ability of mineral preparations against the fungus Fusarium oxysporum according to Experimental Example 2.

[0032] Figure 10 shows the results of analysis of the Erwinia amylovora growth inhibition ability of the mineral preparation of Example 1 according to Experimental Example 3.

[0033] Figure 11 shows the results of analysis of the Erwinia amylovora growth inhibition ability of the mineral preparation of Example 2 according to Experimental Example 3.

[0034] Figure 12 shows the results of analysis of the control ability against Erwinia amylovora according to the concentration and contact time of the mineral preparation of Example 1 according to Experimental Example 3.

[0035] Figure 13 is a photo of a banana infected with BBTV (Banana bunchy top virus).

[0036] Figure 14 is a photograph taken 4 weeks after bananas were transplanted to the infected field in Experimental Example 5.

[0037] Figure 15 is a photograph taken 10 days after the first treatment with the composite formulation of Example 4 in Experimental Example 5.

[0038] Figure 16 is a photograph taken 10 days after the second treatment with the compound formulation of Example 4 in Experimental Example 5.

[0039] Figure 17 is a photograph taken 25 days after the second treatment with the compound formulation of Example 4 in Experimental Example 5.

[0040] Figure 18 is a photograph taken 45 days after the second treatment with the compound formulation of Example 4 in Experimental Example 5.

[0041] Figure 19 is a photograph comparing a banana harvesting area in Experimental Example 5 with a control farm that was not treated with the composite formulation of Example 4.

[0042] Figure 20 is a photograph taken during banana harvesting in Experimental Example 5.

[0043] Figure 21 is a photograph of a banana seedling that has gone through the seedling stage of Experimental Example 6.

[0044] Figure 22 is a comparative photograph of a conventional cultivation plot that was not treated with the compound formulation of Example 4 and a photograph taken 18 days after the formal treatment of Experimental Example 6.

[0045] Figure 23 is a photograph comparing the state of bananas approximately two months after the formal application of Experimental Example 6 with the control group.

[0046] Hereinafter, the composition for controlling banana disease of the present invention will be described.

[0047] The composition for controlling banana disease of the present invention comprises organically passivated iodine (I)-sulfur (S) as an effective ingredient, and the organically passivated iodine (I)-sulfur (S) is characterized in that an organic substrate is organically passivated by chelating with the iodine (I) and sulfur (S).

[0048] The above organic iodine (I)-sulfur (S) preferably has a weight ratio of iodine (I) to sulfur (S) of 10:0.5 to 10:2, and more preferably 10:1 to 10:1.5. The banana disease control ability is the best within the above range.

[0049] The organic substrate chelated to the above iodine (I) and sulfur (S) is preferably one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and methyl tolysulfon, and more preferably, methyl tolysulfon represented by the following chemical formula 1 can be used.

[0050] [Chemical Formula 1]

[0051]

[0052] Preferably, the composition for controlling banana disease further comprises organically passivated iodine (I), and the organically passivated iodine (I) may be organically passivated by chelating an organic substrate to the iodine (I).

[0053] The organic substrate chelated to the organic iodine (I) is preferably one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and propynyl butyl carbamate. More preferably, the organic substrate is butyl carbamate represented by the following chemical formula 2. In this case, the banana disease control ability is the best, and the control efficiency can be improved.

[0054] [Chemical Formula 2]

[0055]

[0056] The above composition for controlling banana diseases may additionally contain glycomacropeptide (GMP). In this case, it may be particularly more effective against banana virus diseases.

[0057] The above banana disease may be a fungal disease.

[0058] The fungi causing the above fungal diseases may be Fusarium oxysporum, Mycosphaerella fijiensis, Mycosphaerella musae, Mycosphaerella musicola, Cordana musae, Guignardia musae, Colletotrichum musae, Caldosporium musae, Verticillium sp., Chloridium spp., etc.

[0059] The above banana disease may be a bacterial disease.

