Composition for tightening, relieving and repairing as well as preparation method and application of composition

Abstract

The invention discloses a composition for tightening, relieving and repairing as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw material formulas. The composition comprises the following components: soluble type 21 collagen, an euglena minuta extract, an acer rubrum extract and a white water lily flower extract. By optimizing the compounding proportion and extraction process of the plant extract, the efficient retention and synergistic effect of the active ingredients are ensured, so that the comprehensive performance and safety of the final product are improved, and the requirements of the market on efficient and mild skin care products are met.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care product raw material formula, and specifically relates to a firming, soothing and repairing composition and a preparation method and application thereof. Background Art

[0002] As consumers' demands for skin care products become increasingly diversified, the coordinated realization of multiple functions such as firming, soothing and repairing has become an important direction for the research and development of skin care products. However, most products in the existing technology tend to focus on a single function, such as only focusing on anti-wrinkle or moisturizing, which is difficult to meet users' needs for comprehensive skin care effects. In addition, commercially available products often rely on chemically synthesized ingredients (such as retinol, acid compounds, etc.). Although the short-term effects are significant, long-term use can easily cause skin irritation, sensitivity and other problems, and are especially not suitable for people with damaged barrier or sensitive skin.

[0003] In recent years, natural plant extracts have gradually attracted attention due to their mildness and rich active ingredients. However, the application of plant extracts in the existing technology still has many limitations: first, the synergistic effect between different plant components is not well studied, which makes it difficult to maximize the efficacy of the compound composition; second, traditional extraction processes, such as high temperature and long-term decoction, are easy to destroy heat-sensitive active ingredients and reduce the biological activity of the extracts; third, the molecular weight of the key ingredients in some formulas is too large, which makes it difficult to be absorbed through the skin, affecting their actual efficacy. Summary of the invention

[0004] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a skin care composition that can take into account the multiple functions of firming, anti-wrinkle, soothing and repairing, and has natural ingredients, scientific compatibility, and mild process. By optimizing the compounding ratio and extraction process of plant extracts, the efficient retention and synergistic effect of active ingredients are ensured, thereby improving the comprehensive performance and safety of the product and meeting the market demand for efficient and mild skin care products.

[0005] In order to achieve the above object, the present invention discloses the following technical solutions:

[0006] In a first aspect, the present invention provides a firming, soothing and repairing composition, which comprises the following components in parts by mass:

[0007]

[0008]

[0009] Preferably, the composition comprises the following components in parts by mass:

[0010]

[0011] Preferably, the preparation method of the white water lily extract comprises the following steps:

[0012] Step 1-1. Take dry Nymphaea alba flowers, crush and sieve them to obtain Nymphaea alba flower powder.

[0013] Step 1-2. Mix the Nymphaea alba flower powder with an ethanol aqueous solution and perform reflux extraction, then concentrate and dry the extract under reduced pressure to obtain the Nymphaea alba flower extract.

[0014] Further preferably, in step 1-1, the sieving is through a sieve with a mesh size of ≥30 meshes.

[0015] Further preferably, in step 1-2, the Nymphaea alba flower powder is mixed with an ethanol aqueous solution with a concentration of 70-75 v / v% according to a solid-liquid ratio of 1:(5-10) g / mL, reflux extracted at 60-70 °C for 3 times, 40 min each time, then the extracts are combined, concentrated under reduced pressure, and dried to a water content of ≤10% to obtain the Nymphaea alba flower extract.

[0016] In a second aspect, the present invention provides the use of the composition for firming, soothing and repairing described in the first aspect in the preparation of a skin care product having firming, anti-wrinkle, soothing and / or repairing effects.

[0017] In a third aspect, the present invention provides a firming, soothing and repairing essence, and the essence contains the composition for firming, soothing and repairing described in the first aspect.

[0018] Preferably, the addition amount of the composition for firming, soothing and repairing in the essence is 1-10 wt%.

[0019] Further preferably, the essence also contains PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerin, butylene glycol, 1,2-pentanediol, glycerol, methyl gluceth-20, hydroxyethyl cellulose, acryloyldimethyltaurine ammonium / VP copolymer, coco-caprylate / caprate, p-hydroxyacetophenone, and deionized water.

