Bottle-type culture medium apparatus

Abstract

Provided is a bottle-type culture medium apparatus, comprising a bottle-type base body, an inner support and a bottle cap. The inner support is mounted at a bottle mouth of the bottle-type base body, and divides the internal space of the bottle-type base body into a solid storage area located inside the inner support and a liquid storage area located outside the inner support; the bottle cap is capped onto the bottle mouth of the bottle-type base body; when in a use state, the solid storage area is in communication with the liquid storage area. The provided bottle-type culture medium apparatus can not only achieve solid-liquid separation, but can also achieve light-proof storage at room temperature by means of sealing, can be quickly assembled and disassembled, is convenient and quick to use, and does not easily produce sediment.

Description

Bottle-type culture medium device Technical Field

[0001] The invention belongs to the technical field of bottle-type microbiology devices, and in particular relates to a bottle-type culture medium device. Background Art

[0002] Culture media refers to a nutrient matrix composed of a combination of various nutrients that supports the growth and reproduction of microorganisms, plants, or animals (or tissues). It generally contains several major categories of substances, including carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, and water. Based on their composition and purpose, culture media can be divided into basal, enrichment, selective, differential, and anaerobic media. Culture media are primarily used for the growth and reproduction of living organisms, such as microorganisms, plant, or animal cells; for the identification, classification, and enumeration of microorganisms; for the storage and maintenance of bacterial strains; and for scientific research, such as exploring the physiological and biochemical mechanisms of life. Liquid culture media are liquid media, consisting of solutions of water and various nutrients. To prepare a liquid culture medium, the various components must be dissolved in a liquid such as water. Compared to solid culture media, liquid culture media are more convenient to use, offer larger culture capacities, and allow for better control of culture conditions. However, existing liquid culture media have strict storage requirements, such as temperature and humidity. They require cold storage for transportation, have a short shelf life, are inconvenient to store, and can also produce sediment that affects subsequent use by personnel.

[0003] Summary of the Invention

[0004] In response to the deficiencies of the prior art, the present invention provides a bottle-type culture medium device that can not only separate solids and liquids, but also achieve light-proof and room-temperature storage through multi-stage sealing. It can be quickly disassembled and assembled, is convenient and quick to use, and is not prone to producing sediment.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] A bottle-shaped culture medium device comprises a bottle-shaped base, an inner tray, and a bottle cap. The inner tray is mounted on the bottle mouth of the bottle-shaped base and divides the internal space of the bottle-shaped base into a solid storage area located within the inner tray and a liquid storage area located outside the inner tray. The bottle cap is attached to the bottle mouth of the bottle-shaped base. When in use, the solid storage area is connected to the liquid storage area. Compared with the existing technology, the present invention has the following advantages: by storing liquid culture medium in the bottle-shaped base and solid culture medium in the inner tray, the present invention can achieve separation of solid and liquid, and realize light-proof and room temperature storage.

[0007] In one embodiment, the inner support includes a storage cylinder and a blocking piece; the storage cylinder is detachably mounted on the bottle mouth of the bottle-shaped base and extends toward the interior of the bottle-shaped base; the blocking piece covers the bottle mouth of the storage cylinder at one end away from the bottle mouth of the bottle-shaped base.

[0008] In another embodiment, a downwardly extending rod is protruding from the inner top wall of the bottle cap, and the lower end of the rod extends out of the bottle cap; when in use, the bottle cap moves downward, and the rod can push open the baffle, connecting the solid storage area with the liquid storage area.

[0009] In another embodiment, a positioning cylinder surrounding the outer periphery of the rod body is protruded from the inner top wall of the bottle cap, and the positioning cylinder can be inserted into the storage cylinder body and have an interference fit therewith.

[0010] In another embodiment, a detachable pull ring is further included, wherein the detachable pull ring is located between the bottle base and the bottle cap to limit the downward movement of the bottle cap.

[0011] In another embodiment, the inner support further includes a filter membrane cartridge, which is sleeved on the bottom of the storage cylinder and covered with a filter membrane on a side away from the storage cylinder, and the filter membrane is located below the baffle.

[0012] In another embodiment, the inner diameter of the storage cylinder gradually decreases from top to bottom, and the outer diameter of the positioning cylinder is smaller than the maximum inner diameter of the storage cylinder and larger than the minimum inner diameter of the storage cylinder.

[0013] In another embodiment, the baffle is provided with an upwardly extending rod; in use, the bottle cap moves downward, the rod is pressed downward and can push open the baffle, connecting the solid storage area with the liquid storage area.

[0014] In another embodiment, a pressing block facing the rod body is protruded from the inner top wall of the bottle cap, and the pressing block can press down the rod body.

[0015] In another embodiment, a positioning cylinder is protruding from the inner top wall of the bottle cap, and the positioning cylinder can be inserted into the storage cylinder and have an interference fit therewith.

[0016] In another embodiment, a sealing film is provided on the opening cover of the storage cylinder facing the bottle cap.

[0017] In another embodiment, a positioning cylinder is protruding from the inner top wall of the bottle cap, and the positioning cylinder is located above the sealing film and separated by a distance; in use, the bottle cap moves downward, and the positioning cylinder presses down the sealing film.

