Method for predicting the efficacy of an antibody-drug-conjugate (ADC) using multiplex fluorescence in situ hybridization (FISH) and multiplex sequencing

The method integrates multiplex FISH and sequencing to accurately predict the efficacy of ADCs in breast cancer by determining the presence of specific biomarkers, addressing the limitations of current single-technology approaches.

WO2025114609A1PCT designated stage expired Publication Date: 2025-06-05MULTIPLEXDX SRO
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Patent Information

Application Number
PCT/EP2024/084353
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-12-01
Filing Date
2024-12-02
Publication Date
2025-06-05

AI Technical Summary

Technical Problem

Current methods for predicting the efficacy of antibody-drug-conjugates (ADCs) in breast cancer treatment are imprecise, relying on single technologies and failing to provide accurate data for selecting the most effective treatment.

Method used

A method combining multiplex fluorescence in situ hybridization (FISH) and multiplex sequencing to determine the presence of ADC-associated biomarkers in breast cancer samples, allowing for precise prediction of ADC efficacy.

Benefits of technology

This method enhances the accuracy of predicting ADC efficacy by providing spatial localization and quantitative analysis of biomarkers, thereby minimizing false positives and negatives and improving treatment stratification.

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Abstract

The present invention relates to methods for predicting the efficacy of an antibody-drug- conjugate (ADC) comprising determining by multiplex fluorescence in situ hybridization whether or not mRNA species of ADC-associated biomarker / s are present in a sample and determining by multiplex sequencing whether or not mRNA species of ADC-associated biomarkers are present in said sample. The present invention also relates to kits for performing the methods for predicting the efficacy of an antibody-drug-conjugate (ADC) as described and provided herein as well as use of such kits for performing the methods as described and provided herein.
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Citation Information

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