Process for obtaining nanostructures comprising curcuma longa; nanostructures of c. longa; compositions comprising nanostructures of c. longa

Abstract

The present invention relates to the process of obtaining nanostructures comprising (a) Curcuma longa (turmeric) oil and / or extract, (b) at least one surfactant, (c) water and (d) optionally at least one organic solvent. The present invention also relates to the nanostructures obtained by this process, which are low-energy methods comprising extracts and / or oils from C. longa. The present invention also relates to cosmetic compositions for skin, hair and nail care.

Description

DESCRIPTION PROCESS OF OBTAINING NANOSTRUCTURES COMPRISING Curcuma longa, NANOSTRUCTURES OF C. longa; COMPOSITIONS COMPRISING THE NANOSTRUCTURES OF C. longa Fundamentals of the invention

[0001] Curcuma longa is a plant belonging to the Zingiberaceae family. The most commonly used part of the plant is the rhizome. When dried and ground, a yellow-orange powder is obtained, commonly used in cooking as a seasoning and natural colorant (AMMON; WAHL, 1991; BARANKEVICZ, 2015; CARVALHO, 2014; ESATBEYOGLU et al., 2012).

[0002] The constituent substances are polysaccharides, mineral salts, essential oils, and polyphenols. The polyphenols present are called curcuminoids, responsible for its therapeutic action. They confer the characteristic yellow-orange coloration of this species. The three main curcuminoids are: curcumin, demethoxycurcumin, and bisdemethoxycurcumin. They represent 3-15% of the rhizome components, with curcumin being the main active compound, whose concentration can vary from 1.5 to 7.1% (ESATBEYOGLU et al., 2012; NAIR, 2019; YIXUAN et al., 2021).

[0003] Curcuminoids have a unique conjugated structure including two methoxylated phenols and a p-diketone in the form of an enol, demonstrating their ability to capture oxidative species and are therefore used as an antioxidant (CHANDA; RAMACHANDRA, 2019; MASUDA et al., 2001; NAIR, 2019; SAAD et al., 2018; SHUKLA; LAD, 2022).

[0004] C. longa is a plant classified as safe by the U.S. Food and Drug Administration (FDA) and the World Health Organization (WHO), and its medicinal use is even recommended. It offers several pharmacological benefits, proven in the literature, such as antimicrobial, antiparasitic, anti-inflammatory, and antioxidant properties (SAAD et al., 2018; WHO, 1999).

[0005] In hair formulations, some studies show the use of C. longa extract in hair growth treatments. Patents have been filed for formulations for this purpose (MORLEY, 2020). The company Chemyunion Química Ltda. filed a patent with the National Institute of Industrial Property (INPI) using C. longa extract as a golden and / or orange shine toner and as a photoprotector for natural and dyed hair (BARRERA-ARELLANO et al., 2005).

[0006] Some cosmetic products containing C. longa are already on the market, but they are focused on oral hygiene and hair care. However, given C. longa's organoleptic characteristics (yellow color and characteristic odor), its use in skin care cosmetics is still limited, as it primarily leaves the skin yellow (SARAF, 2011). With its ability to mask undesirable organoleptic characteristics, nanobiotechnology can be used to solve this problem.

[0007] Nanobiotechnology involves the production, manipulation, and use of materials ranging in size from smaller than a micrometer (1000 nm) down to individual atoms, and is used not only in chemical approaches but also in biological materials (SAGLAM; KORKUSUZ; PRASAD, 2021). Size reduction in materials to the nanometer scale (1 nm = 10' 9 m) generates changes in its physical, chemical and biological properties, resulting in new applications in various segments (ALEXIS et al., 2008; KIM; RUTKA; CHAN, 2010; SAGLAM; KORKUSUZ; PRASAD, 2021; SOUZA, 2012).

[0008] In cosmetics, the use of nanobiotechnology allows for increased effectiveness of products, due to the gradual release of the active ingredient and increased contact time with the surface; increased permeation of the constituent active ingredients; increased stability of the active ingredients; protection of the active ingredients against degradation of both the formulation and the environment; reduction of odors, irritations and unwanted colorations at the application site; and improvement in the natural resistance of the skin / lips / nails / hair, helping to repair and strengthen deeper layers.

[0009] Nanobiotechnology has been receiving special attention for the incorporation of naturally occurring bioactive ingredients in cosmetology. Harris et al. (2011) produced chitosan nanoparticles containing natural antioxidants extracted from Ilex paraguariensis, which can be applied in cosmetics. Matos (2014) developed a topical formulation containing chitosan nanoparticles as a strategy to increase the follicular penetration of minoxidil sulfate for the treatment of androgenic alopecia.

[0010] However, the production methods used are considered high-energy. High-energy nanostructuring methods use high amounts of mechanical energy to disrupt structures, separating droplets or particles to the nanometer scale. Expensive and sophisticated equipment capable of producing high mechanical energy is used to achieve this, such as ultrasonic homogenizers, microfluidizers, ultrasonicators, ultraturrax, sonicators, ultrasonic baths, high-shear homogenizers, high-pressure homogenizers, etc. Nanostructures are formed by these methods through the following principle: the greater the energy input, the smaller the droplet size. However, the energy required to obtain nanometer-scale droplets is very high and, therefore, inefficient in terms of cost and energy.Especially considering that only a small amount of the energy produced is used (about 0.1%). Because of this, these methods are not considered environmentally sustainable (GRAVES et al., 2005).

[0011] Articles and patents containing C. longa nanostructures were found. Bhawana et al. (2011) obtained C. longa nanoparticles by solubilizing them in dichloromethane, a carcinogenic solvent, which was obtained through a high-energy input technique using ultrasonication. Zamarioli et al. (2015) obtained solid lipid nanoparticles through the emulsion technique using a high-shear homogenizer. And, Araya-Sibaja et al. (2022) obtained bovine serum albumin-based nanoparticles comprising C. longa through an ultraturrax.

[0012] Document WO 2020109989 (A1) described compositions and processes for producing solid lipid nanoparticles containing curcumin. The inventors developed nanoparticles through high-energy homogenization, whose compositions necessarily contained solid lipids, surfactants, and co-solvents to promote high encapsulation efficiency. Furthermore, several process steps were required, including the use of temperature elevation and reduction, increasing production time and reducing industrial scalability. Furthermore, it is reported that the resulting nanoparticles can stain the skin.

[0013] Document BR 102021002585-9 A2 described compositions and processes for obtaining gelled nanoparticles from microemulsions intended to be a drug carrier in which a gradual increase and reduction in temperature is used (melting temperature of the organogelling agent and then cooling to a temperature between -4°C and 30°C) for the formation of nanoparticles or the use of high energy input through agitation with ultraturrax.

[0014] All the examples cited do not conflict with the present invention, as they used high-energy techniques using equipment such as ultrasonic and ultraturrax, including non-green solvents, i.e., the use of toxic and / or environmentally harmful solvents, such as dichloromethane and methanol, and also the use of polymers and proteins, such as albumin. Furthermore, they did not prevent skin staining, making them unsuitable for use in skin care cosmetics.

[0015] The process developed in this invention uses the low energy input technique in which the internal chemical potential of the solution is used, requiring low energy demand.

[0016] Thus, contrary to what was found, no process or formulation components are similar to that described in this present application. This is because the present invention created a new method for producing nanostructures comprising C. longa through a low-energy technique that uses only green solvents, such as ethanol and water, and has a low energy demand (not requiring several process steps).