[0060] The bacteria causing the above bacterial disease may be Erwinia sp., Pseudomonas sp., etc.

[0061] The above banana disease may be a viral disease.

[0062] The above viral diseases may be BBTV (Banana bunchy top virus), BBMV (Banana bract mosaic virus), CMV (Cucumber mosaic virus), BSV (Banana streak virus), etc.

[0063]

[0064] The organic passivation iodine(I)-sulfur(S) may preferably contain 25 to 60 wt% of the total weight of the iodine(I)-sulfur(S), and more preferably 35 to 55 wt%. If it is less than the minimum content, the effect of controlling banana disease may be reduced, and if it exceeds the maximum content, there is a concern that the organic passivation ratio by the chelating agent may be reduced, resulting in a loss of iodine(I)-sulfur(S).

[0065] Since the above organic iodine (I)-sulfur (S) is insoluble, the control efficiency may be greatly reduced when applied to plants in this state.

[0066] Therefore, it is desirable to make such insoluble organic minerals water-soluble and manufacture them into a formulation suitable for application to plant control.

[0067] The above organic passivation mineral is preferably prepared into a water-soluble formulation by mixing it with one solvent selected from butyldiol and polyethylene glycol, and more preferably, it can be prepared into a water-soluble formulation using polyethylene glycol.

[0068] The above water-soluble formulation is preferably mixed with the organic passivating mineral and the solvent in a weight ratio of 5:95 to 25:75, more preferably in a weight ratio of 10:90 to 20:80. When mixed in such a content ratio, the absorption rate in the body of banana plants increases, thereby improving the control efficiency.

[0069] Meanwhile, the composition for controlling banana disease of the present invention may optionally include additional components. Specifically, mineral components such as nitrogen, water-soluble phosphoric acid, water-soluble potassium, water-soluble kaolin, water-soluble boron, water-soluble iron, water-soluble molybdenum, water-soluble calcium, and water-soluble silicic acid may be included, and amino acids, lactoferrin, immunoglobulins A, D, E, and M, beta-lactoglobulin, alpha-lactalbumin, fat, lactose, and dextrin may be additionally included.

[0070] The above banana disease control composition can be used as a irrigation or foliar application, and it is preferable that the irrigation composition be manufactured in powder form, and the foliar application composition be manufactured in liquid form.

[0071] The above composition for controlling banana disease can be used as a preventive agent for preventing the occurrence of banana disease or as a management agent for suppressing symptoms after banana disease occurs.

[0072] The above composition for controlling banana disease may additionally contain an excipient.

[0073] It is preferable that the above excipient be non-toxic to microorganisms, applicable for agricultural use, not clog the nozzle of the sprayer, and capable of controlling moisture content.

[0074] Such excipients may include carbohydrates, polymers, lipids, and inorganic substances, and examples thereof include lactose, sucrose, mannitol, dextrin, cyclodextrin, bentonite, kaolin, zeolite, starch, cellulose ether, cellulose carboxymethylcellulose, alginate, carrageenan, hyaluronic acid, and polyacrylic acid, as long as they do not reduce the efficacy of the antifungal agent and do not cause any problems in use.

[0075] In addition, the composition for controlling banana disease according to one embodiment of the present invention may additionally include pesticide-acceptable buffers, diluents, and adjuvants known in the art.

[0076] The above buffer refers to an aqueous solution containing an acid-base mixture for the purpose of stabilizing pH, and examples thereof include tris, phosphate, carbonate, acetate, citrate, glycolate, lactate, borate, tartrate, cacodylate, ethanolamine, glycine, imidazole, imidazole lactic acid, etc.

[0077] The above diluent is mixed for the purpose of diluting the concentration of the formulation and may be saline solution, water, polyethylene glycol, propylene glycol, ethanol, or vegetable oil such as corn oil, peanut oil, cottonseed oil, or sesame oil.

