Multi-effect composition containing recombinant collagen, preparation method therefor, and use thereof

Abstract

The present application provides a multi-effect composition containing recombinant collagen, a preparation method therefor, and use thereof. The multi-effect composition comprises the recombinant collagen, Theobroma grandiflorum fruit nanocapsules, supramolecular Bombax ceiba flowers, and supramolecular carnosine. In the multi-effect composition provided by the present application, the use of the Theobroma grandiflorum fruit nanocapsules effectively improves the bioavailability of functional active ingredients, and the supramolecular carnosine can protect collagen and elastin from being damaged by oxidative stress, thereby maintaining a good skin structure and offering an anti-glycation effect. The use of the supramolecular Bombax ceiba flowers, which have an anti-oxidation effect, in combination with the supramolecular carnosine, the collagen, and a Theobroma grandiflorum fruit extract results in a synergy that multi-effectively resolves skin problems, and the moisturizing effect is further improved while an anti-oxidation effect, a skin regeneration promoting effect, and an anti-glycation effect are achieved.

Description

A multi-effect composition containing recombinant collagen and its preparation method and application Technical Field

[0001] The present application belongs to the technical field of skin care products, and specifically relates to a multi-effect composition containing recombinant collagen, and a preparation method and application thereof. Background Art

[0002] As we age, our bodily functions decline, causing the skin's epidermis to dry out and thin. Collagen and elastin are gradually lost, damaging the fine mesh structure formed by the interwoven collagen and elastic fibers. This damages the fine, coarse, and fine wrinkles that appear. In addition to the natural aging of the skin itself, many environmental factors can accelerate the aging process, such as excessive sugar intake, ultraviolet radiation, and unhealthy lifestyles. Given these factors influencing skin aging, active ingredients with anti-glycation, antioxidant, anti-photoaging, and skin repair properties are crucial for combating skin aging.

[0003] Collagen has the ability to stretch, maintain skin elasticity and promote cell growth. When used in skin care products, it can have moisturizing, repairing, and anti-wrinkle effects. It also has good biodegradability and bio-cosmetic properties. Therefore, collagen is also beginning to be used more and more in skin care products.

[0004] Theobroma cacao, scientifically known as theobroma cacao, is a fruit that grows in the Amazon rainforest in northern Brazil. Its fruit is rich in vitamins, fiber, and amino acids, making it a natural antioxidant. A natural, buttery oil can be extracted from the seeds. This oil is rich in vitamins (especially vitamins A and C), minerals, amino acids, fatty acids, and flavonoids, and is extremely beneficial to the human body. Long-term application to the skin leaves it delicate, hydrated, silky, and radiant. It also has excellent moisturizing and skin-repair properties, is highly resistant to UV rays, and is non-irritating and safe. It effectively absorbs ultraviolet rays from sunlight, helping to prevent sunburn and reduce post-sun redness. It is a rare food ingredient and natural skincare product for locals.

[0005] Kapok flower extract is rich in triterpenes and flavonoids, which can scavenger free radicals and promote the synthesis of aquaporin 3 and hyaluronic acid, rapidly replenishing moisture to skin cells, improving skin elasticity, and repairing fine lines. Kapok flower, a traditional Chinese medicine ingredient, is sweet and cooling in nature, and has the effects of clearing heat and dampness, detoxifying, and stopping bleeding. Topical application has certain anti-inflammatory and repairing effects.

[0006] CN102836093A discloses a facial cream containing type I collagen, which comprises the following components by mass percentage: 3-8% glycerol, 1-3% trehalose, 0.1-0.3% methylparaben, 0.05-0.2% propylparaben, 0.1-0.5% allantoin, 0.5-2% VC ethyl ether, 0.5%-2% vitamin B3, 0.5-1% dipotassium glycyrrhizate, 1-2% M68, 1-10% A165, 1-2% 16-18 alcohol, and DC200. 1-2%, GTCC2-10%, Shea Butter1.5-10%, Cupuacu Butter3-6%, 2EHP1-5%, Vitamin A Acetate 0.1-1%, Vitamin E Acetate 0.4-1%, Piperine 0.01-0.1%, Type I Collagen 0.5-2%, Fragrance 0.01-0.05%, S-305 0.1-0.5%, LS8740 1-5%; it has the effect of increasing skin firmness, reducing deep wrinkles, smoothing fine lines, making skin firm and elastic, and can improve skin elasticity from deep layers, with obvious moisturizing, hydrating and anti-aging effects.