[0017] The technique developed does not require traditional nanostructure production equipment such as ultrasonics, ultraturrax, sonicators, ultrasonic baths, high-shear homogenizers, etc., as used in the articles and patents found. Thus, it requires less energy in the process to generate nanostructures. This eliminates energy waste and is environmentally sustainable. Furthermore, unlike previous findings, polymers and / or metals are not used to obtain the nanostructures. Thus, a green process was developed that generates a new nanostructure comprising C. longa oil and / or extract that is non-toxic and considered green, producing sustainable, vegan, scalable, and skin-friendly C. longa nanostructures. This enables easy preparation, reproducibility, industrial scalability, and use in skin care cosmetics.

[0018] Therefore, among the searches carried out in the literature as well as in patent banks, no technique, process or formulation components are similar to that described in this present application. Brief description of the invention

[0019] The present invention relates to a new process for producing nanostructures comprising C. longa.

[0020] The present invention also relates to the process of producing nanostructures comprising extracts and / or oils from C. longa using a novel low-energy method. The low-energy nanostructuring method described in this invention uses low amounts of energy to produce the nanostructures, making it more energy-efficient and environmentally friendly. In addition, Furthermore, it does not require sophisticated equipment, such as ultrasonicators, ultraturrax or other high shear / agitation / sonication equipment, thus reducing the cost of producing nanostructures.

[0021] The composition of nanostructures obtained by this invention comprises a combination of at least three components: (a) oil and / or extract of Curcuma longa, (b) at least one surfactant and (c) water;

[0022] The composition of nanostructures obtained by this invention may also optionally comprise other components such as preservatives, fragrances, aromas, perfumes, flavorings and thickeners.

[0023] The nanostructures obtained in the present invention can function as a cosmetic composition and / or cosmeceutical composition for skin, hair and nail care.

[0024] The present invention also relates to the cosmetic composition comprising the nanostructures obtained by this invention.

[0025] Surprisingly, the inventors succeeded in developing a process for producing nanostructures containing C. longa using a previously unproven low-energy method. This resulted in stable C. longa nanostructures that showcase all the advantages of nanobiotechnology and the benefits of C. longa. Furthermore, the resulting C. longa nanostructures do not transfer their yellow color to skin, hair, and nails. Thus, these nanostructures can be used in cosmetic formulations for a variety of uses, including products for the face and body.

[0026] Thus, they have the following advantages over the processes and / or products found on the market and / or in the literature: a) Comprising the use of a natural active ingredient (C. longa) associated with high-standard technology (nanobiotechnology) b) Process comprising the low-energy technique for the production of nanostructures comprising oil and / or extract of C. longa in which moderate agitation is used to mix the organic phase (FO) in the aqueous phase (FA) or vice versa, being economically and environmentally more sustainable than traditional methods c) Comprising the non-use of any raw material of animal origin (vegan product) d) Comprising nanostructures comprising oil and / or extract of C. longa produced by the process described in the present invention characterized by not staining the skin.

[0027] Other features and advantages of the present invention will become apparent from the following more detailed description of desirable embodiments which illustrate, by way of example, the principles of the present invention. Brief description of the drawings

[0028] The invention will be described in a preferred embodiment. Therefore, for better understanding, references will be made to the following figures: Figure 1 presents a particle size graph of a nanostructure comprising C. longa oil and / or extract produced according to the present invention superimposing the readings taken on day zero, day 7 and day 30, showing that they are in the nanometric scale and that they are stable; Figure 2 shows a photo of a forearm containing the application of Curcuma longa oil and / or extract (A), the nanostructure solution comprising C. longa oil and / or extract produced by the process described in the present invention (B), a cosmetic formulation containing the nanostructure comprising oil and / or extract of C. longa described in the present invention (C) on the skin demonstrating the masking of the yellow color of C. longa by the nanostructures produced by the present invention, both in solution and in a cosmetic formulation;

[0029] It should be understood that the various aspects are not limited to the arrangements and instrumentality shown in the drawings. Summary of the invention

[0030] Broadly speaking, the present process for obtaining nanostructures comprising C. longa by a low-energy input method comprises the following steps:

[0031] a) From the processed C. longa plant (harvested, washed, sliced, dried and crushed) obtain C. longa extract and / or oil,

[0032] b) Phase I: formation of C. longa nanostructures obtained by agitation of the oil and / or C. longa extract, internal constituents, comprising or not one or more surfactants, comprising or not an organic solvent in the aqueous dispersion medium;

[0033] c) Phase II: evaporation of the organic solvent using solvent evaporation equipment. This phase is not mandatory;

[0034] Nanostructures comprising C. longa oil and / or extract are obtained by means of the process described in this present invention.

[0035] Cosmetic formulations use nanostructures comprising C. longa oil and / or extract obtained by the process described in the present invention. Detailed description of the invention Production of nanostructures comprising C. longa oil and / or extract

[0036] The first object of invention relates to the process of producing nanostructures comprising oil and / or extract of C. longa by a low energy input method developed.

[0037] The process of the invention typically includes: i. Processing the C. longa plant (harvesting, washing, drying, slicing and grinding the plant material); ii. Obtaining the C. longa extract and / or oil, iii. Preparing the organic phase (OP) by mixing the C. longa extract and / or oil with at least one surfactant until completely homogenized; iv. At least one organic solvent may also be added to dilute the C. longa extract and / or oil being added to the OP (step iii); v. Preparing the aqueous phase (AP) composed of water; vi. The OP and / or AP may comprise the presence of at least one preservative and / or at least one thickener and / or at least one fragrance / aroma / flavoring / perfume that will be added in the formation of the OP (step iii) and / or AP (step v) depending on their solubility characteristics, with constant stirring until completely homogenized. This step is not mandatory; vii.Gradually add the FA (step v+vi) to the FO (steps iii+iv+vi) with constant stirring in low-energy stirrers (between 200 rpm and 10,000 rpm) until the FA is completely added (step v) and completely homogenized. The process can also occur in reverse, that is, slowly adding the FO (steps iii+iv+vi) to the FA (step v+vi). Constant stirring until the FO is fully added (steps iii, iv, and vi) and completely homogenized; viii. Evaporate the organic solvent using solvent evaporation processes such as rotary evaporators, ovens, natural evaporation, and exhaust fans. This step is not mandatory.

[0038] The processing of the plant material of C. longa, which mainly comprises the rhizomes although the other parts of the plant are equally useful (leaves, flowers and stems) is done as follows: the plant material is washed, sliced, dried in an oven at a temperature between 15 and 100°C or dried at room temperature, followed by grinding in a mill until it reaches a particle size between 50 and 800 pm.

[0039] The oil is obtained by hydrodistillation or steam distillation.

[0040] The extract is obtained by maceration and / or percolation processes and their combinations using at least one organic solvent.

[0041] The following organic solvents may be used in the present invention for the process of obtaining C. longa extract: water, alcohols, ketones, esters, ethers, hydrocarbons, and mixtures thereof. Examples include, but are not limited to, water, methanol, ethanol, propanol, ethyl acetate, isopropyl acetate, acetone, propylene glycol, cyclohexanone, and ethyl acetate.

[0042] The C. longa extract obtained can be liquid, soft or dry, which differ in the evaporation time in which it allows the greatest concentration of total solids between liquid, soft and dry, respectively.