[0018] In another embodiment, the top of the inner support is provided with a flange for engaging with the bottle-shaped base.

[0019] In another embodiment, a base is provided at the bottom of the bottle-shaped base, a clamping cylinder is provided at the upper end of the base, a placement plate that slides in the up and down directions is provided inside the clamping cylinder, and a fixing component for fixing the bottle-shaped base is provided inside the clamping cylinder.

[0020] In another embodiment, the fixing assembly includes two slot frames arranged on the inner arc wall of the clamping cylinder, the two slot frames correspond to each other on the left and right, and the slot frames are located above the placement tray; two clamping blocks are provided at the lower end of the outer arc surface of the bottle-shaped base, and the clamping blocks are respectively inserted into the corresponding slot frames, and a mounting spring is provided in the clamping cylinder, whose two ends are respectively connected to the placement tray and the base.

[0021] In another embodiment, a limit assembly is further included, the limit assembly comprising a cavity ring, a connecting rod, a plug post, a locking frame and an adjustment seat arranged at the upper end of the bottle-shaped base; two locking frames are slidably connected in the cavity ring through two plug posts, the two plug posts are respectively located on the radial front and rear sides of the bottle-shaped base, one end of the plug post passes through the outside of the cavity ring and is connected to a stop head for engaging with the cavity ring; a socket for engaging with the plug post (208) is provided on the outer arc surface of the bottle cap at a position corresponding to each plug post, and a reset spring is connected between the cavity ring and each locking frame. The reset spring is sleeved outside the corresponding plug column; the two ends of each locking frame are rotatably connected to a connecting rod, and the two connecting rods located on the same locking frame are symmetrical about the reset spring connected to the locking frame; two adjustment seats are slidably connected to the cavity ring through two sliding columns, and the two adjustment seats are respectively located on the radial left and right sides of the bottle-shaped base, and one end of the sliding column extends out of the cavity ring and is connected to a pressing plate (202); the ends of the two connecting rods rotatably connected on the same locking frame, which are away from the locking frame, are rotatably connected to the corresponding ends of the two adjustment seats.

[0022] In another embodiment, a plurality of anti-slip protrusions are provided on the outer peripheral surface of the bottle cap.

[0023] In another embodiment, a sealing assembly is further included, which includes at least one of a rectangular sealing ring, an arc-shaped sealing ring and a trapezoidal sealing groove protruding from the outer peripheral wall of the bottleneck of the bottle-shaped base or the inner peripheral wall of the bottle cap, and the inner peripheral wall of the bottle cap or the outer peripheral wall of the bottleneck of the bottle-shaped base is respectively provided with a corresponding rectangular sealing groove, arc-shaped sealing groove and trapezoidal sealing groove.

[0024] In another embodiment, the sealing assembly is a sealing ring disposed between the bottle cap and the limiting assembly.

[0025] In another embodiment, mounting holes are respectively provided at the four corners of the base, or double-sided tape is adhered to the lower end of the base.

[0026] In another embodiment, a label is adhered to the outer peripheral surface of the bottle-shaped base.

[0027] As a further improvement of the present invention, a label is attached to the outer arc surface of the bottle-shaped base. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] FIG1 is a schematic diagram of the overall structure of a bottle-type culture medium device according to Example 1 of the present invention;

[0030] FIG2 is a cross-sectional view of FIG1 ;

[0031] FIG3 is a schematic diagram of the three-dimensional structure of the inner support in FIG2 ;

[0032] FIG4 is a top view of the inner support in FIG2 ;

[0033] FIG5 is a partial structural cross-sectional view of Example 2 of the bottle-type culture medium device of the present invention;

[0034] FIG6 is a partial cross-sectional view of Example 2 of the bottle-type culture medium device of the present invention after the filter membrane cartridge is installed;

[0035] FIG7 is a cross-sectional view of the inner support of the bottle-type culture medium device in Example 2 of the present invention;

[0036] FIG8 is a schematic structural diagram of Example 3 of the bottle-type culture medium device of the present invention;

[0037] FIG9 is a schematic diagram of the explosion structure of FIG8 ;

[0038] FIG10 is a schematic structural diagram of a fixing assembly in Example 3 of a bottle-type culture medium device of the present invention;

[0039] FIG11 is a cross-sectional view of FIG10;

[0040] FIG12 is a schematic structural diagram of a bottle-shaped base in Example 3 of a bottle-shaped culture medium device of the present invention;

[0041] FIG13 is an enlarged schematic diagram of point A in FIG12;

[0042] FIG14 is a cross-sectional view of a bottle-shaped base body in Example 3 of the inventive bottle-shaped culture medium device;

[0043] FIG15 is an enlarged schematic diagram of point B in FIG14;

[0044] Figure 16 is a schematic structural diagram of the bottle cap and inner support in Example 3 of the invented bottle-type culture medium device.