[0078] The above adjuvant refers to a specific compound added to the formulation to increase the biological effect.

[0079] Meanwhile, the composition for controlling banana disease of the present invention can be manufactured in the form of tablets, aerosols, ointments, etc. in addition to the powder and liquid forms described above, and, if necessary, ingredients known in the art such as emulsifiers, suspending agents, spreading agents, penetrating agents, wetting agents, and stabilizers can be mixed therein.

[0080]

[0081] In addition, the present invention provides a method for controlling banana disease using a composition for controlling banana disease.

[0082] The banana disease control method of the present invention can be performed by diluting the banana disease control composition of the present invention to a predetermined concentration and performing foliar and / or irrigation treatment, or alternating treatment.

[0083] For fungal or bacterial diseases such as Panama disease of bananas, it is preferable to treat with a water-soluble agent containing organic passivating iodine (I)-sulfur (S) (which may additionally contain organic passivating iodine (I)) and the above solvent by diluting it 1500 to 2500 times for infection prevention through foliar and / or irrigation treatment, more preferably by diluting it 1800 to 2200 times, and most preferably by diluting it about 2000 times.

[0084] In addition, for fungal diseases or bacterial diseases such as Panama disease of banana, it is preferable to treat with a water-soluble agent containing organic passivating iodine(I)-sulfur(S) (which may additionally contain organic passivating iodine(I)) and the above solvent by diluting it 500 to 1500 times for growth inhibition as foliar and / or irrigation treatment, more preferably by diluting it 800 to 1200 times, and most preferably by diluting it about 1000 times. At this time, fungal diseases can exhibit a control effect of 99.999% with instantaneous contact, and bacterial diseases can exhibit a control effect of 99% or more within 30 minutes to 24 hours.

[0085] Meanwhile, it is preferable to treat banana virus disease with a water-soluble preparation containing organic passivated iodine(I)-sulfur(S) (which may further contain organic passivated iodine(I)) and the solvent alone, or with glycomacropeptide (GMP) together. In some cases, at this time, it is preferable to use the water-soluble preparation containing organic passivated iodine(I)-sulfur(S) after diluting it 1500 to 2500 times, more preferably, it can be treated after diluting it 1800 to 2200 times, and most preferably, it can be treated after diluting it about 2000 times. In addition, it is preferable to treat with glycomacropeptide (GMP) after diluting it 400 to 600 times based on a preparation containing 25 to 35 wt% of GMP, more preferably, it can be treated after diluting it 450 to 550 times, and most preferably, it can be treated after diluting it about 500 times. Organic iodine (I)-sulfur (S) and glycomacropeptide (GMP) can be used in combination, but it is preferable to use them alternately.

[0086] Additionally, the formulation containing 25 to 35 wt% of the GMP may additionally contain blood powder. The blood powder is preferably contained in an amount of 5 to 25 wt%, and more preferably in an amount of 10 to 14 wt%.

[0087] Meanwhile, the above composition for controlling banana disease may additionally include a coating suppression agent.

[0088] Hereinafter, preferred examples are presented to help understand the present invention, but the following examples are only illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and technical idea of ​​the present invention, and it is also natural that such changes and modifications fall within the scope of the appended patent claims.

[0089]

[0090] [Example]

[0091] Manufacturing Example 1: Organically passivated iodine (I) water-soluble preparation

[0092] As a substrate for chelation, propynyl butyl carbamate and iodine (I2) were mixed in a weight ratio of 5:2, added to purified water, stirred at 5000 rpm at room temperature to dissolve, heat-treated at 95℃ for 30 minutes while stirring at 200 rpm, transferred to a storage tank, cooled to room temperature, centrifuged to remove the supernatant, added purified water to the precipitate again, stirred, and centrifuged again under the same conditions. This washing process was repeated four times, and then spray-dried using a high-temperature, short-time sterilization method to prepare an insoluble organic passivated iodine (I) raw material. Thereafter, the insoluble organic passivated iodine (I) raw material and PEG (poly ethylen glycol) solvent were mixed in a weight ratio of 1:9 to prepare an organic passivated iodine (I) water-soluble formulation.