[0007] CN106109277A provides a moisturizing composition comprising an amino acid moisturizer, cupuaçu fruit oil, and an adjuvant. The cupuaçu fruit oil content is limited so that it can fully exert its moisturizing activity and synergize with the amino acid moisturizer to achieve a better moisturizing effect.

[0008] CN113081874A discloses that kapok extract and decarboxylated carnosine are added to different cosmetic preparations to provide personalized beauty treatments for different skin types.

[0009] However, in the prior art, cupuacu fruit extract and kapok flower extract still have problems such as slow onset of action and unclear effect due to low skin bioavailability.

[0010] Therefore, developing a composition that can combine collagen with cupuacu fruit extract and kapok flower extract to achieve multiple effects such as promoting skin repair and regeneration, anti-photoaging, anti-oxidation, and anti-glycation is a research focus in this field.

[0011] Summary of the Invention

[0012] The following is a summary of the subject matter described in detail herein. This summary is not intended to limit the scope of the claims.

[0013] In view of the shortcomings of the existing technology, the purpose of this application is to provide a multi-functional composition containing recombinant collagen and its preparation method and application, which has multiple functions such as promoting skin repair and regeneration, anti-photoaging, anti-oxidation, and anti-glycation.

[0014] To achieve this goal, this application adopts the following technical solutions:

[0015] In a first aspect, the present application provides a multi-functional composition containing recombinant collagen, wherein the multi-functional composition comprises recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers, and supramolecular carnosine.

[0016] The cupuacu fruit in the multi-effect composition provided by the present application has multiple functions such as promoting skin regeneration, anti-oxidation, and anti-photoaging. The use of cupuacu fruit nanocapsules effectively improves the bioavailability of functional active ingredients; supramolecular carnosine can protect collagen and elastin from damage by oxidative stress, thereby maintaining a good skin structure. The addition of supramolecular kapok flowers with antioxidant effects and supramolecular carnosine with anti-glycation effects can synergize with collagen and cupuacu fruit extracts, further improving the hydrating and moisturizing effects while providing anti-oxidation.

[0017] In one embodiment, the multi-effect composition includes, by weight, 0.1-0.5 parts of recombinant collagen, for example, 0.2 parts, 0.3 parts, 0.4 parts, etc., 1-10 parts of cupuacu fruit nanocapsules, for example, 2 parts, 4 parts, 6 parts, 8 parts, etc., 1-10 parts of supramolecular kapok flowers, for example, 2 parts, 4 parts, 6 parts, 8 parts, etc., and 0.1-1 parts of supramolecular carnosine, for example, 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, etc.

[0018] In one embodiment, the cupuacu fruit nanocapsules are prepared by the following method, which comprises:

[0019] (1) dissolving cupuacu oil and pluronic in acetone to obtain a mixed solution;

[0020] (2) dropping the mixed solution into distilled water that is being stirred, and continuing to stir, and removing acetone by natural evaporation to obtain nanoparticles;

[0021] (3) adding chitosan to the continuously stirred nanoparticles to obtain the cupuacu fruit nanocapsules.

[0022] In one embodiment, the hydrophilic-lipophilic balance value of the Pluronic is 8-29, for example, it can be 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, etc.

[0023] In one embodiment, the molecular weight of the chitosan is 3-20 kDa, for example, 4 kDa, 6 kDa, 8 kDa, 10 kDa, 12 kDa, 14 kDa, 16 kDa, 18 kDa, etc.

[0024] In one embodiment, the mass ratio of the cupuacu oil to pluronic is 1:(3-5), for example, 1:3.5, 1:4, 1:4.5, etc.

[0025] In one embodiment, the mass volume ratio of the cupuaçu oil and acetone is (4-6) mg:1 mL, for example, 4.5 mg:1 mL, 5 mg:1 mL, 5.5 mg:1 mL, etc.

[0026] In one embodiment, the amount of distilled water used is 4 times that of acetone.

[0027] In one embodiment, the particle size of the cupuacu fruit nanocapsules is 30-60 nm, for example, 35 nm, 40 nm, 45 nm, 50 nm, 55 nm, etc.

[0028] In one embodiment, the supramolecular kapok flower is prepared by the following method, which comprises: extracting kapok flower powder by using a DES-TG aqueous solution to obtain the supramolecular kapok flower.

[0029] In one embodiment, the material-liquid ratio of the kapok flower powder to the DES-TG aqueous solution is 1:(30-70), for example, 1:40, 1:50, 1:60, etc.