[0045] In the figure: 101, base; 102, mounting hole; 103, bottle-shaped base; 104, scale mark; 105, label; 106, clamping cylinder; 107, inner support; 1071, sealing membrane; 108, filter membrane cylinder; 1081, filter membrane; 109, clamping block; 110, slot frame; 111, mounting spring; 112, placement plate; 113, double-sided tape; 114, baffle; 1141, slit; 115, flange; 116, positioning cylinder; 117, storage cylinder; 118, detachable pull ring; 1181, buckle; 119, extension cylinder; 201, cavity ring; 2011, ring body; 2012, support plate ;202, pressing plate;203, bottle cap;2031, pressing block;204, anti-slip protrusion 1;205, rod body;206, socket;207, connecting rod;208, plug column;209, return spring;210, locking frame;211, anti-slip protrusion 2;212, adjustment seat;213, sliding column;30, sealing assembly;301, sticking ring;302, rectangular sealing groove;303, arc-shaped sealing groove;304, trapezoidal sealing groove;305, rectangular sealing ring;306, arc-shaped sealing ring;307, trapezoidal sealing ring;308, docking groove ring;309, sealing ring;40, fixing assembly;50, limiting assembly. DETAILED DESCRIPTION

[0046] In order to better understand the present invention, the content of the present invention is further clearly set forth below in conjunction with the examples, but the protection content of the present invention is not limited to the following examples. In the following description, a large number of specific details are provided in order to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details.

[0047] To address the difficulty of storing and transporting liquid culture media in existing technologies, the inventors of this application have developed a bottle-type culture medium device that prepares the components of the nutrient matrix as a solid powder and stores it in a sealed container. This powder is then mixed with a liquid only when needed for cultivation, reducing storage and transportation requirements and enabling the culture medium to be stored at room temperature. Furthermore, the inventors designed the bottle-type culture medium device of this invention to address the different states of liquid culture media during transportation and storage, as well as during cultivation.

[0048] Among them, when in the transportation and storage state, the bottle-type culture medium device of the present invention divides the internal space of the bottle-type base of the bottle-type culture medium device into a solid storage area and a liquid storage area that are isolated from each other through an inner support installed at the bottle mouth, and then the bottle cap is closed to the bottle mouth of the bottle-type base to seal the bottle-type culture medium device for convenient transportation, moisture-proofing, etc. When in use, the solid storage area and the liquid storage area are connected through the rod body of the bottle-type culture medium device, so that the solid in the solid storage area is mixed with the liquid in the liquid storage area to carry out culture. In one embodiment, the bottle-type culture medium device of the present invention is used as a complete culture medium, and various necessary nutrient dry powder components are stored in the solid storage area. When in use, liquid is added to the liquid storage area, which improves the convenience of operation for the experimenter, and the dry powder is easy to store, and the nutrient dry powder can be preserved even at room temperature.

[0049] Specifically, the specific embodiment 1 of the bottle-type culture medium device of the present invention is as follows:

[0050] As shown in FIG1 , FIG2 and FIG3 , the bottle-type culture medium device includes a bottle-type base 103 , an inner support 107 and a bottle cap 203 . In this embodiment, the bottle-type base 103 , the inner support 107 and the bottle cap 203 are all made of plastic.

[0051] The bottle base 103 is used to store liquid culture media, and the inner support 107 is used to store solid culture media. As shown in Figure 2, the bottle base 103 is a hollow bottle with a mouth at the top. The bottle cap 203 fits over the mouth of the bottle base 103 and is threadedly connected to the neck of the bottle base 103. It is understood that the bottle base 103 can be, but is not limited to, bottles with circular or rectangular cross-sections as known in the art. A positioning tube 116 and a rod 205 are protruding from the inner top wall of the bottle cap 203. In this embodiment, the rod 205 is protruding near the middle of the inner top wall of the bottle cap 203 and extends downward. The positioning tube 116 surrounds the outer periphery of the rod 205 and is coaxial with the rod 205. The lower end of the rod 205 extends outside the bottle cap 203 and can extend downward into the interior of the bottle base 103 during use. A plurality of anti-slip protrusions 204 are arranged side by side along the circumferential direction on the outer peripheral surface of the bottle cap 203 , and the plurality of anti-slip protrusions 204 extend in the up-down direction.