[0020] In a fourth aspect, the present invention provides a preparation method of the firming, soothing and repairing essence described in the third aspect, and the preparation method includes the following steps:

[0021] Step 2-1. Take 80% of the total amount of deionized water, add hydroxyethyl cellulose, and stir evenly to obtain solution A;

[0022] Step 2-2. Add the remaining deionized water to solution A, and sequentially add acryloyldimethyltaurine ammonium / VP copolymer, glycerol, butylene glycol, 1,2-pentanediol, methyl gluceth-20, and PEG / PPG / polybutylene glycol-8 / 5 / 3, heat to 75-80 °C and stir until completely dissolved to obtain solution B;

[0023] Step 2-3. Cool the B solution to 50°C, and slowly add cetearyl octanoate / caprate to it to obtain the C solution by dispersion;

[0024] Step 2-4. Cool the C solution to 40°C, add p-hydroxyacetophenone and the composition for firming, soothing and repairing, stir and filter to remove impurities to obtain the firming, soothing and repairing essence.

[0025] Preferably, in Step 2-1, hydroxyethyl cellulose is slowly sprinkled at normal temperature and 200 r / min, stirred evenly and then left to swell for 30 min to obtain the A solution;

[0026] In Step 2-2, ammonium acryloyldimethyltaurate / VP copolymer, glycerol, butanediol, 1,2-pentanediol, methyl gluceth-20 and PEG / PPG / polybutylene glycol-8 / 5 / 3 are added in sequence, heated to 75°C, and stirred at 300 r / min for 20 min until completely dissolved to obtain the B solution;

[0027] In Step 2-3, cetearyl octanoate / caprate is slowly added to it, and dispersed at 3000 r / min for 20 min to obtain the C solution;

[0028] In Step 2-4, p-hydroxyacetophenone and the composition for firming, soothing and repairing are added, stirred at 200 r / min for 10 min, and filtered through a 200-mesh sieve to remove impurities to obtain the firming, soothing and repairing essence.

[0029] In the present invention:

[0030] Soluble type 21 collagen has many effects in skin care. It can adsorb and lock moisture to form a moisturizing film, making the skin moist and smooth; it can combine with collagen fibers to promote their assembly and repair, enhance skin elasticity and firmness, and reduce wrinkles and sagging; it has antioxidant properties, resists free radicals, reduces age spots and wrinkles, and delays aging; it can promote skin cell regeneration and repair, accelerate the healing of damaged skin, such as sunburn and dullness, and also promote metabolism; it can inhibit the growth and reproduction of bacteria on the skin surface, reduce inflammation, relieve redness, swelling and itching, and soothe sensitive skin; it can inhibit the formation of melanin, accelerate keratin exfoliation, and whiten the skin.

[0031] The extract of Euglena gracilis is rich in natural polypeptide molecules and has significant antioxidant effects. It can effectively scavenge free radicals, reduce skin damage caused by free radicals, and at the same time promote the repair and regeneration of skin cells. In addition, it also has moisturizing and nourishing effects, helps maintain the water balance of the skin, makes the skin softer and more elastic. The extract of Euglena gracilis also has certain anti-inflammatory properties, which can help reduce skin redness and discomfort, thereby enhancing the skin barrier function and improving the overall skin health condition.

[0032] The extract of Acer rubrum has antioxidant, anti-inflammatory and soothing effects, which helps to protect the skin from free radical damage, reduce skin inflammation and enhance the skin barrier function.

[0033] The extract of Nymphaea alba contains rich components such as polyphenols, flavonoids, terpenoids, alkaloids, vitamins, minerals, amino acids and organic acids. Polyphenols, terpenoids and flavonoids have antioxidant, anti-inflammatory and free radical scavenging abilities. Vitamins and minerals participate in the skin's metabolic process, and amino acids and organic acids provide nutrition for the skin and improve skin quality.

[0034] Advantages of the present invention:

[0035] When the soluble type 21 collagen, Euglena gracilis extract, Acer rubrum extract and Nymphaea alba extract in the present invention are compounded and used within a specific ratio range, they exhibit excellent efficacy in firming, soothing and repairing. Detailed implementation manners

[0036] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0037] Unless otherwise specified, the test methods used in the examples are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels; the percentages mentioned in the examples and comparative examples, unless otherwise specified, are all mass percentages.

[0038] In the present invention:

[0039] Soluble type 21 collagen: purchased from Ideal Medical Technology (Shanghai) Co., Ltd.;

[0040] Euglena gracilis extract: purchased from Hemei Biotechnology (Zhejiang) Co., Ltd.;

[0041] Acer rubrum extract: purchased from Guangzhou Yihe Trading Co., Ltd.;

[0042] Nymphaea alba: Latin name Nymphaea alba L., purchased from SEPPIC S.A., France.