[0043] To ensure the curcuminoid content in oils / extracts, especially curcumin, it can be quantified by High Performance Liquid Chromatography (HPLC) after analytical validation in accordance with current Brazilian legislation. Curcumin levels can range from 2% to 30%.

[0044] An example of the curcumin levels obtained by HPLC are shown in Table 1. The standard deviation (SD) and relative standard deviation (RSD) values ​​were less than 5%, indicating the repeatability of the method. TABLE 1: Examples of curcumin levels by HPLC.

[0045] The organic phase (OP) for the production of C. longa nanostructures by the low-energy input method consists of C. longa extract and / or oil between 0.01 and 20% by weight, and at least one surfactant between 0.01 and 20% by weight. An organic solvent can be added to dilute the C. longa extract and / or oil between 0.1 and 20% by weight.

[0046] The aqueous phase (AF) was composed of water between 60 and 99.99% by weight.

[0047] The FO and / or FA may additionally comprise between 0.001 and 5% of at least one preservative and / or at least one fragrance / aroma / flavoring / perfume.

[0048] The FO and / or FA may also additionally comprise between 0.1 and 10% of at least one thickener.

[0049] The homogenization process described in step (vi) can be carried out at a temperature between 4 and 50 °C with constant stirring until the components are fully added and until complete homogenization.

[0050] Thus, the present invention provides a process for obtaining nanostructures comprising oil and / or extract of C. longa that is easy to apply and low cost.

[0051] The invention also provides a process for obtaining nanostructures comprising C. longa oil and / or extract that is environmentally sustainable by using green solvents and green chemistry.

[0052] The invention also provides a process for obtaining nanostructures comprising oil and / or extract of C. longa that allows the formation of nanostructures on a nanometric scale (between 1 and 1000nm).

[0053] The invention also provides a process for obtaining nanostructures comprising C. longa oil and / or extract that provides analytical control over the content of active compounds.

[0054] The invention also provides a process for obtaining nanostructures comprising C. longa oil and / or extract at a low cost and is economically sustainable. Surfactants

[0055] At least one surfactant used for the production of C. longa nanostructures is selected from the group of ionic surfactants, nonionic surfactants and mixtures thereof. Preferably, the amount of total surfactant used in the present invention ranges from about 0.01% to about 20% by weight and preferably from about 0.01% to about 15% by weight, more preferably from about 0.01% to about 10% by weight, based on the total weight of the composition. Ionic surfactants

[0056] Non-limiting examples of ionic surfactants comprising anionic and cationic surfactants that may be used in the present invention are selected from the group comprising alkyl sulfates, alkyl phosphates, alkyl ether sulfates, alkyl ether phosphates, alkyl starch ether sulfates, alkyl aryl polyether sulfates, monoglyceride sulfates, sulfonates such as alkyl sulfonates, alkyl amide sulfonates, alkyl aryl sulfonates, alpha-olefin sulfonates, paraffin sulfonates, sulfosuccinates, alkyl sulfosuccinates, alkyl ether sulfosuccinates, alkyl amide sulfosuccinates, alkyl sulfoacetates, acyl sarcosinates, acyl glutamates, acyl sulfosuccinamates, and so on. alkyl, N-acyl taurates and M-methyltaurates, isethionates, N-acyl isethionates, N-acyl taurates, alkyl phosphates and phosphates, salts of alkyl monoesters and polyglycoside-polycarboxylic acids, acyl lactylates,salts of D-galactoside uronic acids, salts of alkyl ether carboxylic acids, salts of alkyl aryl ether carboxylic acids, and salts of alkyl amido ether carboxylic acids; or the unsalified forms of all these compounds, wherein the alkyl and acyl groups of all these compounds contain from 6 to 24 carbon atoms and the aryl group denotes a phenyl group. Some of these compounds may be oxyethylenated and then preferably comprise from 1 to 50 ethylene oxide units.