[0052] As shown in Figures 3 and 4, the inner support 107 includes a flange 115, a storage cylinder 117, and a baffle 114. The storage cylinder 117 extends in the vertical direction. The flange 115 surrounds the outside of the opening of the storage cylinder 117 on the side close to the bottle cap 203, and the outer diameter of the flange 115 is larger than the diameter of the bottle base 103. The storage cylinder 117 is used to be inserted into the bottle base 103. The flange 115 engages with the bottle base 103 to allow the inner support 107 to be detachably suspended in the bottle base 103. The baffle 114 is provided on the opening of the storage cylinder 117 away from the bottle cap 203. When not in use, the rod 205 extends above the baffle 114 and is separated from it by a distance. In this embodiment, the baffle 114 is a thin film made of tin foil or the like that covers the storage cylinder 117 and is detachably mounted on the storage cylinder 117. In another embodiment, the baffle 114 is integrally formed with the storage cylinder 117 and is provided with a slit 1141 projected along the axis of the storage cylinder 117. The slit 1141 is annular and slightly smaller in height than the baffle 114, allowing the baffle 114 to maintain a seal against the storage cylinder 117 while being able to separate from the storage cylinder 117 when subjected to external force. The baffle 114 is used to prevent solid culture medium from entering the bottle-shaped base 103. Furthermore, the inner support 107 further includes an extension tube 119 and a filter membrane cartridge 108. The extension tube 119 is coaxially connected to the bottom of the storage cylinder 117, away from the flange 115, with the baffle 114 positioned between the storage cylinder 117 and the extension tube 119. In this embodiment, the outer diameter of the extension cylinder 119 is smaller than the outer diameter of the storage cylinder 117. The filter membrane cylinder 108 is sleeved on the outside of the extension cylinder 119 and is connected to the bottle-shaped base 103. The end of the filter membrane cylinder 108 away from the storage cylinder 117 is covered with a filter membrane 1081 located below the baffle 114. After the rod 205 pushes open the baffle 114, it extends above the filter membrane 1081. The filter membrane 1081 can filter out impurities mixed in the solid culture medium and at the same time can shield the baffle 114 to prevent it from falling into the bottle-shaped base 103. By providing the extension cylinder 119, the baffle 114 is separated from the filter membrane 1081 by a distance, preventing the rod 205 from piercing the filter membrane 1081. Therefore, when the length of the filter membrane cartridge 108 is sufficient, the extension cartridge 119 may be omitted, and the filter membrane cartridge 108 may be directly sleeved onto the bottom of the storage cylinder 117 by means of threaded connection or the like.

[0053] A detachable pull ring 118 is detachably connected to the upper end of the bottle base 103 for retaining the bottle cap 203. In this embodiment, the outer diameter of the bottle body of the bottle base 103 is larger than the outer diameter of the bottle neck, forming a step structure on the bottle base 103. The detachable pull ring 118 is located between the step structure of the bottle base 103 and the bottle cap 203 to support the bottle cap 203 and surround the bottle neck of the bottle base 103, preventing the bottle cap 203 from moving downward. The detachable pull ring 118 is provided with a buckle 1181 for a person to pull. By pulling the buckle 1181 around the bottle mouth of the bottle base 103 and pulling the detachable pull ring 118 outward, the detachable pull ring 118 can be removed, allowing the bottle cap 203 to move toward the bottle body of the bottle base 103. In addition, a sealing assembly 30 may be provided between the bottle mouth of the bottle-shaped base 103 and the bottle cap 203. The sealing assembly 30 includes multiple sealing rings spaced apart from top to bottom to prevent leakage of the liquid culture medium. The specific structure of the sealing assembly 30 may be referred to in Example 3.

[0054] Furthermore, the inner diameter of the storage cylinder 117 gradually decreases from top to bottom, and the outer diameter of the positioning cylinder 116 is smaller than the maximum inner diameter of the storage cylinder 117 and larger than the minimum inner diameter of the storage cylinder 117, so that the positioning cylinder 116 can be interference fit with the storage cylinder 117 after being inserted into the storage cylinder 117, thereby sealing the opening of the storage cylinder 117.

[0055] The bottle cap 203, the storage cylinder 117, and the inner side of the baffle 114 collectively form the solid storage area, while the outer side of the inner support 107 and the inner side of the bottle base 103 collectively form the liquid storage area. After the detachable pull ring 118 is removed, pressing the bottle cap 203 downward pushes the rod 205 out of the baffle 114, establishing communication between the solid storage area and the liquid storage area, allowing the solid culture medium in the inner support 107 to mix with the liquid culture medium in the bottle base 103. In actual use, the liquid culture medium in the bottle base 103 can be added to the level below the filter membrane 1081. After the baffle 114 is opened, the bottle base 103 can be manually shaken to ensure that the solid culture medium fully falls out of the opening in the baffle 114. Alternatively, the bottle base 103 can be inverted and shaken to ensure sufficient contact between the solid and liquid culture media.

[0056] At the same time, after the detachable pull ring 118 is removed, pressing the bottle cap 203 downward can also allow the positioning cylinder 116 to be inserted into the storage cylinder 117 and interference fit with the storage cylinder 117, so that the inner support 107 can be removed from the bottle base 103 together with the bottle cap 203.

[0057] Specific embodiment 2 of the bottle-type culture medium device of the present invention:

[0058] As shown in Figures 5 to 7, the structure of the bottle-type culture medium device is basically the same as that in Example 1, except that: the rod body 205 is located in the inner support 108, and is connected above the baffle 114, extending toward the bottle cap 203, and the slit 1141 surrounds the outer periphery of the rod body 205. The rod body 205 can be integrally formed with the baffle 114, or inserted into the baffle 114. A pressure block 2031 facing the end of the rod body 205 is protruding from the inner top wall of the bottle cap 203. When not in use, the pressure block 2031 is above the rod body 205 and separated by a distance. When in use, as the bottle cap 203 moves downward, it presses down the rod body 205, and the baffle 114 is therefore stressed and separated, thereby achieving communication between the solid storage area and the liquid storage area. Furthermore, the rod body 205 extends upwardly outside the inner support 108, and the positioning cylinder (116) is inserted into the storage cylinder (117) and interference fits therewith for sealing. At this time, the pressing block 2031 can be omitted and replaced by the inner top wall of the bottle cap 203 to press down the rod body 205.