[0030] In one embodiment, the DES-TG aqueous solution includes betaine, glycerol and propylene glycol.

[0031] In a second aspect, the present application provides a method for preparing the multi-effect composition containing recombinant collagen as described in the first aspect, the preparation method comprising: uniformly mixing recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers and supramolecular carnosine to obtain the composition.

[0032] In a third aspect, the present application provides an antioxidant moisturizing lotion, the raw materials of which include: the multi-effect composition containing recombinant collagen described in the first aspect, an emollient, a moisturizer, an emulsifier and a thickener.

[0033] In one embodiment, the mass percentage of the multi-effect composition in the antioxidant moisturizing emulsion is 1-10%, for example, 2%, 4%, 6%, 8%, 10%, etc.

[0034] The numerical range described in this application includes not only the point values ​​listed above, but also any point values ​​between the above numerical ranges that are not listed. Due to limited space and for the sake of simplicity, this application no longer exhaustively lists the specific point values ​​included in the range.

[0035] Compared with the prior art, this application has the following beneficial effects:

[0036] The multi-effect composition provided in the present application uses cupuacu fruit nanocapsules to effectively improve the bioavailability of functional active ingredients; supramolecular carnosine can protect collagen and elastin from damage by oxidative stress, thereby maintaining a good skin structure and having an anti-glycation effect; the supramolecular kapok flower with antioxidant effect is used in combination with supramolecular carnosine, collagen, and cupuacu fruit extract to synergistically solve skin problems with multiple effects, further improving the hydrating and moisturizing effects while resisting oxidation, promoting skin regeneration, and resisting glycation.

[0037] Still other aspects will become apparent upon reading and understanding the detailed description. DETAILED DESCRIPTION

[0038] The technical solution of the present application is further described below through specific implementation methods. Those skilled in the art should understand that the embodiments are only used to help understand the present application and should not be regarded as specific limitations of the present application.

[0039] As used herein, the terms "comprises," "including," "having," "containing" or any other variations thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a listed element is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0040] "Optional" or "either" means that the subsequently described event or circumstance can or cannot occur, and that the description includes instances where the event occurs and instances where it does not.

[0041] The indefinite articles "a" and "an" before an element or component of the present application do not limit the quantity requirement (i.e., the number of times the element or component appears). Therefore, "a" or "an" should be interpreted as including one or at least one, and elements or components in the singular also include plural forms, unless the quantity clearly refers to only the singular form.

[0042] The terms "one embodiment," "some embodiments," "exemplarily," "specific examples," or "some examples" used herein mean that a specific feature, structure, material, or characteristic described in conjunction with the embodiment or example is included in at least one embodiment or example of the present application. In this document, the schematic descriptions of the above terms do not necessarily refer to the same embodiment or example.

[0043] In this application, unless otherwise specified, percentages and contents are all by mass. Unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used can be purchased from commercial sources.

[0044] Recombinant collagen: Qidian Biotechnology, fully human TM Collagen, molecular weight 20-40KD;

[0045] Cupuaçu Butter: Clariant, Refined Butter;

[0046] Kapok: Best Pharmaceutical Company, Kapok;

[0047] Carnosine: Symrise, Dragosine.

[0048] Preparation Example 1

[0049] This preparation example provides a cupuacu fruit nanocapsule, and the preparation method is as follows:

[0050] (1) Dissolve 5 mg of cupuacu fruit oil and 20 mg of Pluronic P85 in 1 mL of acetone to obtain a mixture;

[0051] (2) the mixture was dropped into 4 mL of distilled water stirred at 400 rpm and stirred for 7 h. The acetone was removed by natural evaporation to obtain nanoparticles;

[0052] (3) 20 mg of chitosan (molecular weight 15 kDa) was added to the continuously stirred nanoparticles to obtain cupuacu fruit nanocapsules (particle size 40 nm).

[0053] Comparative Preparation Example 1

[0054] This comparative preparation example provides a cupuacu fruit nanocapsule, which differs from the preparation example only in that acetone is replaced by an equal amount of ethanol, and other raw materials, amounts and preparation methods are the same as those in Preparation Example 1.

[0055] Preparation Example 2

[0056] This preparation example provides a supramolecular kapok flower, and the preparation method is as follows:

[0057] (1) Dried kapok flowers were crushed and passed through a 30-mesh sieve to obtain a powder. Betaine, glycerol, and propylene glycol were added to water in a mass ratio of 20:60:3 to prepare a 40% supramolecular extract;

[0058] (2) Take 10 g of kapok flower powder and add kapok flowers at a ratio of 1 g of kapok flower powder to 40 mL of supramolecular extract. Perform high-voltage pulse extraction at a voltage of 600 V and 80 pulses. After extraction, filter the mixture to obtain supramolecular kapok flowers.