[0093]

[0094] Manufacturing Example 2: Organically passivated iodine-sulfur (IS) water-soluble formulation

[0095] Using methyl tolysulfone as a substrate for chelation, an insoluble organic passivation iodine-sulfur (IS) raw material containing iodine (I):sulfur (S) in a weight ratio of 33:4 was prepared in the same manner as in Manufacturing Example 1. Thereafter, the insoluble organic passivation iodine-sulfur (IS) raw material and PEG (poly ethylen glycol) solvent were mixed in a weight ratio of 1:9 to prepare an organic passivation iodine-sulfur (IS) water-soluble formulation.

[0096]

[0097] Manufacturing Example 3: GMP containing sialic acid

[0098] (1) Preprocessing

[0099] 400 kg of RO water was added to a 20 L reactor and the temperature was raised to 57±2°C while stirring. Then, to achieve the optimal pH composition for the enzyme reaction, 4 kg of NaOH (98%) was added once to achieve a pH of 12±0.5 and a temperature of 58±2°C. Next, 400 kg of whey was added and stirred for 30 minutes. At this time, the final pH was adjusted to 8.50±0.3 and the temperature to 47±2°C.

[0100] (2) Enzyme decomposition

[0101] While maintaining 47±2℃, 2800ml of lactose was added to the reactor to perform enzymatic digestion, then 80ml of lipase was added to perform enzymatic digestion, and 600g of protease was added to perform enzymatic digestion sequentially, and the mixture was stirred.

[0102] (3) Enzyme inactivation

[0103] The reactor temperature was raised to 76±2℃, and the reactant became gel-like. To further confirm enzyme inactivation, 12 kg of NaOH (98%) was added four times at 5-minute intervals and stirred. The pH gradually decreased, the color became a deep coffee color, and the reaction was terminated when the pH reached 7 or lower.

[0104] (4) Powdering

[0105] Glycomacropeptide (GMP) was obtained by powdering in a spray dryer (containing 0.13 g of sialic acid per 25 g of GMP).

[0106]

[0107] Example 1: Organically passivated iodine (I) and organically passivated iodine-sulfur (IS) mixed preparation (Mineral Preparation 1006)

[0108] Mineral preparation 1006 was prepared by mixing the organically passivated iodine (I) water-soluble preparation of Manufacturing Example 1 and the organically passivated iodine-sulfur (IS) water-soluble preparation of Manufacturing Example 2 in a weight ratio of 10:6.

[0109]

[0110] Example 2: Organically passivated iodine (I) and organically passivated iodine-sulfur (IS) mixed preparation (Mineral Preparation 1020)

[0111] Mineral preparation 1020 was prepared by mixing the organically passivated iodine (I) water-soluble preparation of Manufacturing Example 1 and the organically passivated iodine-sulfur (IS) water-soluble preparation of Manufacturing Example 2 in a weight ratio of 10:20.

[0112]

[0113] Example 3: Organically passivated iodine (I) and organically passivated iodine-sulfur (IS) mixed preparation (Mineral Preparation 1012)

[0114] Mineral preparation 1012 was prepared by mixing the organically passivated iodine (I) water-soluble preparation of Manufacturing Example 1 and the organically passivated iodine-sulfur (IS) water-soluble preparation of Manufacturing Example 2 in a weight ratio of 10:12.

[0115]

[0116] Example 4: Organically passivated iodine-sulfur (IS) and GMP complex formulation

[0117] A GMP mixed preparation was prepared by mixing 17.5 g of glycomacropeptide (GMP) of Manufacturing Example 3 (including 0.09 g of sialic acid), 10.925 g of the organically passivated iodine (I)-sulfur (S) insoluble preparation prepared in Manufacturing Example 2 (including 3.3 g of I and 0.4 g of S), 7.5 g of blood meal (including 1.07 g of N), and 17.5 g of excipient (dextrin).