[0057] The ionic surfactant can also be chosen, in particular, from anionic derivatives of plant-based proteins or silk proteins, phosphates and alkyl phosphates, carboxylates, sulfosuccinates, amino acid derivatives, alkyl sulfates, alkyl ether sulfates, sulfonates, isethionates, taurates, alkyl sulfoacetates, polypeptides, anionic derivatives of alkyl polyglucosides, and mixtures thereof. a) Ionic derivatives of plant-based proteins are hydrolysates of proteins containing a hydrophobic group. This hydrophobic group may be naturally present in the protein or may be added by reacting the protein and / or protein hydrolysate with a hydrophobic compound. The proteins are plant-based or silk-derived, and the hydrophobic group may be, in particular, a fatty chain, for example, an alkyl chain containing 10 to 22 carbon atoms. They can be cited, more particularly made,as anionic derivatives of proteins of vegetable origin, apple, wheat, soy, or oat protein hydrolysates, comprising an alkyl chain having 10 to 22 carbon atoms, and their salts. The alkyl chain may, in particular, be a lauryl chain and the salt may be a sodium, potassium, and / or ammonium salt. b) Mention may be made as phosphates and alkyl phosphates, for example, of monoalkyl phosphates and dialkyl phosphates, such as lauryl monophosphate, such as the product sold under the name MAP20® by Kao Chemicals, the potassium salt of dodecyl phosphate, the mixture of mono- and diesters (predominantly diester), for example, sold under the name Crafol AP-31® by Cognis, the mixture of monoester and diester of octyl phosphate, such as the product sold under the name Crafol AP-20® by Cognis, the mixture of monoester and diester of 2-butyloctyl phosphate ethoxylate (7 mol EO), such as the product sold under the name Isofol 12 7 EO-Phosphate Ester® by Condea,the potassium or triethanolamine salt of mono(C12-C13) alkyl phosphate, such as the products sold under the references Arlatone MAP230K-40® and Arlatone MAP 230T-60® by Uniqema, potassium lauryl phosphate, such as the product sold under the name Dermalcare MAP XC-99 / 09R) by Rhodia Chimie, and potassium cetyl phosphate, for example, sold under the name Arlatone MAP 160K by Uniqema. c) Mention may be made as carboxylates of: starch ether carboxylates (AEC), such as sodium lauryl starch ether carboxylate (3 EO), for example, sold under the name Akypo Foam 30® by Kao Chemicals; polyoxyethylenated carboxylic acid salts, such as oxyethylenated sodium lauryl ether carboxylate (6 EO) (65 / 25 / 10 C12-C14-C16), for example, sold under the name Akypo Soft 45 NV® by Kao Chemicals, polyoxyethylenated and carboxymethylated fatty acids from olive oil, such as that sold under the name Olivem 400® by Biologia E Tecnologia, or oxyethylenated sodium tridecyl ether carboxylate (6 EO),for example, marketed under the name Nikkol ECTD-6NEX® by Nikkol; and salts of fatty acids (soaps) with a C6 to C22 alkyl chain that are neutralized with an organic or inorganic base, such as potassium hydroxide, sodium hydroxide, triethanolamine, N-methylglucamine, lysine and arginine. d) Mention may be made, in particular, as amino acid derivatives, of alkaline salts of amino acids, such as: sarcosinates, such as sodium lauroyl sarcosinate, for example, sold under the name Sarkosyl NL 97® by Ciba or sold under the name Oramix L 30® by Seppic, sodium myristoyl sarcosinate, such as the product sold under the name Nikkol Sarcosinate MN® by Nikkol, or sodium palmitoyl sarcosinate, sold under the name Nikkol Sarcosinate PN® by Nikkol; alaninates, such as N-lauroyl-N-methyl sodium amidopropionate, for example, sold under the name of Nikkol Sodium alanine LN 30® by Nikkol or sold under the name of Alanone ALE® by Kawaken, or triethanolamine N-lauroylN-methylalanine, for example, sold under the name of ALTA® for Kawaken; glutamates, such as triethanolamine monococoil glutamate, such as the product sold under the name Acylglutamate CT-12® by Ajinomoto, triethanolamine lauroyl glutamate, for example, such as the product sold under the name acylglutamate LT-12® by Ajinomoto, this glutamate glutamate; aspartates, such as the mixture of triethanolamine N-lauroyl aspartate and triethanolamine N-myristoyl aspartate, for example, sold under the name Asparack® by Mitsubishi; glycine derivatives (glycinates),such as sodium N-cocoyl glycinate, for example, sold under the names Amilite GCS-12® and Amilite GCK 12 by Ajinomoto; citrates, such as the citric monoester of oxyethylenated coco alcohols (9 mol), sold under the name Witconol EC 1129 by Goldschmidt; and galacturonates, such as sodium dodecyl D-galactoside uronate, sold by Soliance. e) Mention may be made as sulfosuccinates, for example, oxyethylenated lauryl alcohol monosulfosuccinate (3 EO) (70 / 30 C12 / C14), such as the product sold under the names Setacin 103 Special® and Rewopol SB-FA 30 K 4 ® by Witco, the disodium salt of a hemisulfosuccinate of C12-C14 alcohols, for example, sold under the name Setacin F Special Paste® by Zschimmer Schwarz, oxyethylenated disodium oleamidosulfosuccinate (2 EO), such as the product sold under the name Standapol SH 135® by Cognis, oxyethylenated lauramide monosulfosuccinate (5 EO), for example, sold under the name Lebon a-5000® by Sanyo,the disodium salt of oxyethylenated lauryl citrate monosulfosuccinate (10 OE), for example, sold under the name Rewopol SBCS 50® from Witco or ricinoleic monoethanolamide monosulfosuccinate, for example, sold under the name Rewoderm S 1333® from Witco. Polydimethylsiloxane sulfosuccinates, such as PEG-12 disodium dimethicone sulfosuccinate, for example, sold under the name Mackanate-DC 30 from MacIntyre, may also be used. f) Mention may be made as alkyl sulfates, for example, of triethanolamine lauryl sulfate (CTFA name: TEA lauryl sulfate), such as the product sold by Huntsman under the name Empicol TL40 FL or the product sold by Cognis under the name Texapon T42, which products are 40% in aqueous solution. Mention may also be made of ammonium lauryl sulfate (CTFA name: ammonium lauryl sulfate), such as the product sold by Huntsman under the name Empicol AL 30FL, which is 30% in aqueous solution. g) Mention may be made, as alkyl ether sulfates, for example,sodium lauryl ether sulfate (CTFA name: sodium laureth sulfate), such as that sold under the names Texapon N40 and Texapon AOS 225 UP from Cognis, or ammonium ... CTFA: ammonium laureth sulfate), such as that sold under the name Standapol EA-2 by Cognis. h) Mention may be made as sulfonates, for example, of sodium olefin sulfonates, such as sodium α-olefin sulfonate (C14-C16), such as the product sold under the name Bio-Terge AS-40® by Stepan, sold under the names Witconate AOS Protégé® and Sulframine AOS PH 12® by Witco or sold under the name Bio-Terge AS-40 CG® by Stepan, secondary sodium olefin sulfonate, for example, sold under the name Hostapur SAS 30® by Clariant; or linear alkylarylsulfonates, such as sodium xylenesulfonate, for example, sold under the names Manrosol SXS30®, Manrosol SXS40® and Manrosol SXS93® by Manro. i) Mention may be made as isethionates of acylisethionates, such as sodium cocoylisethionate, such as the product sold under the name Jordapon Cl P® by Jordan.j) Mention may be made as taurates of the sodium salt of palm kernel oil methyltaurate, for example sold under the name Hostapon CT Pate® by Clariant; N-acyl-N-methyl taurates, such as sodium N-cocoyl-N-methyltaurate, for example sold under the name Hostapon LT-SF® by Clariant or sold under the name Nikkol CMT-30-T® by Nikkol, or sodium palmitoyl methyltaurate, such as that sold under the name Nikkol PMT® by Nikkol. The preferred is sodium methyl stearoyl taurate (e.g. Nikkol SMT Nikkol). k) Anionic derivatives of alkyl polyglycosides may be in particular citrates, tartrates, sulfosuccinates, carbonates and glycerol ethers obtained from alkyl polyglycosides.Mention may be made, for example, of the sodium salt of cocoylpolyglucoside (1,4) tartaric ester, e.g., sold under the name Eucarol AGE-ET® by Cesalpinia, the disodium salt of cocoylpolyglucoside (1,4) sulfosuccinic ester, such as the product sold under the name Essai 512 MP® by Seppic, or the sodium salt of cocoylpolyglucoside (1,4) citric ester, e.g., sold under the name Eucarol AGEEC® by Cesalpinia. Non-ionic surfactants

[0058] Non-limiting examples of nonionic surfactants that may be used to carry out the present invention include, for example, alkyl and polyalkyl esters of glycerol, such as polyglyceryl-3 dicitrate / stearate; mixtures of alkyl and polyalkyl esters of glycerol with polyglyceryl, such as polyglyceryl-3 methylglucose distearate; oxyalkylenated (more specifically, polyoxyethylenated) fatty acid esters of glycerol; oxyalkylenated fatty acid esters of sorbitan; oxyalkylenated (oxyethylenated and / or oxypropylenated) fatty acid esters; oxyalkylenated (oxyethylenated and / or oxypropylenated) fatty alcohol esters; polyol fatty acid esters (glyceryl stearate and / or sorbitan tristearate, polyethylene glycol 400 monoleate, polyethylene glycol 600 monoleate, polyethylene glycol 400 dioleate, and polyethylene glycol 600 dioleate); polyethylene glycol fatty acid esters (PEG-40 stearate and PEG-100);sugar esters, such as sucrose stearate; fatty alcohol ethers of sugars, especially alkyl polyglycosides (APGs) such as decyl glucoside, lauryl glucoside, cetostearyl glucoside, optionally in the form of a mixture with cetostearyl alcohol, as well as arachidyl glucoside, for example, in the form of a mixture of arachidyl alcohol, behenyl alcohol and arachidyl glucoside. According to a specific embodiment of the present invention, the mixture of the alkyl polyglycoside as defined above with the corresponding fatty alcohol may be in the form of a self-emulsifying composition. Mention; can also be made from lecithins and derivatives (e.g. biophilic), sugar esters and sodium stearoyl lactylate.

[0059] Alkyl esters of glycerol include glyceryl esters of fatty acids, such as glyceryl stearate (glyceryl mono-, di- and / or tristearate), glyceryl laurate or glyceryl ricinoleate and mixtures thereof. As their polyoxyalkylenated derivatives, there may be mentioned mono-, di- or triester of fatty acids with a polyoxyalkylenated glycerol (mono-, di- or triester of fatty acids with a polyalkylene glycol ether of glycerol), preferably polyoxyethylenated glycerol stearate (mono-, di- and / or tristearate), such as PEG20 glyceryl stearate (mono-, di- and / or tristearate).