[0043] I. Example

[0044] Preparation of Nymphaea alba extract

[0045] Step 1-1. Take fresh Nymphaea alba and dry it to constant weight, place it in a high-speed grinder for grinding, and after grinding, pass through a sieve with a mesh size of ≥30 to obtain Nymphaea alba powder;

[0046] Step 1-2. Mix the white water lily pollen with an ethanol aqueous solution at a concentration of 75 v / v% according to a material-liquid ratio of 1:10 g / mL, reflux and extract 3 times at 70 °C for 40 min each time. Then combine the extracts, concentrate the extracts under reduced pressure, and dry to a water content of ≤10% to obtain the white water lily extract.

[0047] Tightening, soothing and repairing composition

[0048] Accurately weigh the raw materials according to the raw material mass ratio in Table 1, and then compound the raw materials to obtain the tightening, soothing and repairing composition.

[0049] Table 1 Raw material mass ratio of the tightening, soothing and repairing composition

[0050] Raw materials Composition 1 Composition 2 Composition 3 Composition 4 Soluble type XXI collagen 0.3 0.45 0.6 - Extract of Euglena gracilis 9 11 13 11 Extract of Acer rubrum 1 1.5 2 1.5 Extract of Nymphaea alba 0.1 0.2 0.3 0.2 Raw materials Composition 5 Composition 6 Composition 7 Composition 8 Soluble type XXI collagen 0.45 0.45 0.45 1 Extract of Euglena gracilis - 11 11 1 Extract of Acer rubrum 1.5 - 1.5 1 Extract of Nymphaea alba 0.2 0.2 - 1

[0051] Efficacy verification

[0052] Add the above composition to the solvent according to the mass percentage of the raw materials in Table 2, and stir at 300 r / min and 30 - 40 °C for 2 h to obtain a solution containing the tightening, soothing and repairing composition as the test solution for efficacy verification.

[0053] Table 2 Raw material mass percentage

[0054]

[0055] 1 Tightening and anti-wrinkle

[0056] 1.1 Solution preparation

[0057] (1) Test samples: Respectively take the test solutions 1 - 8 obtained in Table 2, and dilute them 100 times with Tris-HCl buffer to a concentration of 1 wt%;

[0058] (2) Prepare Tris-HCl buffer (0.1 M, pH = 8.0): Weigh 2.42 g of Tris into a beaker, add 200 mL of ultrapure water, and adjust the pH to 8.0 with concentrated HCl after complete dissolution.

[0059] (3) Prepare substrate solution AAAPVN (2 mM): Weigh 4.51 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it with 5 ml of Tris-HCl buffer.

[0060] (4) Prepare porcine pancreatic elastase solution (0.171 U / mL): Take 280 μL and dissolve it in 10 mL of Tris-HCl buffer.

[0061] 1.2 Sample addition

[0062] The experiment was divided into a test sample group, a model control group, and a blank control group. Each group had 4 replicate wells, and the addition amounts of the solutions in each group were shown in Table 3.

[0063] Table 3 Experimental grouping and sample addition

[0064]

[0065] 1.3 Determination

[0066] Leave it at room temperature for 15 min for reaction, and measure its absorbance at 420 nm with an enzyme-labeled instrument.

[0067] 1.4 Result calculation

[0068]

[0069] In the formula: A0 - the average absorbance of the blank control well;

[0070] A1 - the average absorbance of the sample well;

[0071] A2 - the average absorbance of the model control well.

[0072] 1.5 Data statistics

[0073] Use the SPSS 19.0 statistical software package for statistical analysis. If the test data is normally distributed, the independent T-test method is used for statistical analysis; if the test data is non-normally distributed, the rank sum test method is used for statistical analysis. The statistical method uses a two-tailed test, and the test level a = 0.05.

[0074] 1.6 Experimental results

[0075] Table 4 Detection results of the inhibitory rate of porcine pancreatic elastase

[0076]

[0077]

[0078] Note: "a" indicates a significant difference compared with the model control, "aa": p < 0.01, "aaa": p < 0.001; "b" indicates a significant difference compared with test sample 2, "bb": p < 0.01, "bbb": p < 0.001.