[0059] Furthermore, the filter membrane cartridge 108 provided with the filter membrane may also cover the bottom of the inner support 107 to prevent the blocking piece 114 and the rod body 205 from falling into the bottle-shaped base 103 .

[0060] Furthermore, the opening cover of the storage cylinder 117 facing the bottle cap 203 is provided with a sealing film 1071 made of tin foil or the like. When not in use, the positioning cylinder 116 is located above the top opening of the inner support 107 and is separated by a distance. The sealing film 1071 and the blocking piece 114 respectively seal the upper and lower openings of the inner support 107 to better seal and moisture-proof the solid culture medium located in the inner support 107. During use, as the bottle cap 203 moves downward, the pressing block 2031 and / or the rod body 205 and / or the positioning cylinder 116 approach the sealing film 1071 and pierce it. It is understandable that, since the sealing film 1071 also has a sealing function, the positioning cylinder 116 can be omitted after the sealing film 1071 is provided.

[0061] Specific embodiment 3 of the bottle-type culture medium device of the present invention:

[0062] As shown in Figures 8, 9, and 10, the structure of the bottle-shaped culture medium device is substantially the same as that of Example 1, except that it further includes a base 101 located at the bottom of the bottle-shaped base 103. A clamping sleeve 106 is disposed on the upper end of the base 101, facing the upper end of the bottle-shaped base 103. A placement tray 112 is located within the clamping sleeve 106. The placement tray 112 is slidable in the vertical direction, and the bottle-shaped base 103 is placed on the upper side of the placement tray 112. A plurality of scale lines 104 are provided on the front side of the outer arc circumference of the bottle-shaped base 103, arranged at equal intervals in the vertical direction. A label 105 is affixed to the outer arc circumference of the bottle-shaped base 103. In this embodiment, the bottle-shaped base 103 can be made of a transparent material such as glass or plastic. A fixing assembly 40 for fixing the placement tray 112 is disposed within the clamping barrel 106. A limiting assembly 50 for limiting the position of the bottle cap 203 is disposed at the upper end of the bottle base 103. The sealing assembly 30 is disposed at the upper end of the bottle base 103. In this embodiment, as shown in Figures 10 and 11, the fixing assembly 40 includes two slot frames 110 disposed on the inner circumference of the clamping barrel 106 surrounding the outer side of the bottle base 103. The two slot frames 110 are positioned in corresponding positions on the left and right sides of the clamping barrel 106. The slot frames 110 include a first stopper 1101 and a second stopper 1102. The first stopper 1101 protrudes from the inner circumference of the slot frame 110 and extends along the circumference thereof. A certain distance is provided between the first stopper 1101 and the base 101. The placement tray 112 is located between the first stopper 1101 and the base 101. The second stopper 1102 is located at one end in the direction of extension of the first stopper 1101 and extends downward. Two engaging blocks 109 are provided at the lower end of the outer arc surface of the bottle base 103. Each engaging block 109 enters the slot frame 110 from the end of the second stopper 1102 away from the first stopper 1101, with its upper side abutting the lower side of the first stopper 1101. The placement plate 112 is located between the bottom surface of the bottle base 103 and the top surface of the base 101. The bottle base 103 is rotated until the engaging block 109 abuts the second stopper 1102, thereby achieving mating installation between the engaging block 109 and the adjacent slot frame 110. A mounting spring 111 is provided between the bottom wall of the clamping barrel 106 and the placement plate 112. The mounting spring 111 pushes the placement plate 112 toward the bottle-shaped base 103. Because the first stopper 1101 presses against the clamping block 109, the bottle-shaped base 103 is positioned within the clamping barrel 106. Furthermore, mounting holes 102 are provided at the four corners of the base 101, through which the fixing assembly 40 is secured to a work surface. Furthermore, double-sided tape 113 can be adhered to the underside of the base 101 to secure the fixing assembly 40 in place.

[0063] As shown in Figures 14, 15 and 16, the sealing assembly 30 includes a rectangular sealing ring 305, an arc-shaped sealing ring 306, a trapezoidal sealing ring 307 and a sticking ring 301. The rectangular sealing ring 305, the arc-shaped sealing ring 306 and the trapezoidal sealing ring 307 are made of elastic materials, such as rubber. The rectangular sealing ring 305 is bonded to the outer peripheral surface near the upper end of the bottle mouth of the bottle-shaped base 103, and a rectangular sealing groove 302 is provided on the upper end of the inner arc surface of the bottle cap 203. The rectangular sealing ring 305 is installed in cooperation with the rectangular sealing groove 302. The trapezoidal sealing ring 307 is bonded to the lower end of the bottleneck of the bottle-shaped base 103, and a trapezoidal sealing groove 304 is provided on the lower end of the inner arc surface of the bottle cap 203. The trapezoidal sealing ring 307 is installed in cooperation with the trapezoidal sealing groove 304. The bottle neck of the bottle-shaped base 103 is bonded with an arcuate sealing ring 306 located between the rectangular sealing ring 305 and the trapezoidal sealing ring 307. The inner curved surface of the bottle cap 203 is provided with an arcuate sealing groove 303, and the arcuate sealing ring 306 is mounted in conjunction with the arcuate sealing groove 303. The top wall of the bottle cap 203 is provided with the adhesive ring 301, which is mounted in conjunction with the inner support 107 and bonded to the flange 115 of the inner support 107. The lower end of the bottle cap 203 is provided with a sealing ring 309 bonded to the inner side wall of the lower end of the bottle cap 203. The sealing ring 309 is disposed between the bottle cap 203 and the limiting assembly 50 to seal the gap between the bottle cap 203 and the limiting assembly 50.