[0060] Polyglycerol esters of fatty acids may be selected from esters derived from the reaction of polyglycerol having from 2 to 12 glycerol units, preferably from 3 to 10 glycerol units, and at least one fatty acid comprising from 8 to 24 carbon atoms, preferably from 8 to 20 carbon atoms, preferably from 10 to 18 carbon atoms, and most preferably from 10 to 14 carbon atoms. Fatty acids containing from 8 to 24 carbon atoms may be linear or branched, saturated or unsaturated. The fatty acids may be selected from oleic acid, stearic acid, isostearic acid, lauric acid, palmitic acid, myristic acid, linoleic acid, capric acid, caprylic acid, or mixtures thereof.

[0061] According to a preferred embodiment, the fatty acid polyglycerol esters are selected from esters obtained from the reaction of polyglycerol comprising 2 to 12 glycerol units, preferably from 4 to 10 glycerol units, and from at least one fatty acid having less than 16 carbon atoms, preferably less than 15 carbon atoms, for example from 8 to 16 carbon atoms and better still from 8 to 14 carbon atoms.

[0062] According to one embodiment, the polyglycerol fatty acid ester is selected from polyglycerol esters derived from the reaction comprising from 4 to 10 glycerol units and at least one fatty acid comprising from 8 to 12 carbon atoms, preferably 10 to 12 carbon atoms, such as lauric acid and / or capric acid. Mention may be made, for example, of the ester from the reaction of polyglyceryl-10 and lauric acid, or of the ester from the reaction of polyglycerol-4 and capric acid.

[0063] Examples of oxyalkylenated fatty acid esters that may be mentioned include the adducts of ethylene oxide with esters of lauric acid, palmitic acid, stearic acid or behenic acid and mixtures thereof, especially those containing from 9 to 100 oxyethylene units, such as PEG-9 to PEG-50 laurate (PEG-9 laurate to PEG-50 laurate); PEG-9 to PEG-50 palmitate (PEG-9 palmitate to PEG-50 palmitate); PEG-9 to PEG-50 stearate (PEG-9 stearate to PEG-50 stearate); PEG-9 to PEG-50 palmitostearate; PEG-9 to PEG-50 behenate (PEG-9 behenate to PEG-50 behenate); polyethylene glycol monostearate 100 EO (PEG-100 stearate); and mixtures thereof.

[0064] Fatty alcohols suitable for use in the present invention include alcohols having 4 or more, preferably 6 or more, and most preferably 12 or more carbon atoms. The fatty alcohol may be saturated or unsaturated. The fatty alcohol may be linear or branched.

[0065] Fatty alcohols may have the structure R-OH in which R is chosen from saturated and unsaturated, linear and branched radicals containing from 4 to 40 carbon atoms, preferably from 6 to 30 carbon atoms, and more preferably from 12 to 20 carbon atoms. In at least one embodiment, R may be selected from C12-C20 alkyl and C12-C20 alkenyl groups. R may or may not be substituted by at least one hydroxyl group.

[0066] Examples of fatty alcohol include lauryl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, undecylenyl alcohol, myristyl alcohol, octyldodecanol, hexyldecanol, oleyl alcohol, linoleyl alcohol, palmitoleyl alcohol, arachidonyl alcohol, erucyl alcohol, and mixtures thereof.

[0067] The fatty acids used in the context of the invention are more particularly chosen from saturated or unsaturated carboxylic acids with 6 to 30 carbon atoms, preferably 9 to 30 carbon atoms. They are advantageously chosen from myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid and isostearic acid.

[0068] Other examples of nonionic surfactants to be used in the surfactant system of the present invention include polycondensates of ethylene oxide and propylene oxide of the following structure: H-(O-CH2-CH2)a-(O-CH(CH3)-CH2)b-(O-CH2-CH2)a'-OH, where a, a' range from 2 to 150 and b range from 1 to 100.

[0069] As polycondensate products of ethylene oxide and propylene oxide that can be used according to the invention, but are not limited to these, mention may be made of the polyethylene glycol / polypropylene glycol / polyethylene glycol triblock polycondensate products sold under the name Synperonic, for example Synperonic® PE / F32 (INCI name: Poloxamer 108), Synperonic® PE / F108, Synperonic® PE / L44, Synperonic® PE / L42, Synperonic® PE / F127, Synperonic® PE / F88, Synperonic® PE / L64, Synperonic® PE / F88, Synperonic® PE / F87 by Croda, Lutrol® F68 by BASF, Praidmul PEG 400 MO, Praidmul PEG 400 DO, Praidmul PEG 600 DO, by the Praid company. Organic solvents

[0070] At least one organic solvent may be used in the present invention for the production process of C. longa nanostructures using a low-energy input method, selected from: alcohols, ketones, esters, ethers, and hydrocarbons, and mixtures thereof may or may not be used. Examples include, but are not limited to, methanol, ethanol, propanol, ethyl acetate, isopropyl acetate, acetone, propylene glycol, cyclohexanone, water, and ethyl acetate. The addition of an organic solvent is not mandatory. Preservatives

[0071] At least one preservative may be used in the present invention for the production process of C. longa nanostructures by a low-energy input method and is selected from, but not limited to, the following: phenoxyethanol, vitamin C, vitamin E, potassium sorbate, parabens, isothiazolinones, benzyl alcohol, capryl caprylates, benzoic acid, sorbic acid, metal salts, sodium benzoate, triclosan, formaldehyde, ethylhexylglycerin, silver salts, zinc salts, plant essential oils, and mixtures thereof may or may not be used. The addition of preservatives is not mandatory. Thickeners

[0072] At least one thickener can be used in the present invention for the production process of C. longa nanostructures by low energy input method and is selected from: organic thickeners, inorganic thickeners, ionic polymers, nonionic polymers, minerals, and / or modified minerals, and mixtures thereof may or may not be used. Examples include, but are not limited to, acrylates, methacrylates, polyethylene glycols, carbomers, zinc stearate, magnesium-aluminum silicate, aluminum stearate, bentonite, aluminum-magnesium hydroxystearate, silica, algin, carrageenan, agar, hydroxyethylcellulose, starch, cellulose, pectin, gelatin, natural gums, modified gums, synthetic gums, sodium chloride, and mixtures thereof may or may not be used. The addition of thickeners is not mandatory. Nanostructures comprising oil and / or extract of C. longa produced by the process described in the present invention

[0073] The second object of the invention are the nanostructures comprising C. longa oil and / or extract produced by the process described in the present invention, comprising a combination of at least three components: C. longa extract and / or oil, at least one surfactant, and water. At least one organic solvent and / or at least one preservative and / or at least one fragrance / aroma / flavoring / perfume and / or at least one thickener may also be added. Typically, the nanostructures are dispersed in an aqueous vehicle that is cosmetically acceptable. More specifically, the compositions may comprise: a. C. longa extract and / or oil, b. At least one surfactant; c. Water; d. At least one organic solvent, the addition being optional; e. At least one preservative, the addition being optional; f. At least one fragrance / aroma / flavoring / perfume, the addition being optional; g. At least one thickener, the addition being optional.