[0079] 1.7 Result analysis

[0080] According to the data in Table 4, there were significant differences in the inhibitory rates of test samples 1 - 8 on porcine pancreatic elastase. The inhibitory rate of test sample 2 was the highest, reaching 47.12%, and there was a highly significant difference compared with the model control group (p < 0.001), indicating that its firming and anti-wrinkle effect was the best.

[0081] The inhibition rates of test samples 1 and 3 were 42.43% and 45.84% respectively, showing no significant difference from test sample 2, indicating that soluble type XXI collagen, Euglena gracilis extract, Acer rubrum extract and Nymphaea alba flower extract in the present invention exhibit good efficacy within a certain ratio range.

[0082] The inhibition rates of test samples 4 - 8 decreased significantly, indicating that the absence or excess of any one component in soluble type XXI collagen, Euglena gracilis extract, Acer rubrum extract and Nymphaea alba flower extract has a great impact on the performance of the formulation. Among them, although the inhibition rate of test sample 8 is higher than that of compositions 4 - 7, it is still significantly lower than that of compositions 1 - 3, suggesting that the balance of each component in the formulation is crucial, and the excessive presence of a single component may destroy the synergistic effect of the overall formulation.

[0083] 2 Soothing effect

[0084] 2.1 Main reagents

[0085] Mouse mononuclear macrophages (RAW264.7): Purchased from Guangzhou Saiku Biotechnology; DMEM high - glucose medium: Purchased from Enzyme - linked Biotechnology; Trypsin - EDTA (containing phenol red): Purchased from Enzyme - linked Biotechnology; Fetal bovine serum: Purchased from Enzyme - linked Biotechnology; IL - 1β ELISA kit: Purchased from Enzyme - linked Biotechnology; LPS: Lipopolysaccharide, purchased from Enzyme - linked Biotechnology.

[0086] 2.2 Test samples

[0087] Test sample group: The safe working concentration was determined by cytotoxicity test (MTT method) for test solutions 1 - 8. The test verified that when test solutions 1 - 8 were diluted to a concentration ≤ 2 wt%, the cell viability was ≥ 90%. Finally, a concentration of 1 wt% was determined as the working concentration for the following experiments.

[0088] 2.2 Experimental methods

[0089] 2.2.1 Cell activation

[0090] After resuscitating the cryopreserved mouse mononuclear macrophages (RAW264.7), they were cultured in a T25 culture flask with 5 mL of complete medium (DMEM high - glucose medium containing 10% fetal bovine serum), placed in an incubator at 37°C and 5 v / v% CO2, and the medium was changed after 24 h of culture for sub - culture and standby.

[0091] 2.2.2 Grouping and administration

[0092] The RAW 264.7 cells cultured to the logarithmic growth phase were seeded at 1×10 5Inoculate at a density of cells per well in a 96-well plate and divide into groups: normal group, model control group, and test sample group. Repeat 6 wells in each group as duplicates. Except for the cells in the normal group, add complete medium containing LPS at a final concentration of 1 μg / mL to the cells in the other groups. After culturing for 24 h, add samples according to the grouping shown in Table 5 and culture for another 24 h:

[0093] Table 5 Stimulation and drug administration

[0094]

[0095] After culturing for 24 h, collect the cell supernatant and determine the content of IL-1β in the cell supernatant according to the operating steps of the ELISA kit.

[0096] 2.3 Data statistics

[0097] Use the SPSS 19.0 statistical software package for statistical analysis. If the test data is normally distributed, use the independent T-test method for statistical analysis; if the test data is not normally distributed, use the rank sum test method for statistical analysis. All statistical methods use two-tailed tests, and the significance level α = 0.05.

[0098] 2.4 Experimental results

[0099]

[0100] Where: A0 - the average value of the IL-1β content in the normal group;

[0101] A1 - the average value of the corresponding IL-1β content in each test sample group;

[0102] A2 - the average value of the IL-1β content in the model control group.

[0103] Table 6 IL-1β content and inhibition rate in each group

[0104]

[0105] 2.5 Result analysis

[0106] According to the results in Table 6, it can be seen that the inhibition rates of test sample groups 1, 2, and 3 on inflammatory factors reached 83.54%, 84.54%, and 79.68% respectively. Among them, test sample group 2 had the best effect and was significantly different from the model control group (p < 0.001). This indicates that soluble type XXI collagen, Euglena gracilis extract, Acer rubrum extract, and Nymphaea alba flower extract in the present invention also show good anti-inflammatory and soothing effects within this ratio range.