[0118]

[0119] [Experimental Example]

[0120] Isolation of banana disease-causing microorganisms

[0121] Bacteria were collected from the vascular part of the banana plant where the disease had occurred, and molds were collected from the surface and cultured on TSA medium. The photographs thereof are shown in Fig. 1. According to this, the white type in Fig. 1 (a) is a bacterium of the genus Erwinia, the yellow type is a bacterium of the genus Pseudomonas, and (b) is Fusarium oxysporum.

[0122] Figure 2 shows the results of pure isolation of white-type Erwinia sp. (a) and yellow-type Pseudomonas sp. (b) bacteria, respectively.

[0123]

[0124] Experimental Example 1: Analysis of Banana Disease Control Effects

[0125] A mixed strain (2x10) containing the fungus Fusarium oxysporum isolated from the surface of the banana and the bacteria including the white type Erwinia. sp. and the yellow type Pseudomonas sp. isolated from the vascular part of the banana, in a ratio of about 1:1 9cfu / ㎖) of the mineral preparation 1006 of Example 1, the mineral preparation 1020 of Example 2, and the mineral preparation 2012 of Example 3 were treated at different concentrations for 24 hours, and the photos of the results are shown in Fig. 3. According to this, when the mixed strains were treated with the mineral preparations according to the examples, the fungus Fusarium oxysporum was all controlled by instantaneous contact, and bacteria including the white type Erwinia. sp. and the yellow type Pseudomonas sp. were controlled for 30 to 48 hours. In addition, when the mineral preparation 1006 of Example 1 was treated at a high concentration of 100 times dilution, and when the mineral preparation 2012 of Example 3 was treated at 100 times and 500 times dilution, control was achieved by more than 99% after 24 hours. The above dilution ratio is expressed as a volume ratio.

[0126] In addition, the results of the control effect over time after treatment of the above mixed strain with the mineral preparation 1006 of Example 1 and the mineral preparation 2012 of Example 3 at different concentrations are shown in Fig. 4. According to this, in the mineral preparation 2012 treatment group of Example 1, it was shown that control was achieved by more than 99% in the 100-fold and 500-fold dilution treatment groups after 24 hours of treatment. In addition, the mineral preparation 2012 treatment group of Example 3 showed control of more than 99% even after 3 hours when treated with a high concentration of 100-fold dilution.

[0127] Meanwhile, the white Erwinia genus (Erwinia. sp.) strain was treated with mineral preparation 1006 of Example 1 and mineral preparation 1020 of Example 2 at various concentrations for 24 hours, and the photos of the results are shown in Fig. 5. According to this, the mineral preparation 1006 treatment group of Example 1 showed a control rate of 99% or more in the 100-fold and 500-fold dilution treatment groups, and the mineral preparation 1020 treatment group of Example 2 showed a control rate of 99% or more in the 100-fold and 500-fold dilution treatment groups, and also showed a control rate of 80% or more in the 1000-fold dilution treatment group.

[0128] Meanwhile, the mineral preparation 1006 of Example 1 and the mineral preparation 2012 of Example 3 were treated at various concentrations for 24 hours against the yellow Pseudomonas sp. strain, and the photos of the results are shown in Fig. 6. According to this, the mineral preparation 1006 treatment group of Example 1 showed a control rate of over 99% in the 100-fold and 500-fold dilution treatment groups, and also showed a control rate of over about 80% in the 1000-fold dilution treatment group. In addition, it was confirmed that the mineral preparation 2012 treatment group of Example 3 showed a control rate of over 99% in all of the 100-fold, 500-fold, and 1000-fold dilution treatment groups.