[0059] Preparation Example 3

[0060] This preparation example provides a supramolecular carnosine, which is prepared with reference to Example 2 in CN114306113A.

[0061] Example 1

[0062] This embodiment provides a multi-effect composition, comprising:

[0063] 0.3 parts of recombinant collagen, 8 parts of the cupuacu fruit nanocapsules of Preparation Example 1, 8 parts of the supramolecular kapok flowers of Preparation Example 2, and 0.3 parts of the supramolecular carnosine of Preparation Example 3; the above raw materials are mixed uniformly according to the formula to obtain a multi-effect composition.

[0064] Example 2

[0065] This embodiment provides a multi-effect composition, comprising:

[0066] 0.5 parts of recombinant collagen, 6 parts of the cupuacu fruit nanocapsules of Preparation Example 1, 10 parts of the supramolecular kapok flowers of Preparation Example 2, and 0.1 parts of the supramolecular carnosine of Preparation Example 3; the above raw materials are mixed uniformly according to the formula to obtain a multi-effect composition.

[0067] Example 3

[0068] This embodiment provides a multi-effect composition, comprising:

[0069] 0.1 parts of recombinant collagen, 10 parts of the cupuacu fruit nanocapsules of Preparation Example 1, 6 parts of the supramolecular kapok flowers of Preparation Example 2, and 0.5 parts of the supramolecular carnosine of Preparation Example 3; the above raw materials are mixed uniformly according to the formula to obtain a multi-effect composition.

[0070] Example 4

[0071] This embodiment provides a multi-effect composition, comprising:

[0072] 0.3 parts of recombinant collagen, 3 parts of the cupuacu fruit nanocapsules of Preparation Example 1, 2 parts of the supramolecular kapok flowers of Preparation Example 2, and 0.9 parts of the supramolecular carnosine of Preparation Example 3; the above raw materials are mixed uniformly according to the formula amount to obtain a multi-effect composition.

[0073] Example 5

[0074] This embodiment provides a multi-effect composition, comprising:

[0075] 0.3 parts of recombinant collagen, 12 parts of the cupuacu fruit nanocapsules of Preparation Example 1, 12 parts of the supramolecular kapok flowers of Preparation Example 2, and 2 parts of the supramolecular carnosine of Preparation Example 3; the above raw materials are mixed uniformly according to the formula to obtain a multi-effect composition.

[0076] Example 6

[0077] This embodiment provides a multi-effect composition, which differs from Example 1 only in that the cupuacu fruit nanocapsules in Preparation Example 1 are adjusted to an equal amount of the cupuacu fruit nanocapsules in Comparative Preparation Example 1, and other raw materials, amounts and preparation methods are referred to Example 1.

[0078] Comparative Example 1

[0079] This comparative example provides a multi-effect composition, which differs from Example 1 only in that 8 parts of the cupuacu fruit nanocapsules of Preparation Example 1 are replaced by 8 parts of cupuacu fruit oil. Other raw materials, amounts and preparation methods refer to Example 1.

[0080] Comparative Example 2

[0081] This comparative example provides a multi-effect composition, which differs from Example 1 only in that 8 parts of the supramolecular kapok flowers of Preparation Example 2 are replaced by 8 parts of kapok flower extracts. Other raw materials, amounts and preparation methods refer to those of Example 1.

[0082] Comparative Example 3

[0083] This comparative example provides a multi-effect composition, which differs from Example 1 only in that 0.3 parts of the supramolecular carnosine of Preparation Example 3 is replaced by 0.3 parts of carnosine. Other raw materials, amounts and preparation methods refer to Example 1.

[0084] Comparative Example 4

[0085] This comparative example provides a multi-effect composition, which differs from Example 1 only in that no recombinant collagen is added. Other raw materials, amounts and preparation methods refer to those of Example 1.

[0086] Comparative Example 5

[0087] This comparative example provides a multi-effect composition, which differs from Example 1 only in that cupuacu fruit nanocapsules are not added. Other raw materials, amounts and preparation methods refer to those of Example 1.

[0088] Comparative Example 6

[0089] This comparative example provides a multi-effect composition, which differs from Example 1 only in that supramolecular kapok is not added. Other raw materials, amounts and preparation methods refer to those of Example 1.