[0107] 3 Repair efficacy

[0108] 3.1 Principle

[0109] Keratinocytes are important cells in the epidermal layer. The behavior of keratinocytes has an important impact on whether the skin can recover in time after being damaged. By comparing the migration changes of keratinocytes in the blank control group and the sample group, it can be evaluated whether the test sample is beneficial to the reconstruction of the barrier function in the case of damaged skin barrier.

[0110] 3.2 Main reagents

[0111] HaCaT human keratinocytes: purchased from Guangzhou Saiku Biotech; fetal bovine serum: purchased from Enzyme-linked Biotech; DMEM high-glucose medium: purchased from Enzyme-linked Biotech; penicillin-streptomycin solution: purchased from Enzyme-linked Biotech; trypsin-EDTA solution: purchased from Enzyme-linked Biotech.

[0112] 3.3 Experimental grouping

[0113] The test sample group is the sample solution prepared above by diluting the test solution 2 with cell culture medium to the corresponding test concentration, specifically as follows:

[0114] Test sample group 1: complete medium containing 1 wt% of test solution 2;

[0115] Test sample group 2: complete medium containing 0.5 wt% of test solution 2;

[0116] Test sample group 3: complete medium containing 0.1 wt% of test solution 2.

[0117] Blank control group: complete medium.

[0118] 3.4 Cell preparation

[0119] The cryopreserved cell culture was subcultured stably twice and then inoculated into a 24-well plate, and the plate was placed in a 5 v / v% CO2 incubator and cultured overnight.

[0120] 3.5 Testing method

[0121] Remove the cell culture insert from the monolayer cells in culture, and continue to culture the cells with the medium containing the sample. Observe the area of the cells migrating to the scratched area after 24 h, and calculate the change in the area of the cells migrating to the scratched area.

[0122] 3.6 Evaluation criteria

[0123] Compared with the blank control, the scratch between cells in the test sample group decreased and there was a significant difference (P < 0.05), indicating that the test sample group has the ability to promote keratinocyte migration in this mode.

[0124] 3.7 Experimental results

[0125] Under normal culture conditions, the migration of keratinocytes is shown in Table 7.

[0126] Table 7 Keratinocyte Migration Rate

[0127]

[0128] Note: "*" indicates significant difference compared with the blank control group. "*": P < 0.05, "**": P < 0.01.

[0129] Compared with the blank control group, the migration area of keratinocytes in the complete medium containing 1 wt% of the test solution 2 increased significantly, and the migration rate was 23.557%; the migration area of keratinocytes in the complete medium containing 0.5 wt% of the test solution 2 increased significantly, and the migration rate was 13.629%; the migration rate of keratinocytes in the complete medium containing 0.1 wt% of the test solution 2 was 5.107%. As the concentration of the test solution 2 increased, the keratinocyte migration rate showed a continuous increase, indicating that the composition provided by the present invention can effectively promote skin repair and has the effect of repairing the skin barrier.

[0130] II. Application Example

[0131] Preparation of Firming, Soothing and Repairing Essence

[0132] Accurately weigh the raw materials according to the mass percentages in Table 8;

[0133] Step 2-1. Take deionized water accounting for 80% of the total water volume, start the stirring paddle, and slowly sprinkle hydroxyethyl cellulose at normal temperature and 200 r / min. After stirring evenly, let it swell for 30 min to obtain Solution A;

[0134] Step 2-2. Transfer Solution A to the main reaction kettle, add the remaining deionized water, and successively add acryloyldimethyltaurate / VP copolymer, glycerol, butanediol, 1,2-pentanediol, methyl gluceth-20 and PEG / PPG / polybutylene glycol-8 / 5 / 3. Heat up to 75 °C and stir at 300 r / min for 20 min until completely dissolved to obtain Solution B;

[0135] Step 2-3. Cool Solution B to 50 °C, and slowly add cetyl octanoate / caprate to it. Disperse at 3000 r / min for 20 min to obtain Solution C;

[0136] Step 2-4. Cool Solution C to 40 °C, add p-hydroxyacetophenone and the firming, soothing and repairing composition 2 prepared in the example, stir at 200 r / min for 10 min, and filter through a 200-mesh sieve to remove impurities. Fill into a light-proof and sealed container to obtain the firming, soothing and repairing essence.

[0137] Table 8 Mass Percentages of Raw Materials for the Essence

[0138]

[0139] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements on some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composition for firming, soothing and repairing, characterized in that, By mass parts, the composition contains the following components: Soluble type 21 collagen 0.1 - 3 parts; Euglena gracilis extract 0.1 - 15 parts; Acer rubrum extract 0.1 - 3 parts; Nymphaea alba flower extract 0.1 - 2 parts.