[0129] Meanwhile, in order to investigate the instantaneous contact control effect on the Fusarium oxysporum fungus isolated from the field, mineral preparation 1006 of Example 1 and mineral preparation 1020 of Example 2 were treated, and the results are shown in Fig. 7. According to this, both the mineral preparation treatment groups of Example 1 and Example 3 showed an instantaneous contact control effect of 99.999% or more based on the 1000-fold dilution treatment group.

[0130]

[0131] Experimental Example 2: Evaluation of resistance and suppression ability against Panama disease in bananas.

[0132] An evaluation of the resistance of Erwinia. sp. and Pseudomonas sp. bacteria to Fusarium oxysporum, a fungus causing Panama disease of bananas isolated from the field, and an evaluation of the inhibitory activity of mineral preparation 1006 of Example 1 and mineral preparation 1020 of Example 2 against Fusarium oxysporum were performed, and the results are shown in Table 1 below. Here, the evaluations were indicated as (-) no effect, (+) slightly effective, (++) moderately effective, and (+++) very effective.

[0133] Bacterial resistance evaluation results Fusarium oxysporum (untreated group) - Fusarium oxysporum vs. Erwinia - Fusarium oxysporum vs. Pseudomonas - Fusarium oxysporum vs. Erwinia + Pseudomonas - Inhibitory activity results of mineral preparations Preparation of Example 1 vs. Fusarium oxysporum 1006+++ Preparation of Example 2 vs. Fusarium oxysporum 1020+++

[0134] Fig. 8 is the final result of evaluating the resistance of bacteria or mixed bacteria and the inhibitory ability of mineral preparations compared to the untreated group, and Fig. 9 shows the trend of the resistance of bacteria or mixed bacteria and the inhibitory ability of mineral preparations by date. Here, the treated portions of mineral preparation 1006 of Example 1 and mineral preparation 1020 of Example 2 were treated with lines (dotted lines) above and below the Fusarium oxysporum fungus. According to the results of the bacterial resistance evaluation, the resistance of Erwinia. sp. bacteria and Pseudomonas sp. bacteria to the Fusarium oxysporum fungus did not show resistance either when measured separately or as a mixed strain.

[0135] In addition, according to the results of the evaluation of the inhibitory ability of the mineral preparations of Example 1 or 2 against the fungus Fusarium oxysporum, both the mineral preparation 1006 of Example 1 and the mineral preparation 1020 of Example 2 showed excellent Fusarium oxysporum growth inhibitory ability.

[0136]

[0137] Experimental Example 3: Analysis of Erwinia amylovora growth inhibition and control activity.

[0138] Erwinia amylovora is a Gram-negative bacterium that is 0.6 to 2.5 ㎛ long and rod-shaped with 2 to 7 flagella. It secretes bacterial ooze, a sugar substance such as glucan, which blocks the xylem and phloem of plants, causing wilting symptoms and fire blight symptoms.

[0139] In this experimental example, the results of an experiment on the growth inhibition effect of mineral preparation 1006 of Example 1 on the Erwinia amylovora strain causing fire blight at different dilution concentrations are shown in Fig. 10, and the results of an experiment on the growth inhibition effect of mineral preparation 1020 of Example 2 at different dilution concentrations are shown in Fig. 11.

[0140] According to the results of Fig. 10, when treated with mineral preparation 1006 of Example 1, the growth of Erwinia amylovora was almost non-existent for up to 24 hours, and even when treated with a 5000-fold dilution, the growth rate was found to be very low compared to the control group after 24 hours.

[0141] In addition, according to the results of FIG. 11, the mineral preparation 1020 of Example 2 hardly caused the growth of Erwinia amylovora for up to 24 hours in all cases of 500-fold, 1000-fold, and 5000-fold dilution treatments.

[0142] Figure 12 shows the Erwinia amylovora strain (number of bacteria 2x10) according to the instantaneous or long-term contact with the mineral preparation 1006 of Example 1 at different concentrations. 9 This is the result showing the control effect against (cfu / ml). According to this, when the mineral preparation 1006 of Example 1 was diluted 500 to 1000 times, it was found that more than 99% of the bacteria were killed within 1 minute by instantaneous contact.