[0090] Comparative Example 7

[0091] This comparative example provides a multi-effect composition, which differs from Example 1 only in that supramolecular carnosine is not added. Other raw materials, amounts and preparation methods refer to those of Example 1.

[0092] Test Example 1

[0093] Antioxidant test

[0094] DPPH Radical Scavenging Ability: The ability to scavenge DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radicals reflects, to a certain extent, a substance's antioxidant capacity. A higher free radical scavenging rate indicates stronger antioxidant capacity and, therefore, stronger anti-aging properties. Therefore, the anti-aging effects of an anti-aging composition can be assessed by studying its DPPH free radical scavenging ability.

[0095] The compositions prepared in the examples and comparative examples were diluted with DMSO to a sample having a mass fraction of 2%, and the following test was performed:

[0096] Add 2.0 mL of 45.8 mg / L DPPH test solution and the sample to be tested to a test tube, add anhydrous ethanol to the total volume to 3 mL, shake well, react in the dark for 30 minutes, and measure the absorbance at a wavelength of 517 nm using a 1 cm cuvette, which is recorded as A10; add 2 mL of anhydrous ethanol and the corresponding volume of the sample to be tested to the test tube, add anhydrous ethanol to the total volume to 3 mL, and record the measured absorbance as A11; add 2 mL of DPPH test solution and 1 mL of anhydrous ethanol to the test tube, and record the measured absorbance as A12. Calculate the DPPH free radical scavenging rate (Y') of the test solution;

[0097] The calculation formula for DPPH free radical scavenging rate is:

[0098] Where A 10 A is the absorbance value of the system after the sample to be tested removes DPPH, 11 is the absorbance value of the system after removing DPPH from the sample blank control, A 12 is the absorbance value of the reaction system before adding the drug.

[0099] The test results are shown in Table 1.

[0100] Test Example 2

[0101] Anti-glycation test

[0102] The advanced glycation end products clearance test was performed, and the following reagents were prepared: 0.1 mol / L NaOH solution, 0.05 mol / L PBS phosphate buffer (pH 7.4), 10 mg / mL bovine serum albumin solution, 45 mg / mL glucose solution, 0.25 mmol / L aminoguanidine hydrochloride solution, and 200 mg / mL of the examples and comparative examples dissolved in water to obtain the sample solution to be tested.

[0103] Place PBS phosphate buffer, bovine serum albumin solution, and glucose solution in a constant temperature and humidity incubator, then add the sample solution to be tested and aminoguanidine hydrochloride solution, and incubate at 60°C for 40 hours. Take out each sample solution and detect it with a microplate reader. The fluorescence excitation / emission wavelength is 370 / 440nm, and the fluorescence absorption value is recorded. Make three parallel samples for each sample, and take the average value of the results. The inhibition rate of AEGs is calculated using the following formula:

[0104] Among them, A 样品 A represents the fluorescence intensity of the solution with the test substance and glucose added; 样品空白 A represents the fluorescence intensity of the solution with the test substance added but without glucose added; 阴性对照 A represents the fluorescence intensity of the glucose solution without the test substance; 缺糖阴性对照 It represents the fluorescence intensity of the solution containing neither test substance nor glucose.

[0105] The test results are shown in Table 1.

[0106] Test Example 3

[0107] Skin stratum corneum moisture content test

[0108] Testing instrument: Skin stratum corneum moisture content meter milipure;

[0109] Test sample: Antioxidant moisturizing lotion, comprising: 5% of the multi-effect composition prepared in the Examples and Comparative Examples, 4% squalane, 3% glycerin, 5% betaine, 2% hyaluronic acid, 2% sodium stearoyl lactylate, 0.5% guar gum, and the balance being purified water;

[0110] Testing phase: 2 weeks (D2), 4 weeks (D4);

[0111] Test subjects: 30 healthy women aged 20-45 years;

[0112] Measurement area: The test location is near the temple on the right side of the volunteer's face;

[0113] Test parameter explanation:

[0114] Water content of the stratum corneum: The higher the value, the more water the stratum corneum contains. Unit: Comeometer Unit, abbreviated as CU. Water growth rate (%) = (D n -D0)×100% / D0, where Dn is the water content of the subject's skin at D2 and D4, and D0 is the initial water content of the subject's skin. The test results are shown in Table 1.