[0064] As shown in Figures 12, 13, and 14, the limiting assembly 50 includes a cavity ring 201, a connecting rod 207, a plug post 208, a locking bracket 210, and an adjustment seat 212, disposed on the bottleneck of the bottle base 103. The cavity ring 201 surrounds the outside of the bottleneck of the bottle base 103 and includes a ring body 2011 and a support plate 2012. The ring body 2011 extends along the periphery of the bottleneck of the bottle base 103 and is separated from it by a distance. The support plate 2012 is connected between the ring body 2011 and the bottleneck of the bottle base 103. The support plate 2012 is located on the side of the bottle base 103 that is closest to the bottle body, with the ring body 2011 positioned above it. Furthermore, a docking groove ring 308 is provided on the upward-facing surface of the support plate 2012, and a sealing ring 309 is mounted in conjunction with the docking groove ring 308. Two locking frames 210 are slidably connected within the cavity ring 201 via two pins 208. The two pins 208 are located radially on the front and rear sides of the bottle neck of the bottle-shaped base 103. One end of each pin 208 extends outside the cavity ring 201 and has a stopper for engaging with the cavity ring 201. The outer arc surface of the bottle cap 203 is provided with a socket 206 corresponding to each pin 208, which is configured to engage with the pin 208. A return spring 209 is connected between the cavity ring 201 and each locking frame 210, and is sleeved around the corresponding pin 208. A connecting rod 207 is rotatably connected to each end of each locking frame 210. The two connecting rods 207 on the same locking frame 210 are symmetrical about the return spring 209 connected to the locking frame 210.

[0065] Two adjustment seats 212 are slidably connected within the cavity ring 201 via two sliding posts 213. These two adjustment seats 212 are located on the radially left and right sides of the bottle-shaped base 103. One end of each sliding post 213 extends outside the cavity ring 201 and is connected to a pressure plate 202. The pressing surface of the pressure plate 202 is provided with multiple anti-slip protrusions 211. Two connecting rods 207, rotatably connected to the same locking frame 210, have their ends, distal from the locking frame 210, rotatably connected to corresponding ends of the two adjustment seats 212.

[0066] The operator presses the pressing plates 202 on both sides, and the anti-skid protrusions 211 on the pressing plates 202 can prevent the hands from slipping on the pressing plates 202. Then, the sliding columns 213 drive the adjustment seats 212 on the left and right sides to move closer together, so that the connecting rods 207 inside the adjustment seats 212 rotate, thereby driving the front and rear locking frames 210 to move away from each other under the restriction of the plug posts 208, so that the plug posts 208 leave the inside of the insertion holes 206. At this time, the return spring 209 is in a compressed state, and then the operator removes the bottle cap 203 from the bottle base 103 upwards. The anti-skid protrusions 204 on the bottle cap 203 can prevent the operator from slipping. Then, the personnel takes out the inner tray 107 and puts the solid culture medium into it, and then puts the liquid culture medium into the bottle-shaped base 103. The precise pouring can be achieved by observing the scale line 104. Then, the inner tray 107 filled with solid culture medium is placed into the bottle-shaped base 103, and then the bottle cap 203 is placed on the upper end of the bottle-shaped base 103. The bottle cap 203 is then pressed downward so that the socket 206 is aligned with the pin 208. Then, the personnel releases the pressing plate 202. At this time, the reset spring 209 rebounds quickly to drive the pin 208 on the locking frame 210 to be inserted into the socket 206 to complete the assembly of the bottle cap 203 and the bottle-shaped base 103.

[0067] During transportation or placement, the rectangular sealing ring 305 contacts the rectangular sealing groove 302 to prevent the liquid culture medium from overflowing, thereby realizing the first layer of sealing. The arc-shaped sealing ring 306 is squeezed into the inside of the arc-shaped sealing groove 303 to form a second layer of sealing. The trapezoidal sealing ring 307 cooperates with the trapezoidal sealing groove 304 to realize the third layer of sealing. The three-layer sealing between the bottle cap 203 and the bottle base 103 is realized by sealing rings of different shapes. The sealing ring 309 at the lower end of the bottle cap 203 and the docking groove ring 308 realize the sealing between the bottle cap 203 and the cavity ring 201. It can be understood that the upper and lower order of the rectangular sealing ring 305, the arc-shaped sealing ring 306 and the trapezoidal sealing ring 307 can be exchanged according to different situations. In addition, the rectangular sealing ring 305, the arc-shaped sealing ring 306 and the trapezoidal sealing ring 307 can also be set on the inner side wall of the bottle cap 203, and the rectangular sealing groove 302, the arc-shaped sealing ring 306 and the trapezoidal sealing groove 304 are correspondingly opened on the outer peripheral surface of the bottleneck of the bottle-shaped base 103.