2. The composition for firming, soothing and repairing according to claim 1, characterized in that, By mass parts, the composition contains the following components: Soluble type 21 collagen 0.3 - 0.6 parts; Euglena gracilis extract 9 - 13 parts; Acer rubrum extract 1 - 2 parts; Nymphaea alba flower extract 0.1 - 0.3 parts.

3. The composition for firming, soothing and repairing according to claim 1 or 2, characterized in that, The preparation method of the Nymphaea alba flower extract comprises the following steps: Step 1 - 1. Take dry Nymphaea alba flowers, crush and sieve them to obtain Nymphaea alba flower powder; Step 1 - 2. Mix the Nymphaea alba flower powder with an ethanol aqueous solution and perform reflux extraction, then concentrate and dry the extract under reduced pressure to obtain the Nymphaea alba flower extract.

4. The composition for firming, soothing and repairing according to claim 3, characterized in that, In step 1 - 1, the sieving is through a sieve of ≥30 mesh.

5. The composition for firming, soothing and repairing according to claim 3, characterized in that, In step 1 - 2, the Nymphaea alba flower powder is mixed with an ethanol aqueous solution with a concentration of 70 - 75 v / v% according to a solid - liquid ratio of 1:(5 - 10) g / mL, reflux - extracted at 60 - 70 °C for 3 times, 40 min each time, then the extracts are combined, concentrated under reduced pressure, and dried to a water content of ≤10% to obtain the Nymphaea alba flower extract.

6. Use of the firming, soothing and repairing composition according to any one of claims 1 - 5 in the preparation of a skin - care product having firming, anti - wrinkle, soothing and / or repairing effects.

7. A firming, soothing and repairing essence, characterized in that, The essence contains the firming, soothing and repairing composition according to any one of claims 1 - 5; The addition amount of the firming, soothing and repairing composition in the essence is 1 - 10 wt%.

8. The firming, soothing and repairing essence according to claim 7, wherein The essence also contains PEG / PPG / polybutylene glycol - 8 / 5 / 3 glycerin, butylene glycol, 1,2 - pentanediol, glycerol, methyl gluceth - 20, hydroxyethyl cellulose, acryloyldimethyltaurine ammonium / VP copolymer, coco - caprylate / caprate, p - hydroxyacetophenone, deionized water.

9. The preparation method of the firming, soothing and repairing essence as claimed in claim 8, characterized in that, The preparation method comprises the following steps: Step 2 - 1. Take 80% of the total amount of deionized water, add hydroxyethyl cellulose, and stir evenly to obtain solution A; Step 2 - 2. Add the remaining deionized water to solution A, and sequentially add acryloyldimethyltaurine ammonium / VP copolymer, glycerol, butylene glycol, 1,2 - pentanediol, methyl gluceth - 20 and PEG / PPG / polybutylene glycol - 8 / 5 / 3, heat to 75 - 80 °C and stir until completely dissolved to obtain solution B; Step 2 - 3. Cool solution B to 50 °C, and slowly add coco - caprylate / caprate to it to disperse and obtain solution C; Step 2 - 4. Cool solution C to 40 °C, add p - hydroxyacetophenone and the firming, soothing and repairing composition, stir and filter to remove impurities to obtain the firming, soothing and repairing essence.

10. The preparation method according to claim 9, characterized in that, In step 2 - 1, hydroxyethyl cellulose is slowly added at room temperature and 200 r / min, stirred evenly and left to swell for 30 min to obtain solution A; In step 2-2, ammonium acryloyldimethyltaurate / VP copolymer, glycerin, butylene glycol, 1,2-pentanediol, methyl gluceth-20, and PEG / PPG / polybutylene glycol-8 / 5 / 3 are added in sequence, the temperature is raised to 75 °C, and stirring is carried out at 300 r / min for 20 min until completely dissolved to obtain solution B; In step 2-3, coco-caprylate / caprate is slowly added thereto, and dispersion is carried out at 3000 r / min for 20 min to obtain solution C; In step 2-4, hydroxyacetophenone and a composition for firming, soothing and repairing are added, stirring is carried out at 200 r / min for 10 min, and filtration is carried out through a 200-mesh sieve to remove impurities to obtain a firming, soothing and repairing essence.

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