[0115] Table 1

[0116] According to the data in the table, when recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers and supramolecular carnosine are used in a multi-effect composition in a specific proportion, the four raw materials work synergistically to jointly promote the antioxidant, anti-glycation and moisturizing effects, achieving the best effect.

[0117] It can be seen from Examples 1 and 4-5 that when the amount of cupuacu fruit nanocapsules, supramolecular kapok flowers or supramolecular carnosine in the composition is too much or too little, the antioxidant, anti-glycation and moisturizing effects will be affected; it can be seen from Examples 1 and 6 that acetone is used as a solvent when preparing cupuacu fruit nanocapsules, which has the best activity and high bioavailability. If ethanol is used instead, the skin care effect will be affected to a certain extent.

[0118] It can be seen from Example 1 and Comparative Examples 1-3 that when unprocessed cupuaçu oil, unprocessed kapok flower extract, and unprocessed carnosine are directly used as raw materials, the biological activity of the functional molecules in the multi-effect composition is reduced, and the antioxidant, anti-glycation, and moisturizing effects are greatly reduced.

[0119] It can be seen from Example 1 and Comparative Examples 4-7 that when the composition lacks any of recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers or supramolecular carnosine, the antioxidant, anti-glycation and moisturizing effects of the composition are greatly reduced.

[0120] The applicant declares that while the present application uses the above-mentioned embodiments to illustrate the process of the present application, the present application is not limited to the above-mentioned embodiments, which does not mean that the present application must rely on the above-mentioned embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present application, equivalent replacements for the raw materials of the present application's products, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present application.

[0121] The preferred embodiments of the present application are described in detail above. However, the present application is not limited to the specific details of the above embodiments. Within the technical concept of the present application, various simple modifications can be made to the technical solution of the present application, and these simple modifications all fall within the scope of protection of the present application.

[0122] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner unless there is any contradiction. In order to avoid unnecessary repetition, this application will not further describe various possible combinations.

Claims

1. A multi-effect composition containing recombinant collagen, comprising recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok and supramolecular carnosine.

2. The multi-effect composition according to claim 1, wherein: In parts by weight, the multi-effect composition comprises 0.1-0.5 parts of recombinant collagen, 1-10 parts of cupuacu fruit nanocapsules, 1-10 parts of supramolecular kapok flowers, and 0.1-1 parts of supramolecular carnosine; Optionally, the multi-effect composition comprises, by weight, 0.02-0.1 parts of recombinant collagen, 6-10 parts of cupuacu fruit nanocapsules, 6-10 parts of supramolecular kapok flowers, and 0.1-0.5 parts of supramolecular carnosine.

3. The multi-effect composition according to claim 1 or 2, wherein: The cupuacu fruit nanocapsules are prepared by the following method, which comprises: (1) dissolving cupuacu oil and pluronic in acetone to obtain a mixed solution; (2) dropping the mixed solution into distilled water that is being stirred, and continuing to stir, and removing acetone by natural evaporation to obtain nanoparticles; (3) adding chitosan to the continuously stirred nanoparticles to obtain the cupuacu fruit nanocapsules.

4. The multi-effect composition according to claim 3, wherein: The hydrophile-lipophile balance value of the Pluronic is 8-29; Optionally, the molecular weight of the chitosan is 3-20 kDa.

5. The multi-effect composition according to claim 3, wherein: The mass ratio of the cupuacu oil to pluronic is 1:(3-5); Optionally, the mass volume ratio of the cupuacu oil and acetone is (4-6) mg:1 mL; Optionally, the amount of distilled water used is 4 times that of acetone.

6. The multi-effect composition according to any one of claims 1 to 5, wherein: The supramolecular kapok flower is prepared by the following method, which comprises: extracting kapok flower powder by using supramolecular extracting solution to obtain the product.

7. The multi-effect composition according to claim 6, wherein: The solid-liquid ratio of the kapok flower powder to the supramolecular extract is 1:(30-70); Optionally, the supramolecular extract comprises betaine, glycerol and propylene glycol.

8. A method for preparing the multi-effect composition containing recombinant collagen according to any one of claims 1 to 7, comprising: The recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers and supramolecular carnosine are mixed evenly to obtain the product.

9. An antioxidant moisturizing lotion, the raw materials of which include: The multi-effect composition containing recombinant collagen according to any one of claims 1 to 7, an emollient, a humectant, an emulsifier and a thickener.

10. The antioxidant moisturizing emulsion according to claim 9, wherein: The mass percentage of the multi-effect composition in the antioxidant moisturizing emulsion is 1-10%.

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