[0143]

[0144] Experimental Example 4: Analysis of Banana Virus Disease Control Efficacy

[0145] Figure 13 is a photograph of a banana infected with BBTV (Banana bunchy top virus). In order to evaluate the virus inhibitory ability against BBTV, a 2000-fold dilution of the mineral preparation 1006 of Example 1 and a 500-fold dilution of the organic passivated iodine-sulfur (IS) / GMP complex preparation of Example 4 were alternately applied, and a control value of 60% or more was observed.

[0146]

[0147] Experimental Example 5: Field Evaluation I (Control Effect in Infected Fields)

[0148] Bananas were grown in an existing cultivation area infected with Panama disease caused by the fungus Fusarium oxysporum (13 tons of bananas harvested per ha using organic fertilizer, infection rate 40 to 90%), and bananas were grown while controlling the disease using the organic passivating iodine-sulfur (IS) and GMP complex preparation of Example 4, and the process is as follows.

[0149] (1) 2023.04.21: 25 ha of existing infected cultivation area were secured and infected banana seedlings were transplanted at the bract formation stage.

[0150] (2) 2023.05.19: A photo of the cultivation site 4 weeks after planting is shown in Fig. 14.

[0151] (3) 2023.06.14: The complex preparation of Example 4 was diluted 2000 times and treated for the first time.

[0152] (4) 2023.06.24: A photograph taken 10 days after treatment with the compound preparation of Example 4 is shown in Fig. 15. According to this, it can be seen that bananas were grown well without any disease symptoms.

[0153] (5) 2023.07.15: The compound preparation of Example 4 was administered for the second time.

[0154] (6) 2023.07.25: A photograph taken 10 days after the second treatment with the compound preparation of Example 4 is shown in Fig. 16. According to this, it can be seen that bananas are being grown without any disease symptoms.

[0155]

[0156] (7) 2023.08.08: The photograph taken 25 days after the second treatment with the compound preparation of Example 4 is shown in Fig. 17. According to this, the bananas grew without any disease symptoms up to a stem height of approximately 85 cm (Special note: overcame damage from 7 days of rainy season and flooding).

[0157] (8) 2023.08.28: A photograph taken 45 days after the second treatment with the compound preparation of Example 4 is shown in Fig. 18. According to this, the bananas grew well without any disease symptoms up to a stem height of approximately 160 cm.

[0158] (9) 2023.10.12: Bananas were harvested.

[0159] At this time, a photograph comparing the banana harvest area and the control farm that was not treated with the compound preparation of Example 4 is shown in Figure 19. According to this, 5,000 banana plants in the control farm of 25 ha had a banana Panama disease infection rate of over 70%, making it impossible to control and leading to closure. However, in the case of the compound preparation treatment area of ​​Example 4, the cumulative incidence of Panama disease over a total of 30 weeks was 0.06%, showing a very high Panama disease control effect.

[0160] In addition, a photograph of a banana at harvest is shown in Fig. 20. According to this, the banana stem height at harvest was approximately 170 cm, which is shorter than the typical banana that grows to over 280 cm. In other words, the composite preparation of Example 4 of the present invention was found to have a panama disease control effect as well as a banana leaf suppression effect by suppressing banana height growth.

[0161]

[0162] Experimental Example 6: Field Evaluation II (Control Effect in New Fields)

[0163] Before transplanting banana seedlings on 25 ha of new land not infected with Panama disease, the plants were grown by soaking them in a complex formulation of 4, and the process is as follows.

[0164] (1) 2023.08.22: The infection rate of Panama disease was 40 to 90% at the seedling stage. Before transplanting, the seedlings were soaked in a 2000-fold diluted compound of Example 4 and then transplanted to the cultivation site. A photograph of the bananas that had gone through the seedling stage is shown in Fig. 21.