[0074] The concentration of the components of the nanostructures produced according to this invention is: a. Between 0.01% and 20% by weight of C. longa extract and / or oil, b. Between 0.01% and 20% by weight of at least one surfactant; c. Between 60% and 99.99% by weight of water; d. Between 0.1% and 20% by weight of at least one organic solvent, the addition being optional; e. Between 0.001 and 5% by weight of at least one preservative, the addition being optional; f. Between 0.001 and 5% by weight of at least one fragrance / aroma / flavoring / perfume, the addition being optional; g. Between 0.1 and 10% by weight of at least one thickener, the addition being optional.

[0075] To evaluate the formation and stability of nanostructures, qualitative and quantitative analyses were performed.

[0076] For qualitative analyses, the presence of the Tyndall effect, the lack of phase separation and the lack of formation of precipitates were observed.

[0077] The presence of the Tyndall effect is a visual indication to the naked eye of the formation of nanostructures. It is an optical phenomenon of visible light scattering by colloidal suspensions in which nanometric particles dispersed in a colloidal solution with dimensions ranging from 1 to hundreds of millimeters Nanometers exhibit a bluish or greenish reflection. This effect provides us with a rapid technique for visually characterizing nanometric colloidal systems.

[0078] Phase separation and the formation of precipitates are signs to the naked eye of loss of formulation stability.

[0079] For quantitative analysis, particle size and polydispersity index (PI) were analyzed. These are parameters indicative of nanostructure formation and stability.

[0080] Particle size can affect stability, release kinetics, carrying capacity, and toxicity. To be considered nanosized, a particle size must be smaller than 1000nm (one thousand nanometers).

[0081] The PI indicates the average size distribution of nanostructures, indicating the homogeneity or heterogeneity of the formulation in relation to dispersion. The scale ranges from 0.000 to 1.000, with values ​​closer to zero indicating a more homogeneous formulation and values ​​closer to one indicating a more heterogeneous formulation.

[0082] The nanostructures presented particle sizes between 30 and 600 nm and IP between 0.030 and 0.650, being evaluated over a period of 30 days, proving to be stable (Figure 1).

[0083] Thus, the invention provides nanostructures comprising oil and / or extract of C. longa by the process described in the present invention obtaining stable nanostructures.

[0084] The invention also provides nanostructures comprising oil and / or extract of C. longa by the process described in the present invention where the nanostructures produced allow greater permeation of the active ingredients due to the small diameter.

[0085] The invention also provides nanostructures comprising oil and / or extract of C. longa by a process described in the present invention in which the nanostructures produced enable the increase in the effectiveness of action due to gradual release, thus increasing the contact time with the surface.

[0086] The invention also provides nanostructures comprising oil and / or extract of C. longa by the process described in the present invention in which the nanostructures produced enable the increase of the physical stability of the formulation.

[0087] The invention also provides nanostructures comprising oil and / or extract of C. longa by a process described in the present invention in which the nanostructures produced enable the protection of the active ingredients (curcuminoids) from degradation both of the formulation and of external environments. Cosmetic composition comprising the nanostructures comprising oil and / or extract of C. longa produced by the process of the present invention

[0088] The third object of the invention is the cosmetic composition for application to the skin, hair and nails comprising the nanostructures comprising oil and / or extract of C. longa obtained by the process described in the present invention.

[0089] In addition to the nanostructures comprising C. longa oil and / or extract produced by the process of the present invention, the cosmetic composition may contain at least one polymer and water. Polymers

[0090] Suitable additional polymers according to the present invention are selected from rheology modifying polymers, non-ionic polymers and ionic polymers which may be water-soluble or water-dispersible.

[0091] Ionic polymers can be polymers with ionic groups distributed throughout the polymer backbone. Ionic groups, which may include carboxylate, sulfonate, sulfate, phosphate, nitrate, or other negatively charged or ionizable groups, may be arranged on pendant groups from the backbone or may be incorporated into the backbone itself.

[0092] The ionic polymers may comprise at least one hydrophilic unit of the olefinic unsaturated carboxylic acid type and at least one hydrophobic unit exclusively of alkyl (C10-C30) ester of the unsaturated carboxylic acid type.

[0093] In certain non-limiting examples and embodiments, the copolymers are selected from copolymers resulting from the polymerization of: (1) at least one monomer of formula (I): CH2=CH(R1)COOH (I) where R1 is selected from H, CH3 or C2H5, which provides acrylic acid, methacrylic acid or ethacrylic acid monomers; and (2) at least one alkyl (C10-C30) ester monomer of the unsaturated carboxylic acid type, which corresponds to the monomer of formula (II): CH2=CH(R2)COOR3(II)

[0094] Furthermore, crosslinked polymers may be selected according to further exemplary embodiments. Such polymers may be selected, for example, from polymers resulting from the polymerization of a mixture of monomers comprising: a. acrylic acid; b. an ester of formula (II) described above, wherein R2 is selected from H or CH3 and R3 denotes an alkyl radical containing 12 to 22 carbon atoms; c. a crosslinking agent, which is a known copolymerizable polyethylene unsaturated monomer, such as diallyl phthalate, allyl (meth)acrylate, divinylbenzene, (poly)ethylene glycol dimethacrylate, and methylenebisacrylamide.

[0095] Suitable polymers, then, include, but are not limited to: polylactic acid (PLA), xanthan gum, poly-C10-30 alkyl acrylate, acrylates / C10-C30 alkyl acrylate cross-linked polymer, styrene / acrylates copolymer, lauryl methacrylate / glycol dimethacrylate cross-linked polymer, ammonium acryloyldimethyltaurate / VP copolymer, dimethicone / vinyl dimethicone cross-linked polymer, taurate polymers, acrylates / vinyl isodecanoate cross-polymer, acrylates / C10-30 alkyl acrylate cross-polymer, carbomers, ammonium acryloyldimethyltaurate / VP cross-polymer, ammonium acryloyldimethyltaurate / beheneth-25 methacrylate cross-polymer, acrylates / ceteth-20 itaconate, polyacrylamide, nonionic thickener, acrylate / C10-C30 alkyl acrylate copolymers, natrosol, carbopol, carboxymethyl cellulose, polyacrylic acid, polymethacrylic acid, carboxyvinyl polymer,acrylic acid / vinyl ester / acrylates copolymer / nyl isodecanoate, acrylates / palmeth-25 acrylate copolymer, steareth-20 acrylate / itaconate copolymer and Celeth-20 acrylate / itaconate copolymer, sulfonate polymers, polysulfonic acid, sodium polystyrene sulfonate, methacrylic acid and acrylamidomethylpropane sulfonic acid copolymers and acrylic acid and acrylamidomethylpropane sulfonic acid copolymers, guar gum, carboxy, copolymers of ethylene and maleic acid, acrylate polymer, silicone, carbomer, sodium carboxymethyl cellulose, acrylate copolymer, acrylates copolymer, acrylates crosspolymer 4, hydroxyethyl cellulose, alkyl (C10-C30) esters of unsaturated carboxylic acids, lauryl acrylate, stearyl acrylate, decyl acrylate, isodecyl acrylate, dodecyl acrylate and the corresponding methacrylates such as lauryl methacrylate, stearyl methacrylate, decyl methacrylate, isodecyl methacrylate, dodecyl methacrylate, nonionic polymers, ionic polymers and mixtures thereof.