[0068] When in use, the personnel first fix the base 101 in a suitable position, and then press the pressing plates 202 on both sides, and then drive the adjustment seats 212 on the left and right sides to move closer together through the sliding column 213, so that the connecting rod 207 inside the adjustment seat 212 rotates, and then drives the front and rear locking frames 210 to move away from each other under the restriction of the plug column 208, so that the plug column 208 leaves the inside of the socket 206, and then continues to press the bottle cap 203 so that the sealing ring 309 and the docking groove ring 308 are squeezed, so that the rod body 205 pierces the blocking piece 114 of the inner support 107, and at this time the adhesive ring 301 It contacts the flange 115 of the inner support 107, and the solid culture medium in the inner support 107 is filtered by the filter membrane cartridge 108 and mixed with the liquid culture medium. The filter membrane cartridge 108 can prevent solid particles with a pore size of more than 400um from passing through, thereby preventing some impurities from falling into the bottle-shaped base 103 during mixing. Then the personnel take out the bottle cap 203, and the adhesive ring 301 inside the bottle cap 203 sticks the inner support 107 and the filter membrane cartridge 108 together, and is taken out together with the bottle cap 203, which is convenient for personnel to use and avoids contamination of the mixed culture solution.

[0069] After use, the person aligns the bottle base 103 with the placement tray 112 and presses it downward, causing the placement tray 112 to move downward. At this time, the mounting spring 111 is compressed, and then the person rotates the bottle base 103 so that the clamping block 109 rotates to the inside of the card slot rack 110. Then, the person lets go, and at this time, the mounting spring 111 quickly rebounds and drives the bottle base 103 on the placement tray 112 to move upward, causing the clamping block 109 to abut against the card slot rack 110, thereby fixing the bottle base 103, preventing the bottle base 103 from being hit and affecting the use of the person, and making it convenient for the person to take and put the bottle base 103 at any time.

[0070] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention and are not limiting. Other modifications or equivalent substitutions made to the technical solution of the present invention by ordinary technicians in this field should be included in the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solution of the present invention.

Claims

1. A bottle-type culture medium device, characterized in that: The invention comprises a bottle-shaped base (103), an inner support (107) and a bottle cap (203); the inner support (107) is installed on the bottle mouth of the bottle-shaped base (103) and divides the internal space of the bottle-shaped base (103) into a solid storage area located inside the inner support (107) and a liquid storage area located outside the inner support (107); the bottle cap (203) is covered on the bottle mouth of the bottle-shaped base (103); in a use state, the solid storage area is connected with the liquid storage area.

2. The bottle-type culture medium device according to claim 1, characterized in that: The inner support (107) comprises a storage cylinder (117) and a blocking piece (114); the storage cylinder (117) is detachably mounted on the bottle mouth of the bottle-shaped base (103) and extends toward the inside of the bottle-shaped base (103); the blocking piece (114) covers the bottle mouth of the storage cylinder (117) at one end away from the bottle mouth of the bottle-shaped base (103).

3. The bottle-type culture medium device according to claim 2, characterized in that: A rod body (205) extending downward is protrudingly provided on the inner top wall of the bottle cap (203), and the lower end of the rod body (205) extends out of the bottle cap (203); in a use state, the bottle cap (203) moves downward, and the rod body (205) can push open the blocking piece (114), thereby connecting the solid storage area with the liquid storage area.

4. The bottle-type culture medium device according to claim 3, characterized in that: A positioning tube (116) is also protrudingly provided on the inner top wall of the bottle cap (203) and surrounds the outer circumference of the rod body (205). The positioning tube (116) can be inserted into the storage cylinder (117) and has an interference fit therewith.

5. The bottle-type culture medium device according to claim 3, characterized in that: It also includes a detachable pull ring (118), which is located between the bottle-shaped base (103) and the bottle cap (203) to limit the downward movement of the bottle cap (203).

6. The bottle-type culture medium device according to claim 2, characterized in that: The inner support (107) further comprises a filter membrane cartridge (108), wherein the filter membrane cartridge (108) is sleeved on the bottom of the storage cylinder (117) and is covered with a filter membrane (1081) on a side away from the storage cylinder (117), wherein the filter membrane (1081) is located below the baffle (114).

7. The bottle-type culture medium device according to claim 4, characterized in that: The inner diameter of the storage cylinder (117) gradually decreases from top to bottom, and the outer diameter of the positioning cylinder (116) is smaller than the maximum inner diameter of the storage cylinder (117) and larger than the minimum inner diameter of the storage cylinder (117).

8. The bottle-type culture medium device according to claim 2, characterized in that: The baffle (114) is provided with a rod (205) extending upward; when in use, the bottle cap (203) moves downward, the rod (205) is pressed downward and can push open the baffle (114), thereby connecting the solid storage area with the liquid storage area.