[0165] (2) 2023.09.09: A comparison of a photo taken 18 days after planting and a conventional cultivation area that was not treated with the compound preparation of Example 4 is shown in Fig. 22. According to this, in the compound preparation treatment area of ​​Example 4, the root activation effect was 200% or more, the growth effect was 300% or more, and the disease control effect such as root rot was 99% or more.

[0166] (3) September 22, 2023: Damage occurred due to the monsoon season. Nevertheless, the compound treatment of Example 4 demonstrated a 100% root rot control effect, enabling recovery from flood damage. In contrast, Panama disease occurred in over 50% of the control plots.

[0167] (4) 2023.10.12: A photograph comparing the condition of bananas after about two months with the control group is shown in Fig. 23. According to this, in the compound treatment group of Example 4, Panama disease did not occur at all in the 30,000 plants planted, while in the control group, the Panama disease infection rate was over 70%, leading to the arable land being closed as control was impossible.

[0168] The composition for controlling banana diseases comprising organic passivating iodine and sulfur of the present invention as effective ingredients can effectively control banana diseases caused by fungi, including Panama disease, and banana diseases caused by bacteria or viruses caused by Gram-negative bacteria.

Claims

1. A composition for controlling banana disease, comprising organically passivated iodine (I)-sulfur (S) as an effective ingredient, wherein the organically passivated iodine (I)-sulfur (S) is characterized in that an organic substrate is organically passivated by chelating with the iodine (I) and sulfur (S).

2. In paragraph 1, A composition for controlling banana disease, characterized in that the weight ratio of iodine (I) and sulfur (S) in the organic iodine (I)-sulfur (S) is 10:0.5 to 10:

2.

3. In paragraph 1, A composition for controlling banana disease, characterized in that the organic substrate chelated to the iodine (I) and sulfur (S) is any one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and methyl tolysulfon.

4. In paragraph 1, The composition for controlling the above banana disease further comprises organically passivated iodine (I), wherein the organically passivated iodine (I) is characterized in that an organic substrate is organically passivated by chelating with the iodine (I).

5. In paragraph 4, A composition for controlling banana disease, characterized in that the organic substrate chelated to the organic iodine (I) is any one selected from milk casein, chitosan, alginic acid, starch, blood protein, vitamin C, and propynyl butyl carbamate.

6. In paragraph 1, A composition for controlling banana disease, characterized in that the organic iodine (I)-sulfur (S) is prepared into a water-soluble formulation by mixing it with any one solvent selected from butyldiol and polyethylene glycol.

7. In paragraph 1, A composition for controlling banana disease, characterized in that the composition for controlling banana disease additionally contains glycomacropeptide (GMP).

8. In paragraph 1, A composition for controlling banana disease, characterized in that the above banana disease is a fungal disease, and the fungus causing the fungal disease is any one selected from among Fusarium oxysporum, Mycosphaerella fijiensis, Mycosphaerella musae, Mycosphaerella musicola, Cordana musae, Guignardia musae, Colletotrichum musae, Caldosporium musae, Verticillium sp., and Chloridium spp.

9. In paragraph 1, A composition for controlling banana disease, characterized in that the above banana disease is a bacterial disease, and the bacteria causing the bacterial disease are any one selected from Erwinia sp. and Pseudomonas sp.

10. In paragraph 1, A composition for controlling banana disease, characterized in that the above banana disease is a viral disease, and the virus causing the viral disease is any one selected from BBTV (Banana bunchy top virus), BBMV (Banana bract mosaic virus), CMV (Cucumber mosaic virus), and BSV (Banana streak virus).

11. In paragraph 1, A composition for controlling banana disease, characterized in that the composition for controlling banana disease additionally contains a paint suppression agent.

12. A method for controlling banana disease using a composition for controlling banana disease selected from any one of claims 1 to 11.

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