[0096] Examples of non-ionic polymers may be the following, but not limited to: a. hydroxyethyl cellulose, b. celluloses modified by groups comprising at least one fatty chain; examples that may be mentioned include: - hydroxyethylcelluloses modified by groups comprising at least one fatty chain, such as alkyl, arylalkyl or alkylaryl groups, or mixtures thereof, in which the alkyl groups are preferably C8-C22; methyl hydroxyethylcellulose; methyl ethyl hydroxyethylcellulose; or hydroxypropyl cellulose, - hydroxyethylcelluloses modified by alkylphenyl polyalkylene glycol ether groups, c. hydroxypropyl guars, such as hydroxypropyl guar and hydroxypropyl guars modified by groups comprising at least one fatty chain. Additional ingredients

[0097] In addition to the essential components described above, the cosmetic composition according to the present invention may additionally comprise any usual cosmetically acceptable ingredient, called pharmacotechnical adjuvants, which may be specially selected from antioxidants, fragrances / aromas / flavorings / perfumes, chelators, sweeteners, preservatives, solvents, actives, vitamins, surfactants, fatty compounds, vitamins, pigments, additional fillers, silicones, polymers and mixtures thereof.

[0098] Technical persons will select the optional additional ingredients and / or their amounts so that the advantageous properties of the composition according to the present invention are not, or are not substantially, affected by the addition.

[0099] Non-limiting examples of preservatives and antioxidants that can be used according to the invention include phenoxyethanol, vitamin C, vitamin E, potassium sorbate, parabens, isothiazolinones, benzyl alcohol, capryl caprylates, benzoic acid, sorbic acid, metal salts, sodium benzoate, triclosan, formaldehyde, ethylhexylglycerin, silver salts, zinc salts, plant essential oils and mixtures thereof.

[0100] Suitable additional fillers of the invention may be, but are not limited to: mica, silica, Oryza sativa (corn) starch, Oryza sativa (rice) starch, magnesium oxide, nylon 12, nylon 66, polyethylene, pumice, cellulose, talc, methicone, dimethicone, perlite, sodium silicate, PTFE, polymethyl methacrylate, alumina, calcium sodium borosilicate, magnesium carbonate, aluminum starch octenylsuccinate, modified potato starch, alumina, silica silylate, hydrated silica, dimethicone / vinyl dimethicone crosspolymer, sodium carboxylmethyl starch, and mixtures thereof.

[0101] Fat and / or oily materials and / or fatty compounds that can be added in the present invention, but not limited to these, are: esters, fatty acids, synthetic oils and hydrocarbons / paraffins, such as stearyl alcohol, myristic acid, palmitic acid, silicones, mineral oil, dicaprylyl carbonate, diisopropyl sebacate, stearyl alcohol, vegetable / plant oils and their mixtures.

[0102] Additional active ingredients include, but are not limited to: disodium EDTA, triethanolamine, vitamin C, vitamin E, niacinamide, plant extracts and / or vegetable oils and their mixtures.

[0103] Silicones that can be used, but are not limited to these, are: dimethicone, cyclohexasiloxane, caprylyl methicone and their mixtures.

[0104] Non-limiting example of vitamins suitable for the composition of the present invention include tocopherol and ascorbic acid and mixtures thereof.

[0105] Suitable solvents include, but are not limited to: water, alcohols, ketones, glycols and polyols, such as glycerin, water, caprylyl glycol, pentylene glycol, propylene glycol, butylene glycol, C12-15 alkyl benzoate, and mixtures thereof.

[0106] In various embodiments, the solvent is present in a concentration of about 1 to 99% by weight, including intermediate ranges and subranges, based on the total weight of the combinations and / or compositions according to the present invention. Examples of embodiments of the invention

[0107] By way of non-limiting illustration, the present invention will now be described with reference to the following examples. Example 1 Preparation of a formulation of C. longa nanostructures produced by the process described in this invention

[0108] The method for preparing nanostructures comprising C. longa oil and / or extract may be, for example, as follows: production of an organic phase (OP) composed of C. longa extract and / or oil at a final concentration of approximately 0.01% by weight to approximately 10% by weight and organic solvent. The aqueous phase (AP) is composed of water at a final concentration of approximately 80% by weight to approximately 99.99% by weight. At least one preservative and / or at least one thickener and / or at least one fragrance / aroma / flavoring / perfume, depending on their solubility characteristics, may be optionally added to the OP and / or AP, with constant stirring until completely homogenized, to improve sensory and / or preservation characteristics. Subsequently, the AP is slowly added to the OP with constant stirring until complete addition and homogenization. The process may also occur in reverse, that is, adding the OP to the AP with constant stirring until complete addition and homogenization.Afterwards, the organic solvent can be removed through solvent evaporation processes, such as rotary evaporators, ovens, natural evaporation, pneumatic concentrators, etc. This last step is optional.

[0109] It is possible to observe the presence of the Tyndall effect, a bluish reflection, which is considered an indication of the presence of nanostructures.

[0110] Regarding size, the nanostructures present average diameters smaller than 300 nm after 7 days of preparation, as shown in Table 2. The standard deviations obtained are small, demonstrating repeatability. These results demonstrate the stability of the developed formulations.

[0111] Regarding the IP, the nanostructures present a value lower than 0.400 throughout the analyzed period (0 to 7 days), showing that they are not very polydisperse, as shown in Table 2. TABLE 2. Particle size distribution and IP results for nanostructures prepared with C. longa extract from example 1. Results expressed as the mean ± SD of three readings. Example 2 Preparation of a formulation of C. longa nanostructures produced by the process described in this invention

[0112] The method for preparing nanostructures comprising C. longa oil and / or extract may be, for example, as follows: production of an organic phase (OP) composed of C. longa extract and / or oil at a final concentration of approximately 0.01% to approximately 10% by weight, surfactant at a final concentration of approximately 0.01% to approximately 10% by weight, and organic solvent for dilution. The aqueous phase (AP) is composed of water at a final concentration of approximately 80% to approximately 99.99% by weight. At least one preservative and / or at least one thickener and / or at least one fragrance / aroma / flavoring / perfume, depending on their solubility characteristics, may be optionally added to the OP and / or AP, with constant stirring until completely homogenized, to improve the sensory and / or preservation characteristics. Subsequently, the AP is slowly added to the OP with constant stirring until complete addition and homogenization.The process can also occur in reverse, that is, by adding FO to the FA with constant stirring until complete addition and homogenization. Subsequently, the organic solvent can be removed through solvent evaporation processes, such as rotary evaporators, ovens, natural evaporation, pneumatic concentrators, fans, etc. This last step is not mandatory and is therefore optional.

[0113] It is possible to observe the presence of the Tyndall effect, a bluish reflection, which is considered an indication of the presence of nanostructures.

[0114] Regarding the size of the nanostructures obtained by this method comprising C. longa and surfactants, it is possible to observe that they presented sizes smaller than 200 nm and with similar values ​​regardless of the times analyzed, showing stability and reproducibility (Table 3).

[0115] Regarding the IP of the nanostructures obtained by this method comprising C. longa and surfactants, it is possible to observe that they presented IP values ​​lower than 0.300 and with similar values ​​regardless of the times analyzed, showing stability and reproducibility (Table 3). TABLE 3. Particle size distribution and IP results for nanostructures prepared with C. longa extract from example 2. Results expressed as the mean ± SD of three readings. Example 3 to 5 Cosmetic formulation comprising a formulation of C. longa nanostructures produced by the process described in this invention

[0116] Cosmetic compositions according to the present invention are shown as examples 3 to 5, as follows in Table 4. TABLE 4. Examples of cosmetic compositions comprising a formulation of C. longa nanostructures produced by the process described according to the present invention. Example 6 Color transfer test to skin

[0117] The invention also provides nanostructures comprising C. longa oil and / or extract using a low-energy input method where the nanostructures produced mask the yellow coloration of the active ingredient C. longa on the skin.