9. The bottle-type culture medium device according to claim 8, characterized in that: A pressing block (2031) facing the rod body (205) is protrudingly provided on the inner top wall of the bottle cap (203), and the pressing block (2031) is capable of pressing down the rod body (205).

10. The bottle-type culture medium device according to claim 8, characterized in that: A positioning tube (116) is protrudingly provided on the inner top wall of the bottle cap (203), and the positioning tube (116) can be inserted into the storage cylinder (117) and have an interference fit therewith.

11. The bottle-type culture medium device according to claim 8, characterized in that: The opening of the storage cylinder (117) facing the bottle cap (203) is provided with a sealing film (1071).

12. The bottle-type culture medium device according to claim 11, characterized in that: A positioning tube (116) is protrudingly provided on the inner top wall of the bottle cap (203), and the positioning tube (116) is located above the sealing film (1071) and separated by a distance; in a use state, the bottle cap (203) moves downward, and the positioning tube (116) presses down the sealing film (1071).

13. [Corrected 06.06.2024 according to Rule 26] The bottle-type culture medium device according to claim 1, characterized in that: The top of the inner support (107) is provided with a flange (115) for engaging with the bottle-shaped base (103) for blocking.

14. [Corrected 06.06.2024 according to Rule 26] A bottle-type culture medium device according to claim 1, characterized in that: A base (101) is provided at the bottom of the bottle-shaped base, a clamping cylinder (106) is provided at the upper end of the base (101), a placement plate (112) which slides in the up-down direction is provided inside the clamping cylinder (106), and a fixing assembly (40) for fixing the bottle-shaped base (103) is provided inside the clamping cylinder (106).

15. A bottle-type culture medium device according to claim 14, characterized in that: The fixing assembly (40) comprises two slot frames (110) arranged on the inner arc wall of the clamping tube (106), the two slot frames (110) are located at corresponding left and right positions, and the slot frames (110) are located above the placement plate (112); two clamping blocks (109) are arranged at the lower end of the outer arc circumference of the bottle-shaped base (103), and the clamping blocks (109) are respectively inserted into the corresponding slot frames (110), and a mounting spring (111) is arranged in the clamping tube (106), and the two ends of the spring are respectively connected to the placement plate (112) and the base (101).

16. A bottle-type culture medium device according to claim 1, characterized in that: The bottle cap (203) further comprises a limit assembly (50), wherein the limit assembly (50) comprises a cavity ring (201), a connecting rod (207), a plug post (208), a locking frame (210) and an adjusting seat (212) arranged at the upper end of the bottle-shaped base (103); two locking frames (210) are slidably connected in the cavity ring (201) through two plug posts (208), respectively, and the two plug posts (208) are respectively located at the radial front and rear sides of the bottle-shaped base (103); one end of the plug post (208) passes through the cavity ring (201) and is connected to a stopper for engaging with the cavity ring (201); a plug hole (206) for engaging with the plug post (208) is provided at a position corresponding to each plug post (208) on the outer arc circumference of the bottle cap (203); and a multiple locking member (212) is connected between the cavity ring (201) and each locking frame (210). A reset spring (209) is provided, and the reset spring (209) is sleeved outside the corresponding plug column (208); the two ends of each locking frame (210) are rotatably connected with a connecting rod (207), and the two connecting rods (207) located on the same locking frame (210) are symmetrical about the reset spring (209) connected to the locking frame (210); two adjustment seats (212) are slidably connected in the cavity ring (201) through two sliding columns (213), and the two adjustment seats (212) are respectively located on the radial left and right sides of the bottle-shaped base (103), and one end of the sliding column (213) extends out of the cavity ring (201) and is connected to a pressing plate (202); the ends of the two connecting rods (207) rotatably connected to the same locking frame (210) and away from the locking frame (210) are rotatably connected to the corresponding ends of the two adjustment seats (212).

17. A bottle-type culture medium device according to claim 1, characterized in that: A plurality of anti-slip protrusions (204) are arranged on the outer peripheral surface of the bottle cap (203).

18. A bottle-type culture medium device according to claim 1, characterized in that: The invention also comprises a sealing assembly (30), wherein the sealing assembly (30) comprises at least one of a rectangular sealing ring (305), an arcuate sealing ring (306) and a trapezoidal sealing groove (304) protruding from the outer peripheral wall of the bottleneck of the bottle-shaped base (103) or the inner peripheral wall of the bottle cap (203), and the inner peripheral wall of the bottle cap (203) or the outer peripheral wall of the bottleneck of the bottle-shaped base (103) is respectively provided with a rectangular sealing groove (302), an arcuate sealing groove (303) and a trapezoidal sealing groove (304) corresponding thereto.

19. A bottle-type culture medium device according to claim 18, characterized in that: The sealing component (30) is provided as a sealing ring (309) between the bottle cap (203) and the limiting component (50).

20. The bottle-type culture medium device according to claim 14, characterized in that: The four corners of the base (101) are respectively provided with mounting holes (102), or the lower end of the base (101) is bonded with a double-sided adhesive tape (113).

21. The bottle-type culture medium device according to claim 1, characterized in that: A label (105) is attached to the outer peripheral surface of the bottle-shaped base (103).

Images