[0118] The first evaluation of the non-transfer of the yellow color of C. longa by the nanostructures produced by the method described in the present invention was made by applying two formulations for comparison to the participant's forearm: a. formulation of nanostructures comprising oil and / or extract of C. longa produced by the process described in the following invention on the forearm; and b. formulation of oil and / or extract of pure C. longa (at the same concentration as the nanostructure).

[0119] The color masking analysis was performed qualitatively, verifying the presence or absence of yellow on the skin. It was possible to observe that there was no transfer of the yellow color to the skin by the nanostructures produced by the process described in the present invention and that the oil and / or extract of C. longa there was indeed a transfer of the yellow color. The records are shown in Figure 2.

[0120] The second assessment of the lack of transfer of the yellow color of C. longa by the nanostructures produced by the process described in this invention was made in relation to the time of application. To this end, the nanostructures produced by this invention were applied daily to the forearm of the participant (who had suspended the use of any topical products on the forearms for 48 hours before the start of the study) for 7 days. The color masking analysis was performed qualitatively, verifying the presence or absence of the yellow color on the skin. It was possible to observe that there was no transfer of the yellow color to the skin. References ALEXIS, Frank; PRIDGEN, Eric; MOLNAR, Linda K.; FAROKHZAD, Omid C. Factors affecting the clearance and biodistribution of polymeric nanoparticles. Molecular Pharmaceutics, [SI], v. 5, no. 4, p. 505-515, 2008. ISSN: 15438384. DOI: 10.1021 / mp800051m. AMMON, HPT; WAHL, MA Pharmacology of Curcuma longa. 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Disponível em: https: / / doi.Org / 10.1016 / j.indcrop.2021.114050. ZAMARIOLI, Cristina M.; MARTINS, Rodrigo M.; CARVALHO, Emilia C.; FREITAS, Luis A. P. Nanoparticles containing curcuminoids (Curcuma longa): Development of topical delivery formulation. Revista Brasileira de Farmacognosia, [S. I.], v. 25, n. 1 , p. 53-60, 2015. ISSN: 1981528X. DOI: 10.1016 / j.bjp.2014.11 .010. Disponível em: http: / / dx.doi.Org / 10.1016 / j.bjp.2014.11.010.

Claims

CLAIMS 1. PROCESS FOR OBTAINING NANOSTRUCTURES COMPRISING Curcuma longa, characterized by comprising the following steps: a. preparing an organic phase (OP) by mixing the extract and / or oil of Curcuma longa with at least one surfactant until total homogenization; optionally adding at least one organic solvent for dilution of the extract and / or oil of Curcuma longa being added to the OP (step a); b. preparing an aqueous phase (AP) composed of water; c. optionally adding to the OP and / or the AP at least one preservative and / or at least one thickener and / or at least one fragrance / aroma / flavoring / perfume that will be added depending on its solubility characteristic, in constant stirring until total homogenization; d. adding the AP (b) slowly to the OP (a) or OP (a) slowly to the AP (b) in constant stirring until total homogenization; e. optionally evaporating the organic solvent; f. obtaining the nanostructures comprising Curcuma longa.

2. PROCESS, according to claim 1, characterized by the fact that: a. that the amount of extract and / or oil of Curcuma longa must be between 0.01 and 20% by weight (% by weight is based on the total weight of the homogenate), including all intermediate ranges and subranges; b. having at least one ionic and / or nonionic surfactant, their combinations and / or mixtures, in which the amount of surfactant must be between 0.01 and 20% surfactant by weight (% by weight is based on the total weight of the homogenate), including all intermediate ranges and subranges; c. that the amount of water must be between 60 and 99.99% by weight (% by weight is based on the total weight of the homogenate), including all intermediate ranges and subranges; d.which may additionally have at least one organic solvent and be selected from: alcohols, ketones, esters, ethers, hydrocarbons, water, and their combinations, for diluting the extract and / or oil of Curcuma longa comprising between 0.1% and 20% by weight, including all intermediate ranges and subranges; e. which may additionally have at least one thickener and be selected from organic, inorganic thickeners, ionic polymers, non-ionic polymers, minerals and / or modified minerals, comprising between 0.1 and 10% by weight, including all intermediate ranges and subranges; f. which may additionally have acceptable ingredients selected from preservatives and / or fragrances / aromas / flavorings / perfumes comprising between 0.001 and 5% by weight each, including all intermediate ranges and subranges.

3. PROCESS, according to any one of claims 1 and 2, characterized by the fact that it is a low energy production method and is therefore considered a green method.

4. NANOSTRUCTURES OF Curcuma longa, obtained by the process as defined in any one of claims 1 to 3, characterized by the fact that: a. that the amount of extract and / or oil of Curcuma longa is between 0.01 and 20% by weight, including all intermediate ranges and subranges; b. having at least one ionic and / or non-ionic surfactant, their combinations and mixtures, in which the amount is between 0.01 and 20% by weight, including all intermediate ranges and subranges; c. that the amount of water is between 60 and 99.99% by weight, including all intermediate ranges and subranges.

5. Curcuma longa NANOSTRUCTURES, according to claim 4, characterized by the fact that they can comprise acceptable ingredients such as: a. at least one organic solvent for diluting the extract and / or oil of Curcuma longa selected from alcohols, ketones, esters, ethers, hydrocarbons, water and their combinations, comprising between 0.1 and 20% by weight, including all intermediate ranges and subranges; b. at least one thickener and be selected from organic, inorganic thickeners, ionic polymers, non-ionic polymers, minerals and / or modified minerals, comprising between 0.1 and 10% by weight, including all intermediate ranges and subranges; c. at least one preservative and / or fragrance / aroma / flavoring / perfume comprising between 0.001 and 5% by weight each, including all intermediate ranges and subranges.

6. NANOSTRUCTURES OF Curcuma longa, according to claims 4 and 5, characterized by the fact that they have a particle size between 30 and 600 nm and a polydispersity index (PI) between 0.030 and 0.

650.

7. NANOSTRUCTURES OF Curcuma longa, according to claims 4 to 6, characterized by the fact that they do not transfer the yellow color of Curcuma longa to the skin, hair and nails.

8. COSMETIC COMPOSITION, characterized by the fact that it comprises the nanostructures of Curcuma longa, as defined in any one of claims 4 to 7.

9. COSMETIC COMPOSITION, according to claim 8, characterized by comprising at least one polymer from rheology modifying polymers and / or ionic polymers and additionally cosmetically acceptable ingredients selected from water, antioxidants, perfumes / fragrances / aromas, preservatives, flavorings, chelating agents, sweeteners, emollients, humectants, solvents, actives, surfactants, fatty compounds, emulsifiers, thickeners, sequestrants, vitamins, fillers, silicones, polymers, pigments and their mixtures.

10. COSMETIC COMPOSITION, according to claims 8 and 9, characterized by the fact that it does not transfer the yellow color of Curcuma longa to the skin, hair and nails.

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